A total of five pairs of diploidotriploid twin-seedlings （a diploid seedling and a triploid seedling emerged from a grain） were selected out from 4500 pairs of seedlings from SARII-628, a twin-seedling rice line. SS...A total of five pairs of diploidotriploid twin-seedlings （a diploid seedling and a triploid seedling emerged from a grain） were selected out from 4500 pairs of seedlings from SARII-628, a twin-seedling rice line. SSR analysis indicated that no difference between the diploid seedling and corresponding triploid seedling in a twin-seedling was found at the 310 loci, indicating that there was no obvious change in DNA primary structure. A modified AFLP technique ＇MSAP （methylation-sensitive AFLP）＇ was used to analyze methylation mutation. Although no methylation mutation was noted among the five diploids, 29 methylation mutation loci were found from the corresponding triploids. This suggested that methylation mutation happened rapidly on Mogeneration after natural homologous triploidization. The mutations were classified into 10 types, including 3 increased types, 3 decreased types and 4 undecided types of methylation-degrees. The bands of 22 loci were sequenced and then those sequences were searched through website. The result showed that the methylation mutation involved into the whole rice genome and the 12 pairs of chromosomes. The mutation trend was site-related and there were different mutation loci for different triploids, which foretold that SARII-628 would have different evolution fates after natural homologous triploidization.展开更多
The mechanism of early generation stability （EGS） in rice was studied via genetic analysis. Three types of crosses were made, namely between EGS varieties, EGS and conventional rice variety, and conventional rice va...The mechanism of early generation stability （EGS） in rice was studied via genetic analysis. Three types of crosses were made, namely between EGS varieties, EGS and conventional rice variety, and conventional rice varieties. The genetic analysis was based on the stable lines in F2 population. The stable lines may appear from some combinations of EGS rice crossing with each other and EGS rice crossing with conventional varieties at different frequencies, but stable lines didn＇t appear in conventional varieties crossing with conventional varieties. Genetic analysis results indicated that the EGS phenomena should just exist in special rice materials, and the frequency of stable lines was closely related to the EGS traits of parents. The EGS traits were neither qualitative nor quantitative traits, and they were controlled by neither dominant genes nor recessive genes. The EGS traits might be inherited by F1 single plant, and the traits of F3 and F4 were corresponded to those of F2 population, i.e. F3 and F4 lines derived from non-segregating F2 showed uniform agronomic traits, and those from segregating F2.did not. The agronomic traits of EGS lines were consistent with those of F1 single plant. On the other hand, when EGS lines occurred, the segregating lines in Mendelian manner were also observed in all F2 population of the same combination. It was suggested that the reason why the stable strains occurred might be a special factor to control （open/close） gene at the beginning of cell division in zygote, resulting in closing mitosis and opening somatic reduction. The somatic reduction of zygote resulted in recombination and homozygosity forming in F1 single plant, and some lines with uniform agronomic traits were observed in some lines of F2 population.展开更多
The effect of light on flavonoids biosynthesis in red rice Rdh was studied. The panicles of red rice Rdh produced colorless caryopses after darkness treatment; and these colorless caryopses displayed bright-red after ...The effect of light on flavonoids biosynthesis in red rice Rdh was studied. The panicles of red rice Rdh produced colorless caryopses after darkness treatment; and these colorless caryopses displayed bright-red after vanillin treatment, but did not display red color after light inducing for 15 days, suggesting that red rice Rdh could produce leucoanthocyanidin, but could not produce polyproanthocyanidins in darkness. Histological study revealed that the aleurone layers of Rdh colorless caryopses displayed bright-red after vanillin assay, but the pericarp and seed coat layers did not display color change, which indicated that the aleurone layers could accumulate precursors of polyproanthocyanidins in darkness, but the pericarp and seed coat could not. Additionally, color ofRdh caryopses changed from green in immaturity to red in maturity, and the green caryopses changed color from green to red gradually indoor for 7 days after harvest, suggesting that leucoanthocyanidins could synthesize polyproanthocyanidins. It was concluded that light was necessary for red pigment biosynthesis in red rice Rdh, leucoanthocyanidins biosyntheses in the aleurone layers did not need light, leucoanthocyanidins biosynthesis in pericarp and seed coat needed light inducing, the effect of leucoanthocyanidin biosynthesis in Rdh to light had tissue specificity.展开更多
Different culture explants, including anther, young panicle, young embryo, and mature embryo, from 19 rice varieties were used for callus induction and green plantlet differentiation. The culture efficiency differed s...Different culture explants, including anther, young panicle, young embryo, and mature embryo, from 19 rice varieties were used for callus induction and green plantlet differentiation. The culture efficiency differed significantly among the four types of explants, and varied from genotype to genotype. Callus induction frequency presented significantly positive correlation each between anther and young panicle, anther and mature embryo, and young panicle and young embryo. Green plantlet differentiation showed no relationship between different types of explants. In addition, no relationship was found between callus induction frequency and green plantlet differentiation frequency.展开更多
DNA methylation is one of the epigenetic phenomena which can be transferred to the offspring by cell division in the evolution of organisms. The epigenetic regulation accompanied by gene expression can be found direct...DNA methylation is one of the epigenetic phenomena which can be transferred to the offspring by cell division in the evolution of organisms. The epigenetic regulation accompanied by gene expression can be found directly in the phenotype of haploidy plants. DNA cytosine methylation at the 5＇-CpCpGpG sites of haploid, Shuhui 527, Shuhui 363 and their hybrids was analyzed by methylation sensitive amplification polymorphism （MSAP） method. There were 765 DNA methylated sites detected and the methylation level was lower in hybrids than parents. Meanwhile, the different bands between hybrids and parents were analyzed and two types of methylated sites were detected, of which one inherited from haploid, and the other did not. The biological functions of genes related to methylated sites involved in cell structure, metabolize and response factor. Therefore, DNA methylated modifications can activate and silence the genes and play an important role in plant growth, development and evolution.展开更多
A comparative study on chloroplast ultrastructure and light harvesting complex of photosystem Ⅱ （LHC Ⅱ） was conducted between a new rice mutant （W2555） and its wild type （WT）. The chloroplasts of W2555 had les...A comparative study on chloroplast ultrastructure and light harvesting complex of photosystem Ⅱ （LHC Ⅱ） was conducted between a new rice mutant （W2555） and its wild type （WT）. The chloroplasts of W2555 had less thylakoids and grana stacks compared with the wild type. There was no significant change in the composition of LHC Ⅱ polypeptide in W2555, while a decline had been noted in LHC Ⅱ content. Northern blot analysis with a specific cab gene probe showed no appreciable difference in the LHC Ⅱ mRNA level between the W2555and its wild type. The precursors of chlorophyll synthesis, 6-aminolevulinic acid （ALA） and porphobilinogen （PBG） were over accumulated in W2555, but the other precursors were all decreased. These results indicated that the decreased level of LHC Ⅱ in the mutant W2555 was attributed to the change of cab gene transcription, but a blockage in chlorophyll biosynthesis due to the formation of uroporphyrinogen Ⅲ （Urogen Ⅲ）.展开更多
A spontaneous mutation, tentatively named d63, was derived from the twin-seedling progenies of rice crossed by diploid SARIII and Minghui 63. Compared with wild-type plants, the d63 mutant showed multiple abnormal phe...A spontaneous mutation, tentatively named d63, was derived from the twin-seedling progenies of rice crossed by diploid SARIII and Minghui 63. Compared with wild-type plants, the d63 mutant showed multiple abnormal phenotypes, such as dwarfism, more tillers, smaller flag leaf and reduced seed-setting rate and 1000-grain weight. In this study, two F2 populations were developed by crossing between d63 and Nipponbare, d63 and 93-11. Genetic analysis indicated that d63 was controlled by a single recessive gene, which was located on the short arm of chromosome 8, within the genetic distance of 0.40 cM from RM22195. Hence, D63 might be a new gene as there are no dwarf genes reported on the short arm of chromosome 8.展开更多
A spontaneous mutant with multiple stigmas （msf） was found in an indica rice line 466. The mst mutant exhibits normal at the vegetative development stage and produces normal inflorescence structures. The difference ...A spontaneous mutant with multiple stigmas （msf） was found in an indica rice line 466. The mst mutant exhibits normal at the vegetative development stage and produces normal inflorescence structures. The difference between the mutant and the wild type was observed when the stamen primordium began to develop. In the mst florets, palea and lemma opened, Iodicules were homeotically transformed into palea/lemma-like structures, and stamens were homeotically transformed into carpel-like structures. It looked like multiple stigmas being full of the whole floret. The phenotypic changes of rest were very similar to that of B-like mutant spwl. Compared with other mutants with pistillate morphologies, the severe mstflorets showed that the inner three floral organs were completely changed into palea/lemma-like structures. Moreover, the mutant was female sterile. Occasionally, with the changing environment, one or two stamens were fertile. Genetic analysis indicated that the mutant traits were controlled by a single recessive gene.展开更多
文摘A total of five pairs of diploidotriploid twin-seedlings （a diploid seedling and a triploid seedling emerged from a grain） were selected out from 4500 pairs of seedlings from SARII-628, a twin-seedling rice line. SSR analysis indicated that no difference between the diploid seedling and corresponding triploid seedling in a twin-seedling was found at the 310 loci, indicating that there was no obvious change in DNA primary structure. A modified AFLP technique ＇MSAP （methylation-sensitive AFLP）＇ was used to analyze methylation mutation. Although no methylation mutation was noted among the five diploids, 29 methylation mutation loci were found from the corresponding triploids. This suggested that methylation mutation happened rapidly on Mogeneration after natural homologous triploidization. The mutations were classified into 10 types, including 3 increased types, 3 decreased types and 4 undecided types of methylation-degrees. The bands of 22 loci were sequenced and then those sequences were searched through website. The result showed that the methylation mutation involved into the whole rice genome and the 12 pairs of chromosomes. The mutation trend was site-related and there were different mutation loci for different triploids, which foretold that SARII-628 would have different evolution fates after natural homologous triploidization.
基金China Natural Science Foundation(30001037) Youth Foundation of Sichuan Province,China.
文摘The mechanism of early generation stability （EGS） in rice was studied via genetic analysis. Three types of crosses were made, namely between EGS varieties, EGS and conventional rice variety, and conventional rice varieties. The genetic analysis was based on the stable lines in F2 population. The stable lines may appear from some combinations of EGS rice crossing with each other and EGS rice crossing with conventional varieties at different frequencies, but stable lines didn＇t appear in conventional varieties crossing with conventional varieties. Genetic analysis results indicated that the EGS phenomena should just exist in special rice materials, and the frequency of stable lines was closely related to the EGS traits of parents. The EGS traits were neither qualitative nor quantitative traits, and they were controlled by neither dominant genes nor recessive genes. The EGS traits might be inherited by F1 single plant, and the traits of F3 and F4 were corresponded to those of F2 population, i.e. F3 and F4 lines derived from non-segregating F2 showed uniform agronomic traits, and those from segregating F2.did not. The agronomic traits of EGS lines were consistent with those of F1 single plant. On the other hand, when EGS lines occurred, the segregating lines in Mendelian manner were also observed in all F2 population of the same combination. It was suggested that the reason why the stable strains occurred might be a special factor to control （open/close） gene at the beginning of cell division in zygote, resulting in closing mitosis and opening somatic reduction. The somatic reduction of zygote resulted in recombination and homozygosity forming in F1 single plant, and some lines with uniform agronomic traits were observed in some lines of F2 population.
基金supported by the program for Changjiang Scholars and Innovative Research Teams in Universties,China (IRT0453)
文摘The effect of light on flavonoids biosynthesis in red rice Rdh was studied. The panicles of red rice Rdh produced colorless caryopses after darkness treatment; and these colorless caryopses displayed bright-red after vanillin treatment, but did not display red color after light inducing for 15 days, suggesting that red rice Rdh could produce leucoanthocyanidin, but could not produce polyproanthocyanidins in darkness. Histological study revealed that the aleurone layers of Rdh colorless caryopses displayed bright-red after vanillin assay, but the pericarp and seed coat layers did not display color change, which indicated that the aleurone layers could accumulate precursors of polyproanthocyanidins in darkness, but the pericarp and seed coat could not. Additionally, color ofRdh caryopses changed from green in immaturity to red in maturity, and the green caryopses changed color from green to red gradually indoor for 7 days after harvest, suggesting that leucoanthocyanidins could synthesize polyproanthocyanidins. It was concluded that light was necessary for red pigment biosynthesis in red rice Rdh, leucoanthocyanidins biosyntheses in the aleurone layers did not need light, leucoanthocyanidins biosynthesis in pericarp and seed coat needed light inducing, the effect of leucoanthocyanidin biosynthesis in Rdh to light had tissue specificity.
文摘Different culture explants, including anther, young panicle, young embryo, and mature embryo, from 19 rice varieties were used for callus induction and green plantlet differentiation. The culture efficiency differed significantly among the four types of explants, and varied from genotype to genotype. Callus induction frequency presented significantly positive correlation each between anther and young panicle, anther and mature embryo, and young panicle and young embryo. Green plantlet differentiation showed no relationship between different types of explants. In addition, no relationship was found between callus induction frequency and green plantlet differentiation frequency.
基金supported by the National Natural Science Foundation of China (Grant Nos. 30771157and 30971618)Science and Technology in Sichuan Province,China (Grant No. 2008JY0094)
文摘DNA methylation is one of the epigenetic phenomena which can be transferred to the offspring by cell division in the evolution of organisms. The epigenetic regulation accompanied by gene expression can be found directly in the phenotype of haploidy plants. DNA cytosine methylation at the 5＇-CpCpGpG sites of haploid, Shuhui 527, Shuhui 363 and their hybrids was analyzed by methylation sensitive amplification polymorphism （MSAP） method. There were 765 DNA methylated sites detected and the methylation level was lower in hybrids than parents. Meanwhile, the different bands between hybrids and parents were analyzed and two types of methylated sites were detected, of which one inherited from haploid, and the other did not. The biological functions of genes related to methylated sites involved in cell structure, metabolize and response factor. Therefore, DNA methylated modifications can activate and silence the genes and play an important role in plant growth, development and evolution.
文摘A comparative study on chloroplast ultrastructure and light harvesting complex of photosystem Ⅱ （LHC Ⅱ） was conducted between a new rice mutant （W2555） and its wild type （WT）. The chloroplasts of W2555 had less thylakoids and grana stacks compared with the wild type. There was no significant change in the composition of LHC Ⅱ polypeptide in W2555, while a decline had been noted in LHC Ⅱ content. Northern blot analysis with a specific cab gene probe showed no appreciable difference in the LHC Ⅱ mRNA level between the W2555and its wild type. The precursors of chlorophyll synthesis, 6-aminolevulinic acid （ALA） and porphobilinogen （PBG） were over accumulated in W2555, but the other precursors were all decreased. These results indicated that the decreased level of LHC Ⅱ in the mutant W2555 was attributed to the change of cab gene transcription, but a blockage in chlorophyll biosynthesis due to the formation of uroporphyrinogen Ⅲ （Urogen Ⅲ）.
文摘A spontaneous mutation, tentatively named d63, was derived from the twin-seedling progenies of rice crossed by diploid SARIII and Minghui 63. Compared with wild-type plants, the d63 mutant showed multiple abnormal phenotypes, such as dwarfism, more tillers, smaller flag leaf and reduced seed-setting rate and 1000-grain weight. In this study, two F2 populations were developed by crossing between d63 and Nipponbare, d63 and 93-11. Genetic analysis indicated that d63 was controlled by a single recessive gene, which was located on the short arm of chromosome 8, within the genetic distance of 0.40 cM from RM22195. Hence, D63 might be a new gene as there are no dwarf genes reported on the short arm of chromosome 8.
基金supported by the Program for Changjiang Scholars and Innovative Research Team in University of China (Grant No. IRTO453)
文摘A spontaneous mutant with multiple stigmas （msf） was found in an indica rice line 466. The mst mutant exhibits normal at the vegetative development stage and produces normal inflorescence structures. The difference between the mutant and the wild type was observed when the stamen primordium began to develop. In the mst florets, palea and lemma opened, Iodicules were homeotically transformed into palea/lemma-like structures, and stamens were homeotically transformed into carpel-like structures. It looked like multiple stigmas being full of the whole floret. The phenotypic changes of rest were very similar to that of B-like mutant spwl. Compared with other mutants with pistillate morphologies, the severe mstflorets showed that the inner three floral organs were completely changed into palea/lemma-like structures. Moreover, the mutant was female sterile. Occasionally, with the changing environment, one or two stamens were fertile. Genetic analysis indicated that the mutant traits were controlled by a single recessive gene.
