Catalytic cracking is the main method to lighten heavy crude oil,this process can produce high quality oil products such as gasoline and diesel,but also produces a large amount of fluid catalytic cracking slurry(FCCS)...Catalytic cracking is the main method to lighten heavy crude oil,this process can produce high quality oil products such as gasoline and diesel,but also produces a large amount of fluid catalytic cracking slurry(FCCS).The catalyst particles in FCCS seriously restrict the secondary processing of FCCS and need to be removed,and the properties of Fccs is an important factor that affects the removal efficiency of the catalyst particles.Based on the"effective contact point"model proposed by the research group,this study further proposed the"electrostatic separation efficiency calculation"model.In this model,since Fccs has a uniform distribution of catalyst particles,the ratio of the number of catalyst particles can be expressed as the ratio of area to achieve the calculation of separation efficiency.Then the catalyst removal efficiency under different viscosity was analyzed,thus verifying the feasibility of this model.The effects of temperature and mass ratio of four components on the viscosity of FccS were investigated respectively,then the effects of temperature and four components'mass ratio on the electrostatic sep-aration can be directly converted into the effect of viscosity on the electrostatic separation efficiency.All the results show the electrostatic separation efficiency decreases with increasing viscosity,and the best separationtemperatureis120℃.展开更多
AIM:Tocomparetheeffectsandcomplicationsof direc cyclopexy (DC) versus vitrectomy, endophotocoagulation, and gas/silicone oil endotamponade (VEE) treatment in patients with cyclodialysis and persistent hypotony.METHODS...AIM:Tocomparetheeffectsandcomplicationsof direc cyclopexy (DC) versus vitrectomy, endophotocoagulation, and gas/silicone oil endotamponade (VEE) treatment in patients with cyclodialysis and persistent hypotony.METHODS: This is a prospective, comparative, non-randomized clinical trial which includes 52 patients with cyclodialysis and persisting hypotony. Fifty-two patients suffering from cyclodialysis and persistent hypotony in one eye were divided into 2 groups (groups DC and VEE) and treated, respectively, with direct cyclopexy or vitrectomy, endophotocoagulation, and gas/silicone oil endotamponade. The patients were followed up for 12 months. Assessments included best corrected visual acuity (BCVA), intraocular pressure (IOP), anterior chamber depth (ACD), anterior chamber volume (ACV) and subjective rating of the pain caused by the treatments.RESULTS: After a follow-up of 12 months, significan improvement was seen in postoperative mean BCVA, IOP, ACD and ACV in both treatment groups (which were not significantly different from each other). The success rates for the treatments were not significantly different (DC: 50.0% vs VEE: 62.5% , P =0.383). Postoperative morbidity of cataract and subjective pain rating were significantly higher in the VEE group vs the DC group (P =0.003 and P <0.001 respectively).CONCLUSION: DC and VEE were effective surgica procedures in treating patients with cyclodialysis and persistent hypotony. Patients had better tolerance to DC treatment and VEE was more likely lead to cataract complications. Taking into consideration the ease of the operation, success rate, and patient comfort, DC treatment seems preferable to VEE treatment in patients with simple cyclodialysis. While VEE has the advantage of treating patients with cyclodialysis combined with vitreous hemorrhage.展开更多
● AIM: To investigate the retinal photoreceptor differentiation potential of human orbital adipose tissue-derived stem cells(ADSCs) generated by enzyme(EN) and explant(EX) culture methods.● METHODS: We investigated ...● AIM: To investigate the retinal photoreceptor differentiation potential of human orbital adipose tissue-derived stem cells(ADSCs) generated by enzyme(EN) and explant(EX) culture methods.● METHODS: We investigated potentials of human orbital ADSCs to differentiate into photoreceptors through EN and EX culture methods. EN and EX orbital ADSCs were obtained from the same donor during rehabilitative orbital decompression, and then were subject to a 3-step induction using Noggin, DKK-1, IGF-1 and b-FGF at different time points for 38 d. Stem cell, eye-field and photoreceptor-related gene and protein markers were measured by reverse transcription-polymerase chain reaction(RT-PCR) and immunofluorescent(IMF) staining. ● RESULTS: Both EX and EN orbital ADSCs expressed CD133, a marker of cell differentiation. Moreover, PAX6 and rhodopsin, markers of the retinal progenitor cells, were detected from EX and EN orbital ADSCs. In EX orbital ADSCs, PAX6 m RNA was detected on the 17^(th) day and then the rhodopsin m RNA was detected on the 24^(th) day. In contrast, the EN orbital ADSCs expressed PAX6 and rhodopsin m RNA on the 31^(st) day. EX orbital ADSCs expressed rhodopsin protein on the 24^(th) day, while EN orbital ADSCs expressed rhodopsin protein on the 31^(st) day.● CONCLUSION: Orbital ADSCs isolated by direct explants culture show earlier and stronger expressions of markers towards eye field and retinal photoreceptor differentiation than those generated by conventional EN method.展开更多
BACKGROUND: Retinoid X receptor(RXR) plays a central role in the regulation of intracellular receptor signaling pathways. The activation of RXR has protective effect on H2O2-induced apoptosis of H9c2 ventricular cells...BACKGROUND: Retinoid X receptor(RXR) plays a central role in the regulation of intracellular receptor signaling pathways. The activation of RXR has protective effect on H2O2-induced apoptosis of H9c2 ventricular cells in rats. But the protective effect and mechanism of activating RXR in cardiomyocytes against hypoxia/reoxygenation(H/R)-induced oxidative iniury are still unclear.METHODS: The model of H/R injury was established through hypoxia for 2 hours and reoxygenation for 4 hours in H9c2 cardiomyocytes of rats. 9-cis-retinoic acid(9-cis RA) was obtained as an RXR agonist, and HX531 as an RXR antagonist. Cultured cardiomyocytes were randomly divided into four groups: sham group, H/R group, H/R+9-cis RA-pretreated group(100 nmol/L 9-cis RA), and H/R+9-cis RA+HX531-pretreated group(2.5 μmol/L HX531). The cell viability was measured by MTT, apoptosis rate of cardiomyocytes by flow cytometry analysis, and mitochondrial membrane potential(ΔΨm) by JC-1 fluorescent probe, and protein expressions of Bcl-2, Bax and cleaved caspase-9 with Western blotting. All measurement data were expressed as mean±standard deviation, and analyzed using one-way ANOVA and the Dunnett test. Differences were considered signif icant when P was <0.05.RESULTS: Pretreatment with RXR agonist enhanced cell viability, reduced apoptosis ratio, and stabled ΔΨm. Dot blotting experiments showed that under H/R stress conditions, Bcl-2 protein level decreased, while Bax and cleaved caspase-9 were increased. 9-cis RA administration before H/R stress prevented these effects, but the protective effects of activating RXR on cardiomyocytes against H/R induced oxidative injury were abolished when pretreated with RXR pan-antagonist HX531.CONCLUSION: The activation of RXR has protective effects against H/R injury in H9c2 cardiomyocytes of rats through attenuating signaling pathway of mitochondria apoptosis.展开更多
Backgroud:The electroacupuncture of auricular concha area(EA-ACR)proved to have a good regulatory effect on insomnia and the release of melatonin,which is most closely related to sleep.However,there is little research...Backgroud:The electroacupuncture of auricular concha area(EA-ACR)proved to have a good regulatory effect on insomnia and the release of melatonin,which is most closely related to sleep.However,there is little research to demonstrate the relationship between EA-ACR and melatonin.The objective of present study is to observe the effects of EA-ACR on the circadian rhythm of melatonin secretion in p-chlorophenylalanine(PCPA)-induced insomnia rats.Methods:The study divided 36 male Sprague-Dawley rats randomly into three groups:12 in blank group,12 in model group,and 12 in EA-ACR group.By using PCPA intraperitoneal injection for 2 days,insomnia model was established.EA-ACR group was treated for 28 days.We observed the effects of EA-ACR on the rats'behavioral changes,open-field test and weight during the experiment.After the experiment,the rat tail and heart blood were collected,and the melatonin was detected by enzyme-linked immunosorbent assay.Results:Compared with the blank group,28–30 hours after PCPA intraperitoneal injection,the circadian rhythm of sleep and the total sleep time of model rats decreased.Compared with the model group,electrical stimulation in the ear concha region could effectively reverse the behavioral changes of rats,and increase the trend of weight growth(P=0.010),the crossing score(P=0.014),vertical motion score(P=0.015)and the level of plasma melatonin secretion(P=0.020)with the trend showing a rhythmic pattern(0–60 minutes upward,60–120 minutes downward,120–150 minutes upward).Conclusion:EA-ACR can improve the quality of life(weight),ameliorate exploring irritable and anxiety behaviors in PCPA-induced insomnia rats.The underlying effects of EA-ACR may be excited on the circadian rhythm of melatonin secretion in PCPA-induced insomnia rats.展开更多
An impedance matched parametric amplifier(IMPA)with Josephson junctions is fabricated and characterized.A hybrid structure containing coplanar and strip structures is implemented to realize an impedance taper line and...An impedance matched parametric amplifier(IMPA)with Josephson junctions is fabricated and characterized.A hybrid structure containing coplanar and strip structures is implemented to realize an impedance taper line and a plate capacitor in an LC nonlinear resonator based on Josephson junctions.The upper plate of the capacitor is isolated with SiNx without grounding as well as the strips.Such easily-prepared designs greatly reduce the requirements for lithography alignment and precision,which makes the fabrication process more reliable.The experimental results show that in such IMPA a gain higher than 25 dB with a bandwidth of about 100 MHz can be obtained.This broadband amplifier operates close to the quantum limit.By adjusting the working point,a higher bandwidth of about 400 MHz can be obtained with a gain of about 17 dB.展开更多
We design and fabricateλ/2 coplanar waveguide NbN resonators,the thickness and length of which are only several nanometers and hundred microns,respectively.The quality factor of such compact resonators can reach up t...We design and fabricateλ/2 coplanar waveguide NbN resonators,the thickness and length of which are only several nanometers and hundred microns,respectively.The quality factor of such compact resonators can reach up to 7.5×10~4 at single photon power level at 30 m K with the resonance frequency around 6.835 GHz.In order to tune the resonant frequency,the resonator is terminated to the ground with a dc-SQUID.By tuning the magnetic flux in the dc-SQUID,the effective inductance of the dc-SQUID is varied,which leads to the change in the resonant frequency of the resonator.The tunability range is more than 30 MHz and the quality factor is about 3×10~3.These compact and tunable NbN resonators have potential applications in the quantum information processing,such as in the precision measurement,coupling and/or reading out the quantum states of qubits.展开更多
Insomnia has become one of the common clinical diseases.In recent years,it has been found that patients with insomnia of Yang deficiency type are more and more common,and the trend of rejuvenation gradually appears,wh...Insomnia has become one of the common clinical diseases.In recent years,it has been found that patients with insomnia of Yang deficiency type are more and more common,and the trend of rejuvenation gradually appears,which seriously affects the normal progress of people's life,work and study.Clinical treatment through syndrome differentiation,mainly from the heart,spleen,kidney three viscera yang deficiency,on the basis of the warm yang method to add Qianzhen tranquilizing products,and pay attention to multiple organs as diseases,add moisture,blood The qi thing,while not forgetting the psychological counseling,adjusting the body and mind,and achieving a certain clinical effect,can provide ideas for the treatment of this disease.展开更多
BACKGROUND Due to the complexity and numerous comorbidities associated with Crohn’s disease(CD),the incidence of postoperative complications is high,significantly impacting the recovery and prognosis of patients.Cons...BACKGROUND Due to the complexity and numerous comorbidities associated with Crohn’s disease(CD),the incidence of postoperative complications is high,significantly impacting the recovery and prognosis of patients.Consequently,additional stu-dies are required to precisely predict short-term major complications following intestinal resection(IR),aiding surgical decision-making and optimizing patient care.AIM To construct novel models based on machine learning(ML)to predict short-term major postoperative complications in patients with CD following IR.METHODS A retrospective analysis was performed on clinical data derived from a patient cohort that underwent IR for CD from January 2017 to December 2022.The study participants were randomly allocated to either a training cohort or a validation cohort.The logistic regression and random forest(RF)were applied to construct models in the training cohort,with model discrimination evaluated using the area under the curves(AUC).The validation cohort assessed the performance of the constructed models.RESULTS Out of the 259 patients encompassed in the study,5.0%encountered major postoperative complications(Clavien-Dindo≥III)within 30 d following IR for CD.The AUC for the logistic model was 0.916,significantly lower than the AUC of 0.965 for the RF model.The logistic model incorporated a preoperative CD activity index(CDAI)of≥220,a diminished preoperative serum albumin level,conversion to laparotomy surgery,and an extended operation time.A nomogram for the logistic model was plotted.Except for the surgical approach,the other three variables ranked among the top four important variables in the novel ML model.CONCLUSION Both the nomogram and RF exhibited good performance in predicting short-term major postoperative complic-ations in patients with CD,with the RF model showing more superiority.A preoperative CDAI of≥220,a di-minished preoperative serum albumin level,and an extended operation time might be the most crucial variables.The findings of this study can assist clinicians in identifying patients at a higher risk for complications and offering personalized perioperative management to enhance patient outcomes.展开更多
Background:Mutations in the isocitrate dehydrogenase 1(IDH1)and IDH2 genes are important for both the integrated diagnosis and the prognosis of diffuse gliomas.The p.R132H mutation of IDH1 is the most frequently obser...Background:Mutations in the isocitrate dehydrogenase 1(IDH1)and IDH2 genes are important for both the integrated diagnosis and the prognosis of diffuse gliomas.The p.R132H mutation of IDH1 is the most frequently observed IDH mutation,while IDH2 mutations were relatively rarely studied.The aim of the study was to determine the pathological and genetic characteristics of lowergrade gliomas that carry IDH2 mutations.Methods:Data from 238 adult patients with lower-grade gliomas were retrospectively analyzed.The status of IDH1/2 gene mutations,telomerase reverse transcriptase(TERT)promoter mutations,O^6-methylguanine-DNA-methyltransferase(MGMT)promoter methylation,1p/19q co-deletion and the expressions of IDH1 R132H,alpha-thalassemia X-linked mental retardation,and p53 were evaluated.Progression-free survival(PFS)and overall survival(OS)were calculated via Kaplan-Meier estimation using the log-rank test.Results:Totally,71%(169/238)of patients were positive for IDH mutations,including 12 patients harboring mutations in IDH2.Among the 12 patients with IDH2 mutations,ten patients harbored the R172K mutation,one patient harbored the R172S mutation and one harbored the R172W mutation.Of these,11 tumors occurred in the frontal lobe and showed morphology typical of oligodendroglioma.The proportion of grade II tumors was higher than that of grade III tumors in IDH2 mutant-gliomas.IDH2 mutations were frequently associated with TERT promoter mutations,1p/19q co-deletion and MGMT promoter methylation.IDH2 mutations were associated with better outcomes compared with IDH wild-type gliomas(P<0.05).However,the PFS and OS did not differ from that of IDH1 mutant patients(P=0.95 and P=0.60,respectively).Conclusions:IDH2 mutations are more frequent in oligodendrogliomas and associated with a better prognosis.IDH2 mutations may segregate in distinct clinico-pathological and genetic subtypes of gliomas,and therefore may merit routine investigation.展开更多
Thalidomide inducesγ-globin expression in erythroid progenitor cells,but its efficacy on patients with transfusion-dependentβ-thalassemia(TDT)remains unclear.In this phase 2,multi-center,randomized,double-blind clin...Thalidomide inducesγ-globin expression in erythroid progenitor cells,but its efficacy on patients with transfusion-dependentβ-thalassemia(TDT)remains unclear.In this phase 2,multi-center,randomized,double-blind clinical trial,we aimed to determine the safety and efficacy of thalidomide in TDT patients.A hundred patients of 14 years or older were randomly assigned to receive placebo or thalidomide for 12 weeks,followed by an extension phase of at least 36 weeks.The primary endpoint was the change of hemoglobin(Hb)level in the patients.The secondary endpoints included the red blood cell(RBC)units transfused and adverse effects.In the placebo-controlled period,Hb concentrations in patients treated with thalidomide achieved a median elevation of 14.0(range,2.5 to 37.5)g/L,whereas Hb in patients treated with placebo did not significantly change.Within the 12 weeks,the mean RBC transfusion volume for patients treated with thalidomide and placebo was 5.4±5.0 U and 10.3±6.4 U,respectively(P<0.001).Adverse events of drowsiness,dizziness,fatigue,pyrexia,sore throat,and rash were more common with thalidomide than placebo.In the extension phase,treatment with thalidomide for 24 weeks resulted in a sustainable increase in Hb concentrations which reached 104.9±19.0 g/L,without blood transfusion.Significant increase in Hb concentration and reduction in RBC transfusions were associated with nonβ0/β0 and HBS1L-MYB(rs9399137 C/T,C/C;rs4895441 A/G,G/G)genotypes.These results demonstrated that thalidomide is effective in patients with TDT.展开更多
Inflammatory demyelinating diseases(IDD)of the central nervous system(CNS)occur throughout the world and are the leading cause of nontraumatic neurological disability in young adults.[1]Early diagnosis of IDD allows p...Inflammatory demyelinating diseases(IDD)of the central nervous system(CNS)occur throughout the world and are the leading cause of nontraumatic neurological disability in young adults.[1]Early diagnosis of IDD allows prompt immunotherapies that minimize relapse,disabilities and mortality.[2]Multiple sclerosis(MS)is the most frequent IDD in adults.The histological characteristics of chronic MS lesions are well known to neuropathologists;however,diagnosing an inflammatory demyelinating process in biopsy may be challenging since the specimens are frequently small and often represent only parts of a larger lesion.[3]Furthermore,the histopathological hallmarks of acute MS differ fundamentally from the well-known characteristics of chronic demyelinated plaques observed in autopsies.[3]Kuhlmann et al[4]recently proposed a new histological classification system for MS lesions.Whether this new classification system corresponds to clinical manifestations and thus reflects the temporal multiplicity of MS and whether it can be applied to all IDD cases remain to be further studied.展开更多
We present a sketch-based rotation editing system for enriching rotational motion in keyframe animations. Given a set of keyframe orientations of a rigid object, the user first edits its angular velocity trajectory by...We present a sketch-based rotation editing system for enriching rotational motion in keyframe animations. Given a set of keyframe orientations of a rigid object, the user first edits its angular velocity trajectory by sketching curves, and then the system computes the altered rotational motion by solving a variational curve fitting problem. The solved rotational motion not only satisfies the orientation constraints at the keyframes, but also fits well the user-specified angular velocity trajectory. Our system is simple and easy to use. We demonstrate its usefulness by adding interesting and realistic rotational details to several keyframe animations.展开更多
基金supported by the[Natural Science Foundation Project of Shandong Province#1]under Grant[ZR2019MEE033][Fundamental Research Funds for the central Universities#2]under Grant[19CX02035A].
文摘Catalytic cracking is the main method to lighten heavy crude oil,this process can produce high quality oil products such as gasoline and diesel,but also produces a large amount of fluid catalytic cracking slurry(FCCS).The catalyst particles in FCCS seriously restrict the secondary processing of FCCS and need to be removed,and the properties of Fccs is an important factor that affects the removal efficiency of the catalyst particles.Based on the"effective contact point"model proposed by the research group,this study further proposed the"electrostatic separation efficiency calculation"model.In this model,since Fccs has a uniform distribution of catalyst particles,the ratio of the number of catalyst particles can be expressed as the ratio of area to achieve the calculation of separation efficiency.Then the catalyst removal efficiency under different viscosity was analyzed,thus verifying the feasibility of this model.The effects of temperature and mass ratio of four components on the viscosity of FccS were investigated respectively,then the effects of temperature and four components'mass ratio on the electrostatic sep-aration can be directly converted into the effect of viscosity on the electrostatic separation efficiency.All the results show the electrostatic separation efficiency decreases with increasing viscosity,and the best separationtemperatureis120℃.
文摘AIM:Tocomparetheeffectsandcomplicationsof direc cyclopexy (DC) versus vitrectomy, endophotocoagulation, and gas/silicone oil endotamponade (VEE) treatment in patients with cyclodialysis and persistent hypotony.METHODS: This is a prospective, comparative, non-randomized clinical trial which includes 52 patients with cyclodialysis and persisting hypotony. Fifty-two patients suffering from cyclodialysis and persistent hypotony in one eye were divided into 2 groups (groups DC and VEE) and treated, respectively, with direct cyclopexy or vitrectomy, endophotocoagulation, and gas/silicone oil endotamponade. The patients were followed up for 12 months. Assessments included best corrected visual acuity (BCVA), intraocular pressure (IOP), anterior chamber depth (ACD), anterior chamber volume (ACV) and subjective rating of the pain caused by the treatments.RESULTS: After a follow-up of 12 months, significan improvement was seen in postoperative mean BCVA, IOP, ACD and ACV in both treatment groups (which were not significantly different from each other). The success rates for the treatments were not significantly different (DC: 50.0% vs VEE: 62.5% , P =0.383). Postoperative morbidity of cataract and subjective pain rating were significantly higher in the VEE group vs the DC group (P =0.003 and P <0.001 respectively).CONCLUSION: DC and VEE were effective surgica procedures in treating patients with cyclodialysis and persistent hypotony. Patients had better tolerance to DC treatment and VEE was more likely lead to cataract complications. Taking into consideration the ease of the operation, success rate, and patient comfort, DC treatment seems preferable to VEE treatment in patients with simple cyclodialysis. While VEE has the advantage of treating patients with cyclodialysis combined with vitreous hemorrhage.
文摘● AIM: To investigate the retinal photoreceptor differentiation potential of human orbital adipose tissue-derived stem cells(ADSCs) generated by enzyme(EN) and explant(EX) culture methods.● METHODS: We investigated potentials of human orbital ADSCs to differentiate into photoreceptors through EN and EX culture methods. EN and EX orbital ADSCs were obtained from the same donor during rehabilitative orbital decompression, and then were subject to a 3-step induction using Noggin, DKK-1, IGF-1 and b-FGF at different time points for 38 d. Stem cell, eye-field and photoreceptor-related gene and protein markers were measured by reverse transcription-polymerase chain reaction(RT-PCR) and immunofluorescent(IMF) staining. ● RESULTS: Both EX and EN orbital ADSCs expressed CD133, a marker of cell differentiation. Moreover, PAX6 and rhodopsin, markers of the retinal progenitor cells, were detected from EX and EN orbital ADSCs. In EX orbital ADSCs, PAX6 m RNA was detected on the 17^(th) day and then the rhodopsin m RNA was detected on the 24^(th) day. In contrast, the EN orbital ADSCs expressed PAX6 and rhodopsin m RNA on the 31^(st) day. EX orbital ADSCs expressed rhodopsin protein on the 24^(th) day, while EN orbital ADSCs expressed rhodopsin protein on the 31^(st) day.● CONCLUSION: Orbital ADSCs isolated by direct explants culture show earlier and stronger expressions of markers towards eye field and retinal photoreceptor differentiation than those generated by conventional EN method.
基金supported by grants from the National Natural Science Foundation of China(81270282,81070176,30600242,81170192,81200163)Wenzhou Science Technology Bureau Foundation(Y20100010)Education Foundation of Zhejiang Province(Y200906376)
文摘BACKGROUND: Retinoid X receptor(RXR) plays a central role in the regulation of intracellular receptor signaling pathways. The activation of RXR has protective effect on H2O2-induced apoptosis of H9c2 ventricular cells in rats. But the protective effect and mechanism of activating RXR in cardiomyocytes against hypoxia/reoxygenation(H/R)-induced oxidative iniury are still unclear.METHODS: The model of H/R injury was established through hypoxia for 2 hours and reoxygenation for 4 hours in H9c2 cardiomyocytes of rats. 9-cis-retinoic acid(9-cis RA) was obtained as an RXR agonist, and HX531 as an RXR antagonist. Cultured cardiomyocytes were randomly divided into four groups: sham group, H/R group, H/R+9-cis RA-pretreated group(100 nmol/L 9-cis RA), and H/R+9-cis RA+HX531-pretreated group(2.5 μmol/L HX531). The cell viability was measured by MTT, apoptosis rate of cardiomyocytes by flow cytometry analysis, and mitochondrial membrane potential(ΔΨm) by JC-1 fluorescent probe, and protein expressions of Bcl-2, Bax and cleaved caspase-9 with Western blotting. All measurement data were expressed as mean±standard deviation, and analyzed using one-way ANOVA and the Dunnett test. Differences were considered signif icant when P was <0.05.RESULTS: Pretreatment with RXR agonist enhanced cell viability, reduced apoptosis ratio, and stabled ΔΨm. Dot blotting experiments showed that under H/R stress conditions, Bcl-2 protein level decreased, while Bax and cleaved caspase-9 were increased. 9-cis RA administration before H/R stress prevented these effects, but the protective effects of activating RXR on cardiomyocytes against H/R induced oxidative injury were abolished when pretreated with RXR pan-antagonist HX531.CONCLUSION: The activation of RXR has protective effects against H/R injury in H9c2 cardiomyocytes of rats through attenuating signaling pathway of mitochondria apoptosis.
基金supported by the Independent Topic Project of China Academy of Chinese Medical Sciences(No.ZZ12007)the National Natural Science Foundation of China(No.81473780).
文摘Backgroud:The electroacupuncture of auricular concha area(EA-ACR)proved to have a good regulatory effect on insomnia and the release of melatonin,which is most closely related to sleep.However,there is little research to demonstrate the relationship between EA-ACR and melatonin.The objective of present study is to observe the effects of EA-ACR on the circadian rhythm of melatonin secretion in p-chlorophenylalanine(PCPA)-induced insomnia rats.Methods:The study divided 36 male Sprague-Dawley rats randomly into three groups:12 in blank group,12 in model group,and 12 in EA-ACR group.By using PCPA intraperitoneal injection for 2 days,insomnia model was established.EA-ACR group was treated for 28 days.We observed the effects of EA-ACR on the rats'behavioral changes,open-field test and weight during the experiment.After the experiment,the rat tail and heart blood were collected,and the melatonin was detected by enzyme-linked immunosorbent assay.Results:Compared with the blank group,28–30 hours after PCPA intraperitoneal injection,the circadian rhythm of sleep and the total sleep time of model rats decreased.Compared with the model group,electrical stimulation in the ear concha region could effectively reverse the behavioral changes of rats,and increase the trend of weight growth(P=0.010),the crossing score(P=0.014),vertical motion score(P=0.015)and the level of plasma melatonin secretion(P=0.020)with the trend showing a rhythmic pattern(0–60 minutes upward,60–120 minutes downward,120–150 minutes upward).Conclusion:EA-ACR can improve the quality of life(weight),ameliorate exploring irritable and anxiety behaviors in PCPA-induced insomnia rats.The underlying effects of EA-ACR may be excited on the circadian rhythm of melatonin secretion in PCPA-induced insomnia rats.
基金Project partially supported by the National Key R&D Program of of China(Grant No.2016YFA0301801)the National Natural Science Foundation of China(Grant Nos.61521001 and 61571219)PAPD,Dengfeng Project B of Nanjing University.
文摘An impedance matched parametric amplifier(IMPA)with Josephson junctions is fabricated and characterized.A hybrid structure containing coplanar and strip structures is implemented to realize an impedance taper line and a plate capacitor in an LC nonlinear resonator based on Josephson junctions.The upper plate of the capacitor is isolated with SiNx without grounding as well as the strips.Such easily-prepared designs greatly reduce the requirements for lithography alignment and precision,which makes the fabrication process more reliable.The experimental results show that in such IMPA a gain higher than 25 dB with a bandwidth of about 100 MHz can be obtained.This broadband amplifier operates close to the quantum limit.By adjusting the working point,a higher bandwidth of about 400 MHz can be obtained with a gain of about 17 dB.
基金Project partially supported by the National Key R&D Program of China(Grant No.2016YFA0301801)the National Natural Science Foundation of China(Grant Nos.11474154 and 61521001)+1 种基金PAPD,Dengfeng Project B of Nanjing Universitythe Fundamental Research Funds for the Central Universities,China(Grant No.14380134)。
文摘We design and fabricateλ/2 coplanar waveguide NbN resonators,the thickness and length of which are only several nanometers and hundred microns,respectively.The quality factor of such compact resonators can reach up to 7.5×10~4 at single photon power level at 30 m K with the resonance frequency around 6.835 GHz.In order to tune the resonant frequency,the resonator is terminated to the ground with a dc-SQUID.By tuning the magnetic flux in the dc-SQUID,the effective inductance of the dc-SQUID is varied,which leads to the change in the resonant frequency of the resonator.The tunability range is more than 30 MHz and the quality factor is about 3×10~3.These compact and tunable NbN resonators have potential applications in the quantum information processing,such as in the precision measurement,coupling and/or reading out the quantum states of qubits.
基金2020 clinical research innovation project of Dongzhimen Hospital,Beijing university of Chinese medicine(No.DZMKJCX-2020-002)。
文摘Insomnia has become one of the common clinical diseases.In recent years,it has been found that patients with insomnia of Yang deficiency type are more and more common,and the trend of rejuvenation gradually appears,which seriously affects the normal progress of people's life,work and study.Clinical treatment through syndrome differentiation,mainly from the heart,spleen,kidney three viscera yang deficiency,on the basis of the warm yang method to add Qianzhen tranquilizing products,and pay attention to multiple organs as diseases,add moisture,blood The qi thing,while not forgetting the psychological counseling,adjusting the body and mind,and achieving a certain clinical effect,can provide ideas for the treatment of this disease.
基金Supported by Horizontal Project of Shanghai Tenth People’s Hospital,No.DS05!06!22016 and No.DS05!06!22017.
文摘BACKGROUND Due to the complexity and numerous comorbidities associated with Crohn’s disease(CD),the incidence of postoperative complications is high,significantly impacting the recovery and prognosis of patients.Consequently,additional stu-dies are required to precisely predict short-term major complications following intestinal resection(IR),aiding surgical decision-making and optimizing patient care.AIM To construct novel models based on machine learning(ML)to predict short-term major postoperative complications in patients with CD following IR.METHODS A retrospective analysis was performed on clinical data derived from a patient cohort that underwent IR for CD from January 2017 to December 2022.The study participants were randomly allocated to either a training cohort or a validation cohort.The logistic regression and random forest(RF)were applied to construct models in the training cohort,with model discrimination evaluated using the area under the curves(AUC).The validation cohort assessed the performance of the constructed models.RESULTS Out of the 259 patients encompassed in the study,5.0%encountered major postoperative complications(Clavien-Dindo≥III)within 30 d following IR for CD.The AUC for the logistic model was 0.916,significantly lower than the AUC of 0.965 for the RF model.The logistic model incorporated a preoperative CD activity index(CDAI)of≥220,a diminished preoperative serum albumin level,conversion to laparotomy surgery,and an extended operation time.A nomogram for the logistic model was plotted.Except for the surgical approach,the other three variables ranked among the top four important variables in the novel ML model.CONCLUSION Both the nomogram and RF exhibited good performance in predicting short-term major postoperative complic-ations in patients with CD,with the RF model showing more superiority.A preoperative CDAI of≥220,a di-minished preoperative serum albumin level,and an extended operation time might be the most crucial variables.The findings of this study can assist clinicians in identifying patients at a higher risk for complications and offering personalized perioperative management to enhance patient outcomes.
基金This work was supported by grants from the Capital Health Research and Development of Special Program(No.2014-2-2013)the Beijing Excellent Talent Training Project Grant(No.201600026833ZK07)+1 种基金the National Science-Technology Support Plan(No.2014BAI04B02)the Project of Beijing Municipal Health Commission(No.PXM 2019_026283_000002).
文摘Background:Mutations in the isocitrate dehydrogenase 1(IDH1)and IDH2 genes are important for both the integrated diagnosis and the prognosis of diffuse gliomas.The p.R132H mutation of IDH1 is the most frequently observed IDH mutation,while IDH2 mutations were relatively rarely studied.The aim of the study was to determine the pathological and genetic characteristics of lowergrade gliomas that carry IDH2 mutations.Methods:Data from 238 adult patients with lower-grade gliomas were retrospectively analyzed.The status of IDH1/2 gene mutations,telomerase reverse transcriptase(TERT)promoter mutations,O^6-methylguanine-DNA-methyltransferase(MGMT)promoter methylation,1p/19q co-deletion and the expressions of IDH1 R132H,alpha-thalassemia X-linked mental retardation,and p53 were evaluated.Progression-free survival(PFS)and overall survival(OS)were calculated via Kaplan-Meier estimation using the log-rank test.Results:Totally,71%(169/238)of patients were positive for IDH mutations,including 12 patients harboring mutations in IDH2.Among the 12 patients with IDH2 mutations,ten patients harbored the R172K mutation,one patient harbored the R172S mutation and one harbored the R172W mutation.Of these,11 tumors occurred in the frontal lobe and showed morphology typical of oligodendroglioma.The proportion of grade II tumors was higher than that of grade III tumors in IDH2 mutant-gliomas.IDH2 mutations were frequently associated with TERT promoter mutations,1p/19q co-deletion and MGMT promoter methylation.IDH2 mutations were associated with better outcomes compared with IDH wild-type gliomas(P<0.05).However,the PFS and OS did not differ from that of IDH1 mutant patients(P=0.95 and P=0.60,respectively).Conclusions:IDH2 mutations are more frequent in oligodendrogliomas and associated with a better prognosis.IDH2 mutations may segregate in distinct clinico-pathological and genetic subtypes of gliomas,and therefore may merit routine investigation.
基金This work is partially supported by the National Natural Science Foundation of China under Grant Nos. 61272305, 61303136, 61272392, and 61322204, and the National Program for Special Support of Eminent Professionals of China.
文摘图象 compositing 广泛地被用来从分开的来源图象把视觉元素合为一幅单个图象。尽管最近的图象 compositing 技术能够完成从不同来源的视觉元素的光滑的相配，他们中的大多数含蓄地假设来源图象在一样的观点被拿。在这份报纸，我们在场从多重来源的 compositing 小说图象目标的一条途径想象哪个有不同观点。我们的关键想法是为来源图象目标的有意义的部件构造 3D 代理，并且使用这些 3D 部件代理在一样的观点一起弄歪并且无缝地合并部件。认识到这个想法，我们介绍一个并列框架的基于的单个看法的照相机刻度算法处理图象对象的一般类型，得到长方体的一个结构知道的长方体优化算法正确结构关系地为图象对象部件代理，并且最后， 3D 代理转变指导了图象变弯缝对象部件的算法。我们进一步基于这条 compositing 途径描述一个新奇应用程序自动地综合从一套模范的很多图象对象。试验性的结果证明我们的 compositing 途径能被用于许多图象目标，例如椅子，杯，灯，和机器人，并且合成申请能从模范的一个小集合与重要形状和风格变化创造新奇图象目标。
基金This work was supported by the National Key Research and Development Program of China(No.2020YFA0803300)the CAMS Innovation Fund for Medical Sciences(CIFMS,Nos.2021-RC310-003,2020-RC310-002)+6 种基金CAMS Initiative for Innovative Medicine(2021-1-I2M-012)the Key Project of the National Natural Science Foundation of China(81830093)Guangxi Natural Science Foundation(2020GXNSFAA159097)the Funding for Guangxi Thalassemia Prevention Capacity Improvement Project,the Overseas Expertise Introduction Project for Discipline Innovation(111 Project,B17029)the Double First-Class Project(WF510162602)of Shanghai Jiao Tong UniversityShanghai Collaborative Innovation Program on Regenerative Medicine and Stem Cell Research(2019CXJQ01)Shanghai Guangci Translational Medical Research Development Foundation.
文摘Thalidomide inducesγ-globin expression in erythroid progenitor cells,but its efficacy on patients with transfusion-dependentβ-thalassemia(TDT)remains unclear.In this phase 2,multi-center,randomized,double-blind clinical trial,we aimed to determine the safety and efficacy of thalidomide in TDT patients.A hundred patients of 14 years or older were randomly assigned to receive placebo or thalidomide for 12 weeks,followed by an extension phase of at least 36 weeks.The primary endpoint was the change of hemoglobin(Hb)level in the patients.The secondary endpoints included the red blood cell(RBC)units transfused and adverse effects.In the placebo-controlled period,Hb concentrations in patients treated with thalidomide achieved a median elevation of 14.0(range,2.5 to 37.5)g/L,whereas Hb in patients treated with placebo did not significantly change.Within the 12 weeks,the mean RBC transfusion volume for patients treated with thalidomide and placebo was 5.4±5.0 U and 10.3±6.4 U,respectively(P<0.001).Adverse events of drowsiness,dizziness,fatigue,pyrexia,sore throat,and rash were more common with thalidomide than placebo.In the extension phase,treatment with thalidomide for 24 weeks resulted in a sustainable increase in Hb concentrations which reached 104.9±19.0 g/L,without blood transfusion.Significant increase in Hb concentration and reduction in RBC transfusions were associated with nonβ0/β0 and HBS1L-MYB(rs9399137 C/T,C/C;rs4895441 A/G,G/G)genotypes.These results demonstrated that thalidomide is effective in patients with TDT.
基金The work was supported by the National Key Research and Development Program of China under Grant No. 2017YFC0804401, the National Natural Science Foundation of China under Grant Nos. 61472370, 61672469, 61379079, 61322204, and 61502433, the Natural Science Foundation of Henan Province of China under Grant No. 162300410262, and the Key Research Projects of Henan Higher Education Institutions of China under Grant No. 18A413002.
文摘Inflammatory demyelinating diseases(IDD)of the central nervous system(CNS)occur throughout the world and are the leading cause of nontraumatic neurological disability in young adults.[1]Early diagnosis of IDD allows prompt immunotherapies that minimize relapse,disabilities and mortality.[2]Multiple sclerosis(MS)is the most frequent IDD in adults.The histological characteristics of chronic MS lesions are well known to neuropathologists;however,diagnosing an inflammatory demyelinating process in biopsy may be challenging since the specimens are frequently small and often represent only parts of a larger lesion.[3]Furthermore,the histopathological hallmarks of acute MS differ fundamentally from the well-known characteristics of chronic demyelinated plaques observed in autopsies.[3]Kuhlmann et al[4]recently proposed a new histological classification system for MS lesions.Whether this new classification system corresponds to clinical manifestations and thus reflects the temporal multiplicity of MS and whether it can be applied to all IDD cases remain to be further studied.
基金supported by the National Natural Science Foundation of China (No. 61003145)the Fundamental Research Fundsfor the Central Universities, China (No. 2009QNA5018)
文摘We present a sketch-based rotation editing system for enriching rotational motion in keyframe animations. Given a set of keyframe orientations of a rigid object, the user first edits its angular velocity trajectory by sketching curves, and then the system computes the altered rotational motion by solving a variational curve fitting problem. The solved rotational motion not only satisfies the orientation constraints at the keyframes, but also fits well the user-specified angular velocity trajectory. Our system is simple and easy to use. We demonstrate its usefulness by adding interesting and realistic rotational details to several keyframe animations.
