This study aimed to investigate the dose-effect of iron on growth performance,antioxidant function.intestinal morphology,and mRNA expression of jejunal tight junction protein in 1-to21-d-old yellow-feathered broilers....This study aimed to investigate the dose-effect of iron on growth performance,antioxidant function.intestinal morphology,and mRNA expression of jejunal tight junction protein in 1-to21-d-old yellow-feathered broilers.A total of 7201-d-old yellow-feathered maleb roilers were allocated to 9 treatments with 8 replicate cages of 10 birds per cage.The dietary treatments were consisted of a basal diet(contained 79.6 mg Fe kg^(-1))supplemented with 0,20,40,60,80,160,320,640,and 1,280 mg Fe kg^(-1)in the form of FeSO_(4)·7H_(2)O.Compared with the birds in the control group,birds supplemented with 20mg Fe kg^(-1)had higher average daily gain(ADG)(P<0.0001).Adding 640 and 1,280 mg Fe kg^(-1)significantly decreased ADG(P<0.0001)and average daily feed intake(ADFI)(P<0.0001)compared with supplementation of 20mg Fe kg^(-1).Malondialdehyde(MDA)concentration in plasma and duodenum increased linearly(P<0.0001),but MDA concentration in liver and jejunum increased linearly(P<0.05)or quadratically(P<0.05)with increased dietary Fe concentration.The villus height(VH)in duodenum and jejunum,and the ratio of villus height to crypt depth(V/C)in duodenum decreased linearly(P?0.05)as dietary Feincreased.As dietary Fe increased,the jejunal relative mRNA abundance of claudin-1 decreased linearly(P=0.001),but the jejunal relative mRNA abundance of zona occludens-1(ZO-1)and occludin decreased linearly(P?0.05)or quadratically(P?0.05).Compared with the supplementation of 20 mg Fe kg^(-1),the supplementation of640 mg Fe kg^(-1)or higher increased(P?0.05)MDA concentrations in plasma,duodenum,and jejunum,decreased VH in the duodenum and jejunum,and the addition of 1,280 mg Fe kg^(-1)reduced(P?0.05)the jejunal tight junction protein(claudin-1,ZO-1,occludin)mRNA abundance.In summary,640 mg of supplemental Fe kg^(-1)or greater was associated with decreased growth performance,increased oxidative stress,disrupted intestinal morphology,and reduced mRNA expression of jejunal tight junction protein.展开更多
The gut microbiota plays a pivotal role in the immunomodulatory and protumorigenic microenvironment of colorectal cancer(CRC).However,the effect of ginsenoside Rk3(Rk3)on CRC and gut microbiota remains unclear.Therefo...The gut microbiota plays a pivotal role in the immunomodulatory and protumorigenic microenvironment of colorectal cancer(CRC).However,the effect of ginsenoside Rk3(Rk3)on CRC and gut microbiota remains unclear.Therefore,the purpose of this study is to explore the potential effect of Rk3 on CRC from the perspective of gut microbiota and immune regulation.Our results reveal that treatment with Rk3 significantly suppresses the formation of colon tumors,repairs intestinal barrier damage,and regulates the gut microbiota imbalance caused by CRC,including enrichment of probiotics such as Akkermansia muciniphila and Barnesiella intestinihominis,and clearance of pathogenic Desulfovibrio.Subsequent metabolomics data demonstrate that Rk3 can modulate the metabolism of amino acids and bile acids,particularly by upregulating glutamine,which has the potential to regulate the immune response.Furthermore,we elucidate the regulatory effects of Rk3 on chemokines and inflammatory factors associated with group 3 innate lymphoid cells(ILC3s)and T helper 17(Th17)signaling pathways,which inhibits the hyperactivation of the Janus kinase-signal transducer and activator of transcription 3(JAK-STAT3)signaling pathway.These results indicate that Rk3 modulates gut microbiota,regulates ILC3s immune response,and inhibits the JAK-STAT3 signaling pathway to suppress the development of colon tumors.More importantly,the results of fecal microbiota transplantation suggest that the inhibitory effect of Rk3 on colon tumors and its regulation of ILC3 immune responses are mediated by the gut microbiota.In summary,these findings emphasize that Rk3 can be utilized as a regulator of the gut microbiota for the prevention and treatment of CRC.展开更多
In-situ upgrading by heating is feasible for low-maturity shale oil,where the pore space dynamically evolves.We characterize this response for a heated substrate concurrently imaged by SEM.We systematically follow the...In-situ upgrading by heating is feasible for low-maturity shale oil,where the pore space dynamically evolves.We characterize this response for a heated substrate concurrently imaged by SEM.We systematically follow the evolution of pore quantity,size(length,width and cross-sectional area),orientation,shape(aspect ratio,roundness and solidity)and their anisotropy—interpreted by machine learning.Results indicate that heating generates new pores in both organic matter and inorganic minerals.However,the newly formed pores are smaller than the original pores and thus reduce average lengths and widths of the bedding-parallel pore system.Conversely,the average pore lengths and widths are increased in the bedding-perpendicular direction.Besides,heating increases the cross-sectional area of pores in low-maturity oil shales,where this growth tendency fluctuates at<300℃ but becomes steady at>300℃.In addition,the orientation and shape of the newly-formed heating-induced pores follow the habit of the original pores and follow the initial probability distributions of pore orientation and shape.Herein,limited anisotropy is detected in pore direction and shape,indicating similar modes of evolution both bedding-parallel and bedding-normal.We propose a straightforward but robust model to describe evolution of pore system in low-maturity oil shales during heating.展开更多
The interface defects between the electron transport layer(ETL)and the perovskite layer,as well as the low ultraviolet(UV)light utilization rate of the perovskite absorption layer,pose significant challenges for the c...The interface defects between the electron transport layer(ETL)and the perovskite layer,as well as the low ultraviolet(UV)light utilization rate of the perovskite absorption layer,pose significant challenges for the commercialization of perovskite solar cells(PSCs).To address this issue,this paper proposes an innovative multifunctional interface modulation strategy by introducing aggregation-induced emission(AIE)molecule 5-[4-[1,2,2-tri[4-(3,5-dicarboxyphenyl)phenyl]ethylene]phenyl]benzene-1,3-dicarboxylic acid(H_(8)ETTB)at the SnO_(2)ETL/perovskite interface.Firstly,the interaction of H_(8)ETTB with the SnO_(2)surface,facilitated by its carboxyl groups,is effective in passivating surface defects caused by noncoord inated Sn and O vacancies.This interaction enhances the conductivity of the SnO_(2)film and adjusts energy levels,leading to enhanced charge carrier transport.Simultaneously,H_(8)ETTB can passivate noncoord inated Pb^(2+)ions at the perovskite interface,promoting perovskite crystallization and reducing the interface energy barrier,resulting in a perovskite film with low defects and high crystalline quality.More importantly,the H_(8)ETTB molecule,can convert UV light into light absorbable by the perovskite,thereby reducing damage caused by UV light and improving the device's utilization of UV.Consequently,the champion PSC based on SnO_(2)-H_(8)ETTB achieves an impressing efficiency of 23.32%and significantly improved photostability compared with the control device after continuous exposure to intense UV radiation.In addition,the Cs_(0.05)(FA_(0.95)MA_(0.05))_(0.95)Pb(I_(0.95)Br_(0.05))_(3)based device can achieve maximum efficiency of 24.01%,demonstrating the effectiveness and universality of this strategy.Overall,this innovative interface bridging strategy effectively tackles interface defects and low UV light utilization in PSCs,presenting a promising approach for achieving highly efficient and stable PSCs.展开更多
Quantitative relationship between nanosecond pulsed laser parameters and the characteristics of laser-generated ultrasonic waves in polycrystalline materials was evaluated.The high energy of the pulsed laser with a la...Quantitative relationship between nanosecond pulsed laser parameters and the characteristics of laser-generated ultrasonic waves in polycrystalline materials was evaluated.The high energy of the pulsed laser with a large irradiation spot simultaneously generated ultrasonic longitudinal and shear waves at the epicenter under the slight ablation regime.An optimized denoising technique based on wavelet thresholding and variational mode decomposition was applied to reduce noise in shear waves with a low signal-to-noise ratio.An approach for characterizing grain size was proposed using spectral central frequency ratio(SCFR)based on time-frequency analysis.The results demonstrate that the generation regime of ultrasonic waves is not solely determined by the laser power density;even at high power densities,a high energy with a large spot can generate an ultrasonic waveform dominated by the thermoelastic effect.This is ascribed to the intensification of the thermoelastic effect with the proportional increase in laser irradiation spot area for a given laser power density.Furthermore,both longitudinal and shear wave SCFRs are linearly related to grain size in polycrystalline materials;however,the shear wave SCFR is more sensitive to finer-grained materials.This study holds great significance for evaluating metal material properties using laser ultrasound.展开更多
Urine-derived stem cells(USCs)are derived from urine and harbor the potential of proliferation and multidirectional differentiation.Moreover,USCs could be reprogrammed into pluripotent stem cells[namely urine-derived ...Urine-derived stem cells(USCs)are derived from urine and harbor the potential of proliferation and multidirectional differentiation.Moreover,USCs could be reprogrammed into pluripotent stem cells[namely urine-derived induced pluripotent stem cells(UiPSCs)]through transcription factors,such as octamer binding transcription factor 4,sex determining region Y-box 2,kruppel-like factor 4,myelocytomatosis oncogene,and Nanog homeobox and protein lin-28,in which the first four are known as Yamanaka factors.Mounting evidence supports that USCs and UiPSCs possess high potential of neurogenic,myogenic,and osteogenic differentiation,indicating that they may play a crucial role in the treatment of neurological and musculoskeletal diseases.Therefore,we summarized the origin and physiological characteristics of USCs and UiPSCs and their therapeutic application in neurological and musculoskeletal disorders in this review,which not only contributes to deepen our understanding of hallmarks of USCs and UiPSCs but also provides the theoretical basis for the treatment of neurological and musculoskeletal disorders with USCs and UiPSCs.展开更多
[Objectives]This study was conducted to optimize the extraction process of total flavonoids from Penthorum chinense Pursh and compare their contents from different parts.[Methods]Single factor and orthogonal experimen...[Objectives]This study was conducted to optimize the extraction process of total flavonoids from Penthorum chinense Pursh and compare their contents from different parts.[Methods]Single factor and orthogonal experiments were designed to optimize the extraction process of total flavonoids from P.chinense Pursh with the volume fraction of ethanol,the ratio of material to liquid,heating reflux extraction time and extraction times as factors,and the content of total flavonoids as the index.A verification test was carried out.The optimized extraction process was adopted to compare the contents of total flavonoids from different parts of P.chinense Pursh.[Results]The best extraction process was extracting the powder of P.chinense Pursh for 2.0 h with 20 times of 55%ethanol by reflux twice.Under this condition,the contents of total flavonoids were 3.63%,8.90%,11.28%,and 4.36%from stems,leaves,flowers and whole grass of P.chinense Pursh,respectively.[Conclusions]The process is reasonable,feasible and stable,and can effectively extract total flavonoids from P.chinense Pursh.The contents of total flavonoids from different parts of P.chinense Pursh were quite different,and the value was higher in the leaves and flowers,so the proportions of leaves and flowers should be paid attention to in the industrial processing of P.chinense Pursh.展开更多
Major elements and carbon isotopes of dissolved inorganic carbon(DIC)have been measured in the waters of Changbaishan mountain,a volcanic area in northeastern China,between June and September 2016 to decipher the orig...Major elements and carbon isotopes of dissolved inorganic carbon(DIC)have been measured in the waters of Changbaishan mountain,a volcanic area in northeastern China,between June and September 2016 to decipher the origin of the CO_2 involved in chemical weathering reactions.Spatial variations of major elements ratios measured in water samples can be explained by a change of the chemical composition of the volcanic rocks between the volcanic cone(trachytes)and the basaltic shield as evidenced by the variations in the composition of these rocks.Hence,DIC results from the neutralization of CO_2 by silicate rocks.DIC concentrations vary from 0.3 to 2.5 mmol/L and carbon isotopic compositions of DIC measured in rivers vary from-14.2‰to 3.5‰.At a first order,the DIC transported by rivers is derived from the chemical weathering’s consumption of CO_2 with a magmatic origin,enriched in^(13)C(-5%)and biogenic soil CO_2 with lower isotopic compositions.The highest δ^(13)C values likely result from C isotopes fractionation during CO_2 degassing in rivers.A mass balance based on carbon isotopes suggest that the contribution of magmatic CO_2 varied from less than 20%to more than 70%.Uncertainties in this calculation associated with CO_2 degassing in rivers are difficult to quantify,and the consequence of CO_2 degassing would be an overestimation of the contribution of DIC derived from the neutralization of magmatic CO_2 by silicate rocks.展开更多
Background:Gongxuening capsule is a Chinese patent medicine for treating the dysfunctional uterine bleeding.We report a meta-analysis of the combination efficacy between conventional medicines with Chinese patent drug...Background:Gongxuening capsule is a Chinese patent medicine for treating the dysfunctional uterine bleeding.We report a meta-analysis of the combination efficacy between conventional medicines with Chinese patent drug Gongxuening in the treatment of dysfunctional uterine bleeding.Materials and methods:Seven databases were used to retrieve the relevant research of Chinese patent drug Gongxuening treatment for dysfunctional uterine bleeding from the databases establishment to 2020.The data were extracted independently by 2 researchers according to the described selection research.The meta-analysis was performed using RevMan5.3 software.Results:This study included 23 randomized trials,with a total of 1,836 dysfunctional uterine bleeding patients.Chinese patent drug Gongxuening could significantly improve the effective rate,and reduce endometrial thickness,control bleeding time,complete hemostasis time and sex hormone level,and no serious adverse events.Based on sensitivity analysis and subgroup analysis,the heterogeneity sources of endometrial thickness,complete hemostasis time and sex hormone level were found.In follicle stimulating hormone and luteinizing hormone,there was no significant difference between the two groups when Chinese patent drug Gongxuening combined with desogestrel ethinylestradiol.Conclusion:Conventional medicines combined with Chinese patent drug Gongxuening can make patients with dysfunctional uterine bleeding achieve better effective rate,and no serious adverse events occurred.展开更多
基金supported by the National Natural Science Foundation of China(31501977)the Sichuan Provincial Key R&D Project China(22ZDYF0194)the Double World-Class Project of Southwest Minzu University China(XM2023010)。
文摘This study aimed to investigate the dose-effect of iron on growth performance,antioxidant function.intestinal morphology,and mRNA expression of jejunal tight junction protein in 1-to21-d-old yellow-feathered broilers.A total of 7201-d-old yellow-feathered maleb roilers were allocated to 9 treatments with 8 replicate cages of 10 birds per cage.The dietary treatments were consisted of a basal diet(contained 79.6 mg Fe kg^(-1))supplemented with 0,20,40,60,80,160,320,640,and 1,280 mg Fe kg^(-1)in the form of FeSO_(4)·7H_(2)O.Compared with the birds in the control group,birds supplemented with 20mg Fe kg^(-1)had higher average daily gain(ADG)(P<0.0001).Adding 640 and 1,280 mg Fe kg^(-1)significantly decreased ADG(P<0.0001)and average daily feed intake(ADFI)(P<0.0001)compared with supplementation of 20mg Fe kg^(-1).Malondialdehyde(MDA)concentration in plasma and duodenum increased linearly(P<0.0001),but MDA concentration in liver and jejunum increased linearly(P<0.05)or quadratically(P<0.05)with increased dietary Fe concentration.The villus height(VH)in duodenum and jejunum,and the ratio of villus height to crypt depth(V/C)in duodenum decreased linearly(P?0.05)as dietary Feincreased.As dietary Fe increased,the jejunal relative mRNA abundance of claudin-1 decreased linearly(P=0.001),but the jejunal relative mRNA abundance of zona occludens-1(ZO-1)and occludin decreased linearly(P?0.05)or quadratically(P?0.05).Compared with the supplementation of 20 mg Fe kg^(-1),the supplementation of640 mg Fe kg^(-1)or higher increased(P?0.05)MDA concentrations in plasma,duodenum,and jejunum,decreased VH in the duodenum and jejunum,and the addition of 1,280 mg Fe kg^(-1)reduced(P?0.05)the jejunal tight junction protein(claudin-1,ZO-1,occludin)mRNA abundance.In summary,640 mg of supplemental Fe kg^(-1)or greater was associated with decreased growth performance,increased oxidative stress,disrupted intestinal morphology,and reduced mRNA expression of jejunal tight junction protein.
基金supported by the National Key Research and Development Program,China(Grant Nos.:2021YFC2101500 and 2021YFC2103900)the National Natural Science Foundation of China(Grant Nos.:22278335 and 21978236)the Natural Science Basic Research Program of Shaanxi,China(Grant No.:2023-JC-JQ-17).
文摘The gut microbiota plays a pivotal role in the immunomodulatory and protumorigenic microenvironment of colorectal cancer(CRC).However,the effect of ginsenoside Rk3(Rk3)on CRC and gut microbiota remains unclear.Therefore,the purpose of this study is to explore the potential effect of Rk3 on CRC from the perspective of gut microbiota and immune regulation.Our results reveal that treatment with Rk3 significantly suppresses the formation of colon tumors,repairs intestinal barrier damage,and regulates the gut microbiota imbalance caused by CRC,including enrichment of probiotics such as Akkermansia muciniphila and Barnesiella intestinihominis,and clearance of pathogenic Desulfovibrio.Subsequent metabolomics data demonstrate that Rk3 can modulate the metabolism of amino acids and bile acids,particularly by upregulating glutamine,which has the potential to regulate the immune response.Furthermore,we elucidate the regulatory effects of Rk3 on chemokines and inflammatory factors associated with group 3 innate lymphoid cells(ILC3s)and T helper 17(Th17)signaling pathways,which inhibits the hyperactivation of the Janus kinase-signal transducer and activator of transcription 3(JAK-STAT3)signaling pathway.These results indicate that Rk3 modulates gut microbiota,regulates ILC3s immune response,and inhibits the JAK-STAT3 signaling pathway to suppress the development of colon tumors.More importantly,the results of fecal microbiota transplantation suggest that the inhibitory effect of Rk3 on colon tumors and its regulation of ILC3 immune responses are mediated by the gut microbiota.In summary,these findings emphasize that Rk3 can be utilized as a regulator of the gut microbiota for the prevention and treatment of CRC.
基金financially supported by the National Key Research and Development Program of China(Grant No.2022YFE0129800)the National Natural Science Foundation of China(Grant No.42202204)。
文摘In-situ upgrading by heating is feasible for low-maturity shale oil,where the pore space dynamically evolves.We characterize this response for a heated substrate concurrently imaged by SEM.We systematically follow the evolution of pore quantity,size(length,width and cross-sectional area),orientation,shape(aspect ratio,roundness and solidity)and their anisotropy—interpreted by machine learning.Results indicate that heating generates new pores in both organic matter and inorganic minerals.However,the newly formed pores are smaller than the original pores and thus reduce average lengths and widths of the bedding-parallel pore system.Conversely,the average pore lengths and widths are increased in the bedding-perpendicular direction.Besides,heating increases the cross-sectional area of pores in low-maturity oil shales,where this growth tendency fluctuates at<300℃ but becomes steady at>300℃.In addition,the orientation and shape of the newly-formed heating-induced pores follow the habit of the original pores and follow the initial probability distributions of pore orientation and shape.Herein,limited anisotropy is detected in pore direction and shape,indicating similar modes of evolution both bedding-parallel and bedding-normal.We propose a straightforward but robust model to describe evolution of pore system in low-maturity oil shales during heating.
基金finically supported by the National Natural Science Foundation of China(62350054,12374379,12174152,12304462)the Foundation of National Key Laboratory(***202302011)。
文摘The interface defects between the electron transport layer(ETL)and the perovskite layer,as well as the low ultraviolet(UV)light utilization rate of the perovskite absorption layer,pose significant challenges for the commercialization of perovskite solar cells(PSCs).To address this issue,this paper proposes an innovative multifunctional interface modulation strategy by introducing aggregation-induced emission(AIE)molecule 5-[4-[1,2,2-tri[4-(3,5-dicarboxyphenyl)phenyl]ethylene]phenyl]benzene-1,3-dicarboxylic acid(H_(8)ETTB)at the SnO_(2)ETL/perovskite interface.Firstly,the interaction of H_(8)ETTB with the SnO_(2)surface,facilitated by its carboxyl groups,is effective in passivating surface defects caused by noncoord inated Sn and O vacancies.This interaction enhances the conductivity of the SnO_(2)film and adjusts energy levels,leading to enhanced charge carrier transport.Simultaneously,H_(8)ETTB can passivate noncoord inated Pb^(2+)ions at the perovskite interface,promoting perovskite crystallization and reducing the interface energy barrier,resulting in a perovskite film with low defects and high crystalline quality.More importantly,the H_(8)ETTB molecule,can convert UV light into light absorbable by the perovskite,thereby reducing damage caused by UV light and improving the device's utilization of UV.Consequently,the champion PSC based on SnO_(2)-H_(8)ETTB achieves an impressing efficiency of 23.32%and significantly improved photostability compared with the control device after continuous exposure to intense UV radiation.In addition,the Cs_(0.05)(FA_(0.95)MA_(0.05))_(0.95)Pb(I_(0.95)Br_(0.05))_(3)based device can achieve maximum efficiency of 24.01%,demonstrating the effectiveness and universality of this strategy.Overall,this innovative interface bridging strategy effectively tackles interface defects and low UV light utilization in PSCs,presenting a promising approach for achieving highly efficient and stable PSCs.
基金supported in part by the Natural Science Foundation of Shandong Province,China (Grant No.ZR2023ME073)the National Natural Science Foundation of China (Grant No.51805304)+1 种基金the Education Department of Shandong Province,China (Grant No.2022KJ130)Qilu University of Technology (Shandong Academy of Sciences),China (Grant Nos.2023PY009,2021JC02008 and 2022GH005)。
文摘Quantitative relationship between nanosecond pulsed laser parameters and the characteristics of laser-generated ultrasonic waves in polycrystalline materials was evaluated.The high energy of the pulsed laser with a large irradiation spot simultaneously generated ultrasonic longitudinal and shear waves at the epicenter under the slight ablation regime.An optimized denoising technique based on wavelet thresholding and variational mode decomposition was applied to reduce noise in shear waves with a low signal-to-noise ratio.An approach for characterizing grain size was proposed using spectral central frequency ratio(SCFR)based on time-frequency analysis.The results demonstrate that the generation regime of ultrasonic waves is not solely determined by the laser power density;even at high power densities,a high energy with a large spot can generate an ultrasonic waveform dominated by the thermoelastic effect.This is ascribed to the intensification of the thermoelastic effect with the proportional increase in laser irradiation spot area for a given laser power density.Furthermore,both longitudinal and shear wave SCFRs are linearly related to grain size in polycrystalline materials;however,the shear wave SCFR is more sensitive to finer-grained materials.This study holds great significance for evaluating metal material properties using laser ultrasound.
基金Supported by the Key Research and Development Program of Sichuan Science and Technology Agency,No.2020YFS0043Natural Science Foundation of Sichuan Province of China,No.2023NSFSC1567+1 种基金Sichuan University Innovation Research Project,No.2023SCUH0033Innovation Team at the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University,No.2022-CXTD-05.
文摘Urine-derived stem cells(USCs)are derived from urine and harbor the potential of proliferation and multidirectional differentiation.Moreover,USCs could be reprogrammed into pluripotent stem cells[namely urine-derived induced pluripotent stem cells(UiPSCs)]through transcription factors,such as octamer binding transcription factor 4,sex determining region Y-box 2,kruppel-like factor 4,myelocytomatosis oncogene,and Nanog homeobox and protein lin-28,in which the first four are known as Yamanaka factors.Mounting evidence supports that USCs and UiPSCs possess high potential of neurogenic,myogenic,and osteogenic differentiation,indicating that they may play a crucial role in the treatment of neurological and musculoskeletal diseases.Therefore,we summarized the origin and physiological characteristics of USCs and UiPSCs and their therapeutic application in neurological and musculoskeletal disorders in this review,which not only contributes to deepen our understanding of hallmarks of USCs and UiPSCs but also provides the theoretical basis for the treatment of neurological and musculoskeletal disorders with USCs and UiPSCs.
基金Supported by Key Research and Development Program of Sichuan Province(2022YFS0436)Natural Science Foundation of Sichuan Province(2022NSFSC1738)+4 种基金Science and Technology Planning Project of Luzhou City(2021-JYJ-109,2023SYF120)Special Project of Traditional Chinese Medicine Scientific Research of Sichuan Provincial Administration of Traditional Chinese Medicine(2020CP0029)Southwest Medical University-Luzhou Hospital of Traditional Chinese Medicine Base Project(2019-LH003)Open Subject of Luzhou Key Laboratory of Fine Chemical Application Technology(HYJY-2106-B)Southwest Medical University Undergraduate Student Innovation and Entrepreneurship Training Program(202310632074).
文摘[Objectives]This study was conducted to optimize the extraction process of total flavonoids from Penthorum chinense Pursh and compare their contents from different parts.[Methods]Single factor and orthogonal experiments were designed to optimize the extraction process of total flavonoids from P.chinense Pursh with the volume fraction of ethanol,the ratio of material to liquid,heating reflux extraction time and extraction times as factors,and the content of total flavonoids as the index.A verification test was carried out.The optimized extraction process was adopted to compare the contents of total flavonoids from different parts of P.chinense Pursh.[Results]The best extraction process was extracting the powder of P.chinense Pursh for 2.0 h with 20 times of 55%ethanol by reflux twice.Under this condition,the contents of total flavonoids were 3.63%,8.90%,11.28%,and 4.36%from stems,leaves,flowers and whole grass of P.chinense Pursh,respectively.[Conclusions]The process is reasonable,feasible and stable,and can effectively extract total flavonoids from P.chinense Pursh.The contents of total flavonoids from different parts of P.chinense Pursh were quite different,and the value was higher in the leaves and flowers,so the proportions of leaves and flowers should be paid attention to in the industrial processing of P.chinense Pursh.
基金supported by the National Natural Science Foundation of China through Grant No.41473023
文摘Major elements and carbon isotopes of dissolved inorganic carbon(DIC)have been measured in the waters of Changbaishan mountain,a volcanic area in northeastern China,between June and September 2016 to decipher the origin of the CO_2 involved in chemical weathering reactions.Spatial variations of major elements ratios measured in water samples can be explained by a change of the chemical composition of the volcanic rocks between the volcanic cone(trachytes)and the basaltic shield as evidenced by the variations in the composition of these rocks.Hence,DIC results from the neutralization of CO_2 by silicate rocks.DIC concentrations vary from 0.3 to 2.5 mmol/L and carbon isotopic compositions of DIC measured in rivers vary from-14.2‰to 3.5‰.At a first order,the DIC transported by rivers is derived from the chemical weathering’s consumption of CO_2 with a magmatic origin,enriched in^(13)C(-5%)and biogenic soil CO_2 with lower isotopic compositions.The highest δ^(13)C values likely result from C isotopes fractionation during CO_2 degassing in rivers.A mass balance based on carbon isotopes suggest that the contribution of magmatic CO_2 varied from less than 20%to more than 70%.Uncertainties in this calculation associated with CO_2 degassing in rivers are difficult to quantify,and the consequence of CO_2 degassing would be an overestimation of the contribution of DIC derived from the neutralization of magmatic CO_2 by silicate rocks.
基金This work was financially supported by the National Natural Science Foundation of China(no.81960696)Yunnan Science and Technology Department Project(no.2017FH001-097).
文摘Background:Gongxuening capsule is a Chinese patent medicine for treating the dysfunctional uterine bleeding.We report a meta-analysis of the combination efficacy between conventional medicines with Chinese patent drug Gongxuening in the treatment of dysfunctional uterine bleeding.Materials and methods:Seven databases were used to retrieve the relevant research of Chinese patent drug Gongxuening treatment for dysfunctional uterine bleeding from the databases establishment to 2020.The data were extracted independently by 2 researchers according to the described selection research.The meta-analysis was performed using RevMan5.3 software.Results:This study included 23 randomized trials,with a total of 1,836 dysfunctional uterine bleeding patients.Chinese patent drug Gongxuening could significantly improve the effective rate,and reduce endometrial thickness,control bleeding time,complete hemostasis time and sex hormone level,and no serious adverse events.Based on sensitivity analysis and subgroup analysis,the heterogeneity sources of endometrial thickness,complete hemostasis time and sex hormone level were found.In follicle stimulating hormone and luteinizing hormone,there was no significant difference between the two groups when Chinese patent drug Gongxuening combined with desogestrel ethinylestradiol.Conclusion:Conventional medicines combined with Chinese patent drug Gongxuening can make patients with dysfunctional uterine bleeding achieve better effective rate,and no serious adverse events occurred.
