Objective:To explore and validate the potential targets of Paeoniae Radix Alba(P.Radix,Bai Shao)in protecting against chemical liver injury through network pharmacology,molecular docking technology,and in vitro cell e...Objective:To explore and validate the potential targets of Paeoniae Radix Alba(P.Radix,Bai Shao)in protecting against chemical liver injury through network pharmacology,molecular docking technology,and in vitro cell experiments.Methods:Network pharmacology was used to identify the common potential targets of P.Radix and chemical liver injury.Molecular docking was used to fit the components,which were subsequently verified in vitro.A cell model of hepatic fibrosis was established by activating hepatic stellate cell(HSC)-LX2 cells with 10 ng/mL transforming growth factor-β1.The cells were exposed to different concentrations of total glucosides of paeony(TGP),the active substance of P.Radix,and then evaluated using the cell counting kit-8 assay,enzyme-linked immunosorbent assay,and western blot.Results:Analysis through network pharmacology revealed 13 key compounds of P.Radix,and the potential targets for preventing chemical liver injury were IL-6,AKT serine/threonine kinase 1,jun protooncogene,heat shock protein 90 alpha family class A member 1(HSP90AA1),peroxisome proliferator activated receptor gamma(PPARG),PTGS2,and CASP3.Gene Ontology(GO)enrichment analysis indicated the involvement of response to drugs,membrane rafts,and peptide binding.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis revealed that the main pathways involved lipid and atherosclerosis and chemical carcinogenesis-receptor activation.Paeoniflorin and albiflorin exhibited strong affinity for HSP90AA1,PTGS2,PPARG,and CASP3.Different concentrations of TGP can inhibit the expression of COL-I,COL-III,IL-6,TNF-a,IL-1β,HSP-90a,and PTGS2 while increasing the expression of PPAR-γand CASP3 in activated HSC-LX2 cells.Conclusion:P.Radix primarily can regulate targets such as HSP90AA1,PTGS2,PPARG,CASP3.TGP,the main active compound of P.Radix,protects against chemical liver injury by reducing the inflammatory response,activating apoptotic proteins,and promoting the apoptosis of activated HSCs.展开更多
Objective:To explore the mechanism of the Peiyuan Jieyu formula in treating depression by assessing its impact on a lipopolysaccharide-induced(LPS-induced)depression mouse model.Methods:We created a mouse model of dep...Objective:To explore the mechanism of the Peiyuan Jieyu formula in treating depression by assessing its impact on a lipopolysaccharide-induced(LPS-induced)depression mouse model.Methods:We created a mouse model of depression by exposing mice that had previously received chronic stress to intraperitoneal LPS injections.The mice were divided into the following groups:control,model,fluoxetine,Tiansi Yin,Sini powder,and low-,medium-,and high-dose Peiyuan Jieyu formula groups.Forced swim and tail suspension tests were used to assess the efficacy of the depression(despair)model,and weight gain rates were also measured.Furthermore,serum levels of various depression and inflammation-associated molecules,including tumor necrosis factor-a(TNF-a),interferon-γ(IFN-γ),tryptophan,5-hydroxytryptamine,kynurenine(KYN),and kynurenic acid(KA)were assessed.Furthermore,the expression levels of ionic calcium-binding adaptor molecule-1(IBA-1)and indoleamine 2,3-dioxygenase(IDO)mRNA in hippocampal microglia were measured.Results:The model group displayed greater despair-associated immobility,which was shortened in response to various doses of Peiyuan Jieyu formula.Furthermore,formula administration significantly reduced serum TNF-a levels and hippocampal IDO mRNA expression.The high formula dose also reduced IFN-γand IBA-1 levels,the latter was also decreased in response to the medium formula dose.However,the low formula dose reduced serum KYN level and KYN/tryptophan(TRP)and KYN/KA ratios.Conclusion:The Peiyuan Jieyu formula holds immense potential in treating depression in a mouse model,potentially inhibiting inflammation and improving TRP-KYN metabolic disorders.展开更多
Trimetallic palladium-copper-cobalt nanoparticles supported on reduced graphene oxide(PdCuCo/RGO)with different molar ratios of Pd,Cu and Co can be synthesized by facile chemical reduction with NaBH_4 as reductant and...Trimetallic palladium-copper-cobalt nanoparticles supported on reduced graphene oxide(PdCuCo/RGO)with different molar ratios of Pd,Cu and Co can be synthesized by facile chemical reduction with NaBH_4 as reductant and cetrimonium bromide as stabilizer.The morphology,structure and composition of the as-synthesized catalysts are characterized by transmission electron microscopy,X-ray diffraction and Xray photoelectron spectroscopy.The cyclic voltammetry and chronoamperometry are utilized to investigate the electrochemical activities and stabilities of the as-obtained catalysts.The results demonstrate that the PdCuCo/RGO catalyst shows superior catalytic activity and stability for methanol electrooxidation in alkaline media compared with PdCu/RGO,PdCo/RGO,and Pd/RGO catalysts.These findings suggest that the PdCuCo/RGO catalyst possesses a great potential as a promising anode catalyst for direct methanol fuel cells.展开更多
We assessed the eff ects of arbuscular mycorrhizal fungi(AMF)Rhizophagus irregularis inoculation on salt stress tolerance in roots of the drought-tolerant plant Elaeagnus angustifolia.We studied a plant growth index,s...We assessed the eff ects of arbuscular mycorrhizal fungi(AMF)Rhizophagus irregularis inoculation on salt stress tolerance in roots of the drought-tolerant plant Elaeagnus angustifolia.We studied a plant growth index,spore density and hyphal length density of AMF,the Na+contents and ultrastructure of root cells,as well as rhizosphere soil enzyme activities of mycorrhizal and non-mycorrhizal E.angustifolia seedlings under diff erent salt stress.Under salt stress,growth of E.angustifolia with mycorrhizal inoculation was higher than that of non-inoculated treatments.The spore density and hyphal length density decreased signifi cantly under salt stress in rhizosphere soil of mycorrhizal E.angustifolia seedlings(p<0.05).The root cells of E.angustifolia seedlings inoculated with R.irregularis at 300 mmol L−1 salt had more organelles,greater integrity,and lower root Na+contents than those of non-inoculated seedlings.In addition,the results showed notably higher activities of catalase,phosphatase,urease and saccharase in rhizosphere soil of the mycorrhizal seedlings in response to salinity compared to those of the non-mycorrhizal seedlings.Therefore,AMF inoculation could enhance salt stress tolerance in roots of E.angustifolia.展开更多
基金supported by the National Natural Science Foundation of China(82074036).
文摘Objective:To explore and validate the potential targets of Paeoniae Radix Alba(P.Radix,Bai Shao)in protecting against chemical liver injury through network pharmacology,molecular docking technology,and in vitro cell experiments.Methods:Network pharmacology was used to identify the common potential targets of P.Radix and chemical liver injury.Molecular docking was used to fit the components,which were subsequently verified in vitro.A cell model of hepatic fibrosis was established by activating hepatic stellate cell(HSC)-LX2 cells with 10 ng/mL transforming growth factor-β1.The cells were exposed to different concentrations of total glucosides of paeony(TGP),the active substance of P.Radix,and then evaluated using the cell counting kit-8 assay,enzyme-linked immunosorbent assay,and western blot.Results:Analysis through network pharmacology revealed 13 key compounds of P.Radix,and the potential targets for preventing chemical liver injury were IL-6,AKT serine/threonine kinase 1,jun protooncogene,heat shock protein 90 alpha family class A member 1(HSP90AA1),peroxisome proliferator activated receptor gamma(PPARG),PTGS2,and CASP3.Gene Ontology(GO)enrichment analysis indicated the involvement of response to drugs,membrane rafts,and peptide binding.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis revealed that the main pathways involved lipid and atherosclerosis and chemical carcinogenesis-receptor activation.Paeoniflorin and albiflorin exhibited strong affinity for HSP90AA1,PTGS2,PPARG,and CASP3.Different concentrations of TGP can inhibit the expression of COL-I,COL-III,IL-6,TNF-a,IL-1β,HSP-90a,and PTGS2 while increasing the expression of PPAR-γand CASP3 in activated HSC-LX2 cells.Conclusion:P.Radix primarily can regulate targets such as HSP90AA1,PTGS2,PPARG,CASP3.TGP,the main active compound of P.Radix,protects against chemical liver injury by reducing the inflammatory response,activating apoptotic proteins,and promoting the apoptosis of activated HSCs.
基金supported by the National Natural Science Foundation of China(81373584)。
文摘Objective:To explore the mechanism of the Peiyuan Jieyu formula in treating depression by assessing its impact on a lipopolysaccharide-induced(LPS-induced)depression mouse model.Methods:We created a mouse model of depression by exposing mice that had previously received chronic stress to intraperitoneal LPS injections.The mice were divided into the following groups:control,model,fluoxetine,Tiansi Yin,Sini powder,and low-,medium-,and high-dose Peiyuan Jieyu formula groups.Forced swim and tail suspension tests were used to assess the efficacy of the depression(despair)model,and weight gain rates were also measured.Furthermore,serum levels of various depression and inflammation-associated molecules,including tumor necrosis factor-a(TNF-a),interferon-γ(IFN-γ),tryptophan,5-hydroxytryptamine,kynurenine(KYN),and kynurenic acid(KA)were assessed.Furthermore,the expression levels of ionic calcium-binding adaptor molecule-1(IBA-1)and indoleamine 2,3-dioxygenase(IDO)mRNA in hippocampal microglia were measured.Results:The model group displayed greater despair-associated immobility,which was shortened in response to various doses of Peiyuan Jieyu formula.Furthermore,formula administration significantly reduced serum TNF-a levels and hippocampal IDO mRNA expression.The high formula dose also reduced IFN-γand IBA-1 levels,the latter was also decreased in response to the medium formula dose.However,the low formula dose reduced serum KYN level and KYN/tryptophan(TRP)and KYN/KA ratios.Conclusion:The Peiyuan Jieyu formula holds immense potential in treating depression in a mouse model,potentially inhibiting inflammation and improving TRP-KYN metabolic disorders.
基金supported by the Natural Science Foundation of China (Nos.21776302,21576289,and 21776308)the Science Foundation of China University of Petroleum,Beijing (Nos.2462017BJB04,2462015YQ0306,2462016YJRC027 and C201603)
文摘Trimetallic palladium-copper-cobalt nanoparticles supported on reduced graphene oxide(PdCuCo/RGO)with different molar ratios of Pd,Cu and Co can be synthesized by facile chemical reduction with NaBH_4 as reductant and cetrimonium bromide as stabilizer.The morphology,structure and composition of the as-synthesized catalysts are characterized by transmission electron microscopy,X-ray diffraction and Xray photoelectron spectroscopy.The cyclic voltammetry and chronoamperometry are utilized to investigate the electrochemical activities and stabilities of the as-obtained catalysts.The results demonstrate that the PdCuCo/RGO catalyst shows superior catalytic activity and stability for methanol electrooxidation in alkaline media compared with PdCu/RGO,PdCo/RGO,and Pd/RGO catalysts.These findings suggest that the PdCuCo/RGO catalyst possesses a great potential as a promising anode catalyst for direct methanol fuel cells.
基金This work was supported by Key Laboratory of Microbiology,College of Heilongjiang Province.We would also like to thank Elizabeth Tokarz at the Yale University for her assistance with English language and grammatical editing of the manuscript.
文摘We assessed the eff ects of arbuscular mycorrhizal fungi(AMF)Rhizophagus irregularis inoculation on salt stress tolerance in roots of the drought-tolerant plant Elaeagnus angustifolia.We studied a plant growth index,spore density and hyphal length density of AMF,the Na+contents and ultrastructure of root cells,as well as rhizosphere soil enzyme activities of mycorrhizal and non-mycorrhizal E.angustifolia seedlings under diff erent salt stress.Under salt stress,growth of E.angustifolia with mycorrhizal inoculation was higher than that of non-inoculated treatments.The spore density and hyphal length density decreased signifi cantly under salt stress in rhizosphere soil of mycorrhizal E.angustifolia seedlings(p<0.05).The root cells of E.angustifolia seedlings inoculated with R.irregularis at 300 mmol L−1 salt had more organelles,greater integrity,and lower root Na+contents than those of non-inoculated seedlings.In addition,the results showed notably higher activities of catalase,phosphatase,urease and saccharase in rhizosphere soil of the mycorrhizal seedlings in response to salinity compared to those of the non-mycorrhizal seedlings.Therefore,AMF inoculation could enhance salt stress tolerance in roots of E.angustifolia.
