Drought stress impairs plant growth and other physiological functions. MeHDZ14, a homeodomainleucine zipper I transcription factor, is strongly induced by drought stress in various cassava cultivars.However, the role ...Drought stress impairs plant growth and other physiological functions. MeHDZ14, a homeodomainleucine zipper I transcription factor, is strongly induced by drought stress in various cassava cultivars.However, the role of MeHDZ14 in cassava growth regulation has remained unclear. Here we report that MeHDZ14 affected plant height, such that a dwarf phenotype and altered internode elongation were observed in transgenic cassava lines. MeHDZ14 was found to negatively regulate the biosynthesis of lignin. Its overexpression resulted in abaxially rolled leaves. The morphogenesis of leaf epidermal cells was inhibited by overexpression of MeHDZ14, with decreased auxin and gibberellin and increased cytokinin contents. MeHDZ14 was found to regulate many drought-responsive genes, including genes involved in cell wall synthesis and expansion. MeHDZ14 bound to the promoter of caffeic acid 3-Omethyltransferase 1(MeCOMT1), acting as a transcriptional repressor of genes involved in cell wall development. MeHDZ14 appears to act as a negative regulator of internode elongation and epidermal cell morphogenesis during cassava leaf development.展开更多
Chilling-induced accumulation of reactive oxygen species(ROS) is harmful to plants,which usually produce anthocyanins to scavenge ROS as protection from chilling stress.As a tropical crop,cassava is hypersensitive to ...Chilling-induced accumulation of reactive oxygen species(ROS) is harmful to plants,which usually produce anthocyanins to scavenge ROS as protection from chilling stress.As a tropical crop,cassava is hypersensitive to chilling,but the biochemical basis of this hypersensitivity remains unclear.We previously generated Me MYB2-RNAi transgenic cassava with increased chilling tolerance.Here we report that Me MYB2-RNAi transgenic cassava accumulated less ROS but more cyanidin-3-O-glucoside than the wild type under early chilling stress.Under this stress,the anthocyanin biosynthesis pathway was more active in Me MYB2-RNAi lines than in the wild type,and several genes involved in the pathway,including Me TT8,were up-regulated by Me MYB2-RNAi in the transgenic cassava.Me MYB2 bound to the Me TT8 promoter and blocked its expression under both normal and chilling conditions,thereby inhibiting anthocyanin accumulation.Me TT8 was shown to bind to the promoter of Dihydroflavonol 4-reductase(Me DFR-2)and increased Me DFR-2 expression.Me MYB2 appears to act as an inhibitor of chilling-induced anthocyanin accumulation during the rapid response of cassava to chilling stress.展开更多
Objective:To construct a nomogram based on the albumin-bilirubin(ALBI)grade to provide prognostic value for hepatitis C virus(HCV)-related hepatocellular carcinoma(HCC)patients who underwent ultrasound-guided percutan...Objective:To construct a nomogram based on the albumin-bilirubin(ALBI)grade to provide prognostic value for hepatitis C virus(HCV)-related hepatocellular carcinoma(HCC)patients who underwent ultrasound-guided percutaneous microwave ablation(US-PMWA).Methods:From April 2005 to January 2018,183 treatment-naIve patients with 251 HCV-related HCCs according to the Milan criteria received US-PMWA subsequently.The overall survival(OS)and recurrence-free survival(RFS)were compared between groups classified by ALBI grade.Cox proportional hazard regression model based on risk factors for survival and recurrence was used to construct the nomogram.Results:The cumulative OS rates at 1-,3-,5-and 10-year were 97.7%,73.6%,54.5%and 34.5%,respectively.Stratified according to ALBI grade,the 1-,3-,and 5-year OS in the ALBI grade 1 group and grade 2 group were 99.2%,92.4%,77.9% and 97.7%,52.3%,38.6%,respectively,with significant statistical difference(P<0.001).No significant statistical difference was detected in the1-,3-,and 5-year RFS rates in the ALBI grade 1 group and grade 2 group(P=0.220).The major complication rate was 1.6%.Multivariate analysis results showed age,α-fetoprotein level,tumor number,platelet count,location,Child-Turcotte-Pugh(CTP)and ALBI grade were associated with OS,which generated the nomograms.Internal validation with 1000 bootstrapped sample sets had good concordance index of 0.769(95%CI 0.699-0.839)in OS.Conclusions:This nomogram based on ALBI grade was a visualization risk model,which could provide personalized prediction of long-term outcomes for HCV-related HCC patients after US-PMWA.展开更多
Objective:To explore the association between cholecystectomy and the prognostic outcomes of patients with hepatocellular carcinoma(H CC)who underwent microwave ablation(MWA).Methods:Patients with HCC(«=921)who un...Objective:To explore the association between cholecystectomy and the prognostic outcomes of patients with hepatocellular carcinoma(H CC)who underwent microwave ablation(MWA).Methods:Patients with HCC(«=921)who underwent MWA were included and divided into cholecystectomy(n=114)and non-cholecystectomy groups(n=807).After propensity score matching(PSM)at a 1:2 ratio,overall survival(OS)and disease-free survival(DFS)rates were analyzed to compare prognostic outcomes between the cholecystectomy(«=114)and non-cholecystectomy groups(n=228).Univariate and multivariate Cox analyses were performed to assess potential risk factors for OS and DFS.Major complications were also compared between the groups.Results:After matching,no significant differences between groups were observed in baseline characteristics.The 1-,3-,and 5-year OS rates were 96.5%,82.1%,and 67.1%in the cholecystectomy group,and 97.4%,85.2%,and 74.4%in the non-cholecystectomy group(P=0.396);the 1-,3-,and 5-year DFS rates were 58.4%,34.5%,and 26.6%in the cholecystectomy group,and 73.6%,44.7%,and 32.2%in the non-cholecystectomy group(P=0.026),respectively.The intrahepatic distant recurrence rate in the cholecystectomy group was significantly higher than that in the non-cholecystectomy group(P=0.026),and the local tumor recurrence and extrahepatic recurrence rates did not significantly differ between the groups(P=0.609 and P=0.879).Multivariate analysis revealed that cholecystectomy(HR=1.364,95%Cl 1.023-1.819,P=0.035),number of tumors(2 vs.1:HR=2.744,95%Cl 1.925-3.912,P<0.001;3 vs.1:HR=3.411,95%Cl 2.021-5.759,P<0.001),and y-GT levels(HR=1.003,95%Cl 1.000-1.006,P<0.024)were independent risk factors for DFS.The best y-GT level cut-off value for predicting median DFS was 39.6 U/L(area under the curve=0.600,P<0.05).A positive correlation was observed between cholecystectomy and y-GT level(r=0.108,95%Cl-0.001-0.214,P=0.047).Subgroup analysis showed that the DFS rates were significantly higher in the non-cholecystectomy group than the cholecystectomy group when Y-GT>39.6 U/L(i3=0.044).The 5-,10-,15-,20-,and 25-year recurrence rates from the time of cholecystectomy were 2.63%,21.93%,42.11%,58.77%,and 65.79%,respectively.A significant positive correlation was observed between cholecystectomy and the time from cholecystectomy to recurrence(r=0.205,95%Cl 0.016-0.379,P=0.029).There were no significant differences in complications between groups(P=0.685).Conclusions:Patients with HCC who underwent cholecystectomy were more likely to develop intrahepatic distant recurrence after MWA,an outcome probably associated with increased y-GT levels.Moreover,the recurrence rates increased with time.展开更多
Small RNAs(sRNAs)are essential regulatory molecules,and there are three major sRNA classes in plants:microRNAs(miRNAs),phased small interfering RNAs(phased siRNAs or phasiRNAs),and heterochromatic siRNAs(hc-siRNAs).Ex...Small RNAs(sRNAs)are essential regulatory molecules,and there are three major sRNA classes in plants:microRNAs(miRNAs),phased small interfering RNAs(phased siRNAs or phasiRNAs),and heterochromatic siRNAs(hc-siRNAs).Excluding miRNAs,the other two classes are not well annotated or available in public databases for most sequenced plant genomes.We performed a comprehensive sRNA annotation of 143 plant species that have fully sequenced genomes and next-generation sequencing sRNA data publicly available.The results are available via an online repository called sRNAanno(www.plantsRNAs.org).Compared with other public plant sRNA databases,we obtained was much more miRNA annotations,which are more complete and reliable because of the consistent and highly stringent criteria used in our miRNA annotations.sRNAanno also provides free access to genomic information for>22,721 PHAS loci and>22 million hc-siRNA loci annotated from these 143 plant species.Both miRNA and PHAS loci can be easily browsed to view their main features,and a collection of archetypal trans-acting siRNA 3(TAS3)genes were annotated separately for quick access.To facilitate the ease of sRNA annotation,sRNAanno provides free service for sRNA annotations to the community.In summary,the sRNAanno database is a great resource to facilitate genomic and genetic research on plant small RNAs.展开更多
Internet of Things(IoT),which provides the solution of connecting things and devices,has increasingly developed as vital tools to realize intelligent life.Generally,source-limited IoT sensors outsource their data to t...Internet of Things(IoT),which provides the solution of connecting things and devices,has increasingly developed as vital tools to realize intelligent life.Generally,source-limited IoT sensors outsource their data to the cloud,which arises the concerns that the transmission of IoT data is happening without appropriate consideration of the profound security challenges involved.Though encryption technology can guarantee the confidentiality of private data,it hinders the usability of data.Searchable encryption(SE)has been proposed to achieve secure data sharing and searching.However,most of existing SE schemes are designed under conventional hardness assumptions and may be vulnerable to the adversary with quantum computers.Moreover,the untrusted cloud server may perform an unfaithful search execution.To address these problems,in this paper,we propose the first verifiable identity-based keyword search(VIBKS)scheme from lattice.In particular,a lattice-based delegation algorithm is adopted to help the data user to verify both the correctness and the integrity of the search results.Besides,in order to reduce the communication overhead,we refer to the identity-based mechanism.We conduct rigorous proof to demonstrate that the proposed VIBKS scheme is ciphertext indistinguishable secure against the semi-honestbut-curious adversary.In addition,we give the detailed computation and communication complexity of our VIBKS and conduct a series of experiments to validate its efficiency performance.展开更多
To save the local storage,users store the data on the cloud server who offers convenient internet services.To guarantee the data privacy,users encrypt the data before uploading them into the cloud server.Since encrypt...To save the local storage,users store the data on the cloud server who offers convenient internet services.To guarantee the data privacy,users encrypt the data before uploading them into the cloud server.Since encryption can reduce the data availability,public-key encryption with keyword search(PEKS)is developed to achieve the retrieval of the encrypted data without decrypting them.However,most PEKS schemes cannot resist quantum computing attack,because the corresponding hardness assumptions are some number theory problems that can be solved efficiently under quantum computers.Besides,the traditional PEKS schemes have an inherent security issue that they cannot resist inside keywords guessing attack(KGA).In this attack,a malicious server can guess the keywords encapsulated in the search token by computing the ciphertext of keywords exhaustively and performing the test between the token and the ciphertext of keywords.In the paper,we propose a lattice-based PEKS scheme that can resist quantum computing attacks.To resist inside KGA,this scheme adopts a lattice-based signature technique into the encryption of keywords to prevent the malicious server from forging a valid ciphertext.Finally,some simulation experiments are conducted to demonstrate the performance of the proposed scheme and some comparison results are further shown with respect to other searchable schemes.展开更多
Searchable encryption technology makes it convenient to search encrypted data with keywords for people.A data owner shared his data with other users on the cloud server.For security,it is necessary for him to build a ...Searchable encryption technology makes it convenient to search encrypted data with keywords for people.A data owner shared his data with other users on the cloud server.For security,it is necessary for him to build a fine-grained and flexible access control mechanism.The main idea of this paper is to let the owner classify his data and then authorizes others according to categories.The cloud server maintains a permission matrix,which will be used to verify whether a trapdoor is valid or not.In this way we can achieve access control and narrow the search range at the same time.We prove that our scheme can achieve index and trapdoor indistinguishability under chosen keywords attack security in the random oracles.展开更多
Objective:Thermal ablation poses challenges in the surgical resection(SR)of small hepatocellular carcinoma(HCC),and its therapeutic outcomes for larger lesions remain debated.Methods:This retrospective study evaluated...Objective:Thermal ablation poses challenges in the surgical resection(SR)of small hepatocellular carcinoma(HCC),and its therapeutic outcomes for larger lesions remain debated.Methods:This retrospective study evaluated 729 patients with HCC meeting the Milan criteria,who were treated with curative SR or microwave ablation(MWA)between 2008 and 2014.Overall survival(OS),cancer-specific survival(CSS),disease-free survival(DFS),and local tumor progression(LTP)were compared after propensity score matching(PSM).Co-variates associated with OS,CSS,LTP,and DFS were identified.The risk of death and tumor progression were compared.Results:During the median follow-up of 78.6 months,253 patients were included in each group after PSM.For tumors≤3.0 cm and 3.1–4.0 cm,MWA achieved comparable results in terms of OS,CSS,DFS,and LTP.For tumors 4.1–5.0 cm,MWA had lower OS,CSS,and DFS rates(all P<0.05)than SR.Higher LTP rates were observed in the MWA group for tumors 4.1–5.0 cm,although the difference was not significant(P=0.18).Complication rates(P=0.41)were similar,but MWA led to less estimated blood loss(P<0.01)and shorter postoperative hospitalization times(P<0.01).Conclusions:MWA achieved comparable long-term oncologic outcomes with SR for≤4 cm HCC,with lower complication rates and faster recovery.展开更多
We use a large sample of gamma-ray narrow-line Seyfert 1 galaxies(γ-NLS1 s) to study the jet formation mechanisms. We find that the jet power of γ-NLS1 s is lower than the maximum jet power of the Blandford–Payne(B...We use a large sample of gamma-ray narrow-line Seyfert 1 galaxies(γ-NLS1 s) to study the jet formation mechanisms. We find that the jet power of γ-NLS1 s is lower than the maximum jet power of the Blandford–Payne(BP) mechanism. At the same time, we find that there is a significant correlation between jet power and accretion disk luminosity. Moreover, the contribution rates of the accretion to the jet power are larger than that of black hole mass to jet power. These results further suggest that the jet of γ NLS1 s is mainly produced by the BP mechanism.展开更多
This paper proposes the first code-based quantum immune sequential aggregate signature(SAS)scheme and proves the security of the proposed scheme in the random oracle model.Aggregate signature(AS)schemes and sequential...This paper proposes the first code-based quantum immune sequential aggregate signature(SAS)scheme and proves the security of the proposed scheme in the random oracle model.Aggregate signature(AS)schemes and sequential aggregate signature schemes allow a group of potential signers to sign different messages respectively,and all the signatures of those users on those messages can be aggregated into a single signature such that the size of the aggregate signature is much smaller than the total size of all individual signatures.Because of the aggregation of many signatures into a single short signature,AS and SAS schemes can reduce bandwidth and save storage;moreover,when a SAS is verified,not only the valid but also the order in which each signer signed can be verified.AS and SAS schemes can be applied to traffic control,banking transaction and military applications.Most of the existing AS and SAS schemes are based either on pairing or Rivest-Shamir-Adleman(RSA),and hence,can be broken by Shor’s quantum algorithm for Integer Factoring Problem(IFP)and Discrete Logarithm Problem(DLP).There are no quantum algorithms to solve syndrome decoding problems.Hence,code-based cryptography is seen as one of the promising candidates for post-quantum cryptography.This paper shows how to construct quantum immune sequential aggregate signatures based on coding theory.Specifically,we construct our scheme with the first code based signature scheme proposed by Courtois,Finiasz and Sendrier(CFS).Compared to the CFS signature scheme without aggregation,the proposed sequential aggregate signature scheme can save about 90%storage when the number of signers is asymptotically large.展开更多
In this paper, through understanding the basic situation of three villages at different stages of life cycle development about tourism, using the method of grey correlation analysis from the quantitative aspects, the ...In this paper, through understanding the basic situation of three villages at different stages of life cycle development about tourism, using the method of grey correlation analysis from the quantitative aspects, the results show that impact assessment on rural tourism in different stages is different [1]. The impact on tourism stable stage is the most;tourism decline stage is relatively weak;tourism development stage is the weakest.展开更多
In this paper, a new topological approach for studying an integer sequence constructed from Logistic mapping is proposed. By evenly segmenting [0,1]?into N non-overlapping subintervals which is marked as , representin...In this paper, a new topological approach for studying an integer sequence constructed from Logistic mapping is proposed. By evenly segmenting [0,1]?into N non-overlapping subintervals which is marked as , representing the nodes identities, a network is constructed for analysis. Wherein the undirected edges symbolize their relation of adjacency in an integer sequence obtained from the Logistic mapping and the top integral function. By observation, we find that nodes’ degree changes with different values of??instead of the initial value—X0, and the degree distribution presents the characteristics of scale free network, presenting power law distribution. The results presented in this paper provide some insight into degree distribution of the network constructed from integer sequence that may help better understanding of the nature of Logistic mapping.展开更多
We carry out a field survey on the implementation of the"Whole Village Advancement"poverty alleviation model in Lisizhuang Village,Shunping County,Hebei Province.Based on the"Whole Village Advancement&q...We carry out a field survey on the implementation of the"Whole Village Advancement"poverty alleviation model in Lisizhuang Village,Shunping County,Hebei Province.Based on the"Whole Village Advancement"poverty alleviation model,we put forth the following recommendations:(i)Oriented by the market,fostering the function of"blood-refreshing";(ii)Strengthening the new ideas of using projects to attract investment;(iii)Implementing the new strategy of"driven by the able person";(iv)Improving the farmers'quality;(v)Adhering to the diversification of financing channels.展开更多
In this paper, taking three ethnic minority areas as an example, through a questionnaire to obtain relevant data, according to the characteristics of the ethnic minority areas, using the factor analysis method, we ana...In this paper, taking three ethnic minority areas as an example, through a questionnaire to obtain relevant data, according to the characteristics of the ethnic minority areas, using the factor analysis method, we analyse the tourism in ethnic areas of country life with various effects. We summarize the pros and cons, avoid or minimize the adverse impacts within the maximum limits, mitigate the conflicts between the value of resource and the development, find a balance between tourism development and rural construction, realize win-win tourism development and rural construction.展开更多
We used a questionnaire to obtain data about 664 university students’ amative behavior in a campus. Then we studied dissemination rules of university students’ amative behavior on campus social network. We found the...We used a questionnaire to obtain data about 664 university students’ amative behavior in a campus. Then we studied dissemination rules of university students’ amative behavior on campus social network. We found the amative behavior changes over time by focusing on the analysis of love group’s influence to single people and single group’s affected degree. Meanwhile, we compared the influence of single and multiple social relationships to the spread of amative behavior, and the result shows diversity of social relationships is a significant effect factor in spreading process.展开更多
Cassava(Manihot esculenta Crantz)is a root crop significant in food security and various bio-industrial applications such as animal feed,modified starch,and biofuels.Drought and cold stress are two major factors limit...Cassava(Manihot esculenta Crantz)is a root crop significant in food security and various bio-industrial applications such as animal feed,modified starch,and biofuels.Drought and cold stress are two major factors limiting cassava production qualitatively and quantitatively,for which plants have evolved mechanisms to overcome the impact of these two stressors.In recent years,significant progress has been achieved in understanding the response mechanism of cassava plants to stress signals to tolerate the above stresses.In this review,core stress-signaling pathways,including transcription factor(TF)-related regulatory networks,plant hormone signaling,reactive oxygen species(ROS)scavenging,and non-coding RNA(ncRNA)and alternative splicing(AS)that modify gene expression levels in response to drought and/or cold stress in cassava,are summarized.Understanding these stress signaling and responses will increase our ability to improve the crops tolerance to multiple stresses for agricultural sustainability and food security for the growing world population.展开更多
The theoretical model suggests that relativistic jets of active galactic nuclei(AGNs)rely on the black hole spin and/or accretion.We study the relationship between jet,accretion,and spin using supermassive black hole ...The theoretical model suggests that relativistic jets of active galactic nuclei(AGNs)rely on the black hole spin and/or accretion.We study the relationship between jet,accretion,and spin using supermassive black hole samples with reliable spin of black holes.Our results are as follows:(1)There is a weak correlation between radio luminosity and the spin of the black hole for our sample,which may imply that the jet of the supermassive black hole in our sample depends on the other physical parameters besides black hole spins,such as accretion disk luminosity.(2)The jet power of a supermassive black hole can be explained by the hybrid model with magnetic field of corona.(3)There is a significant correlation between radio-loudness and black hole spin for our sample.These sources with high radio-loudness tend to have high black hole spins.These results provide observational evidence that the black hole spin may explain the bimodal phenomena of radio-loud and radio-quiet AGNs.展开更多
The abnormal activation of CD4^(+)CD45RO+memory T(Tm)cells plays an important role in the pathogenesis of rheumatoid arthritis(RA).Previous studies have shown that CD147 participates in T-cell activation.However,it re...The abnormal activation of CD4^(+)CD45RO+memory T(Tm)cells plays an important role in the pathogenesis of rheumatoid arthritis(RA).Previous studies have shown that CD147 participates in T-cell activation.However,it remains unclear whether CD147 is involved in abnormal Tm-cell activation in RA patients.In this study,we demonstrated that CD147 was predominantly upregulated in Tm cells derived from RA patients.The anti-CD147 mAb 5A12 specifically inhibited Tm-cell activation and proliferation and further restrained osteoclastogenesis.Using a structural–functional approach,we depicted the interface between 5A12 and CD147.This allowed us to identify two critical residues,Lys63 and Asp65,as potential targets for RA treatment,as the double mutation K63A/D65A inhibited Tm-cell activation,mimicking the neutralization by 5A12.This study provides not only a theoretical basis for a“CD147-Tm/Osteoclast-RA chain”for the potential prevention and treatment of RA or other T-cell-mediated autoimmune diseases but also a new target for related drug design and development.展开更多
基金supported by the China Agriculture Research System (CARS11-HNCX)the Major Science and Technology Plan of Hainan Province (ZDKJ2021012)+3 种基金the Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (1630052022008)the National Key Research and Development Program of China (2018YFD1000501)the National Natural Science Foundation of China (31501378)the Hainan Yazhou Bay Seed Lab (B21HJ0303)。
文摘Drought stress impairs plant growth and other physiological functions. MeHDZ14, a homeodomainleucine zipper I transcription factor, is strongly induced by drought stress in various cassava cultivars.However, the role of MeHDZ14 in cassava growth regulation has remained unclear. Here we report that MeHDZ14 affected plant height, such that a dwarf phenotype and altered internode elongation were observed in transgenic cassava lines. MeHDZ14 was found to negatively regulate the biosynthesis of lignin. Its overexpression resulted in abaxially rolled leaves. The morphogenesis of leaf epidermal cells was inhibited by overexpression of MeHDZ14, with decreased auxin and gibberellin and increased cytokinin contents. MeHDZ14 was found to regulate many drought-responsive genes, including genes involved in cell wall synthesis and expansion. MeHDZ14 bound to the promoter of caffeic acid 3-Omethyltransferase 1(MeCOMT1), acting as a transcriptional repressor of genes involved in cell wall development. MeHDZ14 appears to act as a negative regulator of internode elongation and epidermal cell morphogenesis during cassava leaf development.
基金supported by the Hainan Provincial Natural Science Foundation of China (322RC798)the National Key Research and Development Program of China (2018YFD1000501)+1 种基金the Major Science and Technology Plan of Hainan Province (ZDKJ2021012)the China Agriculture Research System (CARS11-HNCX)。
文摘Chilling-induced accumulation of reactive oxygen species(ROS) is harmful to plants,which usually produce anthocyanins to scavenge ROS as protection from chilling stress.As a tropical crop,cassava is hypersensitive to chilling,but the biochemical basis of this hypersensitivity remains unclear.We previously generated Me MYB2-RNAi transgenic cassava with increased chilling tolerance.Here we report that Me MYB2-RNAi transgenic cassava accumulated less ROS but more cyanidin-3-O-glucoside than the wild type under early chilling stress.Under this stress,the anthocyanin biosynthesis pathway was more active in Me MYB2-RNAi lines than in the wild type,and several genes involved in the pathway,including Me TT8,were up-regulated by Me MYB2-RNAi in the transgenic cassava.Me MYB2 bound to the Me TT8 promoter and blocked its expression under both normal and chilling conditions,thereby inhibiting anthocyanin accumulation.Me TT8 was shown to bind to the promoter of Dihydroflavonol 4-reductase(Me DFR-2)and increased Me DFR-2 expression.Me MYB2 appears to act as an inhibitor of chilling-induced anthocyanin accumulation during the rapid response of cassava to chilling stress.
基金supported by the National Key R&D Program of China (Grant No. 2017YFC0112000)the National Natural Science Foundation of China (Grants No. 81627803, 91859201, and 81871374)the State Key Project on Infectious Disease of China (Grant No. 2018ZX10723204)
文摘Objective:To construct a nomogram based on the albumin-bilirubin(ALBI)grade to provide prognostic value for hepatitis C virus(HCV)-related hepatocellular carcinoma(HCC)patients who underwent ultrasound-guided percutaneous microwave ablation(US-PMWA).Methods:From April 2005 to January 2018,183 treatment-naIve patients with 251 HCV-related HCCs according to the Milan criteria received US-PMWA subsequently.The overall survival(OS)and recurrence-free survival(RFS)were compared between groups classified by ALBI grade.Cox proportional hazard regression model based on risk factors for survival and recurrence was used to construct the nomogram.Results:The cumulative OS rates at 1-,3-,5-and 10-year were 97.7%,73.6%,54.5%and 34.5%,respectively.Stratified according to ALBI grade,the 1-,3-,and 5-year OS in the ALBI grade 1 group and grade 2 group were 99.2%,92.4%,77.9% and 97.7%,52.3%,38.6%,respectively,with significant statistical difference(P<0.001).No significant statistical difference was detected in the1-,3-,and 5-year RFS rates in the ALBI grade 1 group and grade 2 group(P=0.220).The major complication rate was 1.6%.Multivariate analysis results showed age,α-fetoprotein level,tumor number,platelet count,location,Child-Turcotte-Pugh(CTP)and ALBI grade were associated with OS,which generated the nomograms.Internal validation with 1000 bootstrapped sample sets had good concordance index of 0.769(95%CI 0.699-0.839)in OS.Conclusions:This nomogram based on ALBI grade was a visualization risk model,which could provide personalized prediction of long-term outcomes for HCV-related HCC patients after US-PMWA.
基金the National Key R&D Program of China(Grant No.2017 YFC0112000)the National Natural Science Foundation of China(Grant Nos.81627803 and 91859201).
文摘Objective:To explore the association between cholecystectomy and the prognostic outcomes of patients with hepatocellular carcinoma(H CC)who underwent microwave ablation(MWA).Methods:Patients with HCC(«=921)who underwent MWA were included and divided into cholecystectomy(n=114)and non-cholecystectomy groups(n=807).After propensity score matching(PSM)at a 1:2 ratio,overall survival(OS)and disease-free survival(DFS)rates were analyzed to compare prognostic outcomes between the cholecystectomy(«=114)and non-cholecystectomy groups(n=228).Univariate and multivariate Cox analyses were performed to assess potential risk factors for OS and DFS.Major complications were also compared between the groups.Results:After matching,no significant differences between groups were observed in baseline characteristics.The 1-,3-,and 5-year OS rates were 96.5%,82.1%,and 67.1%in the cholecystectomy group,and 97.4%,85.2%,and 74.4%in the non-cholecystectomy group(P=0.396);the 1-,3-,and 5-year DFS rates were 58.4%,34.5%,and 26.6%in the cholecystectomy group,and 73.6%,44.7%,and 32.2%in the non-cholecystectomy group(P=0.026),respectively.The intrahepatic distant recurrence rate in the cholecystectomy group was significantly higher than that in the non-cholecystectomy group(P=0.026),and the local tumor recurrence and extrahepatic recurrence rates did not significantly differ between the groups(P=0.609 and P=0.879).Multivariate analysis revealed that cholecystectomy(HR=1.364,95%Cl 1.023-1.819,P=0.035),number of tumors(2 vs.1:HR=2.744,95%Cl 1.925-3.912,P<0.001;3 vs.1:HR=3.411,95%Cl 2.021-5.759,P<0.001),and y-GT levels(HR=1.003,95%Cl 1.000-1.006,P<0.024)were independent risk factors for DFS.The best y-GT level cut-off value for predicting median DFS was 39.6 U/L(area under the curve=0.600,P<0.05).A positive correlation was observed between cholecystectomy and y-GT level(r=0.108,95%Cl-0.001-0.214,P=0.047).Subgroup analysis showed that the DFS rates were significantly higher in the non-cholecystectomy group than the cholecystectomy group when Y-GT>39.6 U/L(i3=0.044).The 5-,10-,15-,20-,and 25-year recurrence rates from the time of cholecystectomy were 2.63%,21.93%,42.11%,58.77%,and 65.79%,respectively.A significant positive correlation was observed between cholecystectomy and the time from cholecystectomy to recurrence(r=0.205,95%Cl 0.016-0.379,P=0.029).There were no significant differences in complications between groups(P=0.685).Conclusions:Patients with HCC who underwent cholecystectomy were more likely to develop intrahepatic distant recurrence after MWA,an outcome probably associated with increased y-GT levels.Moreover,the recurrence rates increased with time.
基金the National Key Research and Developmental Program of China(2018YFD1000104)the National Natural Science Foundation of China(#31872063)+4 种基金the Special Support Program of Guangdong Province(2019TX05N193)the Guangzhou Science and Technology Key Project(201804020063)the Innovation Team Project of the Department of Education of Guangdong Province(2016KCXTD011)the Key Areas of Science and Technology Planning Project of Guangdong Province(2018B020202011)the Guangdong Innovative and Entrepreneurial Research Team Program(2016ZT06S172)。
文摘Small RNAs(sRNAs)are essential regulatory molecules,and there are three major sRNA classes in plants:microRNAs(miRNAs),phased small interfering RNAs(phased siRNAs or phasiRNAs),and heterochromatic siRNAs(hc-siRNAs).Excluding miRNAs,the other two classes are not well annotated or available in public databases for most sequenced plant genomes.We performed a comprehensive sRNA annotation of 143 plant species that have fully sequenced genomes and next-generation sequencing sRNA data publicly available.The results are available via an online repository called sRNAanno(www.plantsRNAs.org).Compared with other public plant sRNA databases,we obtained was much more miRNA annotations,which are more complete and reliable because of the consistent and highly stringent criteria used in our miRNA annotations.sRNAanno also provides free access to genomic information for>22,721 PHAS loci and>22 million hc-siRNA loci annotated from these 143 plant species.Both miRNA and PHAS loci can be easily browsed to view their main features,and a collection of archetypal trans-acting siRNA 3(TAS3)genes were annotated separately for quick access.To facilitate the ease of sRNA annotation,sRNAanno provides free service for sRNA annotations to the community.In summary,the sRNAanno database is a great resource to facilitate genomic and genetic research on plant small RNAs.
基金supported by the National Natural Science Foundation of China(No:62072240)the National Key Research and Development Program of China(No.2020YFB1804604).
文摘Internet of Things(IoT),which provides the solution of connecting things and devices,has increasingly developed as vital tools to realize intelligent life.Generally,source-limited IoT sensors outsource their data to the cloud,which arises the concerns that the transmission of IoT data is happening without appropriate consideration of the profound security challenges involved.Though encryption technology can guarantee the confidentiality of private data,it hinders the usability of data.Searchable encryption(SE)has been proposed to achieve secure data sharing and searching.However,most of existing SE schemes are designed under conventional hardness assumptions and may be vulnerable to the adversary with quantum computers.Moreover,the untrusted cloud server may perform an unfaithful search execution.To address these problems,in this paper,we propose the first verifiable identity-based keyword search(VIBKS)scheme from lattice.In particular,a lattice-based delegation algorithm is adopted to help the data user to verify both the correctness and the integrity of the search results.Besides,in order to reduce the communication overhead,we refer to the identity-based mechanism.We conduct rigorous proof to demonstrate that the proposed VIBKS scheme is ciphertext indistinguishable secure against the semi-honestbut-curious adversary.In addition,we give the detailed computation and communication complexity of our VIBKS and conduct a series of experiments to validate its efficiency performance.
基金The authors would like to thank the support from Fundamental Research Funds for the Central Universities(No.30918012204)The authors also gratefully acknowledge the helpful comments and suggestions of other researchers,which has improved the presentation.
文摘To save the local storage,users store the data on the cloud server who offers convenient internet services.To guarantee the data privacy,users encrypt the data before uploading them into the cloud server.Since encryption can reduce the data availability,public-key encryption with keyword search(PEKS)is developed to achieve the retrieval of the encrypted data without decrypting them.However,most PEKS schemes cannot resist quantum computing attack,because the corresponding hardness assumptions are some number theory problems that can be solved efficiently under quantum computers.Besides,the traditional PEKS schemes have an inherent security issue that they cannot resist inside keywords guessing attack(KGA).In this attack,a malicious server can guess the keywords encapsulated in the search token by computing the ciphertext of keywords exhaustively and performing the test between the token and the ciphertext of keywords.In the paper,we propose a lattice-based PEKS scheme that can resist quantum computing attacks.To resist inside KGA,this scheme adopts a lattice-based signature technique into the encryption of keywords to prevent the malicious server from forging a valid ciphertext.Finally,some simulation experiments are conducted to demonstrate the performance of the proposed scheme and some comparison results are further shown with respect to other searchable schemes.
基金This work is partially supported by the Fundamental Research Funds for the Central Universities(No.30918012204)。
文摘Searchable encryption technology makes it convenient to search encrypted data with keywords for people.A data owner shared his data with other users on the cloud server.For security,it is necessary for him to build a fine-grained and flexible access control mechanism.The main idea of this paper is to let the owner classify his data and then authorizes others according to categories.The cloud server maintains a permission matrix,which will be used to verify whether a trapdoor is valid or not.In this way we can achieve access control and narrow the search range at the same time.We prove that our scheme can achieve index and trapdoor indistinguishability under chosen keywords attack security in the random oracles.
基金supported by The National Key R&D Program of Ministry of Science and Technology of China(Grant No.2018ZX10723-204)The National Scientific Foundation Committee of China(Grant Nos.81801722,81971625,82030047,81627803,and 91859201)and The National Scientific Foundation Committee of Beijing(Grant No.JQ18021).
文摘Objective:Thermal ablation poses challenges in the surgical resection(SR)of small hepatocellular carcinoma(HCC),and its therapeutic outcomes for larger lesions remain debated.Methods:This retrospective study evaluated 729 patients with HCC meeting the Milan criteria,who were treated with curative SR or microwave ablation(MWA)between 2008 and 2014.Overall survival(OS),cancer-specific survival(CSS),disease-free survival(DFS),and local tumor progression(LTP)were compared after propensity score matching(PSM).Co-variates associated with OS,CSS,LTP,and DFS were identified.The risk of death and tumor progression were compared.Results:During the median follow-up of 78.6 months,253 patients were included in each group after PSM.For tumors≤3.0 cm and 3.1–4.0 cm,MWA achieved comparable results in terms of OS,CSS,DFS,and LTP.For tumors 4.1–5.0 cm,MWA had lower OS,CSS,and DFS rates(all P<0.05)than SR.Higher LTP rates were observed in the MWA group for tumors 4.1–5.0 cm,although the difference was not significant(P=0.18).Complication rates(P=0.41)were similar,but MWA led to less estimated blood loss(P<0.01)and shorter postoperative hospitalization times(P<0.01).Conclusions:MWA achieved comparable long-term oncologic outcomes with SR for≤4 cm HCC,with lower complication rates and faster recovery.
基金supported from the research project of Qujing Normal University (Grant No. 2105098001/094)supported by the youth of Yunnan Provincial Science and Technology Department (Grant Nos. 202101AU070146 and 2103010006)+3 种基金supported by the National Natural Science Foundation of China (NSFC, Grant Nos.11733001, 11733002 and 11773013)supported by the National Key Research and Development Program of China (Grant No. 2017YFA0402703)financial support from the National Natural Science Foundation of China (NSFC, Grant No. 12103022)the Special Basic Cooperative Research Programs of Yunnan Provincial Undergraduate Universities Association (No.202101BA070001-043)。
文摘We use a large sample of gamma-ray narrow-line Seyfert 1 galaxies(γ-NLS1 s) to study the jet formation mechanisms. We find that the jet power of γ-NLS1 s is lower than the maximum jet power of the Blandford–Payne(BP) mechanism. At the same time, we find that there is a significant correlation between jet power and accretion disk luminosity. Moreover, the contribution rates of the accretion to the jet power are larger than that of black hole mass to jet power. These results further suggest that the jet of γ NLS1 s is mainly produced by the BP mechanism.
基金This work was supported in part by the National Natural Science Foundation of China under Grant 62072240by the Natural Science Foundation of Jiangsu Province under Grant BK20210330by the National Key Research and Development Program of China under Grant 2020YFB1804604.
文摘This paper proposes the first code-based quantum immune sequential aggregate signature(SAS)scheme and proves the security of the proposed scheme in the random oracle model.Aggregate signature(AS)schemes and sequential aggregate signature schemes allow a group of potential signers to sign different messages respectively,and all the signatures of those users on those messages can be aggregated into a single signature such that the size of the aggregate signature is much smaller than the total size of all individual signatures.Because of the aggregation of many signatures into a single short signature,AS and SAS schemes can reduce bandwidth and save storage;moreover,when a SAS is verified,not only the valid but also the order in which each signer signed can be verified.AS and SAS schemes can be applied to traffic control,banking transaction and military applications.Most of the existing AS and SAS schemes are based either on pairing or Rivest-Shamir-Adleman(RSA),and hence,can be broken by Shor’s quantum algorithm for Integer Factoring Problem(IFP)and Discrete Logarithm Problem(DLP).There are no quantum algorithms to solve syndrome decoding problems.Hence,code-based cryptography is seen as one of the promising candidates for post-quantum cryptography.This paper shows how to construct quantum immune sequential aggregate signatures based on coding theory.Specifically,we construct our scheme with the first code based signature scheme proposed by Courtois,Finiasz and Sendrier(CFS).Compared to the CFS signature scheme without aggregation,the proposed sequential aggregate signature scheme can save about 90%storage when the number of signers is asymptotically large.
文摘In this paper, through understanding the basic situation of three villages at different stages of life cycle development about tourism, using the method of grey correlation analysis from the quantitative aspects, the results show that impact assessment on rural tourism in different stages is different [1]. The impact on tourism stable stage is the most;tourism decline stage is relatively weak;tourism development stage is the weakest.
文摘In this paper, a new topological approach for studying an integer sequence constructed from Logistic mapping is proposed. By evenly segmenting [0,1]?into N non-overlapping subintervals which is marked as , representing the nodes identities, a network is constructed for analysis. Wherein the undirected edges symbolize their relation of adjacency in an integer sequence obtained from the Logistic mapping and the top integral function. By observation, we find that nodes’ degree changes with different values of??instead of the initial value—X0, and the degree distribution presents the characteristics of scale free network, presenting power law distribution. The results presented in this paper provide some insight into degree distribution of the network constructed from integer sequence that may help better understanding of the nature of Logistic mapping.
基金Supported by National Social Science Foundation(11XRK004)National Soft Science Project(2010GXS5D271)Presidential Foundation of Tarim University(TDSKZD1102)
文摘We carry out a field survey on the implementation of the"Whole Village Advancement"poverty alleviation model in Lisizhuang Village,Shunping County,Hebei Province.Based on the"Whole Village Advancement"poverty alleviation model,we put forth the following recommendations:(i)Oriented by the market,fostering the function of"blood-refreshing";(ii)Strengthening the new ideas of using projects to attract investment;(iii)Implementing the new strategy of"driven by the able person";(iv)Improving the farmers'quality;(v)Adhering to the diversification of financing channels.
文摘In this paper, taking three ethnic minority areas as an example, through a questionnaire to obtain relevant data, according to the characteristics of the ethnic minority areas, using the factor analysis method, we analyse the tourism in ethnic areas of country life with various effects. We summarize the pros and cons, avoid or minimize the adverse impacts within the maximum limits, mitigate the conflicts between the value of resource and the development, find a balance between tourism development and rural construction, realize win-win tourism development and rural construction.
文摘We used a questionnaire to obtain data about 664 university students’ amative behavior in a campus. Then we studied dissemination rules of university students’ amative behavior on campus social network. We found the amative behavior changes over time by focusing on the analysis of love group’s influence to single people and single group’s affected degree. Meanwhile, we compared the influence of single and multiple social relationships to the spread of amative behavior, and the result shows diversity of social relationships is a significant effect factor in spreading process.
基金the Central Publicinterest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(1630052021026,1630052022008)the Major Science and Technology plan of Hainan Province(ZDKJ2021012)+1 种基金the National Key Research and Development Program of China(2018YFD1000500,2019YFD 1000500 and 2019YFD1001105)the Hainan Provincial Natural Science Foundation of China(320MS097).
文摘Cassava(Manihot esculenta Crantz)is a root crop significant in food security and various bio-industrial applications such as animal feed,modified starch,and biofuels.Drought and cold stress are two major factors limiting cassava production qualitatively and quantitatively,for which plants have evolved mechanisms to overcome the impact of these two stressors.In recent years,significant progress has been achieved in understanding the response mechanism of cassava plants to stress signals to tolerate the above stresses.In this review,core stress-signaling pathways,including transcription factor(TF)-related regulatory networks,plant hormone signaling,reactive oxygen species(ROS)scavenging,and non-coding RNA(ncRNA)and alternative splicing(AS)that modify gene expression levels in response to drought and/or cold stress in cassava,are summarized.Understanding these stress signaling and responses will increase our ability to improve the crops tolerance to multiple stresses for agricultural sustainability and food security for the growing world population.
基金financial support from the National Natural Science Foundation of China(NSFC,No.12203028)supported by the research project of Qujing Normal University(2105098001/094)+4 种基金supported by the youth project of Yunnan Provincial Science and Technology Department(202101AU070146,2103010006)funding for the training Program for talents in Xingdian,Yunnan Provincesupported by the NSFC(12121003,12192220,and 12192222)the science research grants from the China Manned Space Project with No.CMS-CSST-2021-A05supported by the NSFC(11733001,U2031201 and 12433004)。
文摘The theoretical model suggests that relativistic jets of active galactic nuclei(AGNs)rely on the black hole spin and/or accretion.We study the relationship between jet,accretion,and spin using supermassive black hole samples with reliable spin of black holes.Our results are as follows:(1)There is a weak correlation between radio luminosity and the spin of the black hole for our sample,which may imply that the jet of the supermassive black hole in our sample depends on the other physical parameters besides black hole spins,such as accretion disk luminosity.(2)The jet power of a supermassive black hole can be explained by the hybrid model with magnetic field of corona.(3)There is a significant correlation between radio-loudness and black hole spin for our sample.These sources with high radio-loudness tend to have high black hole spins.These results provide observational evidence that the black hole spin may explain the bimodal phenomena of radio-loud and radio-quiet AGNs.
基金supported by grant(2015CB553704)from the National Basic Research Program of China,grants(2013ZX09301301 and 2014ZX09508002-002)+3 种基金from the National Science and Technology Major Project of China,grant(2017YFC0909002)from the National Key Research Project of China,grant(31470792)from the National Natural Science Foundation of China and grant(XDB08030102)from the Strategic Priority Research Program of the Chinese Academy of Sciences.
文摘The abnormal activation of CD4^(+)CD45RO+memory T(Tm)cells plays an important role in the pathogenesis of rheumatoid arthritis(RA).Previous studies have shown that CD147 participates in T-cell activation.However,it remains unclear whether CD147 is involved in abnormal Tm-cell activation in RA patients.In this study,we demonstrated that CD147 was predominantly upregulated in Tm cells derived from RA patients.The anti-CD147 mAb 5A12 specifically inhibited Tm-cell activation and proliferation and further restrained osteoclastogenesis.Using a structural–functional approach,we depicted the interface between 5A12 and CD147.This allowed us to identify two critical residues,Lys63 and Asp65,as potential targets for RA treatment,as the double mutation K63A/D65A inhibited Tm-cell activation,mimicking the neutralization by 5A12.This study provides not only a theoretical basis for a“CD147-Tm/Osteoclast-RA chain”for the potential prevention and treatment of RA or other T-cell-mediated autoimmune diseases but also a new target for related drug design and development.
