Fusobacterium nucleatum(F.nucleatum)is an early pathogenic colonizer in periodontitis,but the host response to infection with this pathogen remains unclear.In this study,we built an F.nucleatum infectious model with h...Fusobacterium nucleatum(F.nucleatum)is an early pathogenic colonizer in periodontitis,but the host response to infection with this pathogen remains unclear.In this study,we built an F.nucleatum infectious model with human periodontal ligament stem cells(PDLSCs)and showed that F.nucleatum could inhibit proliferation,and facilitate apoptosis,ferroptosis,and inflammatory cytokine production in a dose-dependent manner.The F.nucleatum adhesin Fad A acted as a proinflammatory virulence factor and increased the expression of interleukin(IL)-1β,IL-6 and IL-8.Further study showed that Fad A could bind with PEBP1 to activate the Raf1-MAPK and IKK-NF-κB signaling pathways.Time-course RNA-sequencing analyses showed the cascade of gene activation process in PDLSCs with increasing durations of F.nucleatum infection.NFκB1 and NFκB2 upregulated after 3 h of F.nucleatum-infection,and the inflammatory-related genes in the NF-κB signaling pathway were serially elevated with time.Using computational drug repositioning analysis,we predicted and validated that two potential drugs(piperlongumine and fisetin)could attenuate the negative effects of F.nucleatum-infection.Collectively,this study unveils the potential pathogenic mechanisms of F.nucleatum and the host inflammatory response at the early stage of F.nucleatum infection.展开更多
Objective:To compare the therapeutic effect difference among dog-day moxibustion on herbs,ordinary moxibution and routine western medication in treating knee-joint osteoarthritis of yang deficiency and congealing cold...Objective:To compare the therapeutic effect difference among dog-day moxibustion on herbs,ordinary moxibution and routine western medication in treating knee-joint osteoarthritis of yang deficiency and congealing cold type.Methods:A total of 90 patients with knee-joint osteoarthritis of yang deficiency and congealing cold type were divided into dog-day moxibustion group,ordinary moxibution group and western medication group according to the registration order,and there were 30 patients in each group.Among them,the patients in the dog-day moxibustion were given dog-day moxibustion on herbs method during the dog days of 2018(July 17 to August 25).Continuous treatment was applied on the first,second and third dog-day with 5 days for each dog-day,and once for a day,and there were totally 15 times of treatment.In the same period,the patients in the ordinary moxibustion group was treated with the pure moxa stick moxibustion,and the point selection and treatment course were the same as the dog-day moxibustion group.And in the same period,the patients in the western medication group were treated with routine western medication,and the patient took orally diclofenac sodium sustained-release capsules,with 100 mg each time,once a day.The Visual Analog Scale(VAS),Western Ontario and McMaster University Arthritis Index(WOMAC),life quality score and clinical efficacy for the knee joint of the three groups before and after treatment were observed.Results:After treatment,the effective rate of the dog-day moxibustion was 93.33%,the effective rate of the ordinary moxibustion group was 83.33%,and the effective rate of the western medication group was 53.33%.Compared with pre-treatment,the VAS scores and WOMAC scores of the patients with KOA in the three groups after treatment decreased,and the differences were statistically significant(all P<0.05).There were also statistically significant differences between the VAS score,total WOMAC score,pain score,joint stiffness score and joint function score of the three groups after treatment(all P<0.05).Conclusion:The effect of dog-day moxibustion on herbs in treating knee-joint osteoarthritis of yang deficiency and congealing cold type was significantly better than ordinary moxibustion and western medication,and it was worthy of clinical promotion.展开更多
基金foundation support of the National Natural Science Foundation of China(Grant No.82071122)the Program of Taishan Young from Shandong Province+3 种基金Major Innovation Projects in Shandong Province(No.2021SFGC0502)Oral Microbiome Innovation Team of Young Scientist Project of Shandong Province(Grant No.2020KJK001)and Jinan City(2021GXRC021)The National High-level Young Scientist Project Foundation(2019)Excellent Young Scientist Foundation of Shandong Province(Grant No.ZR202102230369)。
文摘Fusobacterium nucleatum(F.nucleatum)is an early pathogenic colonizer in periodontitis,but the host response to infection with this pathogen remains unclear.In this study,we built an F.nucleatum infectious model with human periodontal ligament stem cells(PDLSCs)and showed that F.nucleatum could inhibit proliferation,and facilitate apoptosis,ferroptosis,and inflammatory cytokine production in a dose-dependent manner.The F.nucleatum adhesin Fad A acted as a proinflammatory virulence factor and increased the expression of interleukin(IL)-1β,IL-6 and IL-8.Further study showed that Fad A could bind with PEBP1 to activate the Raf1-MAPK and IKK-NF-κB signaling pathways.Time-course RNA-sequencing analyses showed the cascade of gene activation process in PDLSCs with increasing durations of F.nucleatum infection.NFκB1 and NFκB2 upregulated after 3 h of F.nucleatum-infection,and the inflammatory-related genes in the NF-κB signaling pathway were serially elevated with time.Using computational drug repositioning analysis,we predicted and validated that two potential drugs(piperlongumine and fisetin)could attenuate the negative effects of F.nucleatum-infection.Collectively,this study unveils the potential pathogenic mechanisms of F.nucleatum and the host inflammatory response at the early stage of F.nucleatum infection.
基金Supported by Research Projects of Traditional Chinese Medicine of Hubei Health and Family Planning Commission: ZY2019M035Wuhan Clinical Medical Research Project: WZ14A01
文摘Objective:To compare the therapeutic effect difference among dog-day moxibustion on herbs,ordinary moxibution and routine western medication in treating knee-joint osteoarthritis of yang deficiency and congealing cold type.Methods:A total of 90 patients with knee-joint osteoarthritis of yang deficiency and congealing cold type were divided into dog-day moxibustion group,ordinary moxibution group and western medication group according to the registration order,and there were 30 patients in each group.Among them,the patients in the dog-day moxibustion were given dog-day moxibustion on herbs method during the dog days of 2018(July 17 to August 25).Continuous treatment was applied on the first,second and third dog-day with 5 days for each dog-day,and once for a day,and there were totally 15 times of treatment.In the same period,the patients in the ordinary moxibustion group was treated with the pure moxa stick moxibustion,and the point selection and treatment course were the same as the dog-day moxibustion group.And in the same period,the patients in the western medication group were treated with routine western medication,and the patient took orally diclofenac sodium sustained-release capsules,with 100 mg each time,once a day.The Visual Analog Scale(VAS),Western Ontario and McMaster University Arthritis Index(WOMAC),life quality score and clinical efficacy for the knee joint of the three groups before and after treatment were observed.Results:After treatment,the effective rate of the dog-day moxibustion was 93.33%,the effective rate of the ordinary moxibustion group was 83.33%,and the effective rate of the western medication group was 53.33%.Compared with pre-treatment,the VAS scores and WOMAC scores of the patients with KOA in the three groups after treatment decreased,and the differences were statistically significant(all P<0.05).There were also statistically significant differences between the VAS score,total WOMAC score,pain score,joint stiffness score and joint function score of the three groups after treatment(all P<0.05).Conclusion:The effect of dog-day moxibustion on herbs in treating knee-joint osteoarthritis of yang deficiency and congealing cold type was significantly better than ordinary moxibustion and western medication,and it was worthy of clinical promotion.
