Since the 17th National Congress of the Communist Party of China, ecological civilization has been the key construction goal in China, which has a profound impact on the process of urbanization. In recent years, with ...Since the 17th National Congress of the Communist Party of China, ecological civilization has been the key construction goal in China, which has a profound impact on the process of urbanization. In recent years, with the rapid development of urbanization and unreasonable land development and utilization, the ecological environment has deteriorated sharply, and the stability of ecological environment has always been a difficult problem in the process of regional development. The spatial division of urban ecological function can be divided into different types of spatial utilization according to the fragile state of ecological environment, which not only guarantees urban construction but also maintains the ecological environment health, and has great economic benefits and ecological value in formulating regional development strategies and ecological protection. In this paper, from four perspectives of “land, water, human and forest”, eight factors affecting ecological sensitivity were selected, and GIS spatial superposition method was used to conduct qualitative and quantitative analysis on the ecological sensitivity of Baoji City, and the evaluation results of single factor ecological sensitivity and comprehensive ecological sensitivity were obtained. The areas with different ecological sensitivity are divided into extremely sensitive areas, highly sensitive areas, moderately sensitive areas, slightly sensitive areas and non-sensitive areas. The research shows that the proportion of extreme, high, moderate, mild and insensitive areas in Baoji City is 7.32%, 10.57%, 22.25%, 42.91% and 16.95% respectively. Finally, the areas with different sensitivity grades are identified as three types of urban ecological function areas: ecological conservation area, ecological coordinated moderate utilization area and ecological suitable construction area, so as to provide scientific theoretical basis for urban construction and land use optimization in Baoji City.展开更多
Postmenopausal osteoporosis is a common bone metabolic disorder characterized by deterioration of the bone microarchitecture,leading to an increased risk of fractures.Recently,circular RNAs(circ RNAs)have been demonst...Postmenopausal osteoporosis is a common bone metabolic disorder characterized by deterioration of the bone microarchitecture,leading to an increased risk of fractures.Recently,circular RNAs(circ RNAs)have been demonstrated to play pivotal roles in regulating bone metabolism.However,the underlying functions of circ RNAs in bone metabolism in postmenopausal osteoporosis remain obscure.Here,we report that circ Stag1 is a critical osteoporosis-related circ RNA that shows significantly downregulated expression in osteoporotic bone marrow mesenchymal stem cells(BMSCs)and clinical bone tissue samples from patients with osteoporosis.Overexpression of circ Stag1 significantly promoted the osteogenic capability of BMSCs.Mechanistically,we found that circ Stag1 interacts with human antigen R(Hu R),an RNA-binding protein,and promotes the translocation of Hu R into the cytoplasm.A high cytoplasmic level of Hu R led to the activation of the Wnt signaling pathway by stabilizing and enhancing low-density lipoprotein receptor-related protein 5/6(Lrp5/6)andβ-catenin expression,thereby stimulating the osteogenic differentiation of BMSCs.Furthermore,overexpression of circ Stag1 in vivo by circ Stag1-loaded adeno-associated virus(circ Stag1-AAV)promoted new bone formation,thereby preventing bone loss in ovariectomized rats.Collectively,we show that circ Stag1 plays a pivotal role in promoting the regeneration of bone tissue via Hu R/Wnt signaling,which may provide new strategies to prevent bone metabolic disorders such as postmenopausal osteoporosis.展开更多
This study explored the medium for new variety of Asparagus——Linlu No.1 to induce germination, bud proliferation,and induce rootings and their formula. The result revealed that MS+6-BA 1.0 mg/L+NAA 0.05 mg/L is the ...This study explored the medium for new variety of Asparagus——Linlu No.1 to induce germination, bud proliferation,and induce rootings and their formula. The result revealed that MS+6-BA 1.0 mg/L+NAA 0.05 mg/L is the suitable medium for germination; MS+6-BA 0.5 mg/L+NAA 0.5 mg/L is the medium fit for bud proliferation and 1/2MS+IBA 0.5 mg/L+KT 0.05 mg/L+sucrose 30 g/L is the perfect medium for rootings.展开更多
Background and Aims:The aim was to establish a liver venous deprivation(LVD)model in rats,compare hepatic hypertrophy between LVD and associated liver partition and portal vein ligation for staged hepatectomy(ALPPS),a...Background and Aims:The aim was to establish a liver venous deprivation(LVD)model in rats,compare hepatic hypertrophy between LVD and associated liver partition and portal vein ligation for staged hepatectomy(ALPPS),and explore the underlying mechanisms.Methods:The LVD or extended-LVD(e-LVD)group received portal vein ligation(PVL)combined with hepatic vein ligation(HVL).The ALPPS or eALPPS group received PVL plus parenchyma ligation.Liver regeneration was assessed by measuring the liver weight and performing pathological analysis.Liver functions and the sphingosine kinase 1(SPHK1)/sphingosine-1-phosphate(S1P)/sphingosine-1-phosphate receptor 1(S1PR1)pathway were also investigated.Results:All future liver remnants(FLRs)in the ALPPS,e-ALPPS,LVD,and e-LVD groups exhibited significant hypertrophy compared with the control group.The LVD and e-LVD procedures induced similar liver hypertrophy than that in the corresponding ALPPS groups.Furthermore,the LVD and e-LVD methods led to obvious cytolysis in the venous-deprived lobes as well as a noticeable increase in serum transaminase levels,while no necrosis was observed in the ALPPS and e-ALPPS groups.SPHK1/S1P/S1PR1 pathway were distinctly activated after operation,especially in congestive/ischemic livers.Conclusions:We describe the first rat model of LVD and e-LVD with simultaneously associated HVL and PVL.Compared with the ALPPS technique,the LVD or e-LVD procedure had a comparable overall effect on the hypertrophy response and a stronger effect on liver function.The SPHK1/S1P/S1PR1 pathway was involved in the LVD-or ALPPS-induced liver remodeling.展开更多
文摘Since the 17th National Congress of the Communist Party of China, ecological civilization has been the key construction goal in China, which has a profound impact on the process of urbanization. In recent years, with the rapid development of urbanization and unreasonable land development and utilization, the ecological environment has deteriorated sharply, and the stability of ecological environment has always been a difficult problem in the process of regional development. The spatial division of urban ecological function can be divided into different types of spatial utilization according to the fragile state of ecological environment, which not only guarantees urban construction but also maintains the ecological environment health, and has great economic benefits and ecological value in formulating regional development strategies and ecological protection. In this paper, from four perspectives of “land, water, human and forest”, eight factors affecting ecological sensitivity were selected, and GIS spatial superposition method was used to conduct qualitative and quantitative analysis on the ecological sensitivity of Baoji City, and the evaluation results of single factor ecological sensitivity and comprehensive ecological sensitivity were obtained. The areas with different ecological sensitivity are divided into extremely sensitive areas, highly sensitive areas, moderately sensitive areas, slightly sensitive areas and non-sensitive areas. The research shows that the proportion of extreme, high, moderate, mild and insensitive areas in Baoji City is 7.32%, 10.57%, 22.25%, 42.91% and 16.95% respectively. Finally, the areas with different sensitivity grades are identified as three types of urban ecological function areas: ecological conservation area, ecological coordinated moderate utilization area and ecological suitable construction area, so as to provide scientific theoretical basis for urban construction and land use optimization in Baoji City.
基金The Shenzhen Municipal Science and Technology Innovation Committee Project(JCYJ20190806160407178,JCYJ20180305164544288,JSGG20180504170427135,SGLH20180625141602256,JCYJ20180305164659637)the Shenzhen Key Laboratory of Musculoskeletal Tissue Reconstruction and Function Restoration(ZDSYS20200811143752005)supported this work。
文摘Postmenopausal osteoporosis is a common bone metabolic disorder characterized by deterioration of the bone microarchitecture,leading to an increased risk of fractures.Recently,circular RNAs(circ RNAs)have been demonstrated to play pivotal roles in regulating bone metabolism.However,the underlying functions of circ RNAs in bone metabolism in postmenopausal osteoporosis remain obscure.Here,we report that circ Stag1 is a critical osteoporosis-related circ RNA that shows significantly downregulated expression in osteoporotic bone marrow mesenchymal stem cells(BMSCs)and clinical bone tissue samples from patients with osteoporosis.Overexpression of circ Stag1 significantly promoted the osteogenic capability of BMSCs.Mechanistically,we found that circ Stag1 interacts with human antigen R(Hu R),an RNA-binding protein,and promotes the translocation of Hu R into the cytoplasm.A high cytoplasmic level of Hu R led to the activation of the Wnt signaling pathway by stabilizing and enhancing low-density lipoprotein receptor-related protein 5/6(Lrp5/6)andβ-catenin expression,thereby stimulating the osteogenic differentiation of BMSCs.Furthermore,overexpression of circ Stag1 in vivo by circ Stag1-loaded adeno-associated virus(circ Stag1-AAV)promoted new bone formation,thereby preventing bone loss in ovariectomized rats.Collectively,we show that circ Stag1 plays a pivotal role in promoting the regeneration of bone tissue via Hu R/Wnt signaling,which may provide new strategies to prevent bone metabolic disorders such as postmenopausal osteoporosis.
文摘This study explored the medium for new variety of Asparagus——Linlu No.1 to induce germination, bud proliferation,and induce rootings and their formula. The result revealed that MS+6-BA 1.0 mg/L+NAA 0.05 mg/L is the suitable medium for germination; MS+6-BA 0.5 mg/L+NAA 0.5 mg/L is the medium fit for bud proliferation and 1/2MS+IBA 0.5 mg/L+KT 0.05 mg/L+sucrose 30 g/L is the perfect medium for rootings.
基金supported by the Fundamental Research Funds for the Central Universities (NO.2042020kf0124)the National Natural Science Foundation of China (NO.82001940).
文摘Background and Aims:The aim was to establish a liver venous deprivation(LVD)model in rats,compare hepatic hypertrophy between LVD and associated liver partition and portal vein ligation for staged hepatectomy(ALPPS),and explore the underlying mechanisms.Methods:The LVD or extended-LVD(e-LVD)group received portal vein ligation(PVL)combined with hepatic vein ligation(HVL).The ALPPS or eALPPS group received PVL plus parenchyma ligation.Liver regeneration was assessed by measuring the liver weight and performing pathological analysis.Liver functions and the sphingosine kinase 1(SPHK1)/sphingosine-1-phosphate(S1P)/sphingosine-1-phosphate receptor 1(S1PR1)pathway were also investigated.Results:All future liver remnants(FLRs)in the ALPPS,e-ALPPS,LVD,and e-LVD groups exhibited significant hypertrophy compared with the control group.The LVD and e-LVD procedures induced similar liver hypertrophy than that in the corresponding ALPPS groups.Furthermore,the LVD and e-LVD methods led to obvious cytolysis in the venous-deprived lobes as well as a noticeable increase in serum transaminase levels,while no necrosis was observed in the ALPPS and e-ALPPS groups.SPHK1/S1P/S1PR1 pathway were distinctly activated after operation,especially in congestive/ischemic livers.Conclusions:We describe the first rat model of LVD and e-LVD with simultaneously associated HVL and PVL.Compared with the ALPPS technique,the LVD or e-LVD procedure had a comparable overall effect on the hypertrophy response and a stronger effect on liver function.The SPHK1/S1P/S1PR1 pathway was involved in the LVD-or ALPPS-induced liver remodeling.
