Chimeric antigen receptor T-cesll therapy(CAR–T)has achieved groundbreaking advancements in clinical application,ushering in a new era for innovative cancer treatment.However,the challenges associated with implementi...Chimeric antigen receptor T-cesll therapy(CAR–T)has achieved groundbreaking advancements in clinical application,ushering in a new era for innovative cancer treatment.However,the challenges associated with implementing this novel targeted cell therapy are increasingly significant.Particularly in the clinical management of solid tumors,obstacles such as the immunosuppressive effects of the tumor microenvironment,limited local tumor infiltration capability of CAR–T cells,heterogeneity of tumor targeting antigens,uncertainties surrounding CAR–T quality,control,and clinical adverse reactions have contributed to increased drug resistance and decreased compliance in tumor therapy.These factors have significantly impeded the widespread adoption and utilization of this therapeutic approach.In this paper,we comprehensively analyze recent preclinical and clinical reports on CAR–T therapy while summarizing crucial factors influencing its efficacy.Furthermore,we aim to identify existing solution strategies and explore their current research status.Through this review article,our objective is to broaden perspectives for further exploration into CAR–T therapy strategies and their clinical applications.展开更多
To measure miss distance for antiaircraft projectile,a radial velocity identification and positioning method with a single radar is proposed. By analyzing the spatial resolution of multi-frequency ranging radar,the di...To measure miss distance for antiaircraft projectile,a radial velocity identification and positioning method with a single radar is proposed. By analyzing the spatial resolution of multi-frequency ranging radar,the discrimination and testing model of this radar for multi-targets（ projectile and target） is established to analyze the systematic error of antiaircraft miss distance. Then through the aerial target flight test and contrast test with optical test equipment,the validity of the measurement method is verified. This newmethod has the potential to be used in the measurement of antiaircraft projectile miss distance.展开更多
Previous studies have demonstrated that nerve cells differentiated from adipose-derived stro-mal cells after chemical induction have reduced viability;however, the underlying mechanisms remained unclear. In this study...Previous studies have demonstrated that nerve cells differentiated from adipose-derived stro-mal cells after chemical induction have reduced viability;however, the underlying mechanisms remained unclear. In this study, we induced the differentiation of adult adipose-derived stromal cells into astrocytes using chemical induction. 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetra-zolium bromide assay and flow cytometry showed that, with increasing induction time, the apoptotic rate gradually increased, and the number of living cells gradually decreased. Im-munohistochemical staining demonstrated that the number of glial fibrillary acidic protein-, caspase-3- and caspase-9-positive cells gradually increased with increasing induction time. Transmission electron microscopy revealed typical signs of apoptosis after differentiation. Taken together, our results indicate that caspase-dependent apoptosis is an obstacle to the differentia-tion of adipose-derived stromal cells into astrocytes. Inhibiting apoptosis may be an important strategy for increasing the efifciency of induction.展开更多
BACKGROUND： The detection of motor evoked potential is utilized to explore neuromuscular finger coordination. The influence of transcranial magnetic stimulation on finger force has been investigated mainly on a singl...BACKGROUND： The detection of motor evoked potential is utilized to explore neuromuscular finger coordination. The influence of transcranial magnetic stimulation on finger force has been investigated mainly on a single finger, and only time-dependent increased target finger force has been detected in the finger force task. OBJECTIVE： To explore the neural mechanism of finger force coordination in the motor cortex by observing the influence of various finger coordination patterns and patterns of transcranial magnetic stimulation （TMS）-induced finger force changes. DESIGN, TIME AND SETTING： Neurophysiological and behavioral study was performed at the Biomedical Engineering Laboratory of Chongqing University from April to June 2008. PARTICIPANTS： A total of 10 healthy, university students, comprising 5 males and 5 females, aged 21-23 years, voluntarily participated in this study. All participants were right-handed, with normal or corrected vision. Individuals with upper limb complaints or other musculoskeletal disorders were excluded. METHODS： A target force-tracking task was conducted on the index finger, the index and middle fingers, and four fingers （index, middle, ring, and little）, respectively. Target force trace in a single trial consisted of a 6-second ramp phase, a 20-second constant phase, and a 6-second drop phase. During experimentation, an unpredictable single-pulse TMS （120% motor threshold） was applied to the primary motor cortex （M1） in each phase. MAIN OUTCOME MEASURES： Changes in peak force induced by TMS were obtained for each finger pattern during each force-tracking phase. Differences in force changes were tested between different finger pattems with regard to ramp, constant, and drop phases of target force. RESULTS： Under ramp, constant, and drop phases of target force, the increase in magnetic stimulation-induced finger forces changes positively correlated with the number of fingers involved in the force tracking task. The magnetic stimulation-induced force changes from the index finger were less than the combination of the index and middle fingers or all four fingers under the corresponding target force, and the force changes from the combination of the index and middle fingers were less than all four fingers, Le., index finger 〈 index and middle fingers 〈 four fingers. CONCLUSION： Different neuromuscular mechanisms could be involved in finger force production for different finger combination patterns. Results from the present study suggested that independent motor neurons regulated individual finger force production.展开更多
Bone marrow mesenchymal stem cell transplantation is an effective treatment for neonatal hy- poxic-ischemic brain damage. However, the in vivo transplantation effects are poor and their survival, colonization and diff...Bone marrow mesenchymal stem cell transplantation is an effective treatment for neonatal hy- poxic-ischemic brain damage. However, the in vivo transplantation effects are poor and their survival, colonization and differentiation efficiencies are relatively low. Red or near-infrared light from 600-1,000 nm promotes cellular migration and prevents apoptosis. Thus, we hypothesized that the combination of red light with bone marrow mesenchymal stem cell transplantation would be effective for the treatment of hypoxic-ischemic brain damage. In this study, the migra- tion and colonization of cultured bone marrow mesenchymal stem cells on primary neurons after oxygen-glucose deprivation were detected using Transwell assay. The results showed that, after a 40-hour irradiation under red light-emitting diodes at 660 nm and 60 mW/cmz, an increasing number of green fluorescence-labeled bone marrow mesenchymal stem cells migrated towards hypoxic-ischemic damaged primary neurons. Meanwhile, neonatal rats with hypoxic-ischemic brain damage were given an intraperitoneal injection of 1 x 106 bone marrow mesenchymal stem cells, followed by irradiation under red light-emitting diodes at 660 nm and 60 mW/cm2 for 7 successive days. Shuttle box test results showed that, after phototherapy and bone marrow mesenchymal stem cell transplantation, the active avoidance response rate of hypoxic-ischemic brain damage rats was significantly increased, which was higher than that after bone marrow mesenchymal stem cell transplantation alone. Experimental findings indicate that 660 nm red light emitting diode irradiation promotes cells, thereby enhancing the contribution ic-ischemic brain damage. the migration of bone marrow mesenchymal stem of cell transplantation in the treatment of hypox-展开更多
A better understanding of the neural mechanisms of finger-force regulation can help to explain the relationship between the central nervous system and nerve-muscle force, as well as assist in motor functional rehabili...A better understanding of the neural mechanisms of finger-force regulation can help to explain the relationship between the central nervous system and nerve-muscle force, as well as assist in motor functional rehabilitation and the development robot hand designs. In the present study, 11 healthy volunteers performed a different target force-tracking task, which involved the index finger alone, index and middle finger together, and the combination of four fingers （i.e., index, middle, ring, and little）. The target force trace corresponded to 3 levels of 20% maximal voluntary changes （MVC）, 30% MVC, and 40% MVC in 20 seconds. In the test, an unexpected single 120% motor threshold transcranial magnetic stimulation was applied to the primary motor cortex （M1） during force tracking. Results revealed that peak force changes increased with increasing background force and the number of involved task fingers. These results demonstrate that M1 neural activities correlate with finger-force production, and M1 plays a role in finger-force control. Moreover, different neuronal networks were required for different finger patterns; a complicated task required multi-finger combinations and a complicated neuronal network comprised a large number of neurons.展开更多
The problem of measuring exterior ballistic feature points is always difficult to solve and it is essentiale on exterior ballistic measurement.By analysis of radar reflection characteristics and non-stationary echo si...The problem of measuring exterior ballistic feature points is always difficult to solve and it is essentiale on exterior ballistic measurement.By analysis of radar reflection characteristics and non-stationary echo signals of exterior ballistic feature points,the echo data of exterior ballistic feature points is measured by using the continuous wave radar.The parameters of feature points are extracted by the empirical mode decomposition method(EMD)of Hilbert-Huang transform(HHT)spectrum analysis technique.The radar echo signal model and EMD extraction model are established to analyze the exterior ballistic mutation point detection and EMD extraction method of aliasing echo signal.Typical feature point parameters of exterior ballistic in rocket flight tests are carried out and the effectiveness of the method is verified.A new method of measuring the parameters of exterior ballistic feature point is therefore presented.展开更多
Renal amyloidosis secondary to anti-neutrophil cytoplasmic antibody(ANCA)-associated vasculitis is extremely rare.Here,we reported a 77-year-old woman with ANCA-associated vasculitis.Renal biopsy with Masson trichrome...Renal amyloidosis secondary to anti-neutrophil cytoplasmic antibody(ANCA)-associated vasculitis is extremely rare.Here,we reported a 77-year-old woman with ANCA-associated vasculitis.Renal biopsy with Masson trichrome staining showed pauci-immune crescentic glomerulonephritis,and electron microscopy showed amyloid deposition in the mesangial area.Immunofluorescence revealed kappa light chain and lambda light chain negative.Bone marrow biopsy revealed no clonal plasma cell.Finally,she was diagnosed as ANCA-associated vasculitis with secondary renal amyloid A amyloidosis.展开更多
[Objectives]To investigate the inhibitory effect and possible mechanism of essential oil from Valerianae Jatamansi Rhizoma et Radix on microglia activation induced by lipopolysaccharide(LPS).[Methods]The LPS-induced m...[Objectives]To investigate the inhibitory effect and possible mechanism of essential oil from Valerianae Jatamansi Rhizoma et Radix on microglia activation induced by lipopolysaccharide(LPS).[Methods]The LPS-induced microglia activation model was established and treated with different doses of essential oil from Valerianae Jatamansi Rhizoma et Radix.MTT assay was used to detect cell viability,ELISA to detect IL-6 secretion,RT-PCR to detect mRNA expression levels of IL-6,IL-1β,NF-κB,and IκBα,Western blotting to detect the protein expression of IL-6,IL-1β,NF-κB,IκBα,and their phosphorylated products.[Results]Compared with the normal control group,the model group showed increased IL-6 content(P<0.01),upregulated mRNA and protein levels of IL-6,IL-1β,and NF-κB(P<0.01),and elevated ratio of P-IκBα/IκBα(P<0.05).Compared with the model group,4 and 2μg/L essential oil from Valerianae Jatamansi Rhizoma et Radix reduced the content of IL-6(P<0.05),while 4,2,and 1μg/L essential oil from Valerianae Jatamansi Rhizoma et Radix down-regulated the mRNA and protein levels of IL-6,IL-1β,and NF-κB to varying degrees(P<0.05 or P<0.01),up-regulate the mRNA expression of IκBα(P<0.05 or P<0.01),and decreased the ratio of P-IκBα/IκBα(P<0.05 or P<0.01).[Conclusions]Essential oil from Valerianae Jatamansi Rhizoma et Radix can inhibit LPS-induced microglia activation,and its mechanism may be related to the inhibition of the NF-κB/IκBαsignaling pathway.展开更多
What is already known about this topic?S.1,4,[5],12:i:-and S.Rissen are emerging serotypes of Salmonella that require close monitoring for antimicrobial resistance and containment of their spread.What is added by this...What is already known about this topic?S.1,4,[5],12:i:-and S.Rissen are emerging serotypes of Salmonella that require close monitoring for antimicrobial resistance and containment of their spread.What is added by this report?The study aimed to identify antimicrobial resistance genes(ARGs)in S.1,4,[5],12:i:-and S.Rissen strains isolated from environmental sewage in Guangzhou City,Guangdong Province,China.A phylogenetic tree was constructed using single nucleotide polymorphism data to assess genetic relatedness among strains,offering insights for Salmonella infection outbreak investigations in the future.What are the implications for public health practice?It is crucial to implement strategies,such as integrating different networks,to control the spread of drugresistant Salmonella.Novel technologies must be utilized to disinfect sewage and eliminate ARGs.Ensuring food safety and proper sewage disinfection are essential to curb the dissemination of Salmonella.展开更多
We show that through strong ligand mediated interfacial energy control between Au seeds and the deposited Au,the non-wetting growth of Au on Au seeds led to the formation homometallic core-satellite nanostructures.To ...We show that through strong ligand mediated interfacial energy control between Au seeds and the deposited Au,the non-wetting growth of Au on Au seeds led to the formation homometallic core-satellite nanostructures.To modulate the intraparticle plasmonic coupling between the core and the satellites,the number and size of the Au satellites,and their inter-island distances were continuously tuned by varying the growth kinetics.As a result of the precise structural control,the plasmonic absorptions of the core-satellite nanostructures were tuned from visible to near-infrared(NIR)spectral range,and the extent of spectral modulation(500-1300 nm)is among the best of the literature methods.This synthetic advance enriches the toolbox for nanosynthesis and points to a new direction in the exploration of sophisticated functional designs.展开更多
Precise and continuous monitoring of biochemicals by biosensors assists to understand physiological functions for various diagnostics and therapeutic applications.For implanted biosensors,small size and flexibility ar...Precise and continuous monitoring of biochemicals by biosensors assists to understand physiological functions for various diagnostics and therapeutic applications.For implanted biosensors,small size and flexibility are essential for minimizing tissue damage and achieving accurate detection.However,the active surface area of sensor decreases as the sensor becomes smaller,which will increase the impedance and decrease the signal to noise ratio,resulting in a poor detection limit.Taking advantages of local amplification effect,organic electrochemical transistors(OECTs)constitute promising candidates for high-sensitive monitoring.However,their detections in deep tissues are rarely reported.Herein,we report a family of implantable,fiber-shaped all-in-one OECTs based on carbon nanotube fibers for versatile biochemical detection including H2O2,glucose,dopamine and glutamate.These fiber-shaped OECTs demonstrated high sensitivity,dynamical stability in physiological environment and antiinterference capability.After implantation in mouse brain,7-day dopamine monitoring in vivo was realized for the first time.These fiber-shaped OECTs could be great additions to the"life science"tool box and represent promising avenue for biomedical monitoring.展开更多
Due to the mechanical mismatch between conventional rigid electronic devices and soft tissues at nature,a lot of interests have been attracted to develop flexible bioelectronics that work well both in vitro and in viv...Due to the mechanical mismatch between conventional rigid electronic devices and soft tissues at nature,a lot of interests have been attracted to develop flexible bioelectronics that work well both in vitro and in vivo. To this end, polymers that can be used for both key components and substrates are indispensable to achieve high performances such as high sensitivity and long-term stability for sensing applications.Here we will summarize the recent advances on the synthesis of a variety of polymers, the design of typical architectures and the integration of different functions for the flexible bioelectronic devices. The remaining challenges and promising directions are highlighted to provide inspirations for the future study on the emerging flexible bioelectronics at end.展开更多
The UFMylation modification is a novel ubiquitin-like conjugation system,consisting of UBA5(E1),UFC1(E2),UFL1(E3),and the conjugating molecule UFM1.Deficiency in this modification leads to embryonic lethality in mice ...The UFMylation modification is a novel ubiquitin-like conjugation system,consisting of UBA5(E1),UFC1(E2),UFL1(E3),and the conjugating molecule UFM1.Deficiency in this modification leads to embryonic lethality in mice and diseases in humans.However,the function of UFL1 is poorly characterized.Studies on Ufl1 conditional knockout mice have demonstrated that the deletion of Ufl1 in cardiomyocytes and in intestinal epithelial cells causes heart failure and increases susceptibility to experimentally induced colitis,respectively,suggesting an essential role of UFL1 in the maintenance of the homeostasis in these organs.Yet,its physiological function in other tissues and organs remains completely unknown.In this study,we generate the nephron tubules specific Ufl1 knockout mice and find that the absence of Ufl1 in renal tubular results in kidney atrophy and interstitial fibrosis.In addition,Ufl1 deficiency causes the activation of unfolded protein response and cell apoptosis,which may be responsible for the kidney atrophy and interstitial fibrosis.Collectively,our results have demonstrated the crucial role of UFL1 in regulating kidney function and maintenance of endoplasmic reticulum homeostasis,providing another layer of understanding kidney atrophy.展开更多
基金funded by 2023 Sichuan Scientific and Technological Achievements Transformation Project.Project Number:2023JDZH0024.
文摘Chimeric antigen receptor T-cesll therapy(CAR–T)has achieved groundbreaking advancements in clinical application,ushering in a new era for innovative cancer treatment.However,the challenges associated with implementing this novel targeted cell therapy are increasingly significant.Particularly in the clinical management of solid tumors,obstacles such as the immunosuppressive effects of the tumor microenvironment,limited local tumor infiltration capability of CAR–T cells,heterogeneity of tumor targeting antigens,uncertainties surrounding CAR–T quality,control,and clinical adverse reactions have contributed to increased drug resistance and decreased compliance in tumor therapy.These factors have significantly impeded the widespread adoption and utilization of this therapeutic approach.In this paper,we comprehensively analyze recent preclinical and clinical reports on CAR–T therapy while summarizing crucial factors influencing its efficacy.Furthermore,we aim to identify existing solution strategies and explore their current research status.Through this review article,our objective is to broaden perspectives for further exploration into CAR–T therapy strategies and their clinical applications.
基金Supported by the National Natural Science Foundation of China(61174219,51677192)
文摘To measure miss distance for antiaircraft projectile,a radial velocity identification and positioning method with a single radar is proposed. By analyzing the spatial resolution of multi-frequency ranging radar,the discrimination and testing model of this radar for multi-targets（ projectile and target） is established to analyze the systematic error of antiaircraft miss distance. Then through the aerial target flight test and contrast test with optical test equipment,the validity of the measurement method is verified. This newmethod has the potential to be used in the measurement of antiaircraft projectile miss distance.
文摘Previous studies have demonstrated that nerve cells differentiated from adipose-derived stro-mal cells after chemical induction have reduced viability;however, the underlying mechanisms remained unclear. In this study, we induced the differentiation of adult adipose-derived stromal cells into astrocytes using chemical induction. 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetra-zolium bromide assay and flow cytometry showed that, with increasing induction time, the apoptotic rate gradually increased, and the number of living cells gradually decreased. Im-munohistochemical staining demonstrated that the number of glial fibrillary acidic protein-, caspase-3- and caspase-9-positive cells gradually increased with increasing induction time. Transmission electron microscopy revealed typical signs of apoptosis after differentiation. Taken together, our results indicate that caspase-dependent apoptosis is an obstacle to the differentia-tion of adipose-derived stromal cells into astrocytes. Inhibiting apoptosis may be an important strategy for increasing the efifciency of induction.
基金the National Natural Science Foundation of China, No. 3077054630970758+1 种基金Chongqing Natural Science Foundation, No. 2006BB2043 2007BB5148
文摘BACKGROUND： The detection of motor evoked potential is utilized to explore neuromuscular finger coordination. The influence of transcranial magnetic stimulation on finger force has been investigated mainly on a single finger, and only time-dependent increased target finger force has been detected in the finger force task. OBJECTIVE： To explore the neural mechanism of finger force coordination in the motor cortex by observing the influence of various finger coordination patterns and patterns of transcranial magnetic stimulation （TMS）-induced finger force changes. DESIGN, TIME AND SETTING： Neurophysiological and behavioral study was performed at the Biomedical Engineering Laboratory of Chongqing University from April to June 2008. PARTICIPANTS： A total of 10 healthy, university students, comprising 5 males and 5 females, aged 21-23 years, voluntarily participated in this study. All participants were right-handed, with normal or corrected vision. Individuals with upper limb complaints or other musculoskeletal disorders were excluded. METHODS： A target force-tracking task was conducted on the index finger, the index and middle fingers, and four fingers （index, middle, ring, and little）, respectively. Target force trace in a single trial consisted of a 6-second ramp phase, a 20-second constant phase, and a 6-second drop phase. During experimentation, an unpredictable single-pulse TMS （120% motor threshold） was applied to the primary motor cortex （M1） in each phase. MAIN OUTCOME MEASURES： Changes in peak force induced by TMS were obtained for each finger pattern during each force-tracking phase. Differences in force changes were tested between different finger pattems with regard to ramp, constant, and drop phases of target force. RESULTS： Under ramp, constant, and drop phases of target force, the increase in magnetic stimulation-induced finger forces changes positively correlated with the number of fingers involved in the force tracking task. The magnetic stimulation-induced force changes from the index finger were less than the combination of the index and middle fingers or all four fingers under the corresponding target force, and the force changes from the combination of the index and middle fingers were less than all four fingers, Le., index finger 〈 index and middle fingers 〈 four fingers. CONCLUSION： Different neuromuscular mechanisms could be involved in finger force production for different finger combination patterns. Results from the present study suggested that independent motor neurons regulated individual finger force production.
基金the National Natural Science Foundation of China,No.30970758,31271060the National Science and Technology Support Program of China,No.2011BAI14B04,2012BAI16B02the Natural Science Foundation of Chongqing in China,No.cst-c2012jjA10103
文摘Bone marrow mesenchymal stem cell transplantation is an effective treatment for neonatal hy- poxic-ischemic brain damage. However, the in vivo transplantation effects are poor and their survival, colonization and differentiation efficiencies are relatively low. Red or near-infrared light from 600-1,000 nm promotes cellular migration and prevents apoptosis. Thus, we hypothesized that the combination of red light with bone marrow mesenchymal stem cell transplantation would be effective for the treatment of hypoxic-ischemic brain damage. In this study, the migra- tion and colonization of cultured bone marrow mesenchymal stem cells on primary neurons after oxygen-glucose deprivation were detected using Transwell assay. The results showed that, after a 40-hour irradiation under red light-emitting diodes at 660 nm and 60 mW/cmz, an increasing number of green fluorescence-labeled bone marrow mesenchymal stem cells migrated towards hypoxic-ischemic damaged primary neurons. Meanwhile, neonatal rats with hypoxic-ischemic brain damage were given an intraperitoneal injection of 1 x 106 bone marrow mesenchymal stem cells, followed by irradiation under red light-emitting diodes at 660 nm and 60 mW/cm2 for 7 successive days. Shuttle box test results showed that, after phototherapy and bone marrow mesenchymal stem cell transplantation, the active avoidance response rate of hypoxic-ischemic brain damage rats was significantly increased, which was higher than that after bone marrow mesenchymal stem cell transplantation alone. Experimental findings indicate that 660 nm red light emitting diode irradiation promotes cells, thereby enhancing the contribution ic-ischemic brain damage. the migration of bone marrow mesenchymal stem of cell transplantation in the treatment of hypox-
基金the Fundamental Research Funds for the Central Universities,No.CDJZR11230002
文摘A better understanding of the neural mechanisms of finger-force regulation can help to explain the relationship between the central nervous system and nerve-muscle force, as well as assist in motor functional rehabilitation and the development robot hand designs. In the present study, 11 healthy volunteers performed a different target force-tracking task, which involved the index finger alone, index and middle finger together, and the combination of four fingers （i.e., index, middle, ring, and little）. The target force trace corresponded to 3 levels of 20% maximal voluntary changes （MVC）, 30% MVC, and 40% MVC in 20 seconds. In the test, an unexpected single 120% motor threshold transcranial magnetic stimulation was applied to the primary motor cortex （M1） during force tracking. Results revealed that peak force changes increased with increasing background force and the number of involved task fingers. These results demonstrate that M1 neural activities correlate with finger-force production, and M1 plays a role in finger-force control. Moreover, different neuronal networks were required for different finger patterns; a complicated task required multi-finger combinations and a complicated neuronal network comprised a large number of neurons.
基金Supported by the National Natural Science Foundation of China(61174219,51677192)
文摘The problem of measuring exterior ballistic feature points is always difficult to solve and it is essentiale on exterior ballistic measurement.By analysis of radar reflection characteristics and non-stationary echo signals of exterior ballistic feature points,the echo data of exterior ballistic feature points is measured by using the continuous wave radar.The parameters of feature points are extracted by the empirical mode decomposition method(EMD)of Hilbert-Huang transform(HHT)spectrum analysis technique.The radar echo signal model and EMD extraction model are established to analyze the exterior ballistic mutation point detection and EMD extraction method of aliasing echo signal.Typical feature point parameters of exterior ballistic in rocket flight tests are carried out and the effectiveness of the method is verified.A new method of measuring the parameters of exterior ballistic feature point is therefore presented.
文摘Renal amyloidosis secondary to anti-neutrophil cytoplasmic antibody(ANCA)-associated vasculitis is extremely rare.Here,we reported a 77-year-old woman with ANCA-associated vasculitis.Renal biopsy with Masson trichrome staining showed pauci-immune crescentic glomerulonephritis,and electron microscopy showed amyloid deposition in the mesangial area.Immunofluorescence revealed kappa light chain and lambda light chain negative.Bone marrow biopsy revealed no clonal plasma cell.Finally,she was diagnosed as ANCA-associated vasculitis with secondary renal amyloid A amyloidosis.
基金Supported by Karst Center Project of National Natural Science Foundation of China(U1812403-4-4)High-level Innovative Talents Project in Guizhou Province of Guizhou Provincial Department of Science and Technology[QianKeHeRenCai(2015)4029]+2 种基金Science and Technology Innovation Team for Activity Research of Characteristic Natural Medicine Resources in Guizhou Province[QianKeHeRenCaiTuanDui(2015)4025]Major Project of National Social Science Fund(16ZDA238)Pharmaceutical International Science and Technology Cooperation Base of Guizhou Medical University[QianKeHePingTaiRenCai(2017)5802].
文摘[Objectives]To investigate the inhibitory effect and possible mechanism of essential oil from Valerianae Jatamansi Rhizoma et Radix on microglia activation induced by lipopolysaccharide(LPS).[Methods]The LPS-induced microglia activation model was established and treated with different doses of essential oil from Valerianae Jatamansi Rhizoma et Radix.MTT assay was used to detect cell viability,ELISA to detect IL-6 secretion,RT-PCR to detect mRNA expression levels of IL-6,IL-1β,NF-κB,and IκBα,Western blotting to detect the protein expression of IL-6,IL-1β,NF-κB,IκBα,and their phosphorylated products.[Results]Compared with the normal control group,the model group showed increased IL-6 content(P<0.01),upregulated mRNA and protein levels of IL-6,IL-1β,and NF-κB(P<0.01),and elevated ratio of P-IκBα/IκBα(P<0.05).Compared with the model group,4 and 2μg/L essential oil from Valerianae Jatamansi Rhizoma et Radix reduced the content of IL-6(P<0.05),while 4,2,and 1μg/L essential oil from Valerianae Jatamansi Rhizoma et Radix down-regulated the mRNA and protein levels of IL-6,IL-1β,and NF-κB to varying degrees(P<0.05 or P<0.01),up-regulate the mRNA expression of IκBα(P<0.05 or P<0.01),and decreased the ratio of P-IκBα/IκBα(P<0.05 or P<0.01).[Conclusions]Essential oil from Valerianae Jatamansi Rhizoma et Radix can inhibit LPS-induced microglia activation,and its mechanism may be related to the inhibition of the NF-κB/IκBαsignaling pathway.
基金Supported by Guangdong Basic and Applied Basic Research Foundation(2021A1515012539)Science and Technology Program of Guangzhou+8 种基金China(202102080295)Guangzhou Key Medical Discipline(2021-2023-11)2023 Huadu District Medical and Health General Scientific Research Special Project of Guangzhou Huadu District Bureau of ScienceTechnologyIndustryCommerceand Information Technology(23-HDWS-079)Key R&D Plan of Guangzhou Science and Technology Project(202206080003)Guangzhou Science and Technology Plan Project(2023A03J0938).
文摘What is already known about this topic?S.1,4,[5],12:i:-and S.Rissen are emerging serotypes of Salmonella that require close monitoring for antimicrobial resistance and containment of their spread.What is added by this report?The study aimed to identify antimicrobial resistance genes(ARGs)in S.1,4,[5],12:i:-and S.Rissen strains isolated from environmental sewage in Guangzhou City,Guangdong Province,China.A phylogenetic tree was constructed using single nucleotide polymorphism data to assess genetic relatedness among strains,offering insights for Salmonella infection outbreak investigations in the future.What are the implications for public health practice?It is crucial to implement strategies,such as integrating different networks,to control the spread of drugresistant Salmonella.Novel technologies must be utilized to disinfect sewage and eliminate ARGs.Ensuring food safety and proper sewage disinfection are essential to curb the dissemination of Salmonella.
基金We gratefully acknowledge the financial support from the National Natural Science Foundation of China:General Program(No.21673117,HC)Major Program(No.91956109,HC)+2 种基金Zhejiang Provincial Natural Science Foundation of China:Major Program(No.2022XHSJJ002,HC)Jiangsu Science and Technology Plan(No.BK20211258,YF)Start-up Fund from Westlake University。
文摘We show that through strong ligand mediated interfacial energy control between Au seeds and the deposited Au,the non-wetting growth of Au on Au seeds led to the formation homometallic core-satellite nanostructures.To modulate the intraparticle plasmonic coupling between the core and the satellites,the number and size of the Au satellites,and their inter-island distances were continuously tuned by varying the growth kinetics.As a result of the precise structural control,the plasmonic absorptions of the core-satellite nanostructures were tuned from visible to near-infrared(NIR)spectral range,and the extent of spectral modulation(500-1300 nm)is among the best of the literature methods.This synthetic advance enriches the toolbox for nanosynthesis and points to a new direction in the exploration of sophisticated functional designs.
基金the National Natural Science Foundation of China(2163400351673043)+3 种基金Ministry of Science and Technology of China(2016YFA0203302)Science and Technology Commission of Shanghai Municipality(17QA1400400)Shanghai Municipal Education Commission(2017-01-07-00-07-E00062)Yanchang Petroleum Group。
文摘Precise and continuous monitoring of biochemicals by biosensors assists to understand physiological functions for various diagnostics and therapeutic applications.For implanted biosensors,small size and flexibility are essential for minimizing tissue damage and achieving accurate detection.However,the active surface area of sensor decreases as the sensor becomes smaller,which will increase the impedance and decrease the signal to noise ratio,resulting in a poor detection limit.Taking advantages of local amplification effect,organic electrochemical transistors(OECTs)constitute promising candidates for high-sensitive monitoring.However,their detections in deep tissues are rarely reported.Herein,we report a family of implantable,fiber-shaped all-in-one OECTs based on carbon nanotube fibers for versatile biochemical detection including H2O2,glucose,dopamine and glutamate.These fiber-shaped OECTs demonstrated high sensitivity,dynamical stability in physiological environment and antiinterference capability.After implantation in mouse brain,7-day dopamine monitoring in vivo was realized for the first time.These fiber-shaped OECTs could be great additions to the"life science"tool box and represent promising avenue for biomedical monitoring.
基金supported by the National Key R&D Program of China (2016YFA0203302)the National Natural Science Foundation of China (21634003, 51573027, 51403038, 51673043, and 21604012)the Science and Technology Commission of Shanghai Municipality (16JC1400702, 15XD1500400, and 15JC1490200)
文摘Due to the mechanical mismatch between conventional rigid electronic devices and soft tissues at nature,a lot of interests have been attracted to develop flexible bioelectronics that work well both in vitro and in vivo. To this end, polymers that can be used for both key components and substrates are indispensable to achieve high performances such as high sensitivity and long-term stability for sensing applications.Here we will summarize the recent advances on the synthesis of a variety of polymers, the design of typical architectures and the integration of different functions for the flexible bioelectronic devices. The remaining challenges and promising directions are highlighted to provide inspirations for the future study on the emerging flexible bioelectronics at end.
基金supported by grants from the National Natural Science Foundation of China(31871416,31730020)the Natural Science Foundation of ZhejiangProvince of China(LY18C070001)the Hangzhou Science and Technology Bureau(20182014B01,20180533B27)。
文摘The UFMylation modification is a novel ubiquitin-like conjugation system,consisting of UBA5(E1),UFC1(E2),UFL1(E3),and the conjugating molecule UFM1.Deficiency in this modification leads to embryonic lethality in mice and diseases in humans.However,the function of UFL1 is poorly characterized.Studies on Ufl1 conditional knockout mice have demonstrated that the deletion of Ufl1 in cardiomyocytes and in intestinal epithelial cells causes heart failure and increases susceptibility to experimentally induced colitis,respectively,suggesting an essential role of UFL1 in the maintenance of the homeostasis in these organs.Yet,its physiological function in other tissues and organs remains completely unknown.In this study,we generate the nephron tubules specific Ufl1 knockout mice and find that the absence of Ufl1 in renal tubular results in kidney atrophy and interstitial fibrosis.In addition,Ufl1 deficiency causes the activation of unfolded protein response and cell apoptosis,which may be responsible for the kidney atrophy and interstitial fibrosis.Collectively,our results have demonstrated the crucial role of UFL1 in regulating kidney function and maintenance of endoplasmic reticulum homeostasis,providing another layer of understanding kidney atrophy.
