BACKGROUND The incidence of colon cancer is increasing worldwide.Treatments for colon cancer include surgery and surgery combined with chemotherapy and radiotherapy,but the median survival rate is still poor.Colon can...BACKGROUND The incidence of colon cancer is increasing worldwide.Treatments for colon cancer include surgery and surgery combined with chemotherapy and radiotherapy,but the median survival rate is still poor.Colon cancer most commonly metastasizes to the lymph nodes,lungs,liver,peritoneum,and brain,but breast metastasis is rare.There is no agreement on its treatment.CASE SUMMARY A 23-year-old woman was admitted to our hospital for further treatment with a history of acute abdominal pain,nausea,and vomiting.Her physical examination and computed tomography scan revealed an abdominal tumor.Transverse colectomy was successfully performed.Histopathological examination revealed that the tumor was a mucosecretory adenocarcinoma with signet ring cells.The patient inadvertently found a mass in the outer upper quadrant of the right breast after four cycles of XELOX chemotherapy[oxaliplatin 130 mg/m^(2),d1,intravenous(iv)drip for 2 h;capecitabine 1000 mg/m^(2),po,bid,d1–d14].After discussion with the patient,we performed a lumpectomy and frozen biopsy.The latter revealed that the breast tumor was intestinal metastasis.Genetic testing showed wild-type RAS and BRAF.So we replaced the original chemotherapy with FOLFIRI[irinotecan 180 mg/m^(2),d1,iv drip for 3–90 min;leucovorin 400 mg/m^(2),d1,iv drip for 2 h;5-fluorouracil(5-FU)400 mg/m^(2),d1 and 5-FU 1200 mg/(m^(2)d)×2 d,continuous iv drip for 46–48 h]+cetuximab(500 mg/m^(2),d1,iv drip for 2 h).Serum levels of tumor markers returned to normal after several treatment cycles,and there was no evidence of tumor recurrence or metastasis.CONCLUSION Breast metastasis from colon cancer is rare.Radical breast surgery should be avoided unless needed for palliation.Chemotherapy combined with targeted therapy should be the first choice.展开更多
BACKGROUND Cholangiocarcinoma and small intestine cancer are common clinical malignancies,but metastatic cholangiocarcinoma and small intestine cancer are rare,especially simultaneous metastatic cholangiocarcinoma and...BACKGROUND Cholangiocarcinoma and small intestine cancer are common clinical malignancies,but metastatic cholangiocarcinoma and small intestine cancer are rare,especially simultaneous metastatic cholangiocarcinoma and small intestine cancer from breast cancer.Since the clinical presentation of metastatic cholangiocarcinoma and small intestine cancer does not differ from primary tumor,it may lead to misdiagnosis preoperatively.CASE SUMMARY A 66-year-old woman was admitted to our hospital for further treatment due to abdominal pain and jaundice.Abdominal magnetic resonance imaging and magnetic resonance cholangiopancreatography showed an occupying lesion of the bile duct,considering a high possibility of primary bile duct tumor.Therefore,we performed a radical bile duct cancer surgery and cholecystectomy,and multiple tumors in the small intestine were found and removed during the surgery process.Postoperative pathology showed metastatic bile duct cancer and small intestine cancer from tumors in other parts.The patient underwent a right total mastectomy and axillary lymph node dissection because of right breast cancer 2 years ago.Combining with the immunohistochemical results,the patient was finally diagnosed as metastatic cholangiocarcinoma and metastatic small intestine cancer from breast cancer.Postoperatively,the patient received four cycles of chemotherapy and targeted therapy with docetaxel,capecitabine and trastuzumab.Unfortunately,the patient eventually died from tumor progression,thoracoabdominal infection,and sepsis 5 mo after surgery.CONCLUSION Simultaneous metastatic cholangiocarcinoma and small intestine cancer from breast cancer are rare and the prognosis is extremely poor.Improving preoperative diagnostic accuracy is beneficial to avoid excessive surgical treatment.Treatment should be aimed at relieving biliary obstruction and abdominal pain,and then supplemented with chemotherapy and targeted therapy to control tumor progression and prolong the patient’s life.展开更多
AIM:To analyze the expression of kallikrein gene 10(KLK10)in gastric cancer and to determine whether KLK10 has independent prognostic value in gastric cancer.METHODS:We studied KLK10 expression in 80 histologically co...AIM:To analyze the expression of kallikrein gene 10(KLK10)in gastric cancer and to determine whether KLK10 has independent prognostic value in gastric cancer.METHODS:We studied KLK10 expression in 80 histologically confirmed gastric cancer samples using realtime quantitative reverse transcription-PCR and hK10expression using immunohistochemistry.Correlations with clinicopathological variables(lymph node metastasis,depth of invasion and histology)and with outcomes(disease-free survival and overall survival)during a median follow-up period of 31 mo were assessed.Gastric cancer tissues were then classified as KLK10 positive or negative.RESULTS:KLK10 was found to be highly expressed in 57/80(70%)of gastric cancer samples,while its expression was very low in normal gastric tissues.Positive relationships between KLK10 expression and lymph node metastasis(P=0.048),depth of invasion(P=0.034)and histology(P=0.015)were observed.Univariate survival analysis revealed that gastric cancer patients with positive KLK10 expression had an increased risk for relapse/metastasis and death(P=0.005 and0.002,respectively).Cox multivariate analysis indicated that KLK10 was an independent prognostic indicator of disease-free survival and overall survival in patients with gastric cancer.CONCLUSION:KLK10 expression is an independent biomarker of unfavorable prognosis in patients with gastric cancer.展开更多
Poly methyl methacrylate(PMMA)bone cement is used in augmenting and stabilizing fractured vertebral bodies through percutaneous vertebroplasty(PVP)and percutaneous kyphoplasty(PKP).However,applications of PMMA bone ce...Poly methyl methacrylate(PMMA)bone cement is used in augmenting and stabilizing fractured vertebral bodies through percutaneous vertebroplasty(PVP)and percutaneous kyphoplasty(PKP).However,applications of PMMA bone cement are limited by the high elasticity modulus of PMMA,its low biodegradability,and its limited ability to regenerate bone.To improve PMMA bio activity and biodegradability and to modify its elasticity modulus,we mixed PMMA bone cement with oxidized hyaluronic acid and carboxymethyl chitosan in situ cross-linking hydrogel loaded with bone morphogenetic protein-2(BMP-2)to achieve novel hybrid cement.These fabric ated PMMA-hydrogel hybrid cements exhibited lower setting temperatures,a lower elasticity modulus,and better biodegradability and biocompatibility than that of pure PMMA cement,while retaining acceptable setting times,mechanical strength,and inj ectability.In addition,we detected release of BMP-2 from the PMMA-hydrogel hybrid cements,significantly enhancing in vitro osteogenesis of bone marrow mesenchymal stem cells by up-regulating the gene expression of Runx2,Coll,and OPN.Use of PMMA-hydrogel hybrid cements loaded with BMP-2 on rabbit femoral condyle bone-defect models revealed their biodegradability and enhanced bone formation.Our study demonstrated the favorable mechanical properties,biocompatibility,and biodegradability of fabricated PMMA-hydrogel hybrid cements loaded with BMP-2,as well as their ability to improve osteogenesis,making them a promising material for use in PKP and PVP.展开更多
Crosstalk between nerves and bone is essential for bone repair,for which Schwann cells(SCs)are crucial in the regulation of the microenvironment.Considering that exosomes are critical paracrine mediators for intercell...Crosstalk between nerves and bone is essential for bone repair,for which Schwann cells(SCs)are crucial in the regulation of the microenvironment.Considering that exosomes are critical paracrine mediators for intercellular communication that exert important effects in tissue repair,the aim of this study is to confirm the function and molecular mechanisms of Schwann cell-derived exosomes(SC-exos)on bone regeneration and to propose engineered constructs that simulate SC-mediated nerve-bone crosstalk.SCs promoted the proliferation and differentiation of bone marrow mesenchymal stem cells(BMSCs)through exosomes.Subsequent molecular mechanism studies demonstrated that SC-exos promoted BMSC osteogenesis by regulating the TGF-βsignaling pathway via let-7c-5p.Interestingly,SC-exos promoted the migration and tube formation performance of endothelial progenitor cells.Furthermore,the SC-exos@G/S constructs were developed by bioprinting technology that simulated SC-mediated nerve-bone crosstalk and improved the bone regeneration microenvironment by releasing SC-exos,exerting the regulatory effect of SCs in the microenvironment to promote innervation,vascularization,and osteogenesis and thus effectively improving bone repair in a cranial defect model.This study demonstrates the important role and underlying mechanism of SCs in regulating bone regeneration through SC-exos and provides a new engineered strategy for bone repair.展开更多
In the development of wearable energy devices,polypyrrole (PPy) is considered as a promising electrode material owing to its high capacitance and good mechanical flexibility.Herein,we report a PPy-based hybrid structu...In the development of wearable energy devices,polypyrrole (PPy) is considered as a promising electrode material owing to its high capacitance and good mechanical flexibility.Herein,we report a PPy-based hybrid structure consisting of vertical PPy nanotube arrays and carbon nano-onions (CNOs) grown on textile for wearable supercapacitors.In this hybrid nanostructure,the vertical PPy nanotubes provide straight and superhighways for electron and ion transport,boosting the energy storage;while the CNOs mainly act as a conductivity retainer for the underlayered PPy film during stretching.A facile template-degrading method is developed for the large-area growth of the PPy-based hybrid nanostructures on the textile through one-step polymerization process.The fabricated stretchable supercapacitor exhibits superior energy storage capacitance with the specific capacitance of 64 F·g^-1.Also,it presents the high capacitance retention of 99% at a strain of 50% after 500 stretching cycles.Furthermore,we demonstrate that the textile-based stretchable supercapacitor device can provide a stable energy storage performance in different wearable situations for practical applications.The use of the PPy-based hybrid nanostructures as the supercapacitor electrode offers a novel structure design and a promising opportunity for wearable power supply in real applications.展开更多
The Middle Permian Lucaogou Formation in the Jimusar Sag,eastern Junggar Basin,NW China,was deposited in a salt lake within an intracontinental rift basin with intense hydrothermal activity.Hydrothermal-sedimentary do...The Middle Permian Lucaogou Formation in the Jimusar Sag,eastern Junggar Basin,NW China,was deposited in a salt lake within an intracontinental rift basin with intense hydrothermal activity.Hydrothermal-sedimentary dolomite in the form of three types of dolostones,namely,analcime-feldspar dolostone(AFD),silicic dolostone(SD)and buddingtonite-albite dolostone(BAD),related to syn-sedimentary hydrothermal activity at lake bottom was discovered.The characteristics and formation mechanism of the dolomite were studied based on micron-scale petrographic and isotopic geochemical research.The syn-depositional formation of these dolostones was indicated by their rock-mineral features and syn-sedimentary deformation stage.The dolomite was composed of relatively poorly ordered proto-dolomite crystals with micron-sized spherical or sub-spherical morphology and coexisted with hydrothermal minerals,including analcime,buddingtonite,albite and chalcedony.Albite clasts were replaced by the dolomite,indicating high-temperature conditions during formation.The remarkably low strontium isotopic compositions of the dolostones(87Sr/86Sr with an average of 0.705687)indicated that mantle-derived materials might have involved in the ore-forming fluid.The dolostones had positiveδ13CPDB values(with an average of 6.94‰)and negativeδ18OPDB values(with an average of-8.12‰).Based on theδ18OPDB values,the formation temperatures of the dolomite were at least^25ºC higher than those of the penecontemporaneous dolomite in the Lucaogou Formation in the study area.It is concluded that the dolomite precipitated from hydrothermal fluid erupting at the lake bottom.The possible genetic models are described.We suggest that the hydrothermal-sedimentary dolomite is an important genetic type,and this study may help increase the awareness of this understudied type of dolomite.展开更多
Objective 14-3-3-zeta protein has been found to be associated with survival signaling in cancer.However,prognostic value and the predictive effect of its gene expression for determining the efficacy of endocrine thera...Objective 14-3-3-zeta protein has been found to be associated with survival signaling in cancer.However,prognostic value and the predictive effect of its gene expression for determining the efficacy of endocrine therapy in breast cancer are unclear.Methods The differential 14-3-3-zeta gene expression between cancer and normal tissue was assayed using ONCOMINE database analysis.The correlation between 14-3-3-zeta gene and proliferative/metastasis-associated genes was analyzed using Breast Cancer Gene-Expression Miner v4.1(bc-Gen Ex Miner v4.1).The prognostic value of its expression in breast cancer was analyzed using bc-Gen Ex Miner v4.1 and Kaplan-Meier Plotter.Results The 14-3-3-zeta gene expression was elevated in breast cancer tissue compared with normal breast tissue,which was higher in invasive ductal breast cancer than in ductal breast cancer in situ.It was also positively correlated with the degree of malignancy and the clinical stage of breast cancer and with some proliferative genes.A high level of 14-3-3-zeta expression was predictive of shorter relapse-free survival(RFS)in ER-positive but not ER-negative breast cancer.Further analysis showed the association of high 14-3-3-zeta expression and a shorter RFS in endocrine therapy or tamoxifen-only-treated population,regardless of whether chemotherapy had been used.Conclusion 14-3-3-zeta gene expression is upregulated and associated with a relatively high degree of malignancy and late clinical stage in breast cancer.It is a promising prognostic factor for ER-positive breast cancer and a predictor of the efficacy of endocrine therapy.展开更多
文摘BACKGROUND The incidence of colon cancer is increasing worldwide.Treatments for colon cancer include surgery and surgery combined with chemotherapy and radiotherapy,but the median survival rate is still poor.Colon cancer most commonly metastasizes to the lymph nodes,lungs,liver,peritoneum,and brain,but breast metastasis is rare.There is no agreement on its treatment.CASE SUMMARY A 23-year-old woman was admitted to our hospital for further treatment with a history of acute abdominal pain,nausea,and vomiting.Her physical examination and computed tomography scan revealed an abdominal tumor.Transverse colectomy was successfully performed.Histopathological examination revealed that the tumor was a mucosecretory adenocarcinoma with signet ring cells.The patient inadvertently found a mass in the outer upper quadrant of the right breast after four cycles of XELOX chemotherapy[oxaliplatin 130 mg/m^(2),d1,intravenous(iv)drip for 2 h;capecitabine 1000 mg/m^(2),po,bid,d1–d14].After discussion with the patient,we performed a lumpectomy and frozen biopsy.The latter revealed that the breast tumor was intestinal metastasis.Genetic testing showed wild-type RAS and BRAF.So we replaced the original chemotherapy with FOLFIRI[irinotecan 180 mg/m^(2),d1,iv drip for 3–90 min;leucovorin 400 mg/m^(2),d1,iv drip for 2 h;5-fluorouracil(5-FU)400 mg/m^(2),d1 and 5-FU 1200 mg/(m^(2)d)×2 d,continuous iv drip for 46–48 h]+cetuximab(500 mg/m^(2),d1,iv drip for 2 h).Serum levels of tumor markers returned to normal after several treatment cycles,and there was no evidence of tumor recurrence or metastasis.CONCLUSION Breast metastasis from colon cancer is rare.Radical breast surgery should be avoided unless needed for palliation.Chemotherapy combined with targeted therapy should be the first choice.
文摘BACKGROUND Cholangiocarcinoma and small intestine cancer are common clinical malignancies,but metastatic cholangiocarcinoma and small intestine cancer are rare,especially simultaneous metastatic cholangiocarcinoma and small intestine cancer from breast cancer.Since the clinical presentation of metastatic cholangiocarcinoma and small intestine cancer does not differ from primary tumor,it may lead to misdiagnosis preoperatively.CASE SUMMARY A 66-year-old woman was admitted to our hospital for further treatment due to abdominal pain and jaundice.Abdominal magnetic resonance imaging and magnetic resonance cholangiopancreatography showed an occupying lesion of the bile duct,considering a high possibility of primary bile duct tumor.Therefore,we performed a radical bile duct cancer surgery and cholecystectomy,and multiple tumors in the small intestine were found and removed during the surgery process.Postoperative pathology showed metastatic bile duct cancer and small intestine cancer from tumors in other parts.The patient underwent a right total mastectomy and axillary lymph node dissection because of right breast cancer 2 years ago.Combining with the immunohistochemical results,the patient was finally diagnosed as metastatic cholangiocarcinoma and metastatic small intestine cancer from breast cancer.Postoperatively,the patient received four cycles of chemotherapy and targeted therapy with docetaxel,capecitabine and trastuzumab.Unfortunately,the patient eventually died from tumor progression,thoracoabdominal infection,and sepsis 5 mo after surgery.CONCLUSION Simultaneous metastatic cholangiocarcinoma and small intestine cancer from breast cancer are rare and the prognosis is extremely poor.Improving preoperative diagnostic accuracy is beneficial to avoid excessive surgical treatment.Treatment should be aimed at relieving biliary obstruction and abdominal pain,and then supplemented with chemotherapy and targeted therapy to control tumor progression and prolong the patient’s life.
文摘AIM:To analyze the expression of kallikrein gene 10(KLK10)in gastric cancer and to determine whether KLK10 has independent prognostic value in gastric cancer.METHODS:We studied KLK10 expression in 80 histologically confirmed gastric cancer samples using realtime quantitative reverse transcription-PCR and hK10expression using immunohistochemistry.Correlations with clinicopathological variables(lymph node metastasis,depth of invasion and histology)and with outcomes(disease-free survival and overall survival)during a median follow-up period of 31 mo were assessed.Gastric cancer tissues were then classified as KLK10 positive or negative.RESULTS:KLK10 was found to be highly expressed in 57/80(70%)of gastric cancer samples,while its expression was very low in normal gastric tissues.Positive relationships between KLK10 expression and lymph node metastasis(P=0.048),depth of invasion(P=0.034)and histology(P=0.015)were observed.Univariate survival analysis revealed that gastric cancer patients with positive KLK10 expression had an increased risk for relapse/metastasis and death(P=0.005 and0.002,respectively).Cox multivariate analysis indicated that KLK10 was an independent prognostic indicator of disease-free survival and overall survival in patients with gastric cancer.CONCLUSION:KLK10 expression is an independent biomarker of unfavorable prognosis in patients with gastric cancer.
基金supported by the National Key R&D Program of China(No.2018YFA0703000)the National Natural Science Foundation of China(Nos.82071564,82072412,and 81772326)+1 种基金the Fundamental Research Program Funding of Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine(No.JYZZ070)Project of Shanghai Science and Technology Commission(No.19XD1434200/18431903700)。
文摘Poly methyl methacrylate(PMMA)bone cement is used in augmenting and stabilizing fractured vertebral bodies through percutaneous vertebroplasty(PVP)and percutaneous kyphoplasty(PKP).However,applications of PMMA bone cement are limited by the high elasticity modulus of PMMA,its low biodegradability,and its limited ability to regenerate bone.To improve PMMA bio activity and biodegradability and to modify its elasticity modulus,we mixed PMMA bone cement with oxidized hyaluronic acid and carboxymethyl chitosan in situ cross-linking hydrogel loaded with bone morphogenetic protein-2(BMP-2)to achieve novel hybrid cement.These fabric ated PMMA-hydrogel hybrid cements exhibited lower setting temperatures,a lower elasticity modulus,and better biodegradability and biocompatibility than that of pure PMMA cement,while retaining acceptable setting times,mechanical strength,and inj ectability.In addition,we detected release of BMP-2 from the PMMA-hydrogel hybrid cements,significantly enhancing in vitro osteogenesis of bone marrow mesenchymal stem cells by up-regulating the gene expression of Runx2,Coll,and OPN.Use of PMMA-hydrogel hybrid cements loaded with BMP-2 on rabbit femoral condyle bone-defect models revealed their biodegradability and enhanced bone formation.Our study demonstrated the favorable mechanical properties,biocompatibility,and biodegradability of fabricated PMMA-hydrogel hybrid cements loaded with BMP-2,as well as their ability to improve osteogenesis,making them a promising material for use in PKP and PVP.
基金support from National Key R&D Program of China(2018YFA0703000)National Natural Science Foundation of China(82072412)+1 种基金Translation Medicine National Key Science and Technology Infrastructure(Shanghai)Open Project(TMSK-2020-118)Lingang Laboratory“Seeking Outstanding Youth Program”open project(LG-QS-202206-04).
文摘Crosstalk between nerves and bone is essential for bone repair,for which Schwann cells(SCs)are crucial in the regulation of the microenvironment.Considering that exosomes are critical paracrine mediators for intercellular communication that exert important effects in tissue repair,the aim of this study is to confirm the function and molecular mechanisms of Schwann cell-derived exosomes(SC-exos)on bone regeneration and to propose engineered constructs that simulate SC-mediated nerve-bone crosstalk.SCs promoted the proliferation and differentiation of bone marrow mesenchymal stem cells(BMSCs)through exosomes.Subsequent molecular mechanism studies demonstrated that SC-exos promoted BMSC osteogenesis by regulating the TGF-βsignaling pathway via let-7c-5p.Interestingly,SC-exos promoted the migration and tube formation performance of endothelial progenitor cells.Furthermore,the SC-exos@G/S constructs were developed by bioprinting technology that simulated SC-mediated nerve-bone crosstalk and improved the bone regeneration microenvironment by releasing SC-exos,exerting the regulatory effect of SCs in the microenvironment to promote innervation,vascularization,and osteogenesis and thus effectively improving bone repair in a cranial defect model.This study demonstrates the important role and underlying mechanism of SCs in regulating bone regeneration through SC-exos and provides a new engineered strategy for bone repair.
基金The Science and Technology Foundation of Shenyang City(Grant No.RC170545)the Science and Technology Foundation of Liaoning Province(Grant No.20170540995)the National Science Foundation of China(Grant No.81302313)
基金the National Natural Science Foundation of China (No.51702233)the Natural Science Foundation of Tianjin City (No.16JCYBJC41000)support by Tianjin Key Subject for Materials Physics and Chemistry.
文摘In the development of wearable energy devices,polypyrrole (PPy) is considered as a promising electrode material owing to its high capacitance and good mechanical flexibility.Herein,we report a PPy-based hybrid structure consisting of vertical PPy nanotube arrays and carbon nano-onions (CNOs) grown on textile for wearable supercapacitors.In this hybrid nanostructure,the vertical PPy nanotubes provide straight and superhighways for electron and ion transport,boosting the energy storage;while the CNOs mainly act as a conductivity retainer for the underlayered PPy film during stretching.A facile template-degrading method is developed for the large-area growth of the PPy-based hybrid nanostructures on the textile through one-step polymerization process.The fabricated stretchable supercapacitor exhibits superior energy storage capacitance with the specific capacitance of 64 F·g^-1.Also,it presents the high capacitance retention of 99% at a strain of 50% after 500 stretching cycles.Furthermore,we demonstrate that the textile-based stretchable supercapacitor device can provide a stable energy storage performance in different wearable situations for practical applications.The use of the PPy-based hybrid nanostructures as the supercapacitor electrode offers a novel structure design and a promising opportunity for wearable power supply in real applications.
基金This study was supported by the National Natural Science Foundation of China(Grant Nos.41572086,41802120).
文摘The Middle Permian Lucaogou Formation in the Jimusar Sag,eastern Junggar Basin,NW China,was deposited in a salt lake within an intracontinental rift basin with intense hydrothermal activity.Hydrothermal-sedimentary dolomite in the form of three types of dolostones,namely,analcime-feldspar dolostone(AFD),silicic dolostone(SD)and buddingtonite-albite dolostone(BAD),related to syn-sedimentary hydrothermal activity at lake bottom was discovered.The characteristics and formation mechanism of the dolomite were studied based on micron-scale petrographic and isotopic geochemical research.The syn-depositional formation of these dolostones was indicated by their rock-mineral features and syn-sedimentary deformation stage.The dolomite was composed of relatively poorly ordered proto-dolomite crystals with micron-sized spherical or sub-spherical morphology and coexisted with hydrothermal minerals,including analcime,buddingtonite,albite and chalcedony.Albite clasts were replaced by the dolomite,indicating high-temperature conditions during formation.The remarkably low strontium isotopic compositions of the dolostones(87Sr/86Sr with an average of 0.705687)indicated that mantle-derived materials might have involved in the ore-forming fluid.The dolostones had positiveδ13CPDB values(with an average of 6.94‰)and negativeδ18OPDB values(with an average of-8.12‰).Based on theδ18OPDB values,the formation temperatures of the dolomite were at least^25ºC higher than those of the penecontemporaneous dolomite in the Lucaogou Formation in the study area.It is concluded that the dolomite precipitated from hydrothermal fluid erupting at the lake bottom.The possible genetic models are described.We suggest that the hydrothermal-sedimentary dolomite is an important genetic type,and this study may help increase the awareness of this understudied type of dolomite.
基金Science and Technology Foundation of Liaoning Provincegrant number:20170540995+5 种基金Science and Technology Foundation of Shenyang Citygrant number:RC170545the National Science Foundation of Chinagrant number:81302313Talent Project of Shengjing Hospital of China Medical Universitygrant number:345
文摘Objective 14-3-3-zeta protein has been found to be associated with survival signaling in cancer.However,prognostic value and the predictive effect of its gene expression for determining the efficacy of endocrine therapy in breast cancer are unclear.Methods The differential 14-3-3-zeta gene expression between cancer and normal tissue was assayed using ONCOMINE database analysis.The correlation between 14-3-3-zeta gene and proliferative/metastasis-associated genes was analyzed using Breast Cancer Gene-Expression Miner v4.1(bc-Gen Ex Miner v4.1).The prognostic value of its expression in breast cancer was analyzed using bc-Gen Ex Miner v4.1 and Kaplan-Meier Plotter.Results The 14-3-3-zeta gene expression was elevated in breast cancer tissue compared with normal breast tissue,which was higher in invasive ductal breast cancer than in ductal breast cancer in situ.It was also positively correlated with the degree of malignancy and the clinical stage of breast cancer and with some proliferative genes.A high level of 14-3-3-zeta expression was predictive of shorter relapse-free survival(RFS)in ER-positive but not ER-negative breast cancer.Further analysis showed the association of high 14-3-3-zeta expression and a shorter RFS in endocrine therapy or tamoxifen-only-treated population,regardless of whether chemotherapy had been used.Conclusion 14-3-3-zeta gene expression is upregulated and associated with a relatively high degree of malignancy and late clinical stage in breast cancer.It is a promising prognostic factor for ER-positive breast cancer and a predictor of the efficacy of endocrine therapy.
