目的研究白血病相关基因对T细胞急性淋巴细胞白血病(T-ALL)耐药性的影响。方法对38例成人初治T细胞急性淋巴细胞白血病患者通过高通量测序技术检测54种白血病相关基因,将突变率最高基因与81种常用化疗药物在不同浓度下的敏感性相对比,...目的研究白血病相关基因对T细胞急性淋巴细胞白血病(T-ALL)耐药性的影响。方法对38例成人初治T细胞急性淋巴细胞白血病患者通过高通量测序技术检测54种白血病相关基因,将突变率最高基因与81种常用化疗药物在不同浓度下的敏感性相对比,探讨该基因与药物敏感性之间的相关性,同时与实际临床疗效相比较,并研究该基因的临床意义。结果在54种白血病相关性基因中,NOTCH1基因突变率最高,38例患者中18例发生NOTCH1突变,突变组对0.37、1.1、3.3 μmol/L地塞米松药物耐药性高于非突变组( P <0.05),突变组血小板水平低于非突变组( P <0.05),但NOTCH1突变与性别、年龄、白细胞计数、骨髓原始细胞数、血红蛋白水平、LDH水平无相关性( P >0.05)。结论 NOTCH1突变可引起地塞米松耐药,可能为预后不佳指标。展开更多
Poly(lactic-co-glycolic acid)(PLGA)is one of the most representative degradable copolymers and promising drug carriers.In the current paper,the PLGAs with a lactic acid/glycolic acid(LA/GA)molar ratio of 52/48 and var...Poly(lactic-co-glycolic acid)(PLGA)is one of the most representative degradable copolymers and promising drug carriers.In the current paper,the PLGAs with a lactic acid/glycolic acid(LA/GA)molar ratio of 52/48 and various molecular weights were prepared by a melting method.The molecular weight,molecular weight distribution,and thermal stability were determined by 1H NMR and thermogravimetric analysis methods.The results demonstrated that PLGAs with the fixed LA/GA molar ratio(52/48),different molecular weights,and narrow molecular weight distribution could be obtained by solely altering the reaction time.The PLGA films were prepared,and their properties including micro-structure,mechanical property,in-vitro cytotoxicity,and biodegradability were characterized.In combination with the homogeneous microstructures and mechanical properties,the drug-loading and releasing properties of PLGA3.2 films were investigated.The results show that PLGA3.2 film with an LA/GA molar ratio of 52/48 is a promising curcumin carrier.展开更多
In this study, experiments have been performed for an investigation on heat transfer of water in an inclined downward tube with an inner diameter of 20 mm and an inclined angle of 45° from the horizon, with the r...In this study, experiments have been performed for an investigation on heat transfer of water in an inclined downward tube with an inner diameter of 20 mm and an inclined angle of 45° from the horizon, with the range of pressure from 11.5 to 28 MPa, mass flux from 450 to 1550 kg/(m2 s), and heat flux from 50 to 585 k W/m2. Based on the experimental data, the temperature distribution in the tube wall was derived. The heat transfer characteristics of inclined downward flow were compared with that of vertical downward flow. The effects of heat flux on wall temperature were analyzed and the corresponding empirical correlations were presented. The results show that heat transfer characteristics of water in the inclined downward tube are not uniform along the circumference from the top surface to the bottom surface. An increase in heat flux exacerbates the non-uniformity. At subcritical pressures, both dry-out and departure from nucleate boiling(DNB) occur at the top surface of the inclined downward tube; inversely, only dry-out takes place on the bottom surface of the inclined downward tube and in the vertical downward tube. At near-critical pressures, DNB and dry-out occur in the comparing tubes with greater possibility. At supercritical pressures, heat transfer gets enhanced in the pseudo-critical enthalpy region; in the high enthalpy region, the top surface temperature of the inclined downward tube decreases obviously.展开更多
In order to expand the study on flow instability of supercritical circulating fluidized bed(CFB) boiler,a new numerical computational model considering the heat storage of the tube wall metal was presented in this pap...In order to expand the study on flow instability of supercritical circulating fluidized bed(CFB) boiler,a new numerical computational model considering the heat storage of the tube wall metal was presented in this paper.The lumped parameter method was proposed for wall temperature calculation and the single channel model was adopted for the analysis of flow instability.Based on the time-domain method,a new numerical computational program suitable for the analysis of flow instability in the water wall of supercritical CFB boiler with annular furnace was established.To verify the code,calculation results were respectively compared with data of commercial software.According to the comparisons,the new code was proved to be reasonable and accurate for practical engineering application in analysis of flow instability.Based on the new program,the flow instability of supercritical CFB boiler with annular furnace was simulated by time-domain method.When 1.2 times heat load disturbance was applied on the loop,results showed that the inlet flow rate,outlet flow rate and wall temperature fluctuated with time eventually remained at constant values,suggesting that the hydrodynamic flow was stable.The results also showed that in the case of considering the heat storage,the flow in the water wall is easier to return to stable state than without considering heat storage.展开更多
文摘目的研究白血病相关基因对T细胞急性淋巴细胞白血病(T-ALL)耐药性的影响。方法对38例成人初治T细胞急性淋巴细胞白血病患者通过高通量测序技术检测54种白血病相关基因,将突变率最高基因与81种常用化疗药物在不同浓度下的敏感性相对比,探讨该基因与药物敏感性之间的相关性,同时与实际临床疗效相比较,并研究该基因的临床意义。结果在54种白血病相关性基因中,NOTCH1基因突变率最高,38例患者中18例发生NOTCH1突变,突变组对0.37、1.1、3.3 μmol/L地塞米松药物耐药性高于非突变组( P <0.05),突变组血小板水平低于非突变组( P <0.05),但NOTCH1突变与性别、年龄、白细胞计数、骨髓原始细胞数、血红蛋白水平、LDH水平无相关性( P >0.05)。结论 NOTCH1突变可引起地塞米松耐药,可能为预后不佳指标。
基金Major Research and Development Project of Shandong Province,China(No.2019JZZY011118)Program for Scientific Research Innovation Team in Colleges and Universities of Shandong Province,China and the Project of the Jinan Science and Technology Bureau,China(No.2019GXRC021).
文摘Poly(lactic-co-glycolic acid)(PLGA)is one of the most representative degradable copolymers and promising drug carriers.In the current paper,the PLGAs with a lactic acid/glycolic acid(LA/GA)molar ratio of 52/48 and various molecular weights were prepared by a melting method.The molecular weight,molecular weight distribution,and thermal stability were determined by 1H NMR and thermogravimetric analysis methods.The results demonstrated that PLGAs with the fixed LA/GA molar ratio(52/48),different molecular weights,and narrow molecular weight distribution could be obtained by solely altering the reaction time.The PLGA films were prepared,and their properties including micro-structure,mechanical property,in-vitro cytotoxicity,and biodegradability were characterized.In combination with the homogeneous microstructures and mechanical properties,the drug-loading and releasing properties of PLGA3.2 films were investigated.The results show that PLGA3.2 film with an LA/GA molar ratio of 52/48 is a promising curcumin carrier.
基金supported by the "Strategic Priority Research Program" Demonstration of Key Technologies for Clean and Efficient Utilization of Low-rank Coal (Grant No. XDA07030100)
文摘In this study, experiments have been performed for an investigation on heat transfer of water in an inclined downward tube with an inner diameter of 20 mm and an inclined angle of 45° from the horizon, with the range of pressure from 11.5 to 28 MPa, mass flux from 450 to 1550 kg/(m2 s), and heat flux from 50 to 585 k W/m2. Based on the experimental data, the temperature distribution in the tube wall was derived. The heat transfer characteristics of inclined downward flow were compared with that of vertical downward flow. The effects of heat flux on wall temperature were analyzed and the corresponding empirical correlations were presented. The results show that heat transfer characteristics of water in the inclined downward tube are not uniform along the circumference from the top surface to the bottom surface. An increase in heat flux exacerbates the non-uniformity. At subcritical pressures, both dry-out and departure from nucleate boiling(DNB) occur at the top surface of the inclined downward tube; inversely, only dry-out takes place on the bottom surface of the inclined downward tube and in the vertical downward tube. At near-critical pressures, DNB and dry-out occur in the comparing tubes with greater possibility. At supercritical pressures, heat transfer gets enhanced in the pseudo-critical enthalpy region; in the high enthalpy region, the top surface temperature of the inclined downward tube decreases obviously.
基金supported by the "Strategic Priority Research Program" of the Chinese Academy of Sciences,Grant No.XDA07030100the National Key Technology R&D Program of China during the 12th Five-Year Plan Period No.2015BAA03B01-01
文摘In order to expand the study on flow instability of supercritical circulating fluidized bed(CFB) boiler,a new numerical computational model considering the heat storage of the tube wall metal was presented in this paper.The lumped parameter method was proposed for wall temperature calculation and the single channel model was adopted for the analysis of flow instability.Based on the time-domain method,a new numerical computational program suitable for the analysis of flow instability in the water wall of supercritical CFB boiler with annular furnace was established.To verify the code,calculation results were respectively compared with data of commercial software.According to the comparisons,the new code was proved to be reasonable and accurate for practical engineering application in analysis of flow instability.Based on the new program,the flow instability of supercritical CFB boiler with annular furnace was simulated by time-domain method.When 1.2 times heat load disturbance was applied on the loop,results showed that the inlet flow rate,outlet flow rate and wall temperature fluctuated with time eventually remained at constant values,suggesting that the hydrodynamic flow was stable.The results also showed that in the case of considering the heat storage,the flow in the water wall is easier to return to stable state than without considering heat storage.
