In order to evaluate the electromagnetic environment of 5G base station, measurement and evaluation of the electromagnetic environment are studied. The 12 measuring points are chosen on the roof, inside and outside of...In order to evaluate the electromagnetic environment of 5G base station, measurement and evaluation of the electromagnetic environment are studied. The 12 measuring points are chosen on the roof, inside and outside of the building, which has a 5G base station on the top. The electric field intensity, magnetic field intensity, and power density have been measured. The measurement methods include background measurement and work measurement. Background measurement is the measurement of environmental electromagnetic field (EMF) before the installation of 5G base station while the working measurement is the measurement after the installation of 5G base station. The evaluation methods include t-test for qualitative evaluation and electromagnetic gain for quantitative evaluation. The results show that the electromagnetic environment after the installation of 5G base station in most places is different from that in the background. And the environmental electromagnetic fields in certain parts are lower than those in the background. The conclusions are as follows: 1) The electromagnetic environment of 5G base station is far lower than the control limit of the national standard and conforms to the national standard;2) The electromagnetic environment of 5G base station has little impact on the electromagnetic environment;3) It is not sufficient to assume that 5G is harmful to health without the results of the epidemiological investigation;4) Before the construction of 5G base station, do background EMF detection, which can provide support for future evaluation.展开更多
N6-甲基腺苷(m6A)是RNA中最普遍的表观遗传修饰。甲基化酶样3(methyltransferase like 3,METTL3)作为最主要的甲基化酶,参与m6A所调节的RNA的剪接、运输、翻译和降解等生物过程。目前,诸多研究证实METTL3在人类癌症中发挥着重要作用且...N6-甲基腺苷(m6A)是RNA中最普遍的表观遗传修饰。甲基化酶样3(methyltransferase like 3,METTL3)作为最主要的甲基化酶,参与m6A所调节的RNA的剪接、运输、翻译和降解等生物过程。目前,诸多研究证实METTL3在人类癌症中发挥着重要作用且影响癌症进展。METTL3能够通过多种机制调控癌症的发生发展:影响癌细胞甲基化水平、mRNA稳定性、调节癌细胞转化、相关信号通路、细胞凋亡等。因此,深入研究癌症发展机制,阐明METTL3调节癌症的发病机理,对癌症诊疗具有重要的理论意义和应用价值。本文通过对METTL3在多种癌症中的调节机制进行整理和分析,并总结了METTL3在各种癌症发生和发展中的最新研究进展。展开更多
The development of novel organic electrode materials is of great significance for improving the reversible capacity and cycle stability of rechargeable batteries.Before practical application,it is essential to charact...The development of novel organic electrode materials is of great significance for improving the reversible capacity and cycle stability of rechargeable batteries.Before practical application,it is essential to characterize the electrode materials to study their structures,redox mechanisms and electrochemical performances.In this review,the common characterization methods that have been adopted so far are summarized from two aspects:experimental characterization and theoretical calculation.The experimental characterization is introduced in detail from structural characterization,electrochemical characterization and electrode reaction chara cterization.The experimental purposes and working principles of various experimental characterization methods are briefly illustrated.As the auxilia ry means,theoretical calculation provides the theoretical basis for characterizing the electrochemical reaction mechanism of organic electrode materials.Through these characterizations,we will have a deep understanding about the material structures,electrochemical redox mechanisms,electrochemical properties and the relationships of structure-property.It is hoped that this review would help researchers to select the suitable characterization methods to analyze the structures and performances of organic electrode materials quickly and effectively.展开更多
Most of previous video recording devices in mobile vehicles commonly store captured video contents locally. With the rapid development of 4G/Wi Fi networks, there emerges a new trend to equip video recording devices w...Most of previous video recording devices in mobile vehicles commonly store captured video contents locally. With the rapid development of 4G/Wi Fi networks, there emerges a new trend to equip video recording devices with wireless interfaces to enable video uploading to the cloud for video playback in a later time point. In this paper, we propose a QoE-aware mobile cloud video recording scheme in the roadside vehicular networks, which can adaptively select the proper wireless interface and video bitrate for video uploading to the cloud. To maximize the total utility, we need to design a control strategy to carefully balance the transmission cost and the achieved QoE for users. To this purpose, we investigate the tradeoff between cost incurred by uploading through cellular networks and the achieved QoE of users. We apply the optimization framework to solve the formulated problem and design an online scheduling algorithm. We also conduct extensive trace-driven simulations and our results show that our algorithm achieves a good balance between the transmission cost and user QoE.展开更多
Organic small structure quinones go with ionic liquids electrolytes would exhibit ultrastable electrochemical properties.In this study,calix[6]quinone(C6Q) cathode was matched with ionic liquid electrolyte Li[TFSI]/[P...Organic small structure quinones go with ionic liquids electrolytes would exhibit ultrastable electrochemical properties.In this study,calix[6]quinone(C6Q) cathode was matched with ionic liquid electrolyte Li[TFSI]/[PY13][TFSI](bis(trifluoromethane)sulfonimide lithium salt/N-methyl-N-pro pylpyrrolidinium bis(trifluoromethanesulfonyl)amide) to assemble lithium-ion batteries(LIBs).The electrochemical performance of LIBs was systematically studied.The capacity retention rates of C6Q through 1000 cycles at current densities of 0.2 C and 0.5 C were 70% and 72%,respectively.At 5 C, the capacity was maintained at 190 mAh g^(-1) after 1000 cycles,and 155 mAh g^(-1) even after 10,000 cycles,comparable to inorganic materials.This work would give a big push to the practical process of organic electrode materials in energy storage.展开更多
Objective: Mifepristone, as a type of well-accepted emergency contraceptive drugs, is an optional progesterone receptor mod-ulator and widely used clinically. However, the effect of mifepristone on sperms is poorly st...Objective: Mifepristone, as a type of well-accepted emergency contraceptive drugs, is an optional progesterone receptor mod-ulator and widely used clinically. However, the effect of mifepristone on sperms is poorly studied. This research is intended to investigate the biological effect of mifepristone on human sperm function in vitro. Methods: Spermatozoa were incubated in the culture medium containing 0.04 μM, 0.4 μM, 4 μM and 40 μM of mifepristone for one hour after capacitation. It was required to measure sperm motility, viability, DNA integrity, acrosomal reaction, sperm hyperactivation, and the concentration of free calcium ions in spermatozoa. Results: After treating sperms with different concentrations of mifepristone in vitro, sperm viability, motility, morphology and DNA integrity were measured. The differences between the control group and the experimental group were of no statistical significance (p > .05). When the concentration of mifepristone was higher than 0.4 μM, the proportion of sperm hyperactivation was significantly reduced (p < .01). According to further examination on the rate of acrosomal reaction (AR rate) and the con-centration of free calcium ions in sperms, it was found that acrosomal reaction was significantly inhibited with the concentration of free calcium ions in sperms decreased when the concentration of mifepristone was higher than 0.4 μM. Conclusions: The in-vitro spermicidal mechanism of mifepristone is realized by inhibiting acrosomal reaction, hyperactivation and decreasing the concentration of free calcium ions.展开更多
Objective:To explore the potential effect of melatonin on the in-vitro maturation of mouse oocytes under heat shock condition.Methods:This study used a heat shock model of mouse oocyte maturation.The oocytes were rand...Objective:To explore the potential effect of melatonin on the in-vitro maturation of mouse oocytes under heat shock condition.Methods:This study used a heat shock model of mouse oocyte maturation.The oocytes were randomly divided into three groups:control group,heat shock group and heat shock+melatonin group,in order to evaluate the effect of 1×10^(−9) mol/L melatonin on the quality of oocytes after heat shock.Results:In comparison with the control group,the maturation rate of mouse oocytes in heat shock group was significantly decreased[(33.00±0.07)%vs.(85.00±0.03)%,p<0.01],with abnormal spindle assembly,and the early apoptosis rate was significantly increased[(59.7±4.5)%vs.(22.0±3.5)%,p<0.01].Compared with heat shock group,the maturation rate of oocytes was significantly increased in heat shock+melatonin group[(70.00±0.05)%vs.(33.00±0.07)%,p<0.01],meanwhile,the spindle abnormality rate and the early apoptosis rate were significantly decreased accordingly[(37.3±6.1)%vs.(59.7±4.5)%,p<0.01].The expression level of heat shock protein 70 was significantly up-regulated in heat shock+melatonin group in comparison with other two groups(p<0.01).Conclusions:By regulating the over-expression of heat shock protein 70,melatonin can improve the declined maturation rate of oocytes and the increased rates of spindle assembly abnormality and early apoptosis caused by heat shock.展开更多
Objective:To explore immunotherapy effectiveness of the CD4^(+)CD25^(+) regulatory T cells for treating female mouse with recurrent spontaneous abortion(RSA)by animal experiments.Methods:Mononuclear lymphocytes were i...Objective:To explore immunotherapy effectiveness of the CD4^(+)CD25^(+) regulatory T cells for treating female mouse with recurrent spontaneous abortion(RSA)by animal experiments.Methods:Mononuclear lymphocytes were isolated from the blood(instead of cord blood)of new-born baby of KunMing Bai mouse or BALB/c male mouse with normal birth ability(as unrelated third party blood source)by density gradient centrifuga-tion method.The CD4^(+)CD25^(+) regulatory T cells were selected by magnetic-activated cell sorting from mononuclear cells of cord blood cells.CBA/J female mouse copulated with DBA/2J male mouse was utilized as RSA animal model.Pregnant RSA mice were injected different types of lymphocytes through tail vein.Independent sample t-test was used to analyze the data from each group.Results:The proportion of CD4^(+)CD25^(+)T cells in CD4^(+)T cells was(17.49±0.60)%in CD4^(+)CD25^(+) regulatory T cells injection group,which was statistical significant higher than that of mononuclear lymphocyte injection group(14.68±0.83)%,sterile PBS group(9.54±0.85)%or no injection group(9.28±0.68)%(p<.05,t-value was 4.754,13.242 and 15.621,respec-tively).The Foxp3 relative protein expression level of CD4^(+)CD25^(+) regulatory T cells injected group was 5.85±0.45,which was also significant higher than that of mononuclear lymphocyte injection(2.86±0.54),sterile PBS group(1.08±0.16)or no injection group(1.00±0.00)(p<.05,t-value was 7.276,17.227 and 18.635,respectively).Finally,two times of CD4^(+)CD25^(+)T cell injected group at the 4 th and 8 th day had well effect for RSA mouse,and embryo sorption rate was(4.92±0.08)%,which significant lower than that of two times of mononuclear lymphocyte injected group(13.07±0.06)%,sterile PBS group(23.11±0.12)%,or no injection group(25.47±0.11)%(p<.05,t-value was-2.603,-4.012 and-4.700,respectively).Conclusions:Pregnant mouse with RSA injected CD4^(+)CD25^(+)T cells several times for immunotherapy can get better effec-tiveness than that of pregnant mouse injected traditional mononuclear cells.展开更多
Covalent organic frameworks(COFs)have garnered increasing attention as promising electrode materials for sodium-ion batteries(SIBs)due to their ordered backbones,uniform pore sizes,and high surface areas.However,they ...Covalent organic frameworks(COFs)have garnered increasing attention as promising electrode materials for sodium-ion batteries(SIBs)due to their ordered backbones,uniform pore sizes,and high surface areas.However,they also face challenges,including low capacity,unsatisfactory rate performance,and limited cycling stability,which pose the primary obstacles to their practical use.Herein,a pyrenoimidazole-based COF,denoted as PyIm-COF,has been synthesized as a novel electrode material for highperformance SIBs using an efficient synthetic strategy.With extended fully conjugated backbones and readily accessible redox-active sites,PyIm-COF demonstrates remarkable high-rate performance,delivering around 250 m Ah g^(−1)and excellent cycling stability,retaining 97.2%of its capacity even after 2500 cycles at 5 A g^(−1),which is higher than that of most previously reported COF-based SIBs.This work provides valuable insights into the development of nitrogen-rich conjugated COF electrode materials for rechargeable SIBs.展开更多
Organic lithium-ion batteries（OLIBs） represent a new generation of power storage approach for their environmental benignity and high theoretical specific capacities.However, it has the disadvantage with regard to th...Organic lithium-ion batteries（OLIBs） represent a new generation of power storage approach for their environmental benignity and high theoretical specific capacities.However, it has the disadvantage with regard to the dissolution of active materials in organic electrolyte. In this study, we encapsulated high capacity material calix[4]quinone（C4Q） in the nanochannels of ordered mesoporous carbon（OMC）CMK-3 with various mass ratios ranging from 1：3 to 3：1, and then systematically investigated their morphology and electrochemical properties. The nanocomposites characterizations confirmed that C4Q is almost entirely capsulated in the nanosized pores of the CMK-3 while the mass ratio is less than2：1. As cathodes in lithium-ion batteries, the C4Q/CMK-3（1：2） nanocomposite exhibits optimal initial discharge capacity of 427 mA h g^（-1） with 58.7% cycling retention after 100 cycles. Meanwhile, the rate performance is also optimized with a capacity of 170.4 mA h g^（-1） at 1 C. This method paves a new way to apply organic cathodes for lithium-ion batteries.展开更多
Organic quinone compounds have attracted wide attention due to their high theoretical capacities.Here,a novel cyclic macromolecular calix[6]quinone(C6Q),which possesses 6 p-quinone units and can provide 12 electrochem...Organic quinone compounds have attracted wide attention due to their high theoretical capacities.Here,a novel cyclic macromolecular calix[6]quinone(C6Q),which possesses 6 p-quinone units and can provide 12 electrochemical active sites,has been applied as a promising cathode material in lithium ion batteries(LIBs).The as-fabricated LIBs exhibited an initial specific capacity as high as 423 mA h g^-1(Ctheo=447 mA h g^-1)at 0.1 C.After 100 cycles,the capacity of C6Q maintained at 216 mA h g^-1,and even after 300 cycles,C6Q still achieved a high specific capacity of 195 mA h g^-1 with negligible capacity fading(as compared with the 100th cycle).Due to the large capacity and wide electrochemical window,C6Q can deliver a specific energy up to 1201 W h kg^-1.In addition,the method of immobilizing C6Q with ordered mesoporous carbon(OMC)CMK-3 could further enhance the electrochemical performance of C6Q.展开更多
Covalent organic frameworks(COFs)with periodic channels and tunable chemical structures have been widely considered as promising electrode materials in rechargeable batteries.However,the design and construction of hig...Covalent organic frameworks(COFs)with periodic channels and tunable chemical structures have been widely considered as promising electrode materials in rechargeable batteries.However,the design and construction of high-performance COFs-based electrodes still face some challenges in the introduction of multiple efficient redox centers as well as the reduction of dead mass.To address these issues,a unique COF containing double active centers(C═N and N═N)is developed as an anode in rechargeable lithium-ion batteries(LIBs).The as-prepared COF displays excellent electrochemical performance due to its remarkable structural stability and the existence of many active groups.Meanwhile,its electrochemical performance is significantly better than that of the small molecule compound or the linear polymer with the same construction units.Even at a high current density of 5 A/g,the LIBs with COF electrodes remain at a high discharge capacity of 227 mAh/g after 2000 cycles.Moreover,the distinction in electrochemical performances of these three materials is further revealed by calculation.This study illustrates the importance of molecular structure design for improving the performance of organic electrodes.展开更多
Late-stage melanoma is refractory to current therapies. MicroRNAs (miRNAs) can modulate many physiological and pathological processes of melanoma. Studies have demonstrated that miR-137 acts as a tumor suppressor by i...Late-stage melanoma is refractory to current therapies. MicroRNAs (miRNAs) can modulate many physiological and pathological processes of melanoma. Studies have demonstrated that miR-137 acts as a tumor suppressor by inhibiting the proliferation of melanoma cells through targeting multiple mRNAs. The glyoxalase system member glyoxalase 1 (GLO1) is the principal scavenging enzyme of methylglyoxal (MG), a toxic byproduct of glycolysis. Using 35S in vivo/vitro labelling analysis for dynamic proteomics (SiLAD), we found that miR-137 downregulated the expression of GLO1 in melanoma cells.Bioinformatics analysis predicted that GLO1 is a direct target of miR-137. This was validated by dual luciferase reporter assay.Quantitative RT-PCR (qRT-PCR) and western blot analysis indicated that miR-137 could decrease endogenous GLO1 expression.Furthermore, siRNA targeting of GLO1 mimicked inhibition of melanoma cell proliferation caused by miR-137 overexpression.Re-expression of GLO1 was able to restore miR-137-mediated suppression of melanoma cell proliferation. Therefore, these results suggest that miR-137 inhibits the proliferation of melanoma cells by targeting GLO1.展开更多
文摘In order to evaluate the electromagnetic environment of 5G base station, measurement and evaluation of the electromagnetic environment are studied. The 12 measuring points are chosen on the roof, inside and outside of the building, which has a 5G base station on the top. The electric field intensity, magnetic field intensity, and power density have been measured. The measurement methods include background measurement and work measurement. Background measurement is the measurement of environmental electromagnetic field (EMF) before the installation of 5G base station while the working measurement is the measurement after the installation of 5G base station. The evaluation methods include t-test for qualitative evaluation and electromagnetic gain for quantitative evaluation. The results show that the electromagnetic environment after the installation of 5G base station in most places is different from that in the background. And the environmental electromagnetic fields in certain parts are lower than those in the background. The conclusions are as follows: 1) The electromagnetic environment of 5G base station is far lower than the control limit of the national standard and conforms to the national standard;2) The electromagnetic environment of 5G base station has little impact on the electromagnetic environment;3) It is not sufficient to assume that 5G is harmful to health without the results of the epidemiological investigation;4) Before the construction of 5G base station, do background EMF detection, which can provide support for future evaluation.
文摘N6-甲基腺苷(m6A)是RNA中最普遍的表观遗传修饰。甲基化酶样3(methyltransferase like 3,METTL3)作为最主要的甲基化酶,参与m6A所调节的RNA的剪接、运输、翻译和降解等生物过程。目前,诸多研究证实METTL3在人类癌症中发挥着重要作用且影响癌症进展。METTL3能够通过多种机制调控癌症的发生发展:影响癌细胞甲基化水平、mRNA稳定性、调节癌细胞转化、相关信号通路、细胞凋亡等。因此,深入研究癌症发展机制,阐明METTL3调节癌症的发病机理,对癌症诊疗具有重要的理论意义和应用价值。本文通过对METTL3在多种癌症中的调节机制进行整理和分析,并总结了METTL3在各种癌症发生和发展中的最新研究进展。
基金the financial support of the National Natural Science Foundation of China(Nos.21875206,21403187)the Natural Science Foundation of Hebei Province(No.B2019203487)the open project in Key Lab Adv.Energy Mat.Chem.(Nankai University)。
文摘The development of novel organic electrode materials is of great significance for improving the reversible capacity and cycle stability of rechargeable batteries.Before practical application,it is essential to characterize the electrode materials to study their structures,redox mechanisms and electrochemical performances.In this review,the common characterization methods that have been adopted so far are summarized from two aspects:experimental characterization and theoretical calculation.The experimental characterization is introduced in detail from structural characterization,electrochemical characterization and electrode reaction chara cterization.The experimental purposes and working principles of various experimental characterization methods are briefly illustrated.As the auxilia ry means,theoretical calculation provides the theoretical basis for characterizing the electrochemical reaction mechanism of organic electrode materials.Through these characterizations,we will have a deep understanding about the material structures,electrochemical redox mechanisms,electrochemical properties and the relationships of structure-property.It is hoped that this review would help researchers to select the suitable characterization methods to analyze the structures and performances of organic electrode materials quickly and effectively.
基金supported in part by the National Science Foundation of China under Grant 61272397,Grant 61572538,Grant 61174152,Grant 61331008in part by the Guangdong Natural Science Funds for Distinguished Young Scholar under Grant S20120011187
文摘Most of previous video recording devices in mobile vehicles commonly store captured video contents locally. With the rapid development of 4G/Wi Fi networks, there emerges a new trend to equip video recording devices with wireless interfaces to enable video uploading to the cloud for video playback in a later time point. In this paper, we propose a QoE-aware mobile cloud video recording scheme in the roadside vehicular networks, which can adaptively select the proper wireless interface and video bitrate for video uploading to the cloud. To maximize the total utility, we need to design a control strategy to carefully balance the transmission cost and the achieved QoE for users. To this purpose, we investigate the tradeoff between cost incurred by uploading through cellular networks and the achieved QoE of users. We apply the optimization framework to solve the formulated problem and design an online scheduling algorithm. We also conduct extensive trace-driven simulations and our results show that our algorithm achieves a good balance between the transmission cost and user QoE.
文摘目的:比较老年轻度认知障碍(mild cognitive impairment,MCI)者与老年认知正常对照(normal control,NC)者的听配能差异,探索其与抑郁、焦虑程度的相关性。方法:纳入老年MCI患者35例,NC组46例,采用言语、空间与听觉质量量表(speech,spatial and qualities of hearing scale,SSQ)评估其听配能各维度,同时对其认知功能与焦虑抑郁情况进行测评。结果:MCI组SSQ量表的言语理解与空间听觉维度分别显著低于NC组。两组言语理解能力均与抑郁程度显著负相关,MCI组的空间听觉能力与焦虑程度显著负相关(均为P<0.05)。结论:老年MCI患者的总体听配能弱于认知正常者,且听配能情况与抑郁焦虑程度相关,提升听配能可能有助于改善老年MCI患者认知功能与情绪状态。
基金supported by the National Natural Science Foundation of China (Nos. 21875206 and 21403187)the Natural Science Foundation of Hebei Province (B2019203487)。
文摘Organic small structure quinones go with ionic liquids electrolytes would exhibit ultrastable electrochemical properties.In this study,calix[6]quinone(C6Q) cathode was matched with ionic liquid electrolyte Li[TFSI]/[PY13][TFSI](bis(trifluoromethane)sulfonimide lithium salt/N-methyl-N-pro pylpyrrolidinium bis(trifluoromethanesulfonyl)amide) to assemble lithium-ion batteries(LIBs).The electrochemical performance of LIBs was systematically studied.The capacity retention rates of C6Q through 1000 cycles at current densities of 0.2 C and 0.5 C were 70% and 72%,respectively.At 5 C, the capacity was maintained at 190 mAh g^(-1) after 1000 cycles,and 155 mAh g^(-1) even after 10,000 cycles,comparable to inorganic materials.This work would give a big push to the practical process of organic electrode materials in energy storage.
文摘Objective: Mifepristone, as a type of well-accepted emergency contraceptive drugs, is an optional progesterone receptor mod-ulator and widely used clinically. However, the effect of mifepristone on sperms is poorly studied. This research is intended to investigate the biological effect of mifepristone on human sperm function in vitro. Methods: Spermatozoa were incubated in the culture medium containing 0.04 μM, 0.4 μM, 4 μM and 40 μM of mifepristone for one hour after capacitation. It was required to measure sperm motility, viability, DNA integrity, acrosomal reaction, sperm hyperactivation, and the concentration of free calcium ions in spermatozoa. Results: After treating sperms with different concentrations of mifepristone in vitro, sperm viability, motility, morphology and DNA integrity were measured. The differences between the control group and the experimental group were of no statistical significance (p > .05). When the concentration of mifepristone was higher than 0.4 μM, the proportion of sperm hyperactivation was significantly reduced (p < .01). According to further examination on the rate of acrosomal reaction (AR rate) and the con-centration of free calcium ions in sperms, it was found that acrosomal reaction was significantly inhibited with the concentration of free calcium ions in sperms decreased when the concentration of mifepristone was higher than 0.4 μM. Conclusions: The in-vitro spermicidal mechanism of mifepristone is realized by inhibiting acrosomal reaction, hyperactivation and decreasing the concentration of free calcium ions.
文摘Objective:To explore the potential effect of melatonin on the in-vitro maturation of mouse oocytes under heat shock condition.Methods:This study used a heat shock model of mouse oocyte maturation.The oocytes were randomly divided into three groups:control group,heat shock group and heat shock+melatonin group,in order to evaluate the effect of 1×10^(−9) mol/L melatonin on the quality of oocytes after heat shock.Results:In comparison with the control group,the maturation rate of mouse oocytes in heat shock group was significantly decreased[(33.00±0.07)%vs.(85.00±0.03)%,p<0.01],with abnormal spindle assembly,and the early apoptosis rate was significantly increased[(59.7±4.5)%vs.(22.0±3.5)%,p<0.01].Compared with heat shock group,the maturation rate of oocytes was significantly increased in heat shock+melatonin group[(70.00±0.05)%vs.(33.00±0.07)%,p<0.01],meanwhile,the spindle abnormality rate and the early apoptosis rate were significantly decreased accordingly[(37.3±6.1)%vs.(59.7±4.5)%,p<0.01].The expression level of heat shock protein 70 was significantly up-regulated in heat shock+melatonin group in comparison with other two groups(p<0.01).Conclusions:By regulating the over-expression of heat shock protein 70,melatonin can improve the declined maturation rate of oocytes and the increased rates of spindle assembly abnormality and early apoptosis caused by heat shock.
文摘Objective:To explore immunotherapy effectiveness of the CD4^(+)CD25^(+) regulatory T cells for treating female mouse with recurrent spontaneous abortion(RSA)by animal experiments.Methods:Mononuclear lymphocytes were isolated from the blood(instead of cord blood)of new-born baby of KunMing Bai mouse or BALB/c male mouse with normal birth ability(as unrelated third party blood source)by density gradient centrifuga-tion method.The CD4^(+)CD25^(+) regulatory T cells were selected by magnetic-activated cell sorting from mononuclear cells of cord blood cells.CBA/J female mouse copulated with DBA/2J male mouse was utilized as RSA animal model.Pregnant RSA mice were injected different types of lymphocytes through tail vein.Independent sample t-test was used to analyze the data from each group.Results:The proportion of CD4^(+)CD25^(+)T cells in CD4^(+)T cells was(17.49±0.60)%in CD4^(+)CD25^(+) regulatory T cells injection group,which was statistical significant higher than that of mononuclear lymphocyte injection group(14.68±0.83)%,sterile PBS group(9.54±0.85)%or no injection group(9.28±0.68)%(p<.05,t-value was 4.754,13.242 and 15.621,respec-tively).The Foxp3 relative protein expression level of CD4^(+)CD25^(+) regulatory T cells injected group was 5.85±0.45,which was also significant higher than that of mononuclear lymphocyte injection(2.86±0.54),sterile PBS group(1.08±0.16)or no injection group(1.00±0.00)(p<.05,t-value was 7.276,17.227 and 18.635,respectively).Finally,two times of CD4^(+)CD25^(+)T cell injected group at the 4 th and 8 th day had well effect for RSA mouse,and embryo sorption rate was(4.92±0.08)%,which significant lower than that of two times of mononuclear lymphocyte injected group(13.07±0.06)%,sterile PBS group(23.11±0.12)%,or no injection group(25.47±0.11)%(p<.05,t-value was-2.603,-4.012 and-4.700,respectively).Conclusions:Pregnant mouse with RSA injected CD4^(+)CD25^(+)T cells several times for immunotherapy can get better effec-tiveness than that of pregnant mouse injected traditional mononuclear cells.
基金the Natural Science Foundation of Liaoning province(grant no.2019-MS-046)W.Zhang thanks the University of Colorado Boulder for financial support of this work+1 种基金W.Huang acknowledges the support from the National Natural Science Foundation of China(grant no.21875206)The authors acknowledge the assistance of the Dalian University of Technology Instrumental Analysis Center.
文摘Covalent organic frameworks(COFs)have garnered increasing attention as promising electrode materials for sodium-ion batteries(SIBs)due to their ordered backbones,uniform pore sizes,and high surface areas.However,they also face challenges,including low capacity,unsatisfactory rate performance,and limited cycling stability,which pose the primary obstacles to their practical use.Herein,a pyrenoimidazole-based COF,denoted as PyIm-COF,has been synthesized as a novel electrode material for highperformance SIBs using an efficient synthetic strategy.With extended fully conjugated backbones and readily accessible redox-active sites,PyIm-COF demonstrates remarkable high-rate performance,delivering around 250 m Ah g^(−1)and excellent cycling stability,retaining 97.2%of its capacity even after 2500 cycles at 5 A g^(−1),which is higher than that of most previously reported COF-based SIBs.This work provides valuable insights into the development of nitrogen-rich conjugated COF electrode materials for rechargeable SIBs.
基金supported by the National Natural Science Foundation of China (21403187)the Natural Science Foundation of Hebei Province of China (B2015203124)the Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education), Nankai University
文摘Organic lithium-ion batteries（OLIBs） represent a new generation of power storage approach for their environmental benignity and high theoretical specific capacities.However, it has the disadvantage with regard to the dissolution of active materials in organic electrolyte. In this study, we encapsulated high capacity material calix[4]quinone（C4Q） in the nanochannels of ordered mesoporous carbon（OMC）CMK-3 with various mass ratios ranging from 1：3 to 3：1, and then systematically investigated their morphology and electrochemical properties. The nanocomposites characterizations confirmed that C4Q is almost entirely capsulated in the nanosized pores of the CMK-3 while the mass ratio is less than2：1. As cathodes in lithium-ion batteries, the C4Q/CMK-3（1：2） nanocomposite exhibits optimal initial discharge capacity of 427 mA h g^（-1） with 58.7% cycling retention after 100 cycles. Meanwhile, the rate performance is also optimized with a capacity of 170.4 mA h g^（-1） at 1 C. This method paves a new way to apply organic cathodes for lithium-ion batteries.
基金the financial support of the National Natural Science Foundation of China (21875206 and 21403187)China Postdoctoral Science Foundation (2015T80229)the Natural Science Foundation of Hebei Province (B2019203487)
文摘Organic quinone compounds have attracted wide attention due to their high theoretical capacities.Here,a novel cyclic macromolecular calix[6]quinone(C6Q),which possesses 6 p-quinone units and can provide 12 electrochemical active sites,has been applied as a promising cathode material in lithium ion batteries(LIBs).The as-fabricated LIBs exhibited an initial specific capacity as high as 423 mA h g^-1(Ctheo=447 mA h g^-1)at 0.1 C.After 100 cycles,the capacity of C6Q maintained at 216 mA h g^-1,and even after 300 cycles,C6Q still achieved a high specific capacity of 195 mA h g^-1 with negligible capacity fading(as compared with the 100th cycle).Due to the large capacity and wide electrochemical window,C6Q can deliver a specific energy up to 1201 W h kg^-1.In addition,the method of immobilizing C6Q with ordered mesoporous carbon(OMC)CMK-3 could further enhance the electrochemical performance of C6Q.
基金National Natural Science Foundation of China,Grant/Award Numbers:21875206,21403187。
文摘Covalent organic frameworks(COFs)with periodic channels and tunable chemical structures have been widely considered as promising electrode materials in rechargeable batteries.However,the design and construction of high-performance COFs-based electrodes still face some challenges in the introduction of multiple efficient redox centers as well as the reduction of dead mass.To address these issues,a unique COF containing double active centers(C═N and N═N)is developed as an anode in rechargeable lithium-ion batteries(LIBs).The as-prepared COF displays excellent electrochemical performance due to its remarkable structural stability and the existence of many active groups.Meanwhile,its electrochemical performance is significantly better than that of the small molecule compound or the linear polymer with the same construction units.Even at a high current density of 5 A/g,the LIBs with COF electrodes remain at a high discharge capacity of 227 mAh/g after 2000 cycles.Moreover,the distinction in electrochemical performances of these three materials is further revealed by calculation.This study illustrates the importance of molecular structure design for improving the performance of organic electrodes.
基金supported by the Special Funds for Major State Basic Research of China (2011CB915504)Coalition for National Science Funding (31171371 (2011–2015) to Dacheng He)he German Cancer Aid (Melanoma Research Network) to Dr. Stefan B. Eichmüller
文摘Late-stage melanoma is refractory to current therapies. MicroRNAs (miRNAs) can modulate many physiological and pathological processes of melanoma. Studies have demonstrated that miR-137 acts as a tumor suppressor by inhibiting the proliferation of melanoma cells through targeting multiple mRNAs. The glyoxalase system member glyoxalase 1 (GLO1) is the principal scavenging enzyme of methylglyoxal (MG), a toxic byproduct of glycolysis. Using 35S in vivo/vitro labelling analysis for dynamic proteomics (SiLAD), we found that miR-137 downregulated the expression of GLO1 in melanoma cells.Bioinformatics analysis predicted that GLO1 is a direct target of miR-137. This was validated by dual luciferase reporter assay.Quantitative RT-PCR (qRT-PCR) and western blot analysis indicated that miR-137 could decrease endogenous GLO1 expression.Furthermore, siRNA targeting of GLO1 mimicked inhibition of melanoma cell proliferation caused by miR-137 overexpression.Re-expression of GLO1 was able to restore miR-137-mediated suppression of melanoma cell proliferation. Therefore, these results suggest that miR-137 inhibits the proliferation of melanoma cells by targeting GLO1.
