Over the past decade,the swift advancement of metabolomics can be credited to significant progress in technologies such as mass spectrometry,nuclear magnetic resonance,and multivariate statistics.Currently,metabolomic...Over the past decade,the swift advancement of metabolomics can be credited to significant progress in technologies such as mass spectrometry,nuclear magnetic resonance,and multivariate statistics.Currently,metabolomics garners widespread application across diverse fields including drug research and development,early disease detection,toxicology,food and nutrition science,biology,prescription,and chinmedomics,among others.Metabolomics serves as an effective characterization technique,offering insights into physiological process alterations in vivo.These changes may result from various exogenous factors like environmental conditions,stress,medications,as well as endogenous elements including genetic and protein-based influences.The potential scientific outcomes gleaned from these insights have catalyzed the formulation of innovative methods,poised to further broaden the scope of this domain.Today,metabolomics has evolved into a valuable and widely accepted instrument in the life sciences.However,comprehensive reviews focusing on the sample preparation and analytical methodologies employed in metabolomics within the life sciences are surprisingly scant.This review aims to fill that gap,providing an overview of current trends and recent advancements in metabolomics.Particular emphasis is placed on sample preparation,sophisticated analytical techniques,and their applications in life science research.展开更多
Objective: To analyze the potential mechanism of Xinyi San in treating rhinitis through network pharmacology. Methods: In the database of Traditional Chinese Medicine Systems Pharmacology (TCMSP), chemical composition...Objective: To analyze the potential mechanism of Xinyi San in treating rhinitis through network pharmacology. Methods: In the database of Traditional Chinese Medicine Systems Pharmacology (TCMSP), chemical composition and potential targets of Xinyi San were got, and the target genes of rhinitis of Xinyi San were extracted from GeneCards databases. Then we constructed protein-protein interactions (PPI) network of target genes, and then analyzed the Key genes in GO analysis and KEGG analysis. Results: We got 97 components, 53 potential therapeutic targets, 1009 GO items and 92 pathways in our study. The main pathways included Lipid and atherosclerosis, Chemical carcinogenesis-receptor activation, PI3K-Akt signaling pathway, Human cytomegalovirus infection, Prostate cancer, etc. Conclusion: Xinyi San plays a role in treating rhinitis through multiple components, multiple targets and multiple pathways.展开更多
In recent years,microbial metabolomics,a new field that has attracted wide attention,provides a map of metabolic pathways and clarifies the interaction mechanism between microorganisms and hosts.Many microorganisms ar...In recent years,microbial metabolomics,a new field that has attracted wide attention,provides a map of metabolic pathways and clarifies the interaction mechanism between microorganisms and hosts.Many microorganisms are found in the human intestine,oral cavity,vagina,etc.Probiotics could maintain the good health of the host,while pathogens and an imbalance of bacterial flora lead to a series of diseases of the body and mind.Metabolomics is a science for qualitative and quantitative analysis of all metabolites in an organism or biological system,which could provide key information to understand the related metabolic pathways and associated changes.This approach analyzes the final products of cellular regulatory processes,the level of which can be regarded as the ultimate response of the biological system to genetic or environmental changes.Microbial metabolomics has been widely used in different research fields,such as microbial phenotypic classification,mutant screening,metabolic pathways,microbial metabolic engineering,fermentation engineering monitoring and optimization,microbial environmental pollution,and so on.However,there are only a few reviews on microbial metabolomics of probiotics and pathogens.This review summarizes the main methodologies,including sample preparation,identification of metabolites,data processing,and analysis.Recent applications in microbial metabolomics of probiotics and pathogens are also described.This paper first summarized the research progress and application of microbial metabolomics from two aspects:probiotics and pathogenic bacteria.Probiotics and pathogenic bacteria do not exist independently most of the time;hence,these were reviewed in the research field of coexistence of probiotics and pathogenic bacteria,which was subdivided into important microbial research fields closely related to human health,including the human gut,oral cavity,food,and nutrition-related microorganisms.Then,the main problems and trends associated with microbial metabolomics are discussed.展开更多
This study established a novel method for the simultaneous detection of two-component gases.Radio frequency(RF)white noise disturbance laser current and wavelength modulation were simultaneously used to improve the of...This study established a novel method for the simultaneous detection of two-component gases.Radio frequency(RF)white noise disturbance laser current and wavelength modulation were simultaneously used to improve the off-axis integrated cavity output spectroscopy technique,and a high-precision dual modulation OA-ICOS(RF-WM-OA-ICOS)system was established.The two laser beams were coupled into one laser beam that was applied incident to the cavity of RF-WM-OA-ICOS system.The second harmonic signals of CH_(4)and CO_(2)gas simultaneously appeared in the rising or falling edge of a triangular wave.This method was used to measure CH_(4)and CO_(2)with different concentrations.The results indicated that the proposed system has high stability and can accurately and simultaneously measure the concentrations of CH_(4)and CO_(2),with an optimal integration time of 220 s.The minimum detection limit was 10 ppb for CH_(4)and 1.5 ppm for CO_(2).The corresponding noise equivalent absorption sensitivity values were calculated as 2.67×10^(-13)cm^(-1)·Hz^(-1/2)and 5.18×10^(-11)cm^(-1)·Hz^(-1/2),respectively.The proposed dual-component gas simultaneous detection method can also be used for high-precision simultaneous detection of other gases.Therefore,this study may serve as a reference for developing portable multicomponent gas analyzers.展开更多
[Objectives]To investigate the spasticity of rat tail and the expression of dopamine receptor-1(DRD1)mRNA in the spinal cord after spinal cord injury(SCI)induced tail spasticity in rats.[Methods]Adult male Wistar rats...[Objectives]To investigate the spasticity of rat tail and the expression of dopamine receptor-1(DRD1)mRNA in the spinal cord after spinal cord injury(SCI)induced tail spasticity in rats.[Methods]Adult male Wistar rats were randomly divided into Sham group and SCI group.The second sacral spinal cord(S2)segment of SCI rats was completely transected.60 d after operation,the rat tail spasticity was scored,and then the spinal cord tissues below the level of S2 spinal cord transection were taken.The expression of DRD1 mRNA in the sacrococcygeal spinal cord was detected by qPCR.In addition,3 normal rats were used for DAR/neuronal nuclei(NeuN)and DRD1/choline acetyltransferase(ChAT)immunofluorescence staining to study the distribution of DRD1 in spinal cord and the properties of DRD1 positive cells.[Results]60 d after operation in SCI group,the tail spasticity of rats developed fully,and the symptoms of spasticity were typical.qPCR results showed that the expression of DRD1 mRNA in SCI group was significantly lower than that in Sham group(P<0.05).DRD1 was widely distributed in the dorsal horn,intermediate zone and ventral horn at the sacrococcygeal end of the rat spinal cord.[Conclusions]The decrease of DRD1 mRNA expression after SCI may be related to the occurrence and development of spasticity.展开更多
采用硅烷偶联剂Z6020对六方氮化硼(h-BN)进行改性,通过原位聚合法制备聚酰亚胺/改性氮化硼(PI/f-BN)复合薄膜。利用红外光谱仪、自制调频耐电晕装置和宽频介电谱测试仪对复合薄膜进行测试,采用光学显微镜对薄膜耐电晕老化后的击穿孔形...采用硅烷偶联剂Z6020对六方氮化硼(h-BN)进行改性,通过原位聚合法制备聚酰亚胺/改性氮化硼(PI/f-BN)复合薄膜。利用红外光谱仪、自制调频耐电晕装置和宽频介电谱测试仪对复合薄膜进行测试,采用光学显微镜对薄膜耐电晕老化后的击穿孔形貌进行表征。结果表明:在棒板空气间隙为1 mm,脉冲电场强度为50 k V/mm,占空比为50%,频率为20 k Hz的测试条件下,随着f-BN含量的提高,薄膜的耐电晕寿命呈先增加后缩短的趋势。当f-BN含量为15%时,复合薄膜的介电常数最大,耐电晕寿命最长。展开更多
In order to enhance modeling efficiency and accuracy,we utilized 3D laser point cloud data for indoor space modeling.Point cloud data was obtained with a 3D laser scanner and optimized with Autodesk Recap and Revit so...In order to enhance modeling efficiency and accuracy,we utilized 3D laser point cloud data for indoor space modeling.Point cloud data was obtained with a 3D laser scanner and optimized with Autodesk Recap and Revit software to extract geometric information about the indoor environment.Furthermore,we proposed a method for constructing indoor elements based on parametric components.The research outcomes of this paper will offer new methods and tools for indoor space modeling and design.The approach of indoor space modeling based on 3D laser point cloud data and parametric component construction can enhance modeling efficiency and accuracy,providing architects,interior designers,and decorators with a better working platform and design reference.展开更多
Requirements for the quality of housing have been increasing in recent years.Many existing buildings can no longer meet the needs of residents due to functional,layout,and other issues,and need to be renovated.However...Requirements for the quality of housing have been increasing in recent years.Many existing buildings can no longer meet the needs of residents due to functional,layout,and other issues,and need to be renovated.However,traditional renovation methods have problems such as high pollution and high consumption.With the rapid development of prefabricated construction and green environmental protection concepts,the problems of traditional interior renovation can be resolved using prefabricated interior decoration.As a new and advanced method of renovation,prefabricated renovation reduces construction segments,workload,pollution,and waste,and it promotes the upgrading of the traditional renovation industry.展开更多
基金supported by the Science Foundation of Heilongjiang Administration of Traditional Chinese Medicine(No.2018-21).
文摘Over the past decade,the swift advancement of metabolomics can be credited to significant progress in technologies such as mass spectrometry,nuclear magnetic resonance,and multivariate statistics.Currently,metabolomics garners widespread application across diverse fields including drug research and development,early disease detection,toxicology,food and nutrition science,biology,prescription,and chinmedomics,among others.Metabolomics serves as an effective characterization technique,offering insights into physiological process alterations in vivo.These changes may result from various exogenous factors like environmental conditions,stress,medications,as well as endogenous elements including genetic and protein-based influences.The potential scientific outcomes gleaned from these insights have catalyzed the formulation of innovative methods,poised to further broaden the scope of this domain.Today,metabolomics has evolved into a valuable and widely accepted instrument in the life sciences.However,comprehensive reviews focusing on the sample preparation and analytical methodologies employed in metabolomics within the life sciences are surprisingly scant.This review aims to fill that gap,providing an overview of current trends and recent advancements in metabolomics.Particular emphasis is placed on sample preparation,sophisticated analytical techniques,and their applications in life science research.
文摘Objective: To analyze the potential mechanism of Xinyi San in treating rhinitis through network pharmacology. Methods: In the database of Traditional Chinese Medicine Systems Pharmacology (TCMSP), chemical composition and potential targets of Xinyi San were got, and the target genes of rhinitis of Xinyi San were extracted from GeneCards databases. Then we constructed protein-protein interactions (PPI) network of target genes, and then analyzed the Key genes in GO analysis and KEGG analysis. Results: We got 97 components, 53 potential therapeutic targets, 1009 GO items and 92 pathways in our study. The main pathways included Lipid and atherosclerosis, Chemical carcinogenesis-receptor activation, PI3K-Akt signaling pathway, Human cytomegalovirus infection, Prostate cancer, etc. Conclusion: Xinyi San plays a role in treating rhinitis through multiple components, multiple targets and multiple pathways.
基金This work was supported by the Science Foundation of Heilongjiang Administration of Traditional Chinese Medicine(No.ZHY18-021).
文摘In recent years,microbial metabolomics,a new field that has attracted wide attention,provides a map of metabolic pathways and clarifies the interaction mechanism between microorganisms and hosts.Many microorganisms are found in the human intestine,oral cavity,vagina,etc.Probiotics could maintain the good health of the host,while pathogens and an imbalance of bacterial flora lead to a series of diseases of the body and mind.Metabolomics is a science for qualitative and quantitative analysis of all metabolites in an organism or biological system,which could provide key information to understand the related metabolic pathways and associated changes.This approach analyzes the final products of cellular regulatory processes,the level of which can be regarded as the ultimate response of the biological system to genetic or environmental changes.Microbial metabolomics has been widely used in different research fields,such as microbial phenotypic classification,mutant screening,metabolic pathways,microbial metabolic engineering,fermentation engineering monitoring and optimization,microbial environmental pollution,and so on.However,there are only a few reviews on microbial metabolomics of probiotics and pathogens.This review summarizes the main methodologies,including sample preparation,identification of metabolites,data processing,and analysis.Recent applications in microbial metabolomics of probiotics and pathogens are also described.This paper first summarized the research progress and application of microbial metabolomics from two aspects:probiotics and pathogenic bacteria.Probiotics and pathogenic bacteria do not exist independently most of the time;hence,these were reviewed in the research field of coexistence of probiotics and pathogenic bacteria,which was subdivided into important microbial research fields closely related to human health,including the human gut,oral cavity,food,and nutrition-related microorganisms.Then,the main problems and trends associated with microbial metabolomics are discussed.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.62005108 and 62205134)the National Key Research and Development Program of China(Grant No.2022YFC2807701)the Natural Science Foundation of the Higher Education Institutions of Jiangsu Province,China(Grant Nos.20KJB140009 and 21KJB140008)。
文摘This study established a novel method for the simultaneous detection of two-component gases.Radio frequency(RF)white noise disturbance laser current and wavelength modulation were simultaneously used to improve the off-axis integrated cavity output spectroscopy technique,and a high-precision dual modulation OA-ICOS(RF-WM-OA-ICOS)system was established.The two laser beams were coupled into one laser beam that was applied incident to the cavity of RF-WM-OA-ICOS system.The second harmonic signals of CH_(4)and CO_(2)gas simultaneously appeared in the rising or falling edge of a triangular wave.This method was used to measure CH_(4)and CO_(2)with different concentrations.The results indicated that the proposed system has high stability and can accurately and simultaneously measure the concentrations of CH_(4)and CO_(2),with an optimal integration time of 220 s.The minimum detection limit was 10 ppb for CH_(4)and 1.5 ppm for CO_(2).The corresponding noise equivalent absorption sensitivity values were calculated as 2.67×10^(-13)cm^(-1)·Hz^(-1/2)and 5.18×10^(-11)cm^(-1)·Hz^(-1/2),respectively.The proposed dual-component gas simultaneous detection method can also be used for high-precision simultaneous detection of other gases.Therefore,this study may serve as a reference for developing portable multicomponent gas analyzers.
基金Supported by Youth Project of National Natural Science Foundation of China(81501080)Funding Project for Introducing Overseas Students in Hebei Provincial Department of Human Resources and Social Security(C20190174)Construction of Chengde Biomedical Industry Research Institute(202205B086)。
文摘[Objectives]To investigate the spasticity of rat tail and the expression of dopamine receptor-1(DRD1)mRNA in the spinal cord after spinal cord injury(SCI)induced tail spasticity in rats.[Methods]Adult male Wistar rats were randomly divided into Sham group and SCI group.The second sacral spinal cord(S2)segment of SCI rats was completely transected.60 d after operation,the rat tail spasticity was scored,and then the spinal cord tissues below the level of S2 spinal cord transection were taken.The expression of DRD1 mRNA in the sacrococcygeal spinal cord was detected by qPCR.In addition,3 normal rats were used for DAR/neuronal nuclei(NeuN)and DRD1/choline acetyltransferase(ChAT)immunofluorescence staining to study the distribution of DRD1 in spinal cord and the properties of DRD1 positive cells.[Results]60 d after operation in SCI group,the tail spasticity of rats developed fully,and the symptoms of spasticity were typical.qPCR results showed that the expression of DRD1 mRNA in SCI group was significantly lower than that in Sham group(P<0.05).DRD1 was widely distributed in the dorsal horn,intermediate zone and ventral horn at the sacrococcygeal end of the rat spinal cord.[Conclusions]The decrease of DRD1 mRNA expression after SCI may be related to the occurrence and development of spasticity.
文摘采用硅烷偶联剂Z6020对六方氮化硼(h-BN)进行改性,通过原位聚合法制备聚酰亚胺/改性氮化硼(PI/f-BN)复合薄膜。利用红外光谱仪、自制调频耐电晕装置和宽频介电谱测试仪对复合薄膜进行测试,采用光学显微镜对薄膜耐电晕老化后的击穿孔形貌进行表征。结果表明:在棒板空气间隙为1 mm,脉冲电场强度为50 k V/mm,占空比为50%,频率为20 k Hz的测试条件下,随着f-BN含量的提高,薄膜的耐电晕寿命呈先增加后缩短的趋势。当f-BN含量为15%时,复合薄膜的介电常数最大,耐电晕寿命最长。
基金supported by the Innovation and Entrepreneurship Training Program Topic for College Students of North China University of Technology in 2023.
文摘In order to enhance modeling efficiency and accuracy,we utilized 3D laser point cloud data for indoor space modeling.Point cloud data was obtained with a 3D laser scanner and optimized with Autodesk Recap and Revit software to extract geometric information about the indoor environment.Furthermore,we proposed a method for constructing indoor elements based on parametric components.The research outcomes of this paper will offer new methods and tools for indoor space modeling and design.The approach of indoor space modeling based on 3D laser point cloud data and parametric component construction can enhance modeling efficiency and accuracy,providing architects,interior designers,and decorators with a better working platform and design reference.
基金the support of the Innovation and Entrepreneurship Training Program Topic for College Students of North China University of Technology in 2023.
文摘Requirements for the quality of housing have been increasing in recent years.Many existing buildings can no longer meet the needs of residents due to functional,layout,and other issues,and need to be renovated.However,traditional renovation methods have problems such as high pollution and high consumption.With the rapid development of prefabricated construction and green environmental protection concepts,the problems of traditional interior renovation can be resolved using prefabricated interior decoration.As a new and advanced method of renovation,prefabricated renovation reduces construction segments,workload,pollution,and waste,and it promotes the upgrading of the traditional renovation industry.
