The impact of alkyl dimethyl betaine (ADB) on the collection capacity of sodium oleate (NaOl) at low temperatures was evaluated using flotation tests at various scales. The low-temperature synergistic mechanism of ADB...The impact of alkyl dimethyl betaine (ADB) on the collection capacity of sodium oleate (NaOl) at low temperatures was evaluated using flotation tests at various scales. The low-temperature synergistic mechanism of ADB and NaOl was explored by infrared spectroscopy, X-ray photoelectron spectroscopy, surface tension measurement, foam performance test, and flotation reagent size measurement.The flotation tests revealed that the collector mixed with octadecyl dimethyl betaine (ODB) and NaOl in a mass ratio of 4:96 exhibited the highest collection capacity. The combined collector could increase the scheelite recovery by 3.48% at low temperatures of 8–12℃. This is particularly relevant in the Luanchuan area, which has the largest scheelite concentrate output in China. The results confirmed that ODB enhanced the collection capability of NaOl by improving the dispersion and foaming performance. Betaine can be introduced as an additive to NaOl to improve the recovery of scheelite at low temperatures.展开更多
Mobile Edge Computing(MEC)is a technology designed for the on-demand provisioning of computing and storage services,strategically positioned close to users.In the MEC environment,frequently accessed content can be dep...Mobile Edge Computing(MEC)is a technology designed for the on-demand provisioning of computing and storage services,strategically positioned close to users.In the MEC environment,frequently accessed content can be deployed and cached on edge servers to optimize the efficiency of content delivery,ultimately enhancing the quality of the user experience.However,due to the typical placement of edge devices and nodes at the network’s periphery,these components may face various potential fault tolerance challenges,including network instability,device failures,and resource constraints.Considering the dynamic nature ofMEC,making high-quality content caching decisions for real-time mobile applications,especially those sensitive to latency,by effectively utilizing mobility information,continues to be a significant challenge.In response to this challenge,this paper introduces FT-MAACC,a mobility-aware caching solution grounded in multi-agent deep reinforcement learning and equipped with fault tolerance mechanisms.This approach comprehensively integrates content adaptivity algorithms to evaluate the priority of highly user-adaptive cached content.Furthermore,it relies on collaborative caching strategies based onmulti-agent deep reinforcement learningmodels and establishes a fault-tolerancemodel to ensure the system’s reliability,availability,and persistence.Empirical results unequivocally demonstrate that FTMAACC outperforms its peer methods in cache hit rates and transmission latency.展开更多
Accurately picking P-and S-wave arrivals of microseismic(MS)signals in real-time directly influences the early warning of rock mass failure.A common contradiction between accuracy and computation exists in the current...Accurately picking P-and S-wave arrivals of microseismic(MS)signals in real-time directly influences the early warning of rock mass failure.A common contradiction between accuracy and computation exists in the current arrival picking methods.Thus,a real-time arrival picking method of MS signals is constructed based on a convolutional-recurrent neural network(CRNN).This method fully utilizes the advantages of convolutional layers and gated recurrent units(GRU)in extracting short-and long-term features,in order to create a precise and lightweight arrival picking structure.Then,the synthetic signals with field noises are used to evaluate the hyperparameters of the CRNN model and obtain an optimal CRNN model.The actual operation on various devices indicates that compared with the U-Net method,the CRNN method achieves faster arrival picking with less performance consumption.An application of large underground caverns in the Yebatan hydropower station(YBT)project shows that compared with the short-term average/long-term average(STA/LTA),Akaike information criterion(AIC)and U-Net methods,the CRNN method has the highest accuracy within four sampling points,which is 87.44%for P-wave and 91.29%for S-wave,respectively.The sum of mean absolute errors(MAESUM)of the CRNN method is 4.22 sampling points,which is lower than that of the other methods.Among the four methods,the MS sources location calculated based on the CRNN method shows the best consistency with the actual failure,which occurs at the junction of the shaft and the second gallery.Thus,the proposed method can pick up P-and S-arrival accurately and rapidly,providing a reference for rock failure analysis and evaluation in engineering applications.展开更多
As a typical nonlinear wave,forward-leaning waves can be frequently encountered in the near-shore areas,which can impact coastal sediment transport significantly.Hence,it is of significance to describe the characteris...As a typical nonlinear wave,forward-leaning waves can be frequently encountered in the near-shore areas,which can impact coastal sediment transport significantly.Hence,it is of significance to describe the characteristics of the boundary layer beneath forward-leaning waves accurately,especially for the turbulent boundary layer.In this work,the linearized turbulent boundary layer model with a linear turbulent viscosity coefficient is applied,and the novel expression of the near-bed orbital velocity that has been worked out by the authors for forward-leaning waves of arbitrary forward-leaning degrees is further used to specify the free stream boundary condition of the bottom boundary layer.Then,a variable transformation is found so as to make the equation of the turbulent boundary layer model be solved analytically through a modified Bessel function.Consequently,an explicit analytical solution of the turbulent boundary layer beneath forward-leaning waves is derived by means of variable separation and variable transformation.The analytical solutions of the velocity profile and bottom shear stress of the turbulent boundary layer beneath forward-leaning waves are verified by comparing the present analytical results with typical experimental data available in the previous literature.展开更多
In this study, a three dimensional(3D) numerical model of six-degrees-of-freedom(6DOF) is applied to simulate the water entries of twin spheres side-by-side at different lateral distances and time intervals.The turbul...In this study, a three dimensional(3D) numerical model of six-degrees-of-freedom(6DOF) is applied to simulate the water entries of twin spheres side-by-side at different lateral distances and time intervals.The turbulence structure is described using the shear-stress transport k-ω(SST k-ω) model, and the volume of fluid(VOF) method is used to track the complex air-liquid interface. The motion of spheres during water entry is simulated using an independent overset grid. The numerical model is verified by comparing the cavity evolution results from simulations and experiments. Numerical results reveal that the time interval between the twin water entries evidently affects cavity expansion and contraction behaviors in the radial direction. However, this influence is significantly weakened by increasing the lateral distance between the two spheres. In synchronous water entries, pressure is reduced on the midline of two cavities during surface closure, which is directly related to the cavity volume. The evolution of vortexes inside the two cavities is analyzed using a velocity vector field, which is affected by the lateral distance and time interval of water entries.展开更多
BACKGROUND Ulcerative colitis is a chronic inflammatory disease of the colon with an unknown etiology.Alkaline sphingomyelinase(alk-SMase)is specifically expressed by intestinal epithelial cells,and has been reported ...BACKGROUND Ulcerative colitis is a chronic inflammatory disease of the colon with an unknown etiology.Alkaline sphingomyelinase(alk-SMase)is specifically expressed by intestinal epithelial cells,and has been reported to play an anti-inflammatory role.However,the underlying mechanism is still unclear.AIM To explore the mechanism of alk-SMase anti-inflammatory effects on intestinal barrier function and oxidative stress in dextran sulfate sodium(DSS)-induced colitis.METHODS Mice were administered 3%DSS drinking water,and disease activity index was determined to evaluate the status of colitis.Intestinal permeability was evaluated by gavage administration of fluorescein isothiocyanate dextran,and bacterial translocation was evaluated by measuring serum lipopolysaccharide.Intestinal epithelial cell ultrastructure was observed by electron microscopy.Western blotting and quantitative real-time reverse transcription-polymerase chain reaction were used to detect the expression of intestinal barrier proteins and mRNA,respectively.Serum oxidant and antioxidant marker levels were analyzed using commercial kits to assess oxidative stress levels.RESULTS Compared to wild-type(WT)mice,inflammation and intestinal permeability in alk-SMase knockout(KO)mice were more severe beginning 4 d after DSS induction.The mRNA and protein levels of intestinal barrier proteins,including zonula occludens-1,occludin,claudin-3,claudin-5,claudin-8,mucin 2,and secretory immunoglobulin A,were significantly reduced on 4 d after DSS treatment.Ultrastructural observations revealed progressive damage to the tight junctions of intestinal epithelial cells.Furthermore,by day 4,mitochondria appeared swollen and degenerated.Additionally,compared to WT mice,serum malondialdehyde levels in KO mice were higher,and the antioxidant capacity was significantly lower.The expression of the transcription factor nuclear factor erythroid 2-related factor 2(Nrf2)in the colonic mucosal tissue of KO mice was significantly decreased after DSS treatment.mRNA levels of Nrf2-regulated downstream antioxidant enzymes were also decreased.Finally,colitis in KO mice could be effectively relieved by the injection of tertiary butylhydroquinone,which is an Nrf2 activator.CONCLUSION Alk-SMase regulates the stability of the intestinal mucosal barrier and enhances antioxidant activity through the Nrf2 signaling pathway.展开更多
Pulse rate is one of the important characteristics of traditional Chinese medicine pulse diagnosis,and it is of great significance for determining the nature of cold and heat in diseases.The prediction of pulse rate b...Pulse rate is one of the important characteristics of traditional Chinese medicine pulse diagnosis,and it is of great significance for determining the nature of cold and heat in diseases.The prediction of pulse rate based on facial video is an exciting research field for getting palpation information by observation diagnosis.However,most studies focus on optimizing the algorithm based on a small sample of participants without systematically investigating multiple influencing factors.A total of 209 participants and 2,435 facial videos,based on our self-constructed Multi-Scene Sign Dataset and the public datasets,were used to perform a multi-level and multi-factor comprehensive comparison.The effects of different datasets,blood volume pulse signal extraction algorithms,region of interests,time windows,color spaces,pulse rate calculation methods,and video recording scenes were analyzed.Furthermore,we proposed a blood volume pulse signal quality optimization strategy based on the inverse Fourier transform and an improvement strategy for pulse rate estimation based on signal-to-noise ratio threshold sliding.We found that the effects of video estimation of pulse rate in the Multi-Scene Sign Dataset and Pulse Rate Detection Dataset were better than in other datasets.Compared with Fast independent component analysis and Single Channel algorithms,chrominance-based method and plane-orthogonal-to-skin algorithms have a more vital anti-interference ability and higher robustness.The performances of the five-organs fusion area and the full-face area were better than that of single sub-regions,and the fewer motion artifacts and better lighting can improve the precision of pulse rate estimation.展开更多
Objective: To investigate the expression of Platelet-derived growth factor receptor alpha (PDGFR-α) in patients who have valvular atrial fibrillation. Methods: In this research, eighty-four patients with rheumatic he...Objective: To investigate the expression of Platelet-derived growth factor receptor alpha (PDGFR-α) in patients who have valvular atrial fibrillation. Methods: In this research, eighty-four patients with rheumatic heart disease who were going to undertake cardiac surgery were included. The subjects were divided into two groups: the AF group and the sinus rhythm group, the quantities are 39 and 45 respectively. Before the surgery, baseline demographics, physical examination, routine laboratory testing, echocardiography, echocardiographic data and additional clinical data were available for all patients. The right atrial tissue of the subjects was separated during surgery, with an area of approximately 0.3 - 0.5 mm<sup>3</sup>. Immunofluorescence staining was used to analyze the distribution of PDGFR-α of atrial tissue. mRNA of PDGFR-α in atrial tissue were determined by real-time quantitative PCR (Polymerase Chain Reaction);Western-Blot technique was used to measure the protein of PDGFR-α in atrial tissue. Results: There were no significant differences (P > 0.05) in sex ratio, age, blood pressure, blood biochemistry, and other aspects of medical history between the two groups. However, the right and left atrium diameters in the AF group were markedly larger than those in the SR group (P α from right atrial tissue were obviously higher in the AF group than that in the SR group (P Conclusion: The expression of PDGFR-α in the right atrial tissue of patients with atrial fibrillation was found to be significantly higher.展开更多
Objective:To evaluate the safety and effectiveness of Xuefu Zhuyu Decoction in treating diabetic encephalopathy.Methods:Computer search and manual search of Pub Med,Web of Science,The Cochrane Library,CNKI,WF,VIP and ...Objective:To evaluate the safety and effectiveness of Xuefu Zhuyu Decoction in treating diabetic encephalopathy.Methods:Computer search and manual search of Pub Med,Web of Science,The Cochrane Library,CNKI,WF,VIP and CBM from January 2000 to April 2020 on the treatment of diabetic encephalopathy with Xuefu Zhuyu Decoction In randomized controlled clinical trials,select documents that meet the criteria for analysis using Rev Man 5.3 software.Results:A total of 11 articles and 838 patients were included in the study.Metaanalysis showed that Xuefu Zhuyu Decoction was effective(OR=3.71,95%CI[2.42,5.70],P<0.00001),and the patient’s neurological deficit score(MD=-4.52,95%CI[-5.10,-3.94],P<0.00001),fasting blood glucose(MD=-1.05,95%CI[-1.51,-0.59],P<0.00001),2 hours postprandial blood glucose(MD=-1.31,95%CI[-1.94,-0.69],P<0.00001),glycated hemoglobin(MD=-0.94,95%CI[-1.15,-0.72],P<0.00001),TCM symptom score(MD=-2.44,95%CI[-3.82,-1.06],P=0.0005<0.01),vitality score(MD=12.79,95%CI[8.79,16.79],P<0.00001),the improvement effect is obvious Better than conventional Western medicine treatment.Conclusion:Xuefu Zhuyu Decoction can improve the clinical efficiency of patients with diabetic encephalopathy,reduce brain damage,reduce blood sugar and glycated hemoglobin levels,and has good safety.However,the quality of some of the included literatures is low,and the number of clinical cases is small,which may affect the authenticity of the Meta analysis results.Still need to be further verified by clinical randomized controlled trials with high-quality large samples.展开更多
Grapevine is one of the most economically important crops worldwide.However,the previous versions of the grapevine reference genome tipically consist of thousands of fragments with missing centromeres and telomeres,li...Grapevine is one of the most economically important crops worldwide.However,the previous versions of the grapevine reference genome tipically consist of thousands of fragments with missing centromeres and telomeres,limiting the accessibility of the repetitive sequences,the centromeric and telomeric regions,and the study of inheritance of important agronomic traits in these regions.Here,we assembled a telomere-to-telomere(T2T)gap-free reference genome for the cultivar PN40024 using PacBio HiFi long reads.The T2T reference genome(PN_T2T)is 69 Mb longer with 9018 more genes identified than the 12X.v0 version.We annotated 67%repetitive sequences,19 centromeres and 36 telomeres,and incorporated gene annotations of previous versions into the PN_T2T assembly.We detected a total of 377 gene clusters,which showed associations with complex traits,such as aroma and disease resistance.Even though PN40024 derives from nine generations of selfing,we still found nine genomic hotspots of heterozygous sites associated with biological processes,such as the oxidation–reduction process and protein phosphorylation.The fully annotated complete reference genome therefore constitutes an important resource for grapevine genetic studies and breeding programs.展开更多
Kiwifruit is an economically and nutritionally important fruit crop with extremely high contents of vitamin C.However,the previously released versions of kiwifruit genomes all have a mass of unanchored or missing regi...Kiwifruit is an economically and nutritionally important fruit crop with extremely high contents of vitamin C.However,the previously released versions of kiwifruit genomes all have a mass of unanchored or missing regions.Here,we report a highly continuous and completely gap-free reference genome of Actinidia chinensis cv.‘Hongyang’,named Hongyang v4.0,which is the first to achieve two de novo haploid-resolved haplotypes,HY4P and HY4A.HY4P and HY4A have a total length of 606.1 and 599.6 Mb,respectively,with almost the entire telomeres and centromeres assembled in each haplotype.In comparison with Hongyang v3.0,the integrity and contiguity of Hongyang v4.0 is markedly improved by filling all unclosed gaps and correcting some misoriented regions,resulting in∼38.6–39.5 Mb extra sequences,which might affect 4263 and 4244 protein-coding genes in HY4P and HY4A,respectively.Furthermore,our gap-free genome assembly provides the first clue for inspecting the structure and function of centromeres.Globally,centromeric regions are characterized by higher-order repeats that mainly consist of a 153-bp conserved centromere-specific monomer(Ach-CEN153)with different copy numbers among chromosomes.Functional enrichment analysis of the genes located within centromeric regions demonstrates that chromosome centromeres may not only play physical roles for linking a pair of sister chromatids,but also have genetic features for participation in the regulation of cell division.The availability of the telomere-to-telomere and gap-free Hongyang v4.0 reference genome lays a solid foundation not only for illustrating genome structure and functional genomics studies but also for facilitating kiwifruit breeding and improvement.展开更多
A high-quality genome is the basis for studies on functional,evolutionary,and comparative genomics.The majority of attention has been paid to the solution of complex chromosome structures and highly repetitive sequenc...A high-quality genome is the basis for studies on functional,evolutionary,and comparative genomics.The majority of attention has been paid to the solution of complex chromosome structures and highly repetitive sequences,along with the emergence of a new‘telomere-to-telomere(T2T)assembly’era.However,the bioinformatic tools for the automatic construction and/or characterization of T2T genome are limited.Here,we developed a user-friendly web toolkit,quarTeT,which currently includes four modules:AssemblyMapper,GapFiller,TeloExplorer,and CentroMiner.First,AssemblyMapper is designed to assemble phased contigs into the chromosome-level genome by referring to a closely related genome.Then,GapFiller would endeavor to fill all unclosed gaps in a given genome with the aid of additional ultra-long sequences.Finally,TeloExplorer and CentroMiner are applied to identify candidate telomere and centromere as well as their localizations on each chromosome.These four modules can be used alone or in combination with each other for T2T genome assembly and characterization.As a case study,by adopting the entire modular functions of quarTeT,we have achieved the Actinidia chinensis genome assembly that is of a quality comparable to the reported genome Hongyang v4.0,which was assembled with the addition of manual handling.Further evaluation of CentroMiner by searching centromeres in Arabidopsis thaliana and Oryza sativa genomes showed that quarTeT is capable of identifying all the centromeric regions that have been previously detected by experimental methods.Collectively,quarTeT is an efficient toolkit for studies of large-scale T2T genomes and can be accessed at http://www.atcgn.com:8080/quarTeT/home.html without registration.展开更多
基金financially supported by the National Natural Science Foundation of China (Nos.51904339 and No.51974364)the Key Laboratory of Hunan Province for Clean and Efficient Utilization of Strategic Calcium-containing Mineral Resources,China (No.2018TP1002)the Co-Innovation Centre for Clean and Efficient Utilization of Strategic Metal Mineral Resources,and the Postgraduate Independent Exploration and Innovation Project of Central South University,China (No.2018zzts224)。
文摘The impact of alkyl dimethyl betaine (ADB) on the collection capacity of sodium oleate (NaOl) at low temperatures was evaluated using flotation tests at various scales. The low-temperature synergistic mechanism of ADB and NaOl was explored by infrared spectroscopy, X-ray photoelectron spectroscopy, surface tension measurement, foam performance test, and flotation reagent size measurement.The flotation tests revealed that the collector mixed with octadecyl dimethyl betaine (ODB) and NaOl in a mass ratio of 4:96 exhibited the highest collection capacity. The combined collector could increase the scheelite recovery by 3.48% at low temperatures of 8–12℃. This is particularly relevant in the Luanchuan area, which has the largest scheelite concentrate output in China. The results confirmed that ODB enhanced the collection capability of NaOl by improving the dispersion and foaming performance. Betaine can be introduced as an additive to NaOl to improve the recovery of scheelite at low temperatures.
基金supported by the Innovation Fund Project of Jiangxi Normal University(YJS2022065)the Domestic Visiting Program of Jiangxi Normal University.
文摘Mobile Edge Computing(MEC)is a technology designed for the on-demand provisioning of computing and storage services,strategically positioned close to users.In the MEC environment,frequently accessed content can be deployed and cached on edge servers to optimize the efficiency of content delivery,ultimately enhancing the quality of the user experience.However,due to the typical placement of edge devices and nodes at the network’s periphery,these components may face various potential fault tolerance challenges,including network instability,device failures,and resource constraints.Considering the dynamic nature ofMEC,making high-quality content caching decisions for real-time mobile applications,especially those sensitive to latency,by effectively utilizing mobility information,continues to be a significant challenge.In response to this challenge,this paper introduces FT-MAACC,a mobility-aware caching solution grounded in multi-agent deep reinforcement learning and equipped with fault tolerance mechanisms.This approach comprehensively integrates content adaptivity algorithms to evaluate the priority of highly user-adaptive cached content.Furthermore,it relies on collaborative caching strategies based onmulti-agent deep reinforcement learningmodels and establishes a fault-tolerancemodel to ensure the system’s reliability,availability,and persistence.Empirical results unequivocally demonstrate that FTMAACC outperforms its peer methods in cache hit rates and transmission latency.
基金We acknowledge the funding support from National Natural Science Foundation of China(Grant No.42077263).
文摘Accurately picking P-and S-wave arrivals of microseismic(MS)signals in real-time directly influences the early warning of rock mass failure.A common contradiction between accuracy and computation exists in the current arrival picking methods.Thus,a real-time arrival picking method of MS signals is constructed based on a convolutional-recurrent neural network(CRNN).This method fully utilizes the advantages of convolutional layers and gated recurrent units(GRU)in extracting short-and long-term features,in order to create a precise and lightweight arrival picking structure.Then,the synthetic signals with field noises are used to evaluate the hyperparameters of the CRNN model and obtain an optimal CRNN model.The actual operation on various devices indicates that compared with the U-Net method,the CRNN method achieves faster arrival picking with less performance consumption.An application of large underground caverns in the Yebatan hydropower station(YBT)project shows that compared with the short-term average/long-term average(STA/LTA),Akaike information criterion(AIC)and U-Net methods,the CRNN method has the highest accuracy within four sampling points,which is 87.44%for P-wave and 91.29%for S-wave,respectively.The sum of mean absolute errors(MAESUM)of the CRNN method is 4.22 sampling points,which is lower than that of the other methods.Among the four methods,the MS sources location calculated based on the CRNN method shows the best consistency with the actual failure,which occurs at the junction of the shaft and the second gallery.Thus,the proposed method can pick up P-and S-arrival accurately and rapidly,providing a reference for rock failure analysis and evaluation in engineering applications.
基金Project supported by the National Key R&D Program of China(No.2022YFC3204303)the National Natural Science Foundation of China(Nos.12202503,12132018,and 52394254)。
文摘As a typical nonlinear wave,forward-leaning waves can be frequently encountered in the near-shore areas,which can impact coastal sediment transport significantly.Hence,it is of significance to describe the characteristics of the boundary layer beneath forward-leaning waves accurately,especially for the turbulent boundary layer.In this work,the linearized turbulent boundary layer model with a linear turbulent viscosity coefficient is applied,and the novel expression of the near-bed orbital velocity that has been worked out by the authors for forward-leaning waves of arbitrary forward-leaning degrees is further used to specify the free stream boundary condition of the bottom boundary layer.Then,a variable transformation is found so as to make the equation of the turbulent boundary layer model be solved analytically through a modified Bessel function.Consequently,an explicit analytical solution of the turbulent boundary layer beneath forward-leaning waves is derived by means of variable separation and variable transformation.The analytical solutions of the velocity profile and bottom shear stress of the turbulent boundary layer beneath forward-leaning waves are verified by comparing the present analytical results with typical experimental data available in the previous literature.
基金China Academy of Launch Vehicle Technology(Grant No.CALT-2022-03)Science and Technology on Underwater Information and Control Laboratory(Grant No.2021-JCJQ-LB-030-05).
文摘In this study, a three dimensional(3D) numerical model of six-degrees-of-freedom(6DOF) is applied to simulate the water entries of twin spheres side-by-side at different lateral distances and time intervals.The turbulence structure is described using the shear-stress transport k-ω(SST k-ω) model, and the volume of fluid(VOF) method is used to track the complex air-liquid interface. The motion of spheres during water entry is simulated using an independent overset grid. The numerical model is verified by comparing the cavity evolution results from simulations and experiments. Numerical results reveal that the time interval between the twin water entries evidently affects cavity expansion and contraction behaviors in the radial direction. However, this influence is significantly weakened by increasing the lateral distance between the two spheres. In synchronous water entries, pressure is reduced on the midline of two cavities during surface closure, which is directly related to the cavity volume. The evolution of vortexes inside the two cavities is analyzed using a velocity vector field, which is affected by the lateral distance and time interval of water entries.
基金the Natural Science Foundation of Hainan Province,No.823MS046the Talent Program of Hainan Medical University,No.XRC2022007.
文摘BACKGROUND Ulcerative colitis is a chronic inflammatory disease of the colon with an unknown etiology.Alkaline sphingomyelinase(alk-SMase)is specifically expressed by intestinal epithelial cells,and has been reported to play an anti-inflammatory role.However,the underlying mechanism is still unclear.AIM To explore the mechanism of alk-SMase anti-inflammatory effects on intestinal barrier function and oxidative stress in dextran sulfate sodium(DSS)-induced colitis.METHODS Mice were administered 3%DSS drinking water,and disease activity index was determined to evaluate the status of colitis.Intestinal permeability was evaluated by gavage administration of fluorescein isothiocyanate dextran,and bacterial translocation was evaluated by measuring serum lipopolysaccharide.Intestinal epithelial cell ultrastructure was observed by electron microscopy.Western blotting and quantitative real-time reverse transcription-polymerase chain reaction were used to detect the expression of intestinal barrier proteins and mRNA,respectively.Serum oxidant and antioxidant marker levels were analyzed using commercial kits to assess oxidative stress levels.RESULTS Compared to wild-type(WT)mice,inflammation and intestinal permeability in alk-SMase knockout(KO)mice were more severe beginning 4 d after DSS induction.The mRNA and protein levels of intestinal barrier proteins,including zonula occludens-1,occludin,claudin-3,claudin-5,claudin-8,mucin 2,and secretory immunoglobulin A,were significantly reduced on 4 d after DSS treatment.Ultrastructural observations revealed progressive damage to the tight junctions of intestinal epithelial cells.Furthermore,by day 4,mitochondria appeared swollen and degenerated.Additionally,compared to WT mice,serum malondialdehyde levels in KO mice were higher,and the antioxidant capacity was significantly lower.The expression of the transcription factor nuclear factor erythroid 2-related factor 2(Nrf2)in the colonic mucosal tissue of KO mice was significantly decreased after DSS treatment.mRNA levels of Nrf2-regulated downstream antioxidant enzymes were also decreased.Finally,colitis in KO mice could be effectively relieved by the injection of tertiary butylhydroquinone,which is an Nrf2 activator.CONCLUSION Alk-SMase regulates the stability of the intestinal mucosal barrier and enhances antioxidant activity through the Nrf2 signaling pathway.
基金supported by the Key Research Program of the Chinese Academy of Sciences(grant number ZDRW-ZS-2021-1-2).
文摘Pulse rate is one of the important characteristics of traditional Chinese medicine pulse diagnosis,and it is of great significance for determining the nature of cold and heat in diseases.The prediction of pulse rate based on facial video is an exciting research field for getting palpation information by observation diagnosis.However,most studies focus on optimizing the algorithm based on a small sample of participants without systematically investigating multiple influencing factors.A total of 209 participants and 2,435 facial videos,based on our self-constructed Multi-Scene Sign Dataset and the public datasets,were used to perform a multi-level and multi-factor comprehensive comparison.The effects of different datasets,blood volume pulse signal extraction algorithms,region of interests,time windows,color spaces,pulse rate calculation methods,and video recording scenes were analyzed.Furthermore,we proposed a blood volume pulse signal quality optimization strategy based on the inverse Fourier transform and an improvement strategy for pulse rate estimation based on signal-to-noise ratio threshold sliding.We found that the effects of video estimation of pulse rate in the Multi-Scene Sign Dataset and Pulse Rate Detection Dataset were better than in other datasets.Compared with Fast independent component analysis and Single Channel algorithms,chrominance-based method and plane-orthogonal-to-skin algorithms have a more vital anti-interference ability and higher robustness.The performances of the five-organs fusion area and the full-face area were better than that of single sub-regions,and the fewer motion artifacts and better lighting can improve the precision of pulse rate estimation.
文摘Objective: To investigate the expression of Platelet-derived growth factor receptor alpha (PDGFR-α) in patients who have valvular atrial fibrillation. Methods: In this research, eighty-four patients with rheumatic heart disease who were going to undertake cardiac surgery were included. The subjects were divided into two groups: the AF group and the sinus rhythm group, the quantities are 39 and 45 respectively. Before the surgery, baseline demographics, physical examination, routine laboratory testing, echocardiography, echocardiographic data and additional clinical data were available for all patients. The right atrial tissue of the subjects was separated during surgery, with an area of approximately 0.3 - 0.5 mm<sup>3</sup>. Immunofluorescence staining was used to analyze the distribution of PDGFR-α of atrial tissue. mRNA of PDGFR-α in atrial tissue were determined by real-time quantitative PCR (Polymerase Chain Reaction);Western-Blot technique was used to measure the protein of PDGFR-α in atrial tissue. Results: There were no significant differences (P > 0.05) in sex ratio, age, blood pressure, blood biochemistry, and other aspects of medical history between the two groups. However, the right and left atrium diameters in the AF group were markedly larger than those in the SR group (P α from right atrial tissue were obviously higher in the AF group than that in the SR group (P Conclusion: The expression of PDGFR-α in the right atrial tissue of patients with atrial fibrillation was found to be significantly higher.
基金supported by the National Natural Science Foundation of China(81973690 and 81904049)Experimental Technology Standardization Research Project of Beijing University of Chinese Medicine(2021-SYJS-001).
基金National natural science foundation of China(No.81973796)。
文摘Objective:To evaluate the safety and effectiveness of Xuefu Zhuyu Decoction in treating diabetic encephalopathy.Methods:Computer search and manual search of Pub Med,Web of Science,The Cochrane Library,CNKI,WF,VIP and CBM from January 2000 to April 2020 on the treatment of diabetic encephalopathy with Xuefu Zhuyu Decoction In randomized controlled clinical trials,select documents that meet the criteria for analysis using Rev Man 5.3 software.Results:A total of 11 articles and 838 patients were included in the study.Metaanalysis showed that Xuefu Zhuyu Decoction was effective(OR=3.71,95%CI[2.42,5.70],P<0.00001),and the patient’s neurological deficit score(MD=-4.52,95%CI[-5.10,-3.94],P<0.00001),fasting blood glucose(MD=-1.05,95%CI[-1.51,-0.59],P<0.00001),2 hours postprandial blood glucose(MD=-1.31,95%CI[-1.94,-0.69],P<0.00001),glycated hemoglobin(MD=-0.94,95%CI[-1.15,-0.72],P<0.00001),TCM symptom score(MD=-2.44,95%CI[-3.82,-1.06],P=0.0005<0.01),vitality score(MD=12.79,95%CI[8.79,16.79],P<0.00001),the improvement effect is obvious Better than conventional Western medicine treatment.Conclusion:Xuefu Zhuyu Decoction can improve the clinical efficiency of patients with diabetic encephalopathy,reduce brain damage,reduce blood sugar and glycated hemoglobin levels,and has good safety.However,the quality of some of the included literatures is low,and the number of clinical cases is small,which may affect the authenticity of the Meta analysis results.Still need to be further verified by clinical randomized controlled trials with high-quality large samples.
基金This work was supported by the National Natural Science Fund for Excellent Young Scientists Fund Program(Overseas)to Y.Z.,the National Key Research and Development Program of China(grant 2019YFA0906200)the Agricultural Science and Technology Innovation Program(CAAS-ZDRW202101)+1 种基金the Shenzhen Science and Technology Program(grant KQTD2016113010482651)the BMBF-funded de.
文摘Grapevine is one of the most economically important crops worldwide.However,the previous versions of the grapevine reference genome tipically consist of thousands of fragments with missing centromeres and telomeres,limiting the accessibility of the repetitive sequences,the centromeric and telomeric regions,and the study of inheritance of important agronomic traits in these regions.Here,we assembled a telomere-to-telomere(T2T)gap-free reference genome for the cultivar PN40024 using PacBio HiFi long reads.The T2T reference genome(PN_T2T)is 69 Mb longer with 9018 more genes identified than the 12X.v0 version.We annotated 67%repetitive sequences,19 centromeres and 36 telomeres,and incorporated gene annotations of previous versions into the PN_T2T assembly.We detected a total of 377 gene clusters,which showed associations with complex traits,such as aroma and disease resistance.Even though PN40024 derives from nine generations of selfing,we still found nine genomic hotspots of heterozygous sites associated with biological processes,such as the oxidation–reduction process and protein phosphorylation.The fully annotated complete reference genome therefore constitutes an important resource for grapevine genetic studies and breeding programs.
基金supported by funds from the National Natural Science Foundation of China(31972474,90717110)Hubei Natural Science Fund for Distinguished Young Scholars(2020CFA062).
文摘Kiwifruit is an economically and nutritionally important fruit crop with extremely high contents of vitamin C.However,the previously released versions of kiwifruit genomes all have a mass of unanchored or missing regions.Here,we report a highly continuous and completely gap-free reference genome of Actinidia chinensis cv.‘Hongyang’,named Hongyang v4.0,which is the first to achieve two de novo haploid-resolved haplotypes,HY4P and HY4A.HY4P and HY4A have a total length of 606.1 and 599.6 Mb,respectively,with almost the entire telomeres and centromeres assembled in each haplotype.In comparison with Hongyang v3.0,the integrity and contiguity of Hongyang v4.0 is markedly improved by filling all unclosed gaps and correcting some misoriented regions,resulting in∼38.6–39.5 Mb extra sequences,which might affect 4263 and 4244 protein-coding genes in HY4P and HY4A,respectively.Furthermore,our gap-free genome assembly provides the first clue for inspecting the structure and function of centromeres.Globally,centromeric regions are characterized by higher-order repeats that mainly consist of a 153-bp conserved centromere-specific monomer(Ach-CEN153)with different copy numbers among chromosomes.Functional enrichment analysis of the genes located within centromeric regions demonstrates that chromosome centromeres may not only play physical roles for linking a pair of sister chromatids,but also have genetic features for participation in the regulation of cell division.The availability of the telomere-to-telomere and gap-free Hongyang v4.0 reference genome lays a solid foundation not only for illustrating genome structure and functional genomics studies but also for facilitating kiwifruit breeding and improvement.
基金This work was supported by funds from the National Natural Science Foundation of China(31972474,31471157)the Open Fund of State Key Laboratory of Tea Plant Biology and Utilization(SKLTOF20150103).
文摘A high-quality genome is the basis for studies on functional,evolutionary,and comparative genomics.The majority of attention has been paid to the solution of complex chromosome structures and highly repetitive sequences,along with the emergence of a new‘telomere-to-telomere(T2T)assembly’era.However,the bioinformatic tools for the automatic construction and/or characterization of T2T genome are limited.Here,we developed a user-friendly web toolkit,quarTeT,which currently includes four modules:AssemblyMapper,GapFiller,TeloExplorer,and CentroMiner.First,AssemblyMapper is designed to assemble phased contigs into the chromosome-level genome by referring to a closely related genome.Then,GapFiller would endeavor to fill all unclosed gaps in a given genome with the aid of additional ultra-long sequences.Finally,TeloExplorer and CentroMiner are applied to identify candidate telomere and centromere as well as their localizations on each chromosome.These four modules can be used alone or in combination with each other for T2T genome assembly and characterization.As a case study,by adopting the entire modular functions of quarTeT,we have achieved the Actinidia chinensis genome assembly that is of a quality comparable to the reported genome Hongyang v4.0,which was assembled with the addition of manual handling.Further evaluation of CentroMiner by searching centromeres in Arabidopsis thaliana and Oryza sativa genomes showed that quarTeT is capable of identifying all the centromeric regions that have been previously detected by experimental methods.Collectively,quarTeT is an efficient toolkit for studies of large-scale T2T genomes and can be accessed at http://www.atcgn.com:8080/quarTeT/home.html without registration.
