The logistics transportation and distribution of fruits and vegetables has become one of the important links for people to obtain food,and it is also an important direction and emerging challenge in the logistics indu...The logistics transportation and distribution of fruits and vegetables has become one of the important links for people to obtain food,and it is also an important direction and emerging challenge in the logistics industry.As the social economy and transportation develop,the consumption ability of residents has been improved,and the high demand for fruits and vegetables has promoted the transportation of fruits and vegetables to meet the development conditions of the future fruit and vegetable industry.The study of fruit and vegetable logistics distribution can improve the efficiency of fruit and vegetable distribution,improve the construction of fruit and vegetable distribution system,and also meet the needs of people for different kinds of fruits and vegetables.Taking Guangxi fruit and vegetable distribution as an example,through empirical investigation,this paper studies the existing problems in the development of logistics distribution in the fruit and vegetable distribution industry,and puts forward corresponding measures and countermeasures according to the problems,so as to innovate the fruit and vegetable distribution mode in Guangxi Zhuang Autonomous Region.展开更多
Demyelination and remyelination have been major focal points in the study of peripheral nerve regeneration following peripheral nerve injury.Notably,the gene regulatory network of regenerated myelin differs from that ...Demyelination and remyelination have been major focal points in the study of peripheral nerve regeneration following peripheral nerve injury.Notably,the gene regulatory network of regenerated myelin differs from that of native myelin.Silencing of enhancer of zeste homolog 2(EZH2)hinders the differentiation,maturation,and myelination of Schwann cells in vitro.To further determine the role of EZH2 in myelination and recovery post-peripheral nerve injury,conditional knockout mice lacking Ezh2 in Schwann cells(Ezh2^(fl/fl);Dhh-Cre and Ezh2^(fl/fl);Mpz-Cre)were generated.Our results show that a significant proportion of axons in the sciatic nerve of Ezh2-depleted mice remain unmyelinated.This highlights the crucial role of Ezh2 in initiating Schwann cell myelination.Furthermore,we observed that 21 days after inducing a sciatic nerve crush injury in these mice,most axons had remyelinated at the injury site in the control nerve,while Ezh2^(fl/fl);Mpz-Cre mice had significantly fewer remyelinated axons compared with their wild-type littermates.This suggests that the absence of Ezh2 in Schwann cells impairs myelin formation and remyelination.In conclusion,EZH2 has emerged as a pivotal regulatory factor in the process of demyelination and myelin regeneration following peripheral nerve injury.Modulating EZH2 activity during these processes may offer a promising therapeutic target for the treatment of peripheral nerve injuries.展开更多
In this work,we reported a series of monolithic 3D-printed Ni-Mo alloy electrodes for highly efficient water splitting at high current density(1500 mA cm^(-2))with excellent stability,which provides a solution to scal...In this work,we reported a series of monolithic 3D-printed Ni-Mo alloy electrodes for highly efficient water splitting at high current density(1500 mA cm^(-2))with excellent stability,which provides a solution to scale up Ni-Mo catalysts for HER to industry use.All possible Ni-Mo metal/alloy phases were achieved by tuning the atomic composition and heat treatment procedure,and they were investigated through both experiment and simulation,and the optimal NiMo phase shows the best performance.Density functional theory(DFT)calculations elucidate that the NiMo phase has the lowest H2O dissociation energy,which further explains the exceptional performance of NiMo.In addition,the microporosity was modulated via controlled thermal treatment,indicating that the 1100℃sintered sample has the best catalytic performance,which is attributed to the high electrochemically active surface area(ECSA).Finally,the four different macrostructures were achieved by 3D printing,and they further improved the catalytic performance.The gyroid structure exhibits the best catalytic performance of driving 500 mA cm^(-2)at a low overpotential of 228 mV and 1500 mA cm^(-2)at 325 mV,as it maximizes the efficient bubble removal from the electrode surface,which offers the great potential for high current density water splitting.展开更多
In this study,we successfully developed a low RE containing Mg-3Y-2Gd-1Nd-0.5Zr(wt%)alloy with high strength-ductility synergy by combined processes of hot extrusion,hot rolling and ageing.This alloy exhibits an excel...In this study,we successfully developed a low RE containing Mg-3Y-2Gd-1Nd-0.5Zr(wt%)alloy with high strength-ductility synergy by combined processes of hot extrusion,hot rolling and ageing.This alloy exhibits an excellent strength-ductility balance(UTS of 345±2.0 MPa,TYS of 301±5.0 MPa and EL of 9.2±1.9%),which is better than that of many Mg-RE wrought alloys with higher RE concentration and even comparable to that of 6061 Al wrought alloy.A long-range chain-like structure consisting ofβphase,βH phase,βM phase and zig-zag atomic columns is observed for the first time in the studied alloy.The combined process of hot extrusion and hot rolling boosts the formation of deformed grains and low angle grain boundaries,and makes the deformed grains dominate in the alloy strengthening.Under this circumstance,the following ageing generates a novel heterogeneous structure comprising the long-range chain-like structure with broad interparticle spacing and the spacious precipitate-free zones in the deformed grains,which plays a key role in the concurrent strengthening and toughening of the alloy.The present study demonstrates that the deformed grains with long-range chain-like structures and precipitate-free zones is desirable microstructure for the low RE containing Mg alloys to achieve high strength-ductility synergy.展开更多
<strong>Objectives:</strong> To evaluate the diagnostic performance of the quantitative iodine parameters, including Iodine Concentration (IC), Normalized Iodine Concentration (NIC), and λ<sub>HU<...<strong>Objectives:</strong> To evaluate the diagnostic performance of the quantitative iodine parameters, including Iodine Concentration (IC), Normalized Iodine Concentration (NIC), and λ<sub>HU</sub>, in distinguishing malignant and benign thyroid nodules. <strong>Methods:</strong> Relevant studies were searched from Web of Science, PubMed, Embase, Cochrane Library, China National Knowledge Infrastructure database and other complementary sources from inception to May 20, 2020. Study selection, data extraction, quality assessment, and data analyses were performed following the Cochrane standards and the PRISMA-DTA guideline. <strong>Results: </strong>Eight studies were included (595 patients with 737 thyroid nodules). The pooled sensitivity, specificity and summary diagnostic odds ratio of IC were 79% (95% CI: 69% - 86%), 76% (95% CI: 65% - 84%) and 11 (95% CI: 5 - 27) respectively;those of NIC were 78% (95% CI: 70% - 85%), 80% (95% CI: 74% - 85%) and 15 (95% CI: 9 - 24) respectively;those of λ<sub>HU</sub> were 80% (95% CI: 71% - 87%), 77% (95% CI: 70% - 83%) and 14 (95% CI: 8 - 24) respectively. <strong>Conclusion: </strong>DECT can be a potential evaluation tool for thyroid nodules. The NIC may be the most sensitive iodine parameter and could be comparable between different DECT machines in thyroid nodule assessment.展开更多
TaiChi pile function originated from the ancient health guidance method in China.Practice has proved that it plays a good role in removing diseases and fitness,prolonging life.Fascia is an anatomical structure formed ...TaiChi pile function originated from the ancient health guidance method in China.Practice has proved that it plays a good role in removing diseases and fitness,prolonging life.Fascia is an anatomical structure formed by connective tissue,one of the four tissues.It is an independent sensory organ and a tension network system running through the whole body[1].展开更多
Claudin 14 has been shown to promote nerve repair and regeneration in the early stages of Wallerian degeneration （0-4 days） in rats with sciatic nerve injury, but the mechanism underlying this process remains poorly...Claudin 14 has been shown to promote nerve repair and regeneration in the early stages of Wallerian degeneration （0-4 days） in rats with sciatic nerve injury, but the mechanism underlying this process remains poorly understood. This study reported the effects of claudin 14 on nerve degeneration and regeneration during early Wallerian degeneration. Claudin 14 expression was up-regulated in sciatic nerve 4 days after Wallerian degeneration. The altered expression of claudin 14 in Schwann cells resulted in expression changes of cytokines in vitro. Expression of claudin 14 affected c-Jun, but not Akt anal ERK1/2 patl^ways, l^urther studies reve^ed that enhanced expression of claudin 14 could promote Schwann cell proliferation and migration. Silencing of claudin 14 expression resulted in Schwann cell apoptosis and reduction in Schwann cell proliferation. Our data revealed the role of claudin 14 in early Wallerian degeneration, which may provide new insights into the molecular mechanisms of Wallerian degeneration.展开更多
Encouraging results have been reported for the use of transcranial magnetic stimulationbased nerve stimulation in studies of the mechanisms of neurological regulation,nerve injury repair,and nerve localization.However...Encouraging results have been reported for the use of transcranial magnetic stimulationbased nerve stimulation in studies of the mechanisms of neurological regulation,nerve injury repair,and nerve localization.However,to date,there are only a few reviews on the use of transcranial magnetic stimulation for diabetic neuropathy.Patients with diabetic neuropathy vary in disease progression and show neuropathy in the early stage of the disease with mild symptoms,making it difficult to screen and identify.In the later stage of the disease,irreversible neurological damage occurs,resulting in treatment difficulties.In this review,we summarize the current state of diabetic neuropathy research and the prospects for the application of transcranial magnetic stimulation in diabetic neuropathy.We review significant studies on the beneficial effects of transcranial magnetic stimulation in diabetic neuropathy treatment,based on the outcomes of its use to treat neurodegeneration,pain,blood flow change,autonomic nervous disorders,vascular endothelial injury,and depression.Collectively,the studies suggest that transcranial magnetic stimulation can produce excitatory/inhibitory stimulation of the cerebral cortex or local areas,promote the remodeling of the nervous system,and that it has good application prospects for the localization of the injury,neuroprotection,and the promotion of nerve regeneration.Therefore,transcranial magnetic stimulation is useful for the screening and early treatment of diabetic neuropathy.transcranial magnetic stimulation can also alleviate pain symptoms by changing the cortical threshold and inhibiting the conduction of sensory information in the thalamo-spinal pathway,and therefore it has therapeutic potential for the treatment of pain and pain-related depressive symptoms in patients with diabetic neuropathy.Additionally,based on the effect of transcranial magnetic stimulation on local blood flow and its ability to change heart rate and urine protein content,transcranial magnetic stimulation has potential in the treatment of autonomic nerve dysfunction and vascular injury in diabetic neuropathy.Furthermore,oxidative stress and the inflammatory response are involved in the process of diabetic neuropathy,and transcranial magnetic stimulation can reduce oxidative damage.The pathological mechanisms of diabetic neuropathy should be further studied in combination with transcranial magnetic stimulation technology.展开更多
The increasing energy requirements to power the modern world has driven active research into more advanced electrochemical energy storage devices(EESD)with both high energy densities and power densities.Wide range of ...The increasing energy requirements to power the modern world has driven active research into more advanced electrochemical energy storage devices(EESD)with both high energy densities and power densities.Wide range of newly discovered materials with promising electrochemical properties has shown great potential for next-generation devices,but their performance is normally associated with contradicting demands of thin electrodes and high mass loading that can be hardly achieved for practical applications.Design of three-dimensional(3D)porous electrodes can increase the mass loading while maintaining the effective charge transport even with thick electrodes,which has proven to be efficient to overcome the limitations.3D structures have also been demonstrated excellent structural stability to withstand strong strains and stresses generated during charge/discharge cycle.3D printing,which can fabricate various delicate and complex structural designs,thus offering brand-new opportunities for the rational design and facile construction of next-generation EESDs.The recent developments in 3D printing of next-generation EESDs with high performance are reviewed.Advanced/multiscale electrode structures,such as hierarchically porous structure that can be constructed via high-resolution 3D printing or with post-treatment,are further emphasized.The ability of current 3D printing techniques to fulfill multimaterial printing to fulfill simple packaging will be covered.展开更多
Wallerian degeneration is a complex biological process that occurs after nerve injury,and involves nerve degeneration and regeneration.Schwann cells play a crucial role in the cellular and molecular events of Walleria...Wallerian degeneration is a complex biological process that occurs after nerve injury,and involves nerve degeneration and regeneration.Schwann cells play a crucial role in the cellular and molecular events of Wallerian degeneration of the peripheral nervous system.However,Wallerian degeneration regulating nerve injury and repair remains largely unknown,especially the early response.We have previously reported some key regulators of Wallerian degeneration after sciatic nerve injury.Baculoviral inhibitor of apoptosis protein repeat-containing protein 3(BIRC3)is an important factor that regulates apoptosis-inhibiting protein.In this study,we established rat models of right sciatic nerve injury.In vitro Schwann cell models were also established and subjected to gene transfection to inhibit and overexpress BIRC3.The data indicated that BIRC3 expression was significantly up-regulated after sciatic nerve injury.Both BIRC3 upregulation and downregulation affected the migration,proliferation and apoptosis of Schwan cells and affected the expression of related factors through activating c-fos and ERK signal pathway.Inhibition of BIRC3 delayed early Wallerian degeneration through inhibiting the apoptosis of Schwann cells after sciatic nerve injury.These findings suggest that BIRC3 plays an important role in peripheral nerve injury repair and regeneration.The study was approved by the Institutional Animal Care and Use Committee of Nantong University,China(approval No.2019-nsfc004)on March 1,2019.展开更多
The morphology and properties of HDPE blends with Zn-SEPDM and GR were studied through SEM and mechanical property test. The results show that as Zn-SEPDM/GR content amounts to 20%, the blend becomes an IPN in structu...The morphology and properties of HDPE blends with Zn-SEPDM and GR were studied through SEM and mechanical property test. The results show that as Zn-SEPDM/GR content amounts to 20%, the blend becomes an IPN in structure, and that a rather high impact and tensile strength of HDPE may be obtained after blending. The antistatic effect, the softening point,and HDT of the blend are higher as compared to HDPE/Zn-SEPDM/ZnSt (zinc stearate).The effect of Zn-SEPDM on the compatibility the morphology and properties of IPP blends were studied by DSC, TEM and mechanical properties test. The results show that as Zn-SEPDM content exceeds 20%. Zn-SEPDM in the blend becomes continuous and an abrupt change in impact strength is incurred there from. Owing to the incorporation of ionic groups into EPDM.the strong interactions betWeen the chains make both the impact and the tensile strength of IPP remarkably higher展开更多
Large cutaneous wounds pose a severe medical problem and may be deadly in cases when regeneration is impaired.Recently,topical stem cell therapy has been realized as a promising strategy for wound healing and skin reg...Large cutaneous wounds pose a severe medical problem and may be deadly in cases when regeneration is impaired.Recently,topical stem cell therapy has been realized as a promising strategy for wound healing and skin regeneration.However,stem cells must be administrated uniformly to the wound area,otherwise treatment will be ineffective,which has been a limitation of current administration methods.Specifically,the delivery pressure and nozzle features of most clinical cell spray devices are unknown,which may significantly affect the viability of sprayed cells and their capacity for proliferation.Herein,we developed a novel pneumatically assisted atomization device(PAAD)in which cell suspensions were uniformly atomized at a low delivery pressure.We optimized the applied fluidic pressure and air pressure to maximize cell survival and function for the application of multiple cell types,while ensuring uniform dispersal across the wound site.Moreover,we found that the application of sprayed umbilical cord-derived mesenchymal stem cells to wound sites significantly accelerated wound healing and promoted appendage regeneration on an excisional cutaneous wound model.Overall,the novel PAAD system delivered living cells uniformly and maintained the viability and differentiation of sprayed cells,strongly suggesting its potential for application in clinical cell therapy,especially for large,irregular,and severe skin wounds.展开更多
Soil moisture is a limiting factor of ecosystem development in the semi-arid Loess Plateau. Characterizing the soil moisture response to its dominant controlling factors, such as land use and topography, and quantifyi...Soil moisture is a limiting factor of ecosystem development in the semi-arid Loess Plateau. Characterizing the soil moisture response to its dominant controlling factors, such as land use and topography, and quantifying the soil-water carrying capacity for revegetation is of great significance for vegetation restoration in this region. In this study, soil moisture was monitored to a depth of 2 m in three land use types(native grassland, introduced grassland,and forestland), two landforms(hillslope and gully),and two slope aspects(sunny and shady) in the Nanxiaohegou watershed of the Loess Plateau,Northwest China. The MIKE SHE model was then applied to simulate the soil moisture dynamics under different conditions and determine the optimal plant coverage. Results showed that the average soil moisture was higher in native grassland than in introduced grassland and Platycladus orientalis forestland for a given topographic condition;however,a high soil moisture content was found in Robinia pseudoacacia forestland, with a value that was even higher than the native grassland of a sunny slope. The divergent results in the two forestlands were likely attributed to the differences in plant coverage. Gully regions and shady slopes usually had higher soil moisture, while lower soil moisture was usually distributed on the hillslope and sunny slope.Furthermore, the mean absolute relative error and Nash-Sutcliffe efficiency coefficient of the MIKE SHE model ranged between 2.8%–7.8% and 0.550–0.902,respectively, indicating that the model could effectively simulate the soil moisture dynamics. The optimal plant coverage was thus determined for hillslope P. orientalis by the model, corresponding to a leaf area index(LAI) value of 1.92. Therefore, for sustainable revegetation on the Loess Plateau,selecting suitable land use types(natural vegetation),controlling the planting density/LAI, and selecting proper planting locations(gully and shady slope regions) should be considered by local policy makers to avoid the over-consumption of soil water resources.展开更多
文摘The logistics transportation and distribution of fruits and vegetables has become one of the important links for people to obtain food,and it is also an important direction and emerging challenge in the logistics industry.As the social economy and transportation develop,the consumption ability of residents has been improved,and the high demand for fruits and vegetables has promoted the transportation of fruits and vegetables to meet the development conditions of the future fruit and vegetable industry.The study of fruit and vegetable logistics distribution can improve the efficiency of fruit and vegetable distribution,improve the construction of fruit and vegetable distribution system,and also meet the needs of people for different kinds of fruits and vegetables.Taking Guangxi fruit and vegetable distribution as an example,through empirical investigation,this paper studies the existing problems in the development of logistics distribution in the fruit and vegetable distribution industry,and puts forward corresponding measures and countermeasures according to the problems,so as to innovate the fruit and vegetable distribution mode in Guangxi Zhuang Autonomous Region.
基金financially supported by the National Natural Science Foundation of China,Nos.82172104(to CX),81873767(to HZ)a grant from Jiangsu Provincial Research Hospital,Nos.YJXYY202204(to HZ),YJXYY202204-ZD04(to HZ)+5 种基金a grant from Jiangsu Provincial Key Medical CenterJiangsu Provincial Medical Innovation Center,No.CXZX202212Jiangsu Provincial Medical Key Discipline,No.ZDXK202240the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)Technology Project of Nantong,No.MS22022008(to HZ)Postgraduate Research&Practice Innovation Program of Jiangsu Province,No.SJCX21_1457(to WW)。
文摘Demyelination and remyelination have been major focal points in the study of peripheral nerve regeneration following peripheral nerve injury.Notably,the gene regulatory network of regenerated myelin differs from that of native myelin.Silencing of enhancer of zeste homolog 2(EZH2)hinders the differentiation,maturation,and myelination of Schwann cells in vitro.To further determine the role of EZH2 in myelination and recovery post-peripheral nerve injury,conditional knockout mice lacking Ezh2 in Schwann cells(Ezh2^(fl/fl);Dhh-Cre and Ezh2^(fl/fl);Mpz-Cre)were generated.Our results show that a significant proportion of axons in the sciatic nerve of Ezh2-depleted mice remain unmyelinated.This highlights the crucial role of Ezh2 in initiating Schwann cell myelination.Furthermore,we observed that 21 days after inducing a sciatic nerve crush injury in these mice,most axons had remyelinated at the injury site in the control nerve,while Ezh2^(fl/fl);Mpz-Cre mice had significantly fewer remyelinated axons compared with their wild-type littermates.This suggests that the absence of Ezh2 in Schwann cells impairs myelin formation and remyelination.In conclusion,EZH2 has emerged as a pivotal regulatory factor in the process of demyelination and myelin regeneration following peripheral nerve injury.Modulating EZH2 activity during these processes may offer a promising therapeutic target for the treatment of peripheral nerve injuries.
文摘In this work,we reported a series of monolithic 3D-printed Ni-Mo alloy electrodes for highly efficient water splitting at high current density(1500 mA cm^(-2))with excellent stability,which provides a solution to scale up Ni-Mo catalysts for HER to industry use.All possible Ni-Mo metal/alloy phases were achieved by tuning the atomic composition and heat treatment procedure,and they were investigated through both experiment and simulation,and the optimal NiMo phase shows the best performance.Density functional theory(DFT)calculations elucidate that the NiMo phase has the lowest H2O dissociation energy,which further explains the exceptional performance of NiMo.In addition,the microporosity was modulated via controlled thermal treatment,indicating that the 1100℃sintered sample has the best catalytic performance,which is attributed to the high electrochemically active surface area(ECSA).Finally,the four different macrostructures were achieved by 3D printing,and they further improved the catalytic performance.The gyroid structure exhibits the best catalytic performance of driving 500 mA cm^(-2)at a low overpotential of 228 mV and 1500 mA cm^(-2)at 325 mV,as it maximizes the efficient bubble removal from the electrode surface,which offers the great potential for high current density water splitting.
基金This work is supported by Beijing Municipal Natural Science Foundation(2202004)National Natural Science Foundation of China(51801048)Basic Research Fund for Newly Enrolled Teachers.
文摘In this study,we successfully developed a low RE containing Mg-3Y-2Gd-1Nd-0.5Zr(wt%)alloy with high strength-ductility synergy by combined processes of hot extrusion,hot rolling and ageing.This alloy exhibits an excellent strength-ductility balance(UTS of 345±2.0 MPa,TYS of 301±5.0 MPa and EL of 9.2±1.9%),which is better than that of many Mg-RE wrought alloys with higher RE concentration and even comparable to that of 6061 Al wrought alloy.A long-range chain-like structure consisting ofβphase,βH phase,βM phase and zig-zag atomic columns is observed for the first time in the studied alloy.The combined process of hot extrusion and hot rolling boosts the formation of deformed grains and low angle grain boundaries,and makes the deformed grains dominate in the alloy strengthening.Under this circumstance,the following ageing generates a novel heterogeneous structure comprising the long-range chain-like structure with broad interparticle spacing and the spacious precipitate-free zones in the deformed grains,which plays a key role in the concurrent strengthening and toughening of the alloy.The present study demonstrates that the deformed grains with long-range chain-like structures and precipitate-free zones is desirable microstructure for the low RE containing Mg alloys to achieve high strength-ductility synergy.
文摘<strong>Objectives:</strong> To evaluate the diagnostic performance of the quantitative iodine parameters, including Iodine Concentration (IC), Normalized Iodine Concentration (NIC), and λ<sub>HU</sub>, in distinguishing malignant and benign thyroid nodules. <strong>Methods:</strong> Relevant studies were searched from Web of Science, PubMed, Embase, Cochrane Library, China National Knowledge Infrastructure database and other complementary sources from inception to May 20, 2020. Study selection, data extraction, quality assessment, and data analyses were performed following the Cochrane standards and the PRISMA-DTA guideline. <strong>Results: </strong>Eight studies were included (595 patients with 737 thyroid nodules). The pooled sensitivity, specificity and summary diagnostic odds ratio of IC were 79% (95% CI: 69% - 86%), 76% (95% CI: 65% - 84%) and 11 (95% CI: 5 - 27) respectively;those of NIC were 78% (95% CI: 70% - 85%), 80% (95% CI: 74% - 85%) and 15 (95% CI: 9 - 24) respectively;those of λ<sub>HU</sub> were 80% (95% CI: 71% - 87%), 77% (95% CI: 70% - 83%) and 14 (95% CI: 8 - 24) respectively. <strong>Conclusion: </strong>DECT can be a potential evaluation tool for thyroid nodules. The NIC may be the most sensitive iodine parameter and could be comparable between different DECT machines in thyroid nodule assessment.
文摘TaiChi pile function originated from the ancient health guidance method in China.Practice has proved that it plays a good role in removing diseases and fitness,prolonging life.Fascia is an anatomical structure formed by connective tissue,one of the four tissues.It is an independent sensory organ and a tension network system running through the whole body[1].
基金supported by grants from the National Natural Science Foundation of China,Grant No.81370982,31170946Key Program,Grant No.81130080+1 种基金the Scientific Research Foundation for Returned Scholars,Ministry of Education of Chinathe Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Claudin 14 has been shown to promote nerve repair and regeneration in the early stages of Wallerian degeneration （0-4 days） in rats with sciatic nerve injury, but the mechanism underlying this process remains poorly understood. This study reported the effects of claudin 14 on nerve degeneration and regeneration during early Wallerian degeneration. Claudin 14 expression was up-regulated in sciatic nerve 4 days after Wallerian degeneration. The altered expression of claudin 14 in Schwann cells resulted in expression changes of cytokines in vitro. Expression of claudin 14 affected c-Jun, but not Akt anal ERK1/2 patl^ways, l^urther studies reve^ed that enhanced expression of claudin 14 could promote Schwann cell proliferation and migration. Silencing of claudin 14 expression resulted in Schwann cell apoptosis and reduction in Schwann cell proliferation. Our data revealed the role of claudin 14 in early Wallerian degeneration, which may provide new insights into the molecular mechanisms of Wallerian degeneration.
基金This work was financially supported by the Science and Technology Project of Nantong City of China,No.JC2018060(to XX).
文摘Encouraging results have been reported for the use of transcranial magnetic stimulationbased nerve stimulation in studies of the mechanisms of neurological regulation,nerve injury repair,and nerve localization.However,to date,there are only a few reviews on the use of transcranial magnetic stimulation for diabetic neuropathy.Patients with diabetic neuropathy vary in disease progression and show neuropathy in the early stage of the disease with mild symptoms,making it difficult to screen and identify.In the later stage of the disease,irreversible neurological damage occurs,resulting in treatment difficulties.In this review,we summarize the current state of diabetic neuropathy research and the prospects for the application of transcranial magnetic stimulation in diabetic neuropathy.We review significant studies on the beneficial effects of transcranial magnetic stimulation in diabetic neuropathy treatment,based on the outcomes of its use to treat neurodegeneration,pain,blood flow change,autonomic nervous disorders,vascular endothelial injury,and depression.Collectively,the studies suggest that transcranial magnetic stimulation can produce excitatory/inhibitory stimulation of the cerebral cortex or local areas,promote the remodeling of the nervous system,and that it has good application prospects for the localization of the injury,neuroprotection,and the promotion of nerve regeneration.Therefore,transcranial magnetic stimulation is useful for the screening and early treatment of diabetic neuropathy.transcranial magnetic stimulation can also alleviate pain symptoms by changing the cortical threshold and inhibiting the conduction of sensory information in the thalamo-spinal pathway,and therefore it has therapeutic potential for the treatment of pain and pain-related depressive symptoms in patients with diabetic neuropathy.Additionally,based on the effect of transcranial magnetic stimulation on local blood flow and its ability to change heart rate and urine protein content,transcranial magnetic stimulation has potential in the treatment of autonomic nerve dysfunction and vascular injury in diabetic neuropathy.Furthermore,oxidative stress and the inflammatory response are involved in the process of diabetic neuropathy,and transcranial magnetic stimulation can reduce oxidative damage.The pathological mechanisms of diabetic neuropathy should be further studied in combination with transcranial magnetic stimulation technology.
基金supports by National Natural Science Foundation of China(grant no.51902265)Fundamental Research Funds for the Central Universities,Key Research and Development Program of Shaanxi(no.2020KWZ-001)Project for graduate Innovation team of Northwestern Polytechnical University.
文摘The increasing energy requirements to power the modern world has driven active research into more advanced electrochemical energy storage devices(EESD)with both high energy densities and power densities.Wide range of newly discovered materials with promising electrochemical properties has shown great potential for next-generation devices,but their performance is normally associated with contradicting demands of thin electrodes and high mass loading that can be hardly achieved for practical applications.Design of three-dimensional(3D)porous electrodes can increase the mass loading while maintaining the effective charge transport even with thick electrodes,which has proven to be efficient to overcome the limitations.3D structures have also been demonstrated excellent structural stability to withstand strong strains and stresses generated during charge/discharge cycle.3D printing,which can fabricate various delicate and complex structural designs,thus offering brand-new opportunities for the rational design and facile construction of next-generation EESDs.The recent developments in 3D printing of next-generation EESDs with high performance are reviewed.Advanced/multiscale electrode structures,such as hierarchically porous structure that can be constructed via high-resolution 3D printing or with post-treatment,are further emphasized.The ability of current 3D printing techniques to fulfill multimaterial printing to fulfill simple packaging will be covered.
基金supported by the National Natural Science Foundation of China,Nos.31971277,31950410551Scientific Research Foundation for Returned Scholars+2 种基金Ministry of Education of ChinaPriority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the Postgraduate Research&Practice Innovation Program of Jiangsu Province of China,No.KYCX 19-2050(all to DBY)。
文摘Wallerian degeneration is a complex biological process that occurs after nerve injury,and involves nerve degeneration and regeneration.Schwann cells play a crucial role in the cellular and molecular events of Wallerian degeneration of the peripheral nervous system.However,Wallerian degeneration regulating nerve injury and repair remains largely unknown,especially the early response.We have previously reported some key regulators of Wallerian degeneration after sciatic nerve injury.Baculoviral inhibitor of apoptosis protein repeat-containing protein 3(BIRC3)is an important factor that regulates apoptosis-inhibiting protein.In this study,we established rat models of right sciatic nerve injury.In vitro Schwann cell models were also established and subjected to gene transfection to inhibit and overexpress BIRC3.The data indicated that BIRC3 expression was significantly up-regulated after sciatic nerve injury.Both BIRC3 upregulation and downregulation affected the migration,proliferation and apoptosis of Schwan cells and affected the expression of related factors through activating c-fos and ERK signal pathway.Inhibition of BIRC3 delayed early Wallerian degeneration through inhibiting the apoptosis of Schwann cells after sciatic nerve injury.These findings suggest that BIRC3 plays an important role in peripheral nerve injury repair and regeneration.The study was approved by the Institutional Animal Care and Use Committee of Nantong University,China(approval No.2019-nsfc004)on March 1,2019.
文摘The morphology and properties of HDPE blends with Zn-SEPDM and GR were studied through SEM and mechanical property test. The results show that as Zn-SEPDM/GR content amounts to 20%, the blend becomes an IPN in structure, and that a rather high impact and tensile strength of HDPE may be obtained after blending. The antistatic effect, the softening point,and HDT of the blend are higher as compared to HDPE/Zn-SEPDM/ZnSt (zinc stearate).The effect of Zn-SEPDM on the compatibility the morphology and properties of IPP blends were studied by DSC, TEM and mechanical properties test. The results show that as Zn-SEPDM content exceeds 20%. Zn-SEPDM in the blend becomes continuous and an abrupt change in impact strength is incurred there from. Owing to the incorporation of ionic groups into EPDM.the strong interactions betWeen the chains make both the impact and the tensile strength of IPP remarkably higher
基金the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA16020807)the Key Research and Development Program of Jiangsu Province,China(Nos.BE2018668 and BE2017669)+2 种基金the Major Innovative Research Team of Suzhou,China(No.ZXT2019007)SIBET and Jilin City Science and Technology Cooperation Project(No.E0550104)Doctor of Entrepreneurship and Innovation Program of Jiangsu Province in the year of 2020.
文摘Large cutaneous wounds pose a severe medical problem and may be deadly in cases when regeneration is impaired.Recently,topical stem cell therapy has been realized as a promising strategy for wound healing and skin regeneration.However,stem cells must be administrated uniformly to the wound area,otherwise treatment will be ineffective,which has been a limitation of current administration methods.Specifically,the delivery pressure and nozzle features of most clinical cell spray devices are unknown,which may significantly affect the viability of sprayed cells and their capacity for proliferation.Herein,we developed a novel pneumatically assisted atomization device(PAAD)in which cell suspensions were uniformly atomized at a low delivery pressure.We optimized the applied fluidic pressure and air pressure to maximize cell survival and function for the application of multiple cell types,while ensuring uniform dispersal across the wound site.Moreover,we found that the application of sprayed umbilical cord-derived mesenchymal stem cells to wound sites significantly accelerated wound healing and promoted appendage regeneration on an excisional cutaneous wound model.Overall,the novel PAAD system delivered living cells uniformly and maintained the viability and differentiation of sprayed cells,strongly suggesting its potential for application in clinical cell therapy,especially for large,irregular,and severe skin wounds.
基金supported financially by the National Natural Science Foundation of China (41771259)the Shanxi Province Science Foundation for Youths (201901D211352)+1 种基金the Shanxi Incentive Foundation for Distinguished Doctorates (SXYBKY2019043)the Innovation Foundation of Science and Technology of Shanxi Agricultural University (2020BQ25)。
文摘Soil moisture is a limiting factor of ecosystem development in the semi-arid Loess Plateau. Characterizing the soil moisture response to its dominant controlling factors, such as land use and topography, and quantifying the soil-water carrying capacity for revegetation is of great significance for vegetation restoration in this region. In this study, soil moisture was monitored to a depth of 2 m in three land use types(native grassland, introduced grassland,and forestland), two landforms(hillslope and gully),and two slope aspects(sunny and shady) in the Nanxiaohegou watershed of the Loess Plateau,Northwest China. The MIKE SHE model was then applied to simulate the soil moisture dynamics under different conditions and determine the optimal plant coverage. Results showed that the average soil moisture was higher in native grassland than in introduced grassland and Platycladus orientalis forestland for a given topographic condition;however,a high soil moisture content was found in Robinia pseudoacacia forestland, with a value that was even higher than the native grassland of a sunny slope. The divergent results in the two forestlands were likely attributed to the differences in plant coverage. Gully regions and shady slopes usually had higher soil moisture, while lower soil moisture was usually distributed on the hillslope and sunny slope.Furthermore, the mean absolute relative error and Nash-Sutcliffe efficiency coefficient of the MIKE SHE model ranged between 2.8%–7.8% and 0.550–0.902,respectively, indicating that the model could effectively simulate the soil moisture dynamics. The optimal plant coverage was thus determined for hillslope P. orientalis by the model, corresponding to a leaf area index(LAI) value of 1.92. Therefore, for sustainable revegetation on the Loess Plateau,selecting suitable land use types(natural vegetation),controlling the planting density/LAI, and selecting proper planting locations(gully and shady slope regions) should be considered by local policy makers to avoid the over-consumption of soil water resources.
