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A comprehensive evaluation of factors affecting the accuracy of pig genotype imputation using a single or multi-breed reference population 被引量:2
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作者 ZHANG Kai-li PENG Xia +6 位作者 ZHANG Sai-xian ZHAN Hui-wen LU Jia-hui xie sheng-song ZHAO Shu-hong LI Xin-yun MA Yun-long 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2022年第2期486-495,共10页
Genotype imputation has become an indispensable part of genomic data analysis. In recent years, imputation based on a multi-breed reference population has received more attention, but the relevant studies are scarce i... Genotype imputation has become an indispensable part of genomic data analysis. In recent years, imputation based on a multi-breed reference population has received more attention, but the relevant studies are scarce in pigs. In this study, we used the Illumina Porcine SNP50 Bead Chip to investigate the variations of imputation accuracy with various influencing factors and compared the imputation performance of four commonly used imputation software programs. The results indicated that imputation accuracy increased as either the validation population marker density, reference population sample size, or minor allele frequency(MAF) increased. However, the imputation accuracy would have a certain extent of decrease when the pig reference population was a mixed group of multiple breeds or lines. Considering both imputation accuracy and running time, Beagle 4.1 and FImpute are excellent choices among the four software packages tested. This work visually presents the impacts of these influencing factors on imputation and provides a reference for formulating reasonable imputation strategies in actual pig breeding. 展开更多
关键词 genotype imputation multi-breed reference population imputation accuracy
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Production of homeobox A10 gene transgenic pigs by somatic cell nuclear transfer
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作者 XIAO Qian ZHAO Chang-zhi +7 位作者 LIN Rui-yi LI Guang-lei LI Chang-chun WANG Hai-yan XU Jing xie sheng-song YU Mei ZHAO Shu-hong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2019年第5期1072-1079,共8页
Homeobox A10(Hoxa10) gene is one of the most important candidate genes associated with the reproductive performance of humans and mice. Overexpression of Hoxa10 in mouse endometrium can increase litter size. Moreover,... Homeobox A10(Hoxa10) gene is one of the most important candidate genes associated with the reproductive performance of humans and mice. Overexpression of Hoxa10 in mouse endometrium can increase litter size. Moreover, Hoxa10 plays a key role in regulating the embryo implantation of sows. This study aimed to generate transgenic pigs using Hoxa10 via somatic cell nuclear transfer(SCNT). We established seven Hoxa10-transgenic cell lines, and two of the cell lines were selected as nuclear donors for the transfer. A total of 1 270 cloned embryos were generated and transferred to five surrogate mothers(Landrace×Yorkshire). Eight cloned male piglets were produced including one with cryptorchidism. Six transgenic piglets grew up healthy and produced 56 offspring. Finally, we obtained six transgenic male pigs and 26 transgenic positive offspring that can be used to further study the regulatory mechanism of Hoxa10 on the reproductive performance of pigs. 展开更多
关键词 HOXA10 GENE transgenic pig SOMATIC cell nuclear transfer fetal FIBROBLASTS OOCYTE
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