Objective:To evaluate the efficacy of allicin combined with cyclophosphamide on neuroblastoma(NB)-bearing mice and explore the immunological mechanism in it.Methods:A total of 30NB-bearing mice were equally randomized...Objective:To evaluate the efficacy of allicin combined with cyclophosphamide on neuroblastoma(NB)-bearing mice and explore the immunological mechanism in it.Methods:A total of 30NB-bearing mice were equally randomized into model group,cyclophosphamide group and combined therapy group,10 nudemice were set as normal saline(NS) group.Cyclophosphamide group and combined therapy group were weekly injected with 60 mg/kg cyclophosphamide for four weeks;besides,combined therapy group was given with allicin(10 mg/kg/d) by gastric perfusion for 4 weeks;model group and NS group were given with the same volume of NS.Serum VEGF content was detected by ELISA pre-treating(0 d) and on the 3rd d.14 th d and 28 th d;on29th d,all mice were sacrificed and the tumor,liver,spleen and thymic tissues were weighted.Tumors were made into paraffin section for detecting tumor cell apoptosis and proliferation by TUNEI.and BrdU method,respectively.Survival curves were drawn by Kaplan-Meier method.Results:After treatment,both treatment groups relieved on viscera indexes,VEGF level,T cell subsets distribution and tumor growth and each index of combined therapy group was better than cyclophosphamide group(P【0.05 or 0.01);only combined therapy group could significantly increase the lifetime of NB-bearing mice(χ~2=5.667,P=0.017).Conclusions:Allicin can improve T cell subsets distribution and inhibit VEGF expression through its immunomodulatory activity,thereby improve the efficiency on NB in coordination with cyclophosphamide.展开更多
Objective: To investigate the change of serum inflammatory factors, von Willebrand factor (vWf), vascular endothelial growth factor (VEGF) and adhesion molecule levels in diabetic nephropathy patients (DN), and their ...Objective: To investigate the change of serum inflammatory factors, von Willebrand factor (vWf), vascular endothelial growth factor (VEGF) and adhesion molecule levels in diabetic nephropathy patients (DN), and their significance in the detection of diabetic nephropathy. Methods: According to urinary albumin excretion rate (UAER), a total of 158 cases of diabetic patients were divided into T2DM group (Simple diabetes group, n=52) and DN group (diabetic nephropathy group, n=106), group DN were divided into microalbuminuria group (n=54) and macroalbuminuria group (n=52);At the same time, 50 healthy subjects were selected as control group, the levels of serum inflammatory factors (hs-CRP, IL-6 and TNF-α), vWf, VEGF and adhesion molecules (sVCAM-1, sICAM-1 and E- selectin) in these four groups were compared. Results: The levels of hs-CRP, IL-6, vWf, VEGF, TNF-α, sVCAM-1, sICAM-1 and E- selectin in the T2DM group and DN group were significantly higher than those in the control group, and DN group was significantly higher than that in T2DM group, the difference was statistically significant;In the DN group, compared with microalbuminuria group, the levels of hs-CRP, IL-6, vWf, VEGF, TNF-α, sVCAM-1, sICAM-1 and E- selectin in the macroalbuminuria group were significantly increased, the difference was statistically significant. Conclusion: Serum inflammatory factors, vWf, VEGF and adhesion molecules may play important roles in the course of the genesis and development of DN. It has great value to the assessment of this disease.展开更多
基金Supported by the National Natural Science Foundation of China with Grant No.ul204815
文摘Objective:To evaluate the efficacy of allicin combined with cyclophosphamide on neuroblastoma(NB)-bearing mice and explore the immunological mechanism in it.Methods:A total of 30NB-bearing mice were equally randomized into model group,cyclophosphamide group and combined therapy group,10 nudemice were set as normal saline(NS) group.Cyclophosphamide group and combined therapy group were weekly injected with 60 mg/kg cyclophosphamide for four weeks;besides,combined therapy group was given with allicin(10 mg/kg/d) by gastric perfusion for 4 weeks;model group and NS group were given with the same volume of NS.Serum VEGF content was detected by ELISA pre-treating(0 d) and on the 3rd d.14 th d and 28 th d;on29th d,all mice were sacrificed and the tumor,liver,spleen and thymic tissues were weighted.Tumors were made into paraffin section for detecting tumor cell apoptosis and proliferation by TUNEI.and BrdU method,respectively.Survival curves were drawn by Kaplan-Meier method.Results:After treatment,both treatment groups relieved on viscera indexes,VEGF level,T cell subsets distribution and tumor growth and each index of combined therapy group was better than cyclophosphamide group(P【0.05 or 0.01);only combined therapy group could significantly increase the lifetime of NB-bearing mice(χ~2=5.667,P=0.017).Conclusions:Allicin can improve T cell subsets distribution and inhibit VEGF expression through its immunomodulatory activity,thereby improve the efficiency on NB in coordination with cyclophosphamide.
文摘Objective: To investigate the change of serum inflammatory factors, von Willebrand factor (vWf), vascular endothelial growth factor (VEGF) and adhesion molecule levels in diabetic nephropathy patients (DN), and their significance in the detection of diabetic nephropathy. Methods: According to urinary albumin excretion rate (UAER), a total of 158 cases of diabetic patients were divided into T2DM group (Simple diabetes group, n=52) and DN group (diabetic nephropathy group, n=106), group DN were divided into microalbuminuria group (n=54) and macroalbuminuria group (n=52);At the same time, 50 healthy subjects were selected as control group, the levels of serum inflammatory factors (hs-CRP, IL-6 and TNF-α), vWf, VEGF and adhesion molecules (sVCAM-1, sICAM-1 and E- selectin) in these four groups were compared. Results: The levels of hs-CRP, IL-6, vWf, VEGF, TNF-α, sVCAM-1, sICAM-1 and E- selectin in the T2DM group and DN group were significantly higher than those in the control group, and DN group was significantly higher than that in T2DM group, the difference was statistically significant;In the DN group, compared with microalbuminuria group, the levels of hs-CRP, IL-6, vWf, VEGF, TNF-α, sVCAM-1, sICAM-1 and E- selectin in the macroalbuminuria group were significantly increased, the difference was statistically significant. Conclusion: Serum inflammatory factors, vWf, VEGF and adhesion molecules may play important roles in the course of the genesis and development of DN. It has great value to the assessment of this disease.
