Anaplastic thyroid carcinoma(ATC)is a rare but extremely lethal malignancy.However,little is known about the pathogenesis of ATC.Given its high mortality,it is critical to improve our understanding of ATC pathogenesis...Anaplastic thyroid carcinoma(ATC)is a rare but extremely lethal malignancy.However,little is known about the pathogenesis of ATC.Given its high mortality,it is critical to improve our understanding of ATC pathogenesis and to find new diagnostic biomarkers.In the present study,two gene microarray profiles(GSE53072 and GSE65144),which included 17 ATC and 17 adjacent non-tumorous tissues,were obtained.Bioinformatic analyses were then performed.Immunohistochemistry(IHC)and receiver operating characteristic(ROC)curves were then used to detect transmembrane protein 158(TMEM158)expression and to assess diagnostic sensitivity.A total of 372 differentially expressed genes(DEGs)were identified.Through protein-protein interaction(PPI)analysis,we identified a significant module with 37 upregulated genes.Most of the genes in this module were related to cell-cycle processes.After co-expression analysis,132 hub genes were selected for further study.Nine genes were identified as both DEGs and genes of interest in the weighted gene co-expression network analysis(WGCNA).IHC and ROC curves confirmed that TMEM158 was overexpressed in ATC tissue as compared with other types of thyroid cancer and normal tissue samples.We identified 8 KEGG pathways that were associated with high expression of TMEM158,including aminoacyl-tRNA biosynthesis and DNA replication.Our results suggest that TMEM158 may be a potential oncogene and serve as a diagnostic indicator for ATC.展开更多
The method for batch continuous production of polymerized cholesteric liquid crystal(PCLC) microdisks based on centrifugal microfluidics was proposed. The prototype centrifugal microfluidic chip was fabricated by the ...The method for batch continuous production of polymerized cholesteric liquid crystal(PCLC) microdisks based on centrifugal microfluidics was proposed. The prototype centrifugal microfluidic chip was fabricated by the wetetching technique with a series of ladder-like concentric ring channels whose depths decrease radially. Two types of dye-doped PCLC microdisks with different doping procedures were generated by this method, the tunable lasing properties were characterized, and corresponding potential applications in a micro-cavity laser and optical barcode were demonstrated. The method is also applicable to a broad range of other polymerized materials.展开更多
基金This study was supported by grants from Tongji Medical College,Huazhong University of Science and Technology(CN)(No.5001540018)Young Scientists Fund(No.81802676).
文摘Anaplastic thyroid carcinoma(ATC)is a rare but extremely lethal malignancy.However,little is known about the pathogenesis of ATC.Given its high mortality,it is critical to improve our understanding of ATC pathogenesis and to find new diagnostic biomarkers.In the present study,two gene microarray profiles(GSE53072 and GSE65144),which included 17 ATC and 17 adjacent non-tumorous tissues,were obtained.Bioinformatic analyses were then performed.Immunohistochemistry(IHC)and receiver operating characteristic(ROC)curves were then used to detect transmembrane protein 158(TMEM158)expression and to assess diagnostic sensitivity.A total of 372 differentially expressed genes(DEGs)were identified.Through protein-protein interaction(PPI)analysis,we identified a significant module with 37 upregulated genes.Most of the genes in this module were related to cell-cycle processes.After co-expression analysis,132 hub genes were selected for further study.Nine genes were identified as both DEGs and genes of interest in the weighted gene co-expression network analysis(WGCNA).IHC and ROC curves confirmed that TMEM158 was overexpressed in ATC tissue as compared with other types of thyroid cancer and normal tissue samples.We identified 8 KEGG pathways that were associated with high expression of TMEM158,including aminoacyl-tRNA biosynthesis and DNA replication.Our results suggest that TMEM158 may be a potential oncogene and serve as a diagnostic indicator for ATC.
基金supported by the National Natural Science Foundation of China(NSFC)(No.61675172)the Natural Science Foundation of Fujian Province,China(No.2017J01124)。
文摘The method for batch continuous production of polymerized cholesteric liquid crystal(PCLC) microdisks based on centrifugal microfluidics was proposed. The prototype centrifugal microfluidic chip was fabricated by the wetetching technique with a series of ladder-like concentric ring channels whose depths decrease radially. Two types of dye-doped PCLC microdisks with different doping procedures were generated by this method, the tunable lasing properties were characterized, and corresponding potential applications in a micro-cavity laser and optical barcode were demonstrated. The method is also applicable to a broad range of other polymerized materials.
