BACKGROUND Helicobacter pylori(H.pylori)infection is related to various extragastric diseases including type 2 diabetes mellitus(T2DM).However,the possible mechanisms connecting H.pylori infection and T2DM remain unkn...BACKGROUND Helicobacter pylori(H.pylori)infection is related to various extragastric diseases including type 2 diabetes mellitus(T2DM).However,the possible mechanisms connecting H.pylori infection and T2DM remain unknown.AIM To explore potential molecular connections between H.pylori infection and T2DM.METHODS We extracted gene expression arrays from three online datasets(GSE60427,GSE27411 and GSE115601).Differentially expressed genes(DEGs)commonly present in patients with H.pylori infection and T2DM were identified.Hub genes were validated using human gastric biopsy samples.Correlations between hub genes and immune cell infiltration,miRNAs,and transcription factors(TFs)were further analyzed.RESULTS A total of 67 DEGs were commonly presented in patients with H.pylori infection and T2DM.Five significantly upregulated hub genes,including TLR4,ITGAM,C5AR1,FCER1G,and FCGR2A,were finally identified,all of which are closely related to immune cell infiltration.The gene-miRNA analysis detected 13 miRNAs with at least two gene cross-links.TF-gene interaction networks showed that TLR4 was coregulated by 26 TFs,the largest number of TFs among the 5 hub genes.CONCLUSION We identified five hub genes that may have molecular connections between H.pylori infection and T2DM.This study provides new insights into the pathogenesis of H.pylori-induced onset of T2DM.展开更多
AIM:To quantify the association between Helicobacter pylori(H.pylori)infection and migraine.METHODS:A systematic literature search of PubMed and EMBASE was conducted from inception to December 2013.Studies that provid...AIM:To quantify the association between Helicobacter pylori(H.pylori)infection and migraine.METHODS:A systematic literature search of PubMed and EMBASE was conducted from inception to December 2013.Studies that provided data dealing with H.pylori infection in patients with migraine,as well as healthy controls,were selected.Meta-analysis was carried out using the odds ratio(OR)with a fixed or random effects model,and a 95%CI for the OR was calculated.An unconditional logistic regression model was used to analyze potential parameters related to H.pylori prevalence.Subgroup analyses were conducted as methods of detection and evidence grade.RESULTS:Five case-control studies published between 2000 and 2013 were finally identified,involving 903 patients,with a total H.pylori infection rateof 39.31%.The prevalence of H.pylori infection was significantly greater in migraineurs than in controls(44.97%vs 33.26%,OR=1.92,95%CI:1.05-3.51,P=0.001).A sensitivity test indicated that no single study dominated the combined results.Univariate regression analysis found that publication year,geographical distribution and evidence grade were relevant to the results and were the main reason for the heterogeneity.Subgroup analysis found a significantly greater infection rate of H.pylori in Asian patients with migraine,but no statistically significant infection rate in European patients.The ORs were 3.48(95%CI:2.09-5.81,P=0.000)and 1.19(95%CI:0.86-1.65,P=0.288),respectively.CONCLUSION:The pooled data suggest a trend of more frequent H.pylori infections in patients with migraine.展开更多
基金Supported by National Natural Science Foundation of China,No.82100594.
文摘BACKGROUND Helicobacter pylori(H.pylori)infection is related to various extragastric diseases including type 2 diabetes mellitus(T2DM).However,the possible mechanisms connecting H.pylori infection and T2DM remain unknown.AIM To explore potential molecular connections between H.pylori infection and T2DM.METHODS We extracted gene expression arrays from three online datasets(GSE60427,GSE27411 and GSE115601).Differentially expressed genes(DEGs)commonly present in patients with H.pylori infection and T2DM were identified.Hub genes were validated using human gastric biopsy samples.Correlations between hub genes and immune cell infiltration,miRNAs,and transcription factors(TFs)were further analyzed.RESULTS A total of 67 DEGs were commonly presented in patients with H.pylori infection and T2DM.Five significantly upregulated hub genes,including TLR4,ITGAM,C5AR1,FCER1G,and FCGR2A,were finally identified,all of which are closely related to immune cell infiltration.The gene-miRNA analysis detected 13 miRNAs with at least two gene cross-links.TF-gene interaction networks showed that TLR4 was coregulated by 26 TFs,the largest number of TFs among the 5 hub genes.CONCLUSION We identified five hub genes that may have molecular connections between H.pylori infection and T2DM.This study provides new insights into the pathogenesis of H.pylori-induced onset of T2DM.
基金Supported by National Natural Science Foundation of China,No.81270476the Priority Academic Program Development of Jiangsu Higher Education Institutions,JX10231801
文摘AIM:To quantify the association between Helicobacter pylori(H.pylori)infection and migraine.METHODS:A systematic literature search of PubMed and EMBASE was conducted from inception to December 2013.Studies that provided data dealing with H.pylori infection in patients with migraine,as well as healthy controls,were selected.Meta-analysis was carried out using the odds ratio(OR)with a fixed or random effects model,and a 95%CI for the OR was calculated.An unconditional logistic regression model was used to analyze potential parameters related to H.pylori prevalence.Subgroup analyses were conducted as methods of detection and evidence grade.RESULTS:Five case-control studies published between 2000 and 2013 were finally identified,involving 903 patients,with a total H.pylori infection rateof 39.31%.The prevalence of H.pylori infection was significantly greater in migraineurs than in controls(44.97%vs 33.26%,OR=1.92,95%CI:1.05-3.51,P=0.001).A sensitivity test indicated that no single study dominated the combined results.Univariate regression analysis found that publication year,geographical distribution and evidence grade were relevant to the results and were the main reason for the heterogeneity.Subgroup analysis found a significantly greater infection rate of H.pylori in Asian patients with migraine,but no statistically significant infection rate in European patients.The ORs were 3.48(95%CI:2.09-5.81,P=0.000)and 1.19(95%CI:0.86-1.65,P=0.288),respectively.CONCLUSION:The pooled data suggest a trend of more frequent H.pylori infections in patients with migraine.
