A survivin siRNA expression vector was transfected into glioma U-87MG cells and these cells were then treated with paclitaxel.The results showed that survivin-specific siRNA combined with paclitaxel treatment synergis...A survivin siRNA expression vector was transfected into glioma U-87MG cells and these cells were then treated with paclitaxel.The results showed that survivin-specific siRNA combined with paclitaxel treatment synergistically inhibited glioma U-87MG cell proliferation and promoted apoptosis.This treatment also inhibited the expression of the cell cycle regulatory proteins,survivin,cyclinD1,c-Myc and CDK4 and enhanced the sensitivity of U-87MG cells to paclitaxel.展开更多
A survivin small interfering RNA sequence specific for a human and mouse homogenous sequence was constructed.Survivin small interfering RNA could significantly inhibit glioma cell proliferation and induce apoptosis wh...A survivin small interfering RNA sequence specific for a human and mouse homogenous sequence was constructed.Survivin small interfering RNA could significantly inhibit glioma cell proliferation and induce apoptosis when it was transfected into either a human glioma cell line U251 or rat glioma C6 cells in vitro.In addition,treatment of rat orthotopic glioma models with survivin small interfering demonstrated the inhibition of glioma growth in vivo.Our experimental findings suggest that the use of RNA interference techniques to target the survivin sequence may be useful in the treatment of glioma.展开更多
基金supported by the Natural Science Foundation of Jilin Province,No.2011146,2012150and201215100the Natural Science Foundation of Jilin Technology Division,No.201133139and201032235
文摘A survivin siRNA expression vector was transfected into glioma U-87MG cells and these cells were then treated with paclitaxel.The results showed that survivin-specific siRNA combined with paclitaxel treatment synergistically inhibited glioma U-87MG cell proliferation and promoted apoptosis.This treatment also inhibited the expression of the cell cycle regulatory proteins,survivin,cyclinD1,c-Myc and CDK4 and enhanced the sensitivity of U-87MG cells to paclitaxel.
基金supported by the Natural Science Foundation of Jilin Province,No.2011146,2012150the Natural Science Foundation of Jilin Technology Division,No.201133139 and 201032235
文摘A survivin small interfering RNA sequence specific for a human and mouse homogenous sequence was constructed.Survivin small interfering RNA could significantly inhibit glioma cell proliferation and induce apoptosis when it was transfected into either a human glioma cell line U251 or rat glioma C6 cells in vitro.In addition,treatment of rat orthotopic glioma models with survivin small interfering demonstrated the inhibition of glioma growth in vivo.Our experimental findings suggest that the use of RNA interference techniques to target the survivin sequence may be useful in the treatment of glioma.
