This study aimed to explore the effect of Bifidobacterium animalis F1-7 on the improvement of atherosclerotic inflammation.Arteriosclerosis model ApoE^(-/-)mice were orally administered with B.animalis F1-7 for 12 wee...This study aimed to explore the effect of Bifidobacterium animalis F1-7 on the improvement of atherosclerotic inflammation.Arteriosclerosis model ApoE^(-/-)mice were orally administered with B.animalis F1-7 for 12 weeks.The probiotic intervention reduced the plaque areas in aorta and the accumulation of macrophages,and downregulated the expression of toll-like receptor 4(TLR4)/nuclear factorκB(NF-κB)pathway to reduce the levels of inflammatory factors.The widely-targeted metabolomics analysis showed that acetyl-L-carnitine(ALC)in the intestine of atherosclerotic mice was significantly increased after B.animalis F1-7 intervention.Correlation analysis proved that ALC was associated with atherosclerotic inflammatory response.By using oxidized low density lipoprotein induced macrophage foam cells,we further verified that ALC could reduce lipid accumulation and inflammatory response in foam cells by downregulating the TLR4/NF-κB pathway.Finally,our results revealed that B.animalis F1-7 upregulated the metabolite ALC to downregulate the inflammatory responses,leading to the reduction of plaque accumulation of atherosclerosis.展开更多
目的探讨1.2 mm×35 mm TWLB注射器(薄壁长斜面刃角)针头引导下经腹壁双J管置入在完全腹腔镜下回肠新膀胱术中的应用。方法回顾性分析2020年12月-2022年1月昆明医科大学第二附属医院5例行完全腹腔镜下回肠新膀胱术患者的资料。在腹...目的探讨1.2 mm×35 mm TWLB注射器(薄壁长斜面刃角)针头引导下经腹壁双J管置入在完全腹腔镜下回肠新膀胱术中的应用。方法回顾性分析2020年12月-2022年1月昆明医科大学第二附属医院5例行完全腹腔镜下回肠新膀胱术患者的资料。在腹腔镜及肉眼直视下,取耻骨联合上3 cm作为穿刺点,刺入TWLB注射器针头建立通道,通过针头在导丝引导下行双J管置入。结果5例患者均在1.2 mm×35 mm TWLB注射器针头引导下成功置入输尿管支架,用时2~4 min,平均3 min。结论在1.2 mm×35 mm TWLB注射器针头引导下,经腹壁双J管置入在完全腹腔镜下回肠新膀胱术中可以保留原有操作通道、不破坏气腹,并且效果确切、简单可行、取材易得、安全有效并缩短手术时间,是完全腹腔镜下回肠新膀胱术中一种新的辅助手段。展开更多
Thefilter-x least mean square(FxLMS)algorithm is widely used in active noise control(ANC)systems.However,because the algorithm is a feedback control algorithm based on the minimization of the error signal variance to ...Thefilter-x least mean square(FxLMS)algorithm is widely used in active noise control(ANC)systems.However,because the algorithm is a feedback control algorithm based on the minimization of the error signal variance to update thefilter coefficients,it has a certain delay,usually has a slow convergence speed,and the system response time is long and easily affected by the learning rate leading to the lack of system stability,which often fails to achieve the desired control effect in practice.In this paper,we propose an active control algorithm with near-est-neighbor trap structure and neural network feedback mechanism to reduce the coefficient update time of the FxLMS algorithm and use the neural network feedback mechanism to realize the parameter update,which is called NNR-BPFxLMS algorithm.In the paper,the schematic diagram of the feedback control is given,and the performance of the algorithm is analyzed.Under various noise conditions,it is shown by simulation and experiment that the NNR-BPFxLMS algorithm has the following three advantages:in terms of performance,it has higher noise reduction under the same number of sampling points,i.e.,it has faster convergence speed,and by computer simulation and sound pipe experiment,for simple ideal line spectrum noise,compared with the convergence speed of NNR-BPFxLMS is improved by more than 95%compared with FxLMS algorithm,and the convergence speed of real noise is also improved by more than 70%.In terms of stability,NNR-BPFxLMS is insensitive to step size changes.In terms of tracking performance,its algorithm responds quickly to sudden changes in the noise spectrum and can cope with the complex control requirements of sudden changes in the noise spectrum.展开更多
Collective cancer cell migration(CCCM)and epithelial-to-mesenchymal transition(EMT)play key roles in metastasis.This study reports that the colorectal carcinoma cell line LIM1863 is useful for the study of CCCM and EM...Collective cancer cell migration(CCCM)and epithelial-to-mesenchymal transition(EMT)play key roles in metastasis.This study reports that the colorectal carcinoma cell line LIM1863 is useful for the study of CCCM and EMT.Methods:Hematoxylin and eosin staining,scanning electron microscopy,transmission electron microscopy,and western blot analysis were performed.Results:LIM1863 automatically grew as spheroids in suspension and had important typical epithelial properties,including several layers of cells arranged around a central lumen,apical-basal polarity,and types of cell-cell junctions.Treatment with a combination of both TGF beta 1 and TNF alpha induced definite and distinct EMT,a spheroid changing phenotype to form a monolayer high-confluent patch without lumen,without polarity.Spontaneous CCCM occurred in spheroids.Flat EMT cells adhered to the base of a dish,exhibited persistent movement as a cluster of cells,and then shed,resulting in a cluster.All cells from one cluster undergoing CCCM died.Otherwise,all cells undergoing EMT disappeared and almost all cells located in the cell reservoir survived and proliferated.Conclusion:LIM1863 is an excellent cell line to study CCCM and EMT.The group of heterogeneous cells undergoing CCCM behaves like a supracellular unit.展开更多
Against the existential and ubiquitous threat of climate changes to the environment,human health,and economic well-being,the proliferation of carbon neutrality from both government and private sector recently prevails...Against the existential and ubiquitous threat of climate changes to the environment,human health,and economic well-being,the proliferation of carbon neutrality from both government and private sector recently prevails in order to strengthen preparedness for the future global crisis.More than 70 countries and regions have pledged to achieve the goal of carbon neutrality by 2050(ICAP,2020).While the momentum toward adopting carbon neutrality targets continue to build,a growing body of countries and subnational governments move toward carbon pricing through rolling out,speeding-up,or strengthening the construct of emission trading scheme(ETS)and carbon financial market.Notwithstanding the laudable objectives of ETS to enable emission reduction,the potential of ETS is still largely untapped,largely stemming from poor financial innovation.This special issue is,therefore,aimed at promoting research on the carbon financial innovations,financial derivatives services,the use of Fintech and the optimization on architectures of the ETS such that ETSs could drive the transformation toward low-carbon development path and the achievement of Carbon Neutrality targets.展开更多
基金supported by Shandong Taishan industry leading talent project(LJNY202101)the National Key R&D of China(2018YFC0311201)。
文摘This study aimed to explore the effect of Bifidobacterium animalis F1-7 on the improvement of atherosclerotic inflammation.Arteriosclerosis model ApoE^(-/-)mice were orally administered with B.animalis F1-7 for 12 weeks.The probiotic intervention reduced the plaque areas in aorta and the accumulation of macrophages,and downregulated the expression of toll-like receptor 4(TLR4)/nuclear factorκB(NF-κB)pathway to reduce the levels of inflammatory factors.The widely-targeted metabolomics analysis showed that acetyl-L-carnitine(ALC)in the intestine of atherosclerotic mice was significantly increased after B.animalis F1-7 intervention.Correlation analysis proved that ALC was associated with atherosclerotic inflammatory response.By using oxidized low density lipoprotein induced macrophage foam cells,we further verified that ALC could reduce lipid accumulation and inflammatory response in foam cells by downregulating the TLR4/NF-κB pathway.Finally,our results revealed that B.animalis F1-7 upregulated the metabolite ALC to downregulate the inflammatory responses,leading to the reduction of plaque accumulation of atherosclerosis.
文摘目的探讨1.2 mm×35 mm TWLB注射器(薄壁长斜面刃角)针头引导下经腹壁双J管置入在完全腹腔镜下回肠新膀胱术中的应用。方法回顾性分析2020年12月-2022年1月昆明医科大学第二附属医院5例行完全腹腔镜下回肠新膀胱术患者的资料。在腹腔镜及肉眼直视下,取耻骨联合上3 cm作为穿刺点,刺入TWLB注射器针头建立通道,通过针头在导丝引导下行双J管置入。结果5例患者均在1.2 mm×35 mm TWLB注射器针头引导下成功置入输尿管支架,用时2~4 min,平均3 min。结论在1.2 mm×35 mm TWLB注射器针头引导下,经腹壁双J管置入在完全腹腔镜下回肠新膀胱术中可以保留原有操作通道、不破坏气腹,并且效果确切、简单可行、取材易得、安全有效并缩短手术时间,是完全腹腔镜下回肠新膀胱术中一种新的辅助手段。
基金This work was supported by the National Key R&D Program of China(Grant No.2020YFA040070).
文摘Thefilter-x least mean square(FxLMS)algorithm is widely used in active noise control(ANC)systems.However,because the algorithm is a feedback control algorithm based on the minimization of the error signal variance to update thefilter coefficients,it has a certain delay,usually has a slow convergence speed,and the system response time is long and easily affected by the learning rate leading to the lack of system stability,which often fails to achieve the desired control effect in practice.In this paper,we propose an active control algorithm with near-est-neighbor trap structure and neural network feedback mechanism to reduce the coefficient update time of the FxLMS algorithm and use the neural network feedback mechanism to realize the parameter update,which is called NNR-BPFxLMS algorithm.In the paper,the schematic diagram of the feedback control is given,and the performance of the algorithm is analyzed.Under various noise conditions,it is shown by simulation and experiment that the NNR-BPFxLMS algorithm has the following three advantages:in terms of performance,it has higher noise reduction under the same number of sampling points,i.e.,it has faster convergence speed,and by computer simulation and sound pipe experiment,for simple ideal line spectrum noise,compared with the convergence speed of NNR-BPFxLMS is improved by more than 95%compared with FxLMS algorithm,and the convergence speed of real noise is also improved by more than 70%.In terms of stability,NNR-BPFxLMS is insensitive to step size changes.In terms of tracking performance,its algorithm responds quickly to sudden changes in the noise spectrum and can cope with the complex control requirements of sudden changes in the noise spectrum.
基金supported by Hebei Province Key Research and Development Program(19277770D)Natural Science Foundation of Hebei Province(H2018423026)+2 种基金the Foundation of Health and Family Planning Commission of Hebei(2018068620180688)Fund of Hebei Administration of Traditional Chinese Medicine(2023020).
文摘Collective cancer cell migration(CCCM)and epithelial-to-mesenchymal transition(EMT)play key roles in metastasis.This study reports that the colorectal carcinoma cell line LIM1863 is useful for the study of CCCM and EMT.Methods:Hematoxylin and eosin staining,scanning electron microscopy,transmission electron microscopy,and western blot analysis were performed.Results:LIM1863 automatically grew as spheroids in suspension and had important typical epithelial properties,including several layers of cells arranged around a central lumen,apical-basal polarity,and types of cell-cell junctions.Treatment with a combination of both TGF beta 1 and TNF alpha induced definite and distinct EMT,a spheroid changing phenotype to form a monolayer high-confluent patch without lumen,without polarity.Spontaneous CCCM occurred in spheroids.Flat EMT cells adhered to the base of a dish,exhibited persistent movement as a cluster of cells,and then shed,resulting in a cluster.All cells from one cluster undergoing CCCM died.Otherwise,all cells undergoing EMT disappeared and almost all cells located in the cell reservoir survived and proliferated.Conclusion:LIM1863 is an excellent cell line to study CCCM and EMT.The group of heterogeneous cells undergoing CCCM behaves like a supracellular unit.
文摘Against the existential and ubiquitous threat of climate changes to the environment,human health,and economic well-being,the proliferation of carbon neutrality from both government and private sector recently prevails in order to strengthen preparedness for the future global crisis.More than 70 countries and regions have pledged to achieve the goal of carbon neutrality by 2050(ICAP,2020).While the momentum toward adopting carbon neutrality targets continue to build,a growing body of countries and subnational governments move toward carbon pricing through rolling out,speeding-up,or strengthening the construct of emission trading scheme(ETS)and carbon financial market.Notwithstanding the laudable objectives of ETS to enable emission reduction,the potential of ETS is still largely untapped,largely stemming from poor financial innovation.This special issue is,therefore,aimed at promoting research on the carbon financial innovations,financial derivatives services,the use of Fintech and the optimization on architectures of the ETS such that ETSs could drive the transformation toward low-carbon development path and the achievement of Carbon Neutrality targets.
