Retinal neurodegenerative disease is a leading cause of blindness among the elderly in developed countries,including glaucoma,diabetic retinopathy,traumatic optic neuropathy and optic neuritis,etc.The current clinical...Retinal neurodegenerative disease is a leading cause of blindness among the elderly in developed countries,including glaucoma,diabetic retinopathy,traumatic optic neuropathy and optic neuritis,etc.The current clinical treatment is not very effective.We investigated indirubin,one of the main bioactive components of the traditional Chinese medicine Danggui Longhui Pill,in the present study for its role in retinal neurodegeneration.Indirubin exhibited no detectable tissue toxicity in vivo or cytotoxicity in vitro.Moreover,indirubin improved visual function and ameliorated retinal neurodegeneration in mice after optic nerve crush injury in vivo.Furthermore,indirubin reduced the apoptosis of retinal ganglion cells induced by oxidative stress in vitro.In addition,indirubin significantly suppressed the increased production of intracellular reactive oxygen species and the decreased activity of superoxide dismutase induced by oxidative stress.Mechanically,indirubin played a neuroprotective role by regulating the PI3K/AKT/BAD/BCL-2 signaling.In conclusion,indirubin protected retinal ganglion cells from oxidative damage and alleviated retinal neurodegeneration induced by optic nerve crush injury.The present study provides a potential therapeutic medicine for retinal neurodegenerative diseases.展开更多
Pericytes are located in the stromal membrane of the capillary outer wall and contain endothelial cells.They are pivotal in regulating blood flow,enhancing vascular stability,and maintaining the integrity of the blood...Pericytes are located in the stromal membrane of the capillary outer wall and contain endothelial cells.They are pivotal in regulating blood flow,enhancing vascular stability,and maintaining the integrity of the blood-retina barrier/blood-brain barrier.The pluripotency of pericytes allows them to differentiate into various cell types,highlighting their significance in vascular disease pathogenesis,as demonstrated by previous studies.This capability enables pericytes to be a potential biomarker for the diagnosis and a target for the treatment of vascular disorders.The retina,an essential part of the eyeball,is an extension of cerebral tissue with a transparent refractive medium.It offers a unique window for assessing systemic microvascular lesions.Routine fundus examination is necessary for patients with diabetes and hypertension.Manifestations,such as retinal artery tortuosity,dilation,stenosis,and abnormal arteriovenous anastomosis,serve as typical hallmarks of retinal vasculopathy.Therefore,studies of ocular vascular diseases significantly facilitate the exploration of systemic vascular diseases.展开更多
基金supported by grants from the National Natural Science Foundation of China(Grant Nos.81970823 and 82271107)the Natural Science Foundation of Jiangsu Province(Grant No.BK20221186).
文摘Retinal neurodegenerative disease is a leading cause of blindness among the elderly in developed countries,including glaucoma,diabetic retinopathy,traumatic optic neuropathy and optic neuritis,etc.The current clinical treatment is not very effective.We investigated indirubin,one of the main bioactive components of the traditional Chinese medicine Danggui Longhui Pill,in the present study for its role in retinal neurodegeneration.Indirubin exhibited no detectable tissue toxicity in vivo or cytotoxicity in vitro.Moreover,indirubin improved visual function and ameliorated retinal neurodegeneration in mice after optic nerve crush injury in vivo.Furthermore,indirubin reduced the apoptosis of retinal ganglion cells induced by oxidative stress in vitro.In addition,indirubin significantly suppressed the increased production of intracellular reactive oxygen species and the decreased activity of superoxide dismutase induced by oxidative stress.Mechanically,indirubin played a neuroprotective role by regulating the PI3K/AKT/BAD/BCL-2 signaling.In conclusion,indirubin protected retinal ganglion cells from oxidative damage and alleviated retinal neurodegeneration induced by optic nerve crush injury.The present study provides a potential therapeutic medicine for retinal neurodegenerative diseases.
基金supported by grants from the National Natural Science Foundation of China(Grant No.82070983)the Natural Science Foundation of Jiangsu Province(Grant No.BK20211020)as well as grants from the Medical Science and Technology Development Project Fund of Nanjing(Grant No.YKK22263).
文摘Pericytes are located in the stromal membrane of the capillary outer wall and contain endothelial cells.They are pivotal in regulating blood flow,enhancing vascular stability,and maintaining the integrity of the blood-retina barrier/blood-brain barrier.The pluripotency of pericytes allows them to differentiate into various cell types,highlighting their significance in vascular disease pathogenesis,as demonstrated by previous studies.This capability enables pericytes to be a potential biomarker for the diagnosis and a target for the treatment of vascular disorders.The retina,an essential part of the eyeball,is an extension of cerebral tissue with a transparent refractive medium.It offers a unique window for assessing systemic microvascular lesions.Routine fundus examination is necessary for patients with diabetes and hypertension.Manifestations,such as retinal artery tortuosity,dilation,stenosis,and abnormal arteriovenous anastomosis,serve as typical hallmarks of retinal vasculopathy.Therefore,studies of ocular vascular diseases significantly facilitate the exploration of systemic vascular diseases.
