Anxiety disorder is a common mental disorder. It is necessary to establish a rapid, stable and specific anxiety model to provide a theoretical basis for further research on the pathogenesis of anxiety and drug develop...Anxiety disorder is a common mental disorder. It is necessary to establish a rapid, stable and specific anxiety model to provide a theoretical basis for further research on the pathogenesis of anxiety and drug development. A single intraperitoneal injection of m-chlorophenylpipera-zine (mCPP) (1, 2, 4 mg/kg) was given to male ICR mice to establish an anxiety model, and the effects of mCPP on anxiety behavior, pain, athletic ability, passive avoidance response ability and depressive behavior of male ICR mice were evaluated. A single intraperitoneal injection of mCPP shortened the time in open arms and decreased the percentage of time in open arms of mice in the elevated plus-maze test. mCPP also shortened center zone distance and reduced the number of entries to the central zone in the open field test. Moreover, mCPP reduced head-dip counts and increased the head-dip latency of mice in the hole-board test. After being administrated with a single intraperitoneal injection of mCPP for 24h, the mice showed no significant difference in the entry into the light side and the percentage of time in the light side of the light-dark box test. A single intraperitoneal injection of mCPP had no effects on tail flick latency, rotating time, number of errors and the step-down latency, the immobility time of mice in the tail-flick test, rotarod test, step-down test and TST respectively. In conclusion, we established a rapid and stable anxiety mouse model by single intraperitoneal injection of mCPP.展开更多
Based on single-cell sequencing of the hippocampi of 5×familiar Alzheimer's disease(5×FAD)and wild type mice at 2-,12-,and 24-month of age,we found an increased percentage of microglia in aging and Alzhe...Based on single-cell sequencing of the hippocampi of 5×familiar Alzheimer's disease(5×FAD)and wild type mice at 2-,12-,and 24-month of age,we found an increased percentage of microglia in aging and Alzheimer's disease(AD)mice.Blood brain barrier injury may also have contributed to this increase.Immune regulation by microglia plays a major role in the progression of aging and AD,according to the functions of 41 intersecting differentially expressed genes in microglia.Signaling crosstalk between C−C motif chemokine ligand(CCL)and major histocompatibility complex-1 bridges intercellular communication in the hippocampus during aging and AD.The amyloid precursor protein(APP)and colony stimulating factor(CSF)signals drive 5×FAD to deviate from aging track to AD occurrence among intercellular communication in hippocampus.Microglia are involved in the progression of aging and AD can be divided into 10 functional types.The strength of the interaction among microglial subtypes weakened with aging,and the CCL and CSF signaling pathways were the fundamental bridge of communication among microglial subtypes.展开更多
Traditional Chinese Medicine prescription Suanzaoren decoction (SZRD) is composed of Ziziphi Spinosae Semen, Chuanxiong Rhizoma, Anemarrhenae rhizoma, Poria and Licorice. It was used to treat central nervous system di...Traditional Chinese Medicine prescription Suanzaoren decoction (SZRD) is composed of Ziziphi Spinosae Semen, Chuanxiong Rhizoma, Anemarrhenae rhizoma, Poria and Licorice. It was used to treat central nervous system diseases such as insomnia and anxiety for thousands of years. This paper aims to systematically understand varieties and quantities of compounds and clarify chemical components of SZRD, subsequently to further provide the reference for phytochemistry and pharmacology researches of SZRD. Our results showed that SZRD contained 145 components, including flavonoids, triterpenoids, steroids, coumarins, phthalides, and volatile oils, etc., while five-single herbs contain 1104 components. Only in terms of compound number, there were 80 common components in SZRD and its five herbs, which accounted for 6.8% of total compounds in all 5 herbs and 55.2% of compounds in SZRD. The components of SZRD were not simply the sum of one in every single herb. It is necessary to perform parallel studies among SZRD and its herbs. This review discussed the problems that existed in the chemical research of SZRD and pointed out the direction for its further research.展开更多
In order to study the influence of the wrap angle relating to the space guide vane of a submersible well pump(250QJ125)on the flow field and pump performance,seven possible configurations have been considered(obtained...In order to study the influence of the wrap angle relating to the space guide vane of a submersible well pump(250QJ125)on the flow field and pump performance,seven possible configurations have been considered(obtained by changing the blade wrap angle while keeping unchanged all the other parameters).Such configurations have been numerically simulated in the framework of a computational model based on the Reynolds time-averaged N-S equations,the RNG k-εturbulence approach and the SIMPLE algorithm.The impact exerted by different wrap angles of the guide vane on the performance of the pump,the internal losses of the guide vane and the flow field distribution in the bladeless area at the guide vane outlet has been assessed via cross-comparison of all these cases.The results show that the wrap angle has a significant influence:the wrap angle with the highest head is different from that with the highest efficiency,and changes in this angle have a more significant effect on the head than efficiency.A moderate raise of the wrap angle can improve the properties of the flow,reduce turbulence losses and enhance the energy conversion rate inside the guide vane.Different wrap angles can also lead to different fluid circulation modes in the bladeless area from guide vane outlet to impeller inlet,while they have a weak influence on the absolute value of the velocity of the fluid entering the impeller.展开更多
The influence of the axial mount position of the guide vane on the pressure fluctuation in a nuclear pump(AP1000)is investigated.The characteristics of the three-dimensional flow inside the nuclear pump are analyzed b...The influence of the axial mount position of the guide vane on the pressure fluctuation in a nuclear pump(AP1000)is investigated.The characteristics of the three-dimensional flow inside the nuclear pump are analyzed by means of numerical simulation.Results indicate that when the axial relative distance between the guide vane and the pumping chamber is reduced,in conditions of“small flow,”the efficiency of the pump increases,the pressure inside the pumping chamber decreases,while the losses related to the guide vane grow.Under large flow conditions,as the efficiency of the pump decreases,the losses for the guide vane and the pumping chamber increase.The pressure fluctuation in the annular pumping chamber is basically determined by the rotation frequency and the blade passing frequency.The magnitude of these fluctuations is affected by the guide vane axial position.In particular,the smallest possible amplitude is obtained when the outlet central plane of the guide vane coincides with the outlet axis of the pumping chamber.展开更多
An AP1000 nuclear reactor coolant pump is considered to assess the influence of the Impeller/Guide vane clearance on the performances of this type of pumps.Experiments and numerical simulations relying on an unidirect...An AP1000 nuclear reactor coolant pump is considered to assess the influence of the Impeller/Guide vane clearance on the performances of this type of pumps.Experiments and numerical simulations relying on an unidirectional fluid-solid coupling approach are used to investigate the problem(stress,strain and mode of the rotor).The results reveal the relationship existing between the hydraulic performance of the nuclear reactor coolant pump and the clearance ratio.The effect of clearance ratio on the maximum equivalent stress on the back surface of the impeller blade is greater than that on the working surface(the maximum equivalent stress on the back surface of impeller blade is about three times that on the working surface).The clearance ratio has a scarce effect on the first six natural frequencies of the rotor of the nuclear reactor coolant pump.The related vibrational modes have different waveforms.展开更多
文摘Anxiety disorder is a common mental disorder. It is necessary to establish a rapid, stable and specific anxiety model to provide a theoretical basis for further research on the pathogenesis of anxiety and drug development. A single intraperitoneal injection of m-chlorophenylpipera-zine (mCPP) (1, 2, 4 mg/kg) was given to male ICR mice to establish an anxiety model, and the effects of mCPP on anxiety behavior, pain, athletic ability, passive avoidance response ability and depressive behavior of male ICR mice were evaluated. A single intraperitoneal injection of mCPP shortened the time in open arms and decreased the percentage of time in open arms of mice in the elevated plus-maze test. mCPP also shortened center zone distance and reduced the number of entries to the central zone in the open field test. Moreover, mCPP reduced head-dip counts and increased the head-dip latency of mice in the hole-board test. After being administrated with a single intraperitoneal injection of mCPP for 24h, the mice showed no significant difference in the entry into the light side and the percentage of time in the light side of the light-dark box test. A single intraperitoneal injection of mCPP had no effects on tail flick latency, rotating time, number of errors and the step-down latency, the immobility time of mice in the tail-flick test, rotarod test, step-down test and TST respectively. In conclusion, we established a rapid and stable anxiety mouse model by single intraperitoneal injection of mCPP.
基金supported by grants from the Key R&D Plan of the Science and Technology Plan of Tibet Autonomous Region,China(Grant No.:XZ202201ZY0026G)the Science and Technology Cooperation Project of Shandong Provincial Department of Science and Technology for Counterpart Assistance to Tibet,China(Grant No.:YDZX2021083)+1 种基金the National Natural Science Foundation of China(Grant No.:82205078)the Natural Science Foundation of Shandong Province,China(Grant No.:ZR2021QH157).
文摘Based on single-cell sequencing of the hippocampi of 5×familiar Alzheimer's disease(5×FAD)and wild type mice at 2-,12-,and 24-month of age,we found an increased percentage of microglia in aging and Alzheimer's disease(AD)mice.Blood brain barrier injury may also have contributed to this increase.Immune regulation by microglia plays a major role in the progression of aging and AD,according to the functions of 41 intersecting differentially expressed genes in microglia.Signaling crosstalk between C−C motif chemokine ligand(CCL)and major histocompatibility complex-1 bridges intercellular communication in the hippocampus during aging and AD.The amyloid precursor protein(APP)and colony stimulating factor(CSF)signals drive 5×FAD to deviate from aging track to AD occurrence among intercellular communication in hippocampus.Microglia are involved in the progression of aging and AD can be divided into 10 functional types.The strength of the interaction among microglial subtypes weakened with aging,and the CCL and CSF signaling pathways were the fundamental bridge of communication among microglial subtypes.
文摘Traditional Chinese Medicine prescription Suanzaoren decoction (SZRD) is composed of Ziziphi Spinosae Semen, Chuanxiong Rhizoma, Anemarrhenae rhizoma, Poria and Licorice. It was used to treat central nervous system diseases such as insomnia and anxiety for thousands of years. This paper aims to systematically understand varieties and quantities of compounds and clarify chemical components of SZRD, subsequently to further provide the reference for phytochemistry and pharmacology researches of SZRD. Our results showed that SZRD contained 145 components, including flavonoids, triterpenoids, steroids, coumarins, phthalides, and volatile oils, etc., while five-single herbs contain 1104 components. Only in terms of compound number, there were 80 common components in SZRD and its five herbs, which accounted for 6.8% of total compounds in all 5 herbs and 55.2% of compounds in SZRD. The components of SZRD were not simply the sum of one in every single herb. It is necessary to perform parallel studies among SZRD and its herbs. This review discussed the problems that existed in the chemical research of SZRD and pointed out the direction for its further research.
基金supported by the National Natural Science Foundation of China(No.51469013).
文摘In order to study the influence of the wrap angle relating to the space guide vane of a submersible well pump(250QJ125)on the flow field and pump performance,seven possible configurations have been considered(obtained by changing the blade wrap angle while keeping unchanged all the other parameters).Such configurations have been numerically simulated in the framework of a computational model based on the Reynolds time-averaged N-S equations,the RNG k-εturbulence approach and the SIMPLE algorithm.The impact exerted by different wrap angles of the guide vane on the performance of the pump,the internal losses of the guide vane and the flow field distribution in the bladeless area at the guide vane outlet has been assessed via cross-comparison of all these cases.The results show that the wrap angle has a significant influence:the wrap angle with the highest head is different from that with the highest efficiency,and changes in this angle have a more significant effect on the head than efficiency.A moderate raise of the wrap angle can improve the properties of the flow,reduce turbulence losses and enhance the energy conversion rate inside the guide vane.Different wrap angles can also lead to different fluid circulation modes in the bladeless area from guide vane outlet to impeller inlet,while they have a weak influence on the absolute value of the velocity of the fluid entering the impeller.
基金supported by the National Natural Science Foundation of China(No.51469013).
文摘The influence of the axial mount position of the guide vane on the pressure fluctuation in a nuclear pump(AP1000)is investigated.The characteristics of the three-dimensional flow inside the nuclear pump are analyzed by means of numerical simulation.Results indicate that when the axial relative distance between the guide vane and the pumping chamber is reduced,in conditions of“small flow,”the efficiency of the pump increases,the pressure inside the pumping chamber decreases,while the losses related to the guide vane grow.Under large flow conditions,as the efficiency of the pump decreases,the losses for the guide vane and the pumping chamber increase.The pressure fluctuation in the annular pumping chamber is basically determined by the rotation frequency and the blade passing frequency.The magnitude of these fluctuations is affected by the guide vane axial position.In particular,the smallest possible amplitude is obtained when the outlet central plane of the guide vane coincides with the outlet axis of the pumping chamber.
基金This work is supported by the National Natural Science Foundation of China(No.51469013).
文摘An AP1000 nuclear reactor coolant pump is considered to assess the influence of the Impeller/Guide vane clearance on the performances of this type of pumps.Experiments and numerical simulations relying on an unidirectional fluid-solid coupling approach are used to investigate the problem(stress,strain and mode of the rotor).The results reveal the relationship existing between the hydraulic performance of the nuclear reactor coolant pump and the clearance ratio.The effect of clearance ratio on the maximum equivalent stress on the back surface of the impeller blade is greater than that on the working surface(the maximum equivalent stress on the back surface of impeller blade is about three times that on the working surface).The clearance ratio has a scarce effect on the first six natural frequencies of the rotor of the nuclear reactor coolant pump.The related vibrational modes have different waveforms.
