[Objectives]To observe the therapeutic effect of intragastric administration of Jiangtang Shuxin recipe on diabetic heart failure(DHF)in rats and to explore its mechanism.[Methods]Fifty SD rats were randomly divided i...[Objectives]To observe the therapeutic effect of intragastric administration of Jiangtang Shuxin recipe on diabetic heart failure(DHF)in rats and to explore its mechanism.[Methods]Fifty SD rats were randomly divided into five groups,with 10 rats in each group.DHF models were prepared in the low-dose group,high-dose group,Western medicine group,and model group except the control group.Rats in the low-dose and high-dose groups were given 1.0 and 1.5 g/(kg·d)Jiangtang Shuxin recipe suspension by gavage,respectively.Rats in the Western medicine group were given gliquidone and benazepril by gavage for 2 months,and were fed with high-fat diet.Rats in the control group were fed with ordinary diet.Fasting blood glucose(FBG),serum triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),C-reactive protein(CRP),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),creatine kinase isoenzyme(CK-MB),lactate dehydrogenase(LDH),pathological morphology of myocardial tissue,NF-κB p65 protein and IκBαprotein were compared among groups.[Results]Compared with the control group,the levels of FBG,serum TG,LDL-C,CRP,IL-6,TNF-α,CK-MB and LDH increased,while the level of serum HDL-C decreased.The myocardial tissue was seriously damaged,and the expression of NF-κB p65 protein increased,while the expression of IκBαprotein decreased in the other four gruops(all P<0.05).Compared with the model group,the levels of FBG,serum TG,LDL-C,CRP,IL-6,TNF-α,CK-MB and LDH decreased,while the serum HDL-C level increased.The myocardial tissue damage was alleviated,and the expression of NF-κB p65 protein decreased,while the expression of IκBαprotein increased in the low-dose group,high-dose group and Western medicine group(all P<0.05).Compared with the Western medicine group,the levels of FBG,serum TG,LDL-C,CRP,IL-6,TNF-α,CK-MB and LDH decreased,and the level of serum HDL-C increased in the high-dose group(all P<0.05).[Conclusions]Jiangtang Shuxin recipe has a therapeutic effect on DHF in rats,with the best effect in the high-dose group.It can not only alleviate high glucose and high fat state,but also reduce myocardial injury and inflammation,and improve the pathological morphology of myocardial cells.The mechanism may be related to its inhibition of NF-кB signaling pathway.展开更多
With the continuous increase of mining in depth,the gas extraction faces the challenges of low permeability,great ground stress,high temperature and large gas pressure in coal seam.The controllable shock wave(CSW),as ...With the continuous increase of mining in depth,the gas extraction faces the challenges of low permeability,great ground stress,high temperature and large gas pressure in coal seam.The controllable shock wave(CSW),as a new method for enhancing permeability of coal seam to improve gas extraction,features in the advantages of high efficiency,eco-friendly,and low cost.In order to better utilize the CSW into gas extraction in coal mine,the mechanism and feasibility of CSW enhanced extraction need to be studied.In this paper,the basic principles,the experimental tests,the mathematical models,and the on-site tests of CSW fracturing coal seams are reviewed,thereby its future research directions are provided.Based on the different media between electrodes,the CSW can be divided into three categories:hydraulic effect,wire explosion and excitation of energetic materials by detonating wire.During the process of propagation and attenuation of the high-energy shock wave in coal,the shock wave and bubble pulsation work together to produce an enhanced permeability effect on the coal seam.The stronger the strength of the CSW is,the more cracks created in the coal is,and the greater the length,width and area of the cracks being.The repeated shock on the coal seam is conducive to the formation of complex network fracture system as well as the reduction of coal seam strength,but excessive shock frequency will also damage the coal structure,resulting in the limited effect of the enhanced gas extraction.Under the influence of ground stress,the crack propagation in coal seam will be restrained.The difference of horizontal principal stress has a significant impact on the shape,propagation direction and connectivity of the CSW induced cracks.The permeability enhancement effect of CSW is affected by the breakage degree of coal seam.The shock wave is absorbed by the broken coal,which may hinder the propagation of CSW,resulting in a poor effect of permeability enhancement.When arranging two adjacent boreholes for CSW permeability enhancement test,the spacing of boreholes should not be too close,which may lead to negative pressure mutual pulling in the early stage of drainage.At present,the accurate method for effectively predicting the CSW permeability enhanced range should be further investigated.展开更多
In order to further improve the catalytic performance of zeolite catalyst for methanol to aromatics(MTA)technology, the double-tier SAPO-34/ZSM-5/quartz composite zeolite films were successfully synthesized via hydrot...In order to further improve the catalytic performance of zeolite catalyst for methanol to aromatics(MTA)technology, the double-tier SAPO-34/ZSM-5/quartz composite zeolite films were successfully synthesized via hydrothermal crystallization. The Si/Al ratio of SAPO-34 film was used as the only variable to study this material. The composite zeolite material with 0.6Si/Al ratio of SAPO-34 has the largest mesoporous specific surface area and the most suitable acid distribution. The catalytic performance for the MTA process showed that 0.6-SAPO-34/ZSM-5/quartz film has as high as 50.3% benzene-toluenexylene selectivity and 670 min lifetime. The MTA reaction is carried out through the path we designed to effectively avoid the hydrocarbon pool circulation of ZSM-5 zeolite, so as to improve the aromatics selectivity and inhibit the occurrence of deep side reactions to a great extent. The coke deposition behavior was monitored by thermogravimetric analysis and gas chromatograph/mass spectrometer, it is found that with the increase of Si/Al ratio, the active intermediates changed from low-substituted methylbenzene to high-substituted methylbenzene, which led to the rapid deactivation of the catalyst. This work provides a possibility to employ the synergy effect of composite zeolite film synthesizing anti-carbon deposition catalyst in MTA reaction.展开更多
Hand osteoarthritis is a common heterogeneous joint disorder with unclear molecular mechanisms and no disease-modifying drugs.In this study,we performed single-cell RNA sequencing analysis to compare the cellular comp...Hand osteoarthritis is a common heterogeneous joint disorder with unclear molecular mechanisms and no disease-modifying drugs.In this study,we performed single-cell RNA sequencing analysis to compare the cellular composition and subpopulationspecific gene expression between cartilage with macroscopically confirmed osteoarthritis(n=5)and cartilage without osteoarthritis(n=5)from the interphalangeal joints of five donors.Of 105142 cells,we identified 13 subpopulations,including a novel subpopulation with inflammation-modulating potential annotated as inflammatory chondrocytes.Fibrocartilage chondrocytes exhibited extensive alteration of gene expression patterns in osteoarthritic cartilage compared with nonosteoarthritic cartilage.Both inflammatory chondrocytes and fibrocartilage chondrocytes showed a trend toward increased numbers in osteoarthritic cartilage.In these two subpopulations from osteoarthritic cartilage,the ferroptosis pathway was enriched,and expression of iron overload-related genes,e.g.,FTH1,was elevated.To verify these findings,we conducted a Mendelian randomization study using UK Biobank and a population-based cross-sectional study using data collected from Xiangya Osteoarthritis Study.Genetic predisposition toward higher expression of FTH1 mRNA significantly increased the risk of hand osteoarthritis(odds ratio=1.07,95%confidence interval:1.02–1.11)among participants(n=332668)in UK Biobank.High levels of serum ferritin(encoded by FTH1),a biomarker of body iron overload,were significantly associated with a high prevalence of hand osteoarthritis among participants(n=1241)of Xiangya Osteoarthritis Study(P-for-trend=0.037).In conclusion,our findings indicate that inflammatory and fibrocartilage chondrocytes are key subpopulations and that ferroptosis may be a key pathway in hand osteoarthritis,providing new insights into the pathophysiology and potential therapeutic targets of hand osteoarthritis.展开更多
The dynamics of wire array Z pinches are greatly affected by the initial state of the wires,which can be preconditioned by a prepulse current.Recent advances in experimental research on preconditioned wire array Z pin...The dynamics of wire array Z pinches are greatly affected by the initial state of the wires,which can be preconditioned by a prepulse current.Recent advances in experimental research on preconditioned wire array Z pinches at Xi’an Jiaotong University are presented in this paper.Single-wire explosion experiments were carried out to check the state of the preconditioning and to obtain the current parameters needed for wire gasification.Double-wire explosion experiments were conducted to investigate the temporal evolution of the density distribution of the two gasified wires.Based on the results of these experiments,a double-pulse Z-pinch facility,Qin-1,in which a 10 kA prepulse current was coupled with the 0.8 MA main current was designed and constructed.Wire arrays of different wire materials,including silver and tungsten,can be preconditioned by the prepulse current to a gaseous state.Implosion of the two preconditioned aluminum wires exhibited no ablation and little trailing mass.展开更多
In order to get a thorough understanding of non-point source pollution,it is essential to examine its temporal and spatial distribution. A physically-based distributed model,Soil and Water Assessment Tool( SWAT),was u...In order to get a thorough understanding of non-point source pollution,it is essential to examine its temporal and spatial distribution. A physically-based distributed model,Soil and Water Assessment Tool( SWAT),was used in this research,to quantitatively estimate the NPS load and analyze the temporal and spatial distributions of NPS pollution in Ashi River Basin. The results indicated that SWAT was suitable to simulate stream-flow and water quality in Ashi River Basin. Total Nitrogen which was contributed by NPS( NPS-TN) accounted for 32. 47%-62. 61%,and Total Phosphorus which was contributed by NPS( NPS-TP)accounted for 22. 30%- 57. 85% of the total load respectively. In inter-annual timescale,both NPS-TN and NPS-TP were influenced by stream-flow and fertilizer. However,when compared with fertilizer,NPS pollution was more directly affected by stream-flow. In annual timescale,NPS-TN and NPS-TP mainly occurred in flood season( from May to September). In the aspect of space,spatial differences of NPS-TN and NPS-TP were extremely significant. The spatial variations of NPS pollution were mainly influenced by land use,precipitation,soil and slope.展开更多
For the further analysis and suppression of the electric locomotives neutral-section passing overvoltage, on the basis of theoretical analysis of the neutral-section passing harmonic resonance conditions, this paper e...For the further analysis and suppression of the electric locomotives neutral-section passing overvoltage, on the basis of theoretical analysis of the neutral-section passing harmonic resonance conditions, this paper establishes simplified harmonic resonance simulation models of the electric locomotives neutral-section passing using MATLAB/Simulink, and makes the overvoltage simulation analysis of the existing electric locomotives neutral-section passing system in the event of a harmonic resonance. Results show that when the system harmonic resonance occurs, the operating overvoltage of the neutral-section passing is serious intensified by the overvoltage of the harmonic resonance, which will make the voltage of the pantograph collector head exceeding 100kV. This amplitude of the overvoltage will breakdown the air gap, which will be a serious threat to the safety operation of the electric railway. However, this kind of neutral-section passing overvoltage hasn’t cause the attention in the field and theoretical studies, which need more analysis and verification in the further study.展开更多
To cope with the rapid growth of mobile video, video providers have leveraged cloud technologies to deploy their mobile video service system for more cost-effective and scalable performance. The emergence of Software-...To cope with the rapid growth of mobile video, video providers have leveraged cloud technologies to deploy their mobile video service system for more cost-effective and scalable performance. The emergence of Software-Defined Networking(SDN) provides a promising solution to manage the underlying network. In this paper, we introduce an SDN-enabled cloud mobile video distribution architecture and propose a joint video placement, request dispatching and traffic management mechanism to improve user experience and reduce the system operational cost. We use a utility function to capture the two aspects of user experience: the level of satisfaction and average latency, and formulate the joint optimization problem as a mixed integer programming problem. We develop an optimal algorithm based on dual decomposition and prove its optimality. We conduct simulations to evaluate the performance of our algorithm and the results show that our strategy can effectively cut down the total cost and guarantee user experience.展开更多
Objective:To discuss the early imaging features of novel coronary pneumonia(NCP)and its differential diagnosis with common pneumonia for the clinical Provide relatively correct imaging diagnosis.Methods:A review of 10...Objective:To discuss the early imaging features of novel coronary pneumonia(NCP)and its differential diagnosis with common pneumonia for the clinical Provide relatively correct imaging diagnosis.Methods:A review of 10 cases of novel coronavirus pneumonia diagnosed in our hospital and surrounding counties was collected,and our hospital's 2019-2020 Common pneumonia such as influenza A and B virus pneumonia,lobar pneumonia and adenovirus confirmed by laboratory tests and abnormal chest radiographs Ten patients each with pneumonia,a total of 40 patients,were collected and their imaging features were analyzed.Results:In 10 patients with neo-coronary pneumonia,there were 30 lesions on chest CT,with typical characteristic lesions containing bronchograms within their Angiographic thickening;located in the subpleura,with grinding glass-like or combined solid changes,referred to as"extratubular halo",with multifocal and multifocal distribution.Morphology or unilobular large lamellar foci without lymph node enlargement and pleural effusion;10 cases of influenza A and B virus pneumonia in chest CT performance In nine patients,the lesions were distributed in the subpleural or along the perimeter of the bronchial vessels in the form of an analogous circular ground-glass shadow,some of which was a small piece of solid shadow,1 The case involved a single lobe of the lung and showed a large mixed ground glass image,and the CT in 10 cases of lobar pneumonia showed that they all had a single large solid lobe The variegated shadow or patchy cloud with blurred margins was triangularly altered with the tip pointing to the lung portal,and there were five cases of air-containing bronchograms.Adenoviral pneumonia is more common in infants and young children,and CT showed single or multiple grinded glass images in both lungs with patchy solid variegated shadows and lobar distribution.Conclusion:NCP and common pneumonia have certain imaging features that,in combination with laboratory tests and epidemiologic history,allow a preliminary diagnosis to be made.It has certain directions and help for clinical diagnosis.展开更多
m^(6)A methylation is the most frequent modification of mRNA in eukaryotes and plays a crucial role in cancer progression by regulating biological functions.Insulin-like growth factor 2 mRNA-binding proteins(IGF2BP)ar...m^(6)A methylation is the most frequent modification of mRNA in eukaryotes and plays a crucial role in cancer progression by regulating biological functions.Insulin-like growth factor 2 mRNA-binding proteins(IGF2BP)are newly identified m^(6)A‘readers’.They belong to a family of RNA-binding proteins,which bind to the m^(6)A sites on different RNA sequences and stabilize them to promote cancer progression.In this review,we summarize the mechanisms by which different upstream factors regulate IGF2BP in cancer.The current literature analyzed here reveals that the IGF2BP family proteins promote cancer cell proliferation,survival,and chemoresistance,inhibit apoptosis,and are also associated with cancer glycolysis,angiogenesis,and the immune response in the tumor microenvironment.Therefore,with the discovery of their role as‘readers’of m^(6)A and the characteristic re-expression of IGF2BPs in cancers,it is important to elucidate their mechanism of action in the immunosuppressive tumor microenvironment.We also describe in detail the regulatory and interaction network of the IGF2BP family in downstream target RNAs and discuss their potential clinical applications as diagnostic and prognostic markers,as well as recent advances in IGF2BP biology and associated therapeutic value.展开更多
Summary What is already known about this topic?Intestinal protozoa are common pathogens of diarrhea globally.However,the etiology of diarrhea due to intestinal protozoan infections in China is not known.What is added ...Summary What is already known about this topic?Intestinal protozoa are common pathogens of diarrhea globally.However,the etiology of diarrhea due to intestinal protozoan infections in China is not known.What is added by this report?Based on active syndromic surveillance in Shanghai,Zhenjiang,and Danyang during 2011–2015 and 2019–2021,89(1.67%)patients were infected with intestinal protozoa(Cryptosporidium spp.,Giardia duodenalis,Enterocytozoon bieneusi,and Cyclospora cayetanensis),and positivity rates statistically differed by region and age.What are the implications for public health practice?This was the most comprehensive data collection in investigating parasitic diarrheal diseases in humans.Identification of these protozoa in diarrhea will provide new perspectives for detecting hidden etiological agents of diarrhea as early as possible.展开更多
Developing high-performance nanostructured materials is key to deliver the potential of hydrovoltaic technology into practical applications.As single-component materials have approached its limit in generating hydrovo...Developing high-performance nanostructured materials is key to deliver the potential of hydrovoltaic technology into practical applications.As single-component materials have approached its limit in generating hydrovoltaic electricity,the development of multi-component hydrovoltaic materials has been necessary in continuously boosting the electricity output.Here,we report a hydrovoltaic material by integrating reduced graphene oxides and polypyrrole nanoparticles(rGO/PPy),where the rGO contributes improved conductivity and large specific surface area while PPy nanoparticles enable enhanced interaction with water.The device fabricated with this material generates a short-circuit current of 6μA as well as a maximum power density of over 1μW/cm3 from natural evaporation of water.And the substantial ion-PPy interaction enables robust voltage generation from evaporation of various salt solutions.Moreover,an outstanding scaling ability is demonstrated by connecting 10 devices in series that generate a sustainable voltage of up to~2.5 V,sufficing to power many commercial devices,e.g.LED bulb and LCD screen.展开更多
The last decade has witnessed the emergence of hydrovoltaic technology,which can harvest electricity from different forms of water movement,such as raindrops,waves,flows,moisture,and natural evaporation.In particular,...The last decade has witnessed the emergence of hydrovoltaic technology,which can harvest electricity from different forms of water movement,such as raindrops,waves,flows,moisture,and natural evaporation.In particular,the evaporation-induced hydrovoltaic effect received great attention since its discovery in 2017 due to its negative heat emission property.Nevertheless,the influence of electrode reactions in evaporation-induced power generation is not negligible due to the chemical reaction between active metal electrodes and water,which leads to“exceptional”power generation.Herein,we designed a series of experiments based on air-laid paper devices with electrodes of different activities as the top and bottom electrodes.To verify the contribution of electrodes,we compared the output performance of different electrode combinations when the device was partially-wetted and fully-wetted.The device hydrophilicity,salt concentration,and acidity or basicity of solutions were also comprehensively investigated.It is demonstrated that the chemical reaction of active metals(Zn,Cu,Ag,etc.)with different aqueous solutions can generate considerable electrical energy and significantly distort the device performance,especially for Zn electrodes with an output voltage from~1.26 to~1.52 V and current from~1.24 to~75.69μA.To promote the long-term development of hydrovoltaic technology,we recommend use of inert electrodes in hydrovoltaic studies,such as Au and Pt,especially in water and moisture environment.展开更多
Photodynamic therapy(PDT)is a promising non-invasive therapy approach for various diseases including malignant tumor.The process of PDT involves three interrelated aspects,namely photosensitizer(PS),light source,and o...Photodynamic therapy(PDT)is a promising non-invasive therapy approach for various diseases including malignant tumor.The process of PDT involves three interrelated aspects,namely photosensitizer(PS),light source,and oxygen,among which PS is the decisive factor that determines its anticancer efficiency.There exist some defects in currently applied PDT,such as inadequate production of reactive oxygen species(ROS),poor penetration of exciting light,insufficient oxygen supply,and nonselective distribution of PS.With unique physicochemical and optical properties,two-dimensional nanomaterials(2DNMs)have aroused great interest in biomedical fields.2DNMs-based PDT is promising to significantly improve antitumor efficacy compared to conventional PDT.In this review,we will firstly introduce the underlying mechanism of PDT and how 2DNMs are absorbed and distribute inside tumor cells.After that,we will not only illustrate how 2DNMs-based PDT can enhance tumor-killing efficacy and minimize side-effects through conquering the above-mentioned defects of conventional PDT and the preparation process of 2DNMs,but also elaborate recent advances about 2DNMs-based PDT.Lastly,we will summarize the challenges and future prospects of 2DNMs-based PDT.展开更多
Stroke at the acute stage is a major cause of disability in adults, and is associated with dysfunction of brain networks. However, the mechanisms underlying changes in brain connectivity in stroke are far from fully e...Stroke at the acute stage is a major cause of disability in adults, and is associated with dysfunction of brain networks. However, the mechanisms underlying changes in brain connectivity in stroke are far from fully elucidated. In the present study, we investigated brain metabolism and metabolic connectivity in a rat ischemic stroke model of middle cerebral artery occlusion （MCAO） at the acute stage using 18F-fluorodeoxyglucose positron emission tomography. Voxel-wise analysis showed decreased metabolism mainly in the ipsilesional hemi- sphere, and increased metabolism mainly in the contrale- sional cerebellum. We used further metabolic connectivity analysis to explore the brain metabolic network in MCAO. Compared to sham controls, rats with MCAO showed most significantly reduced nodal and local efficiency in the ipsilesional striatum. In addition, the MCAO group showed decreased metabolic central connection of the ipsilesional striatum with the ipsilesional cerebellum, ipsilesional hippocampus, and bilateral hypothalamus. Taken together, the present study demonstrated abnormal metabolic con- nectivity in rats at the acute stage of ischemic stroke, which might provide insight into clinical research.展开更多
Our study sought to assess how much phosphorus（P） runoff from paddy fields could be cut down by fertilizer management and inoculation with arbuscular mycorrhizal fungi. A field experiment was conducted in Lalin Rive...Our study sought to assess how much phosphorus（P） runoff from paddy fields could be cut down by fertilizer management and inoculation with arbuscular mycorrhizal fungi. A field experiment was conducted in Lalin River basin, in the northeast China： six nitrogen-phosphorus-potassium fertilizer levels were provided（0, 20%, 40%, 60%, 80%, and 100% of the recommended fertilizer supply）, with or without inoculation with Glomus mosseae. The volume and concentrations of particle P（PP） and dissolved P（DP） were measured for each runoff during the rice growing season. It was found that the seasonal P runoff, including DP and PP, under the local fertilization was 3.7 kg/ha, with PP, rather than DP, being the main form of P in runoff water. Additionally, the seasonal P runoff dropped only by 8.9% when fertilization decreased by 20%; rice yields decreased with declining fertilization. We also found that inoculation increased rice yields and decreased P runoff at each fertilizer level and these effects were lower under higher fertilization. Conclusively, while rice yields were guaranteed arbuscular mycorrhizal inoculation and fertilizer management would play a key role in reducing P runoff from paddy fields.展开更多
Positron emission tomography （PET） imaging of functional metabolism has been widely used to investigate functional recovery and to evaluate therapeutic efficacy after stroke. The voxel intensity of a PET image is th...Positron emission tomography （PET） imaging of functional metabolism has been widely used to investigate functional recovery and to evaluate therapeutic efficacy after stroke. The voxel intensity of a PET image is the most important indicator of cellular activity, but is affected by other factors such as the basal metabolic ratio of each subject. In order to locate dysfunctional regions accurately, intensity normalization by a scale factor is a prerequisite in the data analysis, for which the global mean value is most widely used. However, this is unsuitable for stroke studies. Alternatively, a specified scale factor calculated from a reference region is also used, comprising neither hyper- nor hypo-metabolic voxels. But there is no such recognized reference region for stroke studies. Therefore, we proposed a totally data-driven automatic method for unbiased scale factor generation. This factor was generated iteratively until the residual deviation of two adjacent scale factors was reduced by 〈 5%. Moreover, both simulated and real stroke data were used for evaluation, and these suggested that our proposed unbiased scale factor has better sensi- tivity and accuracy for stroke studies.展开更多
What is already known about this topic?Both alveolar echinococcosis(AE)and cystic echinococcosis are endemic in China,among which alveolar echinococcosis has a very high mortality rate.What is added by this report?The...What is already known about this topic?Both alveolar echinococcosis(AE)and cystic echinococcosis are endemic in China,among which alveolar echinococcosis has a very high mortality rate.What is added by this report?The survey results showed the prevalence and scope of AE in China and identified high-risk groups including children,monks,herdsmen and illiterate people.At the same time,all the cases found in the survey(more than 90%of the patients did not go to the hospital for diagnosis and treatment before survey)were promptly diagnosed and treated.What are the implications for public health practice?This study provides information for the development of a plan for AE prevention and control and for the implementation of interventions targeted to high-risk populations.展开更多
We report the synthesis of cellulose membranes from balsa wood with an exceptionally high responsivity to humidity change by chemical processing and mechanical compression.By varying the ambient humidity,the produced ...We report the synthesis of cellulose membranes from balsa wood with an exceptionally high responsivity to humidity change by chemical processing and mechanical compression.By varying the ambient humidity,the produced cellulose membranes can provide a variety of predetermined deformations,such as curve,s-like deformation and curl.The high humidity responsivity is originated from a self-maintained moisture gradient induced by an asymmetrical design of membrane surfaces,aided by the hygroscopic swelling of the cellulose.The moisture-driven actuators are then demonstrated as a three-finger gripper that can grab,hold and release objects 40 times the weight of its own.The combination of natural wood and stimuli-responsive behavior open a way to designing smart structures,actuators and soft robots with environmentally friendly,recyclable and biocompatible materials.展开更多
Background and aims:Berberine is one of the most promising clinically tested natural alkaloids,and immunotherapy using natural killer(NK)cells is a potentially effective treatment for hepatocellular carcinoma(HCC).Thi...Background and aims:Berberine is one of the most promising clinically tested natural alkaloids,and immunotherapy using natural killer(NK)cells is a potentially effective treatment for hepatocellular carcinoma(HCC).This study aims to elucidate the effect of berberine on the anti-HCC effect of NK92-MI cells.Materials and methods:Human HCC SMMC-7721 and Hep3B cells were co-incubated with NK92-MI cells,berberine(60 or 80 mmol/L),or their combination for 36 h.To evaluate the killing effect of NK92-MI cells on HCC cells,the release of lactate dehydrogenase(LDH)in HCC cells was measured.The anti-tumor effects of berberine,NK92-MI cells,and their combinations were evaluated by MTS,EdU,Tunel,and Western blot assays.A male BALB/c nude mouse subcutaneous tumor model was used to investigate the anti-HCC effect of berberine and NK92-MI cells in vivo.Results:The LDH assay showed that berberine enhanced the cytotoxicity of NK92-MI cells on HCC cells.The combination of berberine and NK92-MI cells demonstrated more obvious anti-HCC effect than did the berberine or NK92-MI single treatment in inhibiting cell proliferation and inducing apoptosis both in vitro and in vivo.Mechanistically,the expression of programmed cell death-ligand 1(PD-L1)in HCC cells was upregulated after co-culture with NK-92MI cells.PD-L1 expression was knocked down,thereby inhibiting the proliferation and promoting apoptosis of HCC cells,and inhibited by berberine that blocked the secretion of interferon gamma(IFN-g),thereby enhancing the anti-tumor effect of NK92-MI cells.Conclusions:Current data show that the immunomodulatory role of berberine is to enhance the cytotoxic effect of NK92-MI cells and inhibit tumor immune escape by reducing the expression of PD-L1.Our study provides a rationale for the clinical application of berberine in combination with NK cells for the treatment of HCC.展开更多
文摘[Objectives]To observe the therapeutic effect of intragastric administration of Jiangtang Shuxin recipe on diabetic heart failure(DHF)in rats and to explore its mechanism.[Methods]Fifty SD rats were randomly divided into five groups,with 10 rats in each group.DHF models were prepared in the low-dose group,high-dose group,Western medicine group,and model group except the control group.Rats in the low-dose and high-dose groups were given 1.0 and 1.5 g/(kg·d)Jiangtang Shuxin recipe suspension by gavage,respectively.Rats in the Western medicine group were given gliquidone and benazepril by gavage for 2 months,and were fed with high-fat diet.Rats in the control group were fed with ordinary diet.Fasting blood glucose(FBG),serum triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),C-reactive protein(CRP),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),creatine kinase isoenzyme(CK-MB),lactate dehydrogenase(LDH),pathological morphology of myocardial tissue,NF-κB p65 protein and IκBαprotein were compared among groups.[Results]Compared with the control group,the levels of FBG,serum TG,LDL-C,CRP,IL-6,TNF-α,CK-MB and LDH increased,while the level of serum HDL-C decreased.The myocardial tissue was seriously damaged,and the expression of NF-κB p65 protein increased,while the expression of IκBαprotein decreased in the other four gruops(all P<0.05).Compared with the model group,the levels of FBG,serum TG,LDL-C,CRP,IL-6,TNF-α,CK-MB and LDH decreased,while the serum HDL-C level increased.The myocardial tissue damage was alleviated,and the expression of NF-κB p65 protein decreased,while the expression of IκBαprotein increased in the low-dose group,high-dose group and Western medicine group(all P<0.05).Compared with the Western medicine group,the levels of FBG,serum TG,LDL-C,CRP,IL-6,TNF-α,CK-MB and LDH decreased,and the level of serum HDL-C increased in the high-dose group(all P<0.05).[Conclusions]Jiangtang Shuxin recipe has a therapeutic effect on DHF in rats,with the best effect in the high-dose group.It can not only alleviate high glucose and high fat state,but also reduce myocardial injury and inflammation,and improve the pathological morphology of myocardial cells.The mechanism may be related to its inhibition of NF-кB signaling pathway.
基金National Natural Science Foundation of China(52004117,52174117 and 52074146)Postdoctoral Science Foundation of China(2021T140290 and 2020M680975)Basic scientific research project of Liaoning Provincial Department of Education(JYTZD2023073).
文摘With the continuous increase of mining in depth,the gas extraction faces the challenges of low permeability,great ground stress,high temperature and large gas pressure in coal seam.The controllable shock wave(CSW),as a new method for enhancing permeability of coal seam to improve gas extraction,features in the advantages of high efficiency,eco-friendly,and low cost.In order to better utilize the CSW into gas extraction in coal mine,the mechanism and feasibility of CSW enhanced extraction need to be studied.In this paper,the basic principles,the experimental tests,the mathematical models,and the on-site tests of CSW fracturing coal seams are reviewed,thereby its future research directions are provided.Based on the different media between electrodes,the CSW can be divided into three categories:hydraulic effect,wire explosion and excitation of energetic materials by detonating wire.During the process of propagation and attenuation of the high-energy shock wave in coal,the shock wave and bubble pulsation work together to produce an enhanced permeability effect on the coal seam.The stronger the strength of the CSW is,the more cracks created in the coal is,and the greater the length,width and area of the cracks being.The repeated shock on the coal seam is conducive to the formation of complex network fracture system as well as the reduction of coal seam strength,but excessive shock frequency will also damage the coal structure,resulting in the limited effect of the enhanced gas extraction.Under the influence of ground stress,the crack propagation in coal seam will be restrained.The difference of horizontal principal stress has a significant impact on the shape,propagation direction and connectivity of the CSW induced cracks.The permeability enhancement effect of CSW is affected by the breakage degree of coal seam.The shock wave is absorbed by the broken coal,which may hinder the propagation of CSW,resulting in a poor effect of permeability enhancement.When arranging two adjacent boreholes for CSW permeability enhancement test,the spacing of boreholes should not be too close,which may lead to negative pressure mutual pulling in the early stage of drainage.At present,the accurate method for effectively predicting the CSW permeability enhanced range should be further investigated.
基金supported by the National Natural Science Foundation of China (51974312, 51974308)the National Key Research & Development Program of China (2019YFE0100100)。
文摘In order to further improve the catalytic performance of zeolite catalyst for methanol to aromatics(MTA)technology, the double-tier SAPO-34/ZSM-5/quartz composite zeolite films were successfully synthesized via hydrothermal crystallization. The Si/Al ratio of SAPO-34 film was used as the only variable to study this material. The composite zeolite material with 0.6Si/Al ratio of SAPO-34 has the largest mesoporous specific surface area and the most suitable acid distribution. The catalytic performance for the MTA process showed that 0.6-SAPO-34/ZSM-5/quartz film has as high as 50.3% benzene-toluenexylene selectivity and 670 min lifetime. The MTA reaction is carried out through the path we designed to effectively avoid the hydrocarbon pool circulation of ZSM-5 zeolite, so as to improve the aromatics selectivity and inhibit the occurrence of deep side reactions to a great extent. The coke deposition behavior was monitored by thermogravimetric analysis and gas chromatograph/mass spectrometer, it is found that with the increase of Si/Al ratio, the active intermediates changed from low-substituted methylbenzene to high-substituted methylbenzene, which led to the rapid deactivation of the catalyst. This work provides a possibility to employ the synergy effect of composite zeolite film synthesizing anti-carbon deposition catalyst in MTA reaction.
基金supported by the National Natural Science Foundation of China(81930071,82072502)the National Natural Science Foundation Regional Innovation and Development Joint Fund(U21A20352)+5 种基金the National Key Research and Development Project(2022YFC3601900,2022YFC2505500)the Project Program of National Clinical Research Center for Geriatric Disorders(Xiangya Hospital,2021LNJJ06,2022LNJJ07)the Natural Science Foundation of Hunan Province(2022JJ20100)the Key Research and Development Program of Hunan Province(2021SK2017)the Science and Technology Innovation Program of Hunan Province(2022RC3075)the Central South University Innovation-Driven Research Program(2023CXQD031)。
文摘Hand osteoarthritis is a common heterogeneous joint disorder with unclear molecular mechanisms and no disease-modifying drugs.In this study,we performed single-cell RNA sequencing analysis to compare the cellular composition and subpopulationspecific gene expression between cartilage with macroscopically confirmed osteoarthritis(n=5)and cartilage without osteoarthritis(n=5)from the interphalangeal joints of five donors.Of 105142 cells,we identified 13 subpopulations,including a novel subpopulation with inflammation-modulating potential annotated as inflammatory chondrocytes.Fibrocartilage chondrocytes exhibited extensive alteration of gene expression patterns in osteoarthritic cartilage compared with nonosteoarthritic cartilage.Both inflammatory chondrocytes and fibrocartilage chondrocytes showed a trend toward increased numbers in osteoarthritic cartilage.In these two subpopulations from osteoarthritic cartilage,the ferroptosis pathway was enriched,and expression of iron overload-related genes,e.g.,FTH1,was elevated.To verify these findings,we conducted a Mendelian randomization study using UK Biobank and a population-based cross-sectional study using data collected from Xiangya Osteoarthritis Study.Genetic predisposition toward higher expression of FTH1 mRNA significantly increased the risk of hand osteoarthritis(odds ratio=1.07,95%confidence interval:1.02–1.11)among participants(n=332668)in UK Biobank.High levels of serum ferritin(encoded by FTH1),a biomarker of body iron overload,were significantly associated with a high prevalence of hand osteoarthritis among participants(n=1241)of Xiangya Osteoarthritis Study(P-for-trend=0.037).In conclusion,our findings indicate that inflammatory and fibrocartilage chondrocytes are key subpopulations and that ferroptosis may be a key pathway in hand osteoarthritis,providing new insights into the pathophysiology and potential therapeutic targets of hand osteoarthritis.
文摘The dynamics of wire array Z pinches are greatly affected by the initial state of the wires,which can be preconditioned by a prepulse current.Recent advances in experimental research on preconditioned wire array Z pinches at Xi’an Jiaotong University are presented in this paper.Single-wire explosion experiments were carried out to check the state of the preconditioning and to obtain the current parameters needed for wire gasification.Double-wire explosion experiments were conducted to investigate the temporal evolution of the density distribution of the two gasified wires.Based on the results of these experiments,a double-pulse Z-pinch facility,Qin-1,in which a 10 kA prepulse current was coupled with the 0.8 MA main current was designed and constructed.Wire arrays of different wire materials,including silver and tungsten,can be preconditioned by the prepulse current to a gaseous state.Implosion of the two preconditioned aluminum wires exhibited no ablation and little trailing mass.
基金Sponsored by the National Natural Science Foundation of China(Grant No.51179041)the Major Science and Technology Program for Water Pollution Control and Treatment(Grant No.2013ZX07201007)+2 种基金Natural Science Foundation of Heilongjiang Province,China(Grant No.E201206)Special Fund for Science and Technology Innovation of Harbin(Grant No.2012RFLXS026)the State Key Lab of Urban Water Resource and Environment(Harbin Institute of Technology)(Grant No.2014TS05)
文摘In order to get a thorough understanding of non-point source pollution,it is essential to examine its temporal and spatial distribution. A physically-based distributed model,Soil and Water Assessment Tool( SWAT),was used in this research,to quantitatively estimate the NPS load and analyze the temporal and spatial distributions of NPS pollution in Ashi River Basin. The results indicated that SWAT was suitable to simulate stream-flow and water quality in Ashi River Basin. Total Nitrogen which was contributed by NPS( NPS-TN) accounted for 32. 47%-62. 61%,and Total Phosphorus which was contributed by NPS( NPS-TP)accounted for 22. 30%- 57. 85% of the total load respectively. In inter-annual timescale,both NPS-TN and NPS-TP were influenced by stream-flow and fertilizer. However,when compared with fertilizer,NPS pollution was more directly affected by stream-flow. In annual timescale,NPS-TN and NPS-TP mainly occurred in flood season( from May to September). In the aspect of space,spatial differences of NPS-TN and NPS-TP were extremely significant. The spatial variations of NPS pollution were mainly influenced by land use,precipitation,soil and slope.
文摘For the further analysis and suppression of the electric locomotives neutral-section passing overvoltage, on the basis of theoretical analysis of the neutral-section passing harmonic resonance conditions, this paper establishes simplified harmonic resonance simulation models of the electric locomotives neutral-section passing using MATLAB/Simulink, and makes the overvoltage simulation analysis of the existing electric locomotives neutral-section passing system in the event of a harmonic resonance. Results show that when the system harmonic resonance occurs, the operating overvoltage of the neutral-section passing is serious intensified by the overvoltage of the harmonic resonance, which will make the voltage of the pantograph collector head exceeding 100kV. This amplitude of the overvoltage will breakdown the air gap, which will be a serious threat to the safety operation of the electric railway. However, this kind of neutral-section passing overvoltage hasn’t cause the attention in the field and theoretical studies, which need more analysis and verification in the further study.
基金supported by the State Key Program of National Natural Science Foundation of China(Grant No.61233003)National Natural Science Foundation of China(Grant No.61503358)
文摘To cope with the rapid growth of mobile video, video providers have leveraged cloud technologies to deploy their mobile video service system for more cost-effective and scalable performance. The emergence of Software-Defined Networking(SDN) provides a promising solution to manage the underlying network. In this paper, we introduce an SDN-enabled cloud mobile video distribution architecture and propose a joint video placement, request dispatching and traffic management mechanism to improve user experience and reduce the system operational cost. We use a utility function to capture the two aspects of user experience: the level of satisfaction and average latency, and formulate the joint optimization problem as a mixed integer programming problem. We develop an optimal algorithm based on dual decomposition and prove its optimality. We conduct simulations to evaluate the performance of our algorithm and the results show that our strategy can effectively cut down the total cost and guarantee user experience.
文摘Objective:To discuss the early imaging features of novel coronary pneumonia(NCP)and its differential diagnosis with common pneumonia for the clinical Provide relatively correct imaging diagnosis.Methods:A review of 10 cases of novel coronavirus pneumonia diagnosed in our hospital and surrounding counties was collected,and our hospital's 2019-2020 Common pneumonia such as influenza A and B virus pneumonia,lobar pneumonia and adenovirus confirmed by laboratory tests and abnormal chest radiographs Ten patients each with pneumonia,a total of 40 patients,were collected and their imaging features were analyzed.Results:In 10 patients with neo-coronary pneumonia,there were 30 lesions on chest CT,with typical characteristic lesions containing bronchograms within their Angiographic thickening;located in the subpleura,with grinding glass-like or combined solid changes,referred to as"extratubular halo",with multifocal and multifocal distribution.Morphology or unilobular large lamellar foci without lymph node enlargement and pleural effusion;10 cases of influenza A and B virus pneumonia in chest CT performance In nine patients,the lesions were distributed in the subpleural or along the perimeter of the bronchial vessels in the form of an analogous circular ground-glass shadow,some of which was a small piece of solid shadow,1 The case involved a single lobe of the lung and showed a large mixed ground glass image,and the CT in 10 cases of lobar pneumonia showed that they all had a single large solid lobe The variegated shadow or patchy cloud with blurred margins was triangularly altered with the tip pointing to the lung portal,and there were five cases of air-containing bronchograms.Adenoviral pneumonia is more common in infants and young children,and CT showed single or multiple grinded glass images in both lungs with patchy solid variegated shadows and lobar distribution.Conclusion:NCP and common pneumonia have certain imaging features that,in combination with laboratory tests and epidemiologic history,allow a preliminary diagnosis to be made.It has certain directions and help for clinical diagnosis.
基金supported by grants from the Liaoning Nature Science Foundationof China(No.2022JH2/101300042)The National Natural Science Foundation of China(No.82173194,81672877)+1 种基金the Key Research Project of Liaoning Provincial Department of Education of China(No.ZD2020004)2018 Youth Backbone Support Program of China Medical University(No.QGZ2018063).
文摘m^(6)A methylation is the most frequent modification of mRNA in eukaryotes and plays a crucial role in cancer progression by regulating biological functions.Insulin-like growth factor 2 mRNA-binding proteins(IGF2BP)are newly identified m^(6)A‘readers’.They belong to a family of RNA-binding proteins,which bind to the m^(6)A sites on different RNA sequences and stabilize them to promote cancer progression.In this review,we summarize the mechanisms by which different upstream factors regulate IGF2BP in cancer.The current literature analyzed here reveals that the IGF2BP family proteins promote cancer cell proliferation,survival,and chemoresistance,inhibit apoptosis,and are also associated with cancer glycolysis,angiogenesis,and the immune response in the tumor microenvironment.Therefore,with the discovery of their role as‘readers’of m^(6)A and the characteristic re-expression of IGF2BPs in cancers,it is important to elucidate their mechanism of action in the immunosuppressive tumor microenvironment.We also describe in detail the regulatory and interaction network of the IGF2BP family in downstream target RNAs and discuss their potential clinical applications as diagnostic and prognostic markers,as well as recent advances in IGF2BP biology and associated therapeutic value.
基金Supported by the National Science and Technology Major Program of China(Nos.2018ZX10713001-004,2012ZX10004-201,and 2009ZX10004-201)the National Nature Science Foundation of China(Nos.82072307 and 81772224)+1 种基金the Three-Year Public Health Action Plan(2020-2022)of Shanghai(No.GWV-10.1-XK13)the National Key R&D Program of China No.2021YFC2300900).
文摘Summary What is already known about this topic?Intestinal protozoa are common pathogens of diarrhea globally.However,the etiology of diarrhea due to intestinal protozoan infections in China is not known.What is added by this report?Based on active syndromic surveillance in Shanghai,Zhenjiang,and Danyang during 2011–2015 and 2019–2021,89(1.67%)patients were infected with intestinal protozoa(Cryptosporidium spp.,Giardia duodenalis,Enterocytozoon bieneusi,and Cyclospora cayetanensis),and positivity rates statistically differed by region and age.What are the implications for public health practice?This was the most comprehensive data collection in investigating parasitic diarrheal diseases in humans.Identification of these protozoa in diarrhea will provide new perspectives for detecting hidden etiological agents of diarrhea as early as possible.
基金The work was supported by the Fundamental Research Funds for the Central Universities[NJ2022002]National Key Research and Development Program of China[2019YFA0705400]+1 种基金National NSF of China[1221101035,12225205,22073048]Research Fund of State Key Laboratory of Mechanics and Control of Mechanical Structures[MCMS-I-0422K01].
文摘Developing high-performance nanostructured materials is key to deliver the potential of hydrovoltaic technology into practical applications.As single-component materials have approached its limit in generating hydrovoltaic electricity,the development of multi-component hydrovoltaic materials has been necessary in continuously boosting the electricity output.Here,we report a hydrovoltaic material by integrating reduced graphene oxides and polypyrrole nanoparticles(rGO/PPy),where the rGO contributes improved conductivity and large specific surface area while PPy nanoparticles enable enhanced interaction with water.The device fabricated with this material generates a short-circuit current of 6μA as well as a maximum power density of over 1μW/cm3 from natural evaporation of water.And the substantial ion-PPy interaction enables robust voltage generation from evaporation of various salt solutions.Moreover,an outstanding scaling ability is demonstrated by connecting 10 devices in series that generate a sustainable voltage of up to~2.5 V,sufficing to power many commercial devices,e.g.LED bulb and LCD screen.
基金supported by the National and Jiangsu Province NSF(Nos.T2293691 and BK20212008)of ChinaNational Key Research and Development Program of China(No.2019YFA0705400)+2 种基金the Research Fund of State Key Laboratory of Mechanics and Control of Mechanical Structures(No.MCMS-I-0422K01)the Fundamental Research Funds for the Central Universities(No.NJ2022002)the Fund of Prospective Layout of Scientific Research for NUAA(Nanjing University of Aeronautics and Astronautics).
文摘The last decade has witnessed the emergence of hydrovoltaic technology,which can harvest electricity from different forms of water movement,such as raindrops,waves,flows,moisture,and natural evaporation.In particular,the evaporation-induced hydrovoltaic effect received great attention since its discovery in 2017 due to its negative heat emission property.Nevertheless,the influence of electrode reactions in evaporation-induced power generation is not negligible due to the chemical reaction between active metal electrodes and water,which leads to“exceptional”power generation.Herein,we designed a series of experiments based on air-laid paper devices with electrodes of different activities as the top and bottom electrodes.To verify the contribution of electrodes,we compared the output performance of different electrode combinations when the device was partially-wetted and fully-wetted.The device hydrophilicity,salt concentration,and acidity or basicity of solutions were also comprehensively investigated.It is demonstrated that the chemical reaction of active metals(Zn,Cu,Ag,etc.)with different aqueous solutions can generate considerable electrical energy and significantly distort the device performance,especially for Zn electrodes with an output voltage from~1.26 to~1.52 V and current from~1.24 to~75.69μA.To promote the long-term development of hydrovoltaic technology,we recommend use of inert electrodes in hydrovoltaic studies,such as Au and Pt,especially in water and moisture environment.
基金The research was partially supported by the National Natural Science Fundation of China(Nos.61875138,61435010,and 61961136001)Science and Technology Innovation Commission of Shenzhen(No.JCYJ20170811093453105).Authors also acknowledge the support from Instrumental Analysis Center of Shenzhen University(Xili Campus).
文摘Photodynamic therapy(PDT)is a promising non-invasive therapy approach for various diseases including malignant tumor.The process of PDT involves three interrelated aspects,namely photosensitizer(PS),light source,and oxygen,among which PS is the decisive factor that determines its anticancer efficiency.There exist some defects in currently applied PDT,such as inadequate production of reactive oxygen species(ROS),poor penetration of exciting light,insufficient oxygen supply,and nonselective distribution of PS.With unique physicochemical and optical properties,two-dimensional nanomaterials(2DNMs)have aroused great interest in biomedical fields.2DNMs-based PDT is promising to significantly improve antitumor efficacy compared to conventional PDT.In this review,we will firstly introduce the underlying mechanism of PDT and how 2DNMs are absorbed and distribute inside tumor cells.After that,we will not only illustrate how 2DNMs-based PDT can enhance tumor-killing efficacy and minimize side-effects through conquering the above-mentioned defects of conventional PDT and the preparation process of 2DNMs,but also elaborate recent advances about 2DNMs-based PDT.Lastly,we will summarize the challenges and future prospects of 2DNMs-based PDT.
基金supported by grants from the National Natural Science Foundation of China(81471741,81471728,and 81671770)
文摘Stroke at the acute stage is a major cause of disability in adults, and is associated with dysfunction of brain networks. However, the mechanisms underlying changes in brain connectivity in stroke are far from fully elucidated. In the present study, we investigated brain metabolism and metabolic connectivity in a rat ischemic stroke model of middle cerebral artery occlusion （MCAO） at the acute stage using 18F-fluorodeoxyglucose positron emission tomography. Voxel-wise analysis showed decreased metabolism mainly in the ipsilesional hemi- sphere, and increased metabolism mainly in the contrale- sional cerebellum. We used further metabolic connectivity analysis to explore the brain metabolic network in MCAO. Compared to sham controls, rats with MCAO showed most significantly reduced nodal and local efficiency in the ipsilesional striatum. In addition, the MCAO group showed decreased metabolic central connection of the ipsilesional striatum with the ipsilesional cerebellum, ipsilesional hippocampus, and bilateral hypothalamus. Taken together, the present study demonstrated abnormal metabolic con- nectivity in rats at the acute stage of ischemic stroke, which might provide insight into clinical research.
基金supported by National Natural Science Foundation of China (51179041)Major Science and Technology Program for Water Pollution Control and Treatment (No. 2013ZX07201003)the State Key Lab of Urban Water Resource and Environment (Harbin Institute of Technology) (No. 2014TS05)
文摘Our study sought to assess how much phosphorus（P） runoff from paddy fields could be cut down by fertilizer management and inoculation with arbuscular mycorrhizal fungi. A field experiment was conducted in Lalin River basin, in the northeast China： six nitrogen-phosphorus-potassium fertilizer levels were provided（0, 20%, 40%, 60%, 80%, and 100% of the recommended fertilizer supply）, with or without inoculation with Glomus mosseae. The volume and concentrations of particle P（PP） and dissolved P（DP） were measured for each runoff during the rice growing season. It was found that the seasonal P runoff, including DP and PP, under the local fertilization was 3.7 kg/ha, with PP, rather than DP, being the main form of P in runoff water. Additionally, the seasonal P runoff dropped only by 8.9% when fertilization decreased by 20%; rice yields decreased with declining fertilization. We also found that inoculation increased rice yields and decreased P runoff at each fertilizer level and these effects were lower under higher fertilization. Conclusively, while rice yields were guaranteed arbuscular mycorrhizal inoculation and fertilizer management would play a key role in reducing P runoff from paddy fields.
基金supported by the National Natural Science Foundation of China(81471741,81471728,and81671770)
文摘Positron emission tomography （PET） imaging of functional metabolism has been widely used to investigate functional recovery and to evaluate therapeutic efficacy after stroke. The voxel intensity of a PET image is the most important indicator of cellular activity, but is affected by other factors such as the basal metabolic ratio of each subject. In order to locate dysfunctional regions accurately, intensity normalization by a scale factor is a prerequisite in the data analysis, for which the global mean value is most widely used. However, this is unsuitable for stroke studies. Alternatively, a specified scale factor calculated from a reference region is also used, comprising neither hyper- nor hypo-metabolic voxels. But there is no such recognized reference region for stroke studies. Therefore, we proposed a totally data-driven automatic method for unbiased scale factor generation. This factor was generated iteratively until the residual deviation of two adjacent scale factors was reduced by 〈 5%. Moreover, both simulated and real stroke data were used for evaluation, and these suggested that our proposed unbiased scale factor has better sensi- tivity and accuracy for stroke studies.
文摘What is already known about this topic?Both alveolar echinococcosis(AE)and cystic echinococcosis are endemic in China,among which alveolar echinococcosis has a very high mortality rate.What is added by this report?The survey results showed the prevalence and scope of AE in China and identified high-risk groups including children,monks,herdsmen and illiterate people.At the same time,all the cases found in the survey(more than 90%of the patients did not go to the hospital for diagnosis and treatment before survey)were promptly diagnosed and treated.What are the implications for public health practice?This study provides information for the development of a plan for AE prevention and control and for the implementation of interventions targeted to high-risk populations.
基金This work was supported by National Key Research and Development Program of China(2019YFA0705400)National Natural Science Foundation of China(11772153,22073048)+3 种基金the Natural Science Foundation of Jiangsu Province(BK20190018)the Research Fund of State Key Laboratory of Mechanics and Control of Mechanical Structures(MCMS-E-0420K01)the Fundamental Research Funds for the Central Universities(NJ2020003,NZ2020001)a Project by the Priority Academic Program Development of Jiangsu Higher Education Institutions。
文摘We report the synthesis of cellulose membranes from balsa wood with an exceptionally high responsivity to humidity change by chemical processing and mechanical compression.By varying the ambient humidity,the produced cellulose membranes can provide a variety of predetermined deformations,such as curve,s-like deformation and curl.The high humidity responsivity is originated from a self-maintained moisture gradient induced by an asymmetrical design of membrane surfaces,aided by the hygroscopic swelling of the cellulose.The moisture-driven actuators are then demonstrated as a three-finger gripper that can grab,hold and release objects 40 times the weight of its own.The combination of natural wood and stimuli-responsive behavior open a way to designing smart structures,actuators and soft robots with environmentally friendly,recyclable and biocompatible materials.
基金This study was supported by grants from the General Project of Guangdong Natural Science Foundation(2021A1515010795,2020A1515011255)to H.Yangthe National Natural Science Foundation of China(no.81372634)to H.Yang+1 种基金the Medical Science and Technology Research Foundation of Guangdong Province(B2020114)to C.Guthe Guangzhou Health Science and Technology Project(20201A010054)to C.Gu.
文摘Background and aims:Berberine is one of the most promising clinically tested natural alkaloids,and immunotherapy using natural killer(NK)cells is a potentially effective treatment for hepatocellular carcinoma(HCC).This study aims to elucidate the effect of berberine on the anti-HCC effect of NK92-MI cells.Materials and methods:Human HCC SMMC-7721 and Hep3B cells were co-incubated with NK92-MI cells,berberine(60 or 80 mmol/L),or their combination for 36 h.To evaluate the killing effect of NK92-MI cells on HCC cells,the release of lactate dehydrogenase(LDH)in HCC cells was measured.The anti-tumor effects of berberine,NK92-MI cells,and their combinations were evaluated by MTS,EdU,Tunel,and Western blot assays.A male BALB/c nude mouse subcutaneous tumor model was used to investigate the anti-HCC effect of berberine and NK92-MI cells in vivo.Results:The LDH assay showed that berberine enhanced the cytotoxicity of NK92-MI cells on HCC cells.The combination of berberine and NK92-MI cells demonstrated more obvious anti-HCC effect than did the berberine or NK92-MI single treatment in inhibiting cell proliferation and inducing apoptosis both in vitro and in vivo.Mechanistically,the expression of programmed cell death-ligand 1(PD-L1)in HCC cells was upregulated after co-culture with NK-92MI cells.PD-L1 expression was knocked down,thereby inhibiting the proliferation and promoting apoptosis of HCC cells,and inhibited by berberine that blocked the secretion of interferon gamma(IFN-g),thereby enhancing the anti-tumor effect of NK92-MI cells.Conclusions:Current data show that the immunomodulatory role of berberine is to enhance the cytotoxic effect of NK92-MI cells and inhibit tumor immune escape by reducing the expression of PD-L1.Our study provides a rationale for the clinical application of berberine in combination with NK cells for the treatment of HCC.
