Plant cell elongation depends on well-defined gene regulations,adequate nutrients,and timely cell wall modifications.Anther size is positively correlated with the number and viability of pollen grains,while little is ...Plant cell elongation depends on well-defined gene regulations,adequate nutrients,and timely cell wall modifications.Anther size is positively correlated with the number and viability of pollen grains,while little is known about molecular mechanisms underlying anther cell elongation.Here,we found that properly activated cell elongation regulators at transcriptional levels in loss-of-function ZmMs33 mutant(ms33-6038)anthers failed to promote maize anther elongation.ZmMs33 deficiency disrupted metabolic homeostasis mainly by inhibiting both photosynthesis in anther endothecium and lipid accumulation in anther tapetum.Importantly,ms33-6038 anthers displayed ectopic,premature and excessive secondary cell wall thickening in anther middle layer,which constrained cell elongation structurally and blocked nutrient flows across different anther wall layers.The metabolic disorder was only found in ms33-6038 mutant rather than several representative male-sterility lines at transcriptional and post-translational levels.Collectively,the disordered metabolisms and blocked nutrient flows defeated the activated cell elongation regulators,and finally inhibited anther elongation and growth with a unique‘‘idling effect”in ms33-6038 mutant.展开更多
Plant male reproduction is a fine-tuned developmental process that is susceptible to stressful environments and influences crop grain yields.Phytohormone signaling functions in control of plant normal growth and devel...Plant male reproduction is a fine-tuned developmental process that is susceptible to stressful environments and influences crop grain yields.Phytohormone signaling functions in control of plant normal growth and development as well as in response to external stresses,but the interaction or crosstalk among phytohormone signaling,stress response,and male reproduction in plants remains poorly understood.Cross-species comparison among 514 stress-response transcriptomic libraries revealed that ms33-6038,a genic male sterile mutant deficient in the Zm Ms33/Zm GPAT6 gene,displayed an excessive drought stress-like transcriptional reprogramming in anthers triggered mainly by disturbed jasmonic acid(JA)homeostasis.An increased level of JA appeared in Zm Ms33-deficient anthers at both meiotic and postmeiotic stages and activated genes involved in JA biosynthesis and signaling as well as genes functioning in JA-mediated drought response.Excessive accumulation of JA elevated expression level of a gene encoding a WRKY transcription factor that activated the Zm Ms33 promoter.These findings reveal a feedback loop of Zm Ms33-JA-WRKY-Zm Ms33 in controlling male sterility and JA-mediated stress response in maize,shedding light on the crosstalk of stress response and male sterility mediated by phytohormone homeostasis and signaling.展开更多
Mid-ocean ridge basalts(MORBs) are characterized by large variations in trace element compositions and isotopic ratios, which are difficult to be interpreted solely by using magmatic process such as partial melting of...Mid-ocean ridge basalts(MORBs) are characterized by large variations in trace element compositions and isotopic ratios, which are difficult to be interpreted solely by using magmatic process such as partial melting of a peridotitic mantle and subsequently fractional crystallization. Geochemical diversity of MORBs have been attributed to large-scale heterogeneity within the underlying mantle, and the heterogeneity might have been caused by addition of recycled crustal component, subcontinental lithosphere, metasomatized lithosphere and outer core contribution. In this study, we investigated the MORBs along the Mid-Atlantic Ridge(MAR) by estimating the temperature and pressure of partial melting, and comprehensively comparing trace element and isotope ratios. The data for MORBs from areas close to mantle plumes show large variations. Mantle plumes can affect mid-oceanic ridges 1 400 km away, but plume effects did not cover all of the ridge segments, and those segments without plume effects did not have any abnormalities in temperature, trace element or isotope ratios.We ascribed the above phenomena to result from the shapes of the plume flow, which we categorized as "pipelike channels" and "pancake-like channels". The "pancake-like channels" plumes affected the ambient mantle nondirectionally, but the range of the mantle affected by the "pipe-like channels" plumes were selective. Element ratios of MORBs reveal that the mantle source of the MORBs along the MAR is highly heterogeneous. We suggest that most of source heterogeneities of the MORBs may be due to the presence of subducted slab and delaminated lower crust in the source. In addition, the plume that carried materials from the core-mantle boundary may affect some of the segments.展开更多
Because of its significance for plant male fertility and,hence,direct impact on crop yield,pollen exine development has inspired decades of scientific inquiry.However,the molecularmechanismunderlying exine formation a...Because of its significance for plant male fertility and,hence,direct impact on crop yield,pollen exine development has inspired decades of scientific inquiry.However,the molecularmechanismunderlying exine formation and thickness remains elusive.In this study,we identified that a previously unrecognized repressor,ZmMS1/ZmLBD30,controls proper pollen exine development in maize.Using an ms1 mutant with aberrantly thickened exine,we cloned a male-sterility gene,ZmMs1,which encodes a tapetum-specific lateral organ boundary domain transcription factor,ZmLBD30.Weshowed thatZmMs1/ZmLBD30 is initially turned on by a transcriptional activation cascade of ZmbHLH51-ZmMYB84-ZmMS7,and then it serves as a repressor to shut down this cascade via feedback repression to ensure timely tapetal degeneration and proper level of exine.This activation-feedback repression loop regulating male fertility is conserved in maize and sorghum,and similar regulatory mechanism may also exist in other flowering plants such as rice and Arabidopsis.Collectively,these findings reveal a novel regulatory mechanism of pollen exine development by which a long-sought master repressor of upstream activators prevents excessive exine formation.展开更多
基金funded by the National Key Research and Development Program of China(2021YFF1000302)Fundamental Research Funds for the Central Universities of China(06500136)the Interdisciplinary Research Project for Young Teachers of USTB(Fundamental Research Funds for the Central Universities)(FRF-IDRY-20-038).
文摘Plant cell elongation depends on well-defined gene regulations,adequate nutrients,and timely cell wall modifications.Anther size is positively correlated with the number and viability of pollen grains,while little is known about molecular mechanisms underlying anther cell elongation.Here,we found that properly activated cell elongation regulators at transcriptional levels in loss-of-function ZmMs33 mutant(ms33-6038)anthers failed to promote maize anther elongation.ZmMs33 deficiency disrupted metabolic homeostasis mainly by inhibiting both photosynthesis in anther endothecium and lipid accumulation in anther tapetum.Importantly,ms33-6038 anthers displayed ectopic,premature and excessive secondary cell wall thickening in anther middle layer,which constrained cell elongation structurally and blocked nutrient flows across different anther wall layers.The metabolic disorder was only found in ms33-6038 mutant rather than several representative male-sterility lines at transcriptional and post-translational levels.Collectively,the disordered metabolisms and blocked nutrient flows defeated the activated cell elongation regulators,and finally inhibited anther elongation and growth with a unique‘‘idling effect”in ms33-6038 mutant.
基金funded by the National Key Research and Development Program of China (2021YFF1000302,2022YFF1003500, and 2022YFF1002400)the Fundamental Research Funds for the Central Universities of China (FRF-IDRY-20-038 and 06500136)the National Natural Science Foundation of China (31971958)。
文摘Plant male reproduction is a fine-tuned developmental process that is susceptible to stressful environments and influences crop grain yields.Phytohormone signaling functions in control of plant normal growth and development as well as in response to external stresses,but the interaction or crosstalk among phytohormone signaling,stress response,and male reproduction in plants remains poorly understood.Cross-species comparison among 514 stress-response transcriptomic libraries revealed that ms33-6038,a genic male sterile mutant deficient in the Zm Ms33/Zm GPAT6 gene,displayed an excessive drought stress-like transcriptional reprogramming in anthers triggered mainly by disturbed jasmonic acid(JA)homeostasis.An increased level of JA appeared in Zm Ms33-deficient anthers at both meiotic and postmeiotic stages and activated genes involved in JA biosynthesis and signaling as well as genes functioning in JA-mediated drought response.Excessive accumulation of JA elevated expression level of a gene encoding a WRKY transcription factor that activated the Zm Ms33 promoter.These findings reveal a feedback loop of Zm Ms33-JA-WRKY-Zm Ms33 in controlling male sterility and JA-mediated stress response in maize,shedding light on the crosstalk of stress response and male sterility mediated by phytohormone homeostasis and signaling.
基金The Basic Scientific Fund for National Public Research Institutes of China under contract No.2015G07the National Programme on Global Change and Air-Sea Interaction under contract Nos GASI-GEOGE-02+4 种基金the National Natural Science Foundation of China under contract Nos 41506079,41576052,41506068,41322036 and 41776070the AoShan Talents Program Supported by Qingdao National Laboratory for Marine Science and Technology under contract No.2015ASTP-ES16the Taishan Scholarship from Shandong Provincethe Research Grant of State Key Laboratory of Isotope Geochemistry,Guangzhou Institute of Geochemistry,Chinese Academy of Sciences under contract No.SKLIG-KF-14-03the China Postdoctoral Science Foundation under contract No.2016M592120
文摘Mid-ocean ridge basalts(MORBs) are characterized by large variations in trace element compositions and isotopic ratios, which are difficult to be interpreted solely by using magmatic process such as partial melting of a peridotitic mantle and subsequently fractional crystallization. Geochemical diversity of MORBs have been attributed to large-scale heterogeneity within the underlying mantle, and the heterogeneity might have been caused by addition of recycled crustal component, subcontinental lithosphere, metasomatized lithosphere and outer core contribution. In this study, we investigated the MORBs along the Mid-Atlantic Ridge(MAR) by estimating the temperature and pressure of partial melting, and comprehensively comparing trace element and isotope ratios. The data for MORBs from areas close to mantle plumes show large variations. Mantle plumes can affect mid-oceanic ridges 1 400 km away, but plume effects did not cover all of the ridge segments, and those segments without plume effects did not have any abnormalities in temperature, trace element or isotope ratios.We ascribed the above phenomena to result from the shapes of the plume flow, which we categorized as "pipelike channels" and "pancake-like channels". The "pancake-like channels" plumes affected the ambient mantle nondirectionally, but the range of the mantle affected by the "pipe-like channels" plumes were selective. Element ratios of MORBs reveal that the mantle source of the MORBs along the MAR is highly heterogeneous. We suggest that most of source heterogeneities of the MORBs may be due to the presence of subducted slab and delaminated lower crust in the source. In addition, the plume that carried materials from the core-mantle boundary may affect some of the segments.
基金supported by the National Key Research and Development Program of China(2022YFF1003500 to X.Wan,2022YFF1002400 and 2021YFF1000302 to S.W.,and 2022YFF1100501 to X.Wei)the National Natural Science Foundation of China(31771875 to X.Wan,31900610 to Q.H.31971958 to X.A.,and 31871702 to S.W.)+1 种基金the Fundamental Research Funds for the Central Universities of China(06500136 to X.Wan)the Beijing Nova Program(Z201100006820114 to Q.H.).
文摘Because of its significance for plant male fertility and,hence,direct impact on crop yield,pollen exine development has inspired decades of scientific inquiry.However,the molecularmechanismunderlying exine formation and thickness remains elusive.In this study,we identified that a previously unrecognized repressor,ZmMS1/ZmLBD30,controls proper pollen exine development in maize.Using an ms1 mutant with aberrantly thickened exine,we cloned a male-sterility gene,ZmMs1,which encodes a tapetum-specific lateral organ boundary domain transcription factor,ZmLBD30.Weshowed thatZmMs1/ZmLBD30 is initially turned on by a transcriptional activation cascade of ZmbHLH51-ZmMYB84-ZmMS7,and then it serves as a repressor to shut down this cascade via feedback repression to ensure timely tapetal degeneration and proper level of exine.This activation-feedback repression loop regulating male fertility is conserved in maize and sorghum,and similar regulatory mechanism may also exist in other flowering plants such as rice and Arabidopsis.Collectively,these findings reveal a novel regulatory mechanism of pollen exine development by which a long-sought master repressor of upstream activators prevents excessive exine formation.
