Objective:To explore the efficacy and potential mechanisms of the ethanol extract of Abelmoschus manihot(L.)Medic in contrast-induced nephropathy(CIN).Methods:CIN rat models and human renal proximal tubular cells(HK-2...Objective:To explore the efficacy and potential mechanisms of the ethanol extract of Abelmoschus manihot(L.)Medic in contrast-induced nephropathy(CIN).Methods:CIN rat models and human renal proximal tubular cells(HK-2)with iopromide-induced injury were employed to mimic CIN conditions.The effect of Abelmoschus manihot extract on the rat models and HK-2 cells was evaluated.In rat models,kidney function,histology,oxidative stress and apoptosis were determined.In HK-2 cells,cell viability,apoptosis,mitochondrial membrane potential,and endoplasmic reticulum stress were assessed.Results:Abelmoschus manihot extract significantly improved structural and functional impairments in the kidneys of CIN rats.Additionally,the extract effectively mitigated the decline in cellular viability and reduced iopromide-induced oxidative stress and lipid peroxidation.Mechanistic investigations revealed that Abelmoschus manihot extract prominently attenuated acute endoplasmic reticulum stress-mediated apoptosis by downregulating GRP78 and CHOP protein levels.Conclusions:Abelmoschus manihot extract can be used as a promising therapeutic and preventive agent in the treatment of CIN.展开更多
BACKGROUND:Sepsis-associated encephalopathy(SAE) is a critical disease caused by sepsis.In addition to high mortality,SAE can also adversely aff ect life quality and lead to significant socioeconomic costs.This review...BACKGROUND:Sepsis-associated encephalopathy(SAE) is a critical disease caused by sepsis.In addition to high mortality,SAE can also adversely aff ect life quality and lead to significant socioeconomic costs.This review aims to explore the development of evaluation animal models of SAE,giving insight into the direction of future research in terms of its pathophysiology and therapy.METHODS:We performed a literature search from January 1,2000,to December 31,2022,in MEDLINE,PubMed,EMBASE,and Web of Science using related keywords.Two independent researchers screened all the accessible articles based on the inclusion and exclusion criteria and collected the relevant data of the studies.RESULTS:The animal models for sepsis are commonly induced through cecal ligation and puncture(CLP) or lipopolysaccharide(LPS) injection.SAE can be evaluated using nervous reflex scores and sepsis evaluation during the acute phase,or through Morris water maze(MWM),openfield test,fear condition(FC) test,inhibitory avoidance,and other tests during the late phase.CONCLUSION:CLP and LPS injection are the most common methods for establishing SAE animal models.Nervous reflexs cores,MWM,FC test,and inhibitory avoidance are widely used in SAE model analysis.Future research should focus on establishing a standardized system for SAE development and analysis.展开更多
AIM:To analyze the expression profiles of long noncoding RNAs(lncRNAs)in hepatocellular carcinoma.METHODS:Hepatocellular carcinoma(HCC)tissues and matched adjacent non-tumor(NT)liver tissues were collected from 29 pat...AIM:To analyze the expression profiles of long noncoding RNAs(lncRNAs)in hepatocellular carcinoma.METHODS:Hepatocellular carcinoma(HCC)tissues and matched adjacent non-tumor(NT)liver tissues were collected from 29 patients with HCC,immediately after liver resection,between March 2011 and July2013.The diagnosis of HCC was made based on histological examination.Differentially expressed lncRNAs between HCC and NT tissues were revealed through microarray-based lncRNAs expression profiling.Further,quantification of selected lncRNAs was performed using quantitative real-time reverse transcription polymerase chain reaction(qRT-PCR).RESULTS:Six hundred and fifty-nine lncRNAs were differentially expressed between HCC and NT tissues,of which five[TCONS_00018278,AK093543,D16366,ENST00000501583,NR_002819(MALAT1)]were selected for validation.Four of them were significantly downregulated in HCC tissues compared with NT tissues(P=0.012,0.045,0.000 and 0.000,respectively),and the expression level of MALAT1 showed no significant difference(P=0.114).CONCLUSION:This study identified a set of lncRNAs differentially expressed in HCC tissues and provided useful information for exploring potential therapeutic targets and diagnostic biomarkers of this cancer.展开更多
BACKGROUND: Golgi protein 73(GP73) is a promising biomarker of hepatocellular carcinoma(HCC). It decreases after surgical resection, and resumes upon recurrence, indicating a potential indicator for the effectiveness ...BACKGROUND: Golgi protein 73(GP73) is a promising biomarker of hepatocellular carcinoma(HCC). It decreases after surgical resection, and resumes upon recurrence, indicating a potential indicator for the effectiveness of the treatment. But changes of GP73 after transcatheter arterial chemoembolization(TACE) have not been reported so far. This study was to investigate the dynamic changes of GP73 in HCC patients after TACE treatment, and the possible underlying mechanisms in the cell cultures.METHODS: Blood samples were collected from 72 HCC patients, before TACE, at day 1 and day 30 after TACE. GP73 levels were measured by Western blotting. The dynamic changes of GP73 were analyzed and compared with image changes and clinical data. The effects of chemotherapeutic agents(5-FU and pirarubicin) on GP73 expression were tested in three HCC cell lines(Hep G2, HCCLM3 and MHCC97H).RESULTS: The GP73 level was significantly elevated at day 1and day 30 after TACE in HCC patients compared with that before the procedure(P<0.05). There was no statistical difference between the two time points after TACE, nor correlationbetween GP73 levels and clinicopathological features, tumor metastasis, and patient survival. Pirarubicin, not 5-FU, significantly increased GP73 expression in three cell lines. CONCLUSIONS: Unlike surgical resection which decreases the GP73 level, TACE significantly increased GP73 expression in patients with HCC. No correlations were observed among GP73 levels, tumor characteristics and prognosis of patients with HCC.展开更多
BACKGROUND Gallbladder carcinoma(GBC)carries a poor prognosis and requires a prediction method.Gamma-glutamyl transferase–to–platelet ratio(GPR)is a recently reported cancer prognostic factor.Although the mechanism ...BACKGROUND Gallbladder carcinoma(GBC)carries a poor prognosis and requires a prediction method.Gamma-glutamyl transferase–to–platelet ratio(GPR)is a recently reported cancer prognostic factor.Although the mechanism for the relationship between GPR and poor cancer prognosis remains unclear,studies have demonstrated the clinical effect of both gamma-glutamyl transferase and platelet count on GBC and related gallbladder diseases.AIM To assess the prognostic value of GPR and to design a prognostic nomogram for GBC.METHODS The analysis involved 130 GBC patients who underwent surgery at Peking Union Medical College Hospital from December 2003 to April 2017.The patients were stratified into a high-or low-GPR group.The predictive ability of GPR was evaluated by Kaplan–Meier analysis and a Cox regression model.We developed a nomogram based on GPR,which we verified using calibration curves.The nomogram and other prognosis prediction models were compared using timedependent receiver operating characteristic curves and the concordance index.RESULTS Patients in the high-GPR group had a higher risk of jaundice,were older,and had higher carbohydrate antigen 19-9 levels and worse postoperative outcomes.Univariate analysis revealed that GPR,age,body mass index,tumor–node–metastasis(TNM)stage,jaundice,cancer cell differentiation degree,and carcinoembryonic antigen and carbohydrate antigen 19-9 levels were related to overall survival(OS).Multivariate analysis confirmed that GPR,body mass index,age,and TNM stage were independent predictors of poor OS.Calibration curves were highly consistent with actual observations.Comparisons of timedependent receiver operating characteristic curves and the concordance index showed advantages for the nomogram over TNM staging.CONCLUSION GPR is an independent predictor of GBC prognosis,and nomogram-integrated GPR is a promising predictive model for OS in GBC.展开更多
Nb-based powder was fabricated via mechanical grinding. The influence of stearic acid on the grinding process was studied. The structural evolution and morphological evolution of the milled powder were characterized b...Nb-based powder was fabricated via mechanical grinding. The influence of stearic acid on the grinding process was studied. The structural evolution and morphological evolution of the milled powder were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS) analysis. It is indicated that an appropriate amount of stearic acid accelerates the particle refinement process and favors the production of superfine Nb-based particles with good dispersivity and high activity. However, an inappropriate amount of stearic acid has an adverse effect on the refinement process.展开更多
AIM To investigate the evaluation of neogalactosylalbumin(NGA) for liver function assessment based on positron emission tomography technology.METHODS Female Kunming mice were assigned randomly to two groups: fibrosis ...AIM To investigate the evaluation of neogalactosylalbumin(NGA) for liver function assessment based on positron emission tomography technology.METHODS Female Kunming mice were assigned randomly to two groups: fibrosis group and normal control group. A murine hepatic fibrosis model was generated by intraperitoneal injection of 10% carbon tetrachloride(CCl_4) at 0.4 m L every 48 h for 42 d. ^(18)F-labeled NGA([^(18)F]FNGA) was synthesized and administered at a dosage of 3.7 MBq/mouse to both fibrosis mice and normal control mice. Distribution of [^(18)F]FNGA amongst organs was examined, and dynamic scanning was performed. Parameters were set up to compare the uptake of tracers by fibrotic liver and healthy liver. Serologic tests for liver function were also performed.RESULTS The liver function of the fibrosis model mice was significantly impaired by the use of CCl_4. In the fibrosis model mice, hepatic fibrosis was verified by naked eye assessment and pathological analysis. [^(18)F]FNGA was found to predominantly accumulate in liver and kidneys in both control group(n = 21) and fibrosis group(n = 23). The liver uptake ability(LUA), peak time(T_p), and uptake rate(LUR) of [^(18)F]FNGA between healthy liver(n = 8) and fibrosis liver(n = 10) were significantly different(P < 0.05, < 0.01, and < 0.05, respectively). LUA was significantly correlated with total serum protein level(TP)(P < 0.05). T_p was significantly correlated with both TP and glucose(Glu) concentration(P < 0.05 both), and LUR was significantly correlated with both total bile acid and Glu concentration(P < 0.01 and < 0.05, respectively).CONCLUSION[^(18)F]FNGA mainly accumulated in liver and remained for sufficient time. Functionally-impaired liver showed a significant different uptake pattern of [^(18)F]FNGA compared to the controls.展开更多
Objective: To study the effects of astragalus polysaccharide on cell injury and mitochondrial pathway apoptosis in the hypoxia reoxygenation of myocardial cells. Methods: H9c2 myocardial cell lines were cultured and d...Objective: To study the effects of astragalus polysaccharide on cell injury and mitochondrial pathway apoptosis in the hypoxia reoxygenation of myocardial cells. Methods: H9c2 myocardial cell lines were cultured and divided into negative control group (NC group), hypoxia reoxygenation group (H/R group) and astragalus polysaccharide group (APS), H/R group underwent hypoxia reoxygenation treatment, and APS group underwent both hypoxia reoxygenation treatment and astragalus polysaccharides intervention. The cell viability was measured 8 h, 16 h and 24 h after reoxygenation;the expression of mitochondrial apoptosis genes, apoptosis pathway genes as well as the contents of ROS metabolism indexes were determined 24 h after reoxygenation. Results: 8 h, 16 h and 24 h after reoxygenation, the cell viability of H/R group were lower than those of NC group, and the cell viability of APS group were higher than those of H/R group;24 h after reoxygenation, BIM, BAX, Caspase-9, Caspase-3, p-PI3K, p-AKT and p-FoxO3a protein expression as well as ROS, HSP70 and p-p38MAPK contents in H/R group were significantly higher than those in NC group whereas BCL2 protein expression and SOD content were significantly lower than those in NC group;BIM, BAX, Caspase-9, Caspase-3, p-PI3K, p-AKT and p-FoxO3a protein expression as well as ROS, HSP70 and p-p38MAPK contents in APS group were significantly lower than those in H/R group whereas BCL2 protein expression and SOD content were significantly higher than those in H/R group. Conclusion: Astragalus polysaccharide can reduce the cell damage and inhibit the mitochondrial pathway apoptosis in the hypoxia reoxygenation process of myocardial cells.展开更多
The evolution of wearable technology has prompted the need for adaptive,self-healable,and energy-autonomous energy devices.This study innovatively addresses this challenge by introducing an MXene-boosted hydrogel elec...The evolution of wearable technology has prompted the need for adaptive,self-healable,and energy-autonomous energy devices.This study innovatively addresses this challenge by introducing an MXene-boosted hydrogel electrolyte,which expedites the assembly process of flexible thermocell(TEC)arrays and thus circumvents the complicated fabrication of typical wearable electronics.Our findings underscore the hydrogel electrolyte’s superior thermoelectrochemical performance under substantial deformations and repeated self-healing cycles.The resulting hydrogel-based TEC yields a maximum power output of 1032.1 nW under theΔT of 20 K when being stretched to 500%for 1000 cycles,corresponding to 80%of its initial state;meanwhile,it sustains 1179.1 nW under theΔT of 20 K even after 60 cuthealing cycles,approximately 92%of its initial state.The as-assembled TEC array exhibits device-level self-healing capability and high adaptability to human body.It is readily applied for touch-based encrypted communication where distinct voltage signals can be converted into alphabet letters;it is also employed as a self-powered sensor to in-situ monitor a variety of body motions for complex human actions.The swift assembly approach,combined with the versatile functionality of the TEC device,paves the way for future advancements in wearable electronics targeting at fitness monitoring and human–machine interfaces.展开更多
Two-dimensional(2D) semiconductors have captured broad interest as light emitters, due to their unique excitonic effects. These layer-blocks can be integrated through van der Waals assembly, i.e., fabricating homo-or ...Two-dimensional(2D) semiconductors have captured broad interest as light emitters, due to their unique excitonic effects. These layer-blocks can be integrated through van der Waals assembly, i.e., fabricating homo-or heterojunctions, which show novel emission properties caused by interface engineering. In this review, we will first give an overview of the basic strategies that have been employed in interface engineering, including changing components, adjusting interlayer gap, and tuning twist angle. By modifying the interfacial factors, novel emission properties of emerging excitons are unveiled and discussed. Generally, well-tailored interfacial energy transfer and charge transfer within a 2D heterostructure cause static modulation of the brightness of intralayer excitons. As a special case, dynamically correlated dual-color emission in weakly-coupled bilayers will be introduced, which originates from intermittent interlayer charge transfer. For homobilayers and type Ⅱ heterobilayers, interlayer excitons with electrons and holes residing in neighboring layers are another important topic in this review. Moreover, the overlap of two crystal lattices forms moiré patterns with a relatively large period, taking effect on intralayer and interlayer excitons. Particularly, theoretical and experimental progresses on spatially modulated moiré excitons with ultra-sharp linewidth and quantum emission properties will be highlighted. Moiré quantum emitter provides uniform and integratable arrays of single photon emitters that are previously inaccessible, which is essential in quantum many-body simulation and quantum information processing. Benefiting from the optically addressable spin and valley indices, 2D heterostructures have become an indispensable platform for investigating exciton physics, designing and integrating novel concept emitters.展开更多
Powder hot isostatic pressing(HIP) is an effective method to achieve near-net-shape manufacturing of high-quality complex thinwalled titanium alloy parts, and it has received extensive attention in recent years. Howev...Powder hot isostatic pressing(HIP) is an effective method to achieve near-net-shape manufacturing of high-quality complex thinwalled titanium alloy parts, and it has received extensive attention in recent years. However, there are few reports about the microstructure characteristics on the strengthening and toughening mechanisms of powder hot isostatic pressed(HIPed) titanium alloys. Therefore, TA15powder was prepared into alloy by HIP approach, which was used to explore the microstructure characteristics at different HIP temperatures and the corresponding tensile properties and fracture toughness. Results show that the fabricated alloy has a “basket-like structure” when the HIP temperature is below 950℃, consisting of lath clusters and surrounding small equiaxed grains belts. When the HIP temperature is higher than 950℃, the microstructure gradually transforms into the Widmanstatten structure, accompanied by a significant increase in grain size. The tensile strength and elongation are reduced from 948 MPa and 17.3% for the 910℃ specimen to 861 MPa and 10% for the 970℃ specimen.The corresponding tensile fracture mode changes from transcrystalline plastic fracture to mixed fracture including intercrystalline cleavage.The fracture toughness of the specimens increases from 82.64 MPa·m^(1/2)for the 910℃ specimen to 140.18 MPa·m^(1/2)for the 970℃ specimen.Specimens below 950℃ tend to form holes due to the prior particle boundaries(PPBs), which is not conducive to toughening. Specimens above 950℃ have high fracture toughness due to the crack deflection, crack branching, and shear plastic deformation of the Widmanstatten structure. This study provides a valid reference for the development of powder HIPed titanium alloy.展开更多
Decarbonization is a critical issue for peaking CO_(2) emissions of energy-intensive industries,such as the iron and steel industry.The decarbonization options of China’s ironmaking and steelmaking sector were discus...Decarbonization is a critical issue for peaking CO_(2) emissions of energy-intensive industries,such as the iron and steel industry.The decarbonization options of China’s ironmaking and steelmaking sector were discussed based on a systematic three-dimensional low-carbon analysis from the aspects of resource utilization(Y),energy utilization(Q),and energy cleanliness which is evaluated by a process general emission factor(PGEF)on all the related processes,including the current blast furnace(BF)-basic oxygen furnace(BOF)integrated process and the specific sub-processes,as well as the electric arc furnace(EAF)process,typical direct reduction(DR)process,and smelting reduction(SR)process.The study indicates that the three-dimensional aspects,particularly the energy structure,should be comprehensively considered to quantitatively evaluate the decarbonization road map based on novel technologies or processes.Promoting scrap utilization(improvement of Y)and the substitution of carbon-based energy(improvement of PGEF)in particular is critical.In terms of process scale,promoting the development of the scrap-based EAF or DR-EAF process is highly encouraged because of their lower PGEF.The three-dimensional method is expected to extend to other processes or industries,such as the cement production and thermal electricity generation industries.展开更多
Flowering time(FT) is a key maize domestication trait, variation in which allows maize to grow in a wide range of latitudes. Although previous studies have investigated the genetic control of FT-related traits per se,...Flowering time(FT) is a key maize domestication trait, variation in which allows maize to grow in a wide range of latitudes. Although previous studies have investigated the genetic control of FT-related traits per se, few studies of FT hybrid performance have been published. We characterized the genomic architecture associated with hybrid performance for FT in a hybrid panel by testcrossing Chang 7–2 with 328Ye478 × Qi319 recombinant inbred lines(RILs). We identified 11 quantitative trait loci(QTL) for hybrid performance in FT-related traits, including a major QTL qFH10 that controls hybrid performance and heterosis in a summer maize-growing region. However, this locus acts in regulating FT traits per se only in a spring maize-growing region. We validated ZmCCT10 as a candidate gene for qFH10 and found that differences between hybrids and their parental lines in DNA methylation in the differentially methylated region(DMR, –700 to –1520) of the ZmCCT10 promoter affected gene expression pattern and thereby FT in the summer maize-growing region.展开更多
Objective C-reactive protein(CRP)/albumin ratio(CAR)is a new inflammation-based index for predicting the prognosis of various diseases.The CAR determined on admission may help to predict the prognostic value of multip...Objective C-reactive protein(CRP)/albumin ratio(CAR)is a new inflammation-based index for predicting the prognosis of various diseases.The CAR determined on admission may help to predict the prognostic value of multiple trauma patients.Methods A total of 264 adult patients with severe multiple trauma were included for the present retrospective study,together with the collection of relevant clinical and laboratory data.CAR,CRP,albumin,shock index and ISS were incorporated into the prognostic model,and the receiver operating characteristic(ROC)curve was drawn.Then,the shock index for patients with different levels of CAR was analyzed.Finally,univariate and multivariate logistic regression analyses were performed to identify the independent risk factors for the 28-day mortality of multiple trauma patients.Results A total of 36 patients had poor survival outcomes,and the mortality rate reached 13.6%.Furthermore,after analyzing the shock index for patients with different levels of CAR,it was revealed that the shock index was significantly higher when CAR was≥4,when compared to CAR<2 and 2≤CAR<4,in multiple trauma patients.The multivariate logistic analysis helped to identify the independent association between the variables CAR(P=0.029)and shock index(P=0.019),and the 28-day mortality of multiple trauma patients.Conclusion CAR is higher in patients with severe multiple trauma.Furthermore,CAR serves as a risk factor for independently predicting the 28-day mortality of multiple trauma patients.The shock index was significantly higher when CAR was≥4 in multiple trauma patients.展开更多
基金supported by the National Natural Science Foundation of China(No.81973762).
文摘Objective:To explore the efficacy and potential mechanisms of the ethanol extract of Abelmoschus manihot(L.)Medic in contrast-induced nephropathy(CIN).Methods:CIN rat models and human renal proximal tubular cells(HK-2)with iopromide-induced injury were employed to mimic CIN conditions.The effect of Abelmoschus manihot extract on the rat models and HK-2 cells was evaluated.In rat models,kidney function,histology,oxidative stress and apoptosis were determined.In HK-2 cells,cell viability,apoptosis,mitochondrial membrane potential,and endoplasmic reticulum stress were assessed.Results:Abelmoschus manihot extract significantly improved structural and functional impairments in the kidneys of CIN rats.Additionally,the extract effectively mitigated the decline in cellular viability and reduced iopromide-induced oxidative stress and lipid peroxidation.Mechanistic investigations revealed that Abelmoschus manihot extract prominently attenuated acute endoplasmic reticulum stress-mediated apoptosis by downregulating GRP78 and CHOP protein levels.Conclusions:Abelmoschus manihot extract can be used as a promising therapeutic and preventive agent in the treatment of CIN.
基金supported by the National High Level Hospital Clinical Research Fund (2022-PUMCH-B-109)CAMS Innovation Fund for Medical Sciences (CIFMS)(2021-1-I2M-020)。
文摘BACKGROUND:Sepsis-associated encephalopathy(SAE) is a critical disease caused by sepsis.In addition to high mortality,SAE can also adversely aff ect life quality and lead to significant socioeconomic costs.This review aims to explore the development of evaluation animal models of SAE,giving insight into the direction of future research in terms of its pathophysiology and therapy.METHODS:We performed a literature search from January 1,2000,to December 31,2022,in MEDLINE,PubMed,EMBASE,and Web of Science using related keywords.Two independent researchers screened all the accessible articles based on the inclusion and exclusion criteria and collected the relevant data of the studies.RESULTS:The animal models for sepsis are commonly induced through cecal ligation and puncture(CLP) or lipopolysaccharide(LPS) injection.SAE can be evaluated using nervous reflex scores and sepsis evaluation during the acute phase,or through Morris water maze(MWM),openfield test,fear condition(FC) test,inhibitory avoidance,and other tests during the late phase.CONCLUSION:CLP and LPS injection are the most common methods for establishing SAE animal models.Nervous reflexs cores,MWM,FC test,and inhibitory avoidance are widely used in SAE model analysis.Future research should focus on establishing a standardized system for SAE development and analysis.
基金Supported by Grants from the China Medical Board of New York,No.11-045National Key Technology Research and Development Program of China,No.BAI06B01
文摘AIM:To analyze the expression profiles of long noncoding RNAs(lncRNAs)in hepatocellular carcinoma.METHODS:Hepatocellular carcinoma(HCC)tissues and matched adjacent non-tumor(NT)liver tissues were collected from 29 patients with HCC,immediately after liver resection,between March 2011 and July2013.The diagnosis of HCC was made based on histological examination.Differentially expressed lncRNAs between HCC and NT tissues were revealed through microarray-based lncRNAs expression profiling.Further,quantification of selected lncRNAs was performed using quantitative real-time reverse transcription polymerase chain reaction(qRT-PCR).RESULTS:Six hundred and fifty-nine lncRNAs were differentially expressed between HCC and NT tissues,of which five[TCONS_00018278,AK093543,D16366,ENST00000501583,NR_002819(MALAT1)]were selected for validation.Four of them were significantly downregulated in HCC tissues compared with NT tissues(P=0.012,0.045,0.000 and 0.000,respectively),and the expression level of MALAT1 showed no significant difference(P=0.114).CONCLUSION:This study identified a set of lncRNAs differentially expressed in HCC tissues and provided useful information for exploring potential therapeutic targets and diagnostic biomarkers of this cancer.
基金supported by grants from the National Key Technology Research and Development Program of China 2012(BAI06B01)the National Natural Science Foundation of China(81201566)+1 种基金the Specialized Research Fund for the Doctoral Program of Higher Education(20121106110002)Wu Jieping Medical Foundation(LDWMF-SY-2011B002)
文摘BACKGROUND: Golgi protein 73(GP73) is a promising biomarker of hepatocellular carcinoma(HCC). It decreases after surgical resection, and resumes upon recurrence, indicating a potential indicator for the effectiveness of the treatment. But changes of GP73 after transcatheter arterial chemoembolization(TACE) have not been reported so far. This study was to investigate the dynamic changes of GP73 in HCC patients after TACE treatment, and the possible underlying mechanisms in the cell cultures.METHODS: Blood samples were collected from 72 HCC patients, before TACE, at day 1 and day 30 after TACE. GP73 levels were measured by Western blotting. The dynamic changes of GP73 were analyzed and compared with image changes and clinical data. The effects of chemotherapeutic agents(5-FU and pirarubicin) on GP73 expression were tested in three HCC cell lines(Hep G2, HCCLM3 and MHCC97H).RESULTS: The GP73 level was significantly elevated at day 1and day 30 after TACE in HCC patients compared with that before the procedure(P<0.05). There was no statistical difference between the two time points after TACE, nor correlationbetween GP73 levels and clinicopathological features, tumor metastasis, and patient survival. Pirarubicin, not 5-FU, significantly increased GP73 expression in three cell lines. CONCLUSIONS: Unlike surgical resection which decreases the GP73 level, TACE significantly increased GP73 expression in patients with HCC. No correlations were observed among GP73 levels, tumor characteristics and prognosis of patients with HCC.
基金Supported by CAMS Innovation Fund for Medical Sciences,No.2016-I2M-1-001Tsinghua University-Peking Union Medical College Hospital Cooperation Project,No.PTQH201904552。
文摘BACKGROUND Gallbladder carcinoma(GBC)carries a poor prognosis and requires a prediction method.Gamma-glutamyl transferase–to–platelet ratio(GPR)is a recently reported cancer prognostic factor.Although the mechanism for the relationship between GPR and poor cancer prognosis remains unclear,studies have demonstrated the clinical effect of both gamma-glutamyl transferase and platelet count on GBC and related gallbladder diseases.AIM To assess the prognostic value of GPR and to design a prognostic nomogram for GBC.METHODS The analysis involved 130 GBC patients who underwent surgery at Peking Union Medical College Hospital from December 2003 to April 2017.The patients were stratified into a high-or low-GPR group.The predictive ability of GPR was evaluated by Kaplan–Meier analysis and a Cox regression model.We developed a nomogram based on GPR,which we verified using calibration curves.The nomogram and other prognosis prediction models were compared using timedependent receiver operating characteristic curves and the concordance index.RESULTS Patients in the high-GPR group had a higher risk of jaundice,were older,and had higher carbohydrate antigen 19-9 levels and worse postoperative outcomes.Univariate analysis revealed that GPR,age,body mass index,tumor–node–metastasis(TNM)stage,jaundice,cancer cell differentiation degree,and carcinoembryonic antigen and carbohydrate antigen 19-9 levels were related to overall survival(OS).Multivariate analysis confirmed that GPR,body mass index,age,and TNM stage were independent predictors of poor OS.Calibration curves were highly consistent with actual observations.Comparisons of timedependent receiver operating characteristic curves and the concordance index showed advantages for the nomogram over TNM staging.CONCLUSION GPR is an independent predictor of GBC prognosis,and nomogram-integrated GPR is a promising predictive model for OS in GBC.
基金the National Basic Research Program of China(No.2011CB606306)the National High Technology Research and Development Program of China(No.2009AA033201)+3 种基金the National Natural Science Foundation of China(No.50974017)the Fundamental Research Funds for the Central Universities(No.FRF-TP-11-004A)the Program for New Century Excellent Talentsin Universities of China(No.NCET-10-0226)Fok Ying Tung Education Foundation for Young College Teachers(No.122016)
文摘Nb-based powder was fabricated via mechanical grinding. The influence of stearic acid on the grinding process was studied. The structural evolution and morphological evolution of the milled powder were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS) analysis. It is indicated that an appropriate amount of stearic acid accelerates the particle refinement process and favors the production of superfine Nb-based particles with good dispersivity and high activity. However, an inappropriate amount of stearic acid has an adverse effect on the refinement process.
基金Supported by National Natural Science Foundation of China,No.30901453 and No.81201566National Key Technology Research and Development Program of China,No.BAI06B01Youth Grant of Peking Union Medical College Hospital
文摘AIM To investigate the evaluation of neogalactosylalbumin(NGA) for liver function assessment based on positron emission tomography technology.METHODS Female Kunming mice were assigned randomly to two groups: fibrosis group and normal control group. A murine hepatic fibrosis model was generated by intraperitoneal injection of 10% carbon tetrachloride(CCl_4) at 0.4 m L every 48 h for 42 d. ^(18)F-labeled NGA([^(18)F]FNGA) was synthesized and administered at a dosage of 3.7 MBq/mouse to both fibrosis mice and normal control mice. Distribution of [^(18)F]FNGA amongst organs was examined, and dynamic scanning was performed. Parameters were set up to compare the uptake of tracers by fibrotic liver and healthy liver. Serologic tests for liver function were also performed.RESULTS The liver function of the fibrosis model mice was significantly impaired by the use of CCl_4. In the fibrosis model mice, hepatic fibrosis was verified by naked eye assessment and pathological analysis. [^(18)F]FNGA was found to predominantly accumulate in liver and kidneys in both control group(n = 21) and fibrosis group(n = 23). The liver uptake ability(LUA), peak time(T_p), and uptake rate(LUR) of [^(18)F]FNGA between healthy liver(n = 8) and fibrosis liver(n = 10) were significantly different(P < 0.05, < 0.01, and < 0.05, respectively). LUA was significantly correlated with total serum protein level(TP)(P < 0.05). T_p was significantly correlated with both TP and glucose(Glu) concentration(P < 0.05 both), and LUR was significantly correlated with both total bile acid and Glu concentration(P < 0.01 and < 0.05, respectively).CONCLUSION[^(18)F]FNGA mainly accumulated in liver and remained for sufficient time. Functionally-impaired liver showed a significant different uptake pattern of [^(18)F]FNGA compared to the controls.
文摘Objective: To study the effects of astragalus polysaccharide on cell injury and mitochondrial pathway apoptosis in the hypoxia reoxygenation of myocardial cells. Methods: H9c2 myocardial cell lines were cultured and divided into negative control group (NC group), hypoxia reoxygenation group (H/R group) and astragalus polysaccharide group (APS), H/R group underwent hypoxia reoxygenation treatment, and APS group underwent both hypoxia reoxygenation treatment and astragalus polysaccharides intervention. The cell viability was measured 8 h, 16 h and 24 h after reoxygenation;the expression of mitochondrial apoptosis genes, apoptosis pathway genes as well as the contents of ROS metabolism indexes were determined 24 h after reoxygenation. Results: 8 h, 16 h and 24 h after reoxygenation, the cell viability of H/R group were lower than those of NC group, and the cell viability of APS group were higher than those of H/R group;24 h after reoxygenation, BIM, BAX, Caspase-9, Caspase-3, p-PI3K, p-AKT and p-FoxO3a protein expression as well as ROS, HSP70 and p-p38MAPK contents in H/R group were significantly higher than those in NC group whereas BCL2 protein expression and SOD content were significantly lower than those in NC group;BIM, BAX, Caspase-9, Caspase-3, p-PI3K, p-AKT and p-FoxO3a protein expression as well as ROS, HSP70 and p-p38MAPK contents in APS group were significantly lower than those in H/R group whereas BCL2 protein expression and SOD content were significantly higher than those in H/R group. Conclusion: Astragalus polysaccharide can reduce the cell damage and inhibit the mitochondrial pathway apoptosis in the hypoxia reoxygenation process of myocardial cells.
基金supported by the National Natural Science Foundation of China(Nos.51922004,51874037)State Key Lab of Advanced Metals and Materials,University of Science and Technology Beijing,China(Nos.2019Z-14,2020Z-04,2021Z-03)+7 种基金Fundamental Research Funds for the Central Universities,China(Nos.FRF-TP-19005C1Z,06500236)Interdisciplinary Research Project for Young Teachers of USTB,China(Fundamental Research Funds for the Central Universities,FRF-IDRY-20-023)Postdoctor Research Foundation of Shunde Graduate School of University of Science and Technology Beijing,China(No.2022BH001)the China Postdoctoral Science Foundation(No.2021M700377)the Beijing Natural Science Foundation,China(No.2212035)the support from the European Commission via the H2020 MSCA RISE BAMOS program(No.734156)Innovate UK via Newton Fund(No.102872)Engineering and Physical Science Research Council(EPSRC)via DTP case programme(No.EP/T517793/1)。
基金support by the National Natural Science Foundation of China(52103089)Guangdong Basic and Applied Basic Research Foundation(2023A1515012120)+2 种基金Shenzhen Science and Technology Program(JCYJ20220531100815035,RCBS20221008093126069)the Opening Project of State Key Laboratory of Polymer Materials Engineering(Grant No.sklpme2022-4-08)the Instrumental Analysis Center of Shenzhen University(Lihu Campus)for their assistance with SEM characterization.
文摘The evolution of wearable technology has prompted the need for adaptive,self-healable,and energy-autonomous energy devices.This study innovatively addresses this challenge by introducing an MXene-boosted hydrogel electrolyte,which expedites the assembly process of flexible thermocell(TEC)arrays and thus circumvents the complicated fabrication of typical wearable electronics.Our findings underscore the hydrogel electrolyte’s superior thermoelectrochemical performance under substantial deformations and repeated self-healing cycles.The resulting hydrogel-based TEC yields a maximum power output of 1032.1 nW under theΔT of 20 K when being stretched to 500%for 1000 cycles,corresponding to 80%of its initial state;meanwhile,it sustains 1179.1 nW under theΔT of 20 K even after 60 cuthealing cycles,approximately 92%of its initial state.The as-assembled TEC array exhibits device-level self-healing capability and high adaptability to human body.It is readily applied for touch-based encrypted communication where distinct voltage signals can be converted into alphabet letters;it is also employed as a self-powered sensor to in-situ monitor a variety of body motions for complex human actions.The swift assembly approach,combined with the versatile functionality of the TEC device,paves the way for future advancements in wearable electronics targeting at fitness monitoring and human–machine interfaces.
基金supported by the Natural Science Foundation of China(22203042,21873048 and 22173044)。
文摘Two-dimensional(2D) semiconductors have captured broad interest as light emitters, due to their unique excitonic effects. These layer-blocks can be integrated through van der Waals assembly, i.e., fabricating homo-or heterojunctions, which show novel emission properties caused by interface engineering. In this review, we will first give an overview of the basic strategies that have been employed in interface engineering, including changing components, adjusting interlayer gap, and tuning twist angle. By modifying the interfacial factors, novel emission properties of emerging excitons are unveiled and discussed. Generally, well-tailored interfacial energy transfer and charge transfer within a 2D heterostructure cause static modulation of the brightness of intralayer excitons. As a special case, dynamically correlated dual-color emission in weakly-coupled bilayers will be introduced, which originates from intermittent interlayer charge transfer. For homobilayers and type Ⅱ heterobilayers, interlayer excitons with electrons and holes residing in neighboring layers are another important topic in this review. Moreover, the overlap of two crystal lattices forms moiré patterns with a relatively large period, taking effect on intralayer and interlayer excitons. Particularly, theoretical and experimental progresses on spatially modulated moiré excitons with ultra-sharp linewidth and quantum emission properties will be highlighted. Moiré quantum emitter provides uniform and integratable arrays of single photon emitters that are previously inaccessible, which is essential in quantum many-body simulation and quantum information processing. Benefiting from the optically addressable spin and valley indices, 2D heterostructures have become an indispensable platform for investigating exciton physics, designing and integrating novel concept emitters.
基金financially supported by the National Natural Science Foundation of China (Nos. 51874037 and 51922004)the Beijing Natural Science Foundation (No. 2212035)+1 种基金the Fundamental Research Funds for the Central Universities (No. FRF-TP-19005C1Z)the National Defense Basic Research Project (No. JCKY2017213004)。
文摘Powder hot isostatic pressing(HIP) is an effective method to achieve near-net-shape manufacturing of high-quality complex thinwalled titanium alloy parts, and it has received extensive attention in recent years. However, there are few reports about the microstructure characteristics on the strengthening and toughening mechanisms of powder hot isostatic pressed(HIPed) titanium alloys. Therefore, TA15powder was prepared into alloy by HIP approach, which was used to explore the microstructure characteristics at different HIP temperatures and the corresponding tensile properties and fracture toughness. Results show that the fabricated alloy has a “basket-like structure” when the HIP temperature is below 950℃, consisting of lath clusters and surrounding small equiaxed grains belts. When the HIP temperature is higher than 950℃, the microstructure gradually transforms into the Widmanstatten structure, accompanied by a significant increase in grain size. The tensile strength and elongation are reduced from 948 MPa and 17.3% for the 910℃ specimen to 861 MPa and 10% for the 970℃ specimen.The corresponding tensile fracture mode changes from transcrystalline plastic fracture to mixed fracture including intercrystalline cleavage.The fracture toughness of the specimens increases from 82.64 MPa·m^(1/2)for the 910℃ specimen to 140.18 MPa·m^(1/2)for the 970℃ specimen.Specimens below 950℃ tend to form holes due to the prior particle boundaries(PPBs), which is not conducive to toughening. Specimens above 950℃ have high fracture toughness due to the crack deflection, crack branching, and shear plastic deformation of the Widmanstatten structure. This study provides a valid reference for the development of powder HIPed titanium alloy.
基金supported by the State Key Laboratory of Advanced Metallurgy,China(Project Code:41603006).
文摘Decarbonization is a critical issue for peaking CO_(2) emissions of energy-intensive industries,such as the iron and steel industry.The decarbonization options of China’s ironmaking and steelmaking sector were discussed based on a systematic three-dimensional low-carbon analysis from the aspects of resource utilization(Y),energy utilization(Q),and energy cleanliness which is evaluated by a process general emission factor(PGEF)on all the related processes,including the current blast furnace(BF)-basic oxygen furnace(BOF)integrated process and the specific sub-processes,as well as the electric arc furnace(EAF)process,typical direct reduction(DR)process,and smelting reduction(SR)process.The study indicates that the three-dimensional aspects,particularly the energy structure,should be comprehensively considered to quantitatively evaluate the decarbonization road map based on novel technologies or processes.Promoting scrap utilization(improvement of Y)and the substitution of carbon-based energy(improvement of PGEF)in particular is critical.In terms of process scale,promoting the development of the scrap-based EAF or DR-EAF process is highly encouraged because of their lower PGEF.The three-dimensional method is expected to extend to other processes or industries,such as the cement production and thermal electricity generation industries.
基金jointly funded by the National Natural Science Foundation of China (31971963)Agricultural Science and Technology Innovation Program of CAAS。
文摘Flowering time(FT) is a key maize domestication trait, variation in which allows maize to grow in a wide range of latitudes. Although previous studies have investigated the genetic control of FT-related traits per se, few studies of FT hybrid performance have been published. We characterized the genomic architecture associated with hybrid performance for FT in a hybrid panel by testcrossing Chang 7–2 with 328Ye478 × Qi319 recombinant inbred lines(RILs). We identified 11 quantitative trait loci(QTL) for hybrid performance in FT-related traits, including a major QTL qFH10 that controls hybrid performance and heterosis in a summer maize-growing region. However, this locus acts in regulating FT traits per se only in a spring maize-growing region. We validated ZmCCT10 as a candidate gene for qFH10 and found that differences between hybrids and their parental lines in DNA methylation in the differentially methylated region(DMR, –700 to –1520) of the ZmCCT10 promoter affected gene expression pattern and thereby FT in the summer maize-growing region.
基金supported by Jiangsu Provincial Medical Innovation Center of Jiangsu Province Capability Improvement Project through Science,Technology and Education(No.CXZX202231)the Special Research Topic on Innovation of Hospital Management,Jiangsu Provincial Hospital Association(No.JSYGY-3-2021-JZ71).
文摘Objective C-reactive protein(CRP)/albumin ratio(CAR)is a new inflammation-based index for predicting the prognosis of various diseases.The CAR determined on admission may help to predict the prognostic value of multiple trauma patients.Methods A total of 264 adult patients with severe multiple trauma were included for the present retrospective study,together with the collection of relevant clinical and laboratory data.CAR,CRP,albumin,shock index and ISS were incorporated into the prognostic model,and the receiver operating characteristic(ROC)curve was drawn.Then,the shock index for patients with different levels of CAR was analyzed.Finally,univariate and multivariate logistic regression analyses were performed to identify the independent risk factors for the 28-day mortality of multiple trauma patients.Results A total of 36 patients had poor survival outcomes,and the mortality rate reached 13.6%.Furthermore,after analyzing the shock index for patients with different levels of CAR,it was revealed that the shock index was significantly higher when CAR was≥4,when compared to CAR<2 and 2≤CAR<4,in multiple trauma patients.The multivariate logistic analysis helped to identify the independent association between the variables CAR(P=0.029)and shock index(P=0.019),and the 28-day mortality of multiple trauma patients.Conclusion CAR is higher in patients with severe multiple trauma.Furthermore,CAR serves as a risk factor for independently predicting the 28-day mortality of multiple trauma patients.The shock index was significantly higher when CAR was≥4 in multiple trauma patients.
