DNA sequencers have become increasingly important research and diagnostic tools over the past 20 years.In this study,we developed a single-molecule desktop sequencer,GenoCare 1600(GenoCare),which utilizes amplificatio...DNA sequencers have become increasingly important research and diagnostic tools over the past 20 years.In this study,we developed a single-molecule desktop sequencer,GenoCare 1600(GenoCare),which utilizes amplification-free library preparation and two-color sequencing-by-synthesis chemistry,making it more user-friendly compared with previous single-molecule sequencing platforms for clinical use.Using the GenoCare platform,we sequenced an Escherichia coli standard sample and achieved a consensus accuracy exceeding 99.99%.We also evaluated the sequencing performance of this platform in microbial mixtures and coronavirus disease 2019(COVID-19)samples from throat swabs.Our findings indicate that the GenoCare platform allows for microbial quantitation,sensitive identification of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)virus,and accurate detection of virus mutations,as confirmed by Sanger sequencing,demonstrating its remarkable potential in clinical application.展开更多
Receptor-like kinases(RLKs)play key roles in regulating various physiological aspects in plant growth and development.In Arabidopsis thaliana,there are at least 223 leucine-rich repeat(LRR)RLKs.The functions of the ma...Receptor-like kinases(RLKs)play key roles in regulating various physiological aspects in plant growth and development.In Arabidopsis thaliana,there are at least 223 leucine-rich repeat(LRR)RLKs.The functions of the majority of RLKs in the LRR XI subfamily were previously revealed.Only three RLKs were not characterized.Here we report that two independent triple mutants of these RLKs,named ROOT ELONGATION RECEPTOR KINASES(REKs),exhibit increased cell numbers in the root apical meristem and enhanced cell size in the elongation and maturation zones.The promoter activities of a number of Quiescent Center marker genes are significantly up-regulated in the triple mutant.However,the promoter activities of several marker genes known to control root stem cell niche activities are not altered.RNA-seq analysis revealed that a number of cell wall remodeling genes are significantly up-regulated in the triple mutant.Our results suggest that these REKs play key roles in regulating root development likely via negatively regulating the expression of a number of key cell wall remodeling genes.展开更多
文摘DNA sequencers have become increasingly important research and diagnostic tools over the past 20 years.In this study,we developed a single-molecule desktop sequencer,GenoCare 1600(GenoCare),which utilizes amplification-free library preparation and two-color sequencing-by-synthesis chemistry,making it more user-friendly compared with previous single-molecule sequencing platforms for clinical use.Using the GenoCare platform,we sequenced an Escherichia coli standard sample and achieved a consensus accuracy exceeding 99.99%.We also evaluated the sequencing performance of this platform in microbial mixtures and coronavirus disease 2019(COVID-19)samples from throat swabs.Our findings indicate that the GenoCare platform allows for microbial quantitation,sensitive identification of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)virus,and accurate detection of virus mutations,as confirmed by Sanger sequencing,demonstrating its remarkable potential in clinical application.
基金supported by the National Natural Science Foundation of China(31720103902 and 32030005)the 111 Project from the Department of Science and Technology(B16022)。
文摘Receptor-like kinases(RLKs)play key roles in regulating various physiological aspects in plant growth and development.In Arabidopsis thaliana,there are at least 223 leucine-rich repeat(LRR)RLKs.The functions of the majority of RLKs in the LRR XI subfamily were previously revealed.Only three RLKs were not characterized.Here we report that two independent triple mutants of these RLKs,named ROOT ELONGATION RECEPTOR KINASES(REKs),exhibit increased cell numbers in the root apical meristem and enhanced cell size in the elongation and maturation zones.The promoter activities of a number of Quiescent Center marker genes are significantly up-regulated in the triple mutant.However,the promoter activities of several marker genes known to control root stem cell niche activities are not altered.RNA-seq analysis revealed that a number of cell wall remodeling genes are significantly up-regulated in the triple mutant.Our results suggest that these REKs play key roles in regulating root development likely via negatively regulating the expression of a number of key cell wall remodeling genes.
