Our previous study showed that two MKN45 variants, namely MKN45Lm-and MKN 45Lm+ selected by laminin adhesion in vitro, had different abilities of invasion and metastasis in vivo (inoculated in nude mice) and in vitro ...Our previous study showed that two MKN45 variants, namely MKN45Lm-and MKN 45Lm+ selected by laminin adhesion in vitro, had different abilities of invasion and metastasis in vivo (inoculated in nude mice) and in vitro (in Boyden chamber), also had different expression of cysteine proteinase. In the present study, we investigated the cell apoptosis of this MKN45 variants by TUNEL and FACS methods. The results were shown significant different of cell apoptosis in this cell sublines. The MKN45Lm- cells had a small subdiploidy peak (3.5%) in DNA content analyzed by flow cytometry,展开更多
文摘Our previous study showed that two MKN45 variants, namely MKN45Lm-and MKN 45Lm+ selected by laminin adhesion in vitro, had different abilities of invasion and metastasis in vivo (inoculated in nude mice) and in vitro (in Boyden chamber), also had different expression of cysteine proteinase. In the present study, we investigated the cell apoptosis of this MKN45 variants by TUNEL and FACS methods. The results were shown significant different of cell apoptosis in this cell sublines. The MKN45Lm- cells had a small subdiploidy peak (3.5%) in DNA content analyzed by flow cytometry,
