Extreme low-temperature incidents have become more frequent and severe as climate change intensifies.In HuangHuai-Hai wheat growing area of China,the late spring coldness occurring at the jointing-booting stage(the an...Extreme low-temperature incidents have become more frequent and severe as climate change intensifies.In HuangHuai-Hai wheat growing area of China,the late spring coldness occurring at the jointing-booting stage(the anther interval stage)has resulted in significant yield losses of winter wheat.This study attempts to develop an economical,feasible,and efficient cultivation technique for improving the low-temperature(LT)resistance of wheat by exploring the effects of twice-split phosphorus application(TSPA)on wheat antioxidant characteristics and carbon and nitrogen metabolism physiology under LT treatment at the anther interval stage using Yannong 19 as the experimental material.The treatments consisted of traditional phosphorus application and TSPA,followed by a-4℃ LT treatment and natural temperature(NT)control at the anther interval stage.Our analyses showed that,compared with the traditional application,the TSPA increased the net photosynthetic rate(P_(n)),stomatal conductance(Gs),and transpiration rate(T_(r))of leaves and reduced the intercellular carbon dioxide concentration(C_(i)).The activity of carbon and nitrogen metabolism enzymes in the young wheat spikes was also increased by the TSPA,which promoted the accumulation of soluble sugar(SS),sucrose(SUC),soluble protein(SP),and proline(Pro)in young wheat spike and reduced the toxicity of malondialdehyde(MDA).Due to the improved organic nutrition for reproductive development,the young wheat spikes exhibited enhanced LT resistance,which reduced the sterile spikelet number(SSN)per spike by 11.8%and increased the spikelet setting rate(SSR)and final yield by 6.0 and 8.4%,respectively,compared to the traditional application.The positive effects of split phosphorus application became more pronounced when the LT treatment was prolonged.展开更多
Flexible biosensors with high accuracy and reliable operation in detecting pH and uric acid levels in body fluids are fabricated using well-engineered metaldoped porous carbon as electrode material.The gold nanopartic...Flexible biosensors with high accuracy and reliable operation in detecting pH and uric acid levels in body fluids are fabricated using well-engineered metaldoped porous carbon as electrode material.The gold nanoparticles@N-doped carbon in situ are prepared using wool keratin as both a novel carbon precursor and a stabilizer.The conducting electrode material is fabricated at 500℃ under customized parameters,which mimics A-B type(two different repeating units) polymeric material and displays excellent deprotonation performance(pH sensitivity).The obtained pH sensor exhibits high pH sensitivity of 57 mV/pH unit and insignificant relative standard deviation of 0.088%.Conversely,the composite carbon material with sp^2 structure prepared at 700℃ is doped with nitrogen and gold nanoparticles,which exhibits good conductivity and electrocatalytic activity for uric acid oxidation.The uric acid sensor has linear response over a range of 1-150 μM and a limit of detection 0.1 μM.These results will provide new avenues where biological material will be the best start,which can be useful to target contradictory applications through molecular engineering at mesoscale.展开更多
目的:分析HOXD10基因启动子区甲基化水平与基因表达水平的关联并探索HOXD10与肺腺癌临床病理特征和预后的关系。方法:采用R语言拓展包TCGAbiolinks进行基因差异表达分析和差异甲基化分析,利用肿瘤基因组计划(The Cancer Genome Atlas,TC...目的:分析HOXD10基因启动子区甲基化水平与基因表达水平的关联并探索HOXD10与肺腺癌临床病理特征和预后的关系。方法:采用R语言拓展包TCGAbiolinks进行基因差异表达分析和差异甲基化分析,利用肿瘤基因组计划(The Cancer Genome Atlas,TCGA)项目肺腺癌(lung adenocarcinoma,LUAD)数据集中521例癌症组织和58例癌旁组织的RNA-seq数据分析HOXD10基因差异表达,利用同一数据集中461例癌症组织和32例癌旁组织的甲基化芯片数据分析HOXD10启动子区差异甲基化,并计算启动子区差异甲基化与基因表达水平的相关性,最后分析HOXD10表达与LUAD临床病理特征和预后的关系。结果:在TCGA项目LUAD数据集中,521例癌症组织HOXD10基因的平均表达量为30.4(normalized read counts),低于58例癌旁组织的平均表达量170.8,差异具有统计学意义(P<0.05);在癌症和癌旁组织配对(即来源于同一患者的癌症和癌旁组织组成配对,n=56)时HOXD10基因表达水平的平均差异值为−141.2,差异具有统计学意义(P<0.05)。通过比较461例癌症组织和32例癌旁组织的甲基化芯片数据,鉴定出一个位于HOXD10基因启动子区内的差异甲基化区域,甲基化水平癌症组织高于癌旁组织(Δβ=0.3656,fwer=0)。该区域位于以hg19作为参考基因组的2号染色体176980837~176982342坐标位置,共包含12个甲基化探针,其中cg03918304,cg10364040,cg13217260,cg20649017,cg21591742和cg25371634等6个探针的β值与HOXD10基因的表达情况呈负相关(r<−0.1,P<0.05)。250例肺腺癌患者的完整临床信息显示:HOXD10表达水平与每天抽烟根数和肿瘤大小两个临床病理指标有关(P<0.05),HOXD10低表达组的5年中位生存时间为37.2个月,低于高表达组的59.9个月(P<0.05)。结论:HOXD10基因在TCGA肺腺癌癌症组织中的表达水平明显高于癌旁组织,而甲基化水平明显低于癌旁组织;HOXD10的表达与肺腺癌的临床病理特征有关,是肺腺癌预后的可能影响因素。展开更多
There is increasing evidence shows that either electrical stimulation(ES)or metal ion is an effective way to accelerate ulcerative wound healing.However,less attention is paid to investigating the synergistic effect b...There is increasing evidence shows that either electrical stimulation(ES)or metal ion is an effective way to accelerate ulcerative wound healing.However,less attention is paid to investigating the synergistic effect between them.Herein,we explore the combined effects of ES and multiple metal ions on dia-betic wound healing assisted by a triboelectric nanogenerator(TENG).Firstly,the novel Eggshell@CuFe_(2)O_(4)nanocomposites(NCs)are prepared,which show unique structure and intrinsic antimicrobial properties.Subsequently,the as-prepared nanocomposites are embedded in oxidized starch hydrogel to form a mul-tifunctional composite gel,which is further assembled into a wearable ionic triboelectric nanogenerator(iTENG)patch with polydimethylsiloxane(PDMS).It can convert the mechanical energy produced by a human body motion to electric energy and mediate the sequential release of metal ions(Fe^(2+)/Ca^(2+)/Cu^(2+)),thereby resulting in the“cocktail effect”on impaired tissue.Under their effects,a satisfying healing re-sult in diabetic mouse is identified,which can effectively accelerate wound healing process by relieving inflammation,promoting angiogenesis and collagen deposition.The work puts forward the cocktail effect of electric simulation coupled with the multiple metal ions,and opens up a new perspective in designing iTENG patch towards repair of hard-to-heal wounds.展开更多
基金This work was supported by the Major Science and Technology Projects in Anhui Province,China(202003b06020021)the Natural Science Foundation of Anhui Province,China(2008085QC122)+1 种基金the Postgraduate Quality Engineering Project in Anhui Province,China(2022cxcysj0066)the Special Fund for Anhui Agriculture Research System,China.
文摘Extreme low-temperature incidents have become more frequent and severe as climate change intensifies.In HuangHuai-Hai wheat growing area of China,the late spring coldness occurring at the jointing-booting stage(the anther interval stage)has resulted in significant yield losses of winter wheat.This study attempts to develop an economical,feasible,and efficient cultivation technique for improving the low-temperature(LT)resistance of wheat by exploring the effects of twice-split phosphorus application(TSPA)on wheat antioxidant characteristics and carbon and nitrogen metabolism physiology under LT treatment at the anther interval stage using Yannong 19 as the experimental material.The treatments consisted of traditional phosphorus application and TSPA,followed by a-4℃ LT treatment and natural temperature(NT)control at the anther interval stage.Our analyses showed that,compared with the traditional application,the TSPA increased the net photosynthetic rate(P_(n)),stomatal conductance(Gs),and transpiration rate(T_(r))of leaves and reduced the intercellular carbon dioxide concentration(C_(i)).The activity of carbon and nitrogen metabolism enzymes in the young wheat spikes was also increased by the TSPA,which promoted the accumulation of soluble sugar(SS),sucrose(SUC),soluble protein(SP),and proline(Pro)in young wheat spike and reduced the toxicity of malondialdehyde(MDA).Due to the improved organic nutrition for reproductive development,the young wheat spikes exhibited enhanced LT resistance,which reduced the sterile spikelet number(SSN)per spike by 11.8%and increased the spikelet setting rate(SSR)and final yield by 6.0 and 8.4%,respectively,compared to the traditional application.The positive effects of split phosphorus application became more pronounced when the LT treatment was prolonged.
基金supported by the National Natural Science Foundation of China(Grant Nos.51502253,U1405226,21503175,51773171,and 21705135)Natural Science Foundation of Guangdong Province(Grant No.2016A030310369)+5 种基金Natural Science Foundation of Fujian Province(Grant No.2017J01104)the Fundamental Research Funds for the Central Universities of China(Grant Nos.20720160127 and 20720180013)Doctoral Fund of the Ministry of Education(Grant No.20130121110018)NUS Ac RF Tier 1(Grant No.R-144-000-367-112)the “111” Project(Grant No.B16029)the 1000 Talents Program funding from the Xiamen University。
文摘Flexible biosensors with high accuracy and reliable operation in detecting pH and uric acid levels in body fluids are fabricated using well-engineered metaldoped porous carbon as electrode material.The gold nanoparticles@N-doped carbon in situ are prepared using wool keratin as both a novel carbon precursor and a stabilizer.The conducting electrode material is fabricated at 500℃ under customized parameters,which mimics A-B type(two different repeating units) polymeric material and displays excellent deprotonation performance(pH sensitivity).The obtained pH sensor exhibits high pH sensitivity of 57 mV/pH unit and insignificant relative standard deviation of 0.088%.Conversely,the composite carbon material with sp^2 structure prepared at 700℃ is doped with nitrogen and gold nanoparticles,which exhibits good conductivity and electrocatalytic activity for uric acid oxidation.The uric acid sensor has linear response over a range of 1-150 μM and a limit of detection 0.1 μM.These results will provide new avenues where biological material will be the best start,which can be useful to target contradictory applications through molecular engineering at mesoscale.
基金supported financially by the Natural Science Foundation of Shanxi Province Grants(No.202203021211157)Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering(No.2023SX-FR006)The Science and Technology Major Special Plan Project“Reveal the Tile”of Quanzhou Province(No.2022NZ1).
文摘There is increasing evidence shows that either electrical stimulation(ES)or metal ion is an effective way to accelerate ulcerative wound healing.However,less attention is paid to investigating the synergistic effect between them.Herein,we explore the combined effects of ES and multiple metal ions on dia-betic wound healing assisted by a triboelectric nanogenerator(TENG).Firstly,the novel Eggshell@CuFe_(2)O_(4)nanocomposites(NCs)are prepared,which show unique structure and intrinsic antimicrobial properties.Subsequently,the as-prepared nanocomposites are embedded in oxidized starch hydrogel to form a mul-tifunctional composite gel,which is further assembled into a wearable ionic triboelectric nanogenerator(iTENG)patch with polydimethylsiloxane(PDMS).It can convert the mechanical energy produced by a human body motion to electric energy and mediate the sequential release of metal ions(Fe^(2+)/Ca^(2+)/Cu^(2+)),thereby resulting in the“cocktail effect”on impaired tissue.Under their effects,a satisfying healing re-sult in diabetic mouse is identified,which can effectively accelerate wound healing process by relieving inflammation,promoting angiogenesis and collagen deposition.The work puts forward the cocktail effect of electric simulation coupled with the multiple metal ions,and opens up a new perspective in designing iTENG patch towards repair of hard-to-heal wounds.
