This paper uses the theory of planar dynamic systems and the knowledge of reaction-diffusion equations,and then studies the bounded traveling wave solution of the generalized Boussinesq equation affected by dissipatio...This paper uses the theory of planar dynamic systems and the knowledge of reaction-diffusion equations,and then studies the bounded traveling wave solution of the generalized Boussinesq equation affected by dissipation and the influence of dissipation on solitary waves.The dynamic system corresponding to the traveling wave solution of the equation is qualitatively analyzed in detail.The influence of the dissipation coefficient on the solution behavior of the bounded traveling wave is studied,and the critical values that can describe the magnitude of the dissipation effect are,respectively,found for the two cases of b_3<0 and b_3>0 in the equation.The results show that,when the dissipation effect is significant(i.e.,r is greater than the critical value in a certain situation),the traveling wave solution to the generalized Boussinesq equation appears as a kink-shaped solitary wave solution;when the dissipation effect is small(i.e.,r is smaller than the critical value in a certain situation),the traveling wave solution to the equation appears as the oscillation attenuation solution.By using the hypothesis undetermined method,all possible solitary wave solutions to the equation when there is no dissipation effect(i.e.,r=0)and the partial kink-shaped solitary wave solution when the dissipation effect is significant are obtained;in particular,when the dissipation effect is small,an approximate solution of the oscillation attenuation solution can be achieved.This paper is further based on the idea of the homogenization principles.By establishing an integral equation reflecting the relationship between the approximate solution of the oscillation attenuation solution and the exact solution obtained in the paper,and by investigating the asymptotic behavior of the solution at infinity,the error estimate between the approximate solution of the oscillation attenuation solution and the exact solution is obtained,which is an infinitesimal amount that decays exponentially.The influence of the dissipation coefficient on the amplitude,frequency,period,and energy of the bounded traveling wave solution of the equation is also discussed.展开更多
Risk management is an important aspect of financial research because correlations among financial data are essential in evaluating portfolio risk.Among various correlations,spatiotemporal correlations involve economic...Risk management is an important aspect of financial research because correlations among financial data are essential in evaluating portfolio risk.Among various correlations,spatiotemporal correlations involve economic entity attributes and are interrelated in space and time.Such correlations have therefore drawn increasing attention in financial risk management.However,classical correlation measurements are typically based on either time series correlations or spatial dependence;they cannot be directly applied to financial data with spatiotemporal correlations.The spatiotemporal correlation coefficient model with adaptive weight proposed in this paper can(1)address the absolute quantity,dynamic quantity,and dynamic development of financial data and(2)be used for risk grading,financial risk evaluation,and portfolio management.To verify the validity and superiority of this model,cluster analysis results and portfolio performance are compared with a classical model with time series correlation or spatial correlation,respectively.Empirical findings show that the proposed coefficient is highly effective and convenient compared to others.Overall,our method provides a highly efficient financial risk management method with valuable implications for investors and financial institutions.展开更多
In this study,phosphoenolpyruvate and erythrose-4-phosphate are efficiently supplied by collaborative design of Embden-Meyerhof-Parnas(EMP)pathway and pentose phosphate(PP)pathway in Escherichia coli,thus increasing t...In this study,phosphoenolpyruvate and erythrose-4-phosphate are efficiently supplied by collaborative design of Embden-Meyerhof-Parnas(EMP)pathway and pentose phosphate(PP)pathway in Escherichia coli,thus increasing the L-tryptophan production.Firstly,the effects of disrupting EMP pathway on L-tryptophan production were studied,and the results indicated that the strain with deletion of phosphofructokinase A(i.e.,E.coli JW-5ΔpfkA)produced 23.4±2.1 g/L of L-tryptophan production.However,deletion of phosphofructokinase A and glucosephosphate isomerase is not conducive to glucose consumption and cell growth,especially deletion of glucosephosphate isomerase.Next,the carbon flux in PP pathway was enhanced by introduction of the desensitized glucose-6-phosphate dehydrogenase(zwf)and 6-phosphogluconate dehydrogenase(gnd)and thus increasing the L-tryptophan production(i.e.,26.5±3.2 g/L vs.21.7±1.3 g/L)without obviously changing the cell growth(i.e.,0.41 h^(-1) vs.0.44 h^(-1))as compared with the original strain JW-5.Finally,the effects of co-modifying EMP pathway and PP pathway on L-tryptophan production were investigated.It was found that the strain with deletion of phosphofructokinase A as well as introduction of the desensitized zwf and gnd(i.e.,E.coli JW-5 zwf243 gnd361ΔpfkA)produced 31.9±2.7 g/L of L-tryptophan,which was 47.0%higher than that of strain JW-5.In addition,the glucose consumption rate of strain JW-5 zwf243 gnd361ΔpfkA was obviously increased despite of the bad cell growth as compared with strain JW-5.The results of this study have important reference value for the following application of metabolic engineering to improve aromatic amino acids producing strains.展开更多
Background: The physiological and operative severity score for the enumeration of mortality and morbidity (POSSUM) is a scoring system previously validated in vascular and general surgical patients. The Portsmouth phy...Background: The physiological and operative severity score for the enumeration of mortality and morbidity (POSSUM) is a scoring system previously validated in vascular and general surgical patients. The Portsmouth physiological and operative severity score for the enumeration of mortality and morbidity (P-POSSUM) is a reliable tool derived from POSSUM to improve the prediction of mortality. In this paper, we utilized both models to determine the most suitable and feasible one that could be utilized in orthopedic surgery in China mainland. Methods: In this retrospective study, patients’ files were extracted randomly from the medical records department of the First Hospital Affiliated with Dalian Medical University for those underwent hip replacements between 1999 and 2006. The mortality and morbidity rates were predicted by P-POSSUM and POSSUM, respectively. A comparative analysis was performed between the observed and the predicted values as well as the Observed/Expected ratio (O:E). Results: A total of 206 patients were selected for this study after fulfilling the inclusion and exclusion criteria. The predicted mortality by P-POSSUM were not significantly different from the observed values (X2 = 2.10, P = 0.552). POSSUM appeared to be better with the observed morbidity (X2 = 2.766, P = 0.598), but had overestimated mortality. Conclusion: The POSSUM morbidity equation satisfactorily estimated the risk of postoperative complications, and the P-POSSUM mortality equation appeared to stratify the risk of death more accurate than the POSSUM.展开更多
Reducing drag during take-off and nominal(cruise)conditions is a problem of fundamental importance in aeronautical engineering.Existing studies have demonstrated that v-shaped symmetrical riblets can effectively be us...Reducing drag during take-off and nominal(cruise)conditions is a problem of fundamental importance in aeronautical engineering.Existing studies have demonstrated that v-shaped symmetrical riblets can effectively be used for turbulence control,with those with dimensionless depth h+=15 and dimensionless width s+=15 having the best drag reduction effect.In the present study,experimental tests have been conducted considering two models of the same size,one with smooth surface,the other with v-shaped riblets of the h+=15 and s+=15 type.The results show that for an angle of attack in the 8°~20°range(take-off stage),the maximum lift coefficient can be increased by 22%.For angle of attack between 8°and 14°,a drag reduction effect can be produced using riblets,which increases with the Reynolds number,leading to a decrease in the drag coefficient maximum of 36%.Flow visualization experiments have been carried out by means of Laser Induced fluorescence.展开更多
This study investigates the predictability of a fixed uncertainty index(UI)for realized variances(volatility)in the international stock markets from a high-frequency perspective.We construct a composite UI based on th...This study investigates the predictability of a fixed uncertainty index(UI)for realized variances(volatility)in the international stock markets from a high-frequency perspective.We construct a composite UI based on the scaled principal component analysis(s-PCA)method and demonstrate that it exhibits significant in-and out-of-sample predictabilities for realized variances in global stock markets.This predictive power is more powerful than those of two commonly employed competing methods,namely,PCA and the partial least squares(PLS)methods.The result is robust in several checks.Further,we explain that s-PCA outperforms other dimension-reduction methods since it can effectively increase the impacts of strong predictors and decrease those of weak factors.The implications of this research are significant for investors who allocate assets globally.展开更多
The material composition of geological samples is very complicated. Generally, the method for accurately determining trace elements in geological samples needs to digest the solid samples into liquid state, which is c...The material composition of geological samples is very complicated. Generally, the method for accurately determining trace elements in geological samples needs to digest the solid samples into liquid state, which is convenient for inductively coupled plasma mass spectrometry. The previous digestion method takes a long time and the operation process is complicated. The Ultra CLAVE microwave digestion instrument was used to digest the geological samples. The types and dosages of the acid used for digestion and the optimal conditions for instrumental testing were published. The results show that this method has short processing time, small data error, safer operation and good digestion effect. This method is suitable for geological sample analysis.展开更多
Stone coal is a special resource, because it is associated with a variety of rare metal elements. The elemental geochemical characteristics of stone coal can provide useful information for the study of its formation a...Stone coal is a special resource, because it is associated with a variety of rare metal elements. The elemental geochemical characteristics of stone coal can provide useful information for the study of its formation and evolution history. The content of rare earth elements in stone coal has been the focus of attention. Stone coal is widely distributed in southern Shaanxi province of China. In order to study the content and distribution of rare earth elements in stone coal, and provide a plan for the comprehensive development and utilization of stone coal, this study tested the rare earth elements content of stone coal, parting and coal ash samples, respectively, compared the content of rare earth elements in stone coal, parting, coal ash with the mean value of China coal, the mean value of world coal and the abundance value of upper crust, and discussed the features of rare earth elements content in different types of samples in southern Shaanxi province of China. The average contents of rare earth elements in the samples of stone coal, parting and coal ash were calculated, and the enrichment coefficients of the samples with the mean value of China coal, the mean value of world coal and the abundance value of upper crust were listed. The distribution rules and characteristics of the contents of rare earth elements in different types of samples in southern Shaanxi province of China were summarized.展开更多
Increasing urban pluvial flood disasters due to climate change and rapid urbanisation have been a great challenge worldwide.Timely and effective emergency evacuation is important for reducing casualties and losses.Thi...Increasing urban pluvial flood disasters due to climate change and rapid urbanisation have been a great challenge worldwide.Timely and effective emergency evacuation is important for reducing casualties and losses.This has become a bottleneck for emergency management.This study aimed to develop a commonly used Agent-Based Mode(ABM)for pluvial flood emergency evacuation at the city scale,exploring the cascading impacts of pluvial flooding on human behaviour and emergency evacuation.The July 2021 pluvial flood event in Zhengzhou,Henan Province,claiming 380 lives and 40.9 billion yuan in direct losses,was selected as this case study.A raster-based hydraulic model(ECNU Flood-Urban)was used to predict flood inundation(extent and depth)during an event in Zhengzhou’s centre.Moreover,a comparative analysis of emergency evacuations was conducted before and after the pluvial flood event.The results showed that crowd behaviour plays an important role in an emergency evacuation,and extensive flooding leads to an 11-83%reduction in the number of evacuees.This study highlights the importance of risk education and contingency plans in emergency response.The ABM model developed in this study is proven to be effective and practical and will provide support for decision-making in urban flood emergency management.展开更多
SEL1L-mediated endoplasmic reticulum-associated degradation(ERAD)plays critical roles in controlling protein homeostasis by degrading misfolded or terminal unfolded proteins.However,it remains unclear how SEL1L regula...SEL1L-mediated endoplasmic reticulum-associated degradation(ERAD)plays critical roles in controlling protein homeostasis by degrading misfolded or terminal unfolded proteins.However,it remains unclear how SEL1L regulates peripheral T-cell survival and homeostasis.Herein,we found that SEL1L deficiency led to a greatly reduced frequency and number of mature T cells,which was further validated by adoptive transfer experiments or bone marrow chimera experiments,accompanied by the induction of multiple forms of cell death.Furthermore,SEL1L deficiency selectively disrupted naïve CD8+T-cell homeostasis,as indicated by the severe loss of the naïve T-cell subset but an increase in the memory T-cell subset.We also found that SEL1L deficiency fueled mTORC1/c-MYC activation and induced a metabolic shift,which was largely attributable to enhanced expression of the IL-15 receptorαandβchains.Mechanistically,single-cell transcriptomic profiling and biochemical analyses further revealed that Sel1l−/−CD8+T cells harbored excessive ER stress,particularly aberrant activation of the PERK-ATF4-CHOP-Bim pathway,which was alleviated by supplementing IL-7 or IL-15.Importantly,PERK inhibition greatly resolved the survival defects of Sel1l−/−CD8+T cells.In addition,IRE1αdeficiency decreased mTORC1 signaling in Sel1l−/−naïve CD8+T cells by downregulating the IL-15 receptorαchain.Altogether,these observations suggest that the ERAD adaptor molecule SEL1L acts as an important checkpoint for preserving the survival and homeostasis of peripheral T cells by regulating the PERK signaling cascade and IL-15 receptor-mediated mTORC1 axis.展开更多
For a conventional cascaded metasurface,the combination channel and each single channel are mutually dependent because the phase modulation of a cascaded metasurface is the sum of each single one.Here we propose a cas...For a conventional cascaded metasurface,the combination channel and each single channel are mutually dependent because the phase modulation of a cascaded metasurface is the sum of each single one.Here we propose a cascaded metasurface that can independently encode information into multiple channels.Based on the orientation degeneracy of anisotropic metasurfaces,each single metasurface can produce a quick-response(QR)image in the near field,governed by the Malus law,while the combined channel can produce a holographic image in the far field,governed by geometric phase.The independent and physically separated trichannel design makes information encryption safer.展开更多
This study focuses on the decreasing relevance of financial information associated with current financial reporting standards for intangible assets.We summarize and compare three approaches to improving financial repo...This study focuses on the decreasing relevance of financial information associated with current financial reporting standards for intangible assets.We summarize and compare three approaches to improving financial reporting standards for internally generated intangibles—the recognition approach,the fair value approach and the disclosure approach,among which we focus on the recognition approach.We investigate the impact of current International Accounting Standard 38 on the R&D capitalization policies of the high-tech industry,particularly among medical device firms in China.We conclude that the current recognition criteria are so stringent that they disincentivize firms from capitalizing their R&D investments.A large variation exists in capitalization timing within the medical device industry.Accordingly,we propose the milestone approach to revising financial reporting standards for intangible assets.We suggest that determining the capitalization criteria for intangibles based on the R&D cycle and capitalization timing should be moved forward.展开更多
CE-1 Lunar Microwave Sounder (CELMS) is the first passive microwave radiometer in the world to sound the surface of the Moon in the lunar orbit at altitude of 200 km. The scientific objective of CELMS is to obtain glo...CE-1 Lunar Microwave Sounder (CELMS) is the first passive microwave radiometer in the world to sound the surface of the Moon in the lunar orbit at altitude of 200 km. The scientific objective of CELMS is to obtain global brightness temperature (TB) of the Moon, to retrieve information on lunar regolith, and to evaluate the distribution of helium-3 on the Moon implanted by solar wind. Before launch of CELMS, a series of experiments were carried out in laboratories to test the performances of the systems, and to calibrate the responses between the input of TB and the output of voltage from the receivers. However, the thermal condition exposed to CELMS is more complicated in lunar orbit than on the Earth, which makes the temperatures of different parts of CELMS wave vary greatly, and the cosmic background is not very clean due to the pointing of cold space antenna to the direction of the satellite running, which brings uncertainties into data-processing of CELMS when the temperature of cold space is used as a calibrator. Furthermore, the lack of knowledge on the lunar ingredients and compositions, distributions of physical temperatures, and properties on lunar microwave radiation leads to difficulties in validating the measurements and retrievals of CELMS. By analyzing the results of ground experiments and the measurements of CELMS in-orbit, along with our knowledge of the properties of lunar surface, here we give algorithms on calibration and antenna pattern correction (APC) of CELMS. We also describe in detail the principle of microwave transfer among the elements of CELMS, and discuss the method on testing calibration parameters of the system. In addition, the theory and model on correction antenna pattern of CELMS are developed by comparing antenna temperatures by CELMS with those simulated by microwave radiative transfer models. The global distribution of TB is given and the features of TB are analyzed. Our results show rich information included in TB on the properties of lunar regolith, especially the thickness and dielectric constant, which are nearly directly reflected by the differences of TB at day and those at night.展开更多
This study presents the results obtained from magnetic susceptibility and heavy metal （Cu, Zn, Pb, and Cr） concentration measurements of soil profiles collected from arable land and urban parks in Baoshan District, ...This study presents the results obtained from magnetic susceptibility and heavy metal （Cu, Zn, Pb, and Cr） concentration measurements of soil profiles collected from arable land and urban parks in Baoshan District, an industrial district of Shanghai, China. The study focuses on the investigation of vertical variations in magnetic susceptibilities and heavy metal concentrations and on correlations between magnetic susceptibilities and heavy metal concentrations in soil profiles. The results demon- strate that magnetic enhancement in the surface layer of the soil profile is associated with increased heavy metal pollution. The enrichment factors （EF） and the Tomlinson Pollution Load Index （PLI-EF） are calculated for estimat- ing the level of heavy metal pollution of soil profiles in the study. The significant positive correlations between heavy metal contents, enrichment factors （EF）, Tomlinson pollution load index （PLI-CF）, modified Tomlinson pollution load index （PLI-EF）, and magnetic susceptibility （X） indicate that much of the heavy metal contamination in the study area is linked to combustion derived particulate emissions. The results confirm that the combined magnetic measurement and heavy metal concentration analysis could provide useful information for soil monitoring in urban environments. However, the use of magnetic technique to locate the heavy metal pollution boundary in the soil profile of this studied area should be confirmed by further geochemical analysis.展开更多
On 12 March 2020,the outbreak of coronavirus disease 2019(COVID-19)was declared a pandemic by the World Health Organization.As of 4 August 2020,more than 18 million confirmed infections had been reported globally.Most...On 12 March 2020,the outbreak of coronavirus disease 2019(COVID-19)was declared a pandemic by the World Health Organization.As of 4 August 2020,more than 18 million confirmed infections had been reported globally.Most patients have mild symptoms,but some patients develop respiratory failure which is the leading cause of death among COVID-19 patients.Endothelial cells with high levels of angiotensin-converting enzyme 2 expression are major participants and regulators of inflammatory reactions and coagulation.Accumulating evidence suggests that endothelial activation and dysfunction participate in COVID-19 pathogenesis by altering the integrity of vessel barrier,promoting pro-coagulative state,inducing endothelial inflammation,and even mediating leukocyte infiltration.This review describes the proposed cellular and molecular mechanisms of endothelial activation and dysfunction during COVID-19 emphasizing the principal mediators and therapeutic implications.展开更多
The global avalanche characteristics （the sum- of-squares indicator and the absolute indicator） measure the overall avalanche characteristics of a cryptographic Boolean function. Sung et al. （1999） gave the lower ...The global avalanche characteristics （the sum- of-squares indicator and the absolute indicator） measure the overall avalanche characteristics of a cryptographic Boolean function. Sung et al. （1999） gave the lower bound on the sum- of-squares indicator for a balanced Boolean function satisfy- ing the propagation criterion with respect to some vectors. In this paper, if balanced Boolean functions satisfy the propaga- tion criterion with respect to some vectors, we give three nec- essary and sufficient conditions on the auto-correlation distri- bution of these functions reaching the minimum the bound on the sum-of-squares indicator. And we also find all Boolean functions with 3-variable, 4-variable, and 5-variable reaching the minimum the bound on the sum-of-squares indicator.展开更多
Investigation on Lunar polar area is almost every lunar mission’s primary objective in recent years. The rationale behind it is that illumination and ice resources in this area can be potentially very helpful for con...Investigation on Lunar polar area is almost every lunar mission’s primary objective in recent years. The rationale behind it is that illumination and ice resources in this area can be potentially very helpful for constructing lunar human base. In this paper, we analyze microwave radiometric characteristics of the Moon by using the newly acquired Chang’E-1 Lunar Microwave Sounder (CELMS) data. Microwave brightness temperature at Lunar South Pole (LSP) is distributed regularly with a style of "ring-in-ring", decreasing from equator to pole. Regolith temperature gradient is bigger at lunar equator than at polar area. Brightness temperature diurnal difference decreases with observation frequency. Microwave brightness temperature distribution maps at LSP and Lunar North Pole (LNP) have been made based on the analysis. It is found that microwave brightness temperature becomes to synchronize with elevation beyond -85° latitude. This phenomenon is related to lightening condition and indicates temperature distribution at LSP. The brightness temperature anomaly cold points are potentially cold trap areas for water or ice while hot points imply plenty of illumination resources there.展开更多
Aims Both extreme drought and insect herbivores can suppress plant growth in grassland communities.However,most studies have examined extreme drought and insects in isolation,and there is reason to believe that insect...Aims Both extreme drought and insect herbivores can suppress plant growth in grassland communities.However,most studies have examined extreme drought and insects in isolation,and there is reason to believe that insects might alter the ability of grasslands to withstand drought.Unfortunately,few studies have tested the interactive effects of extreme drought and insect herbivores in grassland communities.Methods Here,we tested the drought–herbivore interactions using a manipulative experiment that factorially crossed extreme drought with the exclusion of insect herbivores in a temperate semiarid grassland in Inner Mongolia.Important Findings Our results demonstrated that both extreme drought and insect herbivores separately decreased total plant cover.When combined,insect herbivores reduced the impact of drought on total cover by increasing the relative abundance of drought-resistant dominant species.Our results highlight that the negative effect of extreme drought on total plant cover could be alleviated by maintaining robust insect herbivore communities.展开更多
基金Project supported by the National Natural Science Foundation of China(No.11471215)。
文摘This paper uses the theory of planar dynamic systems and the knowledge of reaction-diffusion equations,and then studies the bounded traveling wave solution of the generalized Boussinesq equation affected by dissipation and the influence of dissipation on solitary waves.The dynamic system corresponding to the traveling wave solution of the equation is qualitatively analyzed in detail.The influence of the dissipation coefficient on the solution behavior of the bounded traveling wave is studied,and the critical values that can describe the magnitude of the dissipation effect are,respectively,found for the two cases of b_3<0 and b_3>0 in the equation.The results show that,when the dissipation effect is significant(i.e.,r is greater than the critical value in a certain situation),the traveling wave solution to the generalized Boussinesq equation appears as a kink-shaped solitary wave solution;when the dissipation effect is small(i.e.,r is smaller than the critical value in a certain situation),the traveling wave solution to the equation appears as the oscillation attenuation solution.By using the hypothesis undetermined method,all possible solitary wave solutions to the equation when there is no dissipation effect(i.e.,r=0)and the partial kink-shaped solitary wave solution when the dissipation effect is significant are obtained;in particular,when the dissipation effect is small,an approximate solution of the oscillation attenuation solution can be achieved.This paper is further based on the idea of the homogenization principles.By establishing an integral equation reflecting the relationship between the approximate solution of the oscillation attenuation solution and the exact solution obtained in the paper,and by investigating the asymptotic behavior of the solution at infinity,the error estimate between the approximate solution of the oscillation attenuation solution and the exact solution is obtained,which is an infinitesimal amount that decays exponentially.The influence of the dissipation coefficient on the amplitude,frequency,period,and energy of the bounded traveling wave solution of the equation is also discussed.
基金supported by International(Regional)Cooperation and Exchange Project(71720107002)the National Natural Science Foundation of China(Nos.72161001 and 71963001)+2 种基金Guangxi Natural Science Fund(2018GXNSFBA050012)Key Research Base of Humanities and Social Sciences in Guangxi Universities Guangxi Development Research Strategy Institute(2021GDSIYB04,2022GDSIYB08)Project of Guangzhou Financial Service Innovation and Risk Management Research Base(No.PTJS202204).
文摘Risk management is an important aspect of financial research because correlations among financial data are essential in evaluating portfolio risk.Among various correlations,spatiotemporal correlations involve economic entity attributes and are interrelated in space and time.Such correlations have therefore drawn increasing attention in financial risk management.However,classical correlation measurements are typically based on either time series correlations or spatial dependence;they cannot be directly applied to financial data with spatiotemporal correlations.The spatiotemporal correlation coefficient model with adaptive weight proposed in this paper can(1)address the absolute quantity,dynamic quantity,and dynamic development of financial data and(2)be used for risk grading,financial risk evaluation,and portfolio management.To verify the validity and superiority of this model,cluster analysis results and portfolio performance are compared with a classical model with time series correlation or spatial correlation,respectively.Empirical findings show that the proposed coefficient is highly effective and convenient compared to others.Overall,our method provides a highly efficient financial risk management method with valuable implications for investors and financial institutions.
基金This work as financially supported by the National Key Research and Development Program of China(2021YFC2100900)the Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University(KLIB-KF 202004)Fundamental Research Funds for the Central Universities[No.JUSRP115A19].
文摘In this study,phosphoenolpyruvate and erythrose-4-phosphate are efficiently supplied by collaborative design of Embden-Meyerhof-Parnas(EMP)pathway and pentose phosphate(PP)pathway in Escherichia coli,thus increasing the L-tryptophan production.Firstly,the effects of disrupting EMP pathway on L-tryptophan production were studied,and the results indicated that the strain with deletion of phosphofructokinase A(i.e.,E.coli JW-5ΔpfkA)produced 23.4±2.1 g/L of L-tryptophan production.However,deletion of phosphofructokinase A and glucosephosphate isomerase is not conducive to glucose consumption and cell growth,especially deletion of glucosephosphate isomerase.Next,the carbon flux in PP pathway was enhanced by introduction of the desensitized glucose-6-phosphate dehydrogenase(zwf)and 6-phosphogluconate dehydrogenase(gnd)and thus increasing the L-tryptophan production(i.e.,26.5±3.2 g/L vs.21.7±1.3 g/L)without obviously changing the cell growth(i.e.,0.41 h^(-1) vs.0.44 h^(-1))as compared with the original strain JW-5.Finally,the effects of co-modifying EMP pathway and PP pathway on L-tryptophan production were investigated.It was found that the strain with deletion of phosphofructokinase A as well as introduction of the desensitized zwf and gnd(i.e.,E.coli JW-5 zwf243 gnd361ΔpfkA)produced 31.9±2.7 g/L of L-tryptophan,which was 47.0%higher than that of strain JW-5.In addition,the glucose consumption rate of strain JW-5 zwf243 gnd361ΔpfkA was obviously increased despite of the bad cell growth as compared with strain JW-5.The results of this study have important reference value for the following application of metabolic engineering to improve aromatic amino acids producing strains.
文摘Background: The physiological and operative severity score for the enumeration of mortality and morbidity (POSSUM) is a scoring system previously validated in vascular and general surgical patients. The Portsmouth physiological and operative severity score for the enumeration of mortality and morbidity (P-POSSUM) is a reliable tool derived from POSSUM to improve the prediction of mortality. In this paper, we utilized both models to determine the most suitable and feasible one that could be utilized in orthopedic surgery in China mainland. Methods: In this retrospective study, patients’ files were extracted randomly from the medical records department of the First Hospital Affiliated with Dalian Medical University for those underwent hip replacements between 1999 and 2006. The mortality and morbidity rates were predicted by P-POSSUM and POSSUM, respectively. A comparative analysis was performed between the observed and the predicted values as well as the Observed/Expected ratio (O:E). Results: A total of 206 patients were selected for this study after fulfilling the inclusion and exclusion criteria. The predicted mortality by P-POSSUM were not significantly different from the observed values (X2 = 2.10, P = 0.552). POSSUM appeared to be better with the observed morbidity (X2 = 2.766, P = 0.598), but had overestimated mortality. Conclusion: The POSSUM morbidity equation satisfactorily estimated the risk of postoperative complications, and the P-POSSUM mortality equation appeared to stratify the risk of death more accurate than the POSSUM.
文摘Reducing drag during take-off and nominal(cruise)conditions is a problem of fundamental importance in aeronautical engineering.Existing studies have demonstrated that v-shaped symmetrical riblets can effectively be used for turbulence control,with those with dimensionless depth h+=15 and dimensionless width s+=15 having the best drag reduction effect.In the present study,experimental tests have been conducted considering two models of the same size,one with smooth surface,the other with v-shaped riblets of the h+=15 and s+=15 type.The results show that for an angle of attack in the 8°~20°range(take-off stage),the maximum lift coefficient can be increased by 22%.For angle of attack between 8°and 14°,a drag reduction effect can be produced using riblets,which increases with the Reynolds number,leading to a decrease in the drag coefficient maximum of 36%.Flow visualization experiments have been carried out by means of Laser Induced fluorescence.
文摘This study investigates the predictability of a fixed uncertainty index(UI)for realized variances(volatility)in the international stock markets from a high-frequency perspective.We construct a composite UI based on the scaled principal component analysis(s-PCA)method and demonstrate that it exhibits significant in-and out-of-sample predictabilities for realized variances in global stock markets.This predictive power is more powerful than those of two commonly employed competing methods,namely,PCA and the partial least squares(PLS)methods.The result is robust in several checks.Further,we explain that s-PCA outperforms other dimension-reduction methods since it can effectively increase the impacts of strong predictors and decrease those of weak factors.The implications of this research are significant for investors who allocate assets globally.
文摘The material composition of geological samples is very complicated. Generally, the method for accurately determining trace elements in geological samples needs to digest the solid samples into liquid state, which is convenient for inductively coupled plasma mass spectrometry. The previous digestion method takes a long time and the operation process is complicated. The Ultra CLAVE microwave digestion instrument was used to digest the geological samples. The types and dosages of the acid used for digestion and the optimal conditions for instrumental testing were published. The results show that this method has short processing time, small data error, safer operation and good digestion effect. This method is suitable for geological sample analysis.
文摘Stone coal is a special resource, because it is associated with a variety of rare metal elements. The elemental geochemical characteristics of stone coal can provide useful information for the study of its formation and evolution history. The content of rare earth elements in stone coal has been the focus of attention. Stone coal is widely distributed in southern Shaanxi province of China. In order to study the content and distribution of rare earth elements in stone coal, and provide a plan for the comprehensive development and utilization of stone coal, this study tested the rare earth elements content of stone coal, parting and coal ash samples, respectively, compared the content of rare earth elements in stone coal, parting, coal ash with the mean value of China coal, the mean value of world coal and the abundance value of upper crust, and discussed the features of rare earth elements content in different types of samples in southern Shaanxi province of China. The average contents of rare earth elements in the samples of stone coal, parting and coal ash were calculated, and the enrichment coefficients of the samples with the mean value of China coal, the mean value of world coal and the abundance value of upper crust were listed. The distribution rules and characteristics of the contents of rare earth elements in different types of samples in southern Shaanxi province of China were summarized.
基金supported by the National Natural Science Foundation of China(Grant No.41871164)the National Key Research and Development Program of China(Grant No.2018YFC1508803)+2 种基金the Major Program of National Social Science Fund of China(Grant No.18ZDA105)the Shanghai Philosophy and Social Science Planning Program(Grant No.2021XRM005)the Fundamental Research Funds for the Central Universities(Grant No.2022ECNU-XWK-XK001)。
文摘Increasing urban pluvial flood disasters due to climate change and rapid urbanisation have been a great challenge worldwide.Timely and effective emergency evacuation is important for reducing casualties and losses.This has become a bottleneck for emergency management.This study aimed to develop a commonly used Agent-Based Mode(ABM)for pluvial flood emergency evacuation at the city scale,exploring the cascading impacts of pluvial flooding on human behaviour and emergency evacuation.The July 2021 pluvial flood event in Zhengzhou,Henan Province,claiming 380 lives and 40.9 billion yuan in direct losses,was selected as this case study.A raster-based hydraulic model(ECNU Flood-Urban)was used to predict flood inundation(extent and depth)during an event in Zhengzhou’s centre.Moreover,a comparative analysis of emergency evacuations was conducted before and after the pluvial flood event.The results showed that crowd behaviour plays an important role in an emergency evacuation,and extensive flooding leads to an 11-83%reduction in the number of evacuees.This study highlights the importance of risk education and contingency plans in emergency response.The ABM model developed in this study is proven to be effective and practical and will provide support for decision-making in urban flood emergency management.
基金supported by the National Key R&D Program of China(2022YFA0807300)the National Natural Science Foundation of China(82271775 and 81971466)+1 种基金the Natural Science Foundation Outstanding Youth Fund of Jiangsu Province(BK20220049)and the CAMS Innovation Fund for Medical Sciences(CIFMS 2021-I2M-1-061,2021-I2M-1-047 and 2022-I2M-2-004).BZ was in part supported by the Innovation Capability Support Program of Shaanxi 2021TD-38.JZ was in part supported by a Translational Research Grant of NCRCH(2020ZKZC04)and the National Natural Science Foundation of China(82071765)supported by the Natural Science Foundation of China(NSFC 31900645).We thank Prof.Yonghong Wan from McMaster University,Canada,for his critical reading of the manuscript and helpful discussions.
文摘SEL1L-mediated endoplasmic reticulum-associated degradation(ERAD)plays critical roles in controlling protein homeostasis by degrading misfolded or terminal unfolded proteins.However,it remains unclear how SEL1L regulates peripheral T-cell survival and homeostasis.Herein,we found that SEL1L deficiency led to a greatly reduced frequency and number of mature T cells,which was further validated by adoptive transfer experiments or bone marrow chimera experiments,accompanied by the induction of multiple forms of cell death.Furthermore,SEL1L deficiency selectively disrupted naïve CD8+T-cell homeostasis,as indicated by the severe loss of the naïve T-cell subset but an increase in the memory T-cell subset.We also found that SEL1L deficiency fueled mTORC1/c-MYC activation and induced a metabolic shift,which was largely attributable to enhanced expression of the IL-15 receptorαandβchains.Mechanistically,single-cell transcriptomic profiling and biochemical analyses further revealed that Sel1l−/−CD8+T cells harbored excessive ER stress,particularly aberrant activation of the PERK-ATF4-CHOP-Bim pathway,which was alleviated by supplementing IL-7 or IL-15.Importantly,PERK inhibition greatly resolved the survival defects of Sel1l−/−CD8+T cells.In addition,IRE1αdeficiency decreased mTORC1 signaling in Sel1l−/−naïve CD8+T cells by downregulating the IL-15 receptorαchain.Altogether,these observations suggest that the ERAD adaptor molecule SEL1L acts as an important checkpoint for preserving the survival and homeostasis of peripheral T cells by regulating the PERK signaling cascade and IL-15 receptor-mediated mTORC1 axis.
基金supported by the National Key Research and Development Program of China(No.2021YFE0205800)the National Natural Science Foundation of China(Nos.12174292,11904267,and 91950110)the Fundamental Research Funds for the Central Universities(Nos.2042022kf1013,2042022kf0024,and 2042021kf0018)。
文摘For a conventional cascaded metasurface,the combination channel and each single channel are mutually dependent because the phase modulation of a cascaded metasurface is the sum of each single one.Here we propose a cascaded metasurface that can independently encode information into multiple channels.Based on the orientation degeneracy of anisotropic metasurfaces,each single metasurface can produce a quick-response(QR)image in the near field,governed by the Malus law,while the combined channel can produce a holographic image in the far field,governed by geometric phase.The independent and physically separated trichannel design makes information encryption safer.
文摘This study focuses on the decreasing relevance of financial information associated with current financial reporting standards for intangible assets.We summarize and compare three approaches to improving financial reporting standards for internally generated intangibles—the recognition approach,the fair value approach and the disclosure approach,among which we focus on the recognition approach.We investigate the impact of current International Accounting Standard 38 on the R&D capitalization policies of the high-tech industry,particularly among medical device firms in China.We conclude that the current recognition criteria are so stringent that they disincentivize firms from capitalizing their R&D investments.A large variation exists in capitalization timing within the medical device industry.Accordingly,we propose the milestone approach to revising financial reporting standards for intangible assets.We suggest that determining the capitalization criteria for intangibles based on the R&D cycle and capitalization timing should be moved forward.
文摘CE-1 Lunar Microwave Sounder (CELMS) is the first passive microwave radiometer in the world to sound the surface of the Moon in the lunar orbit at altitude of 200 km. The scientific objective of CELMS is to obtain global brightness temperature (TB) of the Moon, to retrieve information on lunar regolith, and to evaluate the distribution of helium-3 on the Moon implanted by solar wind. Before launch of CELMS, a series of experiments were carried out in laboratories to test the performances of the systems, and to calibrate the responses between the input of TB and the output of voltage from the receivers. However, the thermal condition exposed to CELMS is more complicated in lunar orbit than on the Earth, which makes the temperatures of different parts of CELMS wave vary greatly, and the cosmic background is not very clean due to the pointing of cold space antenna to the direction of the satellite running, which brings uncertainties into data-processing of CELMS when the temperature of cold space is used as a calibrator. Furthermore, the lack of knowledge on the lunar ingredients and compositions, distributions of physical temperatures, and properties on lunar microwave radiation leads to difficulties in validating the measurements and retrievals of CELMS. By analyzing the results of ground experiments and the measurements of CELMS in-orbit, along with our knowledge of the properties of lunar surface, here we give algorithms on calibration and antenna pattern correction (APC) of CELMS. We also describe in detail the principle of microwave transfer among the elements of CELMS, and discuss the method on testing calibration parameters of the system. In addition, the theory and model on correction antenna pattern of CELMS are developed by comparing antenna temperatures by CELMS with those simulated by microwave radiative transfer models. The global distribution of TB is given and the features of TB are analyzed. Our results show rich information included in TB on the properties of lunar regolith, especially the thickness and dielectric constant, which are nearly directly reflected by the differences of TB at day and those at night.
基金This study was supported by the National Natural Science Foundation of China （Grant No. 41001331）, The Programme of Introducing Talents of Discipline to University （111 Project） and the Natural Science Foundation of Shanghai （Grant No. 15ZR1428700）. The authors thank Professor Lu Ming and Zhou Limin of East China Normal University for their help with geochemical analysis. Thanks to Cashion for the language modification.
文摘This study presents the results obtained from magnetic susceptibility and heavy metal （Cu, Zn, Pb, and Cr） concentration measurements of soil profiles collected from arable land and urban parks in Baoshan District, an industrial district of Shanghai, China. The study focuses on the investigation of vertical variations in magnetic susceptibilities and heavy metal concentrations and on correlations between magnetic susceptibilities and heavy metal concentrations in soil profiles. The results demon- strate that magnetic enhancement in the surface layer of the soil profile is associated with increased heavy metal pollution. The enrichment factors （EF） and the Tomlinson Pollution Load Index （PLI-EF） are calculated for estimat- ing the level of heavy metal pollution of soil profiles in the study. The significant positive correlations between heavy metal contents, enrichment factors （EF）, Tomlinson pollution load index （PLI-CF）, modified Tomlinson pollution load index （PLI-EF）, and magnetic susceptibility （X） indicate that much of the heavy metal contamination in the study area is linked to combustion derived particulate emissions. The results confirm that the combined magnetic measurement and heavy metal concentration analysis could provide useful information for soil monitoring in urban environments. However, the use of magnetic technique to locate the heavy metal pollution boundary in the soil profile of this studied area should be confirmed by further geochemical analysis.
基金supported by the National Science and Technology Specific Projects(NO.2018ZX10301407)the National Natural Science Foundation of China(NO.82002147 and NO.82073618)the Project founded by China Postdoctoral Science Foundation(NO.2019M662543).
文摘On 12 March 2020,the outbreak of coronavirus disease 2019(COVID-19)was declared a pandemic by the World Health Organization.As of 4 August 2020,more than 18 million confirmed infections had been reported globally.Most patients have mild symptoms,but some patients develop respiratory failure which is the leading cause of death among COVID-19 patients.Endothelial cells with high levels of angiotensin-converting enzyme 2 expression are major participants and regulators of inflammatory reactions and coagulation.Accumulating evidence suggests that endothelial activation and dysfunction participate in COVID-19 pathogenesis by altering the integrity of vessel barrier,promoting pro-coagulative state,inducing endothelial inflammation,and even mediating leukocyte infiltration.This review describes the proposed cellular and molecular mechanisms of endothelial activation and dysfunction during COVID-19 emphasizing the principal mediators and therapeutic implications.
基金This work was supported by Sichuan Provincial Youth Science Fund, the Science and Technology on Communication Security Laboratory Project （9140C110201110C1102）, the National Natural Science Foundations of China （Grant Nos. 61003299, 61202437）, the Natural Sci- ence Basic Research Plan in Shaanxi Province of China （2012JM8041）, the Fundamental Research Funds for the Central Universities （K5051201036） and the ＂l 1 l＂ Project （B08038）. Thanks are due to anonymous referees for a series of comment on this paper.
文摘The global avalanche characteristics （the sum- of-squares indicator and the absolute indicator） measure the overall avalanche characteristics of a cryptographic Boolean function. Sung et al. （1999） gave the lower bound on the sum- of-squares indicator for a balanced Boolean function satisfy- ing the propagation criterion with respect to some vectors. In this paper, if balanced Boolean functions satisfy the propaga- tion criterion with respect to some vectors, we give three nec- essary and sufficient conditions on the auto-correlation distri- bution of these functions reaching the minimum the bound on the sum-of-squares indicator. And we also find all Boolean functions with 3-variable, 4-variable, and 5-variable reaching the minimum the bound on the sum-of-squares indicator.
基金supported by National Natural Science Foundation of China (Grant No. 40601066)Chinese "Chang’E-1" Project Microwave Radiometer Item
文摘Investigation on Lunar polar area is almost every lunar mission’s primary objective in recent years. The rationale behind it is that illumination and ice resources in this area can be potentially very helpful for constructing lunar human base. In this paper, we analyze microwave radiometric characteristics of the Moon by using the newly acquired Chang’E-1 Lunar Microwave Sounder (CELMS) data. Microwave brightness temperature at Lunar South Pole (LSP) is distributed regularly with a style of "ring-in-ring", decreasing from equator to pole. Regolith temperature gradient is bigger at lunar equator than at polar area. Brightness temperature diurnal difference decreases with observation frequency. Microwave brightness temperature distribution maps at LSP and Lunar North Pole (LNP) have been made based on the analysis. It is found that microwave brightness temperature becomes to synchronize with elevation beyond -85° latitude. This phenomenon is related to lightening condition and indicates temperature distribution at LSP. The brightness temperature anomaly cold points are potentially cold trap areas for water or ice while hot points imply plenty of illumination resources there.
基金This study was sponsored by the National Key R&D Program of China(2017YFA0604802,2019YFE0117000)the National Natural Science Foundation of China(41320104002).
文摘Aims Both extreme drought and insect herbivores can suppress plant growth in grassland communities.However,most studies have examined extreme drought and insects in isolation,and there is reason to believe that insects might alter the ability of grasslands to withstand drought.Unfortunately,few studies have tested the interactive effects of extreme drought and insect herbivores in grassland communities.Methods Here,we tested the drought–herbivore interactions using a manipulative experiment that factorially crossed extreme drought with the exclusion of insect herbivores in a temperate semiarid grassland in Inner Mongolia.Important Findings Our results demonstrated that both extreme drought and insect herbivores separately decreased total plant cover.When combined,insect herbivores reduced the impact of drought on total cover by increasing the relative abundance of drought-resistant dominant species.Our results highlight that the negative effect of extreme drought on total plant cover could be alleviated by maintaining robust insect herbivore communities.
