A key to tackling the coronavirus disease 2019(COVID-19)pandemic is to understand how severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)manages to outsmart host antiviral defense mechanisms.Stress granules(S...A key to tackling the coronavirus disease 2019(COVID-19)pandemic is to understand how severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)manages to outsmart host antiviral defense mechanisms.Stress granules(SGs),which are assembled during viral infection and function to sequester host and viral m RNAs and proteins,are part of the antiviral responses.Here,we show that the SARS-Co V-2 nucleocapsid(N)protein,an RNA binding protein essential for viral production,interacted with RasGTPase-activating protein SH3-domain-binding protein(G3 BP)and disrupted SG assembly,both of which require intrinsically disordered region1(IDR1)in N protein.The N protein partitioned into SGs through liquid-liquid phase separation with G3 BP,and blocked the interaction of G3 BP1 with other SG-related proteins.Moreover,the N protein domains important for phase separation with G3 BP and SG disassembly were required for SARS-Co V-2 viral production.We propose that N protein-mediated SG disassembly is crucial for SARS-Co V-2 production.展开更多
The establishment of self-recording precipitation observation systems in China began in 1951,and strips of self-recording precipitation graph paper have been archived since then.More than 9 million sheets of self-reco...The establishment of self-recording precipitation observation systems in China began in 1951,and strips of self-recording precipitation graph paper have been archived since then.More than 9 million sheets of self-recording graph paper from 2253 stations in 31 provinces have been digitized by using image scanning and curve extraction technology.Format specification and quality control have been applied to the digitized data,and the China Surface Self-Recording Per-Minute Precipitation Dataset(V1.0)has been developed.The integrity and accuracy of this dataset are evaluated.This is the first attempt in China to establish a per-minute precipitation dataset that covers the period from1951 to present.Preliminary evaluation reveals that the station density is high and the data continuity is good in most areas of China.However,the integrity of stations in some areas of western China is relatively poor.The availability rate and accuracy rate in summer are higher than 99%at most stations,with the overall availability and accuracy rates reaching as much as 99.42%and 99.22%,respectively.展开更多
基金supported by the National Natural Science Foundation of China(81830004,31970755,and 31970173)the Local Grant(608285568031)。
文摘A key to tackling the coronavirus disease 2019(COVID-19)pandemic is to understand how severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)manages to outsmart host antiviral defense mechanisms.Stress granules(SGs),which are assembled during viral infection and function to sequester host and viral m RNAs and proteins,are part of the antiviral responses.Here,we show that the SARS-Co V-2 nucleocapsid(N)protein,an RNA binding protein essential for viral production,interacted with RasGTPase-activating protein SH3-domain-binding protein(G3 BP)and disrupted SG assembly,both of which require intrinsically disordered region1(IDR1)in N protein.The N protein partitioned into SGs through liquid-liquid phase separation with G3 BP,and blocked the interaction of G3 BP1 with other SG-related proteins.Moreover,the N protein domains important for phase separation with G3 BP and SG disassembly were required for SARS-Co V-2 viral production.We propose that N protein-mediated SG disassembly is crucial for SARS-Co V-2 production.
基金Supported by the National Key Research and Development Program of China(2016YFA0600301 and 2016YFA0600302).
文摘The establishment of self-recording precipitation observation systems in China began in 1951,and strips of self-recording precipitation graph paper have been archived since then.More than 9 million sheets of self-recording graph paper from 2253 stations in 31 provinces have been digitized by using image scanning and curve extraction technology.Format specification and quality control have been applied to the digitized data,and the China Surface Self-Recording Per-Minute Precipitation Dataset(V1.0)has been developed.The integrity and accuracy of this dataset are evaluated.This is the first attempt in China to establish a per-minute precipitation dataset that covers the period from1951 to present.Preliminary evaluation reveals that the station density is high and the data continuity is good in most areas of China.However,the integrity of stations in some areas of western China is relatively poor.The availability rate and accuracy rate in summer are higher than 99%at most stations,with the overall availability and accuracy rates reaching as much as 99.42%and 99.22%,respectively.
