Background:The interrelation between intestinal polyps,metabolic syndrome(MetS),and colorectal cancer(CRC)is a critical area of study.This research focuses on pinpointing potential molecular targets to understand the ...Background:The interrelation between intestinal polyps,metabolic syndrome(MetS),and colorectal cancer(CRC)is a critical area of study.This research focuses on pinpointing potential molecular targets to understand the link between intestinal polyp formation,metabolic irregularities,and CRC progression.Methods:We examined clinical samples from patients with intestinal polyps coexisting with MetS and compared them with samples from patients with standard intestinal polyps.Transcriptome sequencing and public database analysis were employed to identify significant pathways and genes.These targets were then validated through immunohistochemistry(IHC).Following the RNA interference of key target expression,a series of experiments,including the cell counting kit-8 assay,colony formation,wound healing,and Transwell assays,were conducted.Results:Comparative analysis revealed 75 up-regulated and 61 down-regulated differentially expressed genes(DEGs)in the MetS polyp group vs.the control.Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment suggested these DEGs were primarily associated with cell cycle and mitosis.Integration with comparative toxicogenomics database(CTD)and the cancer genome atlas(TCGA)databases highlighted 44 key CRC-related genes.Protein interaction networks indicated connections of purkinje cell protein 4(PCP4),olfactomedin 1(OLFM1),fibronectin 1(FN1),and transforming growth factor beta 3(TGF-β3)with the mitogen-activated protein kinase(MAPK)pathway.Tumor correlation studies suggested higher risk associations with FN1,PCP4,and TGF-β3,while OLFM1 was identified as a lower risk gene.Immunohistochemical analysis revealed a decrease in OLFM1 in MetS-associated intestinal polyps.Upon interference with OLFM1 in polyp epithelial cells,there was a significant enhancement in cell proliferation,colony formation,and cell migration and invasion capabilities.Conclusion:Our study highlights a significant decrease in OLFM1 expression in MetS-associated intestinal polyps.And,this reduction in OLFM1 is associated with enhanced cell proliferation,colony formation,and increased cell migration and invasion capabilities.These findings underscore the reduced OLFM1 expression in MetS-associated intestinal polyps may play a crucial role in promoting tumorigenic processes in colorectal pathology.Further research on OLFM1 may provide valuable insights into understanding and targeting MetS-associated intestinal polyps.展开更多
With the rapid development of information technology and the Internet,people can easily and quickly obtain news and information from all over the world.As the state’s requirements for the curriculum ideology and poli...With the rapid development of information technology and the Internet,people can easily and quickly obtain news and information from all over the world.As the state’s requirements for the curriculum ideology and politics are being strengthened,it is necessary to adapt to the needs of the times and integrate ideology and politics into college English teaching.Integration of ideology and politics into teaching is a challenge faced by colleges and universities nowadays,which aims to make ideology and politics alive and well and more acceptable to contemporary college students.In view of this,this paper starts with the importance of the integration of ideology and politics into college English teaching,takes An Overview of Chinese Culture as an example,analyzes new approaches for integrating ideology and politics into the teaching process,and formulates the teaching design centered around these approaches,so as to promote the organic integration of ideology and politics with English teaching.展开更多
Micro-expression recognition has attracted growing research interests in the field of compute vision.However,micro-expression usually lasts a few seconds,thus it is difficult to detect.This paper presents a new framew...Micro-expression recognition has attracted growing research interests in the field of compute vision.However,micro-expression usually lasts a few seconds,thus it is difficult to detect.This paper presents a new framework to recognize micro-expression using pyramid histogram of Centralized Gabor Binary Pattern from Three Orthogonal Panels(CGBP-TOP)which is an extension of Local Gabor Binary Pattern from Three Orthogonal Panels feature.CGBP-TOP performs spatial and temporal analysis to capture the local facial characteristics of micro-expression image sequences.In order to keep more local information of the face,CGBP-TOP is extracted based on pyramid subregions of the micro-expression video frame.The combination of CGBP-TOP and spatial pyramid can represent well and truly the facial movements of the micro-expression image sequences.However,the dimension of our pyramid CGBP-TOP tends to be very high,which may lead to high data redundancy problem.In addition,it is clear that people of different genders usually have different ways of micro-expression.Therefore,in this paper,in order to select the relevant features of micro-expression,the gender-specific sparse multi-task learning method with adaptive regularization term is adopted to learn a compact subset of pyramid CGBP-TOP feature for micro-expression classification of different sexes.Finally,extensive experiments on widely used CASME II and SMIC databases demonstrate that our method can efficiently extract micro-expression motion features in the micro-expression video clip.Moreover,our proposed approach achieves comparable results with the state-of-the-art methods.展开更多
Hepatic lipid and fatty acid(FA)metabolism are critical for regulating energetic homeostasis during embryogenesis.At present,it remains unclear how an exogenous FA intervention affects embryonic development in an avia...Hepatic lipid and fatty acid(FA)metabolism are critical for regulating energetic homeostasis during embryogenesis.At present,it remains unclear how an exogenous FA intervention affects embryonic development in an avian embryo model.In Exp.1,30 fertilized eggs were sampled on embryonic days(E)16,19,22,25,28,31 and the day of hatch(DOH)to determine the critical period of lipid metabolism.In Exp.2,a total of 120 fertilized eggs were divided into two groups(60 eggs/group)for in ovo feeding(IOF)procedures on E25.Eggs were injected into the yolk sac with PBS as the control group and with oleic acid(OA)as the IOF-OA treatment group.Samples were collected on E28 and E31.In Exp.1,hepatic triacylglycerol(TG)and cholesterol(CHO)contents increased while serum TG content decreased from E16 to DOH(P<0.05).Both serum and liver displayed an increase in unsaturated FA and a decrease in saturated FA(P<0.05).There was a quadratic increase in the target gene and protein expression related to hepatic FA de novo synthesis and oxidation(P<0.05),whose inflection period was between E22 and E28.In Exp.2,compared with the control embryos,IOF-OA embryos had an increased yolk sac TG content on E28 and E31,and a decreased serum TG and CHO content on E28(P<0.05).The IOF-OA embryos had less OA in the yolk sac and liver on E28,and less unsaturated FA in the serum and liver on E31 than did the control embryos(P<0.05).Hepatic gene mRNA expression related to FA uptake,synthesis,and oxidation on E28 was lower in IOF-OA than in control embryos(P<0.05),not on E31(P>0.05).Maximal metabolic changes in lipid and FA metabolism occurred on E22-E28 in Muscovy duck embryogenesis,along with the altered target gene and protein expression related to lipogenesis and lipolysis.IOF-OA intervention on E25 could inhibit the target gene expression related to FA uptake,synthesis,and oxidation,which may influence the normal FA metabolism on E28 during embryogenesis.展开更多
Porous polyurethane concrete(PPUC)is a novel material for permeable pavements and is considered as an alternative to porous asphalt or porous cement concrete.However,studies of the mechanical properties of PPUC are st...Porous polyurethane concrete(PPUC)is a novel material for permeable pavements and is considered as an alternative to porous asphalt or porous cement concrete.However,studies of the mechanical properties of PPUC are still insufficient.In this study,the comprehensive mechanical properties and water stability of PPUC with different gradations and polyurethane dosages were investigated,and its water damage mechanism was preliminarily explored.The results show that the flexural strength and Marshall stability of PPUC can more easily reach the index in the standards of porous cement concrete or porous asphalt,while the compressive strength and abrasion resistance are the weak points of its mechanical properties and need to be further optimized.The mechanical properties and water stability of PPUC were effectively improved by increasing the polyurethane dosage and using continuously graded aggregates.PPUC is more susceptible to water damage because water reacts with the residual isocyanate groups within the polyurethane film to generate carbon dioxide gas,which reduces the cohesion and adhesion performance of polyurethane film.This study provides a comprehensive understanding of the mechanical properties of PPUC and an initial insight into the mechanism of water damage.展开更多
基金This work was supported by Hospital Incubation Fund of the First Affiliated Hospital of Chongqing Medical and Pharmaceutical College(No.201704).
文摘Background:The interrelation between intestinal polyps,metabolic syndrome(MetS),and colorectal cancer(CRC)is a critical area of study.This research focuses on pinpointing potential molecular targets to understand the link between intestinal polyp formation,metabolic irregularities,and CRC progression.Methods:We examined clinical samples from patients with intestinal polyps coexisting with MetS and compared them with samples from patients with standard intestinal polyps.Transcriptome sequencing and public database analysis were employed to identify significant pathways and genes.These targets were then validated through immunohistochemistry(IHC).Following the RNA interference of key target expression,a series of experiments,including the cell counting kit-8 assay,colony formation,wound healing,and Transwell assays,were conducted.Results:Comparative analysis revealed 75 up-regulated and 61 down-regulated differentially expressed genes(DEGs)in the MetS polyp group vs.the control.Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment suggested these DEGs were primarily associated with cell cycle and mitosis.Integration with comparative toxicogenomics database(CTD)and the cancer genome atlas(TCGA)databases highlighted 44 key CRC-related genes.Protein interaction networks indicated connections of purkinje cell protein 4(PCP4),olfactomedin 1(OLFM1),fibronectin 1(FN1),and transforming growth factor beta 3(TGF-β3)with the mitogen-activated protein kinase(MAPK)pathway.Tumor correlation studies suggested higher risk associations with FN1,PCP4,and TGF-β3,while OLFM1 was identified as a lower risk gene.Immunohistochemical analysis revealed a decrease in OLFM1 in MetS-associated intestinal polyps.Upon interference with OLFM1 in polyp epithelial cells,there was a significant enhancement in cell proliferation,colony formation,and cell migration and invasion capabilities.Conclusion:Our study highlights a significant decrease in OLFM1 expression in MetS-associated intestinal polyps.And,this reduction in OLFM1 is associated with enhanced cell proliferation,colony formation,and increased cell migration and invasion capabilities.These findings underscore the reduced OLFM1 expression in MetS-associated intestinal polyps may play a crucial role in promoting tumorigenic processes in colorectal pathology.Further research on OLFM1 may provide valuable insights into understanding and targeting MetS-associated intestinal polyps.
文摘With the rapid development of information technology and the Internet,people can easily and quickly obtain news and information from all over the world.As the state’s requirements for the curriculum ideology and politics are being strengthened,it is necessary to adapt to the needs of the times and integrate ideology and politics into college English teaching.Integration of ideology and politics into teaching is a challenge faced by colleges and universities nowadays,which aims to make ideology and politics alive and well and more acceptable to contemporary college students.In view of this,this paper starts with the importance of the integration of ideology and politics into college English teaching,takes An Overview of Chinese Culture as an example,analyzes new approaches for integrating ideology and politics into the teaching process,and formulates the teaching design centered around these approaches,so as to promote the organic integration of ideology and politics with English teaching.
基金This work is funded by the natural science foundation of Jiangsu Province(No.BK20150471)the natural science foundation of the higher education institutions of Jiangsu Province(No.17KJB520007)+2 种基金the Key Research and Development Program of Zhenjiang-Social Development(No.SH2018005)the scientific researching fund of Jiangsu University of Science and Technology(No.1132921402,No.1132931803)the basic science and frontier technology research program of Chongqing Municipal Science and Technology Commission(cstc2016jcyjA0407).
文摘Micro-expression recognition has attracted growing research interests in the field of compute vision.However,micro-expression usually lasts a few seconds,thus it is difficult to detect.This paper presents a new framework to recognize micro-expression using pyramid histogram of Centralized Gabor Binary Pattern from Three Orthogonal Panels(CGBP-TOP)which is an extension of Local Gabor Binary Pattern from Three Orthogonal Panels feature.CGBP-TOP performs spatial and temporal analysis to capture the local facial characteristics of micro-expression image sequences.In order to keep more local information of the face,CGBP-TOP is extracted based on pyramid subregions of the micro-expression video frame.The combination of CGBP-TOP and spatial pyramid can represent well and truly the facial movements of the micro-expression image sequences.However,the dimension of our pyramid CGBP-TOP tends to be very high,which may lead to high data redundancy problem.In addition,it is clear that people of different genders usually have different ways of micro-expression.Therefore,in this paper,in order to select the relevant features of micro-expression,the gender-specific sparse multi-task learning method with adaptive regularization term is adopted to learn a compact subset of pyramid CGBP-TOP feature for micro-expression classification of different sexes.Finally,extensive experiments on widely used CASME II and SMIC databases demonstrate that our method can efficiently extract micro-expression motion features in the micro-expression video clip.Moreover,our proposed approach achieves comparable results with the state-of-the-art methods.
基金This study was sponsored by National Natural Science Foundation of China(31972584)China Agriculture Research System of MOF and MARA(CARS-42-15).
文摘Hepatic lipid and fatty acid(FA)metabolism are critical for regulating energetic homeostasis during embryogenesis.At present,it remains unclear how an exogenous FA intervention affects embryonic development in an avian embryo model.In Exp.1,30 fertilized eggs were sampled on embryonic days(E)16,19,22,25,28,31 and the day of hatch(DOH)to determine the critical period of lipid metabolism.In Exp.2,a total of 120 fertilized eggs were divided into two groups(60 eggs/group)for in ovo feeding(IOF)procedures on E25.Eggs were injected into the yolk sac with PBS as the control group and with oleic acid(OA)as the IOF-OA treatment group.Samples were collected on E28 and E31.In Exp.1,hepatic triacylglycerol(TG)and cholesterol(CHO)contents increased while serum TG content decreased from E16 to DOH(P<0.05).Both serum and liver displayed an increase in unsaturated FA and a decrease in saturated FA(P<0.05).There was a quadratic increase in the target gene and protein expression related to hepatic FA de novo synthesis and oxidation(P<0.05),whose inflection period was between E22 and E28.In Exp.2,compared with the control embryos,IOF-OA embryos had an increased yolk sac TG content on E28 and E31,and a decreased serum TG and CHO content on E28(P<0.05).The IOF-OA embryos had less OA in the yolk sac and liver on E28,and less unsaturated FA in the serum and liver on E31 than did the control embryos(P<0.05).Hepatic gene mRNA expression related to FA uptake,synthesis,and oxidation on E28 was lower in IOF-OA than in control embryos(P<0.05),not on E31(P>0.05).Maximal metabolic changes in lipid and FA metabolism occurred on E22-E28 in Muscovy duck embryogenesis,along with the altered target gene and protein expression related to lipogenesis and lipolysis.IOF-OA intervention on E25 could inhibit the target gene expression related to FA uptake,synthesis,and oxidation,which may influence the normal FA metabolism on E28 during embryogenesis.
基金supported by the Fundamental Research Funds for the Central Universities (No.22120210027).
文摘Porous polyurethane concrete(PPUC)is a novel material for permeable pavements and is considered as an alternative to porous asphalt or porous cement concrete.However,studies of the mechanical properties of PPUC are still insufficient.In this study,the comprehensive mechanical properties and water stability of PPUC with different gradations and polyurethane dosages were investigated,and its water damage mechanism was preliminarily explored.The results show that the flexural strength and Marshall stability of PPUC can more easily reach the index in the standards of porous cement concrete or porous asphalt,while the compressive strength and abrasion resistance are the weak points of its mechanical properties and need to be further optimized.The mechanical properties and water stability of PPUC were effectively improved by increasing the polyurethane dosage and using continuously graded aggregates.PPUC is more susceptible to water damage because water reacts with the residual isocyanate groups within the polyurethane film to generate carbon dioxide gas,which reduces the cohesion and adhesion performance of polyurethane film.This study provides a comprehensive understanding of the mechanical properties of PPUC and an initial insight into the mechanism of water damage.
