Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease.Transplantation of midbrain dopaminergic progenitor cells is a p...Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease.Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s disease.However,transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation niche.Here,we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor cells.These beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion,effectively maintaining axonal integrity in vitro.Importantly,midbrain dopaminergic progenitor cell bead grafts showed increased survival and only mild immunoreactivity in vivo compared with suspended midbrain dopaminergic progenitor cell grafts.Overall,our findings show that these midbrain dopaminergic progenitor cell beads enhance the effectiveness of neuronal cell transplantation.展开更多
To date,few studies have reported on the folding mechanism of tandem G-quadruplexes in human telomeric DNA.Hence,the control of the biofunctions of G-quadruplex,which requires a thorough understanding of its dynamic b...To date,few studies have reported on the folding mechanism of tandem G-quadruplexes in human telomeric DNA.Hence,the control of the biofunctions of G-quadruplex,which requires a thorough understanding of its dynamic behavior,is limited.Here,we investigated the folding/unfolding behavior of human telomeric sequences with lengths over 10 kilonucleotide(knt)by circular dichroism(CD)spectroscopy,UV melting assay,and atomic force microscopy(AFM)-based single-molecule force spectroscopy.展开更多
基金supported by the National Key Research and Development Program of China,Nos.2017YFE0122900(to BH),2019YFA0110800(to WL),2019YFA0903802(to YW),2021YFA1101604(to LW),2018YFA0108502(to LF),and 2020YFA0804003(to JW)the National Natural Science Foundation of China,Nos.31621004(to WL,BH)and 31970821(to YW)+1 种基金CAS Project for Young Scientists in Basic Research,No.YSBR-041(to YW)Joint Funds of the National Natural Science Foundation of China,No.U21A20396(to BH)。
文摘Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease.Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s disease.However,transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation niche.Here,we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor cells.These beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion,effectively maintaining axonal integrity in vitro.Importantly,midbrain dopaminergic progenitor cell bead grafts showed increased survival and only mild immunoreactivity in vivo compared with suspended midbrain dopaminergic progenitor cell grafts.Overall,our findings show that these midbrain dopaminergic progenitor cell beads enhance the effectiveness of neuronal cell transplantation.
基金funded by the National Natural Science Foundation of China(nos.21827805 and 21525418).
文摘To date,few studies have reported on the folding mechanism of tandem G-quadruplexes in human telomeric DNA.Hence,the control of the biofunctions of G-quadruplex,which requires a thorough understanding of its dynamic behavior,is limited.Here,we investigated the folding/unfolding behavior of human telomeric sequences with lengths over 10 kilonucleotide(knt)by circular dichroism(CD)spectroscopy,UV melting assay,and atomic force microscopy(AFM)-based single-molecule force spectroscopy.