Thallium(Tl)compounds,highly toxic to biology,are usually released into flue gas during fossil/minerals combustion,and further distributed in water and soil.In this work,we fundamentally investigated the capture of ga...Thallium(Tl)compounds,highly toxic to biology,are usually released into flue gas during fossil/minerals combustion,and further distributed in water and soil.In this work,we fundamentally investigated the capture of gaseous Tl_(2)O by industrial V2O5-WO3/TiO_(2)catalyst under working condition in Tl-containing flue gas.Experimental and theoretical results indicated that the Tl_(2)O has significant electron-feeding capacity and easily donate electron to unoccupied orbitals of TiO_(2),leading to dismutation of Ti 2p and inartificial formation of p-n junction on TiO_(2)surface,which prompted Tl_(2)O selectively interacted with TiO_(2)in flue gas.Herein,we proposed and verified an effective way to capture gaseous Tl_(2)O,which offered almost the best choice to eliminate Tl emission from flue gas and expanded the function of the TiO_(2)-based catalyst.The formation of p-n junction on commercial V2O5-WO3/TiO_(2)catalyst under working condition was revealed for the first time,which can be a valuable reference for both heterocatalysis and electro/photocatalysis.展开更多
Improvement of photosynthetic efficiency is a major approach to increase crop yield potential.Previously,we cloned a gene encoding the chloroplast-located putative monooxygenase TCD5,which is essential in plastid deve...Improvement of photosynthetic efficiency is a major approach to increase crop yield potential.Previously,we cloned a gene encoding the chloroplast-located putative monooxygenase TCD5,which is essential in plastid development under low temperature in rice(Oryza sativa L.).In this study,the effects of TCD5 on the photosynthesis and the yields were investigated in rice.Two sets of genetic materials with three levels of TCD5 expression,including tcd5 mutant or TCD5 RNAi transgenic lines and TCD5 over-expression transgenic lines in Jiahua1 and Nipponbare backgrounds,were used in the field trails of multi-locations and multi-years.TCD5 positively affected the panicle number and the yield at dosage.Compared with the wild-types,the panicle numbers were 12.4%-14.6%less in tcd5 mutant and 8.3%-38.6%less in TCD5 RNAi lines,but 26.2%-61.8%more in TCD5 over-expression lines.The grain yields per plant were 9.1%-18.4%less in tcd5 mutant and 14.3%-56.7%less in TCD5 RNAi lines,but 6.9%-56.5%more in TCD5 over-expression lines.The measurements of net photosynthetic rate indicated that mutation or knock down of TCD5 decreased the net photosynthetic rate by 10.4%and 15.6%,respectively,while increasing it by 8.9%and 8.7%in the TCD5 over-expression lines in Jiahua1 and Nipponbare backgrounds,respectively.Accordingly,the measurements of chlorophyll fluorescence parameters showed that the electron transport rate and quantum yield decreased in tcd5 mutant or TCD5 RNAi lines but increased in TCD5 overexpression lines,both in Jiahua1 and Nipponbare backgrounds.IP-MS screening revealed that TCD5 interacts with 29 chloroplast proteins involved in chlorophyll synthesis,photo-reactions of the photosynthesis,carbon assimilation and metabolism,energy metabolism,redox balance,protein synthesis and transportation.Two TCD5 interacted proteins,D1 and FBA were effective targets for improving photosynthesis.These results suggest a potentially new strategy for increasing rice yield by enhancing photosynthesis.展开更多
Augmented-and mixed-reality technologies have pioneered the realization of real-time fusion and interactive projection for laparoscopic surgeries.Indocyanine green fluorescence imaging technology has enabled anatomica...Augmented-and mixed-reality technologies have pioneered the realization of real-time fusion and interactive projection for laparoscopic surgeries.Indocyanine green fluorescence imaging technology has enabled anatomical,functional,and radical hepatectomy through tumor identification and localization of target hepatic segments,driving a transformative shift in themanagement of hepatic surgical diseases,moving away from traditional,empirical diagnostic and treatment approaches toward digital,intelligent ones.The Hepatic Surgery Group of the Surgery Branch of the Chinese Medical Association,Digital Medicine Branch of the Chinese Medical Association,Digital Intelligent Surgery Committee of the Chinese Society of ResearchHospitals,and Liver Cancer Committee of the Chinese Medical Doctor Association organized the relevant experts in China to formulate this consensus.This consensus provides a comprehensive outline of the principles,advantages,processes,and key considerations associated with the application of augmented reality and mixed-reality technology combined with indocyanine green fluorescence imaging technology for hepatic segmental and subsegmental resection.The purpose is to streamline and standardize the application of these technologies.展开更多
Continuous deforming always leads to the performance degradation of a flexible triboelectric nanogenerator due to the Young’s modulus mismatch of different functional layers.In this work,we fabricated a fiber-shaped ...Continuous deforming always leads to the performance degradation of a flexible triboelectric nanogenerator due to the Young’s modulus mismatch of different functional layers.In this work,we fabricated a fiber-shaped stretchable and tailorable triboelectric nanogenerator(FST-TENG)based on the geometric construction of a steel wire as electrode and ingenious selection of silicone rubber as triboelectric layer.Owing to the great robustness and continuous conductivity,the FST-TENGs demonstrate high stability,stretchability,and even tailorability.For a single device with ~6 cm in length and ~3 mm in diameter,the open-circuit voltage of ~59.7 V,transferred charge of ~23.7 nC,short-circuit current of ~2.67 μA and average power of ~2.13 μW can be obtained at 2.5 Hz.By knitting several FST-TENGs to be a fabric or a bracelet,it enables to harvest human motion energy and then to drive a wearable electronic device.Finally,it can also be woven on dorsum of glove to monitor the movements of gesture,which can recognize every single finger,different bending angle,and numbers of bent finger by analyzing voltage signals.展开更多
Oxysophoridine, a new alkaloid extracted from Sophora alopecuroides L., has been shown to have a protective effect against ischemic brain damage. In this study, a focal cerebral ischemia/reperfusion injury model was e...Oxysophoridine, a new alkaloid extracted from Sophora alopecuroides L., has been shown to have a protective effect against ischemic brain damage. In this study, a focal cerebral ischemia/reperfusion injury model was established using middle cerebral artery occlusion in mice. Both 62.5, 125, and 250 mg/kg oxysophoridine, via intraperitoneal injection, and 6 mg/kg nimodipine, via intragastric administration, were administered daily for 7 days before modeling. After 24 hours of reperfusion, mice were tested for neurological deficit, cerebral infarct size was assessed and brain tissue was collected. Results showed that oxysophoridine at 125, 250 mg/kg and 6 mg/kg nimodipine could reduce neurological deficit scores, cerebral infarct size and brain water content in mice. These results provided evidence that oxysophoridine plays a protective role in cerebral ischemia/reperfusion injury. In addition, oxysophoridine at 62.5, 125, and 250 mg/kg and 6 mg/kg nimodipine increased adenosine-triphosphate content, and decreased malondialdehyde and nitric oxide content. These compounds enhanced the activities of glutathione-peroxidase, superoxide dismutase, catalase, and lactate dehydrogenase, and decreased the activity of nitric oxide synthase. Protein and mRNA expression levels of N-methyl-D-aspartate receptor subunit NR1 were markedly inhibited in the presence of 250 mg/kg oxysophoridine and 6 mg/kg nimodipine. Our experimental findings indicated that oxysophoridine has a neuroprotective effect against cerebral ischemia/reperfusion injury in mice, and that the effect may be due to its ability to inhibit oxidative stress and expression of the N-methyl-D-aspartate receptor subunit NR1.展开更多
Progress has been developed in harvesting lowfrequency and irregular blue energy using a triboelectric–electromagnetic hybrid generator in recent years. However,the design of the high-efficiency, mechanically durable...Progress has been developed in harvesting lowfrequency and irregular blue energy using a triboelectric–electromagnetic hybrid generator in recent years. However,the design of the high-efficiency, mechanically durable hybrid structure is still challenging. In this study, we report a fully packaged triboelectric–electromagnetic hybrid generator(TEHG), in which magnets were utilized as the trigger to drive contact–separation-mode triboelectric nanogenerators(CS-TENGs) and coupled with copper coils to operate rotary freestanding-mode electromagnetic generators(RF-EMGs). The magnet pairs that produce attraction were used to transfer the external mechanical energy to the CS-TENGs, and packaging of the CS-TENGpart was achieved to protect it from the ambient environment. Under a rotatory speed of 100 rpm, the CS-TENGs enabled the TEHG to deliver an output voltage, current,and average power of 315.8 V, 44.6 μA, and ~ 90.7 μW,and the output of the RF-EMGs was 0.59 V, 1.78 m A, and 79.6 μW, respectively. The cylinder-like structure made the TEHG more easily driven by water flow and demonstrated to work as a practical power source to charge commercial capacitors. It can charge a 33μF capacitor from 0 to 2.1 V in 84 s, and the stored energy in the capacitor can drive an electronic thermometer and form a self-powered water-temperature sensing system.展开更多
Photoelectrochemical(PEC) water splitting using solar energy has attracted great attention for generation of renewable hydrogen with less carbon footprint, while there are enormous challenges that still remain for imp...Photoelectrochemical(PEC) water splitting using solar energy has attracted great attention for generation of renewable hydrogen with less carbon footprint, while there are enormous challenges that still remain for improving solar energy water splitting efficiency, due to limited light harvesting, energy loss associated to fast recombination of photogenerated charge carriers, as well as electrode degradation. This overview focuses on the recent development about catalyst nanomaterials and nanostructures in different PEC water splitting systems. As photoanode, Au nanoparticle-decorated TiO_2 nanowire electrodes exhibited enhanced photoactivity in both the UV and the visible regions due to surface plasmon resonance of Au and showed the largest photocurrent generation of up to 710 nm. Pt/Cd S/CGSe electrodes were developed as photocathode. With the role of p–n heterojunction, the photoelectrode showed high stability and evolved hydrogen continuously for more than 10 days. Further, in the Z-scheme system(Bi_2S_3/TNA as photoanode and Pt/Si PVC as photocathode at the same time), a self-bias(open-circuit voltage Voc= 0.766 V) was formed between two photoelectrodes, which could facilitate photogenerated charge transfers and enhance the photoelectrochemical performance, and which might provide new hints for PEC water splitting. Meanwhile, the existing problems and prospective solutions have also been reviewed.展开更多
Stability of an ancient landslide in a reservoir area is analyzed by using centrifugal model tests, soil laboratory tests and numerical analysis. Special attention is paid to variation in water level, simulation of la...Stability of an ancient landslide in a reservoir area is analyzed by using centrifugal model tests, soil laboratory tests and numerical analysis. Special attention is paid to variation in water level, simulation of large-scale heterogeneous prototype slope, and strength reduction of sliding zone soils after slope sliding. The results of centrifugal model test show that reservoir impounding can reduce sliding resistance at the slope toe, followed by toe collapsing and front cracking of slope. Rapid drawdown can produce hydrodynamic pressure towards reservoir at the front of slope. Deformation is observed in the middle and upper slope, which reduces the slope stability further and forms the pull-typed landslide trend. Reinforcement of slope toe is effective for preventing the progressive failure. The results of laboratory test show that slope toe sliding will lead to the redistribution of soil density and moisture content, which will reduce the shear strength of soil in sliding zone, and the cohesion of immersed soil is reduced gradually and finally vanishes with time. The numerical results show that the strength reduction method used in finite element method (FEM) is very effective in capturing the progressive failure induced by reservoir water level fluctuations, and the evolution of failure surface derived from numerical simulation is very similar to that observed in centrifugal model test.展开更多
BACKGROUND: With developments of tissue engineering and genetic engineering, we aim to culture myoblasts, which are characterized by high purity, high quality and high production, for wide application in neural regene...BACKGROUND: With developments of tissue engineering and genetic engineering, we aim to culture myoblasts, which are characterized by high purity, high quality and high production, for wide application in neural regeneration researches. OBJECTIVE: To modify traditional dissociation method in order to obtain myoblasts, which are characterized by high purity, high quality and high production, and explore the biological properties under in vitro culture. DESIGN: Observational study. SETTING: Basic Institute of Academy of Military Medical Sciences of Chinese PLA. MATERIALS: Four neonatal Wistar rats of 5 days old, both genders and mean body mass of 10 g were selected in this study. The main reagents and devices were detailed as follows: DMEM medium (Gibco Company), fetus bovine serum (FBS, Hycolne Company), collagenase Ⅱ(Sigma Company), trypsin (Sigma Company), dispase Ⅱ (Sigma Company), desmin antibody (Fuzhou Maixin Company), antibody Ⅱ and ABC kit (Wuhan Boster Biotechnology Company), desk centrifuge (KUBATO, Japan), and inverted phase contrast microscope (LEICA DMIRB, Germany). METHODS: The experiment was carried out in the Basic Institute of Academy of Military Medical Sciences of Chinese PLA from June to October 2006. Neonatal rats were sacrificed under sterile condition to obtain skeletal muscles of limbs, which were washed with cold PBS (containing benzylpenicillin and estreptomicina), and muscular tissue was sheared into pieces. Then, those muscular pieces were added with mixed digestive enzyme (containing 2 g/L collagenase Ⅱ + 5 g/L dispase Ⅱ + 0.28 g/L CaCl2) as twice volume as pieces, dealt with mechanical pipetting for 5 minutes and cultured in CO2 incubator for 10 minutes. The operation was done for three times and the muscular pieces were digested for 45 minutes in total. Moreover, cells were suspended again in order to obtain myoblasts from skeletal muscle of neonatal rats. In addition, myoblasts were purified with differential attachment technique and enzyme digestion so as to observe morphological characteristics and growth, draw growth curve, analyze surface structure under scanning electron microscope, and evaluate with Desmin immunohistochemical staining. MAIN OUTCOME MEASURES: Morphological characteristics and growth of myoblasts cultured in vitro. RESULTS: ① Growth of myoblasts of skeletal muscle: Primary cells had well growth, mature and differentiation. The positive rate of Desmin was 94% and purification of cells was ideal. Growth curve of cells demonstrated that myoblasts which were characterized by high purification started proliferation plentiful through transient growth lag phase (about at one or two days after inoculation). If myoblasts were not dealt with any interventions, they might become sarcotubule gradually at 3–5 days after proliferative phase. During this period, myoblasts maintained a monocaryon-bipolarity state under inverted phase contrast microscope. Furthermore, the growth of cells was the strongest and reproductive activity was the most powerful. This suggested that myotube started to form; in addition, muscle fiber of contractility might form under a well culturing condition. ② Immunocytochemical stain with desmin antibody: Interzonal fiber of desmin from myoblasts showed strongly positive reaction. Positive staining existed in cytoplasm had a high nucleus-cytoplasm ratio. However, myoblasts showed negative or mildly positive reaction. CONCLUSION: It is ideal for modified multi-enzymatic digestion and double purification method to dissociate and purify myoblasts of skeletal muscle; meanwhile, these two methods are both the effective ways to provide convenient conditions to obtain seed cells for neural regeneration researches.展开更多
The objectives of this study are to present a detailed and comprehensive description of the osteology of Quasipaa robertingeri(Anura: Dicroglossidae), to provide osteological evidence for taxonomy, and to explore the ...The objectives of this study are to present a detailed and comprehensive description of the osteology of Quasipaa robertingeri(Anura: Dicroglossidae), to provide osteological evidence for taxonomy, and to explore the adaptive traits of Q. robertingeri. We comprehensively studied the osteology of 2 adult specimens(1 male 98A00351 and 1 female 98A0041) of Q. robertingeri using conventional methods. Our osteological examination of Q. robertingerishows that 1) the nasals are large and connect with one another medially; 2) the sphenethmoid is completely covered by the nasals and the frontopatietals, and thus it is not exposed dorsally; 3) the vomerine teeth are well-developed; 4) the anterior parts of the diapophyses of the presacral II protrude forward in the male, the same observation was made in the presacrals II and III in the female; 5) the pectoral girdle is firmisternal; 6) the omosternum is fan-shaped posteriorly, and almost equal to the length of the mesosternum; 7) the xiphisternum is W-shaped with a deep incision posteriorly; 8) the humerus of the male is more developed in the crista ventralis, the crista medialis, and the crista lateralis; 9) the metacarpal II of the male presents with a nuptial tuber; and 10) the distal phalanges of the digits are anchorshaped. Ultimately, the unique characteristics listed are most likely adaptations to the environment and amplexus of Q. robertingeri.展开更多
Peanut (Arachis hypogaea L.) is one of the most susceptible host crops to Aspergillus flavus invasion and subsequent aflatoxin contamination. In this report, a new member of PR10 family putative resistant gene (design...Peanut (Arachis hypogaea L.) is one of the most susceptible host crops to Aspergillus flavus invasion and subsequent aflatoxin contamination. In this report, a new member of PR10 family putative resistant gene (designated as ARAhPR10, No. EU661964.1) encoding a PR10 protein was isolated and characterized. Analysis of qRT-PCR showed that the expression of ARAhPR10 was induced by pre-harvested A. flavus infection, but no significant difference was observed between resistant genotype “GT-C20” and susceptible genotype “Yueyou 7”. Seven transgenic peanut lines expressing the ARAhPR10 gene under the control of 35S promoter were obtained using the Agrobacterium tumefaciens-mediated method. Real time RT-PCR results showed that the expression level of the ARAhPR10 was significantly higher and the A. flavus infection and aflatoxin content were significantly lower in seeds of transgenic lines than that of the wild type. A significant negative correlation between ARAhPR10 expression at transcript level and seeds aflatoxin production was observed. Combining the previous results, it is suggested that ARAhPR10 expression play an important role in peanut host resistance to A. flavus infection and aflatoxin producing.展开更多
A more natural way for non-expert users to express their tasks in an open-ended set is to use natural language. In this case,a human-centered intelligent agent/robot is required to be able to understand and generate p...A more natural way for non-expert users to express their tasks in an open-ended set is to use natural language. In this case,a human-centered intelligent agent/robot is required to be able to understand and generate plans for these naturally expressed tasks. For this purpose, it is a good way to enhance intelligent robot's abilities by utilizing open knowledge extracted from the web, instead of hand-coded knowledge. A key challenge of utilizing open knowledge lies in the semantic interpretation of the open knowledge organized in multiple modes, which can be unstructured or semi-structured, before one can use it.Previous approaches used a limited lexicon to employ combinatory categorial grammar(CCG) as the underlying formalism for semantic parsing over sentences. Here, we propose a more effective learning method to interpret semi-structured user instructions. Moreover, we present a new heuristic method to recover missing semantic information from the context of an instruction. Experiments showed that the proposed approach renders significant performance improvement compared to the baseline methods and the recovering method is promising.展开更多
Objective: The aim of the study was to investigate inhibitory effects of breast-cancer metastasis suppressor 1 protein (BRMS1) on primary tumor growth and metastasis of human gastric cancer (GC) cells in nude mice. Me...Objective: The aim of the study was to investigate inhibitory effects of breast-cancer metastasis suppressor 1 protein (BRMS1) on primary tumor growth and metastasis of human gastric cancer (GC) cells in nude mice. Methods: We compared the expression of BRMS1 in the primary gastric tumor and metastatic gastric tumor by immunohistochemistry. Expression of BRMS1 also was detected in the GC cells by RT-PCR and Western blot. Three groups of cultured human GC cell line SGC-7901, were maintained: transfected cells with pcDNA3.1(-)B/myc-BRMS1; negative control cells with pcDNA3.1/myc-his(-)B; and blank control cells without any transfection. Histologically intact samples of the cells, maintained by passage in the subcutis of nude mice, were transplanted orthotopically into stomach walls of nude mice to establish a nude mouse model of human gastric carcinoma. Their primary tumor growth and metastasis were then observed. Results: The expression of BRMS1 was markedly stronger in the primary gastric tumor compared with metastatic gastric tumor. We also detected BRMS1 gene and protein in the gastric cancer cell lines. Numbers of metastasis tumors significantly differed among mice infected with transfected cells, with negative controls and with blank controls (4.38 ± 0.60, 7.75 ± 0.59, and 7.63 ± 0.65, respectively; P < 0.05). However, there were no significant differences in the size of orthotopic tumors among mice infected with transfected, negative control and blank control cells [(12.02 ± 0.70), (12.71 ± 0.63) and (12.89 ± 0.71) mm, respectively; P > 0.05]. Conclusion: BRMS1 suppresses metastasis of GC cells, but does not inhibit growth of gastric tumors.展开更多
Objective: To investigate the expression of fragile histidine triad (FHIT) protein in normal colorectal tissue, colorectal adenoma and colorectal cancer (CRC), and to study the relationships between the expression of ...Objective: To investigate the expression of fragile histidine triad (FHIT) protein in normal colorectal tissue, colorectal adenoma and colorectal cancer (CRC), and to study the relationships between the expression of FHIT protein and the clinical pathology, the apoptosis-associated protein (Bcl-2, Bax, Survivin), apoptosis in colorectal cancer. Methods: Tissue microarray (TMA) and immunohistochemistry SP were used to detect the expression of FHIT gene, Bcl-2, Bax and Survivin in 16 cases of the normal colorectal tissue, 16 cases of colorectal adenoma and 80 cases of the colorectal cancer. TUNEL was used to detect the apoptosis index (AI) in 80 cases of the colorectal cancer. Results: (1) The positive rates of FHIT gene expression in normal colorectal tissue, colorectal adenoma and adenocancer were 93.75%, 68.75% and 46.25% respectively. There were no significant differences in the relationships between the FHIT gene expression and histological types, the gender as well as the age (P>0.05). There were significant relationships between FHIT gene expression and lymph node metastasis, histological grades, Duke’s system as well as the 5-year survival rate after operation. (2) The positive rates of Bax, Bcl-2 and Survivin in colorectal adenocancer were 72.50%, 51.25%, 77.50% respectively. The expression of FHIT gene was positively correlated with that of Bcl-2, Bax and Survivin. (3) The mean AI in FHIT negative tumors was significantly lower than that in FHIT positive tumors (P<0.01). Conclusion: FHIT gene may play a role in the oncogenesis and progression of colorectal cancer. The abnormal regulation of apoptosis may play an important role in the pathogenesis of colorectal cancer.展开更多
Objective: To detect the expression and location of TGF-β1, ADAM12 and HB-EGF in primary hepatic carcinoma and study their effect on the growth and metastasis of hepatoma carcinoma cell. Methods: TGF-β1, ADAM12 and ...Objective: To detect the expression and location of TGF-β1, ADAM12 and HB-EGF in primary hepatic carcinoma and study their effect on the growth and metastasis of hepatoma carcinoma cell. Methods: TGF-β1, ADAM12 and HB-EGF were detected by RT-PCR and immunohistochemistry in 30 cases of hepatic carcinoma tissues, 30 cases of adjacent carci- noma tissues and 5 cases of normal hepatic tissues. Results: RT-PCR analyses showed that the mRNA expression of TGF-β1, ADAM12 and HB-EGF were markedly increased in each hepatic carcinoma tissue compared with its adjacent tissue (P < 0.01), but no signal was detected in normal hepatic tissue. Immunohistochemistry showed the same outcome on the expression of above three factors in hepatic tissues as RT-PCR. Proteins location analyses showed the proteins of TGF-β1, ADAM12 and HB-EGF all distributed in the stroma of hepatic carcinoma tissues. The positive correlation was found between TGF-β1 and ADAM12 (r = 0.6137, P < 0.05), as well as ADAM12 and HB-EGF (r = 0.5763, P < 0.05). The protein expression of TGF-β1, ADAM12 and HB-EGF were correlated with the size of tumors, degree of differentiation of hepatoma carcinoma cells, portal vein thrombus and the metastasis of absorbent glands, especially with hepatic cirrhosis caused by hepatitis B virus. Conclu- sion: TGF-β1, ADAM12 and HB-EGF possibly play an important role in the process of growth, invasion and metastasis of hepatoma carcinoma cell, meanwhile, the above three factors may collectively participate in the transition from hepatic cirrhosis caused by hepatitis B virus to hepatocellular carcinoma.展开更多
Liver cancer is the fifth most common malignancy and the second leading cause of cancer-related mortality worldwide.Surgical resection is widely accepted as the only potentially curative therapy for hepatocellular car...Liver cancer is the fifth most common malignancy and the second leading cause of cancer-related mortality worldwide.Surgical resection is widely accepted as the only potentially curative therapy for hepatocellular carcinoma(HCC),the most predominant type of primary liver cancer worldwide.Despite this,the prognosis and 5-year disease-free survival rate of HCC remain poor.展开更多
Resveratrol synthase (RS) is a key enzyme that plays a critical role in the resveratrol synthesis pathway. In this study, six RS genes were isolated and characterized from peanut variety “Zhenzhu Hong” by silico clo...Resveratrol synthase (RS) is a key enzyme that plays a critical role in the resveratrol synthesis pathway. In this study, six RS genes were isolated and characterized from peanut variety “Zhenzhu Hong” by silico cloning and RT-PCR. Bioinformatics analysis showed that deduced amino acid sequences of the six cloned RS genes were highly conserved with a similarity from 95% to 99% when compared to the RS genes which had been deposited at the GenBank. The results of amino acid sequences analysis showed six RS proteins contained the Chal_Sti_Synt_N and ACP_Syn_III_C domains and can be classified to same family but with different evolutionary distance. Expression pattern analysis by QRT-PCR provided evidence indicating that the mRNA of six RS genes were primarily expressed in the peanut shell at different developmental stages with different expression levels, but only lower levels of them were evident in the peanut kernel. The subcellular localization of RS protein in onion epidermal cell was performed by Agrobacterium tumefaciens-mediated transformation and the green fluorescent was monitored by confocal fluorescence microscopy. The results indicated that, RS1 and RS5 were located in the nucleus and plasma membrane respectively, while the RS2, RS3, RS4 and RS6 were located in both nucleus inner membrane and plasma membrane. The data will provide basic information for elucidating the regulatory mechanisms and enzyme kinetics underlying the RS genes in the resveratrol synthase pathway.展开更多
The Buck converter with LC input filter operating in discontinuous output current mode has a high power factor with a constant duty cycle. A Buck converter in this operation mode can reduce the reverse recovery loss o...The Buck converter with LC input filter operating in discontinuous output current mode has a high power factor with a constant duty cycle. A Buck converter in this operation mode can reduce the reverse recovery loss of the freewheeling diode thus increase the efficiency. The operation, power factor analysis and modeling of the converter are studied in this paper. Experimental results are presented to verify the theoretical predictions.展开更多
By adding a suitable LC filter to the input of a Buck converter, a high-power-factor buck converter is proposed. The converter can operate in the discontinuous-output-current mode operation. A Buck converter in this o...By adding a suitable LC filter to the input of a Buck converter, a high-power-factor buck converter is proposed. The converter can operate in the discontinuous-output-current mode operation. A Buck converter in this operation mode features simple control as the constant duty cycle PWM used. The operation condition of the converter is studied. The validity of analysis is verified by Simulation and Experimental results.展开更多
基金中国博士后科学基金(2021M693125)大连市高层次人才创新支持计划(2019RT09)+1 种基金中国科学院洁净能源创新研究院合作基金(DNL202016,DNL202019)中国科学院洁净能源创新研究院-榆林学院联合基金(YLU-DNL Fund 2021002,YLU-DNL Fund 2021009).
基金financially supported by the National Natural Science Foundation of China(21936005,52070114 and 21876093)Tsinghua-Foshan Innovation Special Fund(TFISF).
文摘Thallium(Tl)compounds,highly toxic to biology,are usually released into flue gas during fossil/minerals combustion,and further distributed in water and soil.In this work,we fundamentally investigated the capture of gaseous Tl_(2)O by industrial V2O5-WO3/TiO_(2)catalyst under working condition in Tl-containing flue gas.Experimental and theoretical results indicated that the Tl_(2)O has significant electron-feeding capacity and easily donate electron to unoccupied orbitals of TiO_(2),leading to dismutation of Ti 2p and inartificial formation of p-n junction on TiO_(2)surface,which prompted Tl_(2)O selectively interacted with TiO_(2)in flue gas.Herein,we proposed and verified an effective way to capture gaseous Tl_(2)O,which offered almost the best choice to eliminate Tl emission from flue gas and expanded the function of the TiO_(2)-based catalyst.The formation of p-n junction on commercial V2O5-WO3/TiO_(2)catalyst under working condition was revealed for the first time,which can be a valuable reference for both heterocatalysis and electro/photocatalysis.
基金funded by the National Natural Science Foundation of China(Grand No.U19A2025)Scientific Research Foundation of China Jiliang University.
文摘Improvement of photosynthetic efficiency is a major approach to increase crop yield potential.Previously,we cloned a gene encoding the chloroplast-located putative monooxygenase TCD5,which is essential in plastid development under low temperature in rice(Oryza sativa L.).In this study,the effects of TCD5 on the photosynthesis and the yields were investigated in rice.Two sets of genetic materials with three levels of TCD5 expression,including tcd5 mutant or TCD5 RNAi transgenic lines and TCD5 over-expression transgenic lines in Jiahua1 and Nipponbare backgrounds,were used in the field trails of multi-locations and multi-years.TCD5 positively affected the panicle number and the yield at dosage.Compared with the wild-types,the panicle numbers were 12.4%-14.6%less in tcd5 mutant and 8.3%-38.6%less in TCD5 RNAi lines,but 26.2%-61.8%more in TCD5 over-expression lines.The grain yields per plant were 9.1%-18.4%less in tcd5 mutant and 14.3%-56.7%less in TCD5 RNAi lines,but 6.9%-56.5%more in TCD5 over-expression lines.The measurements of net photosynthetic rate indicated that mutation or knock down of TCD5 decreased the net photosynthetic rate by 10.4%and 15.6%,respectively,while increasing it by 8.9%and 8.7%in the TCD5 over-expression lines in Jiahua1 and Nipponbare backgrounds,respectively.Accordingly,the measurements of chlorophyll fluorescence parameters showed that the electron transport rate and quantum yield decreased in tcd5 mutant or TCD5 RNAi lines but increased in TCD5 overexpression lines,both in Jiahua1 and Nipponbare backgrounds.IP-MS screening revealed that TCD5 interacts with 29 chloroplast proteins involved in chlorophyll synthesis,photo-reactions of the photosynthesis,carbon assimilation and metabolism,energy metabolism,redox balance,protein synthesis and transportation.Two TCD5 interacted proteins,D1 and FBA were effective targets for improving photosynthesis.These results suggest a potentially new strategy for increasing rice yield by enhancing photosynthesis.
基金National Key Research and Development Program(2016YFC0106500800)NationalMajor Scientific Instruments and Equipments Development Project of National Natural Science Foundation of China(81627805)+3 种基金National Natural Science Foundation of China-Guangdong Joint Fund Key Program(U1401254)National Natural Science Foundation of China Mathematics Tianyuan Foundation(12026602)Guangdong Provincial Natural Science Foundation Team Project(6200171)Guangdong Provincial Health Appropriate Technology Promotion Project(20230319214525105,20230322152307666).
文摘Augmented-and mixed-reality technologies have pioneered the realization of real-time fusion and interactive projection for laparoscopic surgeries.Indocyanine green fluorescence imaging technology has enabled anatomical,functional,and radical hepatectomy through tumor identification and localization of target hepatic segments,driving a transformative shift in themanagement of hepatic surgical diseases,moving away from traditional,empirical diagnostic and treatment approaches toward digital,intelligent ones.The Hepatic Surgery Group of the Surgery Branch of the Chinese Medical Association,Digital Medicine Branch of the Chinese Medical Association,Digital Intelligent Surgery Committee of the Chinese Society of ResearchHospitals,and Liver Cancer Committee of the Chinese Medical Doctor Association organized the relevant experts in China to formulate this consensus.This consensus provides a comprehensive outline of the principles,advantages,processes,and key considerations associated with the application of augmented reality and mixed-reality technology combined with indocyanine green fluorescence imaging technology for hepatic segmental and subsegmental resection.The purpose is to streamline and standardize the application of these technologies.
基金supported by National Natural Science Foundation of China (NSFC) (No. 61804103)National Key R&D Program of China (No. 2017YFA0205002)+8 种基金Natural Science Foundation of the Jiangsu Higher Education Institutions of China (Nos. 18KJA535001 and 14KJB 150020)Natural Science Foundation of Jiangsu Province of China (Nos. BK20170343 and BK20180242)China Postdoctoral Science Foundation (No. 2017M610346)State Key Laboratory of Silicon Materials, Zhejiang University (No. SKL2018-03)Nantong Municipal Science and Technology Program (No. GY12017001)Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University (KSL201803)supported by Collaborative Innovation Center of Suzhou Nano Science & Technology, the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the 111 ProjectJoint International Research Laboratory of Carbon-Based Functional Materials and Devices
文摘Continuous deforming always leads to the performance degradation of a flexible triboelectric nanogenerator due to the Young’s modulus mismatch of different functional layers.In this work,we fabricated a fiber-shaped stretchable and tailorable triboelectric nanogenerator(FST-TENG)based on the geometric construction of a steel wire as electrode and ingenious selection of silicone rubber as triboelectric layer.Owing to the great robustness and continuous conductivity,the FST-TENGs demonstrate high stability,stretchability,and even tailorability.For a single device with ~6 cm in length and ~3 mm in diameter,the open-circuit voltage of ~59.7 V,transferred charge of ~23.7 nC,short-circuit current of ~2.67 μA and average power of ~2.13 μW can be obtained at 2.5 Hz.By knitting several FST-TENGs to be a fabric or a bracelet,it enables to harvest human motion energy and then to drive a wearable electronic device.Finally,it can also be woven on dorsum of glove to monitor the movements of gesture,which can recognize every single finger,different bending angle,and numbers of bent finger by analyzing voltage signals.
基金supported by the National Natural Science Foundation of China, No. 30960506, 81160524the Natural Science Foundation of Ningxia Hui Autonomous Region, No. NZ11212+1 种基金the Key Scientific Research Project of Ningxia Hui Autonomous Region Health Department, No. 2012152the Project of Ningxia Medical University, No. XM2011017
文摘Oxysophoridine, a new alkaloid extracted from Sophora alopecuroides L., has been shown to have a protective effect against ischemic brain damage. In this study, a focal cerebral ischemia/reperfusion injury model was established using middle cerebral artery occlusion in mice. Both 62.5, 125, and 250 mg/kg oxysophoridine, via intraperitoneal injection, and 6 mg/kg nimodipine, via intragastric administration, were administered daily for 7 days before modeling. After 24 hours of reperfusion, mice were tested for neurological deficit, cerebral infarct size was assessed and brain tissue was collected. Results showed that oxysophoridine at 125, 250 mg/kg and 6 mg/kg nimodipine could reduce neurological deficit scores, cerebral infarct size and brain water content in mice. These results provided evidence that oxysophoridine plays a protective role in cerebral ischemia/reperfusion injury. In addition, oxysophoridine at 62.5, 125, and 250 mg/kg and 6 mg/kg nimodipine increased adenosine-triphosphate content, and decreased malondialdehyde and nitric oxide content. These compounds enhanced the activities of glutathione-peroxidase, superoxide dismutase, catalase, and lactate dehydrogenase, and decreased the activity of nitric oxide synthase. Protein and mRNA expression levels of N-methyl-D-aspartate receptor subunit NR1 were markedly inhibited in the presence of 250 mg/kg oxysophoridine and 6 mg/kg nimodipine. Our experimental findings indicated that oxysophoridine has a neuroprotective effect against cerebral ischemia/reperfusion injury in mice, and that the effect may be due to its ability to inhibit oxidative stress and expression of the N-methyl-D-aspartate receptor subunit NR1.
基金funded by Natural Science Foundation of China (NSFC) (Grant No. U1432249)the National Key R&D Program of China (Grant 2017YFA0205002)+5 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)supported by Collaborative Innovation Center of Suzhou Nano Science & Technologythe support from China Postdoctoral Science Foundation (2017M610346)Natural Science Foundation of Jiangsu Province of China (BK20170343)Nantong Municipal Science and Technology Programthe support from Jiangsu University National Science Research Program (16KJB110021)
文摘Progress has been developed in harvesting lowfrequency and irregular blue energy using a triboelectric–electromagnetic hybrid generator in recent years. However,the design of the high-efficiency, mechanically durable hybrid structure is still challenging. In this study, we report a fully packaged triboelectric–electromagnetic hybrid generator(TEHG), in which magnets were utilized as the trigger to drive contact–separation-mode triboelectric nanogenerators(CS-TENGs) and coupled with copper coils to operate rotary freestanding-mode electromagnetic generators(RF-EMGs). The magnet pairs that produce attraction were used to transfer the external mechanical energy to the CS-TENGs, and packaging of the CS-TENGpart was achieved to protect it from the ambient environment. Under a rotatory speed of 100 rpm, the CS-TENGs enabled the TEHG to deliver an output voltage, current,and average power of 315.8 V, 44.6 μA, and ~ 90.7 μW,and the output of the RF-EMGs was 0.59 V, 1.78 m A, and 79.6 μW, respectively. The cylinder-like structure made the TEHG more easily driven by water flow and demonstrated to work as a practical power source to charge commercial capacitors. It can charge a 33μF capacitor from 0 to 2.1 V in 84 s, and the stored energy in the capacitor can drive an electronic thermometer and form a self-powered water-temperature sensing system.
基金supported by the EU-China EcoFuel project(FP7,246772)from the European Commission
文摘Photoelectrochemical(PEC) water splitting using solar energy has attracted great attention for generation of renewable hydrogen with less carbon footprint, while there are enormous challenges that still remain for improving solar energy water splitting efficiency, due to limited light harvesting, energy loss associated to fast recombination of photogenerated charge carriers, as well as electrode degradation. This overview focuses on the recent development about catalyst nanomaterials and nanostructures in different PEC water splitting systems. As photoanode, Au nanoparticle-decorated TiO_2 nanowire electrodes exhibited enhanced photoactivity in both the UV and the visible regions due to surface plasmon resonance of Au and showed the largest photocurrent generation of up to 710 nm. Pt/Cd S/CGSe electrodes were developed as photocathode. With the role of p–n heterojunction, the photoelectrode showed high stability and evolved hydrogen continuously for more than 10 days. Further, in the Z-scheme system(Bi_2S_3/TNA as photoanode and Pt/Si PVC as photocathode at the same time), a self-bias(open-circuit voltage Voc= 0.766 V) was formed between two photoelectrodes, which could facilitate photogenerated charge transfers and enhance the photoelectrochemical performance, and which might provide new hints for PEC water splitting. Meanwhile, the existing problems and prospective solutions have also been reviewed.
基金Supported by the National Natural Science Foundation of China (11072088)Guangdong Provincial Water Resources Science and Technology Project (ysk2009-01)
文摘Stability of an ancient landslide in a reservoir area is analyzed by using centrifugal model tests, soil laboratory tests and numerical analysis. Special attention is paid to variation in water level, simulation of large-scale heterogeneous prototype slope, and strength reduction of sliding zone soils after slope sliding. The results of centrifugal model test show that reservoir impounding can reduce sliding resistance at the slope toe, followed by toe collapsing and front cracking of slope. Rapid drawdown can produce hydrodynamic pressure towards reservoir at the front of slope. Deformation is observed in the middle and upper slope, which reduces the slope stability further and forms the pull-typed landslide trend. Reinforcement of slope toe is effective for preventing the progressive failure. The results of laboratory test show that slope toe sliding will lead to the redistribution of soil density and moisture content, which will reduce the shear strength of soil in sliding zone, and the cohesion of immersed soil is reduced gradually and finally vanishes with time. The numerical results show that the strength reduction method used in finite element method (FEM) is very effective in capturing the progressive failure induced by reservoir water level fluctuations, and the evolution of failure surface derived from numerical simulation is very similar to that observed in centrifugal model test.
基金the 38th China Postdoctoral Science Foundation, No. 2005038055 the Scientific and Technological Research Projects of Liaoning Province, No. 2005225003-14the Natural Science Foundation of Liaoning Province, No. 20052204
文摘BACKGROUND: With developments of tissue engineering and genetic engineering, we aim to culture myoblasts, which are characterized by high purity, high quality and high production, for wide application in neural regeneration researches. OBJECTIVE: To modify traditional dissociation method in order to obtain myoblasts, which are characterized by high purity, high quality and high production, and explore the biological properties under in vitro culture. DESIGN: Observational study. SETTING: Basic Institute of Academy of Military Medical Sciences of Chinese PLA. MATERIALS: Four neonatal Wistar rats of 5 days old, both genders and mean body mass of 10 g were selected in this study. The main reagents and devices were detailed as follows: DMEM medium (Gibco Company), fetus bovine serum (FBS, Hycolne Company), collagenase Ⅱ(Sigma Company), trypsin (Sigma Company), dispase Ⅱ (Sigma Company), desmin antibody (Fuzhou Maixin Company), antibody Ⅱ and ABC kit (Wuhan Boster Biotechnology Company), desk centrifuge (KUBATO, Japan), and inverted phase contrast microscope (LEICA DMIRB, Germany). METHODS: The experiment was carried out in the Basic Institute of Academy of Military Medical Sciences of Chinese PLA from June to October 2006. Neonatal rats were sacrificed under sterile condition to obtain skeletal muscles of limbs, which were washed with cold PBS (containing benzylpenicillin and estreptomicina), and muscular tissue was sheared into pieces. Then, those muscular pieces were added with mixed digestive enzyme (containing 2 g/L collagenase Ⅱ + 5 g/L dispase Ⅱ + 0.28 g/L CaCl2) as twice volume as pieces, dealt with mechanical pipetting for 5 minutes and cultured in CO2 incubator for 10 minutes. The operation was done for three times and the muscular pieces were digested for 45 minutes in total. Moreover, cells were suspended again in order to obtain myoblasts from skeletal muscle of neonatal rats. In addition, myoblasts were purified with differential attachment technique and enzyme digestion so as to observe morphological characteristics and growth, draw growth curve, analyze surface structure under scanning electron microscope, and evaluate with Desmin immunohistochemical staining. MAIN OUTCOME MEASURES: Morphological characteristics and growth of myoblasts cultured in vitro. RESULTS: ① Growth of myoblasts of skeletal muscle: Primary cells had well growth, mature and differentiation. The positive rate of Desmin was 94% and purification of cells was ideal. Growth curve of cells demonstrated that myoblasts which were characterized by high purification started proliferation plentiful through transient growth lag phase (about at one or two days after inoculation). If myoblasts were not dealt with any interventions, they might become sarcotubule gradually at 3–5 days after proliferative phase. During this period, myoblasts maintained a monocaryon-bipolarity state under inverted phase contrast microscope. Furthermore, the growth of cells was the strongest and reproductive activity was the most powerful. This suggested that myotube started to form; in addition, muscle fiber of contractility might form under a well culturing condition. ② Immunocytochemical stain with desmin antibody: Interzonal fiber of desmin from myoblasts showed strongly positive reaction. Positive staining existed in cytoplasm had a high nucleus-cytoplasm ratio. However, myoblasts showed negative or mildly positive reaction. CONCLUSION: It is ideal for modified multi-enzymatic digestion and double purification method to dissociate and purify myoblasts of skeletal muscle; meanwhile, these two methods are both the effective ways to provide convenient conditions to obtain seed cells for neural regeneration researches.
基金provided by the National Natural Science Foundation of China (NSFC) to Xiaohong CHEN (Grant Nos.30870277,31372164,31572245)the key discipline of ecology of Henan Province, and the innovation team of science and technology of Henan Province (C20140032)
文摘The objectives of this study are to present a detailed and comprehensive description of the osteology of Quasipaa robertingeri(Anura: Dicroglossidae), to provide osteological evidence for taxonomy, and to explore the adaptive traits of Q. robertingeri. We comprehensively studied the osteology of 2 adult specimens(1 male 98A00351 and 1 female 98A0041) of Q. robertingeri using conventional methods. Our osteological examination of Q. robertingerishows that 1) the nasals are large and connect with one another medially; 2) the sphenethmoid is completely covered by the nasals and the frontopatietals, and thus it is not exposed dorsally; 3) the vomerine teeth are well-developed; 4) the anterior parts of the diapophyses of the presacral II protrude forward in the male, the same observation was made in the presacrals II and III in the female; 5) the pectoral girdle is firmisternal; 6) the omosternum is fan-shaped posteriorly, and almost equal to the length of the mesosternum; 7) the xiphisternum is W-shaped with a deep incision posteriorly; 8) the humerus of the male is more developed in the crista ventralis, the crista medialis, and the crista lateralis; 9) the metacarpal II of the male presents with a nuptial tuber; and 10) the distal phalanges of the digits are anchorshaped. Ultimately, the unique characteristics listed are most likely adaptations to the environment and amplexus of Q. robertingeri.
文摘Peanut (Arachis hypogaea L.) is one of the most susceptible host crops to Aspergillus flavus invasion and subsequent aflatoxin contamination. In this report, a new member of PR10 family putative resistant gene (designated as ARAhPR10, No. EU661964.1) encoding a PR10 protein was isolated and characterized. Analysis of qRT-PCR showed that the expression of ARAhPR10 was induced by pre-harvested A. flavus infection, but no significant difference was observed between resistant genotype “GT-C20” and susceptible genotype “Yueyou 7”. Seven transgenic peanut lines expressing the ARAhPR10 gene under the control of 35S promoter were obtained using the Agrobacterium tumefaciens-mediated method. Real time RT-PCR results showed that the expression level of the ARAhPR10 was significantly higher and the A. flavus infection and aflatoxin content were significantly lower in seeds of transgenic lines than that of the wild type. A significant negative correlation between ARAhPR10 expression at transcript level and seeds aflatoxin production was observed. Combining the previous results, it is suggested that ARAhPR10 expression play an important role in peanut host resistance to A. flavus infection and aflatoxin producing.
基金supported by the National Natural Science Foundation of China(61175057)the USTC Key-Direction Research Fund(WK0110000028)
文摘A more natural way for non-expert users to express their tasks in an open-ended set is to use natural language. In this case,a human-centered intelligent agent/robot is required to be able to understand and generate plans for these naturally expressed tasks. For this purpose, it is a good way to enhance intelligent robot's abilities by utilizing open knowledge extracted from the web, instead of hand-coded knowledge. A key challenge of utilizing open knowledge lies in the semantic interpretation of the open knowledge organized in multiple modes, which can be unstructured or semi-structured, before one can use it.Previous approaches used a limited lexicon to employ combinatory categorial grammar(CCG) as the underlying formalism for semantic parsing over sentences. Here, we propose a more effective learning method to interpret semi-structured user instructions. Moreover, we present a new heuristic method to recover missing semantic information from the context of an instruction. Experiments showed that the proposed approach renders significant performance improvement compared to the baseline methods and the recovering method is promising.
基金Supported by a grant from the Jiangxi Provincial Natural Science Foundation of China (No. 0640063)
文摘Objective: The aim of the study was to investigate inhibitory effects of breast-cancer metastasis suppressor 1 protein (BRMS1) on primary tumor growth and metastasis of human gastric cancer (GC) cells in nude mice. Methods: We compared the expression of BRMS1 in the primary gastric tumor and metastatic gastric tumor by immunohistochemistry. Expression of BRMS1 also was detected in the GC cells by RT-PCR and Western blot. Three groups of cultured human GC cell line SGC-7901, were maintained: transfected cells with pcDNA3.1(-)B/myc-BRMS1; negative control cells with pcDNA3.1/myc-his(-)B; and blank control cells without any transfection. Histologically intact samples of the cells, maintained by passage in the subcutis of nude mice, were transplanted orthotopically into stomach walls of nude mice to establish a nude mouse model of human gastric carcinoma. Their primary tumor growth and metastasis were then observed. Results: The expression of BRMS1 was markedly stronger in the primary gastric tumor compared with metastatic gastric tumor. We also detected BRMS1 gene and protein in the gastric cancer cell lines. Numbers of metastasis tumors significantly differed among mice infected with transfected cells, with negative controls and with blank controls (4.38 ± 0.60, 7.75 ± 0.59, and 7.63 ± 0.65, respectively; P < 0.05). However, there were no significant differences in the size of orthotopic tumors among mice infected with transfected, negative control and blank control cells [(12.02 ± 0.70), (12.71 ± 0.63) and (12.89 ± 0.71) mm, respectively; P > 0.05]. Conclusion: BRMS1 suppresses metastasis of GC cells, but does not inhibit growth of gastric tumors.
基金Supported by the National Science Foundation of Guangdong Province (No. 06020005).
文摘Objective: To investigate the expression of fragile histidine triad (FHIT) protein in normal colorectal tissue, colorectal adenoma and colorectal cancer (CRC), and to study the relationships between the expression of FHIT protein and the clinical pathology, the apoptosis-associated protein (Bcl-2, Bax, Survivin), apoptosis in colorectal cancer. Methods: Tissue microarray (TMA) and immunohistochemistry SP were used to detect the expression of FHIT gene, Bcl-2, Bax and Survivin in 16 cases of the normal colorectal tissue, 16 cases of colorectal adenoma and 80 cases of the colorectal cancer. TUNEL was used to detect the apoptosis index (AI) in 80 cases of the colorectal cancer. Results: (1) The positive rates of FHIT gene expression in normal colorectal tissue, colorectal adenoma and adenocancer were 93.75%, 68.75% and 46.25% respectively. There were no significant differences in the relationships between the FHIT gene expression and histological types, the gender as well as the age (P>0.05). There were significant relationships between FHIT gene expression and lymph node metastasis, histological grades, Duke’s system as well as the 5-year survival rate after operation. (2) The positive rates of Bax, Bcl-2 and Survivin in colorectal adenocancer were 72.50%, 51.25%, 77.50% respectively. The expression of FHIT gene was positively correlated with that of Bcl-2, Bax and Survivin. (3) The mean AI in FHIT negative tumors was significantly lower than that in FHIT positive tumors (P<0.01). Conclusion: FHIT gene may play a role in the oncogenesis and progression of colorectal cancer. The abnormal regulation of apoptosis may play an important role in the pathogenesis of colorectal cancer.
文摘Objective: To detect the expression and location of TGF-β1, ADAM12 and HB-EGF in primary hepatic carcinoma and study their effect on the growth and metastasis of hepatoma carcinoma cell. Methods: TGF-β1, ADAM12 and HB-EGF were detected by RT-PCR and immunohistochemistry in 30 cases of hepatic carcinoma tissues, 30 cases of adjacent carci- noma tissues and 5 cases of normal hepatic tissues. Results: RT-PCR analyses showed that the mRNA expression of TGF-β1, ADAM12 and HB-EGF were markedly increased in each hepatic carcinoma tissue compared with its adjacent tissue (P < 0.01), but no signal was detected in normal hepatic tissue. Immunohistochemistry showed the same outcome on the expression of above three factors in hepatic tissues as RT-PCR. Proteins location analyses showed the proteins of TGF-β1, ADAM12 and HB-EGF all distributed in the stroma of hepatic carcinoma tissues. The positive correlation was found between TGF-β1 and ADAM12 (r = 0.6137, P < 0.05), as well as ADAM12 and HB-EGF (r = 0.5763, P < 0.05). The protein expression of TGF-β1, ADAM12 and HB-EGF were correlated with the size of tumors, degree of differentiation of hepatoma carcinoma cells, portal vein thrombus and the metastasis of absorbent glands, especially with hepatic cirrhosis caused by hepatitis B virus. Conclu- sion: TGF-β1, ADAM12 and HB-EGF possibly play an important role in the process of growth, invasion and metastasis of hepatoma carcinoma cell, meanwhile, the above three factors may collectively participate in the transition from hepatic cirrhosis caused by hepatitis B virus to hepatocellular carcinoma.
文摘Liver cancer is the fifth most common malignancy and the second leading cause of cancer-related mortality worldwide.Surgical resection is widely accepted as the only potentially curative therapy for hepatocellular carcinoma(HCC),the most predominant type of primary liver cancer worldwide.Despite this,the prognosis and 5-year disease-free survival rate of HCC remain poor.
文摘Resveratrol synthase (RS) is a key enzyme that plays a critical role in the resveratrol synthesis pathway. In this study, six RS genes were isolated and characterized from peanut variety “Zhenzhu Hong” by silico cloning and RT-PCR. Bioinformatics analysis showed that deduced amino acid sequences of the six cloned RS genes were highly conserved with a similarity from 95% to 99% when compared to the RS genes which had been deposited at the GenBank. The results of amino acid sequences analysis showed six RS proteins contained the Chal_Sti_Synt_N and ACP_Syn_III_C domains and can be classified to same family but with different evolutionary distance. Expression pattern analysis by QRT-PCR provided evidence indicating that the mRNA of six RS genes were primarily expressed in the peanut shell at different developmental stages with different expression levels, but only lower levels of them were evident in the peanut kernel. The subcellular localization of RS protein in onion epidermal cell was performed by Agrobacterium tumefaciens-mediated transformation and the green fluorescent was monitored by confocal fluorescence microscopy. The results indicated that, RS1 and RS5 were located in the nucleus and plasma membrane respectively, while the RS2, RS3, RS4 and RS6 were located in both nucleus inner membrane and plasma membrane. The data will provide basic information for elucidating the regulatory mechanisms and enzyme kinetics underlying the RS genes in the resveratrol synthase pathway.
文摘The Buck converter with LC input filter operating in discontinuous output current mode has a high power factor with a constant duty cycle. A Buck converter in this operation mode can reduce the reverse recovery loss of the freewheeling diode thus increase the efficiency. The operation, power factor analysis and modeling of the converter are studied in this paper. Experimental results are presented to verify the theoretical predictions.
文摘By adding a suitable LC filter to the input of a Buck converter, a high-power-factor buck converter is proposed. The converter can operate in the discontinuous-output-current mode operation. A Buck converter in this operation mode features simple control as the constant duty cycle PWM used. The operation condition of the converter is studied. The validity of analysis is verified by Simulation and Experimental results.
