Background:The active components of Horcha-6 were identified using liquid chromatography with tandem mass spectrometry.Also,we investigated the potential mechanisms that explain why Horcha-6 may be effective in treati...Background:The active components of Horcha-6 were identified using liquid chromatography with tandem mass spectrometry.Also,we investigated the potential mechanisms that explain why Horcha-6 may be effective in treating migraines through the use of network pharmacology and a rat migraine model.Methods:After identifying the active components of Horcha-6,the corresponding genes of the active components’target were obtained from the Universal Protein database,and a“compound-target-disease”network was constructed using Cytoscape 3.9.0 software.For the in vivo experiments,nitroglycerin was injected intraperitoneally into rats to create a migraine model.Pre-treatment with Horcha-6 was administered orally for 14 days,and rats were subjected to migraine-related behavior tests.RNA sequencing was performed to identify the gene expression regulated by Horcha-6 in the trigeminal nerve.Results:A total of 903 chemical components of Horcha-6 have been collected in the liquid chromatography with tandem mass spectrometry.We discovered 55 of the Horcha-6 bio-active components that were evaluated based on their Percent Human Oral Absorption(≥30%)and DL values(≥0.185)on the traditional Chinese medicine systems pharmacology database.The“compound-target-disease”network contained 163 intersection targets with the migraine state.Gene Ontology analysis indicated that these components significantly regulated the immune response,vascular function,oxidative stress,etc.When Kyoto Encyclopedia of Genes and Genomes enrichment analysis was performed,we observed that most of the target genes were significantly enriched in the inflammation and neuro-related signaling pathway,toll-like receptor signaling pathway,neuroactive ligand-receptor interaction,etc.These predictions were further demonstrated via in vivo animal model experiments.The RNA sequencing results showed that 41 genes were down-regulated(P<0.05)and 86 genes were up-regulated(P<0.05)in the Horcha-6 treated group compared with the untreated group.Those genes were mainly involved in neuromodulation,vascular function,and hormone metabolism.Conclusion:The 55 bio-active components in Horcha-6 regulate inflammation,hormone metabolism,and neurotransmitters and have potential as a therapy to treat migraines.展开更多
Ophiocordyceps sinensis is well known as a traditional Chinese medicine and has widely been used for over 2,000 years to stimulate immune system, decrease blood pressure and to inhibit tumor growth. While miRNAs are i...Ophiocordyceps sinensis is well known as a traditional Chinese medicine and has widely been used for over 2,000 years to stimulate immune system, decrease blood pressure and to inhibit tumor growth. While miRNAs are increasingly recognized for their roles in post-transcriptional regulation of gene expression in animals and plants, miRNAs in fungi were less studied until the discovery of microRNA-like RNA(milRNA). High-throughput sequencing and bioinformatics approaches were used to identify conserved and novel milRNAs in O. sinensis. 40 conserved milRNAs were identified, while 23 pre-miRNA candidates encoding 31 novel milRNAs were predicted. Furthermore, the potential target genes of milRNAs in human were predicted and gene ontology analysis was applied to these genes. Enrichment analysis of GO-represented biological process showed that target genes of both conserved and novel milRNAs are involved in development, metabolic and immune processes, indicating the potential roles of milRNAs of O. sinensis in pharmacological effects as health food and traditional Chinese medicine. This study is the first report on genome-wide analysis of milRNAs in O. sinensis and it provides a useful resource to further study the potential roles of milRNAs as active components of O. sinensis in health food or traditional Chinese medicine.展开更多
Thyroid disease is characterized by unusual levels of thyroid hormones,which results in either hyperthyroidism or hypothyroidism.The pathology of a particular type or stage of thyroid disease is very complicated,and a...Thyroid disease is characterized by unusual levels of thyroid hormones,which results in either hyperthyroidism or hypothyroidism.The pathology of a particular type or stage of thyroid disease is very complicated,and always linked to a variety of biological functions.Although the mortality rate is not high,thyroid dysfunction could lead to metabolic and immunological disorders that can subsequently cause discomfort.To date,many drugs are suggested to have curative effects on thyroid disease,however,drug toxicity and long treatment periods encourage the search for more promising ones.Prunella vulgaris L.(Labiatae)is a popular herb that has shown great potential for improving human immunity and organ protection.It has been extensively used in the treatment of many diseases but its ability to treat specific diseases has not been fully reported.In this review,a literature search regarding herbs and herbal recipes for treating thyroid disease were carried out,organized,and summarized.In addition,this study conducted a literature search on the current situation and progress of P.vulgaris treatment for various diseases.Finally,this study discussed studies regarding P.vulgaris treatment of goiter,and the mechanism of treatment through the regulation of apoptosis.Accordingly,a combination therapy of herbs and Western medicine can provide significant therapeutic effects in the clinical treatment of thyroid disease.Furthermore,the association between P.vulgaris and various diseases suggests that P.vulgaris is rich in a variety of active substances that can fight oxidation and participate in the regulation of apoptosis,thus having a protective effect on the thyroid.Here,a comprehensive literature review regarding the application of herbs or herbal recipes in the treatment of thyroid disease was presented.It is concluded that there is strong evidence for further research regarding the use of P.vulgaris in the treatment of thyroid diseases.展开更多
基金supported by grants The Natural Science Foundation of Inner Mongolia(2019MS08104)The Natural Science Foundation of Inner Mongolia(2022ZD09)The Central Government Guiding Special Funds for Development of Local Science and Technology(2020ZY0020).
文摘Background:The active components of Horcha-6 were identified using liquid chromatography with tandem mass spectrometry.Also,we investigated the potential mechanisms that explain why Horcha-6 may be effective in treating migraines through the use of network pharmacology and a rat migraine model.Methods:After identifying the active components of Horcha-6,the corresponding genes of the active components’target were obtained from the Universal Protein database,and a“compound-target-disease”network was constructed using Cytoscape 3.9.0 software.For the in vivo experiments,nitroglycerin was injected intraperitoneally into rats to create a migraine model.Pre-treatment with Horcha-6 was administered orally for 14 days,and rats were subjected to migraine-related behavior tests.RNA sequencing was performed to identify the gene expression regulated by Horcha-6 in the trigeminal nerve.Results:A total of 903 chemical components of Horcha-6 have been collected in the liquid chromatography with tandem mass spectrometry.We discovered 55 of the Horcha-6 bio-active components that were evaluated based on their Percent Human Oral Absorption(≥30%)and DL values(≥0.185)on the traditional Chinese medicine systems pharmacology database.The“compound-target-disease”network contained 163 intersection targets with the migraine state.Gene Ontology analysis indicated that these components significantly regulated the immune response,vascular function,oxidative stress,etc.When Kyoto Encyclopedia of Genes and Genomes enrichment analysis was performed,we observed that most of the target genes were significantly enriched in the inflammation and neuro-related signaling pathway,toll-like receptor signaling pathway,neuroactive ligand-receptor interaction,etc.These predictions were further demonstrated via in vivo animal model experiments.The RNA sequencing results showed that 41 genes were down-regulated(P<0.05)and 86 genes were up-regulated(P<0.05)in the Horcha-6 treated group compared with the untreated group.Those genes were mainly involved in neuromodulation,vascular function,and hormone metabolism.Conclusion:The 55 bio-active components in Horcha-6 regulate inflammation,hormone metabolism,and neurotransmitters and have potential as a therapy to treat migraines.
基金supported by the National Basic Research Program of China (2014CB542300)the National Natural Science Foundation of China (81602697)+1 种基金the Natural Science Foundation of Jiangsu Province (BE2016737) the Fundamental Research Funds for the Central Universities (020814380070)
文摘Ophiocordyceps sinensis is well known as a traditional Chinese medicine and has widely been used for over 2,000 years to stimulate immune system, decrease blood pressure and to inhibit tumor growth. While miRNAs are increasingly recognized for their roles in post-transcriptional regulation of gene expression in animals and plants, miRNAs in fungi were less studied until the discovery of microRNA-like RNA(milRNA). High-throughput sequencing and bioinformatics approaches were used to identify conserved and novel milRNAs in O. sinensis. 40 conserved milRNAs were identified, while 23 pre-miRNA candidates encoding 31 novel milRNAs were predicted. Furthermore, the potential target genes of milRNAs in human were predicted and gene ontology analysis was applied to these genes. Enrichment analysis of GO-represented biological process showed that target genes of both conserved and novel milRNAs are involved in development, metabolic and immune processes, indicating the potential roles of milRNAs of O. sinensis in pharmacological effects as health food and traditional Chinese medicine. This study is the first report on genome-wide analysis of milRNAs in O. sinensis and it provides a useful resource to further study the potential roles of milRNAs as active components of O. sinensis in health food or traditional Chinese medicine.
基金financially supported by the Inner Mongolia Natural Science Foundation Project (2020MS08203)the New drug Research&Development Projects of the Inner Mongolia Medical Research Institute (YJS20186 and 2016YJS21)
文摘Thyroid disease is characterized by unusual levels of thyroid hormones,which results in either hyperthyroidism or hypothyroidism.The pathology of a particular type or stage of thyroid disease is very complicated,and always linked to a variety of biological functions.Although the mortality rate is not high,thyroid dysfunction could lead to metabolic and immunological disorders that can subsequently cause discomfort.To date,many drugs are suggested to have curative effects on thyroid disease,however,drug toxicity and long treatment periods encourage the search for more promising ones.Prunella vulgaris L.(Labiatae)is a popular herb that has shown great potential for improving human immunity and organ protection.It has been extensively used in the treatment of many diseases but its ability to treat specific diseases has not been fully reported.In this review,a literature search regarding herbs and herbal recipes for treating thyroid disease were carried out,organized,and summarized.In addition,this study conducted a literature search on the current situation and progress of P.vulgaris treatment for various diseases.Finally,this study discussed studies regarding P.vulgaris treatment of goiter,and the mechanism of treatment through the regulation of apoptosis.Accordingly,a combination therapy of herbs and Western medicine can provide significant therapeutic effects in the clinical treatment of thyroid disease.Furthermore,the association between P.vulgaris and various diseases suggests that P.vulgaris is rich in a variety of active substances that can fight oxidation and participate in the regulation of apoptosis,thus having a protective effect on the thyroid.Here,a comprehensive literature review regarding the application of herbs or herbal recipes in the treatment of thyroid disease was presented.It is concluded that there is strong evidence for further research regarding the use of P.vulgaris in the treatment of thyroid diseases.
