AIM: To study the role of immature dendritic cells (imDCs) on immune tolerance in rat penetrating keratoplasty (PKP) in high -risk eyes and to investigate the mechanism of immune hyporesponsiveness induced by donor-de...AIM: To study the role of immature dendritic cells (imDCs) on immune tolerance in rat penetrating keratoplasty (PKP) in high -risk eyes and to investigate the mechanism of immune hyporesponsiveness induced by donor-derived imDCs. ·METHODS: Seventy-five SD rats (recipient) and 39 Wistar rats (donor) were randomly divided into 3 groups: control, imDC and mature dendritic cell (mDC) group respectively. Using a model of orthotopic corneal transplantation in which allografts were placed in neovascularized high -risk eyes of recipient rat. Corneal neovascularization was induced by alkaline burn in the central cornea of recipient rat. Recipients in imDC group or mDC group were injected donor bone marrow-derived imDCs or mDCs of 1 ×10 6 respectively 1 week before corneal transplantation via tail vein. Control rat received the same volume of PBS. In each group, 16 recipients were kept for determination of survival time and other 9 recipients were executed on day 3, 7 and 14 after transplantation. Cornea was harvested for hematoxylin - eosin staining and acute rejection evaluation, Western blot was used to detect the expression level of Foxp3. ·RESULTS: The mean survival time of imDC group was significantly longer than that of control and mDC groups (all P <0.05). The expression level of Foxp3 on CD4 + CD25 + T cells of imDC group (2.24 ±0.18) was significantly higher than that in the control (1.68 ±0.09) and mDC groups (1.46±0.13) (all P <0.05).·CONCLUSION: Donor -derived imDC is an effective treatment in inducing immune hyporesponsiveness in rat PKP. The mechanism of immune tolerance induced by imDC might be inhibit T lymphocytes responsiveness by regulatory T cells.展开更多
AIM:To evaluate the efficacy,safety and stability of posterior chamber phakic intraocular lens implantation for the correction of high myopia.METHODS:Retrospective case review of 82 eyes(43patients)undergoing implanta...AIM:To evaluate the efficacy,safety and stability of posterior chamber phakic intraocular lens implantation for the correction of high myopia.METHODS:Retrospective case review of 82 eyes(43patients)undergoing implantable Coliamer lens(ICL)placement by a single surgeon(Xiao-Wei Gao)to correct preoperative mean spherical equivalents between-9.00diopter(D)and-23.00D.Main outcome measures included uncorrected visual acuity(UCVA),refraction,best spectacle-corrected visual acuity(BSCVA),endothelial cell density(ECD),intraocular pressure(IOP),lens transparency,postoperative uveitis.Visante anterior segment optical coherence tomography(AS-OCT)was used to measure anterior chamber depth(ACD)and the position of ICL.RESULTS:Mean follow-up was 6.54±3.26 months(range 3-12 months).Predictability of the manifest spherical equivalent(SE)refraction to within±1.OOD was achieved in 88%of eyes and±0.50D in 72.5%of eyes.The mean postoperative manifest SE refraction was-1.85±0.72D,with 96.34%of eyes maintaining or gaining≥1 line(s)of BSCVA.The mean 3-month postoperative ECD decreased but had no statistically difference compared with the preoperative ECD.Of the 7 eyes(8.54%)with a mild transient increase in intraocular pressure(up to 30mmHg),none required a second surgical procedure or prolonged topical medication.There was no loss of lens transparency.Pigmented precipitates were observed in 5 eyes(6.09%).The mean preoperative ACD measured with AS-OCT was 3.28±0.14mm,three months after surgery,the mean ACD was2.45±0.22mm.Anterior chamber depth showed astatistically significant reduction.One eye(1.22%)had ICL spontaneous rotation,81 eyes(98.78%)of the lens remained correctly centered.CONCLUSION:The implantation of ICL is an effective surgical option for the management of high myopia.But its long time effect and safety still need more time to prove.展开更多
AIM: To demonstrate the changes in ultrastructure and histopathology of the cornea in acute corneal alkaline burns after femtosecond laser-assisted deep lamellar keratoplasty.·METHODS: The New Zealand white rabbi...AIM: To demonstrate the changes in ultrastructure and histopathology of the cornea in acute corneal alkaline burns after femtosecond laser-assisted deep lamellar keratoplasty.·METHODS: The New Zealand white rabbits treated with alkaline corneal burn were randomized into two groups,Group A(16 eyes) with femtosecond laser-assisted deep lamellar keratoplasty 24 h after burn and Group B(16 eyes)without keratoplasty as controls. All eyes were evaluated with transmission electron microscopy(TEM) at 1, 2, 3,and 4wk follow-up, then all corneas were tested by hematoxylin and eosin staining histology.· RESULTS: The corneal grafts in Group A were transparent, while those in Group B showed corneal stromal edema and loosely arranged collagen fibers. One week after treatment, TEM revealed the intercellular desmosomes in the epithelial layers and intact non-dissolving nuclei in Group A. At week 4, the center of the corneas in Group A was transparent with regularly arranged collagen fibers and fibroblasts in the stroma. In Group B, squamous cells were observed on the corneal surface and some epithelial cells were detached.· CONCLUSION: Femtosecond laser-assisted deep lamellar keratoplasty can suppress inflammatory responses, prevent toxic substance-induced injury to the corneal endothelium and inner tissues with quicker recovery and better visual outcomes.展开更多
·AIM:To assess features of cavernous hemangioma (CH) in the orbit revealed by CT and MRI and summarize prediction of preoperative CT and MRI for the adhesion degree of CH in the orbit.·METHODS:A total of 97 ...·AIM:To assess features of cavernous hemangioma (CH) in the orbit revealed by CT and MRI and summarize prediction of preoperative CT and MRI for the adhesion degree of CH in the orbit.·METHODS:A total of 97 patients with pathologically confirmed CH in the orbit were examined with axial and coronal CT scan,and axial,coronal,sagittal,and enhanced fat suppression MRI scan.CT and MRI findings and intraoperative adhesion degrees were retrospectively analyzed.·RESULTS:There were 47 patients with slight adhesion,for whom CT and MRI showed round masses with well defined margins in the extraocular muscles;14 patients with mild adhesion,for whom CT and MRI revealed irregular masses with unclear boundary between CH and the optic nerve in coronal images,and emissary veins in the posterior region of masses in contrast-enhanced images;36 patients with severe adhesion,for whom CT and MRI exhibited an irregular or ovoid mass filling the orbital apex,or showed distorted and even spiky margins in the posterior region of masses in contrast enhanced images at the presence of a transparent triangle between the mass and the orbital apex.·CONCLUSION:Preoperative CT and MRI aid in accurate diagnosis,selection of the surgical approach,and assessment of the adhesion degree and surgical risks for CH.·展开更多
AIM: To explore the possibility of human umbilical cord mesenchymal stem cells(h UCMSCs), human umbilical vein endothelial cells(h UVECs), human dental pulp stem cells(h DPSCs) and human periodontal ligament stem cell...AIM: To explore the possibility of human umbilical cord mesenchymal stem cells(h UCMSCs), human umbilical vein endothelial cells(h UVECs), human dental pulp stem cells(h DPSCs) and human periodontal ligament stem cells(h PDLSCs) serving as feeder cells in co-culture systems for the cultivation of limbal stem cells.METHODS: Different feeder layers were cultured in Dulbecco's modified Eagle's medium(DMEM)/F12 and were treated with mitomycin C. Rabbits limbal stem cells(LSCs) were co-cultured on h UCMSCs, h UVECs, h DPSCs, h PDLSCs and NIH-3T3, and then comparative analysis were made between each group to see their respective colony-forming efficiency(CFE) assay and immunofluorescence(IPO13,CK3/12).RESULTS: The efficiency of the four type cells in supporting the LSCs morphology and its cellular differentiation was similar to that of NIH-3T3 fibroblasts as demonstrated by the immunostaining properties analysis, with each group exhibiting a similar strong expression pattern of IPO13, but lacking CK3 and CK12 expression in terms of immunostaining. But h UCMSCs, h DPSCs and h PDLSCs feeder layers were superior in promoting colony formation potential of cells when compared to h UVECs and feedercell-free culture.CONCLUSION: hUCMSCs, hDPSCs and hPDLSCs can be a suitable alternative to conventional mouse NIH-3T3 feeder cells, so that risk of zoonotic infection can be diminished.展开更多
·AIM:To study the effect of theminocycline (MNC) on expression of bcl-2,bax in retinal nerve cells of rat with diabetes.·METHODS:Male SD rats were randomly divided into negative control group,model control g...·AIM:To study the effect of theminocycline (MNC) on expression of bcl-2,bax in retinal nerve cells of rat with diabetes.·METHODS:Male SD rats were randomly divided into negative control group,model control group and MNC treated group.Diabetes model was established by intraperitoneal injection of 60mg/kg streptozotocin (STZ).The protein expressions of bcl-2 and bax in retina were detected by immunohistochemistry method.·RESULTS:Compared with the negative control group,bax immunoreactive neurons in retina were increased significantly (P<0.01) in model control group.However,bax immun-oreactive neurons in retina in MNC treated group were significantly decreased (P<0.01).Compared with the model control group,bcl-2 immunoreactive neurons in retina were increased significantly(P<0.01) in MNC treated group.·CONCLUSION:MNC can obviously decreased expression of bax and increased expression of bcl-2 in retina with DR.It is one of path of inhibiting impairment on retinal nerve cells with DR.·展开更多
This paper presents a new strategy of using the radial integration boundary element method (RIBEM) to solve non-homogeneous heat conduction and thermoelasticity problems. In the method, the evaluation of the radial in...This paper presents a new strategy of using the radial integration boundary element method (RIBEM) to solve non-homogeneous heat conduction and thermoelasticity problems. In the method, the evaluation of the radial in-tegral which is used to transform domain integrals to equivalent boundary integrals is carried out on the basis of elemental nodes. As a result, the computational time spent in evaluating domain integrals can be saved considerably in comparison with the conventional RIBEM. Three numerical examples are given to demonstrate the correctness and computational efficiency of the proposed approach.展开更多
AIM:To explore the protective effects of amino-guanidine(AG) on retinal apoptosis in mice with oxygeninduced retinopathy(OIR).·METHODS:A total of 80 C57BL/6J mice,aged 7 days,were randomly divided into four group...AIM:To explore the protective effects of amino-guanidine(AG) on retinal apoptosis in mice with oxygeninduced retinopathy(OIR).·METHODS:A total of 80 C57BL/6J mice,aged 7 days,were randomly divided into four groups:normal,high oxygen,high oxygen saline and high oxygen treated with AG.In the normal group,mice were housed in normoxic conditions from postnatal day P7 to P17.Mice in the other 3 groups were placed under hyperoxic conditions(75 ±2% O2) in an oxygen-regulated chamber for 5 days and subsequently placed in normoxic conditions for 5days.Mice in the AG group were treated once daily,from P12 to P17,with AG hemisulfate(100mg/kg body weight,intraperitoneally) dissolved in physiological saline.An equivalent amount of 0.9% physiological saline was administered,as above,to mice in the high oxygen saline group.Ten mice were randomly selected from each group on P14 and on P17,euthanized and the retinas examined.Apoptotic cells in the retina were detected using the terminal-deoxynucleoitidyl transferase mediated nick end labeling(TUNEL) method.The expression of nitric oxide synthase(iNOS) in the retina was detected by immunohistochemistry and changes in rod cells were observed using electron microscopy.·RESULTS:TUNEL-positive cells and iNOS immunoreactive neurons were present in the inner nuclear and ganglion cell retinal layers of mice in the high oxygen group.The number of TUNEL-positive cells was significantly greater in the high oxygen group compared with the normal group(t =-20.81,P14d<0.05;t =-15.05,P17d<0.05).However,the number of TUNEL-positive cells in the AG treatment group was significantly lower(t =-13.21,P14d<0.05;t =-6.61,P17d<0.05) compared with thehigh oxygen group.The expression of iNOS was significantly higher in the high oxygen group compared with the normal group(t =-21.95,P14d<0.05;t =-17.30,P17d<0.05).However,the expression of iNOS in the AG treatment group was significantly lower(t =-12.17,P14d<0.05;t =-10.30,P17d<0.05) compared with the high oxygen group.The outer segments of the rods were disorganized and short in the high oxygen group.Rod morphology appeared to be slightly improved in the AG group.·CONCLUSION:AG may protect retinal neurons in OIR by inhibiting apoptosis.The mechanism may be related to iNOS.展开更多
AIM:To investigate the effect of intravitreal injection administered sorafenib,a multikinase inhibitor,in a rat model of oxygen-induced retinopathy(OIR).METHODS:Seven-day-old Sprague-Dawley rats(n=144)were randomly as...AIM:To investigate the effect of intravitreal injection administered sorafenib,a multikinase inhibitor,in a rat model of oxygen-induced retinopathy(OIR).METHODS:Seven-day-old Sprague-Dawley rats(n=144)were randomly assigned to six groups.Group A received normal partial oxygen pressure and groups B,C,D,E and F were exposed to hyperoxia(75±2)%from postnatal 7d(P7)to P12 to induce retinopathy of prematurity.The rats in groups C,D,E and F were received intravitreal injections of either vehicle(DMSO)or sorafenib at P12(5,20 and 80μg,respectively).Then they returned to normoxia after P12.The retinas were whole-mounted and imaged with a confocal microscopy.The vascular branching points were counted to quantify neovascularization at P17.Cross-sections of the retina were stained with hematoxylin and eosin(HE).The nuclei of new vessels breaking the internal limiting membrane were counted to quantify the proliferative neovascular response.RESULTS:The retinal vessel in groups B and C turned into tortuosity and a great deal of neovascularization were observed.Sorafenib-treated rats had significantly less neovascularization as compared with vehicle-treated and control rats in a dose dependent manner(P<0.05).The number of vascular branching points in A,B,C,D,E and F were 16.50±3.90,37.44±6.47,37.08±5.10,30.80±6.85,26.08±5.08 and 19.83±3.51,respectively.The number of the nuclei of retinal new vessel in A,B,C,D,E and F were 0.22±0.42,35.66±4.70,35.30±4.54,27.30±4.28,21.41±3.53,and 7.41±2.87,respectively.There were significant difference between each group(P<0.05)except groups B and C.CONCLUSION:In the rat OIR model,sorafenib could inhibit retinal neovascularization in a dose dependent manner.展开更多
AIM:To compare the clinical effects of the modified and conventional secondary hydroxyapatite orbital implantations.METHODS:A total of 40 patients who had received eye enucleation were equally randomized into the modi...AIM:To compare the clinical effects of the modified and conventional secondary hydroxyapatite orbital implantations.METHODS:A total of 40 patients who had received eye enucleation were equally randomized into the modified and conventional groups.Twenty patients were treated by conventional method.The four rectus muscles were separated,and then an orbital implant wrapped with xenogenous sclera was implanted.Twenty patients were treated by modified method.An implant unwrapped with xenogenous sclera was directly implanted into the muscle pyramid.The operating time,costs,clinical effects,and complications of the two groups were compared.RESULTS:The average operating time of the modified group was 20.5±5.6min,whereas that of the conventional group was 56.8±14.6min(P<0.01).The average cost of the modified group was 7 800±340RMB(1 274±55.6USD),whereas that of the conventional group was 9 800±660RMB(1 601±107.8USD)(P<0.01).The two groups did not show significant difference in orbital implant mobility or postoperative complications.CONCLUSION:The modified secondary hydroxyapatite orbital implantation has advantages in operating time,surgery cost,and complication reducing.It is worthy for wide clinical application and further study.展开更多
AIM:To investigate the mechanism and effect of trabecular tissue repair for corneal defect,and to provide a theoretical basis for its clinical application.METHODS:Trabeculectomy was performed on 40(80 eyes)of 70 New Z...AIM:To investigate the mechanism and effect of trabecular tissue repair for corneal defect,and to provide a theoretical basis for its clinical application.METHODS:Trabeculectomy was performed on 40(80 eyes)of 70 New Zealand white rabbits.Take trabecular tissue for backup.Thirty(30 eyes)corneal defect models were made,trabecular tissue was filled in the corneal defect,and the oblique cross stitch was used to suture the corneal laceration and debridement.Anterior segment image and optical coherence tomography(OCT)were performed at the time 1 d,1 wk,1 and 3 mo after the model was made.After the observation,the cornea was to ken and stained with trypanosome blue-alizarin red and the pathological tissue was examined.RESULTS:Observation 1 wk after surgery,the area of corneal defect was edema,but the corneal curvature was basically normal,and the anterior chamber existed under slit lamp.After 3 mo of observation,most corneal defects were repaired in the form of corneal leucoma and corneal macula(73.3%),the filled trabecular tissue gradually became transparent,fused tightly with the corneal tissue,and the corneal curvature was relatively smooth.But in one case,the trabecular planter was partially detached,no serious complications such as corneal laceration occurred after the stitches were removed.CONCLUSION:The trabecular tissue structure is similar to the corneal,and it can be used as a substitute for the corneal tissue defect by providing fiber scaffolds and cell amplification differentiation,and lay a foundation for the second-stage surgical treatment.展开更多
基金Military Medical Research Projects Fund of China (No. CLZ11JA25)
文摘AIM: To study the role of immature dendritic cells (imDCs) on immune tolerance in rat penetrating keratoplasty (PKP) in high -risk eyes and to investigate the mechanism of immune hyporesponsiveness induced by donor-derived imDCs. ·METHODS: Seventy-five SD rats (recipient) and 39 Wistar rats (donor) were randomly divided into 3 groups: control, imDC and mature dendritic cell (mDC) group respectively. Using a model of orthotopic corneal transplantation in which allografts were placed in neovascularized high -risk eyes of recipient rat. Corneal neovascularization was induced by alkaline burn in the central cornea of recipient rat. Recipients in imDC group or mDC group were injected donor bone marrow-derived imDCs or mDCs of 1 ×10 6 respectively 1 week before corneal transplantation via tail vein. Control rat received the same volume of PBS. In each group, 16 recipients were kept for determination of survival time and other 9 recipients were executed on day 3, 7 and 14 after transplantation. Cornea was harvested for hematoxylin - eosin staining and acute rejection evaluation, Western blot was used to detect the expression level of Foxp3. ·RESULTS: The mean survival time of imDC group was significantly longer than that of control and mDC groups (all P <0.05). The expression level of Foxp3 on CD4 + CD25 + T cells of imDC group (2.24 ±0.18) was significantly higher than that in the control (1.68 ±0.09) and mDC groups (1.46±0.13) (all P <0.05).·CONCLUSION: Donor -derived imDC is an effective treatment in inducing immune hyporesponsiveness in rat PKP. The mechanism of immune tolerance induced by imDC might be inhibit T lymphocytes responsiveness by regulatory T cells.
文摘AIM:To evaluate the efficacy,safety and stability of posterior chamber phakic intraocular lens implantation for the correction of high myopia.METHODS:Retrospective case review of 82 eyes(43patients)undergoing implantable Coliamer lens(ICL)placement by a single surgeon(Xiao-Wei Gao)to correct preoperative mean spherical equivalents between-9.00diopter(D)and-23.00D.Main outcome measures included uncorrected visual acuity(UCVA),refraction,best spectacle-corrected visual acuity(BSCVA),endothelial cell density(ECD),intraocular pressure(IOP),lens transparency,postoperative uveitis.Visante anterior segment optical coherence tomography(AS-OCT)was used to measure anterior chamber depth(ACD)and the position of ICL.RESULTS:Mean follow-up was 6.54±3.26 months(range 3-12 months).Predictability of the manifest spherical equivalent(SE)refraction to within±1.OOD was achieved in 88%of eyes and±0.50D in 72.5%of eyes.The mean postoperative manifest SE refraction was-1.85±0.72D,with 96.34%of eyes maintaining or gaining≥1 line(s)of BSCVA.The mean 3-month postoperative ECD decreased but had no statistically difference compared with the preoperative ECD.Of the 7 eyes(8.54%)with a mild transient increase in intraocular pressure(up to 30mmHg),none required a second surgical procedure or prolonged topical medication.There was no loss of lens transparency.Pigmented precipitates were observed in 5 eyes(6.09%).The mean preoperative ACD measured with AS-OCT was 3.28±0.14mm,three months after surgery,the mean ACD was2.45±0.22mm.Anterior chamber depth showed astatistically significant reduction.One eye(1.22%)had ICL spontaneous rotation,81 eyes(98.78%)of the lens remained correctly centered.CONCLUSION:The implantation of ICL is an effective surgical option for the management of high myopia.But its long time effect and safety still need more time to prove.
基金Supported by the Military Medical Science and Technology General Project During the 12thFive-Year Plan Period(No.CWS11J239)Autonomous Region of Xinjiang the Mandatory Project of Science and Technology(No.201491171)
文摘AIM: To demonstrate the changes in ultrastructure and histopathology of the cornea in acute corneal alkaline burns after femtosecond laser-assisted deep lamellar keratoplasty.·METHODS: The New Zealand white rabbits treated with alkaline corneal burn were randomized into two groups,Group A(16 eyes) with femtosecond laser-assisted deep lamellar keratoplasty 24 h after burn and Group B(16 eyes)without keratoplasty as controls. All eyes were evaluated with transmission electron microscopy(TEM) at 1, 2, 3,and 4wk follow-up, then all corneas were tested by hematoxylin and eosin staining histology.· RESULTS: The corneal grafts in Group A were transparent, while those in Group B showed corneal stromal edema and loosely arranged collagen fibers. One week after treatment, TEM revealed the intercellular desmosomes in the epithelial layers and intact non-dissolving nuclei in Group A. At week 4, the center of the corneas in Group A was transparent with regularly arranged collagen fibers and fibroblasts in the stroma. In Group B, squamous cells were observed on the corneal surface and some epithelial cells were detached.· CONCLUSION: Femtosecond laser-assisted deep lamellar keratoplasty can suppress inflammatory responses, prevent toxic substance-induced injury to the corneal endothelium and inner tissues with quicker recovery and better visual outcomes.
文摘·AIM:To assess features of cavernous hemangioma (CH) in the orbit revealed by CT and MRI and summarize prediction of preoperative CT and MRI for the adhesion degree of CH in the orbit.·METHODS:A total of 97 patients with pathologically confirmed CH in the orbit were examined with axial and coronal CT scan,and axial,coronal,sagittal,and enhanced fat suppression MRI scan.CT and MRI findings and intraoperative adhesion degrees were retrospectively analyzed.·RESULTS:There were 47 patients with slight adhesion,for whom CT and MRI showed round masses with well defined margins in the extraocular muscles;14 patients with mild adhesion,for whom CT and MRI revealed irregular masses with unclear boundary between CH and the optic nerve in coronal images,and emissary veins in the posterior region of masses in contrast-enhanced images;36 patients with severe adhesion,for whom CT and MRI exhibited an irregular or ovoid mass filling the orbital apex,or showed distorted and even spiky margins in the posterior region of masses in contrast enhanced images at the presence of a transparent triangle between the mass and the orbital apex.·CONCLUSION:Preoperative CT and MRI aid in accurate diagnosis,selection of the surgical approach,and assessment of the adhesion degree and surgical risks for CH.·
基金Supported by the Project Plan of Science and Technology Assistance in Xinjiang Autonomous Region(No.201491171)
文摘AIM: To explore the possibility of human umbilical cord mesenchymal stem cells(h UCMSCs), human umbilical vein endothelial cells(h UVECs), human dental pulp stem cells(h DPSCs) and human periodontal ligament stem cells(h PDLSCs) serving as feeder cells in co-culture systems for the cultivation of limbal stem cells.METHODS: Different feeder layers were cultured in Dulbecco's modified Eagle's medium(DMEM)/F12 and were treated with mitomycin C. Rabbits limbal stem cells(LSCs) were co-cultured on h UCMSCs, h UVECs, h DPSCs, h PDLSCs and NIH-3T3, and then comparative analysis were made between each group to see their respective colony-forming efficiency(CFE) assay and immunofluorescence(IPO13,CK3/12).RESULTS: The efficiency of the four type cells in supporting the LSCs morphology and its cellular differentiation was similar to that of NIH-3T3 fibroblasts as demonstrated by the immunostaining properties analysis, with each group exhibiting a similar strong expression pattern of IPO13, but lacking CK3 and CK12 expression in terms of immunostaining. But h UCMSCs, h DPSCs and h PDLSCs feeder layers were superior in promoting colony formation potential of cells when compared to h UVECs and feedercell-free culture.CONCLUSION: hUCMSCs, hDPSCs and hPDLSCs can be a suitable alternative to conventional mouse NIH-3T3 feeder cells, so that risk of zoonotic infection can be diminished.
文摘·AIM:To study the effect of theminocycline (MNC) on expression of bcl-2,bax in retinal nerve cells of rat with diabetes.·METHODS:Male SD rats were randomly divided into negative control group,model control group and MNC treated group.Diabetes model was established by intraperitoneal injection of 60mg/kg streptozotocin (STZ).The protein expressions of bcl-2 and bax in retina were detected by immunohistochemistry method.·RESULTS:Compared with the negative control group,bax immunoreactive neurons in retina were increased significantly (P<0.01) in model control group.However,bax immun-oreactive neurons in retina in MNC treated group were significantly decreased (P<0.01).Compared with the model control group,bcl-2 immunoreactive neurons in retina were increased significantly(P<0.01) in MNC treated group.·CONCLUSION:MNC can obviously decreased expression of bax and increased expression of bcl-2 in retina with DR.It is one of path of inhibiting impairment on retinal nerve cells with DR.·
基金supported by the National Natural Science Foundation of China (10872050, 11172055)the Fundamental Research Funds for the Centred Universities (DUT11ZD(G)01)
文摘This paper presents a new strategy of using the radial integration boundary element method (RIBEM) to solve non-homogeneous heat conduction and thermoelasticity problems. In the method, the evaluation of the radial in-tegral which is used to transform domain integrals to equivalent boundary integrals is carried out on the basis of elemental nodes. As a result, the computational time spent in evaluating domain integrals can be saved considerably in comparison with the conventional RIBEM. Three numerical examples are given to demonstrate the correctness and computational efficiency of the proposed approach.
文摘AIM:To explore the protective effects of amino-guanidine(AG) on retinal apoptosis in mice with oxygeninduced retinopathy(OIR).·METHODS:A total of 80 C57BL/6J mice,aged 7 days,were randomly divided into four groups:normal,high oxygen,high oxygen saline and high oxygen treated with AG.In the normal group,mice were housed in normoxic conditions from postnatal day P7 to P17.Mice in the other 3 groups were placed under hyperoxic conditions(75 ±2% O2) in an oxygen-regulated chamber for 5 days and subsequently placed in normoxic conditions for 5days.Mice in the AG group were treated once daily,from P12 to P17,with AG hemisulfate(100mg/kg body weight,intraperitoneally) dissolved in physiological saline.An equivalent amount of 0.9% physiological saline was administered,as above,to mice in the high oxygen saline group.Ten mice were randomly selected from each group on P14 and on P17,euthanized and the retinas examined.Apoptotic cells in the retina were detected using the terminal-deoxynucleoitidyl transferase mediated nick end labeling(TUNEL) method.The expression of nitric oxide synthase(iNOS) in the retina was detected by immunohistochemistry and changes in rod cells were observed using electron microscopy.·RESULTS:TUNEL-positive cells and iNOS immunoreactive neurons were present in the inner nuclear and ganglion cell retinal layers of mice in the high oxygen group.The number of TUNEL-positive cells was significantly greater in the high oxygen group compared with the normal group(t =-20.81,P14d<0.05;t =-15.05,P17d<0.05).However,the number of TUNEL-positive cells in the AG treatment group was significantly lower(t =-13.21,P14d<0.05;t =-6.61,P17d<0.05) compared with thehigh oxygen group.The expression of iNOS was significantly higher in the high oxygen group compared with the normal group(t =-21.95,P14d<0.05;t =-17.30,P17d<0.05).However,the expression of iNOS in the AG treatment group was significantly lower(t =-12.17,P14d<0.05;t =-10.30,P17d<0.05) compared with the high oxygen group.The outer segments of the rods were disorganized and short in the high oxygen group.Rod morphology appeared to be slightly improved in the AG group.·CONCLUSION:AG may protect retinal neurons in OIR by inhibiting apoptosis.The mechanism may be related to iNOS.
文摘AIM:To investigate the effect of intravitreal injection administered sorafenib,a multikinase inhibitor,in a rat model of oxygen-induced retinopathy(OIR).METHODS:Seven-day-old Sprague-Dawley rats(n=144)were randomly assigned to six groups.Group A received normal partial oxygen pressure and groups B,C,D,E and F were exposed to hyperoxia(75±2)%from postnatal 7d(P7)to P12 to induce retinopathy of prematurity.The rats in groups C,D,E and F were received intravitreal injections of either vehicle(DMSO)or sorafenib at P12(5,20 and 80μg,respectively).Then they returned to normoxia after P12.The retinas were whole-mounted and imaged with a confocal microscopy.The vascular branching points were counted to quantify neovascularization at P17.Cross-sections of the retina were stained with hematoxylin and eosin(HE).The nuclei of new vessels breaking the internal limiting membrane were counted to quantify the proliferative neovascular response.RESULTS:The retinal vessel in groups B and C turned into tortuosity and a great deal of neovascularization were observed.Sorafenib-treated rats had significantly less neovascularization as compared with vehicle-treated and control rats in a dose dependent manner(P<0.05).The number of vascular branching points in A,B,C,D,E and F were 16.50±3.90,37.44±6.47,37.08±5.10,30.80±6.85,26.08±5.08 and 19.83±3.51,respectively.The number of the nuclei of retinal new vessel in A,B,C,D,E and F were 0.22±0.42,35.66±4.70,35.30±4.54,27.30±4.28,21.41±3.53,and 7.41±2.87,respectively.There were significant difference between each group(P<0.05)except groups B and C.CONCLUSION:In the rat OIR model,sorafenib could inhibit retinal neovascularization in a dose dependent manner.
文摘AIM:To compare the clinical effects of the modified and conventional secondary hydroxyapatite orbital implantations.METHODS:A total of 40 patients who had received eye enucleation were equally randomized into the modified and conventional groups.Twenty patients were treated by conventional method.The four rectus muscles were separated,and then an orbital implant wrapped with xenogenous sclera was implanted.Twenty patients were treated by modified method.An implant unwrapped with xenogenous sclera was directly implanted into the muscle pyramid.The operating time,costs,clinical effects,and complications of the two groups were compared.RESULTS:The average operating time of the modified group was 20.5±5.6min,whereas that of the conventional group was 56.8±14.6min(P<0.01).The average cost of the modified group was 7 800±340RMB(1 274±55.6USD),whereas that of the conventional group was 9 800±660RMB(1 601±107.8USD)(P<0.01).The two groups did not show significant difference in orbital implant mobility or postoperative complications.CONCLUSION:The modified secondary hydroxyapatite orbital implantation has advantages in operating time,surgery cost,and complication reducing.It is worthy for wide clinical application and further study.
基金Supported by Lanzhou Military Region Scientific Research Fund Project(No.CLZI3JB29)General Hospital of Xinjiang Military Region Key Supported Scientific Research Project(No.2020jzbj1002)。
文摘AIM:To investigate the mechanism and effect of trabecular tissue repair for corneal defect,and to provide a theoretical basis for its clinical application.METHODS:Trabeculectomy was performed on 40(80 eyes)of 70 New Zealand white rabbits.Take trabecular tissue for backup.Thirty(30 eyes)corneal defect models were made,trabecular tissue was filled in the corneal defect,and the oblique cross stitch was used to suture the corneal laceration and debridement.Anterior segment image and optical coherence tomography(OCT)were performed at the time 1 d,1 wk,1 and 3 mo after the model was made.After the observation,the cornea was to ken and stained with trypanosome blue-alizarin red and the pathological tissue was examined.RESULTS:Observation 1 wk after surgery,the area of corneal defect was edema,but the corneal curvature was basically normal,and the anterior chamber existed under slit lamp.After 3 mo of observation,most corneal defects were repaired in the form of corneal leucoma and corneal macula(73.3%),the filled trabecular tissue gradually became transparent,fused tightly with the corneal tissue,and the corneal curvature was relatively smooth.But in one case,the trabecular planter was partially detached,no serious complications such as corneal laceration occurred after the stitches were removed.CONCLUSION:The trabecular tissue structure is similar to the corneal,and it can be used as a substitute for the corneal tissue defect by providing fiber scaffolds and cell amplification differentiation,and lay a foundation for the second-stage surgical treatment.
