Fermentation substrates of rice with different milling degrees(MDs) were prepared and fermented with human feces to compare their fermentation properties and effects on gut microbiota.MD 0s,MD 5s and MD 60s represente...Fermentation substrates of rice with different milling degrees(MDs) were prepared and fermented with human feces to compare their fermentation properties and effects on gut microbiota.MD 0s,MD 5s and MD 60s represented brown rice,moderately-milled rice and white rice,respectively.After in vitro fermentation,the MD 5s group showed higher starch utilization,compared with the MD 0s and 60s groups evaluated by Fourier transform infrared spectrometer,and confocal laser scanning microscope.Effects of fermentation substrates of rice with different MDs on gut microbiota were evaluated by 16S rDNA sequencing.All the sample groups reduced the pH and produced short-chain fatty acids(SCFAs) and branched-chain fatty acids.The MD 5s group exhibited higher α-diversity than the MD 0s and 60s groups.Abundances of Phascolarctobacterium,Blautia and norank_f_Ruminococcaceae were higher in the MD 0s and 5s groups,compared with the MD 60s group.These bacteria were also positively correlated with the SCFAs production via Spearman correlation analysis.In vitro culture assay revealed that fermentation substrates of MD 0s and 5s promoted the growth of two probiotics(Akkermansia muciniphila and Bifidobacterium adolescentis).Our results showed that moderate milling might be an appropriate way to produce rice products with richer nutrients and better fermentation properties.展开更多
Background:Gastric cancer(GC)is a malignancy with the worst prognosis that seriously threatens human health,especially in East Asia.Apolipoprotein C1(apoc1)belongs to the apolipoprotein family.In addition,apoc1 has be...Background:Gastric cancer(GC)is a malignancy with the worst prognosis that seriously threatens human health,especially in East Asia.Apolipoprotein C1(apoc1)belongs to the apolipoprotein family.In addition,apoc1 has been associated with various tumors.However,its role in GC remains unclear.Methods:Firstly,we quantified its expression in GC and adjacent tumor tissues,using The Cancer Genome Atlas(TCGA).Next,we assessed cell invasion and migration abilities.Finally,we revealed the role of apoc1 in the tumor microenvironment(TME),immune cell infiltration and drug sensitivity.Results:Firstly,in TCGA database,it has been shown that elevated expression of apoc1 was identified in various cancers,including GC,then we found that high expression of apoc1 was significantly correlated with poor prognosis in GC.Histologically,apoc1 expression is proportional to grade,cancer stage,and T stage.The experimental results showed that apoc1 promoted cell invasion and migration.Then GO,KEGG,and GSEA pathway analyses indicated that apoc1 may be involved in the WNT pathway and immune regulation.Furthermore,we found out the tumor-infiltrating immune cells related to apoc1 in the tumor microenvironment(TME)using TIMER.Finally,we investigated the correlation between apoc1 expression and drug sensitivity,PD-1 and CTLA-4 therapy.Conclusions:These results suggest that apoc1 participates in the evolution of GC,and may represent a potential target for detection and immunotherapy in GC.展开更多
Background:Clear-cell renal cell carcinoma(ccRCC)is the most common malignant kidney cancer.However,the tumor microenvironment and crosstalk involved in metabolic reprogramming in ccRCC are not well-understood.Methods...Background:Clear-cell renal cell carcinoma(ccRCC)is the most common malignant kidney cancer.However,the tumor microenvironment and crosstalk involved in metabolic reprogramming in ccRCC are not well-understood.Methods:We used The Cancer Genome Atlas to obtain ccRCC transcriptome data and clinical information.The EMTAB-1980 cohort was used for external validation.The GENECARDS database contains the first 100 solute carrier(SLC)-related genes.The predictive value of SLC-related genes for ccRCC prognosis and treatment was assessed using univariate Cox regression analysis.An SLC-related predictive signature was developed through Lasso regression analysis and used to determine the risk profiles of patients with ccRCC.Patients in each cohort were separated into high-and low-risk groups based on their risk scores.The clinical importance of the signature was assessed through survival,immune microenvironment,drug sensitivity,and nomogram analyses using R software.Results:SLC25A23,SLC25A42,SLC5A1,SLC3A1,SLC25A37,SLC5A6,SLCO5A1,and SCP2 comprised the signatures of the eight SLCrelated genes.Patients with ccRCC were separated into high-and low-risk groups based on the risk value in the training and validation cohorts;the high-risk group had a significantly worse prognosis(p<0.001).The risk score was an independent predictive indicator of ccRCC in the two cohorts according to univariate and multivariate Cox regression(p<0.05).Analysis of the immune microenvironment showed that immune cell infiltration and immune checkpoint gene expression differed between the two groups(p<0.05).Drug sensitivity analysis showed that compared to the low-risk group,the high-risk group was more sensitive to sunitinib,nilotinib,JNK-inhibitor-VIII,dasatinib,bosutinib,and bortezomib(p<0.001).Survival analysis and receiver operating characteristic curves were validated using the E-MTAB-1980 cohort.Conclusions:SLC-related genes have predictive relevance in ccRCC and play roles in the immunological milieu.Our results provide insight into metabolic reprogramming in ccRCC and identify promising treatment targets for ccRCC.展开更多
Objective:To study explores the effect of HLEC on the secreted proteins of epithelial ovarian cancer(EOC)cells(SKOV3-PM4)with directional highly lymphatic metastasis.Methods:Supernatants of four groups of cultured cel...Objective:To study explores the effect of HLEC on the secreted proteins of epithelial ovarian cancer(EOC)cells(SKOV3-PM4)with directional highly lymphatic metastasis.Methods:Supernatants of four groups of cultured cells,namely,SKOV3(A),SKOV3+HLEC(B),SKOV3-PM4(C),SKOV3-PM4+HLEC(D),were collected,and their proteins were detected by antibody arrays and iTRAQ-2D-LC-MALDITOF/TOF/MS.Significantly differential proteins were further analyzed via bioinformatics and validated in human serums and cell media via ELISA.Results:Results of antibody arrays and mass spectrometry demonstrated that GRN and VEGFA were upregulated in group C(compared with group A),whereas IGFBP7 and SPARC were downregulated in group D(compared with group C).Comprehensive bioinformatics analysis results showed that IGFBP7 and VEGFA were closely linked to each other.Further validation with serums showed statistical significance in VEGFA and IGFBP7 levels among groups of patients with ovarian cancers,benign tumors,and control groups.Two proteins were upegulated in the first group.VEGFA in the control group was downregulated.For IGFBP,upregulation in the control group and down-regulation in the first group were also observed.Conclusion:The HLEC microenvironment is closely associated with directional metastasis to lymph nodes and with differential proteins including cell stromal proteins and adhesion factors.The upregulation of VEGFA and GRN and the downregulation of SPARC and IGFBP7 are closely associated with directional metastasis to lymph nodes in EOC cells.展开更多
Objective:To investigate the expression change of human leukocyte antigen(HLA) class I on human peripheral blood mononuclear cells(PBMCs) at both mRNA and protein levels,and to evaluate its roles in the development of...Objective:To investigate the expression change of human leukocyte antigen(HLA) class I on human peripheral blood mononuclear cells(PBMCs) at both mRNA and protein levels,and to evaluate its roles in the development of colorectal cancer(CRC).Methods:In the present study,50 patients with CRC,35 patients with benign colorectal lesion and 42 healthy volunteers were enrolled.Expression levels of HLA class I mRNA and protein were determined using real-time quantitative reverse transcription PCR(RT-PCR) and flow cytometry analysis,respectively.Results:The expression levels of HLA class I mRNA and proteins were not influenced by age and gender.The relative ratios of HLA class I mRNA were 0.99±0.27 in healthy controls,0.76±0.19 in benign patients,and 0.48±0.21 in CRC patients.Mean fluorescence intensities of HLA class I were 145.58±38.14 in healthy controls,102.05±35.98 in benign patients and 87.44±34.01 in CRC patients.HLA class I on PBMCs was significantly down-regulated at both mRNA and protein levels in patients with stage III and IV CRC.CRC patients with lymph node metastasis also showed a decreased HLA class I expression at protein level.Conclusion: HLA class I expressions on PBMCs are associated with staging of CRC and lymph node metastasis. Monitoring the expression of HLA class I on PBMCs may provide useful information for diagnosis and metastasis judgement of CRC.展开更多
Objective:Clinical practice guidelines recommend open adrenalectomy(OA)for large pheochromocytoma(LPCC)>6 cm in size.Although laparoscopic adrenalectomy(LA)for the treatment of LPCC has been reported,its role remai...Objective:Clinical practice guidelines recommend open adrenalectomy(OA)for large pheochromocytoma(LPCC)>6 cm in size.Although laparoscopic adrenalectomy(LA)for the treatment of LPCC has been reported,its role remains unclear.This study aimed to compare the effectiveness of LA and OA,and summary the surgical treatment experience.Methods:Data concerning LPCC,from January 2010 to June 2019 of a single institution,were retrospectively reviewed.Altogether 82 patients with a tumor larger than 6 cm were included(52 patients in LA group and 30 patients in OA group).Groups were balanced by propensity score matching(PSM)into 15 pairs.Patients’demographics,preoperative characteristics,and prognosis were analyzed.Results:Before PSM,the OA group had larger tumor sizes(median[interquartile range,IQR]:8.9[7.3-10.3]vs.7.2[6.7-8.0]cm;p=0.000)and higher vanillylmandelic acid level(median[IQR]:114.3[67.8-326.4]vs.66.6[37.8-145.8]μmol/24 h;p=0.004)and needed a higher cumulative dose of prazosin(median[IQR]:83.5[37.0-154.0]vs.38.0[21.0-81.0]mg;p=0.028).After PSM,the baseline data showed no significant differences between both groups.The LA group had relatively more stable blood pressure in surgery,with a lower fluctuation of systolic blood pressure(mean±standard deviation[SD]:70.9±25.1 vs.107.4±46.2 mmHg,p=0.012)and a lower percentage of hemodynamic instability(46.7%vs.86.7%,p=0.020).The LA group had shorter postoperative hospital stays(mean±SD:6.4±2.7 vs.10.1±3.4 days;p=0.003)than the OA group.Differences regarding metastasis rate(6.7%vs.0,p=1.000)were not statistically significant between LA and OA groups.The median(IQR)follow-up time of 82 patients was 72.5(47.0-103.5)months.Binary logistic regression showed that right-side tumors or those>8 cm in size were independent risk factors of OA.Conclusion:LA is a safe,minimally invasive procedure for LPCC and has relatively better perioperative characteristics in large medical centers.Patients with tumors on the right side or larger than 8 cm are more likely to undergo OA initially.展开更多
In conventional corrosion-resistant alloys,precipitation usually reduces corrosion resistance severely by weakening passive films locally.In this work,we found that the aging-treated AlCoCrFeNi_(2.1)sam-ples,which hav...In conventional corrosion-resistant alloys,precipitation usually reduces corrosion resistance severely by weakening passive films locally.In this work,we found that the aging-treated AlCoCrFeNi_(2.1)sam-ples,which have abundant nanosized L 12 and body-centered cubic(BCC)precipitates in the lamellar face-centered cubic(FCC)and B2 phases,displayed better corrosion resistance than solution-treated AlCoCrFeNi_(2.1)samples without precipitates.In the AlCoCrFeNi_(2.1)alloy,the FCC phase with L1_(2)precipi-tates and the B2 phase with BCC precipitates were protected by passive films enriched with Cr and Al elements,respectively.Moreover,the Al-rich passive film of the B2 phase was less stable than the Cr-rich passive film of the FCC phase,so B2 phase dissolved preferentially.The Cr-rich passive film of the FCC phase remained stable with the formation of Al-rich L1_(2)precipitates inside the phase because those precipitates with the size of∼5 nm were too small to affect the composition of the Cr-rich passive film.The formation of Cr-rich BCC precipitates within the B2 phase increased the content of the Al element inside the phase,improving the stability of Al-rich passive film on the B2 phase.Furthermore,BCC precip-itates with the size of∼30 nm were protected by Cr-rich passive film,which could inhibit the expansion of corrosion pits.Thus,the corrosion resistance of eutectic high-entropy alloy AlCoCrFeN 2.1 was unprece-dentedly enhanced by the precipitation of BCC precipitates.Our study could provide an attractive strategy for designing high-entropy alloys with excellent corrosion resistance and high strength.展开更多
A novel struvite crystallization method induced by bioelectrochemical acidolysis of magnesia (MgO) was investigated to recover phosphorus (P) from aqueous solution using a dual-chamber microbial electrolysis cell (DME...A novel struvite crystallization method induced by bioelectrochemical acidolysis of magnesia (MgO) was investigated to recover phosphorus (P) from aqueous solution using a dual-chamber microbial electrolysis cell (DMEC).Magnesium ion (Mg2+) in the anolyte was firstly confirmed to automatically migrate from the anode chamber to the cathode chamber,and then react with ammonium (NH4+) and phosphate (PO34-) in the catholyte to form struvite.Recovery efficiency of 17.8%-60.2% was obtained with the various N/P ratios in the catholyte.When MgO (low solubility under alkali conditions) was added into the anolyte,the bioelectrochemical acidolysis of MgO naturally took place and the released Mg2+ induced struvite crystallization in the cathode chamber for P recovery likewise.Besides,there was a strong linear positive correlation between the recovery efficiency and the MgO dosage (R2 =0.935),applied voltage (R2 =0.969) and N/P ratio (R2 =0.905).Increasing the applied voltage was found to enhance the P recovery via promoting the MgO acidolysis and the released Mg2+ migration,while increasing the N/P ratio in the catholyte enhanced the P recovery via promoting the struvite crystallization.Moreover,the electrochemical performance of the system was promoted due to more stable anolyte pH and lower pH gradient between the two chambers.Current density was promoted by 10%,while the COD removal efficiency was improved from 78.2% to 91.8% in the anode chamber.展开更多
This research aims to change the phenolic fractions and the bioactivities of quinoa by solid-state fermentation(SSF)with the edible fungus Monascus anka(M.anka).The contents of protein and fat increased in the ferment...This research aims to change the phenolic fractions and the bioactivities of quinoa by solid-state fermentation(SSF)with the edible fungus Monascus anka(M.anka).The contents of protein and fat increased in the fermented product,while the carbohydrate decreased.After 6-day fermentation with M.anka,the amount of phenolic compound reached the highest level.The majority of phenolic forms in fermented quinoa were phenolic acids,mainly ferulic acid,protocatechuic acid and p-hydroxybenzoic acid.Ultrahigh-performance liquid chromatography-mass spectrometry(UHPLC-MS)results showed that SSF was an effective method to transform quinoa phenolic compounds.Free fractions could be rapidly absorbed in the small intestine,suggesting that SSF with M.anka was a useful method to enhance bioavailable antioxidants.Antioxidant ability in vitro results showed that phenolic fractions from fermented quinoa were greater than the unfermented quinoa,and a significant cellular antioxidant activity(CAA)increment of 135%was obtained in the free phenolic fraction of fermented quinoa.Moreover,α-amylase andα-glucosidase inhibition activities were enhanced with fermentation.Correlation matrix analysis revealed that most of the free phenolic compounds showed strong positive correlations with antioxidant activities and digestive enzyme activities.Consequently,fermentation with M.anka was a particularly promising method to enhance the bioactivity of quinoa.展开更多
基金supported by the National Natural Science Foundation of China (32202051)the Shanghai Sailing Program (21YF1431800, 20YF1433400)+1 种基金Shanghai Agriculture Applied Technology Development Program, China (2021-02-08-0012-F00780 )the National Key R&D Program of China (2022YFF1100104, 2023YFF1103404)。
文摘Fermentation substrates of rice with different milling degrees(MDs) were prepared and fermented with human feces to compare their fermentation properties and effects on gut microbiota.MD 0s,MD 5s and MD 60s represented brown rice,moderately-milled rice and white rice,respectively.After in vitro fermentation,the MD 5s group showed higher starch utilization,compared with the MD 0s and 60s groups evaluated by Fourier transform infrared spectrometer,and confocal laser scanning microscope.Effects of fermentation substrates of rice with different MDs on gut microbiota were evaluated by 16S rDNA sequencing.All the sample groups reduced the pH and produced short-chain fatty acids(SCFAs) and branched-chain fatty acids.The MD 5s group exhibited higher α-diversity than the MD 0s and 60s groups.Abundances of Phascolarctobacterium,Blautia and norank_f_Ruminococcaceae were higher in the MD 0s and 5s groups,compared with the MD 60s group.These bacteria were also positively correlated with the SCFAs production via Spearman correlation analysis.In vitro culture assay revealed that fermentation substrates of MD 0s and 5s promoted the growth of two probiotics(Akkermansia muciniphila and Bifidobacterium adolescentis).Our results showed that moderate milling might be an appropriate way to produce rice products with richer nutrients and better fermentation properties.
基金financed by the National Natural Science Foundation(Grant Number 81874058 to Jianping Zhang).
文摘Background:Gastric cancer(GC)is a malignancy with the worst prognosis that seriously threatens human health,especially in East Asia.Apolipoprotein C1(apoc1)belongs to the apolipoprotein family.In addition,apoc1 has been associated with various tumors.However,its role in GC remains unclear.Methods:Firstly,we quantified its expression in GC and adjacent tumor tissues,using The Cancer Genome Atlas(TCGA).Next,we assessed cell invasion and migration abilities.Finally,we revealed the role of apoc1 in the tumor microenvironment(TME),immune cell infiltration and drug sensitivity.Results:Firstly,in TCGA database,it has been shown that elevated expression of apoc1 was identified in various cancers,including GC,then we found that high expression of apoc1 was significantly correlated with poor prognosis in GC.Histologically,apoc1 expression is proportional to grade,cancer stage,and T stage.The experimental results showed that apoc1 promoted cell invasion and migration.Then GO,KEGG,and GSEA pathway analyses indicated that apoc1 may be involved in the WNT pathway and immune regulation.Furthermore,we found out the tumor-infiltrating immune cells related to apoc1 in the tumor microenvironment(TME)using TIMER.Finally,we investigated the correlation between apoc1 expression and drug sensitivity,PD-1 and CTLA-4 therapy.Conclusions:These results suggest that apoc1 participates in the evolution of GC,and may represent a potential target for detection and immunotherapy in GC.
基金supported by the National Natural Science Foundation of China[Grant Numbers 82170769,81900684,and 81870512].
文摘Background:Clear-cell renal cell carcinoma(ccRCC)is the most common malignant kidney cancer.However,the tumor microenvironment and crosstalk involved in metabolic reprogramming in ccRCC are not well-understood.Methods:We used The Cancer Genome Atlas to obtain ccRCC transcriptome data and clinical information.The EMTAB-1980 cohort was used for external validation.The GENECARDS database contains the first 100 solute carrier(SLC)-related genes.The predictive value of SLC-related genes for ccRCC prognosis and treatment was assessed using univariate Cox regression analysis.An SLC-related predictive signature was developed through Lasso regression analysis and used to determine the risk profiles of patients with ccRCC.Patients in each cohort were separated into high-and low-risk groups based on their risk scores.The clinical importance of the signature was assessed through survival,immune microenvironment,drug sensitivity,and nomogram analyses using R software.Results:SLC25A23,SLC25A42,SLC5A1,SLC3A1,SLC25A37,SLC5A6,SLCO5A1,and SCP2 comprised the signatures of the eight SLCrelated genes.Patients with ccRCC were separated into high-and low-risk groups based on the risk value in the training and validation cohorts;the high-risk group had a significantly worse prognosis(p<0.001).The risk score was an independent predictive indicator of ccRCC in the two cohorts according to univariate and multivariate Cox regression(p<0.05).Analysis of the immune microenvironment showed that immune cell infiltration and immune checkpoint gene expression differed between the two groups(p<0.05).Drug sensitivity analysis showed that compared to the low-risk group,the high-risk group was more sensitive to sunitinib,nilotinib,JNK-inhibitor-VIII,dasatinib,bosutinib,and bortezomib(p<0.001).Survival analysis and receiver operating characteristic curves were validated using the E-MTAB-1980 cohort.Conclusions:SLC-related genes have predictive relevance in ccRCC and play roles in the immunological milieu.Our results provide insight into metabolic reprogramming in ccRCC and identify promising treatment targets for ccRCC.
基金supported by the National Natural Science Foundation of China (Grant No. 81060218)Guangxi Natural Science Foundation (Grant No. 2012GXNSFAA053157)
文摘Objective:To study explores the effect of HLEC on the secreted proteins of epithelial ovarian cancer(EOC)cells(SKOV3-PM4)with directional highly lymphatic metastasis.Methods:Supernatants of four groups of cultured cells,namely,SKOV3(A),SKOV3+HLEC(B),SKOV3-PM4(C),SKOV3-PM4+HLEC(D),were collected,and their proteins were detected by antibody arrays and iTRAQ-2D-LC-MALDITOF/TOF/MS.Significantly differential proteins were further analyzed via bioinformatics and validated in human serums and cell media via ELISA.Results:Results of antibody arrays and mass spectrometry demonstrated that GRN and VEGFA were upregulated in group C(compared with group A),whereas IGFBP7 and SPARC were downregulated in group D(compared with group C).Comprehensive bioinformatics analysis results showed that IGFBP7 and VEGFA were closely linked to each other.Further validation with serums showed statistical significance in VEGFA and IGFBP7 levels among groups of patients with ovarian cancers,benign tumors,and control groups.Two proteins were upegulated in the first group.VEGFA in the control group was downregulated.For IGFBP,upregulation in the control group and down-regulation in the first group were also observed.Conclusion:The HLEC microenvironment is closely associated with directional metastasis to lymph nodes and with differential proteins including cell stromal proteins and adhesion factors.The upregulation of VEGFA and GRN and the downregulation of SPARC and IGFBP7 are closely associated with directional metastasis to lymph nodes in EOC cells.
基金supported by Shandong Province Natural Science Foundation (No. Y2008C104)Shandong Province Science Foundation for Key Program (No. 2007GG20002027,2008GG2NS02016 and 2009GG10002043)
文摘Objective:To investigate the expression change of human leukocyte antigen(HLA) class I on human peripheral blood mononuclear cells(PBMCs) at both mRNA and protein levels,and to evaluate its roles in the development of colorectal cancer(CRC).Methods:In the present study,50 patients with CRC,35 patients with benign colorectal lesion and 42 healthy volunteers were enrolled.Expression levels of HLA class I mRNA and protein were determined using real-time quantitative reverse transcription PCR(RT-PCR) and flow cytometry analysis,respectively.Results:The expression levels of HLA class I mRNA and proteins were not influenced by age and gender.The relative ratios of HLA class I mRNA were 0.99±0.27 in healthy controls,0.76±0.19 in benign patients,and 0.48±0.21 in CRC patients.Mean fluorescence intensities of HLA class I were 145.58±38.14 in healthy controls,102.05±35.98 in benign patients and 87.44±34.01 in CRC patients.HLA class I on PBMCs was significantly down-regulated at both mRNA and protein levels in patients with stage III and IV CRC.CRC patients with lymph node metastasis also showed a decreased HLA class I expression at protein level.Conclusion: HLA class I expressions on PBMCs are associated with staging of CRC and lymph node metastasis. Monitoring the expression of HLA class I on PBMCs may provide useful information for diagnosis and metastasis judgement of CRC.
文摘Objective:Clinical practice guidelines recommend open adrenalectomy(OA)for large pheochromocytoma(LPCC)>6 cm in size.Although laparoscopic adrenalectomy(LA)for the treatment of LPCC has been reported,its role remains unclear.This study aimed to compare the effectiveness of LA and OA,and summary the surgical treatment experience.Methods:Data concerning LPCC,from January 2010 to June 2019 of a single institution,were retrospectively reviewed.Altogether 82 patients with a tumor larger than 6 cm were included(52 patients in LA group and 30 patients in OA group).Groups were balanced by propensity score matching(PSM)into 15 pairs.Patients’demographics,preoperative characteristics,and prognosis were analyzed.Results:Before PSM,the OA group had larger tumor sizes(median[interquartile range,IQR]:8.9[7.3-10.3]vs.7.2[6.7-8.0]cm;p=0.000)and higher vanillylmandelic acid level(median[IQR]:114.3[67.8-326.4]vs.66.6[37.8-145.8]μmol/24 h;p=0.004)and needed a higher cumulative dose of prazosin(median[IQR]:83.5[37.0-154.0]vs.38.0[21.0-81.0]mg;p=0.028).After PSM,the baseline data showed no significant differences between both groups.The LA group had relatively more stable blood pressure in surgery,with a lower fluctuation of systolic blood pressure(mean±standard deviation[SD]:70.9±25.1 vs.107.4±46.2 mmHg,p=0.012)and a lower percentage of hemodynamic instability(46.7%vs.86.7%,p=0.020).The LA group had shorter postoperative hospital stays(mean±SD:6.4±2.7 vs.10.1±3.4 days;p=0.003)than the OA group.Differences regarding metastasis rate(6.7%vs.0,p=1.000)were not statistically significant between LA and OA groups.The median(IQR)follow-up time of 82 patients was 72.5(47.0-103.5)months.Binary logistic regression showed that right-side tumors or those>8 cm in size were independent risk factors of OA.Conclusion:LA is a safe,minimally invasive procedure for LPCC and has relatively better perioperative characteristics in large medical centers.Patients with tumors on the right side or larger than 8 cm are more likely to undergo OA initially.
基金This work was financially supported by the Natural Science Foundation of Hebei Province(No.E2020202009)the Tianjin Science and Technology Plan Project(No.20JCQNJC00900)+3 种基金the Science and Technology Project of Hebei Education Department(No.QN2020150)the National Natural Science Foundation of China(No.52101011)the Central Funds Guiding the Local Science and Technology Development of Hebei Province(No.226Z1001G)the Central Funds Guiding the Local Science and Technology Development of Hebei Province(No.226Z1012G).
文摘In conventional corrosion-resistant alloys,precipitation usually reduces corrosion resistance severely by weakening passive films locally.In this work,we found that the aging-treated AlCoCrFeNi_(2.1)sam-ples,which have abundant nanosized L 12 and body-centered cubic(BCC)precipitates in the lamellar face-centered cubic(FCC)and B2 phases,displayed better corrosion resistance than solution-treated AlCoCrFeNi_(2.1)samples without precipitates.In the AlCoCrFeNi_(2.1)alloy,the FCC phase with L1_(2)precipi-tates and the B2 phase with BCC precipitates were protected by passive films enriched with Cr and Al elements,respectively.Moreover,the Al-rich passive film of the B2 phase was less stable than the Cr-rich passive film of the FCC phase,so B2 phase dissolved preferentially.The Cr-rich passive film of the FCC phase remained stable with the formation of Al-rich L1_(2)precipitates inside the phase because those precipitates with the size of∼5 nm were too small to affect the composition of the Cr-rich passive film.The formation of Cr-rich BCC precipitates within the B2 phase increased the content of the Al element inside the phase,improving the stability of Al-rich passive film on the B2 phase.Furthermore,BCC precip-itates with the size of∼30 nm were protected by Cr-rich passive film,which could inhibit the expansion of corrosion pits.Thus,the corrosion resistance of eutectic high-entropy alloy AlCoCrFeN 2.1 was unprece-dentedly enhanced by the precipitation of BCC precipitates.Our study could provide an attractive strategy for designing high-entropy alloys with excellent corrosion resistance and high strength.
基金supported by the National Key R&D Program of China(No.2017YFC0403400)the Fundamental Research Funds for the Central Universitiesthe Foundation of Key Laboratory of Yangtze River Water Environment,Ministry of Education(Tongji University),China(No.YRWEF201805)
文摘A novel struvite crystallization method induced by bioelectrochemical acidolysis of magnesia (MgO) was investigated to recover phosphorus (P) from aqueous solution using a dual-chamber microbial electrolysis cell (DMEC).Magnesium ion (Mg2+) in the anolyte was firstly confirmed to automatically migrate from the anode chamber to the cathode chamber,and then react with ammonium (NH4+) and phosphate (PO34-) in the catholyte to form struvite.Recovery efficiency of 17.8%-60.2% was obtained with the various N/P ratios in the catholyte.When MgO (low solubility under alkali conditions) was added into the anolyte,the bioelectrochemical acidolysis of MgO naturally took place and the released Mg2+ induced struvite crystallization in the cathode chamber for P recovery likewise.Besides,there was a strong linear positive correlation between the recovery efficiency and the MgO dosage (R2 =0.935),applied voltage (R2 =0.969) and N/P ratio (R2 =0.905).Increasing the applied voltage was found to enhance the P recovery via promoting the MgO acidolysis and the released Mg2+ migration,while increasing the N/P ratio in the catholyte enhanced the P recovery via promoting the struvite crystallization.Moreover,the electrochemical performance of the system was promoted due to more stable anolyte pH and lower pH gradient between the two chambers.Current density was promoted by 10%,while the COD removal efficiency was improved from 78.2% to 91.8% in the anode chamber.
基金This work was supported by Shanghai Agriculture Applied Technology Development Program,China(2021-02-08-00-12-F00780)the Shanghai Committee of Science and Technology,China(20DZ2202700)+1 种基金the Capacity-Building Project of Local Universities of SSTC(20060502100)the Shanghai Sailing Program(21YF1431800)and(20YF1433400).
文摘This research aims to change the phenolic fractions and the bioactivities of quinoa by solid-state fermentation(SSF)with the edible fungus Monascus anka(M.anka).The contents of protein and fat increased in the fermented product,while the carbohydrate decreased.After 6-day fermentation with M.anka,the amount of phenolic compound reached the highest level.The majority of phenolic forms in fermented quinoa were phenolic acids,mainly ferulic acid,protocatechuic acid and p-hydroxybenzoic acid.Ultrahigh-performance liquid chromatography-mass spectrometry(UHPLC-MS)results showed that SSF was an effective method to transform quinoa phenolic compounds.Free fractions could be rapidly absorbed in the small intestine,suggesting that SSF with M.anka was a useful method to enhance bioavailable antioxidants.Antioxidant ability in vitro results showed that phenolic fractions from fermented quinoa were greater than the unfermented quinoa,and a significant cellular antioxidant activity(CAA)increment of 135%was obtained in the free phenolic fraction of fermented quinoa.Moreover,α-amylase andα-glucosidase inhibition activities were enhanced with fermentation.Correlation matrix analysis revealed that most of the free phenolic compounds showed strong positive correlations with antioxidant activities and digestive enzyme activities.Consequently,fermentation with M.anka was a particularly promising method to enhance the bioactivity of quinoa.
