The interaction mechanism between human serum albumin(HSA) and 1-phenyl-3(fluorenone-2-yl)-5-(9-ethylcarbazole-3-yl)-2- pyrazoline(PFEP) was investigated by fluorescence and absorption titration techniques in combinat...The interaction mechanism between human serum albumin(HSA) and 1-phenyl-3(fluorenone-2-yl)-5-(9-ethylcarbazole-3-yl)-2- pyrazoline(PFEP) was investigated by fluorescence and absorption titration techniques in combination with molecular modeling method.Stern-Volmer plots at different temperatures proved that PFEP could quench the intrinsic fluorescence of HSA attributed to a static quenching procedure.The association constants were calculated in the range of 1×10~5-8×10~5mol^(-1) at different pH conditions,a...展开更多
基金supported by the National Natural Science Foundation of China(No.20875059)
文摘The interaction mechanism between human serum albumin(HSA) and 1-phenyl-3(fluorenone-2-yl)-5-(9-ethylcarbazole-3-yl)-2- pyrazoline(PFEP) was investigated by fluorescence and absorption titration techniques in combination with molecular modeling method.Stern-Volmer plots at different temperatures proved that PFEP could quench the intrinsic fluorescence of HSA attributed to a static quenching procedure.The association constants were calculated in the range of 1×10~5-8×10~5mol^(-1) at different pH conditions,a...
