MicroRNA(miR)-200b-3p has been associated with many tumors,but its involvement in pituitary adenoma is unclear.This study investigated the molecular mechanism underlying miR-200b-3p regulation in pituitary adenomas to...MicroRNA(miR)-200b-3p has been associated with many tumors,but its involvement in pituitary adenoma is unclear.This study investigated the molecular mechanism underlying miR-200b-3p regulation in pituitary adenomas to provide a theoretical basis for treatment.Bioinformatics was used to analyze pituitary adenoma-related genes and screen new targets related to RECK and miRNA.As well,the relationship between miR-200b-3p and RECK protein was verified using a double-luciferase reporter gene assay.The expression of miR-200b-3p in clinical samples was analyzed by in situ hybridization.Transfection of the miR-200b-3p inhibitor and small interfering-RECK(si-RECK)was verified by qPCR.GH3 cell viability and proliferation were detected using CCK8 and EdU assays.Apoptosis was detected by flow cytometry and western blotting.Wound healing and Transwell assays were used to detect cell migration and invasion.The effects of miR-200b-3p and RECK on GH3 cells were verified using salvage experiments.miR-200b-3p was highly expressed in pituitary tumor tissue.Inhibitors of miR-200b-3p inhibited cell proliferation promoted cell apoptosis,inhibited invasion and migration,and inhibited the expression of matrix metalloproteinases.Interestingly,miR-200b-3p negatively regulated RECK.The expression of RECK in pituitary adenoma tissues was lower than that in neighboring tissues.Si-RECK rescued the function of miR-200b-3p inhibitors in the above cellular behaviors,and miR-200b-3p accelerated the development of pituitary adenoma by negatively regulating RECK expression.In summary,this study investigated the molecular mechanism by which miR-200b-3p regulates the progression of pituitary adenoma through the negative regulation of RECK.The findings provide a new target for the treatment of pituitary adenoma.展开更多
The development of a selective catalyst for the conversion of biomass and plastics into H2by steam reforming can combat the energy crisis and global warming.In this work,support Ni-Fe-Ca/H-Al bifunctional catalysts we...The development of a selective catalyst for the conversion of biomass and plastics into H2by steam reforming can combat the energy crisis and global warming.In this work,support Ni-Fe-Ca/H-Al bifunctional catalysts were prepared by loading Ni and Fe into pretreatment CaO/Al_(2)O_(3)(Ca/H-Al)carriers and showed high catalytic activity for the steam reforming of biomass and plastic.Moreover,the idea of bidirectional degradation was exploited to strengthen the pyrolysis of plastic with a high H/C and biomass with a high O/C.Interestingly,the products presented high H2selective(1302.10 m L/g)and low CO_(2)yield(120.23 m L/g)in 7Ni-5Fe-Ca/H-Al(2:4)catalyst compared with current reports.Here,the abundant oxygen vacancies(Ov)in the H-Al carrier exhibited an electron-deficient nature,providing active sites for anchoring Ni O.Meanwhile,Ni O interacted with Ca_(2)Fe_(2)O_(5)to produce more defective Ovsites,which stabilized the NiO particles in the 7Ni-5Fe-Ca/H-Al(2:4)catalyst,and the interaction between the catalyst and the carrier was enhanced,leading to the reduction of weakly basic sites,this property promoted the strong adsorption of CO_(2)and H2O by the catalyst,contributing to the enhancement of efficient steam conversion and the promotion of conversion of by-products to H2.Notably,7Ni-5Fe-Ca/H-Al(2:4)catalysts maintained structural integrity after regeneration and exhibited excellent regenerability in H2selection and CO_(2)adsorption.The work provides a new idea for the study of efficient H2production from steam reforming of biomass and plastics.展开更多
Based on the ABAQUS/Explicit code,A 3D elastic-plastic and coupled thermo-mechanical FE model of radial ring rolling of AZ31 Magnesium alloy has been proposed to analyze the influence of rotational speed of driver rol...Based on the ABAQUS/Explicit code,A 3D elastic-plastic and coupled thermo-mechanical FE model of radial ring rolling of AZ31 Magnesium alloy has been proposed to analyze the influence of rotational speed of driver roll to study the inhomogeneity distribution of strain and temperature,fishtail coefficient,rolling force parameters.The results show that:(1)when the rotational speed of driver roll n increases,the strain distribution of the rolled ring becomes less homogeneous,and the temperature distribution more homogeneous yet,and leading to an optimal n value;(2)the fishtail coefficient firstly decreases,then increases with the increase of n;(3)the rolling force,contact area and rolling moment gradually descend with the increase of n.展开更多
Shear behavior of granular soil with fines is investigated using the discrete element method （DEM） and particle arrangements and inter-particle contacts during shear are examined. The DEM simulation reveals that fin...Shear behavior of granular soil with fines is investigated using the discrete element method （DEM） and particle arrangements and inter-particle contacts during shear are examined. The DEM simulation reveals that fine particles play a vital role in the overall response of granular soil to shearing. The occurrence of liquefaction and temporary reduction of strength is ascribed mainly to the loss of support from the fine particle contacts （S-S） and fine particle-to-large particle contacts （S-L） as a consequence of the removal of fine particles from the load-carrying skeleton. The dilative strain-hardening response following the strain-softening response is associated with the migration of fine particles back into the load-carrying skeleton, which is thought to enhance the stiffness of the soil skeleton. During shear, the unit normal vector of the large particle-to-large particle （L-L） contact has the strongest fabric anisotropy, and the S-S contact unit normal vector possesses the weakest anisotropy, suggesting that the large particles play a dominant role in carrying the shear load. It is also found that, during shear, fine particles are prone to rolling at contacts while the large particles are prone to sliding, mainly at the S-L and L-L contacts.展开更多
Unlike healthy, non-transformed cells, the proteostasis network of cancer cells is taxed to produce proteins involved in tumor development. Cancer cells have a higher dependency on molecular chaperones to maintain pro...Unlike healthy, non-transformed cells, the proteostasis network of cancer cells is taxed to produce proteins involved in tumor development. Cancer cells have a higher dependency on molecular chaperones to maintain proteostasis. The chaperonin T-complex protein ring complex(TRiC) contains eight paralogous subunits(CCT1-8), and assists the folding of as many as 10% of cytosolic proteome.TRiC is essential for the progression of some cancers, but the roles of TRiC subunits in osteosarcoma remain to be explored. Here, we show that CCT4/TRiC is significantly correlated in human osteosarcoma,and plays a critical role in osteosarcoma cell survival. We identify a compound anticarin-β that can specifically bind to and inhibit CCT4. Anticarin-β shows higher selectivity in cancer cells than in normal cells. Mechanistically, anticarin-β potently impedes CCT4-mediated STAT3 maturation. Anticarin-β displays remarkable antitumor efficacy in orthotopic and patient-derived xenograft models of osteosarcoma.Collectively, our data uncover a key role of CCT4 in osteosarcoma, and propose a promising treatment strategy for osteosarcoma by disrupting CCT4 and proteostasis.展开更多
Grammar learning has been a bottleneck problem for a long time. In this paper, we propose a method of seman- tic separator learning, a special case of grammar learning. The method is based on the hypothesis that some ...Grammar learning has been a bottleneck problem for a long time. In this paper, we propose a method of seman- tic separator learning, a special case of grammar learning. The method is based on the hypothesis that some classes of words, called semantic separators, split a sentence into sev- eral constituents. The semantic separators are represented by words together with their part-of-speech tags and other infor- mation so that rich semantic information can be involved. In the method, we first identify the semantic separators with the help of noun phrase boundaries, called subseparators. Next, the argument classes of the separators are learned from cor- pus by generalizing argument instances in a hypernym space. Finally, in order to evaluate the learned semantic separators, we use them in unsupervised Chinese text parsing. The exper- iments on a manually labeled test set show that the proposed method outperforms previous methods of unsupervised text parsing.展开更多
MicroRNA (miRNA) is a type of endogenous non-coding RNA that can regulate cell proliferation,differentiation,invasion,apoptosis and several other biological activities by specially inducing gene silencing,and thereby ...MicroRNA (miRNA) is a type of endogenous non-coding RNA that can regulate cell proliferation,differentiation,invasion,apoptosis and several other biological activities by specially inducing gene silencing,and thereby is related to development and disease in life course.In recent years,researchers have found that miRNAs are closely related with refractory epilepsy.MiRNAs can intervene in the modification of mRNAs,the synthesis of proteins and some the connectivity of signal pathways in pathogenesis of epilepsy.Furthermore,some miRNAs in neurons are of great importance in neuronal differentiation.Therefore,miRNA may play a very important role in the occurrence,development and episodes of refractory epilepsy.These discoveries can provide a new direction for the research of pathogenesis,diagnostic methods and therapeutic approach of refractory epilepsy.Although research about miRNA and intractable epilepsy has progressed,more remains to be done before miRNA can be used in clinical diagnosis and treatment strategies.This paper focuses on the research progress of molecular diagnosis about miRNA in intractable epilepsy.展开更多
基金supported by Correlation between RECK and GH-type pituitary adenomas(No.21JR11RE027).
文摘MicroRNA(miR)-200b-3p has been associated with many tumors,but its involvement in pituitary adenoma is unclear.This study investigated the molecular mechanism underlying miR-200b-3p regulation in pituitary adenomas to provide a theoretical basis for treatment.Bioinformatics was used to analyze pituitary adenoma-related genes and screen new targets related to RECK and miRNA.As well,the relationship between miR-200b-3p and RECK protein was verified using a double-luciferase reporter gene assay.The expression of miR-200b-3p in clinical samples was analyzed by in situ hybridization.Transfection of the miR-200b-3p inhibitor and small interfering-RECK(si-RECK)was verified by qPCR.GH3 cell viability and proliferation were detected using CCK8 and EdU assays.Apoptosis was detected by flow cytometry and western blotting.Wound healing and Transwell assays were used to detect cell migration and invasion.The effects of miR-200b-3p and RECK on GH3 cells were verified using salvage experiments.miR-200b-3p was highly expressed in pituitary tumor tissue.Inhibitors of miR-200b-3p inhibited cell proliferation promoted cell apoptosis,inhibited invasion and migration,and inhibited the expression of matrix metalloproteinases.Interestingly,miR-200b-3p negatively regulated RECK.The expression of RECK in pituitary adenoma tissues was lower than that in neighboring tissues.Si-RECK rescued the function of miR-200b-3p inhibitors in the above cellular behaviors,and miR-200b-3p accelerated the development of pituitary adenoma by negatively regulating RECK expression.In summary,this study investigated the molecular mechanism by which miR-200b-3p regulates the progression of pituitary adenoma through the negative regulation of RECK.The findings provide a new target for the treatment of pituitary adenoma.
基金the National Natural Science of China(21968037)the Reserve Program for Young and Middle-aged Academic and Technical Leaders in Yunnan Province(202205AC160031)+1 种基金the Research Innovation Project of Yunnan University for Graduate Students on Exemption,the Highlevel Talent Promotion and Training Project of Kunming(2022SCP003)advanced analysis and measurement center of Yunnan university for the sample testing service。
文摘The development of a selective catalyst for the conversion of biomass and plastics into H2by steam reforming can combat the energy crisis and global warming.In this work,support Ni-Fe-Ca/H-Al bifunctional catalysts were prepared by loading Ni and Fe into pretreatment CaO/Al_(2)O_(3)(Ca/H-Al)carriers and showed high catalytic activity for the steam reforming of biomass and plastic.Moreover,the idea of bidirectional degradation was exploited to strengthen the pyrolysis of plastic with a high H/C and biomass with a high O/C.Interestingly,the products presented high H2selective(1302.10 m L/g)and low CO_(2)yield(120.23 m L/g)in 7Ni-5Fe-Ca/H-Al(2:4)catalyst compared with current reports.Here,the abundant oxygen vacancies(Ov)in the H-Al carrier exhibited an electron-deficient nature,providing active sites for anchoring Ni O.Meanwhile,Ni O interacted with Ca_(2)Fe_(2)O_(5)to produce more defective Ovsites,which stabilized the NiO particles in the 7Ni-5Fe-Ca/H-Al(2:4)catalyst,and the interaction between the catalyst and the carrier was enhanced,leading to the reduction of weakly basic sites,this property promoted the strong adsorption of CO_(2)and H2O by the catalyst,contributing to the enhancement of efficient steam conversion and the promotion of conversion of by-products to H2.Notably,7Ni-5Fe-Ca/H-Al(2:4)catalysts maintained structural integrity after regeneration and exhibited excellent regenerability in H2selection and CO_(2)adsorption.The work provides a new idea for the study of efficient H2production from steam reforming of biomass and plastics.
基金The authors are grateful for the financial support of Project Supported by:Chongqing Municipal Engineering Research Center of Institutions of Higher Education(cstc2011ptgc70007)Project Supported by Program for Innovation Team Building at Institutions of Higher Education in Chongqing(201028)Achievement Transfer Program of Institutions of Higher Education in Chongqing(kjzh11221).
文摘Based on the ABAQUS/Explicit code,A 3D elastic-plastic and coupled thermo-mechanical FE model of radial ring rolling of AZ31 Magnesium alloy has been proposed to analyze the influence of rotational speed of driver roll to study the inhomogeneity distribution of strain and temperature,fishtail coefficient,rolling force parameters.The results show that:(1)when the rotational speed of driver roll n increases,the strain distribution of the rolled ring becomes less homogeneous,and the temperature distribution more homogeneous yet,and leading to an optimal n value;(2)the fishtail coefficient firstly decreases,then increases with the increase of n;(3)the rolling force,contact area and rolling moment gradually descend with the increase of n.
基金the University of Hong Kong for its financial support under the Seed Funding for Basic Research Scheme(20121159028)the National Natural Science Foundation of China(No.51209237)the Fundamental Research Funds for the Central Universities(No.131gpy05)
文摘Shear behavior of granular soil with fines is investigated using the discrete element method （DEM） and particle arrangements and inter-particle contacts during shear are examined. The DEM simulation reveals that fine particles play a vital role in the overall response of granular soil to shearing. The occurrence of liquefaction and temporary reduction of strength is ascribed mainly to the loss of support from the fine particle contacts （S-S） and fine particle-to-large particle contacts （S-L） as a consequence of the removal of fine particles from the load-carrying skeleton. The dilative strain-hardening response following the strain-softening response is associated with the migration of fine particles back into the load-carrying skeleton, which is thought to enhance the stiffness of the soil skeleton. During shear, the unit normal vector of the large particle-to-large particle （L-L） contact has the strongest fabric anisotropy, and the S-S contact unit normal vector possesses the weakest anisotropy, suggesting that the large particles play a dominant role in carrying the shear load. It is also found that, during shear, fine particles are prone to rolling at contacts while the large particles are prone to sliding, mainly at the S-L and L-L contacts.
基金the National Natural Science Foundation of China(81903666 and 31930015)the Chinese Academy of Sciences(XDB31000000,KFJ-STS-SCYD-304,and K.C.Wong Education Foundation,China)+4 种基金the Science and Technology Department of Yunnan Province (202101AT070301,2019ZF003,202002AA100007,202003AD150008,and 2019FB103China)Project of Innovative Research Team of Yunnan Province(2019HC005China)the Department of Industry and Information Technology of Yunnan Province (2019-YT-053,China)。
文摘Unlike healthy, non-transformed cells, the proteostasis network of cancer cells is taxed to produce proteins involved in tumor development. Cancer cells have a higher dependency on molecular chaperones to maintain proteostasis. The chaperonin T-complex protein ring complex(TRiC) contains eight paralogous subunits(CCT1-8), and assists the folding of as many as 10% of cytosolic proteome.TRiC is essential for the progression of some cancers, but the roles of TRiC subunits in osteosarcoma remain to be explored. Here, we show that CCT4/TRiC is significantly correlated in human osteosarcoma,and plays a critical role in osteosarcoma cell survival. We identify a compound anticarin-β that can specifically bind to and inhibit CCT4. Anticarin-β shows higher selectivity in cancer cells than in normal cells. Mechanistically, anticarin-β potently impedes CCT4-mediated STAT3 maturation. Anticarin-β displays remarkable antitumor efficacy in orthotopic and patient-derived xenograft models of osteosarcoma.Collectively, our data uncover a key role of CCT4 in osteosarcoma, and propose a promising treatment strategy for osteosarcoma by disrupting CCT4 and proteostasis.
文摘Grammar learning has been a bottleneck problem for a long time. In this paper, we propose a method of seman- tic separator learning, a special case of grammar learning. The method is based on the hypothesis that some classes of words, called semantic separators, split a sentence into sev- eral constituents. The semantic separators are represented by words together with their part-of-speech tags and other infor- mation so that rich semantic information can be involved. In the method, we first identify the semantic separators with the help of noun phrase boundaries, called subseparators. Next, the argument classes of the separators are learned from cor- pus by generalizing argument instances in a hypernym space. Finally, in order to evaluate the learned semantic separators, we use them in unsupervised Chinese text parsing. The exper- iments on a manually labeled test set show that the proposed method outperforms previous methods of unsupervised text parsing.
文摘MicroRNA (miRNA) is a type of endogenous non-coding RNA that can regulate cell proliferation,differentiation,invasion,apoptosis and several other biological activities by specially inducing gene silencing,and thereby is related to development and disease in life course.In recent years,researchers have found that miRNAs are closely related with refractory epilepsy.MiRNAs can intervene in the modification of mRNAs,the synthesis of proteins and some the connectivity of signal pathways in pathogenesis of epilepsy.Furthermore,some miRNAs in neurons are of great importance in neuronal differentiation.Therefore,miRNA may play a very important role in the occurrence,development and episodes of refractory epilepsy.These discoveries can provide a new direction for the research of pathogenesis,diagnostic methods and therapeutic approach of refractory epilepsy.Although research about miRNA and intractable epilepsy has progressed,more remains to be done before miRNA can be used in clinical diagnosis and treatment strategies.This paper focuses on the research progress of molecular diagnosis about miRNA in intractable epilepsy.
