AIM To determine the effects of ω-3 fatty acids(ω-3FA) on the toll-like receptor 4(TLR4)/nuclear factor κB p56(NF-κBp56) signal pathway in the lungs of rats with severe acute pancreatitis(SAP).METHODS A total of 5...AIM To determine the effects of ω-3 fatty acids(ω-3FA) on the toll-like receptor 4(TLR4)/nuclear factor κB p56(NF-κBp56) signal pathway in the lungs of rats with severe acute pancreatitis(SAP).METHODS A total of 56 Sprague-Dawley rats were randomly divided into 4 groups: control group, SAP-saline group, SAP-soybean oil group and SAP-ω-3FA group. SAP was induced by the retrograde infusion of sodium taurocholate into the pancreatic duct. The expression of TLR4 and NF-κBp56 in the lungs was evaluated by immunohistochemistry and Western blot analysis. The levels of inflammatory cytokines interleukin-6 and tumor necrosis factor-alpha in the lungs were measured by enzyme-linked immunosorbent assay. RESULTS The expression of TLR4 and NF-κBp56 in lungs and of inflammatory cytokines in serum significantly increased in the SAP group compared with the control group(P < 0.05), but was significantly decreased in the ω-3FA group compared with the soybean oil group at 12 and 24 h(P < 0.05).CONCLUSION During the initial stage of SAP, ω-3FA can efficiently lower the inflammatory response and reduce lung injury by triggering the TLR4/NF-κBp56 signal pathway.展开更多
Objective:To study the effect of retention enema by caulis sargentodoxae enema combined with oral drugs on the immune inflammatory response and intestinal flora of UC patients. Methods: A total of 128 UC patients who ...Objective:To study the effect of retention enema by caulis sargentodoxae enema combined with oral drugs on the immune inflammatory response and intestinal flora of UC patients. Methods: A total of 128 UC patients who received treatment in the hospital between January 2013 and April 2017 were collected and divided into control group and observation group by random number table. Control group received conventional oral drug therapy, and the observation group received retention enema by caulis sargentodoxae enema combined with oral drug therapy. Before treatment and after 4 weeks of treatment, the differences in the levels of immunoglobulin and inflammatory response indexes in serum as well as intestinal flora levels in mucosa lesion tissue were compared between the two groups.Results: The differences in levels of immunoglobulin and inflammatory response indexes in serum as well as intestinal flora levels in mucosa lesion tissue were not statistically significant between the two groups of patients before treatment. After 4 weeks of treatment, IgA, IgG, IgM and IL-4 contents in serum as well as bifidobacterium and lactobacillus levels in mucosa lesion tissue of observation group were higher than those of control group while ESR, CRP, IL-6 and TNF-αcontents as well as E. coli level in mucosa lesion tissue were lower than those of control group. Conclusion: Retention enema by caulis sargentodoxae enema combined with oral drugs can effectively optimize the immune inflammatory state and balance the intestinal flora distribution in patients with UC.展开更多
基金Supported by Jinling Hospital Research Fund,No.2013064
文摘AIM To determine the effects of ω-3 fatty acids(ω-3FA) on the toll-like receptor 4(TLR4)/nuclear factor κB p56(NF-κBp56) signal pathway in the lungs of rats with severe acute pancreatitis(SAP).METHODS A total of 56 Sprague-Dawley rats were randomly divided into 4 groups: control group, SAP-saline group, SAP-soybean oil group and SAP-ω-3FA group. SAP was induced by the retrograde infusion of sodium taurocholate into the pancreatic duct. The expression of TLR4 and NF-κBp56 in the lungs was evaluated by immunohistochemistry and Western blot analysis. The levels of inflammatory cytokines interleukin-6 and tumor necrosis factor-alpha in the lungs were measured by enzyme-linked immunosorbent assay. RESULTS The expression of TLR4 and NF-κBp56 in lungs and of inflammatory cytokines in serum significantly increased in the SAP group compared with the control group(P < 0.05), but was significantly decreased in the ω-3FA group compared with the soybean oil group at 12 and 24 h(P < 0.05).CONCLUSION During the initial stage of SAP, ω-3FA can efficiently lower the inflammatory response and reduce lung injury by triggering the TLR4/NF-κBp56 signal pathway.
文摘Objective:To study the effect of retention enema by caulis sargentodoxae enema combined with oral drugs on the immune inflammatory response and intestinal flora of UC patients. Methods: A total of 128 UC patients who received treatment in the hospital between January 2013 and April 2017 were collected and divided into control group and observation group by random number table. Control group received conventional oral drug therapy, and the observation group received retention enema by caulis sargentodoxae enema combined with oral drug therapy. Before treatment and after 4 weeks of treatment, the differences in the levels of immunoglobulin and inflammatory response indexes in serum as well as intestinal flora levels in mucosa lesion tissue were compared between the two groups.Results: The differences in levels of immunoglobulin and inflammatory response indexes in serum as well as intestinal flora levels in mucosa lesion tissue were not statistically significant between the two groups of patients before treatment. After 4 weeks of treatment, IgA, IgG, IgM and IL-4 contents in serum as well as bifidobacterium and lactobacillus levels in mucosa lesion tissue of observation group were higher than those of control group while ESR, CRP, IL-6 and TNF-αcontents as well as E. coli level in mucosa lesion tissue were lower than those of control group. Conclusion: Retention enema by caulis sargentodoxae enema combined with oral drugs can effectively optimize the immune inflammatory state and balance the intestinal flora distribution in patients with UC.
基金Project supported by the UK Engineering and Physical Sciences Research Council(No.EP/E041965)the National Basic Research Program(973 Program)of China(No.2012CB719800)the Zhejiang Provincial Natural Science Foundation of China(No.LY17E080021)
