It has been observed that the consumption of litchi often causes symptoms characterized by itching or sore throat,gum swelling,oral cavity ulcers and even fever and inflammation,which significantly impair the quality ...It has been observed that the consumption of litchi often causes symptoms characterized by itching or sore throat,gum swelling,oral cavity ulcers and even fever and inflammation,which significantly impair the quality of life of a large population.Using the RAW264.7 cell line,a step-by-step strategy was used to screen for the components in litchi fruits that elicited adverse reactions.The adverse reaction fractions were identified by mass spectrometry and analyzed using the SMART program,and a sequence alignment of the homologous proteins was performed.MTT tests were used to determine the cytotoxicity of a litchi protein extract in RAW264.7 macrophages,and real-time PCR was applied to analyze the expression of inflammatory genes in the RAW264.7 cells treated with lipopolysaccharide or the litchi protein extract.The results showed that the litchi water-soluble protein extract could increase the production of the pro-inflammatory mediators IL-1β,iNOS and COX-2,and the anti-inflammatory mediator HO-1 in the RAW264.7 cell line.The 14-3-3-like proteins GF14 lambda,GF14 omega and GF14 upsilon were likely the candidate proteins that caused the adverse effects.展开更多
This paper considers the asymptotic dynamics of steady states to the Lotka-Volterra competition diffusion systems with random perturbations by two-parameter white noise on the whole real line. By the fundamental solut...This paper considers the asymptotic dynamics of steady states to the Lotka-Volterra competition diffusion systems with random perturbations by two-parameter white noise on the whole real line. By the fundamental solution of heat equation, we get the asymptotic fluctuating behaviors near the stable states respectively. That is, near the steady state (u,v)=(0,1), the mean value Eu(x,t) is shifted above the equilibrium u=0 and Ev(x,t) is shifted below the equilibrium v=1. However, near the steady state (u,v)=(1,0), the mean value Eu(x,t) is shifted below the equilibrium u =1 and Eu(x,t)=0.展开更多
Background Patients with insulo-Sylvian gliomas continue to present with severe morbidity in cognitive functions primarily due to neurosurgeons’lack of familiarity with non-traditional brain networks.We sought to ide...Background Patients with insulo-Sylvian gliomas continue to present with severe morbidity in cognitive functions primarily due to neurosurgeons’lack of familiarity with non-traditional brain networks.We sought to identify the frequency of invasion and proximity of gliomas to portions of these networks.Methods We retrospectively analyzed data from 45 patients undergoing glioma surgery centered in the insular lobe.Tumors were categorized based on their proximity and invasiveness of non-traditional cognitive networks and traditionally eloquent structures.Diffusion tensor imaging tractography was completed by creating a personalized brain atlas using Quicktome to determine eloquent and non-eloquent networks in each patient.Additionally,we prospectively collected neuropsychological data on 7 patients to compare tumor-network involvement with change in cognition.Lastly,2 prospective patients had their surgical plan influenced by network mapping determined by Quicktome.Results Forty-four of 45 patients demonstrated tumor involvement(<1 cm proximity or invasion)with components of non-traditional brain networks involved in cognition such as the salience network(SN,60%)and the central executive network(CEN,56%).Of the seven prospective patients,all had tumors involved with the SN,CEN(5/7,71%),and language network(5/7,71%).The mean scores of MMSE and MOCA before surgery were 18.71±6.94 and 17.29±6.26,respectively.The two cases who received preoperative planning with Quicktome had a postoperative performance that was anticipated.Conclusions Non-traditional brain networks involved in cognition are encountered during surgical resection of insulo-Sylvian gliomas.Quicktome can improve the understanding of the presence of these networks and allow for more informed surgical decisions based on patient functional goals.展开更多
Background:Rheumatoid arthritis(RA)is an autoimmune disease characterized by the overproliferation of synovial fibroblast-like synoviocytes(FLSs)in the lining,leading to chronic inflammation and progressive joint dama...Background:Rheumatoid arthritis(RA)is an autoimmune disease characterized by the overproliferation of synovial fibroblast-like synoviocytes(FLSs)in the lining,leading to chronic inflammation and progressive joint damage.RA pathogenesis involves lymphocyte infiltration,increased synovial cell proliferation,and impaired cell death.This study investigated the effects of rapamycin on RA-FLSs and explored the underlying molecular mechanisms.Methods:The optimal drug concentration and time for rapamycin administration were determined using a cell counting kit-8 assay.The concentration of rapamycin varied from 1 to 25 nmol/L.The mRNA expression levels of protein kinase B(AKT),mammalian target of rapamycin(mTOR),B cell lymphoma 2(Bcl-2),and Bcl-2-associated X protein(Bax)were quantified by real-time polymerase chain reaction.Protein expression(p-AKT,AKT,p-mTOR,mTOR,pS6 kinase[S6K],S6K,p-4E-binding protein 1[4EBP1],4EBP1,Bcl-2,Bax,caspase 3,caspase 9,cyclin-dependent kinase 2[CDK2],and CD1)were detected by Western blotting analysis.Results:Rapamycin suppresses RA-FLS proliferation and induces apoptosis in a dose-dependent manner.Rapamycin significantly elevated the protein expression of Bax(p<0.01),caspase 3(p<0.05),and caspase 9(p<0.001),and downregulated Bcl-2 expression(p<0.01).Rapamycin increased Bax mRNA expression(p<0.01)and decreased Bcl-2(p<0.05),AKT(p<0.05),and mTOR(p<0.05)expression.Additionally,there was a marked reduction in the expression levels of p-AKT(Ser473)(p<0.01),p-mTOR(Ser2448)(p<0.01),p-S6K1(p<0.01),and p-4EBP1,CDK2,and CD1(p<0.01).Conclusions:Rapamycin effectively inhibited proliferation and induced apoptosis of RA-FLSs by targeting the AKT/mTORC1 pathway.These findings underscore the potential of rapamycin as a therapeutic candidate for addressing the dysregulated proliferation and inflammatory characteristics of RA.Further clinical investigations are required to validate its application in the management of RA.展开更多
Synthetic hexaploid wheat （Triticum turgidum x Aegilops tauschii） was created to explore for novel genes from T. turgidum and Ae. tauschii that can be used for common wheat improvement. In the present paper, researc...Synthetic hexaploid wheat （Triticum turgidum x Aegilops tauschii） was created to explore for novel genes from T. turgidum and Ae. tauschii that can be used for common wheat improvement. In the present paper, research advances on the utilization of synthetic hexaploid wheat for wheat genetic improvement in China are reviewed. Over 200 synthetic hexaploid wheat （SHW） accessions from the International Maize and Wheat Improvement Centre （CIMMYT） were introduced into China since 1995. Four cultivars derived from these, Chuanmai 38, Chuanmai 42, Chuanmai 43 and Chuanmai 47, have been released in China. Of these, Chuanmai 42, with large kernels and resistance to stripe rust, had the highest average yield （〉 6 t/ha） among all cultivars over two years in Sichuan provincial yield trials, outyielding the commercial check cultivar Chuanmai 107 by 22,7%. Meanwhile, by either artificial chromosome doubling via colchicine treatment or spontaneous chromosome doubling via a union of unreduced gametes （2n） from T. turgidum-Ae, tauschii hybrids, new SHW lines were produced in China. Mitotic-like meiosis might be the cytological mechanism of spontaneous chromosome doubling. SHW lines with genes for spontaneous chromosome doubling may be useful for producing new SHW-alien amphidiploids and double haploid in wheat genetic improvement.展开更多
基金This work was supported by the Special Fund for Agro-scientific Research in the Public Interest(No.201403036)the Fundamental Research Funds for the Central Universities(2014PY030)+1 种基金the National Basic Research Program of China(2011CB100600)the National NSF of China and the Foundation for the Key Program of the Ministry of Education,China(311029).
文摘It has been observed that the consumption of litchi often causes symptoms characterized by itching or sore throat,gum swelling,oral cavity ulcers and even fever and inflammation,which significantly impair the quality of life of a large population.Using the RAW264.7 cell line,a step-by-step strategy was used to screen for the components in litchi fruits that elicited adverse reactions.The adverse reaction fractions were identified by mass spectrometry and analyzed using the SMART program,and a sequence alignment of the homologous proteins was performed.MTT tests were used to determine the cytotoxicity of a litchi protein extract in RAW264.7 macrophages,and real-time PCR was applied to analyze the expression of inflammatory genes in the RAW264.7 cells treated with lipopolysaccharide or the litchi protein extract.The results showed that the litchi water-soluble protein extract could increase the production of the pro-inflammatory mediators IL-1β,iNOS and COX-2,and the anti-inflammatory mediator HO-1 in the RAW264.7 cell line.The 14-3-3-like proteins GF14 lambda,GF14 omega and GF14 upsilon were likely the candidate proteins that caused the adverse effects.
文摘This paper considers the asymptotic dynamics of steady states to the Lotka-Volterra competition diffusion systems with random perturbations by two-parameter white noise on the whole real line. By the fundamental solution of heat equation, we get the asymptotic fluctuating behaviors near the stable states respectively. That is, near the steady state (u,v)=(0,1), the mean value Eu(x,t) is shifted above the equilibrium u=0 and Ev(x,t) is shifted below the equilibrium v=1. However, near the steady state (u,v)=(1,0), the mean value Eu(x,t) is shifted below the equilibrium u =1 and Eu(x,t)=0.
基金supported by a grant from the National Natural Science Foundation of China(81974389)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘Background Patients with insulo-Sylvian gliomas continue to present with severe morbidity in cognitive functions primarily due to neurosurgeons’lack of familiarity with non-traditional brain networks.We sought to identify the frequency of invasion and proximity of gliomas to portions of these networks.Methods We retrospectively analyzed data from 45 patients undergoing glioma surgery centered in the insular lobe.Tumors were categorized based on their proximity and invasiveness of non-traditional cognitive networks and traditionally eloquent structures.Diffusion tensor imaging tractography was completed by creating a personalized brain atlas using Quicktome to determine eloquent and non-eloquent networks in each patient.Additionally,we prospectively collected neuropsychological data on 7 patients to compare tumor-network involvement with change in cognition.Lastly,2 prospective patients had their surgical plan influenced by network mapping determined by Quicktome.Results Forty-four of 45 patients demonstrated tumor involvement(<1 cm proximity or invasion)with components of non-traditional brain networks involved in cognition such as the salience network(SN,60%)and the central executive network(CEN,56%).Of the seven prospective patients,all had tumors involved with the SN,CEN(5/7,71%),and language network(5/7,71%).The mean scores of MMSE and MOCA before surgery were 18.71±6.94 and 17.29±6.26,respectively.The two cases who received preoperative planning with Quicktome had a postoperative performance that was anticipated.Conclusions Non-traditional brain networks involved in cognition are encountered during surgical resection of insulo-Sylvian gliomas.Quicktome can improve the understanding of the presence of these networks and allow for more informed surgical decisions based on patient functional goals.
基金National Science Foundation of China,Grand/Award Number:82001740Natural Science Foundation of Shanxi Province,Grand/Award Number:202203021221269。
文摘Background:Rheumatoid arthritis(RA)is an autoimmune disease characterized by the overproliferation of synovial fibroblast-like synoviocytes(FLSs)in the lining,leading to chronic inflammation and progressive joint damage.RA pathogenesis involves lymphocyte infiltration,increased synovial cell proliferation,and impaired cell death.This study investigated the effects of rapamycin on RA-FLSs and explored the underlying molecular mechanisms.Methods:The optimal drug concentration and time for rapamycin administration were determined using a cell counting kit-8 assay.The concentration of rapamycin varied from 1 to 25 nmol/L.The mRNA expression levels of protein kinase B(AKT),mammalian target of rapamycin(mTOR),B cell lymphoma 2(Bcl-2),and Bcl-2-associated X protein(Bax)were quantified by real-time polymerase chain reaction.Protein expression(p-AKT,AKT,p-mTOR,mTOR,pS6 kinase[S6K],S6K,p-4E-binding protein 1[4EBP1],4EBP1,Bcl-2,Bax,caspase 3,caspase 9,cyclin-dependent kinase 2[CDK2],and CD1)were detected by Western blotting analysis.Results:Rapamycin suppresses RA-FLS proliferation and induces apoptosis in a dose-dependent manner.Rapamycin significantly elevated the protein expression of Bax(p<0.01),caspase 3(p<0.05),and caspase 9(p<0.001),and downregulated Bcl-2 expression(p<0.01).Rapamycin increased Bax mRNA expression(p<0.01)and decreased Bcl-2(p<0.05),AKT(p<0.05),and mTOR(p<0.05)expression.Additionally,there was a marked reduction in the expression levels of p-AKT(Ser473)(p<0.01),p-mTOR(Ser2448)(p<0.01),p-S6K1(p<0.01),and p-4EBP1,CDK2,and CD1(p<0.01).Conclusions:Rapamycin effectively inhibited proliferation and induced apoptosis of RA-FLSs by targeting the AKT/mTORC1 pathway.These findings underscore the potential of rapamycin as a therapeutic candidate for addressing the dysregulated proliferation and inflammatory characteristics of RA.Further clinical investigations are required to validate its application in the management of RA.
基金supported by the National 863 pro-gram (No. 2006AA10Z1C6)the National Natural Science Foundation of China (No. 30771338 and 30700495)+1 种基金"100-Talent Program" of Chinese Academy of Sciencesthe Science and Technology Department of Sichuan Province
文摘Synthetic hexaploid wheat （Triticum turgidum x Aegilops tauschii） was created to explore for novel genes from T. turgidum and Ae. tauschii that can be used for common wheat improvement. In the present paper, research advances on the utilization of synthetic hexaploid wheat for wheat genetic improvement in China are reviewed. Over 200 synthetic hexaploid wheat （SHW） accessions from the International Maize and Wheat Improvement Centre （CIMMYT） were introduced into China since 1995. Four cultivars derived from these, Chuanmai 38, Chuanmai 42, Chuanmai 43 and Chuanmai 47, have been released in China. Of these, Chuanmai 42, with large kernels and resistance to stripe rust, had the highest average yield （〉 6 t/ha） among all cultivars over two years in Sichuan provincial yield trials, outyielding the commercial check cultivar Chuanmai 107 by 22,7%. Meanwhile, by either artificial chromosome doubling via colchicine treatment or spontaneous chromosome doubling via a union of unreduced gametes （2n） from T. turgidum-Ae, tauschii hybrids, new SHW lines were produced in China. Mitotic-like meiosis might be the cytological mechanism of spontaneous chromosome doubling. SHW lines with genes for spontaneous chromosome doubling may be useful for producing new SHW-alien amphidiploids and double haploid in wheat genetic improvement.
