PANoptosis is a newly identified type of regulated cell death that consists of pyroptosis,apoptosis,and nec roptosis,which simultaneously occur during the pathophysiological process of infectious and inflammatory dise...PANoptosis is a newly identified type of regulated cell death that consists of pyroptosis,apoptosis,and nec roptosis,which simultaneously occur during the pathophysiological process of infectious and inflammatory diseases.Although our previous lite rature mining study suggested that PANoptosis might occur in neuronal ischemia/repe rfusion injury,little experimental research has been reported on the existence of PANoptosis.In this study,we used in vivo and in vitro retinal neuronal models of ischemia/repe rfusion injury to investigate whether PAN optosis-like cell death(simultaneous occurrence of pyroptosis,apo ptosis,and necroptosis)exists in retinal neuronal ischemia/repe rfusion injury.Our results showed that ischemia/repe rfusion injury induced changes in morphological features and protein levels that indicate PANoptosis-like cell death in retinal neurons both in vitro and in vivo.Ischemia/repe rfusion inju ry also significantly upregulated caspase-1,caspase-8,and NLRP3 expression,which are important components of the PANoptosome.These results indicate the existence of PANoptosis-like cell death in ischemia/reperfusion injury of retinal neurons and provide preliminary experimental evidence for future study of this new type of regulated cell death.展开更多
Some scholars have recently developed the concept of PANoptosis in the study of infectious diseases where pyroptosis,apoptosis and necroptosis act in consort in a multimeric protein complex,PANoptosome.This allows all...Some scholars have recently developed the concept of PANoptosis in the study of infectious diseases where pyroptosis,apoptosis and necroptosis act in consort in a multimeric protein complex,PANoptosome.This allows all the components of PANoptosis to be regulated simultaneously.PANoptosis provides a new way to study the regulation of cell death,in that different types of cell death may be regulated at the same time.To test whether PANoptosis exists in diseases other than infectious diseases,we chose cerebral ischemia/reperfusion injury as the research model,collected articles researching cerebral ischemia/reperfusion from three major databases,obtained the original research data from these articles by bibliometrics,data mining and other methods,then integrated and analyzed these data.We selected papers that investigated at least two of the components of PANoptosis to check its occurrence in ischemia/reperfusion.In the cell model simulating ischemic brain injury,pyroptosis,apoptosis and necroptosis occur together and this phenomenon exists widely in different passage cell lines or primary neurons.Pyroptosis,apoptosis and necroptosis also occurred in rat and mouse models of ischemia/reperfusion injury.This confirms that PANoptosis is observed in ischemic brain injury and indicates that PANoptosis can be a target in the regulation of various central nervous system diseases.展开更多
AIM: To develop an image-based objective method to precisely evaluate regional ocular bulbar injection. · METHODS: Six healthy adult volunteers were photographed in four orientations(superior,inferior,nasal and t...AIM: To develop an image-based objective method to precisely evaluate regional ocular bulbar injection. · METHODS: Six healthy adult volunteers were photographed in four orientations(superior,inferior,nasal and temporal sides) with and without stimulating eye drops. Six line segments(covering 30°) were drawn 4mm away from the limbus on each image using ImageJ software. The graph peaks,which were derived from the areas under the line segments and corresponded to the cross-sectional grey-level of the vessels,were analyzed to obtain peak area,peak height/width(PH/PW),and peak numbers. Different-sized areas were selected to calculate the pixels based on the edge-detection algorithm. Also,conjunctival and superficial scleral vessels were analyzed separately. ·RESULTS: This method had a smaller coefficient of variation,especially for PH/PW,in all four orientations. Hyperaemia parameters changed the least after challenging in the superior region. Moreover,95% of the PH/PW ratios were greater than 0.87 in conjunctival vessels and less than 1.00 in superficial scleral vessels. PH/PW significantly increased in conjunctival vessels and changed less in superficial scleral vessels. ·CONCLUSION: A new method of objectively assessing bulbar injection based on ocular surface images was developed. This method can be used to quantify ocular regional injection and to distinguish the superficial scleral and conjunctival vessels.展开更多
Evaluation of flotation behavior, solution measurements, and surface analyses were performed to investigate the effects of chloride ion addition on the sulfidization of cerussite in this study. Micro-flotation tests i...Evaluation of flotation behavior, solution measurements, and surface analyses were performed to investigate the effects of chloride ion addition on the sulfidization of cerussite in this study. Micro-flotation tests indicate that the addition of chloride ions prior to sulfidization can significantly increase the flotation recovery of cerussite, which is attributed to the formation of more lead sulfide species on the mineral surface. Solution measurement results suggest that the addition of chloride ions prior to sulfidization induces the transformation of more sulfide ions from pulp solution onto the mineral surface by the formation of more lead sulfide species. X-ray diffraction and energy-dispersive spectroscopy indicate that more lead sulfide species form on the mineral surface when chloride ions are added prior to sulfidization. These results demonstrate that the addition of chloride ions prior to sulfidization can significantly improve the sulfidization of cerussite, thereby enhancing the flotation performance.展开更多
A modified Miedema model, using interrelationship among the basic properties of elements Ti and H, is employed to calculate the standard enthalpy of formation of titanium hydride TiHx （1≤x≤2）. Based on Debye theor...A modified Miedema model, using interrelationship among the basic properties of elements Ti and H, is employed to calculate the standard enthalpy of formation of titanium hydride TiHx （1≤x≤2）. Based on Debye theories of solid thermal capacity, the vibrational entropy, as well as electronic entropy, is acquired by quantum mechanics and statistic thermodynamics methods, and a new approach is presented to calculate the standard entropy of formation of TiH2. The values of standard enthalpy of formation of TiHx decrease linearly with increase of x. The calculated results of standard enthalpy, entropy, and free energy of formation of TiH2 at 298.16 K are -142.39 kJ/mol, -143.0 J/（mol·K） and -99.75 kJ/mol, respectively, which is consistent with the previously-reported data obtained by either experimental or theoretical calculation methods. The results show that the thermodynamic model for titanium hydride is reasonable.展开更多
A quantitative phase field method of multi-component diffusion-controlled phase transformations coupled with the Kim-Kim-Suzuki model was applied to study the effect of initial particle size distribution （PSD） in 3D...A quantitative phase field method of multi-component diffusion-controlled phase transformations coupled with the Kim-Kim-Suzuki model was applied to study the effect of initial particle size distribution （PSD） in 3D and space distribution in 2D on dissolution of α particles in Ti-6Al-4V alloy below β transus temperature in real time and length scale. The thermodynamic and mobility data were obtained from Thermo-Calc and DICTRA softwares, respectively. The results show that the volume fractions of α particles decay with time as： f =feq ＋ （f0 -feq） exp（-Ktn） for four cases of PSD. The sequence of dissolution kinetics from fast to slow is： uniform PSD, normal PSD, lognormal PSD and bimodal PSD. The space distribution is found to be a major factor affecting the dissolution kinetics and the microstructures. When the distance of the particles is less than critical value, the dissolution rates reduce with the decrease in distance. The Al and V concentration fields around the particles appear more obvious soft impingement.展开更多
We studied the mode-conversion process of terahertz pulses from a planar subwavelength waveguide to a tilted rectangular subwavelength waveguide. An unusual wavefront rotation, which led to an extra conversion t ime, ...We studied the mode-conversion process of terahertz pulses from a planar subwavelength waveguide to a tilted rectangular subwavelength waveguide. An unusual wavefront rotation, which led to an extra conversion t ime, was observed using a time-resolved imaging t echnique. We Simula ted the mode conversion process by a finite-difference time-domain method, and the results agreed well with the experiments. According to the simulations, the conversion time was demonstrated to become longer as the tilt angle or width of the rectangular waveguide increased. This work provides the possibility to optimize the future high-speed communications and t erahertz integrated plat forms.展开更多
基金supported by the National Natural Science Foundation of China,Nos.81772134,81971891,82172196,81571939(ail to KX)the Key Laboratory of Emergency and Trauma(Hainan Medical University)of Ministry of Education,No.KLET-202108(to KX)+1 种基金the Fundamental Research Funds for the Central Universities of Central South University of China,No.2020zzts218(to WTY)Hunan Provincial Innovation Foundation for Postgraduate of China,No.CX20200116(to WTY)。
文摘PANoptosis is a newly identified type of regulated cell death that consists of pyroptosis,apoptosis,and nec roptosis,which simultaneously occur during the pathophysiological process of infectious and inflammatory diseases.Although our previous lite rature mining study suggested that PANoptosis might occur in neuronal ischemia/repe rfusion injury,little experimental research has been reported on the existence of PANoptosis.In this study,we used in vivo and in vitro retinal neuronal models of ischemia/repe rfusion injury to investigate whether PAN optosis-like cell death(simultaneous occurrence of pyroptosis,apo ptosis,and necroptosis)exists in retinal neuronal ischemia/repe rfusion injury.Our results showed that ischemia/repe rfusion injury induced changes in morphological features and protein levels that indicate PANoptosis-like cell death in retinal neurons both in vitro and in vivo.Ischemia/repe rfusion inju ry also significantly upregulated caspase-1,caspase-8,and NLRP3 expression,which are important components of the PANoptosome.These results indicate the existence of PANoptosis-like cell death in ischemia/reperfusion injury of retinal neurons and provide preliminary experimental evidence for future study of this new type of regulated cell death.
基金supported by the National Natural Science Foundation of China,Nos.81772134(to KX),81971891(to KX),82172196(to KX),81571939(to KX)the Fundamental Research Funds for the Central Universities of Central South University of China,No.2020zzts218,(to WTY)Hunan Provincial Innovation Foundation For Postgraduate of China,Nos.CX20200116(to WTY),CX20190139(to LSL).
文摘Some scholars have recently developed the concept of PANoptosis in the study of infectious diseases where pyroptosis,apoptosis and necroptosis act in consort in a multimeric protein complex,PANoptosome.This allows all the components of PANoptosis to be regulated simultaneously.PANoptosis provides a new way to study the regulation of cell death,in that different types of cell death may be regulated at the same time.To test whether PANoptosis exists in diseases other than infectious diseases,we chose cerebral ischemia/reperfusion injury as the research model,collected articles researching cerebral ischemia/reperfusion from three major databases,obtained the original research data from these articles by bibliometrics,data mining and other methods,then integrated and analyzed these data.We selected papers that investigated at least two of the components of PANoptosis to check its occurrence in ischemia/reperfusion.In the cell model simulating ischemic brain injury,pyroptosis,apoptosis and necroptosis occur together and this phenomenon exists widely in different passage cell lines or primary neurons.Pyroptosis,apoptosis and necroptosis also occurred in rat and mouse models of ischemia/reperfusion injury.This confirms that PANoptosis is observed in ischemic brain injury and indicates that PANoptosis can be a target in the regulation of various central nervous system diseases.
基金Supported by National Natural Science Foundation of China(No.81170827)Ministry of Education of China(No.20120171110087)
文摘AIM: To develop an image-based objective method to precisely evaluate regional ocular bulbar injection. · METHODS: Six healthy adult volunteers were photographed in four orientations(superior,inferior,nasal and temporal sides) with and without stimulating eye drops. Six line segments(covering 30°) were drawn 4mm away from the limbus on each image using ImageJ software. The graph peaks,which were derived from the areas under the line segments and corresponded to the cross-sectional grey-level of the vessels,were analyzed to obtain peak area,peak height/width(PH/PW),and peak numbers. Different-sized areas were selected to calculate the pixels based on the edge-detection algorithm. Also,conjunctival and superficial scleral vessels were analyzed separately. ·RESULTS: This method had a smaller coefficient of variation,especially for PH/PW,in all four orientations. Hyperaemia parameters changed the least after challenging in the superior region. Moreover,95% of the PH/PW ratios were greater than 0.87 in conjunctival vessels and less than 1.00 in superficial scleral vessels. PH/PW significantly increased in conjunctival vessels and changed less in superficial scleral vessels. ·CONCLUSION: A new method of objectively assessing bulbar injection based on ocular surface images was developed. This method can be used to quantify ocular regional injection and to distinguish the superficial scleral and conjunctival vessels.
基金financially supported by the National Natural Science Foundation of China (Nos. 51464029 and 51304089)the Analysis and Testing Foundation of Kunming University of Science and Technology (Nos. 20130534 and 20140876)the Academic New Artist Award for Doctoral Post Graduate in Yunnan Province of China (2014)
文摘Evaluation of flotation behavior, solution measurements, and surface analyses were performed to investigate the effects of chloride ion addition on the sulfidization of cerussite in this study. Micro-flotation tests indicate that the addition of chloride ions prior to sulfidization can significantly increase the flotation recovery of cerussite, which is attributed to the formation of more lead sulfide species on the mineral surface. Solution measurement results suggest that the addition of chloride ions prior to sulfidization induces the transformation of more sulfide ions from pulp solution onto the mineral surface by the formation of more lead sulfide species. X-ray diffraction and energy-dispersive spectroscopy indicate that more lead sulfide species form on the mineral surface when chloride ions are added prior to sulfidization. These results demonstrate that the addition of chloride ions prior to sulfidization can significantly improve the sulfidization of cerussite, thereby enhancing the flotation performance.
文摘A modified Miedema model, using interrelationship among the basic properties of elements Ti and H, is employed to calculate the standard enthalpy of formation of titanium hydride TiHx （1≤x≤2）. Based on Debye theories of solid thermal capacity, the vibrational entropy, as well as electronic entropy, is acquired by quantum mechanics and statistic thermodynamics methods, and a new approach is presented to calculate the standard entropy of formation of TiH2. The values of standard enthalpy of formation of TiHx decrease linearly with increase of x. The calculated results of standard enthalpy, entropy, and free energy of formation of TiH2 at 298.16 K are -142.39 kJ/mol, -143.0 J/（mol·K） and -99.75 kJ/mol, respectively, which is consistent with the previously-reported data obtained by either experimental or theoretical calculation methods. The results show that the thermodynamic model for titanium hydride is reasonable.
基金support from the National Natural Science Foundation of China (Nos. 51601078 and 51201147)the National Key R&D Program of China (Grant No. 2016YFB0701302)+1 种基金the Informalization Construction Program of Chinese Academy of Science (INFO-115-B01)Shenyang Supercomputer Centers for the computational resource of Chinese Academy of Science
文摘A quantitative phase field method of multi-component diffusion-controlled phase transformations coupled with the Kim-Kim-Suzuki model was applied to study the effect of initial particle size distribution （PSD） in 3D and space distribution in 2D on dissolution of α particles in Ti-6Al-4V alloy below β transus temperature in real time and length scale. The thermodynamic and mobility data were obtained from Thermo-Calc and DICTRA softwares, respectively. The results show that the volume fractions of α particles decay with time as： f =feq ＋ （f0 -feq） exp（-Ktn） for four cases of PSD. The sequence of dissolution kinetics from fast to slow is： uniform PSD, normal PSD, lognormal PSD and bimodal PSD. The space distribution is found to be a major factor affecting the dissolution kinetics and the microstructures. When the distance of the particles is less than critical value, the dissolution rates reduce with the decrease in distance. The Al and V concentration fields around the particles appear more obvious soft impingement.
基金supported by the National Natural Science Foundation of China (NSFC)(Grant Nos. 61705013 and 61378018)the 111 Project (No. B07013)the Program for Changjiang Scholars and Innovative Research Team in University (No.IRT_13R29).
文摘We studied the mode-conversion process of terahertz pulses from a planar subwavelength waveguide to a tilted rectangular subwavelength waveguide. An unusual wavefront rotation, which led to an extra conversion t ime, was observed using a time-resolved imaging t echnique. We Simula ted the mode conversion process by a finite-difference time-domain method, and the results agreed well with the experiments. According to the simulations, the conversion time was demonstrated to become longer as the tilt angle or width of the rectangular waveguide increased. This work provides the possibility to optimize the future high-speed communications and t erahertz integrated plat forms.
