BACKGROUND: Cardiac arrest(CA) is a critical condition that is a concern to healthcare workers. Comparative studies on extracorporeal cardiopulmonary resuscitation(ECPR) and conventional cardiopulmonary resuscitation(...BACKGROUND: Cardiac arrest(CA) is a critical condition that is a concern to healthcare workers. Comparative studies on extracorporeal cardiopulmonary resuscitation(ECPR) and conventional cardiopulmonary resuscitation(CCPR) technologies have shown that ECPR is superior to CCPR. However, there is a lack of studies that compare the protective effects of these two resuscitative methods on organs. Therefore, we aim to perform experiments in swine models of ventricular fibrillation-induced CA to study whether the early application of ECPR has advantages over CCPR in the lung injury and to explore the protective mechanism of ECPR on the post-resuscitation pulmonary injury.METHODS: Sixteen male swine were randomized to CCPR(CCPR;n=8;CCPR alone) and ECPR(ECPR;n=8;extracorporeal membrane oxygenation with CCPR) groups, with the restoration of spontaneous circulation at 6 hours as an endpoint. RESULTS: For the two groups, the survival rates between the two groups were not statistically significant(P>0.05), the blood and lung biomarkers were statistically significant(P<0.05), and the extravascular lung water and pulmonary vascular permeability index were statistically significant(P<0.01). Compared with the ECPR group, electron microscopy revealed mostly vacuolated intracellular alveolar type II lamellar bodies and a fuzzy lamellar structure with widening and blurring of the blood-gas barrier in the CCPR group.CONCLUSIONS: ECPR may have pulmonary protective effects, possibly related to the regulation of alveolar surface-active proteins and mitigated oxidative stress response postresuscitation.展开更多
Objective:To investigate the effect of minocycline combined with periocline on patients with periodontitis, and analyze the effect of inflammatory factors, ALP and NO levels in the gingival sulcus fluid.Methods:A tota...Objective:To investigate the effect of minocycline combined with periocline on patients with periodontitis, and analyze the effect of inflammatory factors, ALP and NO levels in the gingival sulcus fluid.Methods:A total of 100 cases of patients with periodontitis admitted in our hospital from December 2016 to May 2017, with a total of 128 teeth. They were divided into control group and observation group by the random number method, 64 teeth in each group. Patients in both groups were given conventional supragingival scaling and subgingival curettage therapy. The control group patients were given minocycline ointment in periodontal pockets until overflow, once a week;the observation group was given periocline treatment on the basis of control group. Oral administration 2 capsules each time, 2 times each day. The two groups were both treated for 4 weeks. The general indicators of teeth in both groups were compared. The levels of inflammatory factors, ALP and NO in gingival crevicular fluid of the two groups were detected before and after the treatment.Results: Before treatment, there was no significant difference in the levels of PLI, PD, SBI, AL and TNF-α, CRP, ALP and NO in the gingival crevicular fluid of the both groups. After treatment, the levels of PLI, PD, SBI, AL and TNF-α, CRP, ALP and NO in the gingival crevicular fluid of the two groups were significantly decreased than those before the treatment, and the levels of PLI, PD, SBI, AL and TNF-α, CRP, ALP and NO in gingival crevicular fluid after treatment in the observation group were all significantly lower than that in the control group after treatment, the differences were statistically significant.Conclusion: Minocycline combined with periocline can improve the periodontal condition and gingival crevicular fluid inflammatory state in patients with periodontitis, reduce the activity of ALP and NO. It has the effect of antiseptic and induced osteogenesis and improving the clinical effect.展开更多
Objective: To explore the effect of thymalfasin on coagulation index, immune function, PCT and sTREM-1 levels in patients with community acquired pneumonia. Methods: 160 patients with community acquired pneumonia admi...Objective: To explore the effect of thymalfasin on coagulation index, immune function, PCT and sTREM-1 levels in patients with community acquired pneumonia. Methods: 160 patients with community acquired pneumonia admitted in our hospital from June 2016-June 2017 were divided into the control group and the observation group according to the random number table method, each 80 cases. The control group was given conventional treatment, while the observation group was given thymalfasin on the basis of conventional treatment. The levels of D-D, FBG, complement C3, C4, T lymphocyte subsets, PCT and sTREM-1 were detected before and after treatment. Results: Before treatment, there was no statistical difference between the serum levels of D-D, FBG, C3, C4, CD3+,CD4+,CD8+,CD4+/CD8+, PCT and sTREM-1 in the two groups of patients, and P>0.05. After treatment, the levels of CD3+, CD4+and CD4+/CD8+ in the control group and the observation group were significantly increased, and the levels of D-D, FBG, CD8+, PCT and sTREM-1 were significantly decreased, and the level of CD3+, CD4+ and CD4+/CD8+ in the observation group was significantly higher than that of the control group after treatment, and the D-D, FBG, CD8+, PCT and sTREM-1 levels were significantly lower than those in the control group, the difference was statistically significant, P<0.05. Conclusion: Thymalfasin has a certain clinical effect in the treatment of patients with community acquired pneumonia. It can significantly improve the blood coagulation function, enhance the immune ability and alleviate the inflammatory state.展开更多
Background： Acute kidney injury （AKI） frequently occurs in cardiopulmonary resuscitation patients. Studies comparing the effects of extracorporeal membrane oxygenation （ECMO） with conventional cardiopuhnonary res...Background： Acute kidney injury （AKI） frequently occurs in cardiopulmonary resuscitation patients. Studies comparing the effects of extracorporeal membrane oxygenation （ECMO） with conventional cardiopuhnonary resuscitation （CCPR） on AKI were rare. This study aimed to compare the effects of ECMO with those of CCPR on survival rate and AKI and explore the underlying mechanisms in a swine model of cardiac arrest （CA）. Methods： Sixteen male pigs were treated with ventricular fibrillation to establish CA model and then underwent CCPR （CCPR group, n = 8） or ECMO during cardiopulmonary resuscitation （ECPR group, n = 8）. The study endpoints were 6 h after return of spontaneous circulation （ROSC） or death. Serum and urine samples were collected at baseline and during the 6 h after ROSC. The biomarkers of AKI were detected by enzyme-linked immunosorbent assay. The apoptosis of renal tubular epithelial cells was discovered by transmission electron microscope （TEM） and terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Apoptosis-related genes were detected by immune-staining and Western blotting. Data were compared by Student＇s t-test. Results： All pigs in ECPR group were successfully resuscitated with a higher 6-h survival rate （8/8） compared to CCPR group （6/8）. The expressions ofAKl biomarkers including neutrophil gelatinase-associated lipocalin （NGAL）, tissue inhibitor ofmetalloproteinase2 （TIMP2）, insulin-like growth factor-binding protein 7 （IGFBP7）, liver fatty acid-binding protein （LFABP）, and kidney injury molecule l （Kim-1） were all increased along with the time after ROSC in both groups and lower in ECPR group compared with CCPR group. Especially, products of urinary T1MP and IGFBP levels （TIMP＊IGFBP） were significantly lower at ROSC4 （0.58 ± 0.10 ng^2/ml^2 vs. 1.18 ± 0.38 ng^2/ml^2, t = 4.33, P =0.003） and ROSC6 （1.79 ±0.45 ng2^/ml^2 vs. 3.00 ±0.44 ng^2/ml^2, t = 5.49, P 〈 0.001）; urinary LFABP was significantly lower at ROSC6 （0.74 ± 0.06 pg/ml vs. 0.85 4±0.11 pg/ml, t = 2.41, P = 0.033）; and urinary Kim-1 was significantly lower at ROSC4 （0.66 ± 0.09 pg/ml vs. 0.83 ± 0.06 pg/ml, t = 3.99, P = 0.002） and ROSC6 （0.73 ± 0.12 pg/ml vs. 0.89 ± 0.08 pg/ml, t = 2.82, P = 0.016）. Under light microscope and TEM, the morphological injures in renal tissues were found to be improved in ECPR group. Moreover, apoptosis was also alleviated in ECPR group. Conclusions： Compared with CCPR, ECMO improves survival rate and alleviates AKI in a swine model of CA. The mechanism of which might be via downregulating AKI biomarkers and apoptosis in kidney.展开更多
The physical properties of ZrxTi1-x（x=0.0, 0.33, 0.5, 0.67, 0.75 and 1.00） alloys were sinmlated by virtual crystal approximation （VCA） methods which is generally used for disordered solid solutions modeling. The ...The physical properties of ZrxTi1-x（x=0.0, 0.33, 0.5, 0.67, 0.75 and 1.00） alloys were sinmlated by virtual crystal approximation （VCA） methods which is generally used for disordered solid solutions modeling. The elastic constant, electronic structure and thermal Equation of state （EOS） of disor- dered ZrxTi1-x alloys under pressure are investigated by plane-wave pseudo-potentia1 method. Our simulations reveal increasement of variations of the calculated equilibrium volumes and decrease- ment of Bulk modulus as a function of the alloy compositions. Lattice parameters a and c of alloys with differentZr concentrations decrease linearly with pressure increasing, but the c/avalues are increasing as pressure increases, indicating no phase transitions under pressure from 0 GPa to 100 GPa. The elastic constants and the Bulk modulus to the Shear modulus ratios （B/G） indicate good ductility of Zr, Zr0.33 Ti0.67 Zr0.5Ti0.5, Zr0.75Ti0.25 and Ti, but the Zr0.67Ti0.33 alloy is brittle under 0 K and 0 GPa. The metallic behavior of these alloys was also proved by analyzing partial and total DOS.展开更多
文摘BACKGROUND: Cardiac arrest(CA) is a critical condition that is a concern to healthcare workers. Comparative studies on extracorporeal cardiopulmonary resuscitation(ECPR) and conventional cardiopulmonary resuscitation(CCPR) technologies have shown that ECPR is superior to CCPR. However, there is a lack of studies that compare the protective effects of these two resuscitative methods on organs. Therefore, we aim to perform experiments in swine models of ventricular fibrillation-induced CA to study whether the early application of ECPR has advantages over CCPR in the lung injury and to explore the protective mechanism of ECPR on the post-resuscitation pulmonary injury.METHODS: Sixteen male swine were randomized to CCPR(CCPR;n=8;CCPR alone) and ECPR(ECPR;n=8;extracorporeal membrane oxygenation with CCPR) groups, with the restoration of spontaneous circulation at 6 hours as an endpoint. RESULTS: For the two groups, the survival rates between the two groups were not statistically significant(P>0.05), the blood and lung biomarkers were statistically significant(P<0.05), and the extravascular lung water and pulmonary vascular permeability index were statistically significant(P<0.01). Compared with the ECPR group, electron microscopy revealed mostly vacuolated intracellular alveolar type II lamellar bodies and a fuzzy lamellar structure with widening and blurring of the blood-gas barrier in the CCPR group.CONCLUSIONS: ECPR may have pulmonary protective effects, possibly related to the regulation of alveolar surface-active proteins and mitigated oxidative stress response postresuscitation.
文摘Objective:To investigate the effect of minocycline combined with periocline on patients with periodontitis, and analyze the effect of inflammatory factors, ALP and NO levels in the gingival sulcus fluid.Methods:A total of 100 cases of patients with periodontitis admitted in our hospital from December 2016 to May 2017, with a total of 128 teeth. They were divided into control group and observation group by the random number method, 64 teeth in each group. Patients in both groups were given conventional supragingival scaling and subgingival curettage therapy. The control group patients were given minocycline ointment in periodontal pockets until overflow, once a week;the observation group was given periocline treatment on the basis of control group. Oral administration 2 capsules each time, 2 times each day. The two groups were both treated for 4 weeks. The general indicators of teeth in both groups were compared. The levels of inflammatory factors, ALP and NO in gingival crevicular fluid of the two groups were detected before and after the treatment.Results: Before treatment, there was no significant difference in the levels of PLI, PD, SBI, AL and TNF-α, CRP, ALP and NO in the gingival crevicular fluid of the both groups. After treatment, the levels of PLI, PD, SBI, AL and TNF-α, CRP, ALP and NO in the gingival crevicular fluid of the two groups were significantly decreased than those before the treatment, and the levels of PLI, PD, SBI, AL and TNF-α, CRP, ALP and NO in gingival crevicular fluid after treatment in the observation group were all significantly lower than that in the control group after treatment, the differences were statistically significant.Conclusion: Minocycline combined with periocline can improve the periodontal condition and gingival crevicular fluid inflammatory state in patients with periodontitis, reduce the activity of ALP and NO. It has the effect of antiseptic and induced osteogenesis and improving the clinical effect.
文摘Objective: To explore the effect of thymalfasin on coagulation index, immune function, PCT and sTREM-1 levels in patients with community acquired pneumonia. Methods: 160 patients with community acquired pneumonia admitted in our hospital from June 2016-June 2017 were divided into the control group and the observation group according to the random number table method, each 80 cases. The control group was given conventional treatment, while the observation group was given thymalfasin on the basis of conventional treatment. The levels of D-D, FBG, complement C3, C4, T lymphocyte subsets, PCT and sTREM-1 were detected before and after treatment. Results: Before treatment, there was no statistical difference between the serum levels of D-D, FBG, C3, C4, CD3+,CD4+,CD8+,CD4+/CD8+, PCT and sTREM-1 in the two groups of patients, and P>0.05. After treatment, the levels of CD3+, CD4+and CD4+/CD8+ in the control group and the observation group were significantly increased, and the levels of D-D, FBG, CD8+, PCT and sTREM-1 were significantly decreased, and the level of CD3+, CD4+ and CD4+/CD8+ in the observation group was significantly higher than that of the control group after treatment, and the D-D, FBG, CD8+, PCT and sTREM-1 levels were significantly lower than those in the control group, the difference was statistically significant, P<0.05. Conclusion: Thymalfasin has a certain clinical effect in the treatment of patients with community acquired pneumonia. It can significantly improve the blood coagulation function, enhance the immune ability and alleviate the inflammatory state.
基金The study was supported by me National Natural Science Foundation of China （No. 81372025） and the 2015 Annual Special Cultivation and Development Project for the Technology Innovation Base of the Beijing Key Laboratory Cardiopulmonary Cerebral Resuscitation （No.Z 151100001615056）.
文摘Background： Acute kidney injury （AKI） frequently occurs in cardiopulmonary resuscitation patients. Studies comparing the effects of extracorporeal membrane oxygenation （ECMO） with conventional cardiopuhnonary resuscitation （CCPR） on AKI were rare. This study aimed to compare the effects of ECMO with those of CCPR on survival rate and AKI and explore the underlying mechanisms in a swine model of cardiac arrest （CA）. Methods： Sixteen male pigs were treated with ventricular fibrillation to establish CA model and then underwent CCPR （CCPR group, n = 8） or ECMO during cardiopulmonary resuscitation （ECPR group, n = 8）. The study endpoints were 6 h after return of spontaneous circulation （ROSC） or death. Serum and urine samples were collected at baseline and during the 6 h after ROSC. The biomarkers of AKI were detected by enzyme-linked immunosorbent assay. The apoptosis of renal tubular epithelial cells was discovered by transmission electron microscope （TEM） and terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Apoptosis-related genes were detected by immune-staining and Western blotting. Data were compared by Student＇s t-test. Results： All pigs in ECPR group were successfully resuscitated with a higher 6-h survival rate （8/8） compared to CCPR group （6/8）. The expressions ofAKl biomarkers including neutrophil gelatinase-associated lipocalin （NGAL）, tissue inhibitor ofmetalloproteinase2 （TIMP2）, insulin-like growth factor-binding protein 7 （IGFBP7）, liver fatty acid-binding protein （LFABP）, and kidney injury molecule l （Kim-1） were all increased along with the time after ROSC in both groups and lower in ECPR group compared with CCPR group. Especially, products of urinary T1MP and IGFBP levels （TIMP＊IGFBP） were significantly lower at ROSC4 （0.58 ± 0.10 ng^2/ml^2 vs. 1.18 ± 0.38 ng^2/ml^2, t = 4.33, P =0.003） and ROSC6 （1.79 ±0.45 ng2^/ml^2 vs. 3.00 ±0.44 ng^2/ml^2, t = 5.49, P 〈 0.001）; urinary LFABP was significantly lower at ROSC6 （0.74 ± 0.06 pg/ml vs. 0.85 4±0.11 pg/ml, t = 2.41, P = 0.033）; and urinary Kim-1 was significantly lower at ROSC4 （0.66 ± 0.09 pg/ml vs. 0.83 ± 0.06 pg/ml, t = 3.99, P = 0.002） and ROSC6 （0.73 ± 0.12 pg/ml vs. 0.89 ± 0.08 pg/ml, t = 2.82, P = 0.016）. Under light microscope and TEM, the morphological injures in renal tissues were found to be improved in ECPR group. Moreover, apoptosis was also alleviated in ECPR group. Conclusions： Compared with CCPR, ECMO improves survival rate and alleviates AKI in a swine model of CA. The mechanism of which might be via downregulating AKI biomarkers and apoptosis in kidney.
基金The first author greatly thanks Dr. Ruo- Xu Gu for his help with English language editing. The work was supported by Postdoctoral Science Foundation of China （Grant No. 2013M541596）, Jiangsu Planned Projects for Postdoctoral Re- search Funds （Grant No. 1202105C）, National Basic Research Pro- gram of China （Grant No. 2010CB731600）, the National Natural Science Foundation of China （Grant Nos. 51209080, 10776022, and 20773085）, China Academy of Engineering Physics （CAEP）, and China Postdoctoral Science Foundation funded project （Grant No. 2012M511192）.
文摘The physical properties of ZrxTi1-x（x=0.0, 0.33, 0.5, 0.67, 0.75 and 1.00） alloys were sinmlated by virtual crystal approximation （VCA） methods which is generally used for disordered solid solutions modeling. The elastic constant, electronic structure and thermal Equation of state （EOS） of disor- dered ZrxTi1-x alloys under pressure are investigated by plane-wave pseudo-potentia1 method. Our simulations reveal increasement of variations of the calculated equilibrium volumes and decrease- ment of Bulk modulus as a function of the alloy compositions. Lattice parameters a and c of alloys with differentZr concentrations decrease linearly with pressure increasing, but the c/avalues are increasing as pressure increases, indicating no phase transitions under pressure from 0 GPa to 100 GPa. The elastic constants and the Bulk modulus to the Shear modulus ratios （B/G） indicate good ductility of Zr, Zr0.33 Ti0.67 Zr0.5Ti0.5, Zr0.75Ti0.25 and Ti, but the Zr0.67Ti0.33 alloy is brittle under 0 K and 0 GPa. The metallic behavior of these alloys was also proved by analyzing partial and total DOS.
