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Krupple样因子6及诱导型一氧化氮合酶在铜绿假单胞菌上清液感染RAW264.7凋亡过程中的表达 被引量:4
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作者 刘梦茹 王雅琪 +3 位作者 张伟 姚富荣 苍林 柴文戍 《中国药理学通报》 CAS CSCD 北大核心 2019年第7期940-944,共5页
目的 检测Krupple样因子6(Krupple-like factor 6,KLF6)及诱导型一氧化氮合酶(inducible nitric oxide synthase, iNOS)在铜绿假单胞菌(pseudomonas aeruginosa,PA)上清液感染的小鼠单核巨噬细胞白血病细胞(RAW264.7)中的表达水平,探讨K... 目的 检测Krupple样因子6(Krupple-like factor 6,KLF6)及诱导型一氧化氮合酶(inducible nitric oxide synthase, iNOS)在铜绿假单胞菌(pseudomonas aeruginosa,PA)上清液感染的小鼠单核巨噬细胞白血病细胞(RAW264.7)中的表达水平,探讨KLF6及iNOS在PA上清液感染RAW264.7凋亡过程中的作用。方法 MTT法检测PA上清液对RAW264.7增殖的影响;流式细胞术检测凋亡率;Hoechst33342染色检测RAW264.7细胞核形态变化;Western blot检测PA上清液对KLF6、iNOS表达的影响。结果 PA上清液抑制RAW264.7的增殖,且其抑制作用呈浓度和时间依赖性,PA上清液能够明显增加RAW264.7的凋亡比例,促进KLF6、iNOS的表达。结论 PA能够抑制RAW264.7的增殖,其可能通过增加KLF6及iNOS的表达,从而诱导细胞凋亡。 展开更多
关键词 铜绿假单胞菌 RAW264.7 凋亡 KLF6 INOS 炎症
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Ultra-low temperature anodic bonding of silicon and glass based on nano-gap dielectric barrier discharge 被引量:1
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作者 yao fu-rong PAN Ming-qiang +2 位作者 ZHU Zong-jian LIU Ji-zhu WANG Yang-jun 《Journal of Central South University》 SCIE EI CAS CSCD 2021年第2期351-360,共10页
The article improves the process of dielectric barrier discharge(DBD)activated anode bonding.The treated surface was characterized by the hydrophilic surface test.The results showed that the hydrophilic angle was sign... The article improves the process of dielectric barrier discharge(DBD)activated anode bonding.The treated surface was characterized by the hydrophilic surface test.The results showed that the hydrophilic angle was significantly reduced under nano-gap conditions and the optimal discharge voltage was 2 kV.Then,the anodic bonding and dielectric barrier discharge activated bonding were performed in comparison experiments,and the bonding strength was characterized by tensile failure test.The results showed that the bonding strength was higher under the nano-gap dielectric barrier discharge.This process completed 110°C ultra-low temperature anodic bonding and the bonding strength reached 2 MPa.Finally,the mechanism of promoting bonding after activation is also discussed. 展开更多
关键词 dielectric barrier discharge anodic bonding ultra-low temperature
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