Studies have shown that C1q/tumor necrosis factor-related protein-6 (CTRP6) can alleviate renal ischemia/reperfusion injury in mice. However, its role in the brain remains poorly understood. To investigate the role of...Studies have shown that C1q/tumor necrosis factor-related protein-6 (CTRP6) can alleviate renal ischemia/reperfusion injury in mice. However, its role in the brain remains poorly understood. To investigate the role of CTRP6 in cerebral ischemia/reperfusion injury associated with diabetes mellitus, a diabetes mellitus mouse model of cerebral ischemia/reperfusion injury was established by occlusion of the middle cerebral artery. To overexpress CTRP6 in the brain, an adeno-associated virus carrying CTRP6 was injected into the lateral ventricle. The result was that oxygen injury and inflammation in brain tissue were clearly attenuated, and the number of neurons was greatly reduced. In vitro experiments showed that CTRP6 knockout exacerbated oxidative damage, inflammatory reaction, and apoptosis in cerebral cortical neurons in high glucose hypoxia-simulated diabetic cerebral ischemia/reperfusion injury. CTRP6 overexpression enhanced the sirtuin-1 signaling pathway in diabetic brains after ischemia/reperfusion injury. To investigate the mechanism underlying these effects, we examined mice with depletion of brain tissue-specific sirtuin-1. CTRP6-like protection was achieved by activating the sirtuin-1 signaling pathway. Taken together, these results indicate that CTRP6 likely attenuates cerebral ischemia/reperfusion injury through activation of the sirtuin-1 signaling pathway.展开更多
Evidence showed that N6-methyladenosine(m^(6)A)modification plays a pivotal role in influencing RNA fate and is strongly associated with cell growth and developmental processes in many species.However,no information r...Evidence showed that N6-methyladenosine(m^(6)A)modification plays a pivotal role in influencing RNA fate and is strongly associated with cell growth and developmental processes in many species.However,no information regarding m^(6)A modification in Eimeria tenella is currently available.In the present study,we surveyed the transcriptome-wide prevalence of m^(6)A in sporulated oocysts and unsporulated oocysts of E.tenella.Methylated RNA immunoprecipitation sequencing(MeRIP-seq)analysis showed that m^(6)A modification was most abundant in the coding sequences,followed by stop codon.There were 3,903 hypermethylated and 3,178 hypomethylated mRNAs in sporulated oocysts compared with unsporulated oocysts.Further joint analysis suggested that m^(6)A modification of the majority of genes was positively correlated with mRNA expression.The mRNA relative expression and m^(6)A level of the selected genes were confirmed by quantitative reverse transcription PCR(RT-qPCR)and MeRIP-qPCR.GO and KEGG analysis indicated that differentially m^(6)A methylated genes(DMMGs)with significant differences in mRNA expression were closely related to processes such as regulation of gene expression,epigenetic,microtubule,autophagy-other and TOR signaling.Moreover,a total of 96 DMMGs without significant differences in mRNA expression showed significant differences at protein level.GO and pathway enrichment analysis of the 96 genes showed that RNA methylation may be involved in cell biosynthesis and metabolism of E.tenella.We firstly present a map of RNA m^(6)A modification in E.tenella,which provides significant insights into developmental biology of E.tenella.展开更多
Genomic prediction(GP)in plant breeding has the potential to predict and identify the best-performing hybrids based on the genotypes of their parental lines.In a GP experiment,34 elite inbred lines were selected to ma...Genomic prediction(GP)in plant breeding has the potential to predict and identify the best-performing hybrids based on the genotypes of their parental lines.In a GP experiment,34 elite inbred lines were selected to make 285 single-cross hybrids in a partial-diallel cross design.These lines represented a mini-core collection of Chinese maize germplasm and comprised 18 inbred lines from the Stiff Stalk heterotic group and 16 inbred lines from the Non-Stiff Stalk heterotic group.The parents were genotyped by sequencing and the 285 hybrids were phenotyped for nine yield and yield-related traits at two locations in the summer sowing area(SUS)and three locations in the spring sowing area(SPS)in the main maizeproducing regions of China.Multiple GP models were employed to assess the accuracy of trait prediction in the hybrids.By ten-fold cross-validation,the prediction accuracies of yield performance of the hybrids estimated by the genomic best linear unbiased prediction(GBLUP)model in SUS and SPS were 0.51 and 0.46,respectively.The prediction accuracies of the remaining yield-related traits estimated with GBLUP ranged from 0.49 to 0.86 and from 0.53 to 0.89 in SUS and SPS,respectively.When additive,dominance,epistasis effects,genotype-by-environment interaction,and multi-trait effects were incorporated into the prediction model,the prediction accuracy of hybrid yield performance was improved.The ratio of training to testing population and size of training population optimal for yield prediction were determined.Multiple prediction models can improve prediction accuracy in hybrid breeding.展开更多
The present study established global brain ischemia using the four-vessel occlusion method.Following three rounds of reperfusion for 30 seconds,and occlusion for 10 seconds,followed by reperfusion for 48 hours,infarct...The present study established global brain ischemia using the four-vessel occlusion method.Following three rounds of reperfusion for 30 seconds,and occlusion for 10 seconds,followed by reperfusion for 48 hours,infarct area,the number of TUNEL-positive cells and Bcl-2 expression were significantly reduced.However,glycogen synthase kinase-3β activity,cortical Bax and caspase-3 expression significantly increased,similar to results following ischemic postconditioning.Our results indicated that ischemic postconditioning may enhance glycogen synthase kinase-3β activity,a downstream molecule of the phosphatase and tensin homolog deleted on chromosome 10/phosphatidylinositol 3-kinase/protein kinase B signaling pathway,which reduces caspase-3 expression to protect the brain against ischemic injury.展开更多
Grain refinement is a well-recognized method to simultaneously increase the strength and ductility of metallic materials.Fabrication of ultrafine-grained metals in bulk using a simple low-cost approach is a long-term ...Grain refinement is a well-recognized method to simultaneously increase the strength and ductility of metallic materials.Fabrication of ultrafine-grained metals in bulk using a simple low-cost approach is a long-term goal for material scientists.In this work,based on the chemical composition of a biomedical Ti-15Zr alloy,a series of novel Ti-15Zr-xCu(x=0,3,5,7 wt.%)alloys were designed and fabricated.The alloys were quenched in the singleβphase region to obtain a martensitic microstructure and deformed in the temperature range of 710–750℃to obtain an ultrafine-grained microstructure through martensite decomposition under thermomechanical coupling conditions.Experimental results showed that Cu alloying could increase the dynamic recrystallization(DRX)nucleation rate due to its role in both refining martensitic lath width and increasing dislocation density.Cu alloying could also suppress grain growth due to the precipitated Ti_(2)Cu particles exerting pinning forces on the grain boundaries.The optimal Cu content in the Ti-15Zr-xCu alloy was determined to be 5 wt.%.After being subjected to a compression leading to a 70%height reduction at 730℃and 1 s^(–1),the grain size of the Ti-15Zr-5Cu alloy was only 180±70 nm.The tensile strength of the as-prepared alloy reached 975±10 MPa,which was 45%higher than that of the conventional Ti-15Zr alloy(673±16 MPa).This increase in strength was achieved without any reduction in ductility.The comprehensive mechanical properties of the ultrafinegrained Ti-15Zr-5Cu alloy are better than that of the Roxolid Ti–Zr alloy currently used for dental implants.展开更多
基金supported by the National Natural Science Foundation of China,Nos.82102295(to WG),82071339(to LG),82001119(to JH),and 81901994(to BZ).
文摘Studies have shown that C1q/tumor necrosis factor-related protein-6 (CTRP6) can alleviate renal ischemia/reperfusion injury in mice. However, its role in the brain remains poorly understood. To investigate the role of CTRP6 in cerebral ischemia/reperfusion injury associated with diabetes mellitus, a diabetes mellitus mouse model of cerebral ischemia/reperfusion injury was established by occlusion of the middle cerebral artery. To overexpress CTRP6 in the brain, an adeno-associated virus carrying CTRP6 was injected into the lateral ventricle. The result was that oxygen injury and inflammation in brain tissue were clearly attenuated, and the number of neurons was greatly reduced. In vitro experiments showed that CTRP6 knockout exacerbated oxidative damage, inflammatory reaction, and apoptosis in cerebral cortical neurons in high glucose hypoxia-simulated diabetic cerebral ischemia/reperfusion injury. CTRP6 overexpression enhanced the sirtuin-1 signaling pathway in diabetic brains after ischemia/reperfusion injury. To investigate the mechanism underlying these effects, we examined mice with depletion of brain tissue-specific sirtuin-1. CTRP6-like protection was achieved by activating the sirtuin-1 signaling pathway. Taken together, these results indicate that CTRP6 likely attenuates cerebral ischemia/reperfusion injury through activation of the sirtuin-1 signaling pathway.
基金supported by the National Natural Science Foundation of China(31902298)the Shanxi Provincial Key Research and Development Program,China(2022ZDYF126)+2 种基金the Fund for Shanxi“1331 Project”,China(20211331-13)the Science and Technology Innovation Program of Shanxi Agricultural University,China(2017YJ10)the Special Research Fund of Shanxi Agricultural University for High-level Talents,China(2021XG001)。
文摘Evidence showed that N6-methyladenosine(m^(6)A)modification plays a pivotal role in influencing RNA fate and is strongly associated with cell growth and developmental processes in many species.However,no information regarding m^(6)A modification in Eimeria tenella is currently available.In the present study,we surveyed the transcriptome-wide prevalence of m^(6)A in sporulated oocysts and unsporulated oocysts of E.tenella.Methylated RNA immunoprecipitation sequencing(MeRIP-seq)analysis showed that m^(6)A modification was most abundant in the coding sequences,followed by stop codon.There were 3,903 hypermethylated and 3,178 hypomethylated mRNAs in sporulated oocysts compared with unsporulated oocysts.Further joint analysis suggested that m^(6)A modification of the majority of genes was positively correlated with mRNA expression.The mRNA relative expression and m^(6)A level of the selected genes were confirmed by quantitative reverse transcription PCR(RT-qPCR)and MeRIP-qPCR.GO and KEGG analysis indicated that differentially m^(6)A methylated genes(DMMGs)with significant differences in mRNA expression were closely related to processes such as regulation of gene expression,epigenetic,microtubule,autophagy-other and TOR signaling.Moreover,a total of 96 DMMGs without significant differences in mRNA expression showed significant differences at protein level.GO and pathway enrichment analysis of the 96 genes showed that RNA methylation may be involved in cell biosynthesis and metabolism of E.tenella.We firstly present a map of RNA m^(6)A modification in E.tenella,which provides significant insights into developmental biology of E.tenella.
基金the National Natural Science Foundation of China(32272049,32261143757)Sustainable Development International Cooperation Program from Bill&Melinda Gates Foundation(2022YFAG1002)+2 种基金the National Key Research and Development Program of China(2020YFE0202300)the Agricultural Science&Technology Innovation Program(CAASZDRW202109)the China Scholarship Council.
文摘Genomic prediction(GP)in plant breeding has the potential to predict and identify the best-performing hybrids based on the genotypes of their parental lines.In a GP experiment,34 elite inbred lines were selected to make 285 single-cross hybrids in a partial-diallel cross design.These lines represented a mini-core collection of Chinese maize germplasm and comprised 18 inbred lines from the Stiff Stalk heterotic group and 16 inbred lines from the Non-Stiff Stalk heterotic group.The parents were genotyped by sequencing and the 285 hybrids were phenotyped for nine yield and yield-related traits at two locations in the summer sowing area(SUS)and three locations in the spring sowing area(SPS)in the main maizeproducing regions of China.Multiple GP models were employed to assess the accuracy of trait prediction in the hybrids.By ten-fold cross-validation,the prediction accuracies of yield performance of the hybrids estimated by the genomic best linear unbiased prediction(GBLUP)model in SUS and SPS were 0.51 and 0.46,respectively.The prediction accuracies of the remaining yield-related traits estimated with GBLUP ranged from 0.49 to 0.86 and from 0.53 to 0.89 in SUS and SPS,respectively.When additive,dominance,epistasis effects,genotype-by-environment interaction,and multi-trait effects were incorporated into the prediction model,the prediction accuracy of hybrid yield performance was improved.The ratio of training to testing population and size of training population optimal for yield prediction were determined.Multiple prediction models can improve prediction accuracy in hybrid breeding.
基金sponsored by the National Natural Science Foundation of China,No.81170768
文摘The present study established global brain ischemia using the four-vessel occlusion method.Following three rounds of reperfusion for 30 seconds,and occlusion for 10 seconds,followed by reperfusion for 48 hours,infarct area,the number of TUNEL-positive cells and Bcl-2 expression were significantly reduced.However,glycogen synthase kinase-3β activity,cortical Bax and caspase-3 expression significantly increased,similar to results following ischemic postconditioning.Our results indicated that ischemic postconditioning may enhance glycogen synthase kinase-3β activity,a downstream molecule of the phosphatase and tensin homolog deleted on chromosome 10/phosphatidylinositol 3-kinase/protein kinase B signaling pathway,which reduces caspase-3 expression to protect the brain against ischemic injury.
基金the National Key Research and Development Program of China(No.2018YFC1106601)the Doctoral Scientific Research Foundation of Liaoning Province(No.2020BS002)。
文摘Grain refinement is a well-recognized method to simultaneously increase the strength and ductility of metallic materials.Fabrication of ultrafine-grained metals in bulk using a simple low-cost approach is a long-term goal for material scientists.In this work,based on the chemical composition of a biomedical Ti-15Zr alloy,a series of novel Ti-15Zr-xCu(x=0,3,5,7 wt.%)alloys were designed and fabricated.The alloys were quenched in the singleβphase region to obtain a martensitic microstructure and deformed in the temperature range of 710–750℃to obtain an ultrafine-grained microstructure through martensite decomposition under thermomechanical coupling conditions.Experimental results showed that Cu alloying could increase the dynamic recrystallization(DRX)nucleation rate due to its role in both refining martensitic lath width and increasing dislocation density.Cu alloying could also suppress grain growth due to the precipitated Ti_(2)Cu particles exerting pinning forces on the grain boundaries.The optimal Cu content in the Ti-15Zr-xCu alloy was determined to be 5 wt.%.After being subjected to a compression leading to a 70%height reduction at 730℃and 1 s^(–1),the grain size of the Ti-15Zr-5Cu alloy was only 180±70 nm.The tensile strength of the as-prepared alloy reached 975±10 MPa,which was 45%higher than that of the conventional Ti-15Zr alloy(673±16 MPa).This increase in strength was achieved without any reduction in ductility.The comprehensive mechanical properties of the ultrafinegrained Ti-15Zr-5Cu alloy are better than that of the Roxolid Ti–Zr alloy currently used for dental implants.
