Dear Editor,Generation of heterozygous genomes by hybridization between or within species can help maintain plant diversity and serve as a potential source of new species(Baek et al.,2018).Moreover,genomic heterozygos...Dear Editor,Generation of heterozygous genomes by hybridization between or within species can help maintain plant diversity and serve as a potential source of new species(Baek et al.,2018).Moreover,genomic heterozygosity is associated with genomic coadaptation,developmental stability,and heterosis.Accurate definition of alleles in haplotypes is necessary to precisely characterize allelic variation controlling agriculturally important traits(Shi et al.,2019).Currently,most released genomes have mosaic assembly of haplotypes due to random selection or collapse of alleles during genome assembly(Shi et al.,2019),which masked allelic variation and functional differentiation of divergent alleles in heterozygous species.展开更多
Plants absorb sunlight to power the photochem- ical reactions of photosynthesis, which can potentially damage the photosynthetic machinery. However, the mech- anism that protects chloroplasts from the damage remains u...Plants absorb sunlight to power the photochem- ical reactions of photosynthesis, which can potentially damage the photosynthetic machinery. However, the mech- anism that protects chloroplasts from the damage remains unclear. In this work, we demonstrated that rice （Oryza sativa L.） SLAC7 is a generally expressed membrane protein. Loss- of-function of SLAC7 caused continuous damage to the chloroplasts of mutant leaves under normal light conditions. Ion leakage indicators related to leaf damage such as H^O2 and abscisic acid levels were significantly higher in slac7-1 than in the wild type. Consistently, the photosynthesis efficiency and Fv/Fm ratio of slac7-1 were significantly decreased （similar to photoinhibition）. In response to chloroplast damage, slat7- 1 altered its leaf morphology （curled or fused leaf） by the synergy between plant hormones and transcriptional factors to decrease the absorption of light, suggesting that a photoprotection mechanism for chloroplast damage was activated in slac7-1. When grown in dark conditions, slac7-1 displayed a normal phenotype. 5LAC7 under the control of the AtSLAC1 promoter could partially complement thephenotypes of Arabidopsis slacl mutants, indicating a partial conservation of SLAC protein functions. These results suggest that SLAC7 is essential for maintaining the chloroplast stability in rice.展开更多
基金supported by the National Key Research and Development Program of China(grant no.2019YFD1000500)the 2020 Research Program of Sanya Yazhou Bay Science and Technology City(grant no.SKJC-2020-02-002)+2 种基金the Central Public-Interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(grant nos.1630012019009,1630052016005,1630052016006,1630052017021,and 1630052019023)the Central Public-Interest Scientific Institution Basal Research Fund for Innovative Research Team Program of CATAS(grant nos.17CXTD-28 and 1630052017017)the ear-marked fund for Modern Agro-industry Technology Research System(grant no.nycytx-11).
文摘Dear Editor,Generation of heterozygous genomes by hybridization between or within species can help maintain plant diversity and serve as a potential source of new species(Baek et al.,2018).Moreover,genomic heterozygosity is associated with genomic coadaptation,developmental stability,and heterosis.Accurate definition of alleles in haplotypes is necessary to precisely characterize allelic variation controlling agriculturally important traits(Shi et al.,2019).Currently,most released genomes have mosaic assembly of haplotypes due to random selection or collapse of alleles during genome assembly(Shi et al.,2019),which masked allelic variation and functional differentiation of divergent alleles in heterozygous species.
基金funded by the National High Technology Research and Development Program of China (863 Program)the National Program of Transgenic Variety Development of Chinathe National Natural Science Foundation of China
文摘Plants absorb sunlight to power the photochem- ical reactions of photosynthesis, which can potentially damage the photosynthetic machinery. However, the mech- anism that protects chloroplasts from the damage remains unclear. In this work, we demonstrated that rice （Oryza sativa L.） SLAC7 is a generally expressed membrane protein. Loss- of-function of SLAC7 caused continuous damage to the chloroplasts of mutant leaves under normal light conditions. Ion leakage indicators related to leaf damage such as H^O2 and abscisic acid levels were significantly higher in slac7-1 than in the wild type. Consistently, the photosynthesis efficiency and Fv/Fm ratio of slac7-1 were significantly decreased （similar to photoinhibition）. In response to chloroplast damage, slat7- 1 altered its leaf morphology （curled or fused leaf） by the synergy between plant hormones and transcriptional factors to decrease the absorption of light, suggesting that a photoprotection mechanism for chloroplast damage was activated in slac7-1. When grown in dark conditions, slac7-1 displayed a normal phenotype. 5LAC7 under the control of the AtSLAC1 promoter could partially complement thephenotypes of Arabidopsis slacl mutants, indicating a partial conservation of SLAC protein functions. These results suggest that SLAC7 is essential for maintaining the chloroplast stability in rice.
