[Objectives]The paper was to elucidate the specific hyperparasitic fungal species that are associated with coffee leaf rust.[Methods]Tissue isolation,sample humidification,and three-point inoculation techniques were e...[Objectives]The paper was to elucidate the specific hyperparasitic fungal species that are associated with coffee leaf rust.[Methods]Tissue isolation,sample humidification,and three-point inoculation techniques were employed to isolate,culture,and purify the hyperparasitic fungi responsible for coffee leaf rust.The purified strains were identified using traditional morphological techniques and molecular biology methods.[Results]Four strains were isolated,specifically BS21(Cladosporium cladosporioides),BS34(C.tenuissimum),BS62(C.cladosporioides),and BS75(C.colombiae).[Conclusions]The findings of this research will contribute novel insights into the biological control of coffee leaf rust.展开更多
The processability and ultimate quality of coffee(Coffea arabica)are determined by the composition of the matured fruits.The basis of genetic variation in coffee fruit quality could be explained by studying color form...The processability and ultimate quality of coffee(Coffea arabica)are determined by the composition of the matured fruits.The basis of genetic variation in coffee fruit quality could be explained by studying color formation during fruit maturation.Transcriptome profiling was conducted on matured fruits of four C.arabica varieties(orange colored fruits(ORF);purple colored fruits(PF);red colored fruits(RF)and yellow colored fruits(YF))to identify key color-regulating genes,biosynthesis pathways and transcription factors implicated in fruit color formation.A total of 39,938 genes were identified in the transcriptomes of the four C.arabica varieties.In all,2745,781 and 1224 differentially expressed genes(DEGs)were detected in YF_vs_PF,YF_vs_RF and YF_vs_ORF,respectively,with 1732 DEGs conserved among the three pairwise groups.Functional annotation of the DEGs led to the detection of 28 and 82 key genes involved in the biosynthesis of carotenoids and anthocyanins,respectively.Key transcription factors bHLH,MYB,NAC,MADS,and WRKY implicated in fruit color regulation were detected.The high expression levels of gene-LOC113688784(PSY),gene-LOC113730013(b-CHY),gene-LOC113728842(CCD7),gene-LOC113689681(NCED)and gene-LOC113729473(ABA2)in YF may have accounted for the yellow coloration.The differential expression of several anthocyanin and carotenoid-specific genes in the fruits substantially account for the purple(PF),red(RF),and orange(ORF)colorations.This study provides important insights into fruit color formation and variations in C.arabica and will help to develop coffee varieties with specific color and quality traits.展开更多
基金Supported by Yunnan Fundamental Research Projects(202301BD070001-076)Innovation Guidance and Technology-based Enterprise Cultivation Program of Yunnan Science and Technology Project(202304BP090027)Science and Technology Program of Baoshan City(2022zc01).
文摘[Objectives]The paper was to elucidate the specific hyperparasitic fungal species that are associated with coffee leaf rust.[Methods]Tissue isolation,sample humidification,and three-point inoculation techniques were employed to isolate,culture,and purify the hyperparasitic fungi responsible for coffee leaf rust.The purified strains were identified using traditional morphological techniques and molecular biology methods.[Results]Four strains were isolated,specifically BS21(Cladosporium cladosporioides),BS34(C.tenuissimum),BS62(C.cladosporioides),and BS75(C.colombiae).[Conclusions]The findings of this research will contribute novel insights into the biological control of coffee leaf rust.
基金financially supported by the Yunnan Science and Technology Introducing project(International Science and Technology Cooperation):Construction Project of Coffee Scientific and Technological Demonstration Districts in Mountainous Areas of Northern Laos(2019IB013)High-end Foreign Experts Program of Yunnan Thousand Talents Program(2019013)Yunnan provincial key programs(2019ZG00901,202002AA10007).
文摘The processability and ultimate quality of coffee(Coffea arabica)are determined by the composition of the matured fruits.The basis of genetic variation in coffee fruit quality could be explained by studying color formation during fruit maturation.Transcriptome profiling was conducted on matured fruits of four C.arabica varieties(orange colored fruits(ORF);purple colored fruits(PF);red colored fruits(RF)and yellow colored fruits(YF))to identify key color-regulating genes,biosynthesis pathways and transcription factors implicated in fruit color formation.A total of 39,938 genes were identified in the transcriptomes of the four C.arabica varieties.In all,2745,781 and 1224 differentially expressed genes(DEGs)were detected in YF_vs_PF,YF_vs_RF and YF_vs_ORF,respectively,with 1732 DEGs conserved among the three pairwise groups.Functional annotation of the DEGs led to the detection of 28 and 82 key genes involved in the biosynthesis of carotenoids and anthocyanins,respectively.Key transcription factors bHLH,MYB,NAC,MADS,and WRKY implicated in fruit color regulation were detected.The high expression levels of gene-LOC113688784(PSY),gene-LOC113730013(b-CHY),gene-LOC113728842(CCD7),gene-LOC113689681(NCED)and gene-LOC113729473(ABA2)in YF may have accounted for the yellow coloration.The differential expression of several anthocyanin and carotenoid-specific genes in the fruits substantially account for the purple(PF),red(RF),and orange(ORF)colorations.This study provides important insights into fruit color formation and variations in C.arabica and will help to develop coffee varieties with specific color and quality traits.
