BACKGROUND Microwave ablation(MWA)is an effective treatment option for patients with primary liver cancer.However,it has been reported that the MWA procedure induces a hepatic inflammatory response and injury,which ma...BACKGROUND Microwave ablation(MWA)is an effective treatment option for patients with primary liver cancer.However,it has been reported that the MWA procedure induces a hepatic inflammatory response and injury,which may negatively affect the efficacy of MWA.As such,the discovery of reliable markers to monitor the patient’s response to MWA is needed.Golgi protein 73(GP73)has been shown to be associated with chronic liver disease.To date,the potential value of serum GP73 in the dynamic monitoring during MWA of liver cancer remains unclear.AIM To examine the effects of MWA on the serum levels of GP73 in patients with primary liver cancer.METHODS A total of 150 primary liver cancer patients with a single small lesion(≤3 cm in diameter)were retrospectively enrolled spanning the period between January 2016 and October 2018.All of the patients received MWA for the treatment of primary liver cancer.Serum GP73,alpha-fetoprotein(AFP),and widely used liver biochemical indicators[serum albumin,total bilirubin(TBIL),alanine aminotransferase(ALT),and aspartate aminotransferase(AST)]were compared before MWA and at different time points,including 1,2,and 4 wk following the ablation procedure.RESULTS Complete tumor ablation was achieved in 95.33%of the patients at 1 mo after MWA.The 1-,2-,and 3-year disease-free survival rates were 74.67%,59.33%,and 54.00%,respectively.The serum AFP levels were significantly decreased at 1,2,and 4 wk after MWA;they returned to the normal range at 12 wk after MWA;and they remained stable thereafter during follow-up in those cases without recurrence.In contrast,the serum GP73 levels were significantly increased at 1 and 2 wk after MWA.The serum GP73 levels reached the peak at 2 wk after MWA,started to decline after hepatoprotective treatment with glycyrrhizin and reduced glutathione,and returned to the pretreatment levels at 12 and 24 wk after MWA.Notably,the changes of serum GP73 in response to MWA were similar to those of TBIL,ALT,and AST.CONCLUSION Serum GP73 is markedly increased in response to MWA of liver cancer.Thus,serum GP73 holds potential as a marker to monitor MWA-induced inflammatory liver injury in need of amelioration.展开更多
Objective:Pain is a prevalent and distressing symptom in patients with cancer which has a significant impact on patients’physical health,psychological well-being,and quality of life(QOL).Deficiency of pain care,inade...Objective:Pain is a prevalent and distressing symptom in patients with cancer which has a significant impact on patients’physical health,psychological well-being,and quality of life(QOL).Deficiency of pain care,inadequate pain communication,and reluctance toward pain medication contribute to the difficulties in improving the situation of patients with cancer pain.Fortunately,the integration of self-management and professional healthcare provides new opportunities for patients with cancer pain.Methods:A two-arm randomized controlled trial will be conducted to assess the effectiveness of self-management application compared with routine care among patients with cancer pain.Based on Orem’s self-care theory and Naylor’s transitional care theory,cancer pain self-management application“Pain-housekeeper”was developed with dual client:patient client and medical client.A total of 120 patients with cancer pain are recruited from two university-affiliated tertiary public hospitals in China.They are randomly assigned to the control group and the intervention group.The patients in the control group get routine care while the patients in the intervention group get routine care and Pain-housekeeper App.The intervention is delivered by registered doctors,nurses,and postgraduate nursing students specialized in pain care.Effect measurements for both groups are carried out with questionnaires at baseline(T0),after 4 weeks(T1)and after 12 weeks(T2).The primary outcomes include pain condition,self-efficacy,and QOL.The secondary outcomes include pain medication adherence,satisfaction,anxiety,and depression.Compliance of data submission,economic evaluation,usage,and clinical data are also considered in the test.Data for the evaluation are gathered continuously during the study period.Discussion:This article provides insight into the effectiveness of a theory-based self-management mobile application intervention delivered to patients with uncontrolled cancer pain.The results are used to help patients to improve cancer pain control,self-efficacy,and QOL.Conclusions:The findings of this article provide evidence to support the implementation of an innovative and easily accessible intervention that enhances health outcomes.展开更多
For a scalar integrable model,it is generally believed that the solitons interact with each other elastically,for instance,multi-bright solitons from the nonlinear Schrodinger equation and the Korteweg-de Vries equati...For a scalar integrable model,it is generally believed that the solitons interact with each other elastically,for instance,multi-bright solitons from the nonlinear Schrodinger equation and the Korteweg-de Vries equation,etc.We obtain double-valley dark solitons from the defocusing Hirota equation by the Darboux transformation.Particularly,we report a remarkable phenomenon for the inelastic interaction of the double-valley dark solitons,in contrast to the solitons interacting with each other elastically for a scalar integrable model in previous works.Furthermore,we give the explicit conditions for the elastic collision based on the asymptotic analysis results.It is shown that the double-valley dark solitons could also admit elastic interaction under the special parameters settings.展开更多
Interactions between plants and insects are among the most important life functions for all organism at a particular natural community.Usually a large number of samples are required to identify insect diets in food we...Interactions between plants and insects are among the most important life functions for all organism at a particular natural community.Usually a large number of samples are required to identify insect diets in food web studies.Previously,Sanger sequencing and next generation sequencing(NGS)with short DNA barcodes were used,resulting in low species-level identification;meanwhile the costs of Sanger sequencing are expensive for metabarcoding together with more samples.Here,we present a fast and effective sequencing strategy to identify larvae of Lepidoptera and their diets at the same time without increasing the cost on Illumina platform in a single HiSeq run,with long-multiplexmetabarcoding(COI for insects,rbcL,matK,ITS and trnL for plants)obtained by Trinity assembly(SHMMT).Meanwhile,Sanger sequencing(for single individuals)and NGS(for polyphagous)were used to verify the reliability of the SHMMT approach.Furthermore,we show that SHMMT approach is fast and reliable,with most high-quality sequences of five DNA barcodes of 63 larvae individuals(54 species)recovered(full length of 100%of the COI gene and 98.3%of plant DNA barcodes)using Trinity assembly(up-sized to 1015 bp).For larvae diets identification,95%are reliable;the other 5%failed because their guts were empty.The diets identified by SHMMT approach are 100%consistent with the host plants that the larvae were feeding on during our collection.Our study demonstrates that SHMMT approach is reliable and cost-effective for insect-plants network studies.This will facilitate insect-host plant studies that generally contain a huge number of samples.展开更多
基金Supported by the Military Medical Science and Technology Committee of China,No.14MS095the Quanzhou Science and Technology Planning Project,No.2017Z018.
文摘BACKGROUND Microwave ablation(MWA)is an effective treatment option for patients with primary liver cancer.However,it has been reported that the MWA procedure induces a hepatic inflammatory response and injury,which may negatively affect the efficacy of MWA.As such,the discovery of reliable markers to monitor the patient’s response to MWA is needed.Golgi protein 73(GP73)has been shown to be associated with chronic liver disease.To date,the potential value of serum GP73 in the dynamic monitoring during MWA of liver cancer remains unclear.AIM To examine the effects of MWA on the serum levels of GP73 in patients with primary liver cancer.METHODS A total of 150 primary liver cancer patients with a single small lesion(≤3 cm in diameter)were retrospectively enrolled spanning the period between January 2016 and October 2018.All of the patients received MWA for the treatment of primary liver cancer.Serum GP73,alpha-fetoprotein(AFP),and widely used liver biochemical indicators[serum albumin,total bilirubin(TBIL),alanine aminotransferase(ALT),and aspartate aminotransferase(AST)]were compared before MWA and at different time points,including 1,2,and 4 wk following the ablation procedure.RESULTS Complete tumor ablation was achieved in 95.33%of the patients at 1 mo after MWA.The 1-,2-,and 3-year disease-free survival rates were 74.67%,59.33%,and 54.00%,respectively.The serum AFP levels were significantly decreased at 1,2,and 4 wk after MWA;they returned to the normal range at 12 wk after MWA;and they remained stable thereafter during follow-up in those cases without recurrence.In contrast,the serum GP73 levels were significantly increased at 1 and 2 wk after MWA.The serum GP73 levels reached the peak at 2 wk after MWA,started to decline after hepatoprotective treatment with glycyrrhizin and reduced glutathione,and returned to the pretreatment levels at 12 and 24 wk after MWA.Notably,the changes of serum GP73 in response to MWA were similar to those of TBIL,ALT,and AST.CONCLUSION Serum GP73 is markedly increased in response to MWA of liver cancer.Thus,serum GP73 holds potential as a marker to monitor MWA-induced inflammatory liver injury in need of amelioration.
基金This project was supported by the Xuzhou Science and Technology Plan Project(No.KC19223)Natural Science Project of Xuzhou Medical University(No.2018KJ07).
文摘Objective:Pain is a prevalent and distressing symptom in patients with cancer which has a significant impact on patients’physical health,psychological well-being,and quality of life(QOL).Deficiency of pain care,inadequate pain communication,and reluctance toward pain medication contribute to the difficulties in improving the situation of patients with cancer pain.Fortunately,the integration of self-management and professional healthcare provides new opportunities for patients with cancer pain.Methods:A two-arm randomized controlled trial will be conducted to assess the effectiveness of self-management application compared with routine care among patients with cancer pain.Based on Orem’s self-care theory and Naylor’s transitional care theory,cancer pain self-management application“Pain-housekeeper”was developed with dual client:patient client and medical client.A total of 120 patients with cancer pain are recruited from two university-affiliated tertiary public hospitals in China.They are randomly assigned to the control group and the intervention group.The patients in the control group get routine care while the patients in the intervention group get routine care and Pain-housekeeper App.The intervention is delivered by registered doctors,nurses,and postgraduate nursing students specialized in pain care.Effect measurements for both groups are carried out with questionnaires at baseline(T0),after 4 weeks(T1)and after 12 weeks(T2).The primary outcomes include pain condition,self-efficacy,and QOL.The secondary outcomes include pain medication adherence,satisfaction,anxiety,and depression.Compliance of data submission,economic evaluation,usage,and clinical data are also considered in the test.Data for the evaluation are gathered continuously during the study period.Discussion:This article provides insight into the effectiveness of a theory-based self-management mobile application intervention delivered to patients with uncontrolled cancer pain.The results are used to help patients to improve cancer pain control,self-efficacy,and QOL.Conclusions:The findings of this article provide evidence to support the implementation of an innovative and easily accessible intervention that enhances health outcomes.
基金Supported by the National Natural Science Foundation of China(Grant Nos.11771151,12022513,11775176)the Guangzhou Science and Technology Program(Grant No.201904010362)+1 种基金the Fundamental Research Funds for Central Universities(Grant No.2019MS110)the Major Basic Research Program of Natural Science of Shaanxi Province(Grant No.2018KJXX-094)。
文摘For a scalar integrable model,it is generally believed that the solitons interact with each other elastically,for instance,multi-bright solitons from the nonlinear Schrodinger equation and the Korteweg-de Vries equation,etc.We obtain double-valley dark solitons from the defocusing Hirota equation by the Darboux transformation.Particularly,we report a remarkable phenomenon for the inelastic interaction of the double-valley dark solitons,in contrast to the solitons interacting with each other elastically for a scalar integrable model in previous works.Furthermore,we give the explicit conditions for the elastic collision based on the asymptotic analysis results.It is shown that the double-valley dark solitons could also admit elastic interaction under the special parameters settings.
基金by the China National Funds for Distinguished Young Scien・tists(to Zhang,Grant No.31425023)Natural Science Foundation of China(to Zhang,Grant 31772501)+2 种基金Support Project of High-level Teachers in Beijing Municipal Universities(No.IDHT20180518)Academy for Multidisciplinary StudiesCapital Normal University and also supported by Program for Changjiang Scholars and Innovative Research Team in University(IRT_17R75).
文摘Interactions between plants and insects are among the most important life functions for all organism at a particular natural community.Usually a large number of samples are required to identify insect diets in food web studies.Previously,Sanger sequencing and next generation sequencing(NGS)with short DNA barcodes were used,resulting in low species-level identification;meanwhile the costs of Sanger sequencing are expensive for metabarcoding together with more samples.Here,we present a fast and effective sequencing strategy to identify larvae of Lepidoptera and their diets at the same time without increasing the cost on Illumina platform in a single HiSeq run,with long-multiplexmetabarcoding(COI for insects,rbcL,matK,ITS and trnL for plants)obtained by Trinity assembly(SHMMT).Meanwhile,Sanger sequencing(for single individuals)and NGS(for polyphagous)were used to verify the reliability of the SHMMT approach.Furthermore,we show that SHMMT approach is fast and reliable,with most high-quality sequences of five DNA barcodes of 63 larvae individuals(54 species)recovered(full length of 100%of the COI gene and 98.3%of plant DNA barcodes)using Trinity assembly(up-sized to 1015 bp).For larvae diets identification,95%are reliable;the other 5%failed because their guts were empty.The diets identified by SHMMT approach are 100%consistent with the host plants that the larvae were feeding on during our collection.Our study demonstrates that SHMMT approach is reliable and cost-effective for insect-plants network studies.This will facilitate insect-host plant studies that generally contain a huge number of samples.
