Objective ：To elucidate whether cell multiplication, apoptosis, glucose intake and p-Akt protein expression of bone Mesenchyreal Stem Cells（MSCs） of rats is influenced by a hypoxic environment ex vivo. Methods ：Pa...Objective ：To elucidate whether cell multiplication, apoptosis, glucose intake and p-Akt protein expression of bone Mesenchyreal Stem Cells（MSCs） of rats is influenced by a hypoxic environment ex vivo. Methods ：Passage 3 of bone marrow MSCs taken from Wistar rats,were cultured in a culturing chamber with 94%N2,1%O2,5%CO2 at 37℃. At different hypoxia time points ,0,0.5, 1,4 and 8 h, glucose uptake was assayed by using radiation isotope ^3H-G, Apoptotic Rate（AR） and dead rate（DR） were analyzed by flow cytometry（FCM） after Annexin V/PI staining, cell multiplication（by MTr methods） and p-Akt protein by immunocytochemistry and western blot. Results ：Assay for CD29^± ,CD44^± ,CD71^± ,CD34^-, Tn T^±（after 5-azacytidine agent inducing） and ALP^±（after bone differentiation agent inducing） suggested these bone-derived cells were MSCs. The ^3H-G intaking ratio （CPM/ flask value：157 ± 11,110 ± 11,107 ± 13,103 ± 10,100 ± 9 and 98 ± 10） of MSCs at different hypoxia time points, significantly decreased compared to that of normoxia（P 〈 0.01） and tended to descend slowly with hypoxia time duration, for which there was no statistical significance（P 〉 0.05）. The AR（0.09 ± 2.03%,12.9 ± 1.72%,13.7 ± 2.26%,13.8 ± 3.01%,14.1 ± 2.78% and 14.7 ± 4.01% at 0,0.5,1,4 and 8 h,respectively,P 〈 0.01） and DR （0.04, ± 1.79% ,0.93 ± 1.85% ,3.11 ± 2.14% ,4.09 ± 2.36% ,4.72 ± 2.05% and 4.91 ± 3.72% at 0,0.5,1,4 and 8 h, respectively, P 〈 0.05） at different hypoxia time points significantly increased compared to those time in normoxia; The AR further went up with time （P 〈 0.05）, however there was no statistical significance （P 〉 0.05） for the DR. Optical absorption value of MTr methods at different hypoxia time points significantly decreased compared to those with a corresponding normoxia time （P 〈 0.01） and degraded with time （in an hypoxic environment -P 〈 0.01）. IOD of p-Akt protein of MSCs at different hypoxia time points significantly increased （0.367 ± 0.031,0.556 ± 0.023,0.579 ± 0.013, 0.660 ± 0.024, 0.685 ± 0.039 and 0.685 ± 0.011, respectively） compared to their equivalents in normoxia （P〈0.05）, however, there was no statistical significance （P 〉 0.05） for different hypoxia time points. Hypoxia may result in ultramicrostructure changes, such as defluvium of Microvilli, apoptotic body, ＂margination＂ and so on and are further aggravated with hypoxia time stretching. Conclusion： Hypoxia may lead to a depression of MSCs intaldng glucose, creep of cell multiplication, upregulation of p-Akt protein and apoptosis of MSCs ex vivo.展开更多
Unbalance existing in the mechanical systems is one of the most common causes which leads to unexpected vibration,nonsmooth motions,uncertain dynamics and even instability. In this paper,the problem of unbalance ident...Unbalance existing in the mechanical systems is one of the most common causes which leads to unexpected vibration,nonsmooth motions,uncertain dynamics and even instability. In this paper,the problem of unbalance identification and correction is investigated for the countershaft system of a precision centrifuge with two degrees of freedom. According to the characteristics of the load under test installed on the countershaft,a gradual subdivision algorithm is proposed to identify the phase of the unbalance,and its amplitude is calculated by using a space vector algorithm,where the vibration information of the mainshaft system is obtained by utilizing two axis-layout displacement transducers installed associated to the mainshaft.Based on ADAMS software,some numerical simulations are presented and compared,and further,the validity of the strategy is demonstrated by experimental examples.展开更多
In recent years,China's dry ports have entered into a period of rapid development,especially driven by the Belt and Road initiative(BRI).This initiative not only provides valuable opportunities,but also intensifie...In recent years,China's dry ports have entered into a period of rapid development,especially driven by the Belt and Road initiative(BRI).This initiative not only provides valuable opportunities,but also intensifies fierce competition between ports.In order to earn more profits and maintain an advantageous position,dry ports generally tend to rely on seaports to increase competitiveness and attract more goods,and seaports are also willing to cooperate with dry ports to expand their business.As a large coastal province in China,Shandong Province has good maritime resources.Because of the fierce competition among seaports inside and outside Shandong Province,port operators should make good use of the opportunity offered by BRI to make connections with the inland cities through the dry port-seaport logistics network and enhance the competitiveness.In order to take an active part in the process of the BRI,forming a dry port-seaport logistics network is a win-win strategy for Shandong province.The research first analyzes the impact of the Belt and Road initiative on ports of Shandong and its development,then uses complex network theory and TOPSIS method to select dry port candidate cities from BRI's important transportation nodes.After considering economic benefits,carbon emissions and construction costs,a multi-objective optimization model is established with a construction cost preference coefficient.Then the NSGA-II algorithm is used to solve the realistic problem.The study finds that when the construction cost increases,the transportation cost and carbon emission cost will decrease,which indicates that the dry port-seaport logistics network established under the BRI can reduce the cost of logistics transportation and environmental pollution.展开更多
文摘Objective ：To elucidate whether cell multiplication, apoptosis, glucose intake and p-Akt protein expression of bone Mesenchyreal Stem Cells（MSCs） of rats is influenced by a hypoxic environment ex vivo. Methods ：Passage 3 of bone marrow MSCs taken from Wistar rats,were cultured in a culturing chamber with 94%N2,1%O2,5%CO2 at 37℃. At different hypoxia time points ,0,0.5, 1,4 and 8 h, glucose uptake was assayed by using radiation isotope ^3H-G, Apoptotic Rate（AR） and dead rate（DR） were analyzed by flow cytometry（FCM） after Annexin V/PI staining, cell multiplication（by MTr methods） and p-Akt protein by immunocytochemistry and western blot. Results ：Assay for CD29^± ,CD44^± ,CD71^± ,CD34^-, Tn T^±（after 5-azacytidine agent inducing） and ALP^±（after bone differentiation agent inducing） suggested these bone-derived cells were MSCs. The ^3H-G intaking ratio （CPM/ flask value：157 ± 11,110 ± 11,107 ± 13,103 ± 10,100 ± 9 and 98 ± 10） of MSCs at different hypoxia time points, significantly decreased compared to that of normoxia（P 〈 0.01） and tended to descend slowly with hypoxia time duration, for which there was no statistical significance（P 〉 0.05）. The AR（0.09 ± 2.03%,12.9 ± 1.72%,13.7 ± 2.26%,13.8 ± 3.01%,14.1 ± 2.78% and 14.7 ± 4.01% at 0,0.5,1,4 and 8 h,respectively,P 〈 0.01） and DR （0.04, ± 1.79% ,0.93 ± 1.85% ,3.11 ± 2.14% ,4.09 ± 2.36% ,4.72 ± 2.05% and 4.91 ± 3.72% at 0,0.5,1,4 and 8 h, respectively, P 〈 0.05） at different hypoxia time points significantly increased compared to those time in normoxia; The AR further went up with time （P 〈 0.05）, however there was no statistical significance （P 〉 0.05） for the DR. Optical absorption value of MTr methods at different hypoxia time points significantly decreased compared to those with a corresponding normoxia time （P 〈 0.01） and degraded with time （in an hypoxic environment -P 〈 0.01）. IOD of p-Akt protein of MSCs at different hypoxia time points significantly increased （0.367 ± 0.031,0.556 ± 0.023,0.579 ± 0.013, 0.660 ± 0.024, 0.685 ± 0.039 and 0.685 ± 0.011, respectively） compared to their equivalents in normoxia （P〈0.05）, however, there was no statistical significance （P 〉 0.05） for different hypoxia time points. Hypoxia may result in ultramicrostructure changes, such as defluvium of Microvilli, apoptotic body, ＂margination＂ and so on and are further aggravated with hypoxia time stretching. Conclusion： Hypoxia may lead to a depression of MSCs intaldng glucose, creep of cell multiplication, upregulation of p-Akt protein and apoptosis of MSCs ex vivo.
基金Sponsored by the National Natural Science Foundation of China(Grant Nos.61203191 and 61427809)the China Postdoctoral Science Foundation(Grant No.2015M571415)+1 种基金the Heilongjiang Postdoctoral Foundation(Grant No.LBH-Z14088)the Fundamental Research Funds for the Central Universities(Grant No.HIT.NSRIF.201626)
文摘Unbalance existing in the mechanical systems is one of the most common causes which leads to unexpected vibration,nonsmooth motions,uncertain dynamics and even instability. In this paper,the problem of unbalance identification and correction is investigated for the countershaft system of a precision centrifuge with two degrees of freedom. According to the characteristics of the load under test installed on the countershaft,a gradual subdivision algorithm is proposed to identify the phase of the unbalance,and its amplitude is calculated by using a space vector algorithm,where the vibration information of the mainshaft system is obtained by utilizing two axis-layout displacement transducers installed associated to the mainshaft.Based on ADAMS software,some numerical simulations are presented and compared,and further,the validity of the strategy is demonstrated by experimental examples.
基金the National Natural Science Foundation of China(No.71772106)Humanities and Social Sciences Foundation of the Ministry of Education of China(No.l7YJCZH198)Shandong Provincial Natural Science Foundation,China(No.ZR2017MG012).
文摘In recent years,China's dry ports have entered into a period of rapid development,especially driven by the Belt and Road initiative(BRI).This initiative not only provides valuable opportunities,but also intensifies fierce competition between ports.In order to earn more profits and maintain an advantageous position,dry ports generally tend to rely on seaports to increase competitiveness and attract more goods,and seaports are also willing to cooperate with dry ports to expand their business.As a large coastal province in China,Shandong Province has good maritime resources.Because of the fierce competition among seaports inside and outside Shandong Province,port operators should make good use of the opportunity offered by BRI to make connections with the inland cities through the dry port-seaport logistics network and enhance the competitiveness.In order to take an active part in the process of the BRI,forming a dry port-seaport logistics network is a win-win strategy for Shandong province.The research first analyzes the impact of the Belt and Road initiative on ports of Shandong and its development,then uses complex network theory and TOPSIS method to select dry port candidate cities from BRI's important transportation nodes.After considering economic benefits,carbon emissions and construction costs,a multi-objective optimization model is established with a construction cost preference coefficient.Then the NSGA-II algorithm is used to solve the realistic problem.The study finds that when the construction cost increases,the transportation cost and carbon emission cost will decrease,which indicates that the dry port-seaport logistics network established under the BRI can reduce the cost of logistics transportation and environmental pollution.
