目的:基于HE染色的食管胃肿瘤切片图像构建细胞特征训练集,自动量化切片中的肿瘤浸润淋巴细胞(tumor-infiltrating lymphocytes,TILs),用以辅助预后研究。方法:纳入江苏泰兴214例食管胃交界处腺癌(adenocarcinoma of the esophagogastri...目的:基于HE染色的食管胃肿瘤切片图像构建细胞特征训练集,自动量化切片中的肿瘤浸润淋巴细胞(tumor-infiltrating lymphocytes,TILs),用以辅助预后研究。方法:纳入江苏泰兴214例食管胃交界处腺癌(adenocarcinoma of the esophagogastric junction,AEGJ)、256例胃腺癌(gastric adenocarcinoma,GAC)和752例食管鳞癌(esophageal squamous cell carcinoma,ESCC)病例,由病理医师建立包含肿瘤细胞、基质细胞及淋巴细胞特征的训练集,并使用二折验证,单细胞注释与自动识别结果之间的相关性检验,自动评估的TILs与人工评估的TILs比例的趋势性检验来评估细胞识别准确性。使用Kaplan-Meier法和Cox等比例风险模型探索预后应用价值。结果:该工具识别食管胃肿瘤TILs的准确性至少达到90%。分类器与病理医师对AEGJ、GAC和ESCC定量评估的总体相关系数分别为0.92、0.93和0.93,TILs的相关系数分别为0.94、0.95和0.95。三种肿瘤的TILs比例自动识别结果与人工分级一致。预后应用显示TILs比例为食管胃肿瘤的独立预后标志物。结论:基于食管胃癌细胞特征训练集的TILs浸润度评估工具可用于TILs比例的准确评估,具有良好的预后应用前景。展开更多
The evolution of microstructure during hot deformation is key to achieving good mechanical properties in aluminum alloys.We have developed a cellular automaton(CA) based model to simulate the microstructural evolution...The evolution of microstructure during hot deformation is key to achieving good mechanical properties in aluminum alloys.We have developed a cellular automaton(CA) based model to simulate the microstructural evolution in 7075 aluminum alloy during hot deformation.Isothermal compression tests were conducted to obtain material parameters for 7075 aluminum alloy,leading to the establishment of models for dislocation density,nucleation of recrystallized grains,and grain growth.Integrating these aspects with grain topological deformation,our CA model effectively predicts flow stress,dynamic recrystallization(DRX) volume fraction,and average grain size under diverse deformation conditions.A systematic comparison was made between electron back scattered diffraction(EBSD) maps and CA model simulated under different deformation temperatures(573 to 723 K),strain rates(0.001 to 1 s^(-1)),and strain amounts(30% to 70%).These analyses indicate that large strain,high temperature,and low strain rate facilitate dynamic recrystallization and grain refinement.The results from the CA model show good accuracy and predictive capability,with experimental error within 10%.展开更多
The effect of solution and aging treatments on microstructure and mechanical properties of warm-rolled 7075 alloy was investigated via optical microscope,electron backscattered diffraction,transmission electron micros...The effect of solution and aging treatments on microstructure and mechanical properties of warm-rolled 7075 alloy was investigated via optical microscope,electron backscattered diffraction,transmission electron microscopy and tensile tests.The 7075 alloy was subjected to solution treatments at 450℃for 1 h(ST1),490℃ for 1 h(ST2)and 1.5 h(ST3).Three aging routes were carried out on samples from ST2:one-step(A1),two-step(A2),and three-step aging(A3).The experimental results show mainly recrystallized equiaxed grains in ST1 and ST3 state but a combination of elongated and equiaxed grains in ST2 condition.Three aged alloys have similar microstructures of sample ST2 while the recrystallization frequency gets decreased after aging.The least recrystallization fraction occurs in A2 state.Three aged 7075 alloys all possess enhanced strength and plasticity.Precipitates characterization reveals the maximum strength is achieved in A2 sample as the matrix precipitates are composed mainly of smallηand manyη′phases.Aging route A2 appears preferable to other two aging conditions for attaining a pretty excellent combination of strength and plasticity.展开更多
文摘目的:基于HE染色的食管胃肿瘤切片图像构建细胞特征训练集,自动量化切片中的肿瘤浸润淋巴细胞(tumor-infiltrating lymphocytes,TILs),用以辅助预后研究。方法:纳入江苏泰兴214例食管胃交界处腺癌(adenocarcinoma of the esophagogastric junction,AEGJ)、256例胃腺癌(gastric adenocarcinoma,GAC)和752例食管鳞癌(esophageal squamous cell carcinoma,ESCC)病例,由病理医师建立包含肿瘤细胞、基质细胞及淋巴细胞特征的训练集,并使用二折验证,单细胞注释与自动识别结果之间的相关性检验,自动评估的TILs与人工评估的TILs比例的趋势性检验来评估细胞识别准确性。使用Kaplan-Meier法和Cox等比例风险模型探索预后应用价值。结果:该工具识别食管胃肿瘤TILs的准确性至少达到90%。分类器与病理医师对AEGJ、GAC和ESCC定量评估的总体相关系数分别为0.92、0.93和0.93,TILs的相关系数分别为0.94、0.95和0.95。三种肿瘤的TILs比例自动识别结果与人工分级一致。预后应用显示TILs比例为食管胃肿瘤的独立预后标志物。结论:基于食管胃癌细胞特征训练集的TILs浸润度评估工具可用于TILs比例的准确评估,具有良好的预后应用前景。
基金Funded by the Central Government Guides Local Funds for Science and Technology Development(No.YDZJSX20231A045)the Fundamental Research Program of Shanxi Province(Nos.202103021223288 and 202103021224282)。
文摘The evolution of microstructure during hot deformation is key to achieving good mechanical properties in aluminum alloys.We have developed a cellular automaton(CA) based model to simulate the microstructural evolution in 7075 aluminum alloy during hot deformation.Isothermal compression tests were conducted to obtain material parameters for 7075 aluminum alloy,leading to the establishment of models for dislocation density,nucleation of recrystallized grains,and grain growth.Integrating these aspects with grain topological deformation,our CA model effectively predicts flow stress,dynamic recrystallization(DRX) volume fraction,and average grain size under diverse deformation conditions.A systematic comparison was made between electron back scattered diffraction(EBSD) maps and CA model simulated under different deformation temperatures(573 to 723 K),strain rates(0.001 to 1 s^(-1)),and strain amounts(30% to 70%).These analyses indicate that large strain,high temperature,and low strain rate facilitate dynamic recrystallization and grain refinement.The results from the CA model show good accuracy and predictive capability,with experimental error within 10%.
基金Funded by the Key Research and Development Program of Shanxi Province (No.201903D121040)。
文摘The effect of solution and aging treatments on microstructure and mechanical properties of warm-rolled 7075 alloy was investigated via optical microscope,electron backscattered diffraction,transmission electron microscopy and tensile tests.The 7075 alloy was subjected to solution treatments at 450℃for 1 h(ST1),490℃ for 1 h(ST2)and 1.5 h(ST3).Three aging routes were carried out on samples from ST2:one-step(A1),two-step(A2),and three-step aging(A3).The experimental results show mainly recrystallized equiaxed grains in ST1 and ST3 state but a combination of elongated and equiaxed grains in ST2 condition.Three aged alloys have similar microstructures of sample ST2 while the recrystallization frequency gets decreased after aging.The least recrystallization fraction occurs in A2 state.Three aged 7075 alloys all possess enhanced strength and plasticity.Precipitates characterization reveals the maximum strength is achieved in A2 sample as the matrix precipitates are composed mainly of smallηand manyη′phases.Aging route A2 appears preferable to other two aging conditions for attaining a pretty excellent combination of strength and plasticity.
