Argatroban is a synthetic thrombin inhibitor approved by U.S.Food and Drug Administration for the treatment of thrombosis.However,whether it plays a role in the repair of spinal cord injury is unknown.In this study,we...Argatroban is a synthetic thrombin inhibitor approved by U.S.Food and Drug Administration for the treatment of thrombosis.However,whether it plays a role in the repair of spinal cord injury is unknown.In this study,we established a rat model of T10 moderate spinal cord injury using an NYU Impactor ModerⅢand performed intraperitoneal injection of argatroban for 3 consecutive days.Our results showed that argatroban effectively promoted neurological function recovery after spinal cord injury and decreased thrombin expression and activity in the local injured spinal cord.RNA sequencing transcriptomic analysis revealed that the differentially expressed genes in the argatroban-treated group were enriched in the JAK2/STAT3 pathway,which is involved in astrogliosis and glial scar formation.Western blotting and immunofluorescence results showed that argatroban downregulated the expression of the thrombin receptor PAR1 in the injured spinal cord and the JAK2/STAT3 signal pathway.Argatroban also inhibited the activation and proliferation of astrocytes and reduced glial scar formation in the spinal cord.Taken together,these findings suggest that argatroban may inhibit astrogliosis by inhibiting the thrombin-mediated PAR1/JAK2/STAT3 signal pathway,thereby promoting the recovery of neurological function after spinal cord injury.展开更多
Maintaining the integrity of the blood-spinal cord barrier is critical for the recove ry of spinal cord injury.Ferro ptosis contributes to the pathogenesis of spinal cord injury.We hypothesized that ferroptosis is inv...Maintaining the integrity of the blood-spinal cord barrier is critical for the recove ry of spinal cord injury.Ferro ptosis contributes to the pathogenesis of spinal cord injury.We hypothesized that ferroptosis is involved in disruption of the blood-s pinal cord barrier.In this study,we administe red the ferroptosis inhibitor liproxstatin-1 intraperitoneally after contusive spinal co rd injury in rats.Liproxstatin-1 improved locomotor recovery and somatosensory evoked potential electrophysiological performance after spinal cord inju ry.Liproxstatin-1 maintained blood-spinal cord barrier integrity by upregulation of the expression of tight junction protein.Liproxstatin-1 inhibited ferroptosis of endothelial cell after spinal cord injury,as shown by the immunofluorescence of an endothelial cell marker(rat endothelium cell antigen-1,RECA-1) and fe rroptosis markers Acyl-CoA synthetase long-chain family member 4 and 15-lipoxygenase.Liproxstatin-1reduced brain endothelial cell ferroptosis in vitro by upregulating glutathione peroxidase 4 and downregulating Acyl-CoA synthetase long-chain family member4 and 15-lipoxygenase.Furthermore,inflammatory cell recruitment and astrogliosis were mitigated after liproxstatin-1 treatment.In summary,liproxstatin-1im proved spinal cord injury recovery by inhibiting ferroptosis in endothelial cells and maintaining blood-s pinal co rd barrier integrity.展开更多
Through field plot experiment,we research the influencing factors of cowpea yield and quality after applying boron and molybdenum microelement fertilizer. Results show that the application of boron and molybdenum micr...Through field plot experiment,we research the influencing factors of cowpea yield and quality after applying boron and molybdenum microelement fertilizer. Results show that the application of boron and molybdenum microelement fertilizer can significantly increase the yield and improve the quality of cowpea. Compared with the control,yield per mu increases by 5. 08- 18. 86%; N content increases by 4. 27%-11. 28%; P content increases by 2. 13%- 13. 57%; K content increases by 2. 00%- 18. 48%; crude protein increases by 4. 16%-19. 94%; vitamin C increases by 2. 71%- 11. 79%; nitrate content decreases by 5%- 15%. Besides,it is better to combine boron with molybdenum than to use each of them separately.展开更多
A novel anchorage for long-span suspension bridges,called pile-caisson composite structures,was recently proposed by the authors in an attempt to reduce the construction period and costs.This study aims to investigate...A novel anchorage for long-span suspension bridges,called pile-caisson composite structures,was recently proposed by the authors in an attempt to reduce the construction period and costs.This study aims to investigate the displacement and force behavior of piles in a pile-caisson composite structure under eccentric inclined loading considering different stratum features.To this end,both 1g model tests and three-dimensional numerical simulations were performed.Two groups of 1g model tests were used to validate the finite-element(FE)method.Parametric studies were then performed to investigate the effects of groundwater level,burial depth of the pile-caisson composite structure,and distribution of soil layers on the performance of the pile-caisson composite structure.The numerical analyses indicated that the influence of the groundwater level on the stability of the caisson was much greater than that of the piles.In addition,increasing the burial depth of the pile-caisson composite structure can assist in reducing the displacements and improving the stability of the pile-caisson composite structure.In addition,the distribution of soil layers can significantly affect the stability of the pile-caisson composite structure,especially the soil layer around the caisson.展开更多
We previously identified that hepatitis B virus(HBV)encodes a microRNA(HBV-miR-3)that restrains HBV replication by targeting the HBV transcript.However,whether HBV-miR-3 affects host innate immunity to modulate HBV re...We previously identified that hepatitis B virus(HBV)encodes a microRNA(HBV-miR-3)that restrains HBV replication by targeting the HBV transcript.However,whether HBV-miR-3 affects host innate immunity to modulate HBV replication remains unclear.Here,we examined the vital functions of HBV-miR-3 in the innate immune response after HBV infection.We found that HBV-miR-3 expression gradually increased in a dose-and time-dependent manner in HBV-infected HepG2-NTCP cells.HBV-miR-3 activated the JAK/STAT signaling pathway by downregulating SOCS5 in hepatocytes,thereby enhancing the IFN-induced anti-HBV effect.In addition,HBVmiR-3 in exosomes facilitated the Ml polarization of macrophages.Furthermore,exosomes containing HBV-miR-3 enhanced the secretion of IL-6 via inhibiting the SOCS5-mediated ubiquitination of EGFR.In short,these results demonstrate that HBV-miR-3 activates the innate immune response to restrain HBV replication by multiple pathways,which may suppress HBV-induced acute liver cell injury and affect the progression of persistent HBV infection.展开更多
To describe the distribution of sandstone reservoirs between wells finely,this paper takes the sandstone reservoir of Layer Nm II-4 in Qinhuangdao 32-6 Oilfield for example,and uses the seismic forward simulation and ...To describe the distribution of sandstone reservoirs between wells finely,this paper takes the sandstone reservoir of Layer Nm II-4 in Qinhuangdao 32-6 Oilfield for example,and uses the seismic forward simulation and well loggings to study the channel sandstone reservoir. Under the guidance of the modern rivers and outcrops sandstone distribution pattern,the predicting limitations of the seismic forward simulation about channel sandstone boundaries such as mudstone interlayer between channel sandstone and elevation distance between adjacent channel sandstone are clear. The research shows that the mudstone interlayer can be described by seismic forward simulation seismic when the mudstone interlayer is thicker than 2 m and the channel sandstone is thicker than 10 m because of the appearance of the seismic peak when the main frequency of seismic data is nearly 60 Hz. And the elevation distance between adjacent channel sandstone can be described by the seismic forward simulation when elevation distance is longer than 6 m and the channel sandstone is thicker than 10 m because of the appearance of the seismic peak. And the seismic waveform features of single channel sandstone boundaries such as elevation distance between channels,overbank sandstone and abandoned channels. Under the study mentioned above,the single channel boundaries are described in Layer Nm II-4 of Qinhuangdao 32-6 Oilfield,and the predicting boundaries are confirmed by the chemical tracers. The results show that the channel sandstone reservoir architecture characterization can be improved by the seismic forward simulation.展开更多
Due to a combination of outstanding properties including high melting point,high density,low thermal expansion coefficient,low saturated vapor pressure and excellent thermal conductivity,tungsten(W)parts have been wid...Due to a combination of outstanding properties including high melting point,high density,low thermal expansion coefficient,low saturated vapor pressure and excellent thermal conductivity,tungsten(W)parts have been widely used as electrodes,heating elements,anti-scatter grids for Computed Tomography(CT)equipment,rocket nozzles and divertor materials for nuclear fusion[1].展开更多
基金supported by the Key Project of the National Natural Science Foundation of China,No.81930070(to SF)the National Natural Science Foundation of China,No.81972074(to XY)the Key Program of Natural Science Foundation of Tianjin,No.19JCZDJC34900(to XY)。
文摘Argatroban is a synthetic thrombin inhibitor approved by U.S.Food and Drug Administration for the treatment of thrombosis.However,whether it plays a role in the repair of spinal cord injury is unknown.In this study,we established a rat model of T10 moderate spinal cord injury using an NYU Impactor ModerⅢand performed intraperitoneal injection of argatroban for 3 consecutive days.Our results showed that argatroban effectively promoted neurological function recovery after spinal cord injury and decreased thrombin expression and activity in the local injured spinal cord.RNA sequencing transcriptomic analysis revealed that the differentially expressed genes in the argatroban-treated group were enriched in the JAK2/STAT3 pathway,which is involved in astrogliosis and glial scar formation.Western blotting and immunofluorescence results showed that argatroban downregulated the expression of the thrombin receptor PAR1 in the injured spinal cord and the JAK2/STAT3 signal pathway.Argatroban also inhibited the activation and proliferation of astrocytes and reduced glial scar formation in the spinal cord.Taken together,these findings suggest that argatroban may inhibit astrogliosis by inhibiting the thrombin-mediated PAR1/JAK2/STAT3 signal pathway,thereby promoting the recovery of neurological function after spinal cord injury.
基金National Natural Science Foundation of China,No.81972074 (to XY)Natural Science Foundation of Tianjin,No.19JCZDJC34900 (to XY)National Key Research and Development Project of Stem Cell and Transformation Research,No.2019YFA0112100 (to SF)。
文摘Maintaining the integrity of the blood-spinal cord barrier is critical for the recove ry of spinal cord injury.Ferro ptosis contributes to the pathogenesis of spinal cord injury.We hypothesized that ferroptosis is involved in disruption of the blood-s pinal cord barrier.In this study,we administe red the ferroptosis inhibitor liproxstatin-1 intraperitoneally after contusive spinal co rd injury in rats.Liproxstatin-1 improved locomotor recovery and somatosensory evoked potential electrophysiological performance after spinal cord inju ry.Liproxstatin-1 maintained blood-spinal cord barrier integrity by upregulation of the expression of tight junction protein.Liproxstatin-1 inhibited ferroptosis of endothelial cell after spinal cord injury,as shown by the immunofluorescence of an endothelial cell marker(rat endothelium cell antigen-1,RECA-1) and fe rroptosis markers Acyl-CoA synthetase long-chain family member 4 and 15-lipoxygenase.Liproxstatin-1reduced brain endothelial cell ferroptosis in vitro by upregulating glutathione peroxidase 4 and downregulating Acyl-CoA synthetase long-chain family member4 and 15-lipoxygenase.Furthermore,inflammatory cell recruitment and astrogliosis were mitigated after liproxstatin-1 treatment.In summary,liproxstatin-1im proved spinal cord injury recovery by inhibiting ferroptosis in endothelial cells and maintaining blood-s pinal co rd barrier integrity.
基金Supported by Hainan Natural Science Foundation(311077)
文摘Through field plot experiment,we research the influencing factors of cowpea yield and quality after applying boron and molybdenum microelement fertilizer. Results show that the application of boron and molybdenum microelement fertilizer can significantly increase the yield and improve the quality of cowpea. Compared with the control,yield per mu increases by 5. 08- 18. 86%; N content increases by 4. 27%-11. 28%; P content increases by 2. 13%- 13. 57%; K content increases by 2. 00%- 18. 48%; crude protein increases by 4. 16%-19. 94%; vitamin C increases by 2. 71%- 11. 79%; nitrate content decreases by 5%- 15%. Besides,it is better to combine boron with molybdenum than to use each of them separately.
基金Yunnan Provincial Department of Education （Grant No.： 2012C109）, Ministry of Education, ＂Chun Hui Plan＂ Foundation （Grant No.： Z2012051） and the National Natural Science Foundation of China （Grant No.： 41361020）, Yunnan University Resource Environment and Earth Science research project （Grant No.： 2013CG006） funded.
基金support from the National Natural Science Foundation of China(Grant Nos.51778575,52078457).
文摘A novel anchorage for long-span suspension bridges,called pile-caisson composite structures,was recently proposed by the authors in an attempt to reduce the construction period and costs.This study aims to investigate the displacement and force behavior of piles in a pile-caisson composite structure under eccentric inclined loading considering different stratum features.To this end,both 1g model tests and three-dimensional numerical simulations were performed.Two groups of 1g model tests were used to validate the finite-element(FE)method.Parametric studies were then performed to investigate the effects of groundwater level,burial depth of the pile-caisson composite structure,and distribution of soil layers on the performance of the pile-caisson composite structure.The numerical analyses indicated that the influence of the groundwater level on the stability of the caisson was much greater than that of the piles.In addition,increasing the burial depth of the pile-caisson composite structure can assist in reducing the displacements and improving the stability of the pile-caisson composite structure.In addition,the distribution of soil layers can significantly affect the stability of the pile-caisson composite structure,especially the soil layer around the caisson.
基金This work was supported in part by the National Natural Science Foundation of China(91629302,81830094,81572790,31270818,and 81773002)the Natural Science Foundation of Tianjin(19JCZDJC35900 and 16JCYBJC42400).
文摘We previously identified that hepatitis B virus(HBV)encodes a microRNA(HBV-miR-3)that restrains HBV replication by targeting the HBV transcript.However,whether HBV-miR-3 affects host innate immunity to modulate HBV replication remains unclear.Here,we examined the vital functions of HBV-miR-3 in the innate immune response after HBV infection.We found that HBV-miR-3 expression gradually increased in a dose-and time-dependent manner in HBV-infected HepG2-NTCP cells.HBV-miR-3 activated the JAK/STAT signaling pathway by downregulating SOCS5 in hepatocytes,thereby enhancing the IFN-induced anti-HBV effect.In addition,HBVmiR-3 in exosomes facilitated the Ml polarization of macrophages.Furthermore,exosomes containing HBV-miR-3 enhanced the secretion of IL-6 via inhibiting the SOCS5-mediated ubiquitination of EGFR.In short,these results demonstrate that HBV-miR-3 activates the innate immune response to restrain HBV replication by multiple pathways,which may suppress HBV-induced acute liver cell injury and affect the progression of persistent HBV infection.
基金supported by the China National Petroleum Corporation Major Project (No. 2011E2506)
文摘To describe the distribution of sandstone reservoirs between wells finely,this paper takes the sandstone reservoir of Layer Nm II-4 in Qinhuangdao 32-6 Oilfield for example,and uses the seismic forward simulation and well loggings to study the channel sandstone reservoir. Under the guidance of the modern rivers and outcrops sandstone distribution pattern,the predicting limitations of the seismic forward simulation about channel sandstone boundaries such as mudstone interlayer between channel sandstone and elevation distance between adjacent channel sandstone are clear. The research shows that the mudstone interlayer can be described by seismic forward simulation seismic when the mudstone interlayer is thicker than 2 m and the channel sandstone is thicker than 10 m because of the appearance of the seismic peak when the main frequency of seismic data is nearly 60 Hz. And the elevation distance between adjacent channel sandstone can be described by the seismic forward simulation when elevation distance is longer than 6 m and the channel sandstone is thicker than 10 m because of the appearance of the seismic peak. And the seismic waveform features of single channel sandstone boundaries such as elevation distance between channels,overbank sandstone and abandoned channels. Under the study mentioned above,the single channel boundaries are described in Layer Nm II-4 of Qinhuangdao 32-6 Oilfield,and the predicting boundaries are confirmed by the chemical tracers. The results show that the channel sandstone reservoir architecture characterization can be improved by the seismic forward simulation.
基金support from the National Natural Science Foundation of China(Nos.52022072,51972243,92060202 and 51521001)the Research Fund for Central Universities(Nos.2020IVB074 and 2021IVA094)+4 种基金the Creative Research Foundation of the Science and Technology on Thermostructural Composite Materials Laboratory(No.6142911180202)supported by Key R&D Program of Guangdong Province(No.2020B090923002)the China Postdoctoral Science Foundation(No.2021T140140)Guangzhou Project of Science&Technology(Nos.202102020327,202007020008)Guangdong province Science and Technology Plan Projects(No.2019A1515011841)。
文摘Due to a combination of outstanding properties including high melting point,high density,low thermal expansion coefficient,low saturated vapor pressure and excellent thermal conductivity,tungsten(W)parts have been widely used as electrodes,heating elements,anti-scatter grids for Computed Tomography(CT)equipment,rocket nozzles and divertor materials for nuclear fusion[1].
